CN108728448B - Peanut oil synthesis related gene and application thereof - Google Patents
Peanut oil synthesis related gene and application thereof Download PDFInfo
- Publication number
- CN108728448B CN108728448B CN201810565234.9A CN201810565234A CN108728448B CN 108728448 B CN108728448 B CN 108728448B CN 201810565234 A CN201810565234 A CN 201810565234A CN 108728448 B CN108728448 B CN 108728448B
- Authority
- CN
- China
- Prior art keywords
- seq
- gene
- related gene
- peanut oil
- oil synthesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
Abstract
The invention discloses a peanut oil synthesis related gene and application thereof, belonging to the technical field of biology. The peanut oil synthesis related gene sequence is shown as SEQ ID No.1 or a sequence of one or more basic groups substituted, deleted or added in SEQ ID No.1 and encoding protein with the same function. The peanut oil synthesis related gene is used for constructing a plant expression vector and transforming plants, so that the oil synthesis and stress resistance of the plants can be improved.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a peanut oil synthesis related gene and application thereof.
Background
Peanuts are the main source of edible vegetable oil in China, and the improvement of the oil content becomes one of the most important targets for the quality breeding of the peanuts in China. The average oil content of more than 30 main peanut varieties popularized and planted in China in 20 years is only 51.4%, and the oil content of some large peanut varieties planted in northern main producing areas is less than 50%. And about 50% of peanuts in China are used for oil pressing, and the pure income can be increased by 7% when the oil content is increased by 1%.
At present, a new biological species is generally bred by a conventional hybridization method. The current rapidly developed genetic engineering technology provides a new approach for biological genetic improvement, and genetic transformation by using genes playing an important role in oil synthesis is an important means for obtaining new high-oil germplasm.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a peanut oil synthesis related gene and application thereof.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a peanut oil synthesis related gene has a sequence shown as SEQ ID No.1 or a sequence of one or more basic groups substituted, deleted or added in SEQ ID No.1 and codes the protein with the same function.
On the basis of the scheme, the primer sequence for cloning the gene is as follows:
P1：5′-GGAGCTTCCTGCAACCATCA-3′；
P2：5′-TGCGTATCACCATCAAAACCC-3′。
primers for amplifying any segment of the gene related to oil synthesis also belong to the protection scope of the invention.
Based on the scheme, the gene has no intron, and the coded protein has a conserved sequence proline knot (-PX5SPX 3P-).
A recombinant vector, an expression cassette, a transgenic cell line or a recombinant bacterium containing the peanut oil synthesis related gene.
The protein coded by the peanut oil synthesis related gene.
On the basis of the scheme, the protein amino acid sequence coded by the peanut oil synthesis related gene is shown as SEQ ID No.3 or protein which is subjected to substitution and/or deletion and/or addition of one or more amino acids and has the same function in SEQ ID No. 3.
On the basis of the scheme, the peanut oil synthesis related gene or the protein coded by the gene is applied to improving the biological oil content and the biological stress resistance.
On the basis of the scheme, the method is applied to improving the oil content of the peanuts.
On the basis of the scheme, the stress resistance of the arabidopsis thaliana is improved.
On the basis of the scheme, the stress resistance is salt resistance.
A method for improving oil content and salt tolerance of plants comprises the steps of constructing a plant expression vector by the peanut oil synthesis related gene, introducing the plant expression vector into plant cells, and expressing the plant cells in the plants to obtain plants with improved oil content and high salt tolerance.
The invention has the beneficial effects that:
1. according to the invention, a grease synthesis related gene is cloned from peanuts and named as Oleosin1, sequencing results show that the gene has no intron, the coding region is shown as SEQ ID No.2, the coded protein sequence is shown as SEQ ID No.3, and the coded protein has a conserved sequence proline knot (-PX5SPX 3P-).
2. The plant expression vector of Oleosin1 was constructed and transformed into peanuts, and the results showed that: the oil content of the peanut seeds transferred with the Oleosin1 gene is improved by about 5 percent.
