CN108745434A - A kind of liquid absorption device for preventing from carrying pollution between disk - Google Patents
A kind of liquid absorption device for preventing from carrying pollution between disk Download PDFInfo
- Publication number
- CN108745434A CN108745434A CN201810947353.0A CN201810947353A CN108745434A CN 108745434 A CN108745434 A CN 108745434A CN 201810947353 A CN201810947353 A CN 201810947353A CN 108745434 A CN108745434 A CN 108745434A
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- China
- Prior art keywords
- pipette tips
- headstock
- liquid absorption
- pollution
- liquid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Devices For Use In Laboratory Experiments (AREA)
Abstract
The present invention discloses a kind of liquid absorption device for preventing from carrying pollution between disk, including negative pressure suction device, the imbibition pipette tips device that is connect with negative pressure suction device, imbibition pipette tips device are mounted on the bottom of negative pressure suction device；Negative pressure suction device includes negative-pressure vacuum pump, vacuum suction system and pipe-line system；Vacuum suction system includes mandril, cover board, absorbs headstock, sealing clamp and mandril briquetting；The pipe-line system includes being mounted on the pipe joint absorbed on headstock, and pipe joint is connected with negative-pressure vacuum pump；Imbibition pipette tips device includes pipette tips suction nozzle, de- pipette tips plate, pipette tips positioning plate and several disposable pipette tips.The present invention can realize on the basis of liquid absorption device eliminate disk between carry the effect of pollution, reduce the loss of disposable pipette tips, shorten experimental period, ensure the accuracy of experimental result.Liquid absorption device of the present invention can need the liquid drawn as the corollary apparatus at liquid working station by the control at liquid working station, accuracy controlling.
Description
Technical field
The present invention relates to a kind of liquid absorption devices for preventing from carrying pollution between disk, belong to nucleic acid test liquid and draw field.
Background technology
The automation largely operated in Nucleic acid assays and cell experiment may be implemented in current liquid handling work station, because
This is widely used.Present global liquid working station, the mode of absorption liquid is only liquid injection pumping and takes, this
The volume aspirated of absorption mode is limited to the volume of liquid injection pump, has the following problems：(1) for the absorption of large volume waste liquid
It needs repeatedly to draw the effect that can be only achieved exhaustion waste liquid, increases experimental period, also increase the loss of disposable pipette tips, it is right
Experimental result has an impact；(2) reagent of more difficult absorption (such as ethyl alcohol can be attached on micropore wooden partition) needs are repeated as many times
The effect that draws or can be only achieved the step of adding quiescence in high temperature absorption, increases test period, and shadow is generated to experimental result
It rings；(3) liquid injection pump draws waste liquid in 96 orifice plates, is common waste-absorbing device, but is not suitable for batch experiment, less efficient,
And it will produce cross contamination.
The device that liquid assimilating effect in immunoassay field has been widely accepted is dishwashers, can greatly improve work
Make efficiency and work can property again, be one of immunoassay tool of standard.But it is only used for carrying out always immunologic
Test does not enter into the market of nucleic acid test, because its structure is easy to happen carrying pollution, causes detection of nucleic acids to occur wrong
Accidentally.Because nucleic acid test is extremely sensitive, it is far above immunoassay, any cross contamination (Crossover containimation) is all
Do not allow.Therefore, nucleic acid testing field lacks always a kind of efficient liquid assimilating tool.
Research before concentrates on cleaning dishwashers draw point, as a result fails to solve the problems, such as, even if because nucleic acid by
It diluting 1,000,000 times and still remains the risk for carrying pollution, cleaning is obviously unable to reach the effect thoroughly eliminated and carry pollution, and
Also custom coordinates disposable suction nozzle to carry out liquid operation to biotechnology worker with liquid-transfering gun, for conventional research, surveys
It is good enough for examination or application.But due to science and technology development, nucleic acid test and genomics increasingly show it is important
Effect, and it is many times required for the working method using high-throughput test or Large-scale Screening, thus various automations
Equipment start to occur and according to traditional " liquid-transfering gun-suction nozzle " pattern be unfolded, people is freed from complicated liquid feeding work
Out.
