CN108728574B - Major QTL (quantitative trait locus) site for cabbage type rape grain density character, SNP (Single nucleotide polymorphism) molecular marker and application - Google Patents

Major QTL (quantitative trait locus) site for cabbage type rape grain density character, SNP (Single nucleotide polymorphism) molecular marker and application Download PDF

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CN108728574B
CN108728574B CN201810641612.7A CN201810641612A CN108728574B CN 108728574 B CN108728574 B CN 108728574B CN 201810641612 A CN201810641612 A CN 201810641612A CN 108728574 B CN108728574 B CN 108728574B
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向阳
杜才富
唐敏强
刘胜毅
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GUIZHOU RAPE INSTITUTE
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Abstract

The invention provides a main effect QTL locus of a cabbage type rape seed density character, which is positioned between the 27888132 th base and the 28240668 th base of an A09 chromosome of the cabbage type rape. Preferably, the contribution rate to the cabbage type rape kernel density character is 30.45%. Closely linked to the first SNP molecular marker, which is located at base 27888132 and is either A or G, the mutation results in a polymorphism. Closely linked to a second SNP molecular marker, which is located at base 28240668 and is either A or T, the mutation resulting in a polymorphism. Closely linked to the peak SNP marker, which is located at base 28151624 and is either T or G, the mutation results in a polymorphism. Also provides related SNP molecular markers and application. The main effect QTL site of the cabbage type rape seed density character has high contribution rate to the cabbage type rape seed density character, plays a key role in regulating and controlling the cabbage type rape seed density, can be used for map location cloning and molecular marker assisted selection, and is suitable for large-scale popularization and application.

Description

Major QTL (quantitative trait locus) site for cabbage type rape grain density character, SNP (Single nucleotide polymorphism) molecular marker and application
Technical Field
The invention relates to the technical field of molecular biology and rape breeding, in particular to the technical field of the density character of brassica napus seeds, and specifically relates to a major QTL (quantitative trait locus) of the density character of the brassica napus seeds, an SNP (single nucleotide polymorphism) molecular marker and application thereof.
Background
Rape belonging to the genus Brassica (Brassica) of the family Brassicaceae (Cruciferae) is an important superior oil crop. Depending on the phytological and biological characteristics, oilseed rape can be divided into three main types: brassica napus (l.; 2n ═ 20, aa), Brassica juncea (coss.; 2n ═ 36, aabb), and Brassica napus (l.; 2n ═ 38, aacc). Cabbage type rape is one of the most important oil crops in the world, particularly in Asia, Europe and North America, and is also the first oil crop in China, and rapeseed oil accounts for more than 55% of edible vegetable oil produced in China. In recent years, with the continuous rise of labor price and production cost, the production and planting area of rape in China is reduced and the rape seed is in short supply, and correspondingly, the import quantity of the rape seed is continuously increased (http:// m. In the face of the severe situation of rape supply shortage in China, rape breeders in China put forward the fourth time of rape industry leap development targets marked by ' three high ' (high yield, high oil content and high efficiency) ' (Wanghongzhong (2010) history review and prospect of rape industry development in China. Chinese oil crop academic newspaper 32(2): 300-302). The cultivation of the new high-yield rape variety is one of the main tasks of rape breeding in the future, and important guarantee is certainly provided for the development of the rape industry and the oil safety in China.
The rape pod is used as an important organ of 'library' and 'source', and is directly related to the accumulation of photosynthetic products, so that the yield of the rape is influenced. The quantity of the single plant siliques and the quantity of the seeds per fruit are the constituent elements of the rape yield, and the silique density and the kernel survival density in the siliques respectively influence the quantity of the single plant siliques and the quantity of the seeds per fruit and have direct or indirect influence on the rape yield. Therefore, increasing the density of the siliques and the kernel survival density in the siliques becomes a possible way to improve the yield of the rapes, and has important significance for the research of the siliques and the related characters in the rape genetic breeding.
Therefore, a main effect QTL site for the cabbage type rape seed density character needs to be provided, has high contribution rate to the cabbage type rape seed density character, plays a key role in regulating and controlling the cabbage type rape seed density, and can be used for site cloning and molecular marker assisted selection.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide the main effect QTL locus for the cabbage type rape seed density character, which has high contribution rate to the cabbage type rape seed density character, plays a key role in the regulation and control of the cabbage type rape seed density, can be used for site cloning and molecular marker assisted selection, and is suitable for large-scale popularization and application.