3. A plant expression vector of Oleosin1 is constructed and transformed into Arabidopsis thaliana, and the result shows that: the arabidopsis plant transferred with the Oleosin1 gene has normal morphological development, and the transgenic arabidopsis seedling resists the stress of 100mM NaCl; the expression of the Oleosin1 gene in arabidopsis thaliana can obviously improve the salt tolerance of arabidopsis thaliana.
Drawings
FIG. 1 expression of Oleosin1 following peanut salt stress treatment;
FIG. 2 salt tolerance analysis of transgenic Arabidopsis with Oleosin1 gene (A is control and B is transgenic).
Detailed Description
Terms used in the present invention have generally meanings as commonly understood by one of ordinary skill in the art, unless otherwise specified.
The present invention will be described in further detail with reference to the following data in conjunction with specific examples. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.
Example 1
Peanut variety and source: the florescence salt-tolerant mutant No. 22 generated by the in vitro mutagenesis of the pingyangmycin;
1. clone of peanut stress resistance related gene
Taking the genome DNA of Huayun No. 22 (salt-tolerant mutant generated by Pingyangmycin in vitro mutagenesis) as a template, and carrying out PCR (polymerase chain reaction) on the genome DNA by using a primer pair:
P1：5′-GGAGCTTCCTGCAACCATCA-3′(SEQ ID No.4)；
P2：5′-TGCGTATCACCATCAAAACCC-3′(SEQ ID No.5)；
amplifying stress resistance related genes of the peanuts, wherein the gene sequence is shown as SEQ ID No. 1; sequencing results show that the gene has no intron, and the encoded protein has a conserved sequence proline knot (-PX5SPX3P-), and is named as Oleosin 1.
2. Expression of Oleosin1 following salt stress treatment of peanuts
(1) The "Huayu No. 23" seedlings were treated with 0.7% NaCl, and the leaves of the seedlings were removed at different time intervals and immediately frozen in liquid nitrogen for use. Respectively taking 0.05g of peanut young leaves subjected to stress treatment at different time periods, quickly freezing by using liquid nitrogen, grinding into powder, and extracting RNA by using an RNA extraction kit. The extracted total RNA was treated with DNase I and purified.
(2) The samples were reacted on an ABI 7500FAST type fluorescent quantitative PCR instrument. The 20. mu.L reaction system included: mu.L of 2 XSybrGreen qPCR Master Mix, 20. mu. mol/L forward and reverse primers 0.25. mu.L each, 20ng reverse transcription product. The amplification procedure was: pre-denaturation at 94 ℃ for 2 min; then 40 cycles of reactions are carried out, wherein each cycle comprises denaturation at 94 ℃ for 30s, renaturation at 58 ℃ for 30s and extension at 72 ℃ for 30 s; after the circulation is finished, the temperature is slowly raised to 94 ℃, and a melting curve is prepared. Each reaction was provided with 3 multiple wells.
(3) The primer sequence of the quantitative PCR of the Oleosin1 gene is as follows:
the forward primer sequence is 5'-ATGACTGACCGTACCCAACC-3' (SEQ ID No. 6);
the reverse primer sequence was 5'-CAAGCCCCGCGAACAATA-3' (SEQ ID No. 7).
The internal standard gene Actin primer sequence is as follows:
the forward primer sequence is 5'-GTGGCCGTACAACTGGTATCGT-3' (SEQ ID No. 8);
the reverse primer sequence was 5'-ATGGATGGCTGGAAGAGAACT-3' (SEQ ID No. 9).
The results show that: the change of the expression quantity of the Oleosin1 gene before and after salt stress treatment is obvious, which indicates that the gene expression is influenced by salt stress (figure 1).
Example 2
Coli DH5 α was stored in the laboratory of the genetic research laboratory of Qingdao university of agriculture;
agrobacterium tumefaciens strain GV3101 was purchased from Beijing Tianenzze Gene science and technology, Inc.;
transgenic recipient material was provided by the university of Qingdao agricultural university genetic research laboratory for Arabidopsis thaliana wild-type variety.
