CN108728574A - Main effect QTL site, SNP marker and the application of cabbage type rape seed density character - Google Patents
Main effect QTL site, SNP marker and the application of cabbage type rape seed density character Download PDFInfo
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- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
The present invention provides a kind of main effect QTL site of cabbage type rape seed density character, is located at the 27888132nd bit base~28240668th of the A09 chromosomes of cabbage type rape between bit base.Preferably, being 30.45% to the contribution rate of cabbage type rape seed density character.With the first SNP marker close linkage, the first SNP marker is located at the 27888132nd bit base, is A or G, which leads to polymorphism.With the second SNP marker close linkage, the second SNP marker is located at the 28240668th bit base, is A or T, which leads to polymorphism.With peak value SNP marker close linkage, peak value SNP marker is located at the 28151624th bit base, is T or G, which leads to polymorphism.Additionally provide relevant SNP marker and application.The main effect QTL site of the cabbage type rape seed density character of the present invention is high to the contribution rate of cabbage type rape seed density character, key effect is played to the regulation and control of cabbage type rape seed density, it can be used as map based cloning and molecular marker assisted selection, be suitable for large-scale promotion application.
Description
Technical field
The present invention relates to molecular biology and rapeseed breeding technical fields, more particularly to cabbage type rape seed density character
Technical field, in particular to a kind of main effect QTL site, SNP marker and the application of cabbage type rape seed density character.
Background technology
Rape is subordinate to Cruciferae (Cruciferae) Btassica (Brassica), is that an important excellent oil plant is made
Object.According to botany and biological property, rape can be divided into three main Types：Turnip type rape (Brassica
campestdris,L.；2n=20, aa), mustard type rape (Brassica juncea, Coss.；2n=36, aabb) and wild cabbage
Type rape (Brassica napus, L.；2n=38, aacc).Cabbage type rape is now to be especially Asia, Europe and north in the world
One of most important oil crops in America area and the first big oil crops of China, rapeseed oil account for entire domestic food plant
55% or more of oil.Recently as the continuous rising of the price of labour power and production cost, under China's Rape-seed production cultivated area
Drop and vegetable seed is short, corresponding rapeseed import volume is continuously increased (http://m.cofeed.com/,2018).In face of China's rape
Short severe situation is supplied, it is the 4th time indicated that China rapeseed breeding man, which proposes with " three high " (high yield, height are oily, efficient),
Rape industry is developed by leaps and bounds the target (historical review and prospect of Wang Hanzhong (2010) China development of rape industry.Chinese oil plant is made
Object journal 32 (2):300-302).The cultivation of wherein high yield new rape variety is one of the main task of the following rapeseed breeding, must
Important guarantee will be provided safely to the development of China's Rape industry and oil plant.
Siliqua of oilseed rape is directly related to the accumulation of photosynthate, influences rape as important " library " and " source " organ
Yield.Single plant silique number and per fruit grain number be yield of rape inscape, in silique density and silique seed raw density will
Single plant silique number is influenced respectively and per fruit grain number, having directly or indirectly on yield of rape influences.Therefore, increase silique density
Raw density with seed in silique becomes the possible approaches of raising yield of rape, to the research of itself and correlated traits in rape heredity
Have great importance in breeding.
Accordingly, it is desirable to provide a kind of main effect QTL site of cabbage type rape seed density character, to cabbage type rape seed
The contribution rate of granule density character is high, and key effect is played to the regulation and control of cabbage type rape seed density, can be used as map based cloning and
Molecular marker assisted selection.
Invention content
In order to overcome the disadvantages of the prior art mentioned above, it is an object of the present invention to provide a kind of cabbage type rape seeds
The main effect QTL site of granule density character, it is high to the contribution rate of cabbage type rape seed density character, to cabbage type rape seed
The regulation and control of density play key effect, can be used as map based cloning and molecular marker assisted selection, are suitable for large-scale promotion application.
Another object of the present invention is to provide a kind of SNP in the main effect QTL site of cabbage type rape seed density character
Molecular labeling can detect the size of cabbage type rape seed density, can predict the size of cabbage type rape seed density,
The size of cabbage type rape seed density can effectively be selected, can be also used for the big cabbage type rape of seed density
Molecular mark accelerates the process of cabbage type rape SOYBEAN IN HIGH-YIELD BREEDING, is suitable for large-scale promotion application.
