CN108739863A - A kind of microbial pesticide and chemical pesticide compounded pesticides and its preparation method and application - Google Patents
A kind of microbial pesticide and chemical pesticide compounded pesticides and its preparation method and application Download PDFInfo
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- CN108739863A CN108739863A CN201810863384.8A CN201810863384A CN108739863A CN 108739863 A CN108739863 A CN 108739863A CN 201810863384 A CN201810863384 A CN 201810863384A CN 108739863 A CN108739863 A CN 108739863A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
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Abstract
A kind of microbial pesticide of present invention offer and chemical pesticide compounded pesticides, quality composition and ratio are：Bacillus thuringiensis 0.1% ~ 18%, Tefluthrin active compound 2.8% ~ 24%, high osmotic agent 3% ~ 17%, emulsifier 6% ~ 21%, wetting agent 2.4% ~ 6%, balance of solvent.The method for producing insecticide and application are provided simultaneously, the compounding of the two effectively increases insecticidal effect, expands the prevention and treatment range to insect pest.In addition, bacillus thuringiensis and Tefluthrin are selected suitable dosage form and auxiliary material by the present invention, it can be suitble to the dispersion and performance of bacillus thuringiensis and Tefluthrin simultaneously, effectively promote drug effect, and compared with the dosage forms such as pulvis, suspending agent, the dosage form dispersion stabilization is strong, adherence permeability is strong, easy to use, and preparation process is without dust, auxiliary agent is more environmentally friendly, is not easy to pollute to environment and people and animals.
Description
Technical field
The invention belongs to pesticide fields, and in particular to a kind of microbial pesticide and chemical pesticide compounded pesticides and its preparation
Methods and applications.
Background technology
2 committee noctuid insect, (Athetis lepigone Moschler) belong to Lepidoptera Noctuidae, be mainly distributed on Europe and
The ground such as Japan, Korea, the Siberia Far East in Asia belong to the pest of kainogenesis after new cropping system is changed in China.Adult
Like covering activity oviposition at humidity, larva is also liked covering activity and damage to crops seedling at humidity, therefore stalk is also
Field, no-tillage seeding new cropping system provide the advantageous suitable environment of large area for the outburst of 2 committee noctuid insect corn of causing harm.
2 committee noctuid insect found from 2005 in Hebei province for the first time, 2011,2 committee noctuid insect the Hebei of Huang-Huai-Hai, Henan, Shandong,
47,6 province such as Shanxi, Anhui, Along North Jiangsu county (city) of ground (city) 297, which is broken out, to be caused harm, and occurring area reaches 2,210,000 public affairs
Hectare, wherein serious about 17.3 ten thousand hectares of the plague of insects seriously threatens the production safety to Huang-Huai-Hai summer corn area of China.The worm larva
It is concentrated mainly on host crop foundation portion to cause harm, bites foundation portion's tender tissue or causes harm in stem foot moth stem, lead to death of seedling,
Especially to the corn seedling of 3 ~ 5 leaf phases, is often resulted in when serious and reseed or ruin kind of a phenomenon.Therefore, two being prevented it is urgent to provide a kind of
The method of point committee noctuid.
2 committee noctuid insect is different from most food leaf class lepidoptera pest, mainly the upper soll layer at corn aerial root
Place endangers corn root, bites cane or superficial layer root on milpa broken, and the corn field less serious case plant endangered is dilapidated,
Severe one causes the disconnected ridge that is short of seedling, while having the characteristics that the dark moist strong light of fear of happiness, generally in corn root or the soil of moistening
It survives in seam, high wheat stubble thickness wheat bran provides main living environment for 2 committee noctuid insect is big, while also preventing liquid
Reach 2 committee noctuid insect epidermis.
