CN108738487A - A kind of Saline alkali tolerance identification method of industrial hemp seed germination phase - Google Patents
A kind of Saline alkali tolerance identification method of industrial hemp seed germination phase Download PDFInfo
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- CN108738487A CN108738487A CN201810412279.2A CN201810412279A CN108738487A CN 108738487 A CN108738487 A CN 108738487A CN 201810412279 A CN201810412279 A CN 201810412279A CN 108738487 A CN108738487 A CN 108738487A
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- seed germination
- industrial hemp
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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Abstract
The present invention relates to a kind of Saline alkali tolerance identification methods of industrial hemp seed germination phase, belong to crudefiber crop resistance of reverse and identify field.This method comprises the following steps：1) it chooses seeds and disinfects；2) experimental provision is arranged；3) salting liquid is handled；4) seed germination culture；5) secondary salting liquid supplement；6) establishment of parameter is identified；7) identification that various salts carry out salt tolerant, alkalinity is corresponded to according to establishment parameter.For the method for the present invention the advantage is that operation is simple and reliable, cost low velocity is fast, accuracy height as a result, comparativity are strong, can identify salt tolerant, the alkalinity of hemp simultaneously.
Description
Technical field
The present invention relates to the salt tolerant of industrial hemp seed germination phase, alkaline identification methods, belong to crudefiber crop germ plasm resource resistance of reverse
Identification, saline-alkali tolerant breeding and technical field of cultivation.
Background technology
The application of industrial hemp be related to medicine, beauty, health care, food, weaving, biological engineering material, papermaking, military supplies and its
Its light industry field, recent domestic are quickly grown.Industrial hemp is stronger to the adaptability of environment, is that waste hillside develops and uses
Preferred crop.It has been investigated that, industrial hemp also has salt-soda soil good adaptability, but different cultivars is resistance in recent years
Salt, alkali ability difference are apparent.It is high to screening resistance to there is presently no the industrial hemp salt tolerant for establishing normalization, alkaline identification method
Salt, alkaline germ plasm resource are applied to production practices and cultivate new Tolerant Cultivars, and finger of the enhancing in relation to scientific research result
It leads effect etc. and brings adverse effect.Seed in the adverse circumstances such as saline and alkaline can normal rudiment, be plant adapt to adverse circumstance, establish population
First key, therefore, salt tolerant, the method for alkalinity for establishing the correct identification seed germination phase are extremely important.
The salt tolerant of industrial hemp seed germination phase provided by the invention, alkaline identification method have no and this by literature search
Invent identical open report.
Invention content
The purpose of the present invention in the deficiency for making up industrial hemp salt tolerant, alkaline identification technology, provide a kind of operability it is strong,
The salt tolerant of the industrial hemp seed germination phase of test result accuracy, science and comparativity, alkaline identification method can be improved.
The present invention can make the salt tolerant of industrial hemp seed germination phase, alkalinity correct identification, and screening salt tolerant, alkalinity are strong
Germ plasm resource or kind for scientific researches such as Tolerance mechanisms and cultivate new salt tolerant, alkali kind or directly apply to salt-soda soil
Cultivation.Test result repeatedly shows：It is trained and is germinateed using seed disinfection, suitable concentration salt alkali process and paper, cultivated through the 7d times,
According to the relative value of germination percentage, germinating energy and plumular axis length index can to the salt tolerants of different industrial hemp variety seeds budding periods,
Alkaline power makes correct identification.
The present invention adopts the following technical scheme that realization.
A kind of Saline alkali tolerance identification method of industrial hemp seed germination phase, this method of the present invention comprise the following steps：1)
It chooses seeds and disinfects；2) experimental provision is arranged；3) salting liquid is handled；4) seed germination culture；5) secondary salting liquid supplement；6)
Identify the establishment of parameter；7) identification that various salts carry out salt tolerant, alkalinity is corresponded to according to establishment parameter；Wherein step 4) seed
Rudiment culture is cultivated specifically, the industrial hemp seed that salting liquid is handled well is placed in illumination box, preceding 3d dark training
It supports, 20 DEG C of temperature, rear 4d irradiations culture, light application time 14hd-1, 800 μm of ol.m of intensity of illumination-2.s-1Or 4000lx, temperature
25℃.Such parameter setting can reduce or eliminate seed and go mouldy, and improve percentage of seedgermination, and preventing from going mouldy reduces germination percentage and shadow
Ring the accuracy of identification.