Another object of the present invention is to provide a kind of SNP in the main effect QTL site of cabbage type rape seed density character
Molecular labeling, ingenious in design, detection is simple and efficient, at low cost, not affected by environment, is suitable for large-scale promotion application.
Another object of the present invention is to provide a kind of SNP in the main effect QTL site of cabbage type rape seed density character
The application of molecular labeling can be used for detecting the size of cabbage type rape seed density, can be used for predicting cabbage type rape
The size of seed density can be used for effectively selecting the size of cabbage type rape seed density, can be also used for seed
The molecular mark of the big cabbage type rape of density accelerates the process of cabbage type rape SOYBEAN IN HIGH-YIELD BREEDING, is suitable for extensive
It promotes and applies.
Another object of the present invention is to provide a kind of SNP in the main effect QTL site of cabbage type rape seed density character
The application of molecular labeling, ingenious in design, detection is simple and efficient, at low cost, not affected by environment, is answered suitable for large-scale promotion
With.
To achieve the above objectives, in the first aspect of the present invention, a kind of master of cabbage type rape seed density character is provided
QTL site is imitated, its main feature is that, the main effect QTL site of the cabbage type rape seed density character is located at cabbage type rape
Between the bit base of 27888132nd bit base of A09 chromosomes~the 28240668th.
Preferably, the main effect QTL site of the cabbage type rape seed density character is to cabbage type rape seed density
The contribution rate of character is 30.45%.
Preferably, the main effect QTL site of the cabbage type rape seed density character and the first SNP marker are close
Chain, first SNP marker is located at the 27888132nd bit base, and the 27888132nd bit base is A or G,
The mutation leads to polymorphism.
Preferably, the main effect QTL site of the cabbage type rape seed density character and the second SNP marker are close
Chain, second SNP marker is located at the 28240668th bit base, and the 28240668th bit base is A or T,
The mutation leads to polymorphism.
Preferably, the main effect QTL site of the cabbage type rape seed density character and peak value SNP marker are close
Chain, the peak value SNP marker is located at the 28151624th bit base, and the 28151624th bit base is T or G, this is prominent
Change leads to polymorphism.
In the second aspect of the present invention, provide the SNP in the main effect QTL site of cabbage type rape seed density character points a kind of
Son label, its main feature is that, the SNP marker is located at the 27888132nd bit base of the A09 chromosomes of cabbage type rape, institute
It is A or G to state the 27888132nd bit base, which leads to polymorphism.
In the third aspect of the present invention, the SNP in the main effect QTL site of above-mentioned cabbage type rape seed density character is provided
Molecular labeling detection cabbage type rape seed density size, predict cabbage type rape seed density size, to Wild cabbage type
The size of rape seed density carries out the application in the molecular mark of selection or the big cabbage type rape of seed density.
In the fourth aspect of the present invention, provide the SNP in the main effect QTL site of cabbage type rape seed density character points a kind of
Son label, its main feature is that, the SNP marker is located at the 28240668th bit base of the A09 chromosomes of cabbage type rape, institute
It is A or T to state the 28240668th bit base, which leads to polymorphism.
In the fifth aspect of the present invention, the SNP in the main effect QTL site of above-mentioned cabbage type rape seed density character is provided
Molecular labeling detection cabbage type rape seed density size, predict cabbage type rape seed density size, to Wild cabbage type
The size of rape seed density carries out the application in the molecular mark of selection or the big cabbage type rape of seed density.
In the sixth aspect of the present invention, a kind of peak value in the main effect QTL site of cabbage type rape seed density character is provided
SNP marker, its main feature is that, the peak value SNP marker is located at the 28151624th of the A09 chromosomes of cabbage type rape
Bit base, the 28151624th bit base are T or G, which leads to polymorphism.
In the seventh aspect of the present invention, the peak in the main effect QTL site of above-mentioned cabbage type rape seed density character is provided
Be worth SNP marker detection cabbage type rape seed density size, predict cabbage type rape seed density size, to sweet
The size of blue type rape seed density carries out in the molecular mark of selection or the big cabbage type rape of seed density
Using.