Since 2 committee noctuid insect causes harm summer corn seedling at present, the area of occurring and damage is gradually expanded, causing disastrous degree
It is increasingly heavier, seriously threaten the safety in production of Huang-Huai-Hai summer corn.The worm larva is concentrated mainly on host crop foundation portion
Evil bites foundation portion's tender tissue or causes harm in stem foot moth stem, leads to death of seedling, the especially corn seedling to 3 ~ 5 leaf phases,
It is often resulted in when serious and reseeds or ruin kind of a phenomenon.It prevents the worm to cause harm, the use of pesticide is one of important measures.
Dipel (Bacillus thuringiensis, abbreviation Bt) is a kind of production crystal including many mutation
The microbial source insecticide of bacillus, based on stomach poison function.The bacterium can generate two major classes toxin, i.e. endotoxin（Companion cell is brilliant
Body）And exotoxin, so that pest is stopped feeding, last pest is because of starvation and death, and exotoxin effect is slow, in husking and metamorphosis
Shi Zuoyong is apparent, the two periods are the peak periods of RNA synthesis, and exotoxin can inhibit DNA-Directed RNA polymerase.The bacterium
Effect is slow.Specificity is strong, and to nontarget organism safety, environmental risk is low, and natural enemy and beneficial organism for not killing off the insect pests etc. are excellent
Point.But the commodity bacterium also shows that certain limitations in production prevents, as quick-acting is poor, field insensitive to high instar larvae
Between the lasting period it is short and it is beneficial influenced etc. all to have become by environmental conditions such as illumination, temperature influence Bt and further successfully promote to make
Restraining factors.
Tefluthrin mainly upsets the function of nerve cell by the reciprocation with sodium-ion channel, have tag and
Fumigation action.Tefluthrin especially does harm to coleoptera, Lepidoptera and the Diptera in soil for controlling numerous soil pests
Worm has special efficacy, also is used as the repellent of coleoptera adult, therefore, has broad application prospects.But Tefluthrin is long
The single use of phase generates pest resistance to insecticide, leads to that dosage is big, cost is excessively high, brought to entire ecological environment and human health
The pollution and harm of getting worse.
Invention content
To solve the above-mentioned problems, the present invention provides a kind of microbial pesticide and chemical pesticide compounded pesticides, have compared with
High synergistic function reduces dose, reduces drug cost, reduces spray number, overcomes and delayed pest resistance to insecticide,
It is environmentally friendly.
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Bacillus thuringiensis
0.1% ~ 18%, Tefluthrin active compound 2.8% ~ 24%, high osmotic agent 3% ~ 17%, emulsifier 6% ~ 21%, wetting agent 2.4% ~ 6%, more than solvent
Amount.
Preferably, bacillus thuringiensis is with Tefluthrin mass ratio:3:8-3:28.
Preferably, it according to mass ratio is 1 that high osmotic agent, which is azone, limonene, ethylene glycol and cyanamid dimerization sodium,:0.05-0.1:
2-3:0.05-0.1 is compounded.
Preferably, emulsifier is Tween-80, sodium lignin sulfonate, fatty alcohol polyoxyethylene ether sulfate and hyperbranched poly
Carboxylic acid amide esters are according to mass ratio 1:1-2:3:1-3 is compounded.
Preferably, it is lauryl sodium sulfate, a- alkene sulfonic acid sodiums, dodecyl benzene sulfonic acid that wetting agent, which is the wetting agent,
Sodium, Negel, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, dialkyl group Succinate sodium sulfonate salt, Negel and drawing
Open one or more in powder BX, more preferably neopelex, Negel and dialkyl group succinate sulphur
The mixture of acid sodium-salt, neopelex, Negel and dialkyl group Succinate sodium sulfonate salt mass ratio
It is 1:0.5-1:1-3.
Preferably, solvent is one or more in acetone, n,N-Dimethylformamide, dimethyl sulfoxide (DMSO).
