CN108732168A - A kind of light source is from constant photometer - Google Patents

A kind of light source is from constant photometer Download PDF

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Publication number
CN108732168A
CN108732168A CN201710274067.8A CN201710274067A CN108732168A CN 108732168 A CN108732168 A CN 108732168A CN 201710274067 A CN201710274067 A CN 201710274067A CN 108732168 A CN108732168 A CN 108732168A
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CN
China
Prior art keywords
light source
photometer
constant
sample
standard
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Pending
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CN201710274067.8A
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Inventor
陈爱美
王在彬
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Individual
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Individual
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Priority to CN201710274067.8A priority Critical patent/CN108732168A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02Details
    • G01J3/10Arrangements of light sources specially adapted for spectrometry or colorimetry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106General arrangement of respective parts
    • G01N2021/0112Apparatus in one mechanical, optical or electronic block
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00Features of devices classified in G01N21/00
    • G01N2201/06Illumination; Optics
    • G01N2201/063Illuminating optical parts

Abstract

The present invention is a kind of light source from constant photometer, including light source module, colorimetric room, photoelectric detector and controller, can also configure the parts such as touch display, remote communication interface, keyboard.Light source module has light source (21), loophole (22), sidelight hole (23), photoelectric detector (24), electric current automatic regulating system (25) and convex lens (26).Controller adjusts the operating current of light source (21) according to the output signal of photoelectric detector (24)(Or voltage), keep the constant of light source (21) light emission luminance.Controller goes out standard curve according to the measurement result automatic Fitting of two standard samples, and calculates the concentration value of sample to be tested automatically according to the measurement result of sample to be tested and standard curve.Light source has human-computer interaction interface and teletransmission communication function from constant photometer.