1. Construction of Oleosin1 Gene plant expression vector
Taking genome DNA of Huayu No. 22 (salt-tolerant mutant generated by Pingyangmycin in vitro mutagenesis) as a template, and respectively adding KpnI and SacI enzyme cutting sites into upstream and downstream primers during amplification.
Wherein the sequences of the upstream primer and the downstream primer are as follows:
P3：5′-GGTACCGGAGCTTCCTGCAACCATCA-3(KpnI)(SEQ ID No.10)；
P4：5′-GAGCTCTGCGTATCACCATCAAAACCC-3′(SacI)(SEQ ID No.11)。
and recovering a PCR product, connecting the PCR product with a cloning vector pMD18-T (purchased from TaKaRa) under the action of T4DNA ligase, transforming Escherichia coli DH5 α by using the connecting product to obtain a bacterial colony resistant to ampicillin, extracting a recombinant plasmid, carrying out double enzyme digestion by using KpnI and SacI, recovering an enzyme digestion fragment containing an Oleosin1 gene, and cloning the enzyme digestion fragment into a corresponding enzyme digestion site of a plant expression vector Super1300 to obtain the plant expression vector of the gene.
2. The expression vector is used for transforming arabidopsis thaliana,
(1) preparation and activation of agrobacterium recombinant strain and preparation of bacterial liquid: and (3) transforming the recombinant plasmid into an agrobacterium strain GV3101 competent cell by using a liquid nitrogen freeze thawing method, and screening out the recombinant strain containing the recombinant plasmid. Single colony of recombinant strain was picked and inoculated to YEB (Rio)Foipin 50mg/L, kanamycin 50mg/L) liquid medium, culturing at 28 ℃ and 180rpm until OD600 is 0.5-0.8, taking 2mL of bacterial liquid, transferring the bacterial liquid into 50mLYEB (rifampicin 50mg/L, kanamycin 50mg/L) culture medium, and culturing until OD600 is 0.6-0.8. Centrifuging the bacterial liquid at 5000rpm for 15min, and adding 1/2MS B liquid with the same volume5Suspending for later use.
(2) Planting of arabidopsis thaliana: selecting proper arabidopsis seeds, soaking in 1% NaClO for 5min, and washing with sterile water for 4-6 times. Dibbling on the substrate soil.
(3) Agrobacterium-mediated genetic transformation: selecting healthy plants in the initial fruit period, reversely buckling the plants above a container containing the agrobacterium suspension liquid with a pot, immersing the whole inflorescence in the agrobacterium suspension liquid for about 20-30s, and paying attention to the fact that leaves are not contacted with the dip dyeing liquid as far as possible. The pot was removed and placed horizontally in a dark box for about 24 h. Care was taken to maintain a certain humidity. And after 24 hours, placing the treated arabidopsis thaliana plant under the illumination condition of 22-25 ℃ to enable the treated arabidopsis thaliana plant to grow normally. After about 3w mature seeds were harvested.
(4) Screening of transgenic Arabidopsis thaliana
Inoculating the transgenic arabidopsis seeds into 20mL MS (hygromycin 30mg/L) culture medium, culturing at 22 ℃ for about one week, selecting fresh green and strong arabidopsis seedlings, and transplanting the seedlings into matrix soil.
4. PCR detection of transgenic plants
Extracting genome DNA of the transgenic plant, and performing PCR amplification by using the vector sequence and an Oleosin1 gene sequence design primer. The PCR reaction program is: 95 deg.C for 5 min; 95 deg.C, 50s, 56 deg.C, 50s, 72 deg.C, 1min, 30 cycles; 72 deg.C, 10 min.