The invention also aims to provide the SNP molecular marker of the major QTL site of the cabbage type rape seed density character, which can detect the density of the cabbage type rape seed, can predict the density of the cabbage type rape seed, can effectively select the density of the cabbage type rape seed, can be used for molecular marker-assisted breeding of the cabbage type rape with high seed density, accelerates the process of high-yield breeding of the cabbage type rape, and is suitable for large-scale popularization and application.
The invention also aims to provide the SNP molecular marker of the major QTL site of the cabbage type rape seed density character, which has the advantages of ingenious design, simple and quick detection, low cost, no environmental influence and suitability for large-scale popularization and application.
The invention also aims to provide application of the SNP molecular marker of the major QTL site of the cabbage type rape seed density character, which can be used for detecting the density of the cabbage type rape seed, predicting the density of the cabbage type rape seed, effectively selecting the density of the cabbage type rape seed, and accelerating the process of high-yield breeding of the cabbage type rape by using the molecular marker of the cabbage type rape with high seed density, and is suitable for large-scale popularization and application.
The invention also aims to provide application of the SNP molecular marker of the main effect QTL locus of the cabbage type rape seed density character, which has the advantages of ingenious design, simple and quick detection, low cost, no environmental influence and suitability for large-scale popularization and application.
In order to achieve the above object, in a first aspect of the present invention, a major QTL locus for the brassica napus kernel density trait is provided, wherein the major QTL locus for the brassica napus kernel density trait is located between the 27888132 st base and the 28240668 th base of the chromosome a09 of brassica napus.
Preferably, the contribution rate of the main effect QTL site of the cabbage type rape seed density character to the cabbage type rape seed density character is 30.45%.
Preferably, the major QTL site of the cabbage type rape seed density trait is closely linked with a first SNP molecular marker, the first SNP molecular marker is located at the 27888132 th base, the 27888132 th base is A or G, and the mutation causes polymorphism.
Preferably, the major QTL site of the cabbage type rape seed density trait is closely linked with a second SNP molecular marker, the second SNP molecular marker is located at the 28240668 th base, the 28240668 th base is A or T, and the mutation causes polymorphism.
Preferably, the major QTL site of the cabbage type rape seed density trait is closely linked with a peak SNP molecular marker, the peak SNP molecular marker is located at 28151624 th base, the 28151624 th base is T or G, and the mutation causes polymorphism.
In a second aspect of the invention, the SNP molecular marker of the major QTL site of the cabbage type rape seed density trait is provided, and is characterized in that the SNP molecular marker is located at the 27888132 th base of the chromosome A09 of the cabbage type rape, the 27888132 th base is A or G, and the mutation causes polymorphism.
In a third aspect of the present invention, an application of the SNP molecular marker of the major QTL locus for the brassica napus kernel density trait described above in detecting the size of the brassica napus kernel density, predicting the size of the brassica napus kernel density, selecting the size of the brassica napus kernel density, or molecular marker-assisted breeding of brassica napus with a large kernel density is provided.
In the fourth aspect of the invention, the SNP molecular marker of the major QTL site of the cabbage type rape seed density trait is provided, and is characterized in that the SNP molecular marker is located at the 28240668 th base of the A09 chromosome of the cabbage type rape, the 28240668 th base is A or T, and the mutation causes polymorphism.
In the fifth aspect of the invention, the application of the SNP molecular marker of the major QTL site for the cabbage rape seed density trait described above in detecting the density of the cabbage rape seed, predicting the density of the cabbage rape seed, selecting the density of the cabbage rape seed or performing molecular marker assisted breeding of the cabbage rape with high seed density is provided.
In a sixth aspect of the invention, the peak SNP molecular marker of the major QTL site of the cabbage type rape seed density trait is provided, and is characterized in that the peak SNP molecular marker is located at the 28151624 th base of the A09 chromosome of the cabbage type rape, the 28151624 th base is T or G, and the mutation causes polymorphism.
In the seventh aspect of the present invention, an application of the peak SNP molecular marker of the major QTL locus for the brassica napus kernel density trait in detecting the density of brassica napus kernels, predicting the density of brassica napus kernels, selecting the density of brassica napus kernels, or in molecular marker-assisted breeding of brassica napus with high kernel density is provided.