The preparation method of compounded pesticides described above takes the raw material of said ratio, will add in thuringiensis
Enter the solvent total amount 1/5, cosolvent and wetting agent, stirred 30 ~ 100 minutes with the mixing speed of 60-90 n/min；Then
Emulsifier is added while stirring thereto；Tefluthrin, stirring to Tefluthrin are added after thuringiensis dissolving
High osmotic agent is added after dissolving, adds balance solvent, mixing shakes up, and obtains transparent and homogeneous mixture preparation.
The application of compounded pesticides described above in preventing 2 committee noctuid insect.
By 1400 times of the compounded pesticides containing 3.5% bacillus thuringiensis and Tefluthrin, 1000 times, 700 times of preventions
2 committee noctuid insect.
Pesticidal combination is made in bacillus thuringiensis and Tefluthrin compounding by the present invention, and the compounding of the two effectively improves
Insecticidal effect, expands the prevention and treatment range to insect pest.In addition, breast is made in bacillus thuringiensis and Tefluthrin by the present invention
Agent, the dosage form can be suitble to the dispersion and performance of bacillus thuringiensis and Tefluthrin, effectively promote drug effect simultaneously, and
Compared with the dosage forms such as pulvis, suspending agent, the dosage form dispersion stabilization is strong, and adherence permeability is strong, easy to use, preparation process without
Dust, auxiliary agent is more environmentally friendly, is not easy to pollute to environment and people and animals.
Therefore, compounded pesticides provided by the invention have Environmental security, reduction dosage nontoxic to people and animals, reduce and use
Medicine number reduces drug cost, derives from a wealth of sources, is easy to industrialization large-scale production, is advantageous to promote.
Indoor toxicity determination test
Indoor toxicity determination test method：Due to bacillus thuringiensis and Tefluthrin be the Recompounded pesticide it is main effectively at
Point, inventor is according to the ten Along ents setting thuringiensis of summation action line in isoboles and matching for Tefluthrin
Than with reference to NY/T 1154.6-2006, test worm is handled using dip method, test worm is placed in leaching worm cage, according to dense from low to high
Degree processing dipping 10s, then sucks the upper extra liquid of polypide with filter paper, then test worm is transferred to and is placed with the plastics of fresh feed and supports
It it is 26.0 ± 1.0 DEG C in temperature, relative humidity is maintained at 70 ± 5%, the photoperiod in worm box（L/D）For the illumination cultivation of 16h/8h
It is raised in case.The death condition of 48 h, 72 h, 96 h, 120 h test worms, record total borer population and dead borer population respectively after inspection medicine, count
Calculate the death rate, LC50 and co-toxicity coefficient.
Table 1
From table 1 it follows that when thuringiensis and Tefluthrin are used in mixed way as active ingredient, two kinds of pesticides
Have complementary advantages, there is apparent synergistic effect, desinsection speed is fast, and the lasting period is long, reduces drug cost, reduces spray number, subtracts
Light toxicity, extends the old and new's kind service life.Its use with obvious effects higher than its single dose, when thuringiensis and seven fluorine chrysanthemums
The proportioning of ester is 3:28,1:6 and 3:When 8, it is 123.000,147.341 and 121.264 to spend coefficient together, and mixture effect is good.
Field efficacy evaluation test
Field control effectiveness test has synergistic effect ratio 3 according to what lab screening went out:28,1:6 and 3:8 carry out field control effectiveness test,
Effect preferable 6.2% is made（3.5%, 1.1%）Thuringiensis Tefluthrin compounded pesticides carry out field trial,
It is found in the insecticide of above-mentioned different ratio through analysis, wherein with ratio 1:6 preventive effect is best.Therefore, field is to match
1:6,3.5% 1400 times of thuringiensis Tefluthrin compounded pesticides, 1000 times, 700 times of progress field examinations are made
It tests, with 8000IU/ milligrams of 400 times of bacillus thuringiensis suspending agents, 3% Tefluthrin granule 700 medicament as a contrast again, carries out
Prevent corn 2 committee noctuid insect field control effectiveness test.