P5：5′-GCTCCTACAAATGCCATCA-3′(SEQ ID No.12)；
P6：5′-TGCGTATCACCATCAAAACCC-3′(SEQ ID No.13)。
5. Salt tolerance identification of transgenic arabidopsis thaliana
To further analyze the salt tolerance of transgenic plants, transgenic Arabidopsis thaliana homozygous by selfing and non-transgenic Arabidopsis thaliana seeds were inoculated on MS (containing 100mM NaCl) medium. The cells were cultured at 22 ℃ for about 2w, and the results were observed. As a result, it was found that Oleosin1 transgenic Arabidopsis seedlings grew normally, whereas non-transgenic Arabidopsis seedlings yellowed and growth was severely affected (FIG. 2). Therefore, the salt-resistant concentration of the transgenic Arabidopsis seedlings was 100mM or more.
Example 3
1. Construction of Oleosin1 Gene peanut expression vector
Taking the genome DNA of peanut (No. 22 florescence produced salt-tolerant mutant by Pingyangmycin in vitro mutagenesis) as a template, and respectively adding KpnI and SacI enzyme cutting sites into upstream and downstream primers during amplification.
Wherein the sequences of the upstream primer and the downstream primer are as follows:
P3：5′-GGTACCGGAGCTTCCTGCAACCATCA-3(KpnI)(SEQ ID No.10)；
P4：5′-GAGCTCTGCGTATCACCATCAAAACCC-3′(SacI)(SEQ ID No.11)。
and recovering a PCR product, connecting the PCR product with a cloning vector pMD18-T (purchased from TaKaRa) under the action of T4DNA ligase, transforming Escherichia coli DH5 α by using the connecting product to obtain a bacterial colony resistant to ampicillin, extracting a recombinant plasmid, carrying out double enzyme digestion by using KpnI and SacI, recovering an enzyme digestion fragment containing an Oleosin1 gene, and cloning the enzyme digestion fragment into a corresponding enzyme digestion site of a plant expression vector Super1300 to obtain the plant expression vector of the gene.
2. Expression vector transformed peanut
(1) Preparation and activation of agrobacterium recombinant strain and preparation of bacterial liquid: and (3) transforming the recombinant plasmid into agrobacterium strain EHA105 competent cells by using a liquid nitrogen freeze-thawing method, and screening out the recombinant strain containing the recombinant plasmid. A single colony of the recombinant strain is picked up and inoculated into YEB (rifampicin 50mg/L and kanamycin 50mg/L) liquid culture medium, when the colony is cultured at 28 ℃ and 180rpm until OD600 is 0.5-0.8, 2mL of the bacterial liquid is taken and transferred into 50mLYEB (rifampicin 50mg/L and kanamycin 50mg/L) culture medium, and the colony is cultured until OD600 is 0.6-0.8. After centrifugation at 5000rpm for 15min, the suspension was suspended in the same volume of liquid MS B5 for further use.
(2) Separating the peanut cotyledon explants: selecting plump peanut seeds of No. 23, soaking in 70% alcohol for 1min, soaking in 0.1% mercuric chloride for 20min, and washing with sterile water for 4-6 times. The seed coat and hypocotyl were removed and each cotyledon was cut longitudinally into 2 halves.
(3) Agrobacterium-mediated genetic transformation: immersing the cut explant in the prepared agrobacterium liquid, infecting for 10min at 28 ℃ and 90rpm by mild shaking, sucking the residual liquid with sterile filter paper, inoculating to an SIM induction culture medium, and co-culturing for 3d in the dark. Transferring to a SIM induction culture medium added with 250mg/L of cefamycin, embedding the incision end of the explant into the culture medium, culturing for about 2w, inducing cluster buds, and culturing under the following conditions: the light intensity is 1500-.
(4) Transferring the explants forming the cluster buds out to SEM culture medium of 250mg/L cefamycin and 100mg/L kanamycin to screen resistant buds, and culturing 2w under the following culture conditions: the light intensity is 1500-. After 2w of culture, the excised adventitious bud was transferred to SEM medium containing 250mg/L of cefamycin and 150mg/L of kanamycin, and selection of resistant buds and elongation of induced buds were carried out, during which about 4w of culture was subcultured 2-3 times.
