CN108743629A - The preparation method of lignans constituents in a kind of lepidogrammitis drymoglossoides Ching - Google Patents
The preparation method of lignans constituents in a kind of lepidogrammitis drymoglossoides Ching Download PDFInfo
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- CN108743629A CN108743629A CN201810821726.XA CN201810821726A CN108743629A CN 108743629 A CN108743629 A CN 108743629A CN 201810821726 A CN201810821726 A CN 201810821726A CN 108743629 A CN108743629 A CN 108743629A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/11—Pteridophyta or Filicophyta (ferns)
- A61K36/12—Filicopsida or Pteridopsida
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- A—HUMAN NECESSITIES
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a kind of preparation methods of lignans constituents in lepidogrammitis drymoglossoides Ching, and steps are as follows：By lepidogrammitis drymoglossoides Ching pulverizing medicinal materials, 40 mesh sieve is crossed, dry 23-25h, carries out ultrasonic extraction at 55-65 DEG C after being 65-75% alcohol solution dippings 47-49h with volume fraction, extracts 2-4 times, each 40-50min, and filtering merges extracting solution, is concentrated under reduced pressure to give medicinal extract；, through large pore resin absorption column, water, ethanol gradient elution will be used successively, collect 70% alcohol elution, then detach through silica gel column chromatography, use volume ratio 90 successively after the appropriate water dissolution of medicinal extract:1,75:1,45:1,26:1,14:1,5:1 chloroform-methanol elution, collects 14:1 elution fraction, concentrates to obtain the final product.Method provided by the invention can quickly obtain lignans constituents in lepidogrammitis drymoglossoides Ching, which has antibacterial, antiviral, antioxidant activity, can be used for drug preparation.
Description
Technical field
The present invention relates to a kind of preparation methods of lignans constituents in lepidogrammitis drymoglossoides Ching.
Background technology
Lepidogrammitis drymoglossoides Ching [Lepidogrammitis drymoglossoides (Baker) Ching, Polypodiaceae]
For the herb of fern Polypodiaceae dominoes Cyclosorus plant, root-like stock it is elongated it is horizontal walk, be drilled shape brown lanceolar scale with teeth.Spore
Telium is round, respectively in a row along master pulse both sides, between master pulse and limb.Ye Yuansheng, at a distance of 1.5-5 centimetres, two types；No
Leaf oblong is educated to oval, and long 1-2 centimetres or slightly long, round end or blunt circle head, base portion wedge shape, there are few handle, full edges；Fertile frond ligule
Or lanceolar, 3-6 centimetres long, wide not as good as 1 centimetre, base portion pinch, there are few handle or tool short handles, sometimes with sterile frond similar shape, meat
Matter, do after keratin, above it is smooth, below dredge by scale.
Be distributed widely in the provinces such as China Yangtze river basin and Guizhou, Guangdong, Guangxi, include flavones, alkaloid, sterol,
Amino acid and sugared isoreactivity ingredient.With all herbal medicine, there is the effect of clearing heat and detoxicating, dampness removing dispersing blood stasis, be used for sore-throat, lung heat cough
Blood, rheumatic arthritis, lymphnoditis, cholecystitis, urolithiasis, traumatic injury, serious case of furuncle carbuncle swells.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of lignans constituents in lepidogrammitis drymoglossoides Ching.
The present invention is realized by following technical solution：
The preparation method of lignans constituents, includes the following steps in a kind of lepidogrammitis drymoglossoides Ching：By lepidogrammitis drymoglossoides Ching pulverizing medicinal materials, 40 mesh are crossed
Sieve, dry 23-25h, ultrasonic extraction is carried out after being 65-75% alcohol solution dippings 47-49h with volume fraction at 55-65 DEG C,
Extraction 2-4 times, each 40-50min, filtering merge extracting solution, are concentrated under reduced pressure to give medicinal extract；After the appropriate water dissolution of medicinal extract
Through large pore resin absorption column, successively with water, 20%, 40%, 60%, 70%, 80%, 95% ethanol gradient elution, collects 70% ethyl alcohol and wash
De- position, then detached through silica gel column chromatography, volume ratio 90 is used successively:1,75:1,45:1,26:1,14:1,5:1 chloroform-methanol
Elution collects 14:1 elution fraction, concentrates to obtain the final product.
Preferably, it in the preparation method, is dried for 24 hours at 60 DEG C.
Preferably, it is 70% alcohol solution dipping 48h with volume fraction in the preparation method.
Preferably, it in the preparation method, extracts 3 times.
Preferably, in the preparation method, 45min is extracted every time.
Preferably, in the preparation method, 1kg lepidogrammitis drymoglossoides Chings are extracted using 11-13L solvents.
Preferably, in the preparation method, by medicinal extract first to use cotton filtering to be inhaled again with macropore after appropriate water dissolution
Attached resin column chromatography separation.
Preferably, in the preparation method, the 6-10mL water dissolutions of 1g medicinal extract.
Preferably, in the preparation method, the large pore resin absorption column is D101 types.
The technology of the present invention effect：
Method provided by the invention can quickly obtain lignans constituents in lepidogrammitis drymoglossoides Ching, which has antibacterial, antiviral, anti-
Oxidation activity can be used for drug preparation.
Specific implementation mode
The essentiality content of the present invention is specifically introduced with reference to embodiment.