Description

A kind of light source is from constant photometer
Technical field
The present invention relates to a kind of spectrophotometer of analytical chemistry field, especially a kind of light source, can from constant photometer So that the bright intensity for being maintained at some setting of the photometric light source automatic steady.Spectrophotometer of the present invention Detection wavelength range cover the wave bands such as extreme ultraviolet light, ultraviolet light, visible light, infrared light and far red light.
Background technology
Spectrophotometer is exactly the instrument for carrying out quantitative and qualitative analysis to substance using spectrophotometry.And spectrophotometry Be then by measure measured matter light in certain wave strong point or a wavelength range trap, to the substance carry out it is qualitative and Quantitative analysis.
A branch of collimated monochromatic ligth(Io)When by coloured clear solution, a part of light can penetrate solution(It), another Part is absorbed by solution(Ia), some is reflected by vessel surface(Ir), then have (formula 1):Io=It+Ia+Ir.So, The solution light transmittance is (formula 2): T = It/Io.According to Lambert-Beer laws (formula 3):A=lg(Io/It)=lg (1/T)=K*b*c, wherein A is absorbance, and b is liquid layer thickness, and c is strength of fluid, and K is constant.I.e.:When lambda1-wavelength, One timing of property and temperature of absorbing solutions, the absorbance A of liquid are directly proportional to the thickness b of liquid, the concentration c of liquid.
Substance is to the selective absorbing wavelength of light, and corresponding absorption coefficient is the physical constant of the substance.When known After the absorption coefficient of certain pure material under certain condition, its trap can be measured with similarity condition by the test sample wiring solution-forming, The content of the substance in test sample can be calculated by above formula.
Due to the factors such as brightness fluctuation of photometric monochromatic optical purity, light source, to obtain accurate measurement result, In operating process, need that photometer " adjust the operations such as zero " and " adjust T=100% ", and prepare, detect three groups or more Standard items carry out the drafting of standard curve according to measurement result.Then the absorbance of sample to be tested is measured, and according to sample to be tested Absorbance and standard curve calculate the concentration of sample to be tested.
Invention content
Photometer disclosed by the invention can automatically adjust the brightness of light source, keep the brightness of photometer light source constant always Ground is maintained at some suitable value.
Since photometric light source according to the present invention is constant, so in measurement process, need not carry out " adjusting zero The operations such as point " and " adjusting T=100% ", simplify chemical examination operating procedure.
Photometer according to the present invention has the function of curve automatic Calibration, it is only necessary to measure the mark of two various concentrations Sample, so that it may which standard curve is gone out with automatic Calibration.
Photometer according to the present invention includes mainly light source module, colorimetric room, photoelectric detector and controller, also may be used To configure the accessories such as touch display, remote communication interface, keyboard.
Photometer according to the present invention, light source die illuminator in the block can be white light sources, then pass through optical filtering The light-dividing devices such as mirror obtain monochromatic light;The LED light that multiple and different wavelength can also be used, according to the characteristic absorption of substances to be measured Wavelength selectes the LED light of corresponding wavelength, is measured to different substances to realize.
Photometer according to the present invention includes mainly light source module, colorimetric room, photoelectric detector and controller, also may be used To configure the accessories such as touch display, remote communication interface, keyboard.Fig. 1 is photometric structure according to the present invention Schematic diagram.
Photometric light source module according to the present invention includes light source(LED light or other light sources), photoelectric detector, electricity Stream(Or voltage)Automatic regulating system.The structural schematic diagram of light source module is Fig. 2:In Fig. 2,21 be LED light, and 22 be loophole, The material of loophole is the plastics etc. of glass or high-transmittance, realizes to be purpose that light transmission seals again, prevents dust from entering light source Inside modules.23 be sidelight hole, and 24 be photoelectric detector.θ angles in figure indicate central axes and the sidelight hole 23 of loophole 22 Central axes between angle.By adjusting sidelight hole 23 or the size of θ angles, it can adjust and be irradiated on photoelectric detector 24 The intensity of the light in face.If the intensity for the light being irradiated to above photoelectric detector 24 is too big, the old of photoelectric detector 24 can be accelerated Change, reduces the service life;If the intensity for the light being irradiated to above photoelectric detector 24 is too small, accuracy of detection can be reduced.25 are Electric current(Or voltage)Automatic regulating system, major function are to adjust light source according to the power of the electric signal of photoelectric detector 24 Operating current(Or voltage), light source module is made to send out the light of brightness constancy.26 be convex lens, and effect is to become the light of light transmission A branch of directional light.
Electric current(Or voltage)Automatic regulating system can configure individual operation control chip;It can not also configure individually Operation control chip, but electric current is completed by controller(Or voltage)The logical operation control function of automatic regulating system.
Photometer according to the present invention after powering, automatically adjusts the operating current of light source(Or voltage), in light source die After the brightness stability for the light that block is sent out, instrument prompt " enters working condition ".
By showing menu or function button, select into " calibration curve " state.
According to the requirement that instrument prompts, the concentration of first standard sample, the concentration c of first standard sample are inputted1 It indicates.Then, the cuvette equipped with first standard sample is put into photometric colorimetric room, closes the window like color room After, it selectes and confirms that button, photometer automatically determine out the size of the electric signal of photoelectric detector 2, wait for the electricity of photoelectric detector 2 After signal stabilization, controller automatically records the size of electric signal at this time, which is x1