The prior art " liquid-transfering gun-suction nozzle " pattern can successfully solve to carry asking for pollution caused by common draw point imbibition
Topic；But either manual operations or automation equipment operation, cost are that the decline of imbibition efficiency and suction nozzle use number
The increase of amount is once required for the suction nozzle more renewed because often inhaling.If being changed without suction nozzle, though the effect of imbibition can be greatly improved
Rate, saves the consumption of suction nozzle and time, but must solve the problems, such as to carry pollution.
Invention content
In view of the above existing problems in the prior art, the present invention provides a kind of imbibition dresses for preventing from carrying pollution between disk
It sets, effectively prevent carrying pollution between disk, imbibition is efficient, disposably can largely draw waste liquid.
To achieve the goals above, a kind of liquid absorption device for preventing from carrying pollution between disk that the present invention uses, including it is negative
The imbibition pipette tips device pressed suction device, connect with negative pressure suction device, the imbibition pipette tips device are filled mounted on vacuum suction
The bottom set；
The negative pressure suction device includes negative-pressure vacuum pump, vacuum suction system and pipe-line system；
The vacuum suction system includes mandril, cover board, absorbs headstock, sealing clamp and mandril briquetting, and the cover board is logical
It crosses bolt and is mounted on the top for absorbing headstock, the sealing clamp is mounted on the lower section for absorbing headstock, the mandril by bolt
Mounted on absorbing on headstock, the mandril briquetting is movably arranged on the top of mandril；
The pipe-line system includes being mounted on the pipe joint absorbed on headstock, and the pipe joint pumps phase with negative-pressure vacuum
Even；
The imbibition pipette tips device includes pipette tips suction nozzle, de- pipette tips plate, pipette tips positioning plate, sealing rubber ring and several primary
Property pipette tips, the disposable pipette tips are mounted on the lower end of pipette tips suction nozzle, and the upper end of the pipette tips suction nozzle connect with de- pipette tips plate, institute
The top for stating de- pipette tips plate is equipped with pipette tips positioning plate, and pipette tips positioning plate is mounted on the bottom for absorbing headstock, and pipette tips positioning plate passes through
Sealing rubber ring is connect with sealing clamp.
As an improvement, the disposable pipette tips are removably mounted to the lower section of pipette tips suction nozzle.
As an improvement, the disposable pipette tips have 96, each disposable pipette tips are equidistantly regularly arranged respectively.
As an improvement, being equipped with sealing ring between the cover board and absorption headstock.
As an improvement, the de- pipette tips plate, the size of pipette tips positioning plate are identical, and de- pipette tips plate, pipette tips positioning plate difference
It is similar to the shape of ELISA Plate.
In addition, the application the present invention also provides a kind of liquid absorption device on nucleic acid and cell experiment.
Compared with prior art, the beneficial effects of the invention are as follows：
1) present invention can realize on the basis of liquid absorption device eliminate disk between carry the effect of pollution, reduce disposably
The loss of pipette tips, shortens experimental period, ensures the accuracy of experimental result.
2) liquid absorption device of the invention can pass through the control at liquid working station as the corollary apparatus at liquid working station
System, accuracy controlling need the liquid drawn.
3) liquid absorption device of the invention can accurately control the volume of draw solution, capture velocity.During the experiment can
The disposable liquid for siphoning away 96 orifice plates can improve conventional efficient and improve imbibition efficiency, shorten experimental period.
4) compared with traditional " one suction nozzle of liquid-transfering gun " system, the efficiency of imbibition greatly improved in the present invention, reduces imbibition
Time reduces the loss of suction nozzle, and realizes the elimination for carrying pollution.
Description of the drawings
Fig. 1 is the overall structure diagram of the present invention；
Fig. 2 is the partition structural schematic diagram of the present invention；
Fig. 3 is the exploded perspective view of the present invention.
In figure：1, disposable pipette tips, 2, pipette tips suction nozzle, 3, de- pipette tips plate, 4, pipe joint, 5, mandril, 6, pipette tips positioning
Plate, 7, cover board, 8, mandril briquetting, 9, absorb headstock, 10, sealing rubber ring, 11, sealing clamp, 12, sealing ring.