Table 2
Note：Data are to manage average data everywhere in table, and different letters indicate significant difference (P in same column<0 .05)
From Table 2, it can be seen that the control effect of compound drug is not only above the control effect of single dose, and also greatly reduce
Dosage reduces drug cost, derives from a wealth of sources, be easy to industrialization large-scale production, be advantageous to promote.Its height with obvious effects
In the use of its single dose.
In conclusion the Recompounded pesticide of prevention 2 committee noctuid insect of the present invention, using two kinds of common pesticide conducts
Main component is equipped with emulsifier and high osmotic agent, has reached mutual supplement with each other's advantages, and complementary to use, drug effect is apparent, reduce medication at
This, derives from a wealth of sources, is easy to industrialization large-scale production, be advantageous to promote, obviously excellent to the control effect of 2 committee noctuid insect
In most products currently on the market.
Specific implementation mode
It elaborates below to the embodiment of the present invention, the present embodiment is carried out lower based on the technical solution of the present invention
Implement, gives detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
For examination 2 committee noctuid insect in raising sensitive population in Shandong Academy of Agricultural Sciences's plant protection insectary, raising temperature is
（25±1）DEG C, relative humidity be（75±5）%, photoperiod L:D=16h:8h.3 instar larvae of 2 committee noctuid insect is chosen to try for standard
Worm.
Embodiment 1
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 0.6%, seven
Flumethrin 5.6%, high osmotic agent 7%, emulsifier 14%, wetting agent 3.5%, balance of solvent.
Wherein：It according to mass ratio is 1 that high osmotic agent, which is azone, limonene, ethylene glycol and cyanamid dimerization sodium,:0.05:2:0.05
It compounds.
Emulsifier is that Tween-80, sodium lignin sulfonate, fatty alcohol polyoxyethylene ether sulfate and super-branched polyesteramide are pressed
According to mass ratio 1:1:3:1.
Wetting agent is the mass ratio of neopelex, Negel and dialkyl group Succinate sodium sulfonate salt
It is 1:0.5:1.
Solvent is acetone.
Preparation method：The raw material for taking said ratio will be added the 1/5 of the solvent total amount, help in thuringiensis
Solvent and wetting agent are stirred 60 minutes with the mixing speed of 60-90 n/min；Then emulsifier is added while stirring thereto；
Tefluthrin is added after thuringiensis dissolving, stirring to Tefluthrin is added high osmotic agent after dissolving, adds surplus
Solvent, mixing shake up, and obtain transparent and homogeneous mixture preparation.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 26.754 mg/kg.
Embodiment 2
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 0.5%, seven
Flumethrin 3%, high osmotic agent 12%, emulsifier 11.5%, wetting agent 2.9%, balance of solvent.
Wherein：It according to mass ratio is 1 that high osmotic agent, which is azone, limonene, ethylene glycol and cyanamid dimerization sodium,: 0.1: 3: 0.1
It compounds.
Emulsifier is that Tween-80, sodium lignin sulfonate, fatty alcohol polyoxyethylene ether sulfate and super-branched polyesteramide are pressed
According to mass ratio 1: 2:3:3 compound.
The mass ratio of wetting agent neopelex, Negel and dialkyl group Succinate sodium sulfonate salt is
1: 1:3 compound.
Solvent is n,N-Dimethylformamide.
The preparation method is the same as that of Example 1.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 19.991 mg/kg.
Embodiment 3
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 1%, seven fluorine
Pyrethroids 4%, high osmotic agent 9%, emulsifier 17%, wetting agent 4.5%, balance of solvent.
Wherein：It according to mass ratio is 1 that high osmotic agent, which is azone, limonene, ethylene glycol and cyanamid dimerization sodium,:0.08:2.5:
0.07 compounds.
Emulsifier is that Tween-80, sodium lignin sulfonate, fatty alcohol polyoxyethylene ether sulfate and super-branched polyesteramide are pressed
According to mass ratio 1:1.5:3:2 compound.
The mass ratio of wetting agent neopelex, Negel and dialkyl group Succinate sodium sulfonate salt is
1:0.8:2 compound.
