CN108743736A - Rhizoma valerianae latifoliae extract is preparing the application in restoring to be damaged intestinal villi or alleviating gut muco-membranous barrier damage medicine - Google Patents
Rhizoma valerianae latifoliae extract is preparing the application in restoring to be damaged intestinal villi or alleviating gut muco-membranous barrier damage medicine Download PDFInfo
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- CN108743736A CN108743736A CN201810241479.6A CN201810241479A CN108743736A CN 108743736 A CN108743736 A CN 108743736A CN 201810241479 A CN201810241479 A CN 201810241479A CN 108743736 A CN108743736 A CN 108743736A
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- extract
- rhizoma valerianae
- valerianae latifoliae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/84—Valerianaceae (Valerian family), e.g. valerian
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The invention discloses a kind of Rhizoma valerianae latifoliae extracts to prepare the application in restoring to be damaged intestinal villi or alleviating gut muco-membranous barrier damage medicine；The preparation method of the Rhizoma valerianae latifoliae extract includes the following steps：Jatamans valeriana rhizome is taken, the alcohol at normal temperature for being 95% with concentration of volume percent extracts, and concentrates leaching liquor, obtains medicinal extract；The medicinal extract is dissolved with ethyl acetate, filter, by gained filtrate dry to get.It is an advantage of the invention that：Obtained Rhizoma valerianae latifoliae extract can restore impaired intestinal villi or alleviate gut muco-membranous barrier damage.
Description
Technical field
The invention belongs to field of pharmaceutical biology, and in particular to a kind of Rhizoma valerianae latifoliae extract restores to be damaged intestinal villi preparing
Or the application in alleviation gut muco-membranous barrier damage medicine.
Background technology
Chinese medicine jatamans valeriana rhizome is the dry rhizome of Valerianaceae valerian jatamans valeriana rhizome Valeriana jatamansi Jones
And root, there is abundant medical value.First recorded in《Compendium of Materia Medica》：" warding off pestilence, middle evil ".《Yunnan Chinese herbal medicine》It records：It " controls and disappears
Change bad, infantile cough, infantile malnutrition due to digestive disturbances or intestinalparasites, influenza, malaria ", it is one of common drug in Yunnan Province.It is jatamans valeriana rhizome pharmacological property slight bitter, pungent, warm
With the effect of Chinese Traditional Medicine is thought with regulating qi-flowing for relieving pain, and help digestion antidiarrheal, dispelling wind and eliminating dampness, tranquillizing the mind by relieving convulsion.It is mainly used for treating
Abdominal distention, accumulation of food in the stomach and intes tine due to indigestion, diarrhea dysentery, arthralgia pain due to rheumatism, soreness and weakness of waist and knees and insomnia etc..In subsidiary formula, jatamans valeriana rhizome can be used for controlling
Having a heatstroke gas pain is treated, traumatic injury, activating blood circulation, muscle and bone ache, consumptive disease, which is hindered, coughs, and walks table cold dispelling and cold air；Abdominal pain is rushed down further for vomitting,
Peratodynia, rheumatic numbness and cures cold etc. and all to have longer usage history.The exploitation of jatamans valeriana rhizome medical value is more next in recent years
It is more taken seriously, numerous at present clinical research and clinical application example show to rush down clever conjunction with the autumn made of jatamans valeriana rhizome simple
Agent has significant curative effect for treating baby diarrhea.
Currently, more in plantation etc. research by raw material of jatamans valeriana rhizome, but there is not yet jatamans valeriana rhizome active principle for extensive
It is damaged intestinal villi again or alleviates the research report of gut muco-membranous barrier damage.
Invention content
The object of the present invention is to provide a kind of Rhizoma valerianae latifoliae extracts to restore to be damaged intestinal villi or alleviate enteron aisle to stick in preparation
Application in envelope barrier damage medicine.
For achieving the above object, the technical solution adopted in the present invention is：
A kind of Rhizoma valerianae latifoliae extract is in preparing and restoring to be damaged intestinal villi or alleviate gut muco-membranous barrier damage medicine
Using；The preparation method of the Rhizoma valerianae latifoliae extract includes the following steps：Jatamans valeriana rhizome is taken, is 95% with concentration of volume percent
Alcohol at normal temperature extracts, and concentrates leaching liquor, obtains medicinal extract；The medicinal extract is dissolved with ethyl acetate, filters, gained filtrate is dried,
To obtain the final product.
The present invention also provides a kind of Rhizoma valerianae latifoliae extracts to prepare the impaired intestinal villi of recovery or alleviate intestinal mucosa screen
Hinder the application in damage medicine；The preparation method of the Rhizoma valerianae latifoliae extract includes the following steps：Jatamans valeriana rhizome is taken, volume basis is used
The alcohol at normal temperature that specific concentration is 95% extracts, and concentrates leaching liquor, obtains medicinal extract；The medicinal extract is dissolved with ethyl acetate, is filtered, it will
Gained filtrate dry, be added petroleum ether dissolution, by petroleum ether dissolution liquid dry to get.
The present invention also provides a kind of Rhizoma valerianae latifoliae extracts to prepare the impaired intestinal villi of recovery or alleviate intestinal mucosa screen
Hinder the application in damage medicine；The preparation method of the Rhizoma valerianae latifoliae extract includes the following steps：Jatamans valeriana rhizome is taken, volume basis is used
The alcohol at normal temperature that specific concentration is 95% extracts, and concentrates leaching liquor, obtains medicinal extract；The medicinal extract is dissolved with ethyl acetate, is filtered, it will
Gained filtrate dry, be added petroleum ether dissolution, then by insoluble in petroleum ether it is partially dried to get.
Preferably, in the above-mentioned several method for preparing Rhizoma valerianae latifoliae extract, the number of the extraction is 1-5 times, every time
The time of extraction is 0.5-5 hours；The number that the ethyl acetate dissolving is added is 1-5 times；The petroleum ether dissolution is added
1-5 times of number.
The present invention has following technical advantage：
Jatamans valeriana rhizome is extracted with 95% alcohol at normal temperature, then is extracted with ethyl acetate, obtained Rhizoma valerianae latifoliae extract energy
Restore to be damaged intestinal villi or alleviates gut muco-membranous barrier damage.
Description of the drawings
Fig. 1 is the HE coloration results of Control group sample effects；
Fig. 2 is the HE coloration results of Model group sample effects；
Fig. 3 is the HE coloration results of positive drug smecta group sample effect；
Fig. 4 is the HE coloration results of VJ01 low dose group sample effects；
Fig. 5 is the HE coloration results of VJ01 middle dose group sample effects；
Fig. 6 is the HE coloration results of VJ01 high dose group sample effects；
Fig. 7 is the HE coloration results of VJ02 low dose group sample effects；
Fig. 8 is the HE coloration results of VJ02 middle dose group sample effects；
Fig. 9 is the HE coloration results of VJ02 high dose group sample effects；
Figure 10 is the HE coloration results of VJ03 low dose group sample effects；
Figure 11 is the HE coloration results of VJ03 middle dose group sample effects；
Figure 12 is the HE coloration results of VJ03 high dose group sample effects；
Figure 13 is the Occludin protein groups results of Control group sample effects；
Figure 14 is the Occludin protein groups results of Model group sample effects；
Figure 15 is the Occludin protein groups results of positive drug smecta group sample effect；
Figure 16 is the Occludin protein groups results of VJ01 low dose group sample effects；
Figure 17 is the Occludin protein groups results of VJ01 middle dose group sample effects；
Figure 18 is the Occludin protein groups results of VJ01 high dose group sample effects；
Figure 19 is the Occludin protein groups results of VJ02 low dose group sample effects；
Figure 20 is the Occludin protein groups results of VJ02 middle dose group sample effects；
Figure 21 is the Occludin protein groups results of VJ02 high dose group sample effects；
Figure 22 is the Occludin protein groups results of VJ03 low dose group sample effects；
Figure 23 is the Occludin protein groups results of VJ03 middle dose group sample effects；
Figure 24 is the Occludin protein groups results of VJ03 high dose group sample effects.
Specific implementation mode
The specific implementation mode of the present invention is described further below.It should be noted that for these implementations
The explanation of mode is used to help understand the present invention, but does not constitute limitation of the invention.In addition, invention described below
Involved technical characteristic can be combined with each other as long as they do not conflict with each other in each embodiment.
Embodiment 1
A kind of preparation method of Rhizoma valerianae latifoliae extract, includes the following steps：
Jatamans valeriana rhizome is taken, the alcohol at normal temperature for being 95% with concentration of volume percent extracts, and concentrates leaching liquor, obtains medicinal extract；By institute
It states medicinal extract to be dissolved with ethyl acetate, filter, gained filtrate is dried to get (VJ03).
Embodiment 2
A kind of preparation method of Rhizoma valerianae latifoliae extract, includes the following steps：
Jatamans valeriana rhizome is taken, the alcohol at normal temperature for being 95% with concentration of volume percent extracts, and concentrates leaching liquor, obtains medicinal extract；By institute
It states medicinal extract to be dissolved with ethyl acetate, filters, gained filtrate is dried, petroleum ether dissolution is added, petroleum ether dissolution liquid is dried, i.e.,
It obtains (VJ01).
Embodiment 3
A kind of preparation method of Rhizoma valerianae latifoliae extract, includes the following steps：
Jatamans valeriana rhizome is taken, the alcohol at normal temperature for being 95% with concentration of volume percent extracts, and concentrates leaching liquor, obtains medicinal extract；By institute
It states medicinal extract to be dissolved with ethyl acetate, filters, gained filtrate is dried, petroleum ether dissolution is added, it then will be insoluble in petroleum ether
It is partially dried to get (VJ02).
For the technique effect of the verification present invention, spy makees following tests：
By embodiment 1-3 obtained component VJ03, VJ01 and VJ02, mouse test is carried out respectively.
It takes mouse to be randomly divided into 12 groups, is marked with picric acid.Overnight fasting, free water.Gastric infusion.Normal group
Normal saline 0.3ml is given with model group, positive drug group is given dioctahedral smectite 3.6g/kg, VJ01 and VJ03 administration group and set
Low concentration group (1.75g/kg), middle concentration group (3.5g/kg), high concentration group (7g/kg) are set, low concentration group is arranged in VJ02 administration groups
(0.875g/kg), middle concentration group (1.75g/kg), high concentration group (3.5g/kg).Positive drug dioctahedral smectite, VJ01 be middle high group low,
The low middle height of VJ02 organizes middle high group administered volume (ml)=0.012* weight (g) low with VJ03, after 30min is administered, except normal control
Group is outer, and the equal gavage of each group gives castor oil 0.2ml/ only, then grid bottom single cage is used to raise, and observes 6h.Then animal is put to death, Gu
Due to autopsy table, abdominal cavity is opened, dissection takes out small intestine, enteral is washed down away from clip ileum about 5cm, physiological saline at ileocecus 5cm
It is tolerant, two parts are cut into, a part is put into EP pipes, is placed in -80 DEG C of refrigerators and preserves, and a part is put into paraformaldehyde fixed, use
In detection.
Interpretation of result：
As shown in figs. 1-12, Control groups mouse proximal segment Morphological Indices of Duodenum Villus is regular, a small amount of interstitial, suede in villus axis
Hair simple columnar epithelium marshalling, minute quantity acidophile degeneration, between epithelium or top of villi occasionally has lymphocytic infiltration.
The apparent oedema of the most intestinal villis of Model groups, the fusion of part villus；The apparent oedema of villus axis interstitial, hyperemia exudation, great Liang Lin
Bar cellular infiltration；Boundary is unclear between columnar epithelial cell, there is more acidophilia lesion and lymphocytic infiltration, part villus
Epithelium necrosis, falls off.Positive drug smecta group villus oedema improves, and inflammatory cell infiltration phenomenon is still more apparent, visible in enteric cavity
The chorionic villi to fall off.VJ01, VJ02, VJ03 group, intestinal villi damaged structure are obviously restored, and part top of villi is still visible
Columnar cell detaches with interstitial, or even missing, a small amount of capillary injection, lymphocytic infiltration.High dose and middle dose group are big
Rarely seen pole exudation and lymphocytic infiltration, columnar epithelial cell arrangement are more neat in the intestinal villi of part.
As shown in figures 13-24, Occludin protein expressions are in immunohistochemistry picture in enteron aisle villus epithelial cells slurry
Brown color, gray value is higher to illustrate that expression quantity is more.It is shown by figure, each group has expression, but it is variant to express positive degree.
Villus periphery brown color is deeper in Control groups, and Model groups are shallower.Positive drug smecta group protein expression increases, VJ01,
Occludin protein expressions in VJ02, VJ03 group have increase, description of test, these extractions of jatamans valeriana rhizome compared to Model groups
Object can partly alleviate the damage of gut muco-membranous barrier.