It is further the establishment of this method step 6) identification parameter of the present invention, specially：Seed itself is reached with radicle length
It is germination standard when length, germination percentage, that is, germinating energy of 3d after statistics culture, the germination percentage of 7d, measures training after statistics culture
The plumular axis length of 7d after supporting is established with the relative value for comparing 3 parameters of calculating, the i.e. ratio of processing costs and control value
Seed germination phase salt tolerant, alkalinity identification parameter.
It is further that this method step 7) of the present invention carries out salt tolerant, alkaline according to establishing parameter and corresponds to various salts
Identification, specially：The side of body of the industrial hemp seed germination phase to neutral salt solution is identified with plumular axis length and the relative value of germinating energy
The patience for compeling i.e. salt stress is strong and weak；It is molten to basic salt with the relative value of the germinating energy and germination percentage identification industrial hemp seed germination phase
The stress of liquid is that the patience of alkaline stress is strong and weak, and index relative value shows that the patience of the kind is stronger closer to 1, on the contrary then weaker.
It is further this method step 3) salting liquid processing of the present invention, specially：On the filter paper of each culture dish uniformly
30 seeds are put, the neutral salt solution of processing addition 15mL 200-230mmol/L or the basic salt of 90-110mmol/L are molten
15mL distilled water is added in liquid, control.If if the salting liquid higher than this concentration, sprouting for industrial hemp seed will be seriously affected
Bud, if the salting liquid less than this concentration, then test effect unobvious.
Preferably, being further this method step 3) salting liquid processing of the present invention, specially：In each culture dish
30 seeds are uniformly put on filter paper, the neutral salt solution of 15mL 215mmol/L or the basic salt of 100mmol/L is added in processing
15mL distilled water is added in solution, control.
It is further that this method step 1) of the present invention is chosen seeds and disinfected, specifically, each variety selection can represent this
Varietal characteristic, mature and plump, 200, uniform seed, use 0.1%KMnO4Solution disinfection 5min, then distilled water flushing
Totally, washing time is no more than 5 minutes, finally blots the surface of the seed water with filter paper, for use.
It is further this method step 2) experimental provision setting of the present invention, specifically, the training of each kind diameter 90mm
6, ware is supported, 3 processing, 3 compare, and each culture dish spreads 3 layers of blotting paper, topmost spread 1 layer of filter paper, or 3 layers of filter paper of paving, most
Upper 1 layer of filter paper must be smooth；The secondary salting liquid supplement of step 5), specifically, replacing blotting paper, a filter paper in 3d or 4d
And culture dish, processing rejoin the above-mentioned salting liquids of 15mL, 15mL distilled water is added in control.Top layer's filter paper requires must be smooth
It puts, it is therefore an objective to up to 30 seed realizations uniformly be put, and do not influence the development taken root, germinateed in its later stage, most
Reduce to limits the adverse effect generated due to seed puts uneven, error is made to reduce as possible.
It is further the preferred NaCl solution of neutral salt solution of the present invention, the preferred Na of the alkaline salt solution2CO3
Solution.
The advantage of the invention is that：
1. a kind of salt tolerant of industrial hemp seed germination phase of offer, alkaline identification method, can improve the accurate of test result
Property, science and comparativity, be conducive to the exchange of result of study with it is shared.
2. this method operability is strong, simple and practicable, at low cost, speed is fast, moreover it is possible to while identifying industrial hemp kind
Salt tolerance and alkali resistance are strong and weak, screening salt tolerant, alkalinity strong germ plasm resource or kind, for scientific research, rearing new variety or
It cultivates in salt-soda soil.