Solvent is dimethyl sulfoxide (DMSO).
The preparation method is the same as that of Example 1.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 24.143 mg/kg.
Embodiment 4
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 1.5%, seven
Flumethrin 4%, high osmotic agent 16%, emulsifier 15%, wetting agent 6%, balance of solvent.
Wherein：It according to mass ratio is 1 that high osmotic agent, which is azone, limonene, ethylene glycol and cyanamid dimerization sodium,:0.08:2.5:
0.07 compounds.
Emulsifier is that Tween-80, sodium lignin sulfonate, fatty alcohol polyoxyethylene ether sulfate and super-branched polyesteramide are pressed
According to mass ratio 1:1.5:3:2 compound.
The mass ratio of wetting agent neopelex, Negel and dialkyl group Succinate sodium sulfonate salt is
1:0.8:2 compound.
Solvent is acetone, n,N-Dimethylformamide, dimethyl sulfoxide (DMSO) according to mass ratio 1:1:1 mixes.
The preparation method is the same as that of Example 1.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 18.747 mg/kg.
Embodiment 5
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 0.5%, seven
Flumethrin 3.5%, high osmotic agent 9%, emulsifier 14%, wetting agent 3.4%, balance of solvent.
Auxiliary material and preparation method are the same as embodiment 4.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 18.934 mg/kg.
Embodiment 6
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 0.6%, seven
Flumethrin 3%, high osmotic agent 7%, emulsifier 15%, wetting agent 4.2%, balance of solvent.
Auxiliary material and preparation method are the same as embodiment 4.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 23.112 mg/kg.
Embodiment 7
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 0.5%, seven
Flumethrin 3%, high osmotic agent 12%, emulsifier 11.5%, wetting agent 2.9%, balance of solvent.
Auxiliary material and preparation method are the same as embodiment 4.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 18.051 mg/kg.
Embodiment 8
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 0.5%, seven
Flumethrin 3%, high osmotic agent 16%, emulsifier 15%, wetting agent 6%, balance of solvent.
Auxiliary material and preparation method are the same as embodiment 4.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 20.128 mg/kg.
Comparative example 1
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 0.5%, seven
Flumethrin 3%, high osmotic agent 12%, emulsifier 11.5%, wetting agent 2.9%, balance of solvent.
High osmotic agent is ethylene glycol, and emulsifier is sodium lignin sulfonate, and wetting agent is Negel, solvent N, N- bis-
Methylformamide.
The preparation method is the same as that of Example 1.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 30.128 mg/kg.
Comparative example 2
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 0.5%, seven
Flumethrin 3%, high osmotic agent 12%, emulsifier 11.5%, wetting agent 2.9%, balance of solvent.
High osmotic agent is azone, and emulsifier is Tween-80, and wetting agent is neopelex, and solvent is acetone, is prepared
Method is the same as embodiment 1.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 31.213 mg/kg.
Comparative example 3
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 0.5%, seven
Flumethrin 3%, high osmotic agent 12%, emulsifier 11.5%, wetting agent 2.9%, balance of solvent.
It according to mass ratio is 1 that high osmotic agent, which is azone, ethylene glycol,:2.5 compound.
Emulsifier is Tween-80, sodium lignin sulfonate according to mass ratio 1:1.5 compound.
Wetting agent is neopelex, Negel mass ratio is 1:0.8 compounds.
Solvent is acetone, n,N-Dimethylformamide according to mass ratio 1:1 mixes.The preparation method is the same as that of Example 1.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 28.852 mg/kg.
Comparative example 4
A kind of microbial pesticide and chemical pesticide compounded pesticides, quality composition and ratio are：Thuringiensis 0.5%, seven
Flumethrin 3%, high osmotic agent 12%, emulsifier 11.5%, wetting agent 2.9%, balance of solvent.
It according to mass ratio is 1 that high osmotic agent, which is azone, limonene, ethylene glycol,:0.08:2.5 compound.