Specific implementation mode
In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention is carried out below further detailed
It describes in detail bright.However, it should be understood that the specific embodiments described herein are merely illustrative of the present invention, it is not limited to this hair
Bright range.
Unless otherwise defined, all technical terms and scientific terms used herein are led with the technology for belonging to the present invention
The normally understood meaning of technical staff in domain is identical, and used term is intended merely to retouch in the description of the invention herein
State the purpose of specific embodiment, it is not intended that in the limitation present invention.
As shown in Figure 1, Figure 2 and Figure 3, a kind of liquid absorption device for preventing from carrying pollution between disk, including negative pressure suction device,
The imbibition pipette tips device being connect with negative pressure suction device, the imbibition pipette tips device are mounted on the bottom of negative pressure suction device；
The negative pressure suction device includes negative-pressure vacuum pump, vacuum suction system and pipe-line system；
The vacuum suction system includes mandril 5, cover board 7, absorbs headstock 9, sealing clamp 11 and mandril briquetting 8, described
Cover board 7 is mounted on the top for absorbing headstock 9 by bolt, and the sealing clamp 11 is mounted on by bolt to be absorbed under headstock 9
Side, the mandril 5, which is mounted on, to be absorbed on headstock 9, and the mandril briquetting 8 is movably arranged on the top of mandril 5；
The pipe-line system includes being mounted on the pipe joint 4 absorbed on headstock 9, the pipe joint 4 and negative-pressure vacuum
Pump is connected；
If the imbibition pipette tips device include pipette tips suction nozzle 2, de- pipette tips plate 3, pipette tips positioning plate 6, sealing rubber ring 10 and
Dry disposable pipette tips 1, the disposable pipette tips 1 are mounted on the lower end of pipette tips suction nozzle 2, upper end and the de- rifle of the pipette tips suction nozzle 2
Head plate 3 connects, and the top of the de- pipette tips plate 3 is connect with pipette tips positioning plate 6, and pipette tips positioning plate 6 is mounted on the bottom for absorbing headstock 9
Portion, pipette tips positioning plate 6 are connect by sealing rubber ring 10 with sealing clamp 11.
The liquid absorption device of the present invention can pass through the control at liquid working station as the corollary apparatus at liquid working station
System, accuracy controlling need the liquid drawn.
As the improvement of embodiment, the disposable pipette tips 1 are removably mounted to the lower section of pipette tips suction nozzle 2, user
Just.
As the improvement of embodiment, the disposable pipette tips 1 have 96, and each disposable pipette tips 1 are equidistantly advised respectively
Then arrange.96 pipette tips can be dismantled simultaneously after drawing liquid, it is efficient, experimental period is shortened, in addition the specification of pipette tips suction nozzle 2
It is matched with various pipette tips specifications needed for Bioexperiment.
As the improvement of embodiment, the cover board 7 and absorb headstock 9 between be equipped with sealing ring 12, the service life of extension site,
Ensure the normal use of component.
As the improvement of embodiment, the de- pipette tips plate 3, the size of pipette tips positioning plate 6 are identical, and de- pipette tips plate 3, pipette tips
Positioning plate 6 is similar to the shape of ELISA Plate respectively.
Finally, liquid absorption device of the invention can be used in the automation mechanized operation of nucleic acid and the absorption liquid on cell experiment.
When imbibition, the pipette tips suction nozzle 2 in liquid absorption device is socketed multiple disposable pipette tips 1 through the invention, true by negative pressure
Sky pump connecting pipe connector 4 forms pressure difference, and liquid working stand control negative-pressure vacuum pumps to draw liquid.After drawing liquid, liquid
Body running station makes de- pipette tips plate 3 move down by controlling mandril briquetting 8, and control sheds disposable pipette tips 1.During drawing liquid, energy
The effective cross contamination for avoiding sample room.