According to the requirement that instrument prompts, the concentration of second standard sample, the concentration c of second standard sample are inputted2 It indicates.Then, the cuvette equipped with second standard sample is put into photometric colorimetric room, closes the window like color room After, it selectes and confirms that button, photometer automatically determine out the size of the electric signal of photoelectric detector 2, wait for the electricity of photoelectric detector 2 After signal stabilization, controller automatically records the size of electric signal at this time, which is x2
Controller according to(c1, x1)With(c2, x2)This two groups of data, automatic Fitting go out the function formula of standard curve (formula 4):c=m*lg(x)+n.In the functional expression, c indicates that the concentration of solution, x indicate the electricity of the photodetector 2 corresponding to solution Signal, m and n are constant.
The function formula (formula 4) of standard curve:c=m* lg(x) derivation of+n is as follows:
When measuring first standard sample, a branch of collimated monochromatic ligth(Io)By first standard sample, a part of light can To penetrate solution(It1), another part absorbed by solution(Ia1), some is reflected by vessel surface(Ir1), then have (formula 1):Io=It1+Ia1+Ir1.The intensity I of light sourceoIt is steady state value, then, which is (formula 5): T1= It1/Io 。 According to Lambert-Beer laws (formula 3), can export (formula 6):A1=lg(Io/It1)=lg(1/T1)= K*b*c1, In, A1For absorbance, b is liquid layer thickness, c1For strength of fluid, K1For constant.It is irradiated to the strong of the light above photoelectric detector 2 Degree is Kt*It1, KtFor percentage coefficient, the K of the light of light transmission cuvette is indicatedt% is irradiated to above photoelectric detector 2.Then, electro-detection The electric signal x of device 21Relationship with light transmittance is (formula 7):x1=Kg* Kt*It1, K in formulagIndicate the photoelectricity of photodetector 2 Transfer efficiency,.It is derived (formula 8) by (formula 6) and (formula 7):lg(Kg*Kt/x1)= K*b*c1
When measuring second standard sample, similarly, can derive (formula 9):lg(Kg*Kt/x2)= K*b*c2
(formula 8) is utilized to derive (formula 10):c1= [1/(K*b)]* lg(Kg* Kt / x1)= [1/(K*b)]*[lg (Kg* Kt)- lg(x1)]= [1/(K*b)]*lg(Kg* Kt)- [1/(K*b)]* lg(x1) 。
(formula 9) is utilized to derive (formula 11):c2= [1/(K*b)]*lg(Kg* Kt)- [1/(K*b)]* lg(x1) 。
(formula 10) and (formula 11) enables ([1/ (K*b)] * lg(Kg* Kt))=n, enable (- [1/ (K*b)])=m.It is then (public Formula 10) and (formula 11) c can be reduced to1=m*lg(x1)+n and c2=m*lg(x2)+n .In formula, m and n only with environment temperature, Device characteristics are related and the concentration of solution is unrelated.When temperature is constant and the intensity of light source is constant, (formula 10) and (formula 11) is It sets up.
Controller according to(c1, x1)With(c2, x2)This two groups of data are utilized (formula 10) and (formula 11), can be calculated Go out m and n.m=(c1- c1)/[ lg(x1)- lg(x2)] ,n= c1 - lg(x1)*(c1- c1)/ [ lg(x1)- lg(x2)] 。
Controller automatic Fitting goes out the function formula (formula 12) of standard curve:c=m*lg(x)+n= lg(x)* (c1- c1)/[ lg(x1)- lg(x2)]+ c1 - lg(x1)*(c1- c1)/ [ lg(x1)- lg(x2)] 。
After controller fits the function formula of standard curve, photometer prompt can carry out test job.It will be equipped with The cuvette of sample to be tested color solution is put into colorimetric room, is then clicked " confirming button ".Controller is according to sample to be tested colorimetric The electric signal x of photodetector 2 corresponding to liquid utilizes the function formula (formula 4) of standard curve:c=m*lg(x)+n, it is automatic to count The concentration of sample to be tested is calculated, and exports measurement result.
Description of the drawings
A kind of Fig. 1 light sources according to the present invention are from constant photometric structural schematic diagram.In Fig. 1,1 is light source module, 2 It is photoelectric detector, 3 be cuvette, and 4 be colorimetric room, and 5 be controller, and 6 be touch display, and 7 be remote communication interface, and 8 are Keyboard.
Fig. 2 is the structural schematic diagram of light source module.In Fig. 2,21 be light source, and 22 be loophole, and 23 be sidelight hole, and 24 be light Photodetector, 25 be electric current(Or voltage)Automatic regulating system.θ angles in figure indicate central axes and the sidelight hole of loophole 22 Angle between 23 central axes.26 be convex lens, and effect is that the light of light transmission is become a branch of directional light.
Specific implementation mode
Specific implementation mode 1:Light is divided using silicon molybdenum blue to the content of silicon in boiler feed water according to GB/T 12149-2007 Degree method is analyzed.
The LED light of wavelength 640nm is selected in light source module(If including the LED light of multiple and different wavelength in light source module, Only open the LED of wavelength 640nm).The rated operational current of LED light is Ie.Electric current(Or voltage)The electric current of automatic regulating system Output area is from 0 to Ie, and the size of output current is adjusted by controller.Electric current(Or voltage)The output of automatic regulating system Electric current is exactly the operating current of LED light.
Controller detects that the electric signal size of photoelectric detector 24 is U.The factory setting value of the signal is Uset
Light source is from after the booting of constant photometer, controller setting electric current(Or voltage)The electric current of automatic regulating system is initially 0.75* Ie(General any one current value that may be set between 0.5* Ie and 0.9* Ie).Controller detects that photoelectricity is examined The electric signal size for surveying device 24 is U.In each program cycle period of controller, a U value is all detected, and by U and UsetInto Row size compares, if U>Uset, controller reduces electric current automatically(Or voltage)The output current of automatic regulating system(Or electricity Pressure);If U<Uset, controller increases electric current automatically(Or voltage)The output current of automatic regulating system(Or voltage).Make luminosity During counting work, U is consistently equal to Uset, realize light source from constant function.
When light source module enters light source from after steady state, i.e., U is consistently equal to UsetAfter, photometer enters work shape State.