Beneficial effects of the present invention essentially consist in：
1, the main effect QTL site of cabbage type rape seed density character of the invention is located at the A09 dyeing of cabbage type rape
Between the bit base of 27888132nd bit base of body~the 28240668th, to the contribution rate of cabbage type rape seed density character
Height plays key effect to the regulation and control of cabbage type rape seed density, can be used as map based cloning and molecular marker assisted selection, fits
In large-scale promotion application.
2, the SNP marker in the main effect QTL site of cabbage type rape seed density character of the invention, including be located at sweet
The SNP marker of 27888132nd bit base of the A09 chromosomes of blue type rape, the A09 chromosomes positioned at cabbage type rape
The 28240668th bit base SNP marker and A09 chromosomes positioned at cabbage type rape the 28151624th bit base
Peak value SNP marker, the size of cabbage type rape seed density can be detected, can predict that cabbage type rape seed is close
The size of degree can effectively select the size of cabbage type rape seed density, can be also used for big sweet of seed density
The molecular mark of blue type rape accelerates the process of cabbage type rape SOYBEAN IN HIGH-YIELD BREEDING, is suitable for large-scale promotion application.
3, the SNP marker in the main effect QTL site of cabbage type rape seed density character of the invention, including be located at sweet
The SNP marker of 27888132nd bit base of the A09 chromosomes of blue type rape, the A09 chromosomes positioned at cabbage type rape
The 28240668th bit base SNP marker and A09 chromosomes positioned at cabbage type rape the 28151624th bit base
Peak value SNP marker, it is ingenious in design, detection it is simple and efficient, it is at low cost, it is not affected by environment, be suitable for large-scale promotion
Using.
4, the application of the SNP marker in the main effect QTL site of cabbage type rape seed density character of the invention, including
Positioned at the application of the SNP marker of the 27888132nd bit base of the A09 chromosomes of cabbage type rape, positioned at cabbage type rape
A09 chromosomes the 28240668th bit base SNP marker application and A09 chromosomes positioned at cabbage type rape
The application of the peak value SNP marker of 28151624th bit base can be used for detecting the big of cabbage type rape seed density
It is small, can be used for predicting the size of cabbage type rape seed density, can be used for the size of cabbage type rape seed density into
Row effectively selection, can be also used for the molecular mark of the big cabbage type rape of seed density, accelerates cabbage type rape
The process of SOYBEAN IN HIGH-YIELD BREEDING is suitable for large-scale promotion application.
5, the application of the SNP marker in the main effect QTL site of cabbage type rape seed density character of the invention, including
Positioned at the application of the SNP marker of the 27888132nd bit base of the A09 chromosomes of cabbage type rape, positioned at cabbage type rape
A09 chromosomes the 28240668th bit base SNP marker application and A09 chromosomes positioned at cabbage type rape
The application of the peak value SNP marker of 28151624th bit base, ingenious in design, detection is simple and efficient, at low cost, not by ring
Border influences, and is suitable for large-scale promotion application.
These and other objects, feature and the advantage of the present invention, is obtained by following detailed descriptions, drawings and claims
To fully demonstrate, and can be achieved by means, product and the combination thereof specially pointed out in appended claims.
Description of the drawings
Fig. 1 is the schematic diagram of the distribution results of the cabbage type rape seed density character of the present invention.
Fig. 2 is the peak value SNP marker in the main effect QTL site using cabbage type rape seed density character in the present invention
Carry out the schematic diagram of allelic analysis.
Specific implementation mode
The present inventor passes through in-depth study, discloses a kind of main effect QTL position of cabbage type rape seed density character for the first time
Point and its SNP marker, effectively can efficiently improve cabbage type rape yield using it.
The main effect QTL site of the cabbage type rape seed density character of the present invention is located at the A09 chromosomes of cabbage type rape
The 27888132nd bit base~28240668th between bit base.
Preferably, the main effect QTL site of the cabbage type rape seed density character is to cabbage type rape seed density
The contribution rate of character is 30.45%.