The present invention is further explained with reference to the accompanying drawings and detailed description.
Description of the drawings
Fig. 1 is sequence of steps schematic diagram of the present invention.
Specific implementation mode
As shown in Figure 1, a kind of Saline alkali tolerance identification method of industrial hemp seed germination phase, this method of the present invention include such as
Lower step：1) it chooses seeds and disinfects；2) experimental provision is arranged；3) salting liquid is handled；4) seed germination culture；5) secondary salt is molten
Liquid supplements；6) establishment of parameter is identified；7) identification that various salts carry out salt tolerant, alkalinity is corresponded to according to establishment parameter.
It is further that this method step 1) of the present invention is chosen seeds and disinfected, specifically, each variety selection can represent this
Varietal characteristic, mature and plump, 200, uniform seed, use 0.1%KMnO4Solution disinfection 5min, then distilled water flushing
Totally, washing time is no more than 5 minutes, finally blots the surface of the seed water with filter paper, for use.
It is further this method step 2) experimental provision setting of the present invention, specifically, the training of each kind diameter 90mm
6, ware is supported, 3 processing, 3 compare, and each culture dish spreads 3 layers of blotting paper, topmost spread 1 layer of filter paper, or 3 layers of filter paper of paving, most
Upper 1 layer of filter paper must be smooth；Top layer's filter paper requires smooth to put, it is therefore an objective to which up to 30 seeds are realized uniformly pendulum
It puts, and does not influence the development taken root, germinateed in its later stage, reduce generated not due to seed puts uneven to the maximum extent
Profit influences, and error is made to reduce as possible.
It is further this method step 3) salting liquid processing of the present invention, specially：On the filter paper of each culture dish uniformly
30 seeds are put, the neutral salt solution of 15mL 200-230mmol/L is added (first with 0,50,100,150,200 and in processing
250mmol/L is tested, and it is preferable to filter out 200mmol/L, then refine concentration with 200mmol/L, 205mmol/L, 210mmol/L,
215mmol/L, 220mmol/L, 225mmol/L, 230mmol/L are tested, and are obtained more accurate suitable for neutral salinity) or
90-110mmol/L basic salt (first with 0,30,60,90,120,150mmol/L test, it is preferable to filter out 90-120mmol/L,
Concentration is refined again to be tried with 90mmol/L, 95mmol/L, 100mmol/L, 105mmol/L, 110mmol/L, 115mmol/L
Test, obtain more accurate appropriate alkaline salinity) solution, control addition 15mL distilled water.If being higher than the salting liquid of this concentration
Words, will seriously affect the rudiment of industrial hemp seed, and if the salting liquid less than this concentration, then test effect is undesirable.
Preferably, being further this method step 3) salting liquid processing of the present invention, specially：In each culture dish
30 seeds are uniformly put on filter paper, the neutral salt solution of 15mL 215mmol/L or the basic salt of 100mmol/L is added in processing
15mL distilled water is added in solution, control.
It is further this method step 4) seed germination culture of the present invention, specifically, the industry that salting liquid is handled well is big
Numb seed, which is placed in illumination box, to be cultivated, preceding 3d dark culturings, 20 DEG C of temperature, rear 4d irradiations culture, light application time 14hd-1, 800 μm of ol.m of intensity of illumination-2.s-1Or 4000lx, 25 DEG C of temperature.Such parameter setting can reduce or eliminate seed and go mouldy,
Percentage of seedgermination is improved, preventing from going mouldy reduces germination percentage and influence the accuracy identified.
It is further the secondary salting liquid supplement of this method step 5) of the present invention, specifically, being replaced in 3d or 4d primary
Blotting paper, filter paper and culture dish, processing rejoin the above-mentioned salting liquids of 15mL, and 15mL distilled water is added in control.