Emulsifier is Tween-80, sodium lignin sulfonate, fatty alcohol polyoxyethylene ether sulfate according to mass ratio 1:1.5:3
It compounds.
The mass ratio of wetting agent neopelex, Negel and dialkyl group Succinate sodium sulfonate salt is
1:0.8:2 compound.
Solvent is acetone, n,N-Dimethylformamide, dimethyl sulfoxide (DMSO) according to mass ratio 1:1:1 mixes.Preparation side
Method is the same as embodiment 1.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 26.971 mg/kg.
Comparative example 5
A kind of microbial pesticide and chemical pesticide compounded pesticides, the quality composition and ratio of active constituent are：Su Yun gold brood cells
Bacillus 0.5%, Tefluthrin 3%, dosage form are wettable powder.Dispersant fatty alcohol polyoxyethylene ether sulfate 12%, wetting agent ten
Dialkyl benzene sulfonic acids sodium 11.5%, disintegrant potassium nitrate 2%, antifoaming agent organosiloxane 0.5%, filler sepiolite supply 100%.
By the agriculture chemical compounding of above-mentioned preparation, the prevention of 2 committee noctuid insect, LC50 values are used for reference to NY/T 1154.6-2006
For 41.012 mg/kg.
One, the measurement of property
In the dosage form of pesticide, auxiliary material, that is, solvent and emulsifier, wetting agent and cosolvent（High osmotic agent）Selection it is very heavy
It wants.
It measures：
(1) dispersed detection
Accurate anhydrous calcium chloride and magnesium chloride 0 .304g and 0 .139g with the crystallization water of weighing is used in the volumetric flask of 1000ml
Distilled water dissolved dilution is made into standard hard water to graduation mark.After preparation shaken well made of embodiment and comparative example, take
1ml is instilled in standard hard water and is observed deployment conditions.Syringe is placed at the above 2cm of the water surface, it is slowly one after another drop of by preparation
It instills in standard hard water, observes emulsion dispersion state.Decentralized standard such as following table.
(2) detection of stability
The hard water of the 342mg/L of room temperature is poured into the beaker of 250mL, is existed with the preparation of pipette, extract embodiment and comparative example
It is slowly stirred down and is added in hard water, be made into 100mL emulsion.After the completion, continue to stir 30s with the speed of 2~3r/s, immediately will
Emulsion moves to cleaning, in dry 100mL graduated cylinders, then graduated cylinder is placed in 25~30 DEG C of water bath with thermostatic control, stands 1h, take out
Observe emulsion.If phenomena such as can't see missible oil in graduated cylinder and layering is precipitated, emulsion stability is qualification.
(3) thermodynamic stability
Sample is put into placed 14 days in 54 ± 2 DEG C of thermostat water bath after, test tube is taken out, the water stain wiping outside test tube
It weighs after clean, the sample that quality and appearance are not apparent to variation measures active compound in interior high performance liquid chromatography (HPLC) for 24 hours
Active constituent content, the content of measurement calculates resolution ratio as a contrast before being stored up with heat, and the resolution ratio of heat storage is less than 5% and just calculates conjunction
Lattice.
Resolution ratio calculation formula：
Heat storage resolution ratio=(4) the cold storage stability of [content before (content after content-heat storage before heat storage)/heat storage] × 100%
Sample is taken to be placed in the beaker of 100ml, sample keeps whether 1h, observation appearance change, continue at 0 ± 2 DEG C at 0 DEG C
Refrigerator in place 7 days, seven days after, sample is taken out, phenomena such as observation sample is precipitated whether there is or not layering, crystallization, if having then not
Qualification, if it is also qualified to disappear in a short time, cold storage stability is good if it's not true.
Interpretation of result
The embodiment of embodiment 4-8 uses best auxiliary agent, and performance is best, it can thus be seen that performance of the auxiliary agent for dosage form
It has a major impact.