The invention has the following beneficial effects:
1. the main effect QTL locus of the cabbage type rape seed density character is positioned between the 27888132 th base and the 28240668 th base of the chromosome A09 of the cabbage type rape, has high contribution rate to the cabbage type rape seed density character, plays a key role in the regulation and control of the cabbage type rape seed density, can be used for map-based cloning and molecular marker-assisted selection, and is suitable for large-scale popularization and application.
2. The SNP molecular marker of the major QTL site of the cabbage type rape seed density character comprises an SNP molecular marker of a 27888132 th base of an A09 chromosome of the cabbage type rape, an SNP molecular marker of a 28240668 th base of an A09 chromosome of the cabbage type rape and a peak SNP molecular marker of a 28151624 th base of an A09 chromosome of the cabbage type rape, can detect the density of the cabbage type rape seed, can predict the density of the cabbage type rape seed, can effectively select the density of the cabbage type rape seed, can be used for molecular marker-assisted breeding of the cabbage type rape with high seed density, accelerates the process of high-yield breeding of the cabbage type rape, and is suitable for large-scale popularization and application.
3. The SNP molecular marker of the major QTL site of the cabbage type rape grain density trait comprises an SNP molecular marker of a 27888132 th base of an A09 chromosome of the cabbage type rape, an SNP molecular marker of a 28240668 th base of an A09 chromosome of the cabbage type rape and a peak SNP molecular marker of a 28151624 th base of an A09 chromosome of the cabbage type rape.
4. The application of the SNP molecular marker of the major QTL site of the cabbage type rape seed density character comprises the application of the SNP molecular marker of the 27888132 th base of the A09 chromosome of the cabbage type rape, the application of the SNP molecular marker of the 28240668 th base of the A09 chromosome of the cabbage type rape and the application of the peak SNP molecular marker of the 28151624 th base of the A09 chromosome of the cabbage type rape.
5. The application of the SNP molecular marker of the major QTL site of the cabbage type rape grain density trait comprises the application of the SNP molecular marker of the 27888132 th base of the A09 chromosome of the cabbage type rape, the application of the SNP molecular marker of the 28240668 th base of the A09 chromosome of the cabbage type rape and the application of the peak SNP molecular marker of the 28151624 th base of the A09 chromosome of the cabbage type rape.
These and other objects, features and advantages of the present invention will become more fully apparent from the following detailed description, the accompanying drawings and the claims, and may be realized by means of the instrumentalities, products and combinations particularly pointed out in the appended claims.
Drawings
FIG. 1 is a schematic diagram of the distribution result of the cabbage type rape kernel density trait of the present invention.
FIG. 2 is a schematic diagram of allelic analysis by using peak SNP molecular markers of major QTL sites of the cabbage type rape grain density trait.
Detailed Description
Through intensive research, the inventor firstly discloses a main effect QTL site of the cabbage type rape seed density character and an SNP molecular marker thereof, and can effectively and efficiently improve the yield of the cabbage type rape by utilizing the main effect QTL site.
The main effect QTL locus of the cabbage type rape seed density character is positioned between the 27888132 th base and the 28240668 th base of the chromosome A09 of the cabbage type rape.
Preferably, the contribution rate of the main effect QTL site of the cabbage type rape seed density character to the cabbage type rape seed density character is 30.45%.
Preferably, the major QTL site of the cabbage type rape seed density trait is closely linked with a first SNP molecular marker, the first SNP molecular marker is located at the 27888132 th base, the 27888132 th base is A or G, and the mutation causes polymorphism.
Preferably, the major QTL site of the cabbage type rape seed density trait is closely linked with a second SNP molecular marker, the second SNP molecular marker is located at the 28240668 th base, the 28240668 th base is A or T, and the mutation causes polymorphism.
Preferably, the major QTL site of the cabbage type rape seed density trait is closely linked with a peak SNP molecular marker, the peak SNP molecular marker is located at 28151624 th base, the 28151624 th base is T or G, and the mutation causes polymorphism.
Also provides an SNP molecular marker of a main effect QTL site of the cabbage type rape grain density trait, which is located at the 27888132 th base of the chromosome A09 of the cabbage type rape, wherein the 27888132 th base is A or G, and the mutation causes polymorphism. Namely the first SNP molecular marker.
Also provides the application of the SNP molecular marker of the main effect QTL site of the cabbage type rape seed density character in detecting the density of the cabbage type rape seed, predicting the density of the cabbage type rape seed, selecting the density of the cabbage type rape seed or carrying out molecular marker assisted breeding on the cabbage type rape with high seed density.