3. PCR detection of transgenic plants
Extracting genome DNA of the transgenic plant, and performing PCR amplification by using the vector sequence and an Oleosin1 gene sequence design primer. The PCR reaction program is: 95 deg.C for 5 min; 95 deg.C, 50s, 56 deg.C, 50s, 72 deg.C, 1min, 30 cycles; 72 deg.C, 10 min.
P5：5′-GCTCCTACAAATGCCATCA-3′(SEQ ID No.12)；
P6：5′-TGCGTATCACCATCAAAACCC-3′(SEQ ID No.13)。
4. Oil content determination of transgenic peanut seeds
The transgenic peanut seeds after selfing homozygosis and the non-transgenic peanut seeds (variety flower breeding No. 23) are determined by gas chromatography. The result shows that the oil content of the non-transgenic control (No. 23 florescence) is 53.2 percent, while the oil content of the transgenic peanut seeds is 55.3 to 56.4 percent, which can be averagely improved by about 5 percent.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Sequence listing
<110> Qingdao agricultural university
<120> peanut oil synthesis related gene and application thereof
<130>2018
<160>13
<170>SIPOSequenceListing 1.0
<210>1
<211>590
<212>DNA
<213>Arachis hypogaea Linn.(Arachis hypogaea Linn.)
<400>1
ggagcttcct gcaaccatca atgactgacc gtacccaacc acacactgtc caagtccaca 60
ccacagctgg ccgtttcggc gacaccgctg ccggaactaa ccgctatgcc gacagaggcc 120
cgtcaacatc taaggttatc gccgtcatca ctggactccc tatcggcggc acgttgctat 180
tgttcgcggg gcttgccctt gccggaaccc tgcttgggct ggcggtgacc accccgcttt 240
tcatcctctt cagccctgtc atagttccgg ccatcattgt cgttgggctc tcggtggcgg 300
ggttcttgac gtcaggtgca tgtgggctga cggggctgtc ttcgttctcg tgggtcatga 360
attacatccg gcagacccac ggatcggtgc cggagcagct ggaaatggca aagcaccgca 420
tggctgacgt ggccggttac gttggacaga agacgaagga tgtaggacag aagaccaagg 480
aagttgggca agagatacag accaaggctc aggattcaaa gagaacttga tagaatagtg 540
gtttaagctt aaggatgaaa ggggtctatg ggttttgatg gtgatacgca 590
<210>2
<211>510
<212>DNA
<213>Arachis hypogaea Linn.(Arachis hypogaea Linn.)
<400>2
atgactgacc gtacccaacc acacactgtc caagtccaca ccacagctgg ccgtttcggc 60
gacaccgctg ccggaactaa ccgctatgcc gacagaggcc cgtcaacatc taaggttatc 120
gccgtcatca ctggactccc tatcggcggc acgttgctat tgttcgcggg gcttgccctt 180
gccggaaccc tgcttgggct ggcggtgacc accccgcttt tcatcctctt cagccctgtc 240
atagttccgg ccatcattgt cgttgggctc tcggtggcgg ggttcttgac gtcaggtgca 300
tgtgggctga cggggctgtc ttcgttctcg tgggtcatga attacatccg gcagacccac 360
ggatcggtgc cggagcagct ggaaatggca aagcaccgca tggctgacgt ggccggttac 420
gttggacaga agacgaagga tgtaggacag aagaccaagg aagttgggca agagatacag 480
accaaggctc aggattcaaa gagaacttga 510
<210>3
<211>169
<212>PRT
<213>Arachis hypogaea Linn.(Arachis hypogaea Linn.)