Two, drug test
The advantages of some drugs experiment is to prove drug of the present invention is set forth below.
The preparation prepared using above-described embodiment and comparative example carries out field efficacy experiment, according to effective ingredient consumption 350-
500g/hm2The big droplets spraying treatment such as ground and neighbouring weeds withered grass is carried out, or is mixed thoroughly with 75 kg/ hectares of fine sand, is made
Granule is sprinkled near plant root, with 8000IU/ milligrams of 400 times of bacillus thuringiensis suspending agents, 3% Tefluthrin particle
Medicament, development prevent corn 2 committee noctuid insect field control effectiveness test as a contrast again for agent 700.When use temperature should 18 DEG C with
On, temperature is in 30 DEG C or more best results.It from being used while allocating, is used from being diluted to, is no more than 2 h.
Preferred and auxiliary agent the preferred combination of insecticide active ingredient dosage of the present invention, the insecticides medicine of gained
Effect is best.It is 1 in mass ratio:6 fruits are best, by data it is known that two kinds of drugs of the present invention combine, improve 2 points of committee's nights
The control effect of moth reduces the usage amount and access times of drug, economical and practical, and effectively reduces ecology and environmental pollution,
And crop yield and quality can be improved.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (9)
1. a kind of microbial pesticide and chemical pesticide compounded pesticides, which is characterized in that its quality composition and ratio is：Su Yun gold buds
Spore bacillus 0.1% ~ 18%, Tefluthrin active compound 2.8% ~ 24%, high osmotic agent 3% ~ 17%, emulsifier 6% ~ 21%, wetting agent 2.4% ~ 6%,
Balance of solvent.
2. compounded pesticides according to claim 1, which is characterized in that bacillus thuringiensis and Tefluthrin mass ratio
For:3:8-3:28.
3. compounded pesticides according to claim 1, which is characterized in that high osmotic agent is azone, limonene, ethylene glycol and two
Poly cyanamid sodium is 1 according to mass ratio:0.05-0.1:2-3:0.05-0.1 is compounded.
4. compounded pesticides according to claim 1, which is characterized in that emulsifier be Tween-80, sodium lignin sulfonate,
Fatty alcohol polyoxyethylene ether sulfate is with super-branched polyesteramide according to mass ratio 1:1-2:3:1-3 is compounded.
5. compounded pesticides according to claim 1, which is characterized in that wetting agent is that the wetting agent is dodecyl sulphur
Sour sodium, a- alkene sulfonic acid sodiums, neopelex, Negel, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, dialkyl group fourth
It is one or more in two acid esters sulfonate sodiums, Negel and Nekal BX, preferably neopelex, alkane
The mixture of base sodium naphthalene sulfonate and dialkyl group Succinate sodium sulfonate salt, neopelex, Negel and two
The mass ratio of dialkyl succinylsuccinate ester sulfonate sodium is 1:0.5-1:1-3.
6. compounded pesticides according to claim 1, which is characterized in that solvent is acetone, n,N-Dimethylformamide, two
It is one or more in methyl sulfoxide.
7. the preparation method of the compounded pesticides described in a kind of one of claim 1-6, which is characterized in that take the original of said ratio
Material, by be added in thuringiensis the solvent total amount 1/5, cosolvent and wetting agent, with the stirring of 60-90 n/min
Speed stirs 30 ~ 100 minutes；Then emulsifier is added while stirring thereto；It is added after thuringiensis dissolving
Tefluthrin, stirring to Tefluthrin are added high osmotic agent after dissolving, add balance solvent, and mixing shakes up, and it is mixed to obtain transparent and homogeneous
Preparaton.
8. application of the compounded pesticides in preventing 2 committee noctuid insect described in a kind of one of claim 1-6.
9. application according to claim 8, which is characterized in that will be containing 3.5% bacillus thuringiensis and Tefluthrin
1400 times of compounded pesticides, 1000 times, 700 times of prevention 2 committee noctuid insects.
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