The present invention can realize on the basis of liquid absorption device eliminate disk between carry the effect of pollution, reduce disposable rifle
The loss of head, shortens experimental period, ensures the accuracy of experimental result.The liquid absorption device of the present invention can be used as liquid working
The corollary apparatus stood can need the liquid drawn by the control at liquid working station, accuracy controlling.The liquid absorption device of the present invention
The volume of draw solution, capture velocity can be accurately controlled.The liquid that 96 orifice plates can disposably be siphoned away during the experiment, can carry
High conventional efficient improves imbibition efficiency, shortens experimental period.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modification, equivalent replacement or improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (6)
1. a kind of liquid absorption device for preventing from carrying pollution between disk, which is characterized in that including negative pressure suction device and vacuum suction
The imbibition pipette tips device of device connection, the imbibition pipette tips device are mounted on the bottom of negative pressure suction device；
The negative pressure suction device includes negative-pressure vacuum pump, vacuum suction system and pipe-line system；
The vacuum suction system includes mandril (5), cover board (7), absorbs headstock (9), sealing clamp (11) and mandril briquetting
(8), the cover board (7) is mounted on the top for absorbing headstock (9) by bolt, and the sealing clamp (11) is mounted on by bolt
The lower section of headstock (9) is absorbed, the mandril (5), which is mounted on, to be absorbed on headstock (9), and the mandril briquetting (8) is movably arranged on top
The top of bar (5)；
The pipe-line system includes being mounted on the pipe joint (4) absorbed on headstock (9), and the pipe joint (4) and negative pressure are true
Sky pump is connected；
The imbibition pipette tips device includes pipette tips suction nozzle (2), de- pipette tips plate (3), pipette tips positioning plate (6), sealing rubber ring (10)
With several disposable pipette tips (1), the disposable pipette tips (1) are mounted on the lower end of pipette tips suction nozzle (2), the pipette tips suction nozzle (2)
Upper end connect with de- pipette tips plate (3), the top of the de- pipette tips plate (3) is equipped with pipette tips positioning plate (6), pipette tips positioning plate (6)
Mounted on the bottom for absorbing headstock (9), pipette tips positioning plate (6) is connect by sealing rubber ring (10) with sealing clamp (11).
2. a kind of liquid absorption device for preventing from carrying pollution between disk according to claim 1, which is characterized in that described primary
Property pipette tips (1) are removably mounted to the lower section of pipette tips suction nozzle (2).
3. a kind of liquid absorption device for preventing from carrying pollution between disk according to claim 2, which is characterized in that described one
Secondary property pipette tips (1) have 96, and each disposable pipette tips (1) are equidistantly regularly arranged respectively.
4. a kind of liquid absorption device for preventing from carrying pollution between disk according to claim 1, which is characterized in that the cover board
(7) sealing ring (12) is equipped between absorption headstock (9).
5. a kind of liquid absorption device for preventing from carrying pollution between disk according to claim 1, which is characterized in that the de- rifle
Head plate (3), the size of pipette tips positioning plate (6) are identical, and de- pipette tips plate (3), pipette tips positioning plate (6) respectively with the shape of ELISA Plate
It is similar.
6. a kind of according to the liquid absorption device that can prevent from carrying between disk pollution described in claim any one of 1-5, it is characterised in that：Institute
State application of the liquid absorption device that pollution is carried between can preventing disk on nucleic acid and cell experiment.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2018107557230 | 2018-07-11 | ||
| CN201810755723 | 2018-07-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108745434A true CN108745434A (en) | 2018-11-06 |
Family
ID=63967183
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810947353.0A Withdrawn CN108745434A (en) | 2018-07-11 | 2018-08-17 | A kind of liquid absorption device for preventing from carrying pollution between disk |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108745434A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112170373A (en) * | 2020-10-20 | 2021-01-05 | 西安交通大学医学院第一附属医院 | Full-automatic liquid-transfering suction head washs integrative device of dress box of drying |
-
2018
- 2018-08-17 CN CN201810947353.0A patent/CN108745434A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112170373A (en) * | 2020-10-20 | 2021-01-05 | 西安交通大学医学院第一附属医院 | Full-automatic liquid-transfering suction head washs integrative device of dress box of drying |
| CN112170373B (en) * | 2020-10-20 | 2024-04-19 | 西安交通大学医学院第一附属医院 | Full-automatic pipetting head washs stoving dress box integrative device |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181106 |