Preferably, the main effect QTL site of the cabbage type rape seed density character and the first SNP marker are close
Chain, first SNP marker is located at the 27888132nd bit base, and the 27888132nd bit base is A or G,
The mutation leads to polymorphism.
Preferably, the main effect QTL site of the cabbage type rape seed density character and the second SNP marker are close
Chain, second SNP marker is located at the 28240668th bit base, and the 28240668th bit base is A or T,
The mutation leads to polymorphism.
Preferably, the main effect QTL site of the cabbage type rape seed density character and peak value SNP marker are close
Chain, the peak value SNP marker is located at the 28151624th bit base, and the 28151624th bit base is T or G, this is prominent
Change leads to polymorphism.
A kind of SNP marker in the main effect QTL site of cabbage type rape seed density character is also provided, Wild cabbage type is located at
27888132nd bit base of the A09 chromosomes of rape, the 27888132nd bit base are A or G, which causes polymorphic
Property.The first as above-mentioned SNP marker.
The SNP marker for also providing the main effect QTL site of above-mentioned cabbage type rape seed density character is sweet in detection
The size of blue type rape seed density, the size for predicting cabbage type rape seed density, to the big of cabbage type rape seed density
Application in the small molecular mark for carrying out selection or the big cabbage type rape of seed density.
A kind of SNP marker in the main effect QTL site of cabbage type rape seed density character is also provided, Wild cabbage type is located at
28240668th bit base of the A09 chromosomes of rape, the 28240668th bit base are A or T, which causes polymorphic
Property.The second as above-mentioned SNP marker.
The SNP marker for also providing the main effect QTL site of above-mentioned cabbage type rape seed density character is sweet in detection
The size of blue type rape seed density, the size for predicting cabbage type rape seed density, to the big of cabbage type rape seed density
Application in the small molecular mark for carrying out selection or the big cabbage type rape of seed density.
A kind of peak value SNP marker in the main effect QTL site of cabbage type rape seed density character is also provided, is located at sweet
28151624th bit base of the A09 chromosomes of blue type rape, the 28151624th bit base are T or G, which causes more
State property.
The peak value SNP marker for also providing the main effect QTL site of above-mentioned cabbage type rape seed density character is being examined
Survey the size of cabbage type rape seed density, the size of prediction cabbage type rape seed density, to cabbage type rape seed density
Size carry out selection or the big cabbage type rape of seed density molecular mark in application.
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In the following examples, the experimental methods for specific conditions are not specified, usually according to conventional strip
Part such as J. Pehanorm Brookers etc. is write, Molecular Cloning:A Laboratory guide, the third edition, Science Press, the condition described in 2002, or
According to the normal condition proposed by manufacturer.
The measurement of embodiment 1, the phenotype of cabbage type rape seed density character
1. being associated with the measurement of the seed density phenotype of group
(1) to the agronomic and quality traits of 627 parts of core germplasms materials (come from Guizhou Oilseed Rape Institute's seed bank) into
The species test analysis of row field, selects the cabbage type rape advanced generation line of 300 sources all over the world to constitute natural population, wherein wrapping
Include 98 parts of resources, 110 parts of breeding materials, 92 parts of kinds or parent；By region division, wherein 246 parts belong to domestic, 54 parts belong to
Foreign source.3 years 2 points of phenotypic evaluations are completed in the Guiyang Kaiyang County townshiies Qing He and Changshun County Weiyuan town rape base.
(2) using live streaming final singling, line-spacing 40cm, spacing in the rows 25cm, 4 row of each cell.Test material field surrounding kind plant protection
Shield row.
(3) seed density：After plant normal mature, each strain from main inflorescence upper, middle and lower portion take respectively 3,4,3 it is complete
Whole silique, measures the length (do not include carpopodium and fruit beak) of each silique fruit body, as Pod length, and unit is centimetre essence
Really to 0.1cm, and the seed grain number of each silique is counted, unit is grain, and seed density is exactly the total seed grain of single plant
The total Pod length of number/single plant.The phenotypic number of all environment of 300 parts of materials is averaged, is as a result summarized as follows：
The average value of the seed density phenotypic number of all environment of 1 300 parts of materials of table
The seed density distribution results of association group show that the distribution of seed density trait expression is distributed in continuity, are in normal state
Distribution, it was demonstrated that seed density character belongs to quantitative character and there are major gene locis, as shown in Figure 1.