It is further the establishment of this method step 6) identification parameter of the present invention, specially：Seed itself is reached with radicle length
It is germination standard when length, germination percentage, that is, germinating energy of 3d after statistics culture, the germination percentage of 7d, measures training after statistics culture
The plumular axis length of 7d after supporting is established with the relative value for comparing 3 parameters of calculating, the i.e. ratio of processing costs and control value
Seed germination phase salt tolerant, alkalinity identification parameter.
It is further that this method step 7) of the present invention carries out salt tolerant, alkaline according to establishing parameter and corresponds to various salts
Identification, specially：Identify the industrial hemp seed germination phase to neutral salt stress, that is, salt with plumular axis length and the relative value of germinating energy
The patience of stress is strong and weak；Identify the industrial hemp seed germination phase to Alkaline salt stress, that is, alkali with the relative value of germinating energy and germination percentage
The patience of stress is strong and weak, and index relative value shows that the patience of the kind is stronger closer to 1, on the contrary then weaker.
It is further the preferred NaCl solution of neutral salt solution of the present invention, the preferred Na of the alkaline salt solution2CO3
Solution.
It is preferred that the test data of solution concentration：
Parameter one：Germination percentage, that is, germinating energy (%) of 3d after culture
Parameter two：The germination percentage (%) of 7d after culture
Parameter three：The plumular axis length (cm) of 7d after culture
This method is used for the salt tolerant of domestic key industry Hemp Varieties by inventor, alkalinity is identified.By to multi-parameter
It is determined after combinatory analysis, is to neutral salt stress with plumular axis length and the relative value of germinating energy identification industrial hemp seed germination phase
The patience of salt stress is strong and weak, identifies that the industrial hemp seed germination phase compels i.e. alkali to basic salt with the relative value of germinating energy and germination percentage
The patience of stress is strong and weak.It was verified that operation is simple and reliable, at low cost, speed is fast for this method, the accurate of test result can be improved
Property, science and comparativity, be conducive to the exchange of result of study with it is shared；Can identify simultaneously industrial hemp kind salt tolerance and
Alkali resistance is strong and weak, and screening salt tolerant, alkalinity strong germ plasm resource or kind are planted for scientific research, rearing new variety or salt-soda soil
Training.
Above-described is only specific embodiments of the present invention, and the common sense such as well known concrete structure and characteristic are herein in scheme
It does not describe excessively.It should be pointed out that for those skilled in the art, without departing from the structure of the invention, also
Several modifications and improvements can be made, these should also be considered as protection scope of the present invention, these all do not interfere with of the invention real
The effect and patent practicability applied.The scope of protection required by this application should be based on the content of the claims, specification
In the records such as specific implementation mode can be used for explaining the content of claim.
Claims (8)
1. a kind of Saline alkali tolerance identification method of industrial hemp seed germination phase, which is characterized in that this method comprises the following steps：
1) it chooses seeds and disinfects；2) experimental provision is arranged；3) salting liquid is handled；4) seed germination culture；5) secondary salting liquid
Supplement；6) establishment of parameter is identified；7) identification that various salts carry out salt tolerant, alkalinity is corresponded to according to establishment parameter；Wherein walk
Rapid 4) seed germination culture is cultivated specifically, the industrial hemp seed of salting liquid processing is placed in illumination box, and preceding 3d is black
Light culture, 20 DEG C of temperature, rear 4d irradiations culture, light application time 14hd-1, 800 μm of ol.m of intensity of illumination-2.s-1Or 4000lx,
25 DEG C of temperature.
2. the Saline alkali tolerance identification method of industrial hemp seed germination phase according to claim 1 a kind of, which is characterized in that
This method step 6) identifies the establishment of parameter, specially：As germination standard when reaching this body length of seed using radicle length, statistics training
Germination percentage, that is, germinating energy of 3d after supporting, the germination percentage of 7d after statistics culture, measures the plumular axis length of 7d after culture, and right
According to the relative value for calculating 3 parameters, the i.e. ratio of processing costs and control value is compared, seed germination phase salt tolerant, alkalinity identification are established
Parameter.