<400>3
Met Thr Asp Arg Thr Gln Pro His Thr Val Gln Val His Thr Thr Ala
1 5 10 15
Gly Arg Phe Gly Asp Thr Ala Ala Gly Thr Asn Arg Tyr Pro Asp Arg
20 25 30
Gly Pro Ser Thr Ser Lys Val Ile Ala Val Ile Thr Gly Leu Pro Ile
35 40 45
Gly Gly Thr Leu Leu Leu Phe Ala Gly Leu Ala Leu Ala Gly Thr Leu
50 55 60
Leu Gly Leu Ala Val Thr Thr Pro Leu Phe Ile Leu Phe Ser Pro Val
65 70 75 80
Ile Val Pro Ala Ile Ile Val Val Gly Leu Ser Val Ala Gly Phe Leu
85 90 95
Thr Ser Gly Ala Cys Gly Leu Thr Gly Leu Ser Ser Phe Ser Trp Val
100 105 110
Met Asn Tyr Ile Arg Gln Thr His Gly Ser Val Pro Glu Gln Leu Glu
115 120 125
Met Ala Lys His Arg Met Ala Asp Val Ala Gly Tyr Val Gly Gln Lys
130 135 140
Thr Lys Asp Val Gly Gln Lys Thr Lys Glu Val Gly Gln Glu Ile Gln
145 150 155 160
Thr Lys Ala Gln AspSer Lys Arg Thr
165
<210>4
<211>20
<212>DNA
<213> Artificial sequence (Arachis hypogaea Linn.)
<400>4
<210>5
<211>21
<212>DNA
<213> Artificial sequence (Arachis hypogaea Linn.)
<400>5
tgcgtatcac catcaaaacc c 21
<210>6
<211>20
<212>DNA
<213> Artificial sequence (Arachis hypogaea Linn.)
<400>6
<210>7
<211>18
<212>DNA
<213> Artificial sequence (Arachis hypogaea Linn.)
<400>7
caagccccgc gaacaata 18
<210>8
<211>22
<212>DNA
<213> Artificial sequence (Arachis hypogaea Linn.)
<400>8
gtggccgtac aactggtatc gt 22
<210>9
<211>21
<212>DNA
<213> Artificial sequence (Arachis hypogaea Linn.)
<400>9
atggatggct ggaagagaac t 21
<210>10
<211>26
<212>DNA
<213> Artificial sequence (Arachis hypogaea Linn.)
<400>10
ggtaccggag cttcctgcaa ccatca 26
<210>11
<211>27
<212>DNA
<213> Artificial sequence (Arachis hypogaea Linn.)
<400>11
gagctctgcg tatcaccatc aaaaccc 27
<210>12
<211>19
<212>DNA
<213> Artificial sequence (Arachis hypogaea Linn.)
<400>12
gctcctacaa atgccatca 19
<210>13
<211>21
<212>DNA
<213> Artificial sequence (Arachis hypogaea Linn.)
<400>13
tgcgtatcac catcaaaacc c 21
Claims (2)
- The application of the peanut oil synthesis related gene shown in SEQ ID No.1 or the protein coded by the gene in improving the biological stress resistance, wherein the organism is arabidopsis thaliana, and the stress resistance is salt tolerance.