2. being associated with the acquisition of group's high quality SNP data sets
Blade total DNA, the blade total DNA of every part of material of extraction association group, specific method are extracted using CTAB methods
For：
Young leaflet tablet is set in 10% ethyl alcohol and is rinsed；Then clip 0.1-0.2g blades, which are put into, grinds in alms bowl, fast using liquid nitrogen
Speed is milled to powdered, is fitted into 2mL centrifuge tubes；700 μ L of preheating DNA extracting solutions are added；1h in 65 DEG C of water-baths of mixing postposition,
Every 10-15min mixings 1 time；700 μ L mixed liquor (phenol are added：Chloroform:Isoamyl alcohol=25: 24: 1), gently overturning mixing
10min；At room temperature, 10 000 × g centrifuges 15min；In Aspirate supernatant to new 2mL centrifuge tubes；Isometric mixed liquor is added
(chloroform: isoamyl alcohol=24: 1), overturn mixing, stand 5min, 10000 × g, centrifuge 15min, with rifle Aspirate supernatant to newly
In centrifuge tube；2 times of volume absolute ethyl alcohols are added, stands 1h, 10 000 × g at -20 DEG C after mixing, centrifuges 10min, abandon supernatant
Liquid；The 75% ethyl alcohol washing precipitation for adding 500 μ L precoolings, removes supernatant；After continuous 2 times washing precipitations, dry；It is added
Containing 100 μ L of 2%RNase solution As, 4 DEG C are stood after 1h at 37 DEG C overnight；With isometric mixed liquor (chloroform: isoamyl alcohol=24:
1) DNA solution is extracted again, overturns mixing, stands 10min, 10 000 × g, centrifuges 15 or 20min, removes RNase A, is drawn
Supernatant (about 60 μ L), centrifuges, 1min again；Utilize agarose gel electrophoresis (0.8%) and UV spectrophotometer measuring DNA
Concentration, quality and integrality；Determine 260/280 ratio of absorbance of all DNA samples between 1.8-2.0.Then by DNA samples
Product dry ice is transported to sequencing company (Hua Da Gene Tech. Company Limited), and each material sequencing depth is about 9 ×.
After obtaining high quality DNA according to above description, sequencing company (Hua Da Gene Tech. Company Limited) carries out 9 × covering
The data returned after the sequencing of depth first use FastQC softwares to carry out sequencing quality assessment, are then carried out to sequencing sequence
Adapter and low quality reads filterings.Obtain each material both-end sequencing clean data, then use bwa softwares into
Row mapping and GATK software are detected into row variation, after obtaining the total SNP data sets of association group, according to minimum equipotential base
Because of frequency >=0.05, miss rate≤0.1 and heterozygosis rate≤0.15 carry out SNP data set mass filters, finally obtain high quality
Group's SNP data sets are used for subsequent analysis.
3. whole-genome association
Using the VCF files progress format conversion for the high quality SNP data sets that plink softwares generate previous step, then
Obtained seed density phenotype and SNP data sets are subjected to full genome association analysis using EMMAX softwares, obtain seed density
The P values in each site of shape, when P values are less than 5 × 10-7SNP be notable SNP, the SNP of P value minimums is peak value SNP, by peak
Material is grouped by different allelic gene types of the value SNP in group, carries out variance analysis, between-group variance and population variance
The percentage of ratio, the as contribution rate of peak value SNP.
By analysis, the section in the main effect QTL site of cabbage type rape seed density character is limited to cabbage type rape
A09 chromosomes the 27888132nd bit base~28240668th between bit base, corresponding SNP is chrA09_
27888132 (A/G), chrA09_28240668 (A/T), peak value SNP are：ChrA09_28151624 (G/T), the QTL is to wild cabbage
The contribution rate of type rape seed density character be 30.45% (material is grouped according to the different allelic gene types of peak value SNP,
The percentage of progress one-way analysis of variance, between-group variance divided by population variance is contribution rate).