3. the Saline alkali tolerance identification method of industrial hemp seed germination phase according to claim 2 a kind of, which is characterized in that
This method step 7) corresponds to the identification that various salts carry out salt tolerant, alkalinity according to parameter is established, specially：With plumular axis length and
The relative value identification industrial hemp seed germination phase of germinating energy is to the patience power that the stress of neutral salt solution is salt stress；With hair
Bud gesture and the relative value of germination percentage identification industrial hemp seed germination phase are strong to the patience of the i.e. alkaline stress of stress of alkaline salt solution
Weak, index relative value shows that the patience of the kind is stronger closer to 1, on the contrary then weaker.
4. the Saline alkali tolerance identification method of industrial hemp seed germination phase according to claim 1 a kind of, which is characterized in that
The processing of this method step 3) salting liquid, specially：30 seeds are uniformly put on the filter paper of each culture dish, processing is added
15mL distilled water is added in the neutral salt solution of 15mL 200-230mmol/L or the alkaline salt solution of 90-110mmol/L, control.
5. the Saline alkali tolerance identification method of industrial hemp seed germination phase according to claim 1 a kind of, which is characterized in that
The processing of this method step 3) salting liquid, specially：30 seeds are uniformly put on the filter paper of each culture dish, processing is added
15mL distilled water is added in the neutral salt solution of 15mL215mmol/L or the alkaline salt solution of 100mmol/L, control.
6. the Saline alkali tolerance identification method of industrial hemp seed germination phase according to claim 1 a kind of, which is characterized in that
This method step 1) is chosen seeds and is disinfected, specifically, each variety selection can represent the varietal characteristic, mature and plump, uniformly
200, consistent seed, uses 0.1%KMnO4Solution disinfection 5min, then distilled water flushing is clean, and washing time is no more than 5 points
Clock finally blots the surface of the seed water, for use with filter paper.
7. the Saline alkali tolerance identification method of industrial hemp seed germination phase according to claim 1 a kind of, which is characterized in that
This method step 2) experimental provision is arranged, specifically, 6, the culture dish of each kind diameter 90mm, 3 processing, 3 right
According to each culture dish spreads 3 layers of blotting paper, topmost spreads 1 layer of filter paper, or 3 layers of filter paper of paving, most upper 1 layer of filter paper must be smooth；Step
5) secondary salting liquid supplement, rejoins specifically, replacing a blotting paper, filter paper and culture dish, processing in 3d or 4d
15mL distilled water is added in the above-mentioned salting liquids of 15mL, control.
8. according to the Saline alkali tolerance identification method of any industrial hemp seed germination phase of claim 3 or 4 or 5 a kind of,
It is characterized in that, the preferred NaCl solution of the neutral salt solution, the preferred Na of the alkaline salt solution2CO3Solution.
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| Application Number | Priority Date | Filing Date | Title |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771702A (en) * | 2020-07-27 | 2020-10-16 | 黑龙江省科学院大庆分院 | Method for evaluating saline-alkali resistance of industrial hemp in seedling stage by using water culture method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4905411A (en) * | 1985-05-16 | 1990-03-06 | National Research Development Corporation | Seed treatment |
-
2018
- 2018-05-03 CN CN201810412279.2A patent/CN108738487A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4905411A (en) * | 1985-05-16 | 1990-03-06 | National Research Development Corporation | Seed treatment |
| US4905411B1 (en) * | 1985-05-16 | 2000-05-02 | British Tech Group | Seed treatment |
Non-Patent Citations (2)
| Title |
|---|
| 程霞等: "大麻种子在NaCl 单盐处理下的萌发特性", 《江西农业学报F》 * |
| 胡华冉等: "不同盐碱胁迫对大麻种子萌发和幼苗生长的影响", 《植物资源与环境学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771702A (en) * | 2020-07-27 | 2020-10-16 | 黑龙江省科学院大庆分院 | Method for evaluating saline-alkali resistance of industrial hemp in seedling stage by using water culture method |
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Application publication date: 20181106 |