Standard curve staking-out work is carried out, it, can be without if being stored with suitable standard curve in photometer Standard curve is demarcated, and directly carries out the measurement of sample to be tested.
According to related request in GB/T 12149-2007, two parts of silica standard color solutions, this two portions of titers are configured The concentration of silica be respectively c1And c2
Into photometric calibration curve menu.
According to the requirement that instrument prompts, the concentration c of first standard sample is inputted1Value.Then, first standard will be housed The cuvette of sample is put into photometric colorimetric room, is closed after the window like color room, is selected and is confirmed button, and photometer is certainly The size of the dynamic electric signal for determining photoelectric detector 2, after the electric signal of photoelectric detector 2 is stablized, controller automatically records The size of electric signal at this time, the electric signal numerical value are x1
According to the requirement that instrument prompts, the concentration c of second standard sample is inputted2Value.Then, second standard will be housed The cuvette of sample is put into photometric colorimetric room, is closed after the window like color room, is selected and is confirmed button, and photometer is certainly The size of the dynamic electric signal for determining photoelectric detector 2, after the electric signal of photoelectric detector 2 is stablized, controller automatically records The size of electric signal at this time, the electric signal numerical value are x2
Controller according to(c1, x1)With(c2, x2)This two groups of data, automatic Fitting go out the function formula of standard curve (formula 4):c=m*lg(x) in+n m and n numerical value.m=(c1- c1)/[ lg(x1)- lg(x2)] ,n= c1 - lg(x1)*(c1- c1)/ [ lg(x1)- lg(x2)] .Standard curve staking-out work terminates, and photometer exits standard curve calibration menu.
Photometer enters measurement menu.
Cuvette equipped with sample to be tested color solution is put into colorimetric room, is then clicked " confirming button ".Controller root According to the electric signal x of the photodetector 2 corresponding to sample to be tested color solution, the function formula (formula 4) of standard curve is utilized:c=m* lg(x)+n, calculates the concentration of sample to be tested automatically, and exports measurement result.
Specific implementation mode 2:According to GB/T 6437-2002 to total phosphorus yield in feed
Light source selects hydrogen lamp in light source module.And it is the optical filtering of 400nm to select wavelength.
Controller detects that the electric signal size of photoelectric detector 24 is U.The factory setting value of the signal is Uset
Light source is from after the booting of constant photometer, controller setting electric current(Or voltage)The initial voltage of automatic regulating system is 0.9 times or so of hydrogen lamp rated operational voltage.Controller detects that the electric signal size of photoelectric detector 24 is U.In controller Each program cycle period in, all detect a U value, and by U and UsetSize comparison is carried out, if U>Uset, controller is certainly It is dynamic to reduce electric current(Or voltage)The output current of automatic regulating system(Or voltage);If U<Uset, controller increases electric current automatically (Or voltage)The output current of automatic regulating system(Or voltage).During so that photometer is worked, U is consistently equal to Uset, realize light source From constant function.
When light source module enters light source from after steady state, i.e., U is consistently equal to UsetAfter, photometer enters work shape State.
Standard curve staking-out work is carried out, it, can be without if being stored with suitable standard curve in photometer Standard curve is demarcated, and directly carries out the measurement of sample to be tested.
According to related request in GB/T 6437-2002, two parts of phosphorus standard color solutions are configured, the phosphorus of this two portions of titers Concentration is respectively c1And c2
Into photometric calibration curve menu.
According to the requirement that instrument prompts, the concentration c of first standard sample is inputted1Value.Then, first standard will be housed The cuvette of sample is put into photometric colorimetric room, is closed after the window like color room, is selected and is confirmed button, and photometer is certainly The size of the dynamic electric signal for determining photoelectric detector 2, after the electric signal of photoelectric detector 2 is stablized, controller automatically records The size of electric signal at this time, the electric signal numerical value are x1
According to the requirement that instrument prompts, the concentration c of second standard sample is inputted2Value.Then, second standard will be housed The cuvette of sample is put into photometric colorimetric room, is closed after the window like color room, is selected and is confirmed button, and photometer is certainly The size of the dynamic electric signal for determining photoelectric detector 2, after the electric signal of photoelectric detector 2 is stablized, controller automatically records The size of electric signal at this time, the electric signal numerical value are x2
Controller according to(c1, x1)With(c2, x2)This two groups of data, automatic Fitting go out the function formula of standard curve (formula 4):c=m*lg(x) in+n m and n numerical value.m=(c1- c1)/[ lg(x1)- lg(x2)] ,n= c1 - lg(x1)*(c1- c1)/ [ lg(x1)- lg(x2)] .Standard curve staking-out work terminates, and photometer exits standard curve calibration menu.
Photometer enters measurement menu.
Cuvette equipped with sample to be tested color solution is put into colorimetric room, is then clicked " confirming button ".Controller root According to the electric signal x of the photodetector 2 corresponding to sample to be tested color solution, the function formula (formula 4) of standard curve is utilized:c=m* lg(x)+n, calculates the concentration of sample to be tested automatically, and exports measurement result.
The technical means disclosed in the embodiments of the present invention is not limited to the technical means disclosed in the above technical means, and further includes By the above technical characteristic arbitrarily the formed technical solution of combination.The above is the specific implementation mode of the present invention, should be referred to Go out, for those skilled in the art, without departing from the principle of the present invention, can also make several Improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.