The peak value SNP of seed density character is：ChrA09_28151624 (G/T), corresponding seed density phenotype are grouped into：
When the SNP of the positions chrA09_28151624 is GG, the average seed density of material is 3.33/cm；When GT, material is put down
Equal seed density is 3.00/cm；When TT, the average seed density of material is 2.72/cm, as shown in Figure 2.
One of seed density character boundary SNP is：ChrA09_27888132 (A/G), corresponding seed density phenotype
It is grouped into：When the SNP of the positions chrA09_27888132 is GG, the average seed density of material is 2.79/cm；When AG, material
The average seed density of material is 2.94/cm；When AA, the average seed density of material is 3.24/cm, the tribute of boundary SNP
It is 11.85% to offer rate.
Another boundary SNP of seed density character is：ChrA09_28240668 (A/T), corresponding seed density phenotype point
Group is：When the SNP of the positions chrA09_28240668 is TT, the average seed density of material is 2.85/cm；When AT, material
Average seed density be 2.85/cm；When AA, the average seed density of material is 3.29/cm, the contribution of boundary SNP
Rate is 17.87%.
The whole genome sequence of cabbage type rape has been announced, and sees http://www.genoscope.cns.fr/
brassicanapus/.Include front and back each 400bp sequences (the total 801bp) such as SEQ ID NO of chrA09_27888132 (A/G):
Shown in 1, include front and back each 400bp sequences (the total 801bp) such as SEQ ID NO of chrA09_28240668 (A/T):Shown in 2, packet
Front and back each 400bp sequences (total 801bp) such as SEQ ID NO containing chrA09_28151624 (G/T):Shown in 3.Art technology
The specific primer that conventional method detects SNP site according to above-mentioned sequence design may be used in personnel, to detect SNP site
Genotype can predict the size of cabbage type rape seed density it is possible thereby to detect the size of cabbage type rape seed density,
The size of cabbage type rape seed density can effectively be selected, can be also used for the big cabbage type rape of seed density
Molecular mark accelerates the process of cabbage type rape SOYBEAN IN HIGH-YIELD BREEDING.
Therefore, the present invention resurveys sequence by the phenotypic analysis and full-length genome of seed density character, then carries out full genome
Group association analysis, detects the main effect of a cabbage type rape seed density character on cabbage type rape A09 chromosomes
QTL site, the contribution rate to cabbage type rape seed density are 30.45%.The main effect of the cabbage type rape seed density character
QTL site is located between the bit base of the 27888132nd bit base of the A09 chromosomes of cabbage type rape~the 28240668th, boundary
Notable SNP is chrA09_27888132 (A/G), and chrA09_28240668 (A/T), peak value SNP are chrA09_28151624
(G/T), the SNP marker according to this with main effect QTL site close linkage can be used for detecting cabbage type rape seed density
Size, can be used for predict cabbage type rape seed density size, can be used for the big of cabbage type rape seed density
It is small effectively to be selected, the molecular mark of the big cabbage type rape of seed density is can be also used for, Wild cabbage type is accelerated
The process of yield of Brassica napus L breeding.
The SNP marker announced through the invention carries out molecular marker assisted selection, and identification method is simple, efficiency of selection
Cabbage type rape seed density can be predicted in height.Selection target is clear, not protected from environmental.It can be given birth in cabbage type rape early
Phase identifies the big cabbage type rape single plant of seed density, eliminates other single plants.
To sum up, the main effect QTL site of cabbage type rape seed density character of the invention is to cabbage type rape seed density
The contribution rate of character is high, plays key effect to the regulation and control of cabbage type rape seed density, can be used as map based cloning and molecule mark
Remember assisted Selection, is suitable for large-scale promotion application.
It can be seen that the purpose of the present invention is achieved completely and effectively.The function and structural principle of the present invention
It is shown and is illustrated in embodiment, under without departing substantially from the principle, embodiment can make arbitrary modification.So this hair
Bright includes all variant embodiments based on claim spirit and right.
Claims (11)
1. a kind of main effect QTL site of cabbage type rape seed density character, which is characterized in that the cabbage type rape seed
The main effect QTL site of density character is located at the 27888132nd bit base~28240668th of the A09 chromosomes of cabbage type rape
Between bit base.