Also provides an SNP molecular marker of a main effect QTL site of the cabbage type rape grain density trait, which is located at the 28240668 th base of the chromosome A09 of the cabbage type rape, wherein the 28240668 th base is A or T, and the mutation causes polymorphism. Namely the second SNP molecular marker.
Also provides the application of the SNP molecular marker of the main effect QTL site of the cabbage type rape seed density character in detecting the density of the cabbage type rape seed, predicting the density of the cabbage type rape seed, selecting the density of the cabbage type rape seed or carrying out molecular marker assisted breeding on the cabbage type rape with high seed density.
Also provides a peak SNP molecular marker of a main effect QTL site of the cabbage type rape grain density trait, which is located at the 28151624 th base of the A09 chromosome of the cabbage type rape, wherein the 28151624 th base is T or G, and the mutation causes polymorphism.
Also provides the application of the peak SNP molecular marker of the main effect QTL site of the cabbage type rape seed density character in detecting the density of the cabbage type rape seed, predicting the density of the cabbage type rape seed, selecting the density of the cabbage type rape seed or carrying out molecular marker assisted breeding of the cabbage type rape with high seed density.
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The experimental procedures, for which specific conditions are not noted in the following examples, are generally performed according to conventional conditions such as those described in J. SammBruk et al, molecular cloning protocols, third edition, scientific Press, 2002, or according to the manufacturer's recommendations.
Example 1 determination of phenotype of cabbage type rape seed Density trait
1. Determination of kernel density phenotype of related populations
(1) Carrying out field seed test analysis on the agronomic and quality traits of 627 parts of core germplasm materials (from a seed bank of the oil-vegetable research institute in Guizhou province), and selecting 300 high-generation brassica napus strains from all over the world to form a natural population, wherein the natural population comprises 98 parts of resources, 110 parts of breeding materials and 92 parts of varieties or parents; the method is divided into regions, wherein 246 parts belong to domestic and 54 parts belong to foreign sources. 3-year 2-point phenotype identification is completed on rape bases in Weiyuan village and Changxing town of Kaiyang county in Guiyang city.
(2) Direct seeding and final singling are adopted, the row spacing is 40cm, the planting spacing is 25cm, and 4 rows are arranged in each cell. And (4) planting protective rows around the test material field.
(3) Seed density: after the plants are normally mature, 3, 4 and 3 complete siliques are respectively taken from the upper part, the middle part and the lower part of a main inflorescence of each plant, the length of the body of each silique (excluding a stem and a beak) is measured to be the length of the silique, the unit is centimeter, the accuracy is 0.1cm, the number of grains of each silique is counted, the unit is grain, and the density of the grains is the total number of grains per plant/total length of the silique per plant. The tabular values for all environments were averaged for 300 parts of material and the results are summarized as follows:
table 1300 materials average of grain density profile values for all environments
Figure BDA0001702533320000071
Figure BDA0001702533320000081
The kernel density distribution result of the associated population shows that the kernel density character performance distribution is in continuous distribution and normal distribution, and the kernel density character is proved to belong to quantitative characters and have major gene loci, as shown in figure 1.
2. Acquisition of related population high quality SNP data set
The method comprises the following steps of (1) extracting total DNA of leaves by adopting a CTAB method, and extracting the total DNA of the leaves of each material of a related population, wherein the specific method comprises the following steps:
rinsing the young and tender leaves in 10% ethanol; then shearing 0.1-0.2g of blades, putting the blades into a bowl mill, quickly milling the blades into powder by using liquid nitrogen, and putting the powder into a 2mL centrifuge tube; adding 700 mu L of preheated DNA extracting solution; mixing, placing in 65 deg.C water bath for 1h, and mixing for 1 time every 10-15 min; adding 700 μ L of mixed solution (phenol: chloroform: isoamyl alcohol 25: 24: 1), and mixing by gentle inversion for 10 min; centrifuging at room temperature at 10000 Xg for 15 min; sucking the supernatant into a new 2mL centrifuge tube; adding equal volume of mixed solution (chloroform: isoamyl alcohol is 24: 1), mixing, standing for 5min, centrifuging for 15min at 10000 Xg, and sucking supernatant into a new centrifuge tube with a gun; adding 2 times volume of anhydrous ethanol, mixing, standing at-20 deg.C for 1 hr at 10000 Xg, centrifuging for 10min, and removing supernatant; adding 500 μ L of precooled 75% ethanol, washing the precipitate, and removing the supernatant; washing and precipitating for 2 times continuously, and then airing; adding 100 μ L RNase A solution containing 2% RNase A, standing at 37 deg.C for 1h, and standing at 4 deg.C overnight; re-extracting DNA solution with equal volume of mixed solution (chloroform: isoamyl alcohol: 24: 1), reversing, mixing, standing for 10min, 10000 Xg, centrifuging for 15 or 20min, removing RNase A, sucking supernatant (about 60 μ L), and centrifuging again for 1 min; detecting the concentration, quality and integrity of the DNA by agarose gel electrophoresis (0.8%) and an ultraviolet spectrophotometer; the ratio of the absorbance 260/280 was determined to be between 1.8 and 2.0 for all DNA samples. The DNA samples were then transported on dry ice to sequencing Inc. (Huada science and technology, Inc.), each material having a sequencing depth of about 9X.