Embodiment 1
The preparation method of lignans constituents, includes the following steps in a kind of lepidogrammitis drymoglossoides Ching：By lepidogrammitis drymoglossoides Ching pulverizing medicinal materials, 40 mesh are crossed
Sieve, it is dry at 60 DEG C that ultrasonic extraction is carried out after being 70% alcohol solution dipping 48h with volume fraction for 24 hours, extract 3 times, every time
45min, filtering merge extracting solution, are concentrated under reduced pressure to give medicinal extract；By medicinal extract first to use cotton filtering to use again after appropriate water dissolution
D101 type large pore resin absorption column chromatographic isolations, successively use water, 20%, 40%, 60%, 70%, 80%, 95% ethanol gradient elution,
70% alcohol elution is collected, then is detached through silica gel column chromatography, uses volume ratio 90 successively:1,75:1,45:1,26:1,14:1,
5:1 chloroform-methanol elution, collects 14:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg lepidogrammitis drymoglossoides Chings are extracted using 12L solvents, the 8mL water dissolutions of 1g medicinal extract.
Embodiment 2
The preparation method of lignans constituents, includes the following steps in a kind of lepidogrammitis drymoglossoides Ching：By lepidogrammitis drymoglossoides Ching pulverizing medicinal materials, 40 mesh are crossed
Sieve, the dry 23h at 55 DEG C, carries out ultrasonic extraction after being 65% alcohol solution dipping 47h with volume fraction, extract 2 times, every time
40min, filtering merge extracting solution, are concentrated under reduced pressure to give medicinal extract；By medicinal extract first to use cotton filtering to use again after appropriate water dissolution
D101 type large pore resin absorption column chromatographic isolations, successively use water, 20%, 40%, 60%, 70%, 80%, 95% ethanol gradient elution,
70% alcohol elution is collected, then is detached through silica gel column chromatography, uses volume ratio 90 successively:1,75:1,45:1,26:1,14:1,
5:1 chloroform-methanol elution, collects 14:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg lepidogrammitis drymoglossoides Chings are extracted using 11L solvents, the 6mL water dissolutions of 1g medicinal extract.
Embodiment 3
The preparation method of lignans constituents, includes the following steps in a kind of lepidogrammitis drymoglossoides Ching：By lepidogrammitis drymoglossoides Ching pulverizing medicinal materials, 40 mesh are crossed
Sieve, the dry 25h at 65 DEG C, carries out ultrasonic extraction after being 75% alcohol solution dipping 49h with volume fraction, extract 4 times, every time
50min, filtering merge extracting solution, are concentrated under reduced pressure to give medicinal extract；By medicinal extract first to use cotton filtering to use again after appropriate water dissolution
D101 type large pore resin absorption column chromatographic isolations, successively use water, 20%, 40%, 60%, 70%, 80%, 95% ethanol gradient elution,
70% alcohol elution is collected, then is detached through silica gel column chromatography, uses volume ratio 90 successively:1,75:1,45:1,26:1,14:1,
5:1 chloroform-methanol elution, collects 14:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg lepidogrammitis drymoglossoides Chings are extracted using 13L solvents, the 10mL water dissolutions of 1g medicinal extract.
Method provided by the invention can quickly obtain lignans constituents in lepidogrammitis drymoglossoides Ching, which has antibacterial, disease-resistant
Poison, antioxidant activity can be used for drug preparation.
Claims (9)
1. the preparation method of lignans constituents in a kind of lepidogrammitis drymoglossoides Ching, it is characterised in that include the following steps：By lepidogrammitis drymoglossoides Ching medicinal material
It crushes, crosses 40 mesh sieve, the dry 23-25h at 55-65 DEG C, after being 65-75% alcohol solution dippings 47-49h with volume fraction
Ultrasonic extraction is carried out, is extracted 2-4 times, each 40-50min, filtering merges extracting solution, is concentrated under reduced pressure to give medicinal extract；Medicinal extract is used
Through large pore resin absorption column after appropriate water dissolution, use successively water, 20%, 40%, 60%, 70%, 80%, 95% ethanol gradient elution,
70% alcohol elution is collected, then is detached through silica gel column chromatography, uses volume ratio 90 successively:1,75:1,45:1,26:1,14:1,
5:1 chloroform-methanol elution, collects 14:1 elution fraction, concentrates to obtain the final product.
2. preparation method according to claim 1, it is characterised in that：It is dried for 24 hours at 60 DEG C.
3. preparation method according to claim 1, it is characterised in that：It is 70% alcohol solution dipping 48h with volume fraction.
4. preparation method according to claim 1, it is characterised in that：Extraction 3 times.
5. preparation method according to claim 4, it is characterised in that：Extraction 45min every time.
6. preparation method according to claim 1, it is characterised in that：1kg lepidogrammitis drymoglossoides Chings are extracted using 11-13L solvents.
7. preparation method according to claim 1, it is characterised in that：By medicinal extract first to use cotton mistake after appropriate water dissolution
Large pore resin absorption column chromatographic isolation is used in filter again.
8. preparation method according to claim 7, it is characterised in that：The 6-10mL water dissolutions of 1g medicinal extract.
9. preparation method according to claim 7, it is characterised in that：The large pore resin absorption column is D101 types.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810821726.XA CN108743629A (en) | 2018-07-24 | 2018-07-24 | The preparation method of lignans constituents in a kind of lepidogrammitis drymoglossoides Ching |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810821726.XA CN108743629A (en) | 2018-07-24 | 2018-07-24 | The preparation method of lignans constituents in a kind of lepidogrammitis drymoglossoides Ching |
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| Publication Number | Publication Date |
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| CN108743629A true CN108743629A (en) | 2018-11-06 |
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| CN201810821726.XA Withdrawn CN108743629A (en) | 2018-07-24 | 2018-07-24 | The preparation method of lignans constituents in a kind of lepidogrammitis drymoglossoides Ching |
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| Country | Link |
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| CN (1) | CN108743629A (en) |
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2018
- 2018-07-24 CN CN201810821726.XA patent/CN108743629A/en not_active Withdrawn
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Application publication date: 20181106 |