Embodiments of the present invention are explained in detail above, but the present invention is not limited to described embodiments.It is right
For those skilled in the art, in the case where not departing from the principle of the invention and spirit, these embodiments are carried out more
Kind change, modification, replacement and modification, still fall in protection scope of the present invention.
Claims (3)
1. a kind of Rhizoma valerianae latifoliae extract answering in preparing the impaired intestinal villi of recovery or alleviating gut muco-membranous barrier damage medicine
With；The preparation method of the Rhizoma valerianae latifoliae extract includes the following steps：Jatamans valeriana rhizome is taken, the second for being 95% with concentration of volume percent
Alcohol room temperature extracts, and concentrates leaching liquor, obtains medicinal extract；The medicinal extract is dissolved with ethyl acetate, filters, gained filtrate is dried, i.e.,
?.
2. a kind of Rhizoma valerianae latifoliae extract answering in preparing the impaired intestinal villi of recovery or alleviating gut muco-membranous barrier damage medicine
With；The preparation method of the Rhizoma valerianae latifoliae extract includes the following steps：Jatamans valeriana rhizome is taken, the second for being 95% with concentration of volume percent
Alcohol room temperature extracts, and concentrates leaching liquor, obtains medicinal extract；The medicinal extract is dissolved with ethyl acetate, filters, gained filtrate is dried, is added
Enter petroleum ether dissolution, by petroleum ether dissolution liquid dry to get.
3. a kind of Rhizoma valerianae latifoliae extract answering in preparing the impaired intestinal villi of recovery or alleviating gut muco-membranous barrier damage medicine
With；The preparation method of the Rhizoma valerianae latifoliae extract includes the following steps：Jatamans valeriana rhizome is taken, the second for being 95% with concentration of volume percent
Alcohol room temperature extracts, and concentrates leaching liquor, obtains medicinal extract；The medicinal extract is dissolved with ethyl acetate, filters, gained filtrate is dried, is added
Enter petroleum ether dissolution, then by insoluble in petroleum ether it is partially dried to get.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810241479.6A CN108743736A (en) | 2018-03-22 | 2018-03-22 | Rhizoma valerianae latifoliae extract is preparing the application in restoring to be damaged intestinal villi or alleviating gut muco-membranous barrier damage medicine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810241479.6A CN108743736A (en) | 2018-03-22 | 2018-03-22 | Rhizoma valerianae latifoliae extract is preparing the application in restoring to be damaged intestinal villi or alleviating gut muco-membranous barrier damage medicine |
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| CN108743736A true CN108743736A (en) | 2018-11-06 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1396166A (en) * | 2001-07-12 | 2003-02-12 | 中国人民解放军第二军医大学 | Process for preparing general valerianin |
| CN102133368A (en) * | 2011-03-22 | 2011-07-27 | 唐秋海 | Quality detection method for intestines and stomach easing capsule |
| CN103599144A (en) * | 2013-10-22 | 2014-02-26 | 浙江中医药大学 | Preparation method for effective part of valerianajatamansi epoxy iridoid ester |
| CN106008427A (en) * | 2016-07-01 | 2016-10-12 | 郑飞珍 | Novel sesquiterpene lactones compound, and preparation method and medical application thereof |
-
2018
- 2018-03-22 CN CN201810241479.6A patent/CN108743736A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1396166A (en) * | 2001-07-12 | 2003-02-12 | 中国人民解放军第二军医大学 | Process for preparing general valerianin |
| CN102133368A (en) * | 2011-03-22 | 2011-07-27 | 唐秋海 | Quality detection method for intestines and stomach easing capsule |
| CN103599144A (en) * | 2013-10-22 | 2014-02-26 | 浙江中医药大学 | Preparation method for effective part of valerianajatamansi epoxy iridoid ester |
| CN106008427A (en) * | 2016-07-01 | 2016-10-12 | 郑飞珍 | Novel sesquiterpene lactones compound, and preparation method and medical application thereof |
Non-Patent Citations (3)
| Title |
|---|
| MILE778: "《https://www.doc88.com/p-2876155795356.html?s=rel&id=3》", 23 June 2013, 道客巴巴 * |
| 赵春杰: "《中药学专业知识（二）》", 30 April 2012, 人民军医出版社 * |
| 邱峰: "《天然药物化学》", 30 September 2011, 上海科学技术出版社 * |
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Application publication date: 20181106 |