- 2. A method for improving the salt tolerance of plants is characterized in that: the gene of the protein of SEQ ID No.3 amplified by SEQ ID No.10 and SEQ ID No.11 is constructed into a plant expression vector and is led into plant cells to be expressed in plants to obtain plants with high salt tolerance, wherein the plants are arabidopsis thaliana.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810565234.9A CN108728448B (en) | 2018-06-04 | 2018-06-04 | Peanut oil synthesis related gene and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810565234.9A CN108728448B (en) | 2018-06-04 | 2018-06-04 | Peanut oil synthesis related gene and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108728448A CN108728448A (en) | 2018-11-02 |
| CN108728448B true CN108728448B (en) | 2020-06-09 |
Family
ID=63932074
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810565234.9A Active CN108728448B (en) | 2018-06-04 | 2018-06-04 | Peanut oil synthesis related gene and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108728448B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106854238A (en) * | 2015-12-08 | 2017-06-16 | 中国农业科学院作物科学研究所 | Plant adversity resistance related protein TabZIP14 and its encoding gene and application |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002323509A1 (en) * | 2001-08-30 | 2003-03-18 | Purdue Research Foundation | Methods to produce transgenic plants resistant to osmotic stress |
| TWI377954B (en) * | 2009-11-25 | 2012-12-01 | Univ China Medical | Oil body carriers, uses in target therapy and/or detection of the same, and fusion proteins comprised therein |
| CN101775381B (en) * | 2010-01-12 | 2012-03-14 | 北京农业生物技术研究中心 | Plant stress tolerance correlative protein kinase, encoding gene and application thereof |
| CN102321665B (en) * | 2011-09-29 | 2013-01-30 | 山东省农业科学院高新技术研究中心 | Method for expressing human humanin protein in peanut seed |
| JP6461604B2 (en) * | 2011-12-27 | 2019-01-30 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション | Process for lipid production |
| CN104404063B (en) * | 2014-12-09 | 2017-07-07 | 福建农林大学 | One cultivate peanut vitamin E synthesis key gene and application |
-
2018
- 2018-06-04 CN CN201810565234.9A patent/CN108728448B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106854238A (en) * | 2015-12-08 | 2017-06-16 | 中国农业科学院作物科学研究所 | Plant adversity resistance related protein TabZIP14 and its encoding gene and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108728448A (en) | 2018-11-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107475210B (en) | Rice bacterial leaf blight resistance related gene OsABA2 and application thereof | |
| AU2019297209B2 (en) | Method of obtaining multi-leaf alfalfa material by means of MsPALM1 artificial site-directed mutant | |
| CN107177610B (en) | Arabidopsis thaliana MPK gene for regulating seed size and method for increasing seed size | |
| CN112779234B (en) | Phyllostachys pubescens PeAPX5 gene and application thereof | |
| CN109355295B (en) | Peanut AhWRKY75 gene and application thereof in improving peanut salt tolerance | |
| CN108707614B (en) | Peanut stress resistance gene and application thereof | |
| CN114908107B (en) | Application of peanut AhAbI5-like gene in improving oil content and/or salt tolerance of plants | |
| CN113234720B (en) | Wheat long-chain non-coding RNAlncR156 and application thereof in regulation and control of wheat response to drought stress | |
| CN113461794B (en) | Kit and method for regulating seed germination and application thereof | |
| CN108728448B (en) | Peanut oil synthesis related gene and application thereof | |
| CN114921583A (en) | QTL for controlling wheat plant height, candidate gene TaDHL-7B thereof and application | |
| CN114958867A (en) | Corn ear grain weight and yield regulation gene KWE2, and encoding protein, functional marker, expression vector and application thereof | |
| CN110407922B (en) | Rice cold-resistant gene qSCT11 and application thereof | |
| CN110468118B (en) | Chimonanthus nitens SUMO E3 ligase gene CpSIZ1 and application thereof | |
| CN114292855A (en) | PagARR9 gene for regulating and controlling growth of xylem of poplar and application thereof | |
| CN102675437B (en) | Method for adjusting size of plant organs and inside asymmetry of floral organs | |
| CN108728447B (en) | Peanut stress resistance related gene and application thereof | |
| CN108948162B (en) | Peanut adversity stress gene AhDOG1L and application thereof | |
| CN114292320B (en) | Peanut SL type oil body protein gene AhOLE2 and application thereof in improving salt tolerance of plants | |
| CN114574499A (en) | Application of OsREP3 gene in controlling drought resistance of rice | |
| CN114231556B (en) | Application of GmECT2 in regulating plant height | |
| CN114989275B (en) | Application of OsERF940 protein in improving rice blast resistance | |
| CN111334514B (en) | Method for improving plant aluminum resistance by applying peanut AhGSNOR gene | |
| CN113913441B (en) | Application of rice nascent polypeptide binding complex alpha subunit NACA gene in osmotic stress resistance of plants | |
| CN110904110B (en) | Application of OsHAP3C gene expression reduction in rice variety with shortened heading period and prolonged growth period |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |