CN108721572B

CN108721572B - A Chinese medicinal composition for treating hepatocarcinoma and liver cirrhosis

Info

Publication number: CN108721572B
Application number: CN201810957513.XA
Authority: CN
Inventors: 黄增琼; 曾凡艳; 赵凯丽
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-08-21
Filing date: 2018-08-21
Publication date: 2021-05-07
Anticipated expiration: 2038-08-21
Also published as: CN108721572A

Abstract

The invention relates to a traditional Chinese medicine composition for treating liver cancer and liver cirrhosis, which comprises 21 traditional Chinese medicinal materials of eupolyphaga sinensis Walker, leech, pangolin scales, endothelium corneum gigeriae galli, human placenta, codonopsis pilosula, astragalus membranaceus, poria cocos, pseudo-ginseng, radix curcumae, bighead atractylodes rhizome, oldenlandia diffusa, hairyvein agrimony, barbed skullcap herb, achyranthes root, pericarpium arecae, combined spicebush root, rhizoma atractylodis, gardenia, beautiful sweetgum fruit and trogopterus dung. The traditional Chinese medicine composition has the effects of promoting blood circulation to remove blood stasis, strengthening spleen and tonifying qi, clearing heat and removing toxicity, eliminating dampness and promoting diuresis, warming and invigorating spleen and kidney and the like, and has a remarkable treatment effect on liver cancer and liver cirrhosis. Animal experiments and clinical application show that the medicine has obvious anti-hepatic fibrosis effect; can obviously improve liver function and enhance the capability of the liver for resisting oxidative damage; can stabilize tumor body and inhibit liver cancer development; can improve the life quality of patients and obviously prolong the life cycle of patients.

Description

A Chinese medicinal composition for treating hepatocarcinoma and liver cirrhosis

Technical Field

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating liver cancer and liver cirrhosis.

Background

Liver cancer is one of common malignant tumors in China, and new cancer cases in China are the first in the world. The human body is an organic whole, and although the curative effect of treating liver cancer in western medicine is obvious, the method mainly aims at local treatment of focus, and most of the method has toxic and side effects. Particularly for patients with advanced liver cancer or liver cancer accompanied with liver cirrhosis, western medicine treatment is often stranded. Emphasizes the characteristics of small toxic and side effects and good curative effect of the traditional Chinese medicine in the aspects of overall regulation, strengthening body resistance to eliminate pathogenic factors, regulating balance and improving immunity, can make up the defects of western medicine, makes a great breakthrough in the treatment of liver cancer and liver cirrhosis and brings good news to patients.

According to traditional Chinese medicine, liver cancer belongs to the categories of tympanites, jaundice, liver stagnation, abdominal masses and the like in traditional Chinese medicine, the names of diseases in modern traditional Chinese medicine are collectively called liver cancer disease, and the liver cancer is divided into the following five symptoms: liver stagnation and spleen deficiency, damp-heat of liver and gallbladder, blood stasis due to liver heat, damp-encumbering due to spleen deficiency, and yin deficiency of liver and kidney. The main symptoms are qi and blood deficiency of viscera, spleen deficiency, dampness accumulation, phlegm coagulation and blood stasis; invasion of six excesses by pathogenic toxin, pathogenic coagulation and accumulation, seven emotions internal injury, emotional depression and the like cause qi, blood, dampness, heat, stasis and toxin to be combined to form liver cancer, and the main pathogenesis is that vital qi is deficient in the interior and pathogenic toxin is accumulated.

According to the common identification of the traditional Chinese medicine and western medicine for treating the liver cirrhosis, the liver cirrhosis is divided into 6 syndrome types: water-damp internal resistance type, spleen-kidney yang deficiency type, blood stasis collateral obstruction type, liver qi stagnation type, damp-heat accumulation type, and liver-kidney yin deficiency type.

Therefore, the traditional Chinese medicine with the effects of promoting blood circulation to remove blood stasis, strengthening spleen and replenishing qi, clearing heat and detoxicating, eliminating dampness and promoting diuresis, warming and invigorating spleen and kidney and the like is widely applied to clinical treatment of liver cancer and liver cirrhosis, and has obvious effect.

The invention relates to a traditional Chinese medicine composition, which consists of 21 traditional Chinese medicines. In the formula, the oldenlandia diffusa, the agrimony, the sculellaria barbata, the achyranthes bidentata, the areca peel, the combined spicebush root, the rhizoma atractylodis, the gardenia, the sweetgum fruit, the trogopterus dung-worm and the bighead atractylodes rhizome have the effects of clearing away heat and toxic materials, eliminating dampness and promoting diuresis, and warming and invigorating spleen and kidney; eupolyphaga Seu Steleophaga, Hirudo, squama Manis, endothelium corneum Gigeriae Galli, placenta hominis, radix Codonopsis, radix astragali, Poria, Notoginseng radix and radix Curcumae have effects of promoting blood circulation for removing blood stasis, invigorating spleen and invigorating qi. The compatibility of the medicine composition accords with the treatment rules of liver cancer and liver cirrhosis, and is mainly used for treating liver cancer and liver cirrhosis, particularly patients with advanced liver cancer and liver cirrhosis.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition with remarkable treatment effect on liver cancer and liver cirrhosis.

The traditional Chinese medicine composition for treating liver cancer and liver cirrhosis comprises the following traditional Chinese medicine raw materials in parts by weight: 90-100 parts of oldenlandia diffusa, 90-100 parts of hairyvein agrimony, 90-100 parts of sculellaria barbata, 45-55 parts of achyranthes bidentata, 45-55 parts of pericarpium arecae, 30-40 parts of radix linderae, 90-95 parts of rhizoma atractylodis, 30-35 parts of fructus gardeniae, 30-40 parts of fructus liquidambaris, 150-170 parts of radix aconiti szechenyiani, 90-105 parts of rhizoma atractylodis macrocephalae, 3-5 parts of ground beetle, 2-5 parts of leech, 2-4 parts of pangolin scales, 3-5 parts of endothelium corneum gigeriae galli, 4-5 parts of human placenta, 2-5 parts of codonopsis pilos.

According to the preferred scheme of the traditional Chinese medicine composition for treating liver cancer and liver cirrhosis, the weight parts of the raw materials are as follows: 90 parts of oldenlandia diffusa, 90 parts of hairyvein agrimony, 90 parts of barbed skullcap herb, 45 parts of twotooth achyranthes root, 45 parts of areca peel, 30 parts of combined spicebush root, 90 parts of swordlike atractylodes rhizome, 30 parts of cape jasmine fruit, 30 parts of beautiful sweetgum fruit, 150 parts of Chinese lobelia herb, 90 parts of largehead atractylodes rhizome, 3 parts of ground beetle, 2 parts of leech, 2 parts of pangolin scales, 3 parts of chicken's gizzard-membrane, 4.5 parts of human placenta, 3.

The traditional Chinese medicine composition for treating liver cancer and liver cirrhosis can be prepared into tablets, granules, dispersible tablets, capsules, pills, soft extracts, mixtures and water-honeyed pills.

The invention also provides a preparation method of the traditional Chinese medicine composition for treating liver cancer and liver cirrhosis.

The preparation method of the traditional Chinese medicine composition for treating liver cancer and liver cirrhosis comprises the following steps:

(1) crushing medicinal materials: pulverizing Eupolyphaga Seu Steleophaga, Hirudo, squama Manis, endothelium corneum Gigeriae Galli, placenta hominis, radix Codonopsis, radix astragali, Poria, Notoginseng radix and radix Curcumae into fine powder;

(2) the preparation method comprises the steps of taking oldenlandia diffusa, agrimony, barbed skullcap herb, twotooth achyranthes root, areca peel, combined spicebush root, rhizoma atractylodis, cape jasmine fruit, beautiful sweetgum fruit, five-claw dragon and largehead atractylodes rhizome according to the weight parts, adding water for 2 times, wherein the water amount added in each time is 12 times of the total weight of the medicinal materials, and the decocting time is 1.5 hours. Filtering, and concentrating the filtrate under reduced pressure to obtain fluid extract with relative density of 1.07-1.25 (measured at 25 deg.C);

(3) mixing the fine powder obtained in step (1) with the fluid extract obtained in step (2), drying under reduced pressure, pulverizing into fine powder, sieving, and making into corresponding dosage forms such as capsule, water-honeyed pill, soft extract, tablet and granule according to various dosage forms and adjuvants; or preparing the fine powder obtained in the step (1) into a solid preparation, and preparing the clear paste obtained in the step (2) into a liquid preparation, and taking the two preparations in a matching way.

Animal experiments and clinical application show that the traditional Chinese medicine composition for treating liver cancer and liver cirrhosis has an obvious anti-hepatic fibrosis effect; can obviously improve liver function and enhance the capability of the liver for resisting oxidative damage; can stabilize tumor body and inhibit liver cancer development; can improve the life quality of patients and obviously prolong the life cycle of patients.

Drawings

FIG. 1 Effect of Chinese medicinal composition on tumor size of H22 hepatoma bearing mice

FIG. 2 shows the effect of the Chinese medicinal composition on apoptosis-related protein in tumor tissue of H22 hepatoma bearing mice

(1) FIG. 2 is a protein band diagram in mouse tumor tissue, the deeper the protein band, the more the protein expression (based on the overall protein quantification, i.e. under the condition of consistent internal reference GAPDH band), and the measured indicators include: pro-apoptotic protein Bax, anti-apoptotic protein Bcl-2, cytochrome C (Cyt-C), Caspase3, and their activated forms, clear Caspase-3.

(2) The apoptosis-promoting protein Bax and the anti-apoptosis protein Bcl-2 can influence mitochondria to release cytochrome C (Cyt-C), and further activate Caspase family activation, so that the execution death factor Caspase3 is activated into cleared Caspase-3, and finally tumor cells are apoptotic.

The band of fig. 2 shows that the traditional Chinese medicine composition can improve the content of apoptosis-promoting protein Bax and cytochrome C protein, reduce the content of anti-apoptosis protein Bcl-2, increase the activation amount of cleared caspase-3, increase the apoptosis of tumor tissues by influencing the apoptosis pathway and inhibit the development of liver cancer.

Detailed Description

The present invention will be described in further detail with reference to specific examples, but the scope of the present invention as claimed is not limited to the following examples.

Example 1

A traditional Chinese medicine composition for treating liver cancer and liver cirrhosis is composed of the following components in parts by weight: 90 parts of oldenlandia diffusa, 90 parts of hairyvein agrimony, 90 parts of barbed skullcap herb, 45 parts of twotooth achyranthes root, 45 parts of areca peel, 30 parts of combined spicebush root, 90 parts of swordlike atractylodes rhizome, 30 parts of cape jasmine fruit, 30 parts of beautiful sweetgum fruit, 150 parts of Chinese lobelia herb, 90 parts of largehead atractylodes rhizome, 3 parts of ground beetle, 2 parts of leech, 2 parts of pangolin scales, 3 parts of chicken's gizzard-membrane, 4.5 parts of human placenta, 3.

Example 2

A traditional Chinese medicine composition for treating liver cancer and liver cirrhosis is composed of the following components in parts by weight: 95 parts of oldenlandia diffusa, 95 parts of hairyvein agrimony, 95 parts of barbed skullcap herb, 45 parts of twotooth achyranthes root, 45 parts of areca peel, 30 parts of combined spicebush root, 90 parts of rhizoma atractylodis, 30 parts of cape jasmine fruit, 30 parts of beautiful sweetgum fruit, 150 parts of dragon's blood, 95 parts of largehead atractylodes rhizome, 4 parts of ground beetle, 3 parts of leech, 3 parts of pangolin scales, 3.5 parts of chicken's gizzard-membrane, 4.5 parts of human placenta, 3 parts of pilose.

Example 3

Weighing 90 parts of spreading hedyotis herb, 90 parts of hairyvein agrimony, 90 parts of barbed skullcap herb, 45 parts of twotooth achyranthes root, 45 parts of areca peel, 30 parts of combined spicebush root, 90 parts of swordlike atractylodes rhizome, 30 parts of cape jasmine fruit, 30 parts of beautiful sweetgum fruit, 150 parts of five-claw dragon and 90 parts of largehead atractylodes rhizome. One dose is taken every day, 3 times a day, and the decoction is taken. Weighing 3 portions of Chinese polyphaga, 2 portions of leech, 2 portions of pangolin scales, 3 portions of chicken's gizzard-membrane, 4.5 portions of human placenta, 3 portions of radix codonopsitis, 3 portions of astragalus root, 3 portions of tuckahoe, 3 portions of notoginseng and 3 portions of radix curcumae 3 according to the weight portion, mixing and grinding the mixture into powder twice a day, and taking the powder with boiled water.

Example 4

A Chinese medicinal composition for treating hepatocarcinoma and liver cirrhosis is prepared into capsule and oral liquid by the following steps.

(1) According to the first weight part of the embodiment, the Chinese polyphaga, the leech, the pangolin scales, the endothelium corneum gigeriae galli, the human placenta, the codonopsis pilosula, the astragalus membranaceus, the poria cocos, the pseudo-ginseng and the radix curcumae are taken, crushed and sieved, so that the powder can completely pass through a 65-mesh sieve, the powder which can pass through a 80-mesh sieve is not more than 60 percent, the mixture is dried in a vacuum drying oven (-0.04 to-0.06 Mpa at 75 ℃) to constant weight, and the;

(2) according to the embodiment, according to the weight parts, oldenlandia diffusa, agrimony, sculellaria barbata, achyranthes bidentata, areca peel, combined spicebush root, rhizoma atractylodis, gardenia, beautiful sweetgum fruit, trogopterus dung and bighead atractylodes rhizome are taken, water is added for extraction for 2 times, the water amount added for each time is 12 times of the total mass of the medicinal materials, and the extraction time for each time is 1.5 hours. Filtering, combining filtrates, centrifuging to remove impurities, taking supernatant, concentrating under reduced pressure (60 ℃) to obtain clear paste with the relative density of 1.07-1.30 (measured at 25 ℃), cooling, adding 6% of aspartame, 0.25% of sodium benzoate and 0.05% of ethylparaben, stirring uniformly, filling and sterilizing to obtain the product.

Example 5

A Chinese medicinal composition for treating liver cancer and liver cirrhosis is prepared into tablets according to the following formula and steps:

(2) according to the second embodiment, the oldenlandia diffusa, the agrimony, the sculellaria barbata, the achyranthes bidentata, the areca peel, the combined spicebush root, the rhizoma atractylodis, the gardenia, the beautiful sweetgum fruit, the cairo glorybower herb and the bighead atractylodes rhizome are taken according to the parts by weight, water is added for 2 times, the water amount is 12 times of the total mass of the medicinal materials, and the extraction time is 1.5 hours. Filtering, combining filtrates, centrifuging to remove impurities, taking supernatant, and concentrating under reduced pressure to obtain fluid extract with relative density of 1.07-1.30 (measured at 25 ℃);

(3) mixing the fine powder obtained in step (1) with the fluid extract obtained in step (2), drying under reduced pressure, pulverizing into fine powder, sieving, adding appropriate adjuvant, granulating, and tabletting.

Example 6

A Chinese medicinal composition for treating hepatocarcinoma and liver cirrhosis can be made into other dosage forms.

For example, after mixing the fine powder obtained in the step (1) and the clear paste obtained in the step (2) in the example 5, drying under reduced pressure, adding proper auxiliary materials for granulation, and obtaining granules; or mixing the fine powder obtained in the step (1) and the clear paste obtained in the step (2) in the embodiment 5, and adding refined honey or sugar to obtain the soft extract.

Example 7

Experiment on treatment effect of traditional Chinese medicine composition on experimental liver cirrhosis rats

Materials (I) and (II)

1. Medicine preparation: the drug was formulated as in example 1.

2. Animals: SD rats, SPF grade, male, body weight (200 ± 20) g, provided by the animal testing center of the university of pharmaceutical, guangxi.

Second, Experimental methods

Selecting 72 SPF male SD rats, randomly selecting 11 rats, adding into solvent control group, and administering equal volume of purified water, wherein the volume of the rest rats is 2 mL/kg^-1Intragastric perfusion of 50% CCl₄Olive oil was used 2 times a week for 8 weeks to establish a cirrhosis model. The 55 model rats were randomly divided into 5 groups, model group, drug low, medium and high dose groups (5.4, 10.8, 21.5g crude drug/kg)^-1) Carapax Trionycis liver softening tablet set (0.8g kg)^-1) 11 per group. According to 10 mL/kg^-1Administration phase by intragastric administrationThe liquid medicine, the solvent group and the model group are administrated with purified water with equal volume, and the administration is performed for 4 weeks after intragastric administration for 1 time respectively in the morning and afternoon of a day. Before the last administration, fasting is carried out for 12 h-16 h without water prohibition, and after the last administration, the dose is 3 mL/kg^-1Intraperitoneal injection of 10% chloral hydrate for anesthesia, abdominal aorta blood sampling, 4000 rmp.min^-1Centrifuging for 10min, collecting serum to measure liver function index and hepatic fibrosis index, and collecting liver tissue of the same part to measure antioxidant capacity index levels such as SOD and MDA.

Third, experimental results

The results are shown in tables 1, 2 and 3.

TABLE 1 Chinese medicinal composition for CCl₄Effect of liver function in rats causing liver cirrhosis

Note: in comparison with the set of models,¹⁾P＜0.05，²⁾P＜0.01。

TABLE 2 Chinese medicinal composition for CCl₄Effect of hepatic fibrosis level in rats with liver cirrhosis

Note: in comparison with the set of models,¹⁾P＜0.05，²⁾P＜0.01。

TABLE 3 Chinese medicinal composition for CCl₄Influence of liver tissue antioxidant index of rat with liver cirrhosis

Note: in comparison with the set of models,¹⁾P＜0.05，²⁾P＜0.01。

the experimental results show that the traditional Chinese medicine composition can obviously improve the liver function of experimental liver cirrhosis rats, enhance the capability of the liver for resisting fibrosis formation and improve the oxidation resistance of the liver.

Example 8

Anti-tumor effect experiment of traditional Chinese medicine composition on mouse H22 liver cancer transplantation tumor

Materials (I) and (II)

1. Medicine preparation: the drug was formulated as in example 2.

2. Animals: KM mice, 22-25 g, male, were provided by the laboratory animal center of Guangxi medical university.

3. Tumor strain: provided by the pharmacological research laboratory at the university of medical science, Guangxi and subcultured.

Second, Experimental methods

Killing mice inoculated with H22 ascites tumor with obvious abdominal bulge for 9 days by cervical dislocation, extracting tumor liquid from abdominal cavity under aseptic condition, diluting with aseptic normal saline, and 800 rmp.min^-1Centrifuging for 5min, removing supernatant to obtain tumor cells, and adding physiological saline to adjust tumor cell concentration to 1 × 10⁷Mice were inoculated subcutaneously in the right axilla at this concentration with tumor cell suspension at 0.2 mL/mL (except for the normal group). Weighing 60 mice 24 hours after inoculation, and inoculating 10 non-inoculated mice into a normal group; the remaining 50 were randomly divided into 5 groups: the medicine has low, medium and high dosage (13.9, 27.7, 55.4g crude drug/kg)^-1) Group (d); cyclophosphamide group (20mg kg)^-1) And model control groups of 10 animals each. The dosage of the composition is 20 ml/kg^-1Performing intragastric administration in volume, and performing once every morning and afternoon; equal volume of the normal saline is used for the model control group; the cyclophosphamide group is administered by intraperitoneal injection with a dosage volume of 20m1 kg^-1Every two days. After continuous administration for 10 days, blood is taken from eyeballs for killing, thymus, spleen and tumor are picked, weight is weighed, and tumor inhibition rate, spleen index and thymus index are calculated. Tumor inhibition rate ═ (mean tumor weight model group-mean tumor weight administered group)/mean tumor weight model group × 100%; spleen or thymus index-spleen (thymus) weight mg/body weight g × 10.

Third, experimental results

The results are shown in tables 4 and 5.

TABLE 4 influence of the Chinese medicinal composition on the body weight, thymus index and spleen index of H22 hepatocarcinoma tumor-bearing mice

Note: in comparison with the set of models,¹⁾P＜0.05，²⁾P＜0.01。

TABLE 5 influence of the Chinese medicinal composition on tumor weight and tumor inhibition rate of mouse liver cancer transplantable tumor

Note: in comparison with the set of models,¹⁾P＜0.05，²⁾P＜0.01。

the result shows that the tumor inhibition rate of the traditional Chinese medicine composition administration group to the H22 liver cancer transplantation tumor can reach 47.39%, which shows that the traditional Chinese medicine composition has obvious inhibition effect on experimental liver cancer (see the attached figure 1 of the specification).

In addition, indexes of apoptosis-promoting protein Bax, anti-apoptosis protein Bcl-2, cytochrome C (Cyt-C), Caspase3 and activated form thereof, such as cleaned Caspase-3, in tumor tissues are measured, and results show that the traditional Chinese medicine composition can improve the contents of the apoptosis-promoting protein Bax and the cytochrome C protein, reduce the content of the anti-apoptosis protein Bcl-2, increase the activated amount of the cleaned Caspase-3, increase tumor tissue apoptosis by influencing an apoptosis pathway and inhibit the development of liver cancer (see the attached figure 2 of the specification).

Example 9

Patients of Huangxxx, male, 58 years old, Guangxi Nanning people, liver and gall surgery diagnosis of tumor hospital of Guangxi medical university shows that liver cancer is late with ascites due to cirrhosis, and the life cycle is predicted to be 6 months. After the patient abandons the treatment and is discharged from hospital, the patient takes the traditional Chinese medicine composition of the third embodiment to treat the disease. After the medicine is taken for half a year, the re-examination finds that the hepatoma tumor mass is reduced, the ascites is eliminated and the liver function is gradually improved. The patient also returned from the initial bedridden condition to simple housework. After the medicine is continuously taken for half a year, the daily life of the patient is recovered, and simple agricultural labor can be carried out. The proved recipe prolongs the life of the patient by 2 years.

Patients with Liu XXx, male and 48 years old suffer from liver cirrhosis, dry and rough skin, gray and dark complexion, vomiting and diarrhea, even bedridden, have improved spirit after half a month of continuous treatment, obviously inhibit the state of the illness after 3 months, do daily housework, and normally live at diet and normally carry out agricultural labor during the period of continuing taking the medicine.

In conclusion, the traditional Chinese medicine composition for treating liver cancer and liver cirrhosis has obvious effects of resisting liver fibrosis formation, improving liver functions and enhancing the capacity of resisting oxidative damage of the liver; can stabilize tumor body, promote tumor tissue apoptosis, and inhibit liver cancer development; improve the life quality of the patients and obviously prolong the life cycle of the patients.

Claims

1. A traditional Chinese medicine composition for treating liver cancer and liver cirrhosis is composed of the following 21 traditional Chinese medicines in parts by weight: 90-100 parts of oldenlandia diffusa, 90-100 parts of hairyvein agrimony, 90-100 parts of sculellaria barbata, 45-55 parts of achyranthes bidentata, 45-55 parts of pericarpium arecae, 30-40 parts of radix linderae, 90-95 parts of rhizoma atractylodis, 30-35 parts of fructus gardeniae, 30-40 parts of fructus liquidambaris, 150-170 parts of radix aconiti szechenyiani, 90-105 parts of rhizoma atractylodis macrocephalae, 3-5 parts of ground beetle, 2-5 parts of leech, 2-4 parts of pangolin scales, 3-5 parts of endothelium corneum gigeriae galli, 4-5 parts of human placenta, 2-5 parts of codonopsis pilos.

2. The traditional Chinese medicine composition for treating liver cancer and liver cirrhosis according to claim 1, wherein the traditional Chinese medicine comprises the following raw materials in parts by weight: 90 parts of oldenlandia diffusa, 90 parts of hairyvein agrimony, 90 parts of barbed skullcap herb, 45 parts of twotooth achyranthes root, 45 parts of areca peel, 30 parts of combined spicebush root, 90 parts of swordlike atractylodes rhizome, 30 parts of cape jasmine fruit, 30 parts of beautiful sweetgum fruit, 150 parts of Chinese lobelia herb, 90 parts of largehead atractylodes rhizome, 3 parts of ground beetle, 2 parts of leech, 2 parts of pangolin scales, 3 parts of chicken's gizzard-membrane, 4.5 parts of human placenta, 3.

3. The Chinese medicinal composition for treating liver cancer and liver cirrhosis according to claim 1, which is prepared into tablets, granules, capsules, pills, soft extract or mixture.

4. The preparation method of the Chinese medicinal composition for treating liver cancer and liver cirrhosis according to claim 2, comprising the following steps:

(2) taking oldenlandia diffusa, hairyvein agrimony, barbed skullcap herb, twotooth achyranthes root, areca peel, combined spicebush root, swordlike atractylodes rhizome, cape jasmine fruit, beautiful sweetgum fruit, five-claw dragon and largehead atractylodes rhizome according to the parts by weight, adding water for 2 times, wherein the water amount is 12 times of the total mass of the medicinal materials each time, the decocting time is 1.5 hours each time, filtering, and concentrating the filtrate under reduced pressure to obtain clear paste with the relative density of 1.07-1.25 at 25 ℃;

(3) mixing the fine powder obtained in step (1) and the fluid extract obtained in step (2), drying under reduced pressure, pulverizing into fine powder, sieving, and making into capsule, pill, soft extract, tablet or granule according to dosage form process and adjuvant requirement.