Claims (6)

1. a kind of light source is from constant photometer, including:
A) light source module, can automatically adjust the brightness of monochromatic source, and automatically keep the brightness constancy of light source;
B) colorimetric room;
C) controller;
D) photoelectric detector;
E) touch display;
F) remote communication interface;
G) keyboard.
2. light source according to claim 1 is from constant photometer, which is characterized in that
Above-mentioned light source module can automatically keep the constant of light-source brightness, can eliminate shadow of the light-source brightness fluctuation to measurement result It rings;
Above-mentioned light source module can feel that the characteristic absorption wavelength of substances to be measured carrys out the automatic or manual corresponding operating wave of replacement It is long;
Above controller can store photometric manufacture parameter, the function expression of automatic storage standard curve, automatic storage The measurement result of each standard specimen or sample;
Above controller has the function of the mathematical operations such as logarithm operation.
3. light source according to claim 1 is from constant photometer, which is characterized in that
Standard curve can be gone out according to the measurement result and standard sample concentration input value, automatic Fitting of two standard samples, and Can be according to the measured value and standard curve to sample to be tested, the automatic concentration value for exporting sample to be tested.
4. light source according to claim 1 is from constant photometer, which is characterized in that
Measurement result can be transported to upper layer device by remote communication interface(Host computer)In or receive upper layer device(Host computer) Instruction.
5. light source according to claim 1 is from constant photometer, which is characterized in that
Light source module keeps the brightness for the light that light source sends out permanent by automatically adjusting the size of the operating current (or voltage) of light source It is fixed.
6. light source according to claim 1 is from constant photometer, which is characterized in that
A kind of approximating method of standard curve, it is only necessary to two standard samples of various concentration, so that it may which standard is gone out with automatic Fitting Curve.
CN201710274067.8A 2017-04-25 2017-04-25 A kind of light source is from constant photometer Pending CN108732168A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710274067.8A CN108732168A (en) 2017-04-25 2017-04-25 A kind of light source is from constant photometer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710274067.8A CN108732168A (en) 2017-04-25 2017-04-25 A kind of light source is from constant photometer

Publications (1)

Publication Number Publication Date
CN108732168A true CN108732168A (en) 2018-11-02

Family

ID=63934132

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710274067.8A Pending CN108732168A (en) 2017-04-25 2017-04-25 A kind of light source is from constant photometer

Country Status (1)

Country Link
CN (1) CN108732168A (en)

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Application publication date: 20181102