2. the main effect QTL site of cabbage type rape seed density character as described in claim 1, which is characterized in that described
The main effect QTL site of cabbage type rape seed density character is 30.45% to the contribution rate of cabbage type rape seed density character.
3. the main effect QTL site of cabbage type rape seed density character as described in claim 1, which is characterized in that described
The main effect QTL site of cabbage type rape seed density character and the first SNP marker close linkage, the first SNP molecules
Label is located at the 27888132nd bit base, and the 27888132nd bit base is A or G, which leads to polymorphism.
4. the main effect QTL site of cabbage type rape seed density character as described in claim 1, which is characterized in that described
The main effect QTL site of cabbage type rape seed density character and the second SNP marker close linkage, the 2nd SNP molecules
Label is located at the 28240668th bit base, and the 28240668th bit base is A or T, which leads to polymorphism.
5. the main effect QTL site of cabbage type rape seed density character as described in claim 1, which is characterized in that described
The main effect QTL site of cabbage type rape seed density character and peak value SNP marker close linkage, the peak value SNP molecules
Label is located at the 28151624th bit base, and the 28151624th bit base is T or G, which leads to polymorphism.
6. a kind of SNP marker in the main effect QTL site of cabbage type rape seed density character, which is characterized in that the SNP
Molecular labeling is located at the 27888132nd bit base of the A09 chromosomes of cabbage type rape, the 27888132nd bit base be A or
G, the mutation lead to polymorphism.
7. the SNP marker in the main effect QTL site of cabbage type rape seed density character according to claim 6 is being examined
Survey the size of cabbage type rape seed density, the size of prediction cabbage type rape seed density, to cabbage type rape seed density
Size carry out selection or the big cabbage type rape of seed density molecular mark in application.
8. a kind of SNP marker in the main effect QTL site of cabbage type rape seed density character, which is characterized in that the SNP
Molecular labeling is located at the 28240668th bit base of the A09 chromosomes of cabbage type rape, the 28240668th bit base be A or
T, the mutation lead to polymorphism.
9. the SNP marker in the main effect QTL site of cabbage type rape seed density character according to claim 8 is being examined
Survey the size of cabbage type rape seed density, the size of prediction cabbage type rape seed density, to cabbage type rape seed density
Size carry out selection or the big cabbage type rape of seed density molecular mark in application.
10. a kind of peak value SNP marker in the main effect QTL site of cabbage type rape seed density character, which is characterized in that institute
The 28151624th bit base that peak value SNP marker is located at the A09 chromosomes of cabbage type rape is stated, it is 28151624th described
Base is T or G, which leads to polymorphism.
11. the peak value SNP molecule marks in the main effect QTL site of cabbage type rape seed density character according to claim 10
Remember the size in detection cabbage type rape seed density, the size of prediction cabbage type rape seed density, to cabbage type rape seed
The size of granule density carries out the application in the molecular mark of selection or the big cabbage type rape of seed density.
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| CN109762926A (en) * | 2019-03-20 | 2019-05-17 | 中国农业科学院油料作物研究所 | A kind of and the associated molecular labeling primer of siliqua of oilseed rape number and application |
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| CN111118202A (en) * | 2020-02-25 | 2020-05-08 | 贵州省油菜研究所 | C03 chromosome major QTL site with oleic acid content character of brassica napus seeds, SNP molecular marker and application |
| CN111118204A (en) * | 2020-02-25 | 2020-05-08 | 贵州省油菜研究所 | A08 chromosome major QTL site with oleic acid content character of brassica napus seeds, SNP molecular marker and application |
| CN111118202B (en) * | 2020-02-25 | 2023-04-07 | 贵州省油菜研究所 | C03 chromosome major QTL (quantitative trait locus) site with oleic acid content character of brassica napus seeds, SNP (single nucleotide polymorphism) molecular marker and application |
| CN111118204B (en) * | 2020-02-25 | 2023-04-14 | 贵州省油菜研究所 | A08 chromosome major QTL (quantitative trait locus) site with oleic acid content character of brassica napus seeds, SNP molecular marker and application |
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