After obtaining high quality DNA as described above, the sequencing company (Huada Gene science and technology Co., Ltd.) performed 9 Xcoverage depth sequencing and returned data, and the sequencing quality was evaluated by using FastQC software, and then adapter and low quality reads were performed on the sequencing sequence. Obtaining clear data of double-end sequencing of each material, then using bwa software to carry out mapping and GATK software to carry out mutation detection, and after obtaining a total SNP data set of an associated group, carrying out SNP data set quality filtering according to the minimum allele frequency of more than or equal to 0.05, the deletion rate of less than or equal to 0.1 and the heterozygosity rate of less than or equal to 0.15, and finally obtaining a high-quality group SNP data set for subsequent analysis.
3. Whole genome association analysis
Format conversion is carried out on the VCF file of the high-quality SNP data set generated in the last step by using plink software, and then the obtained grain density phenotype and SNP data set are subjected to whole-gene association analysis by using EMMAX software to obtain grain densityP value of each site of the character, when the P value is less than 5X 10-7The SNP is the obvious SNP, the SNP with the minimum P value is the peak SNP, the materials are grouped by different allele types of the peak SNP in a group, variance analysis is carried out, and the percentage of the ratio of the variance between the groups to the total variance is the contribution rate of the peak SNP.
Through analysis, the interval of the major QTL locus of the cabbage type rape grain density trait is limited between the 27888132 th base and the 28240668 th base of the A09 chromosome of the cabbage type rape, the corresponding SNPs are chrA 09-27888132 (A/G), chrA 09-28240668 (A/T), and the peak SNP is: and the contribution rate of the QTL to the cabbage rape grain density character is 30.45% (the materials are grouped according to different allele types of peak SNP, single-factor analysis of variance is carried out, and the percentage of the group variance divided by the total variance is the contribution rate).
The peak SNP of the kernel density character is as follows: chrA09_28151624(G/T), corresponding grain density phenotype grouping: when the SNP at the position chrA 09-28151624 is GG, the average grain density of the material is 3.33 grains/cm; GT, the average grain density of the material is 3.00 grains/cm; at TT, the average grain density of the material was 2.72 grains/cm, as shown in FIG. 2.
One of the boundary SNPs for grain density traits is: chrA09_27888132(A/G), corresponding grain density phenotype grouping: when the SNP at the position chrA 09-27888132 is GG, the average grain density of the material is 2.79 grains/cm; when AG is used, the average grain density of the material is 2.94 grains/cm; at AA, the average grain density of the material is 3.24 grains/cm, and the contribution rate of the boundary SNP is 11.85%.
Another boundary SNP for kernel density trait is: chrA09_28240668(A/T), corresponding grain density phenotype grouping: when SNP at position chrA 09-28240668 is TT, the average grain density of the material is 2.85 grains/cm; AT AT, the average grain density of the material is 2.85 grains/cm; at AA, the average grain density of the material is 3.29 grains/cm, and the contribution rate of the boundary SNP is 17.87%.
The whole genome sequence of Brassica napus has been published, see http:// www.genoscope.cns.fr/brassicianapus/. The sequences (801 bp in total) of 400bp in front and back of the sequence containing chrA 09-27888132 (A/G) are shown as SEQ ID NO:1, the sequences (801 bp in total) of 400bp in front and back of the sequence containing chrA 09-28240668 (A/T) are shown as SEQ ID NO:2, and the sequences (801 bp in total) of 400bp in front and back of the sequence containing chrA 09-28151624 (G/T) are shown as SEQ ID NO: 3. The technicians in the field can adopt a conventional method to design a specific primer for detecting the SNP locus according to the sequence so as to detect the genotype of the SNP locus, so that the grain density of the cabbage type rape can be detected, the grain density of the cabbage type rape can be predicted, the grain density of the cabbage type rape can be effectively selected, and the method can be used for molecular marker assisted breeding of the cabbage type rape with high grain density and accelerating the process of high-yield breeding of the cabbage type rape.
Therefore, the main effect QTL site of the cabbage type rape seed density character is detected on the chromosome A09 of the cabbage type rape through phenotype analysis and whole genome re-sequencing of the seed density character, and the contribution rate to the cabbage type rape seed density is 30.45%. The main effect QTL locus of the cabbage type rape seed density character is positioned between the 27888132 th base and the 28240668 th base of an A09 chromosome of the cabbage type rape, the obvious SNP of the boundary is chrA09_27888132(A/G), chrA09_28240668(A/T), the peak SNP is chrA09_28151624(G/T), and according to the SNP molecular marker tightly linked with the main effect QTL locus, the method can be used for detecting the size of the cabbage type rape seed density, predicting the size of the cabbage type rape seed density, effectively selecting the size of the cabbage type rape seed density, and also can be used for molecular marker assisted breeding of the cabbage type rape with large seed density, and accelerating the process of high-yield breeding of the cabbage type rape.
The SNP molecular marker disclosed by the invention is used for carrying out molecular marker-assisted selection, the identification method is simple, the selection efficiency is high, and the density of the cabbage type rape seeds can be predicted. The selection target is clear and is not influenced by the environment. The individual cabbage type rape with large grain density can be identified in the early growth stage of cabbage type rape, and other individual plants are eliminated.
In conclusion, the main effect QTL site of the cabbage type rape seed density character has high contribution rate to the cabbage type rape seed density character, plays a key role in the regulation and control of the cabbage type rape seed density, can be used for site cloning and molecular marker assisted selection, and is suitable for large-scale popularization and application.
It will thus be seen that the objects of the invention have been fully and effectively accomplished. The functional and structural principles of the present invention have been shown and described in the embodiments, and the embodiments may be modified without departing from the principles. Therefore, this invention includes all modifications encompassed within the spirit and scope of the claims.
Figure BDA0001702533320000121
Figure BDA0001702533320000131
Figure IDA0001744136310000011
Figure IDA0001744136310000021

Claims (6)

1. An SNP molecular marker of a main effect QTL locus of a cabbage type rape grain density trait is characterized in that the SNP molecular marker is located at the 27888132 th base of the A09 chromosome of the cabbage type rape, the 27888132 th base is A or G, and the mutation causes polymorphism.
2. The application of the SNP molecular marker of the major QTL site for the cabbage type rape seed density trait of claim 1 in detecting the size of the cabbage type rape seed density, predicting the size of the cabbage type rape seed density, selecting the size of the cabbage type rape seed density or performing molecular marker assisted breeding of the cabbage type rape with high seed density.
3. An SNP molecular marker of a main effect QTL locus of a cabbage type rape grain density trait is characterized in that the SNP molecular marker is located at the 28240668 th base of the A09 chromosome of the cabbage type rape, the 28240668 th base is A or T, and the mutation causes polymorphism.
4. The application of the SNP molecular marker of the major QTL site for the cabbage type rape seed density trait of claim 3 in detecting the size of the cabbage type rape seed density, predicting the size of the cabbage type rape seed density, selecting the size of the cabbage type rape seed density or carrying out molecular marker assisted breeding of the cabbage type rape with large seed density.
5. A peak SNP molecular marker of a main effect QTL site of a cabbage type rape kernel density trait is characterized in that the peak SNP molecular marker is located at the 28151624 th base of the A09 chromosome of the cabbage type rape, the 28151624 th base is T or G, and the mutation causes polymorphism.
6. The application of the peak SNP molecular marker of the major QTL site for the cabbage type rape seed density trait of claim 5 in detecting the density of the cabbage type rape seed, predicting the density of the cabbage type rape seed, selecting the density of the cabbage type rape seed or performing molecular marker assisted breeding of the cabbage type rape with high seed density.
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