CN108718839A - A kind of application of amblyseius cucumeris oudeman in control strawberry Tetranychus urticae or in improving strawberry quality - Google Patents
A kind of application of amblyseius cucumeris oudeman in control strawberry Tetranychus urticae or in improving strawberry quality Download PDFInfo
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- CN108718839A CN108718839A CN201810537622.6A CN201810537622A CN108718839A CN 108718839 A CN108718839 A CN 108718839A CN 201810537622 A CN201810537622 A CN 201810537622A CN 108718839 A CN108718839 A CN 108718839A
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- strawberry
- amblyseius cucumeris
- cucumeris oudeman
- tetranychus urticae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of application of amblyseius cucumeris oudeman in control strawberry Tetranychus urticae or in improving strawberry quality.The application includes discharging amblyseius cucumeris oudeman on strawberry.The blank of " control effect that amblyseius cucumeris oudeman controls strawberry Tetranychus urticae " and " being influenced on strawberry quality after release amblyseius cucumeris oudeman " assay method has been filled up in application provided by the invention, quantitative data supporting is provided to its control effect and strawberry quality simultaneously, is more advantageous on producing and is widely applied.
Description
Technical field
The invention belongs to strawberry harmful organism green prevention and control technical fields, and in particular to a kind of amblyseius cucumeris oudeman is careless in control
Application in certain kind of berries Tetranychus urticae or raising strawberry quality.
Background technology
Red spider category Arachnoidea Acarina Tetranychidae, has generation in Chinese Strawberry Industry producing region.Cause harm the red spider of Chinese Strawberry Industry
Spider is mainly Tetranychus urticae, is one of the important pests on strawberry.It is often gathered in blade back absorption juice to cause harm, spins and tie on blade face
Net, initial stage white dot；When insect density is big, plant is aggrieved serious, and face of blade edge is rubescent withered in rust, and shape is like fire
It burns, plant strain growth is suppressed, seriously affects the yield and quality of strawberry.
The upper most common Prevention Technique of production at present is still Agro-chemicals control.However, due to unreasonably using for a long time
Pesticide so that Tetranychus urticae produces height drug resistance or drug resistance to a variety of Insecticidal and acaricidal agents, and control effect cannot be effective
Ensure.Meanwhile consumer increasingly pays close attention to strawberry edible safety problem, it is desirable that the producer more pays close attention to the green of Tetranychus urticae
Control Technology.Biological control method is increasingly taken seriously as a kind of green prevention and control means for producing nuisanceless fruit and vegetable food.
Amblyseius cucumeris oudeman category Phytoseiidae Amblysiuss are using one of more successful predator in biological control, both at home and abroad
It has been successfully applied to the prevention of the harmful organisms such as thrips, mite class and Bemisia tabaci on other crops, significant effect.But it is blunt using cucumber
The pest control effect of peaceful acarid control strawberry Tetranychus urticae and the influence to strawberry quality are studied and are but had not been reported.
Main cause one is that strawberry Tetranychus urticae build is smaller, and ovum, young mite and deutonymph are generally regarded in magnifying glass or insect bodies
Under mirror just it is observed that, when field orchard worker has found that it is endangered, tend to occur than more serious, general biological control means are difficult to solve
Root problem, therefore, chemical prevention means become orchard worker's prefered method；Second is that strawberry Tetranychus urticae harmful characteristics are from point to surface
It is extended, if do not controlled in " point " stage of development, until " face " occurs, is controlled with the method for release Predatory Mites,
Effect can be very poor；Third, a few studies personnel have " interference " reaction, amblyseius cucumeris oudeman to the discovery of amblyseius cucumeris oudeman desk research
With evil mite benefit evil than being more than certain proportion, can decline with the increase of burst size, control effect.Therefore, field large area pushes away
Extensively also it is in the exploratory stage.Fourth, currently with Predatory Mites control strawberry Tetranychus urticae control effect define only rest on it is qualitative
In the stage, production is upper apparent in Tetranychus urticae early period of origination releasing effect, but lacks quantitative data and support；Fifth, the producer and consumption
Just " harmfulness of the Tetranychus urticae to strawberry production " is actually also scarce whether there is or not influencing on the quality of strawberry although there is common recognition by person
Few data supporting.
Therefore, letter amblyseius cucumeris oudeman to be carried out is to the field control action of Tetranychus urticae on facility cultivation strawberry and its to grass
The influence of certain kind of berries quality is studied, to provide technical basis for strawberry green production.
Invention content
Goal of the invention：In order to solve the above-mentioned problems in the prior art, the present invention provides a kind of amblyseius cucumeris oudemans
Application in control strawberry Tetranychus urticae or in improving strawberry quality.
Technical solution：Amblyseius cucumeris oudeman of the present invention answering in control strawberry Tetranychus urticae or raising strawberry quality
With.
The application includes discharging amblyseius cucumeris oudeman on strawberry.
Preferably, the burst size of amblyseius cucumeris oudeman is every plant of strawberry 150-300 head.
The amblyseius cucumeris oudeman can improve strawberry hardness.
The amblyseius cucumeris oudeman can improve strawberry Vitamin C content.
The amblyseius cucumeris oudeman can improve Content of Soluble Solid Substance of Strawberry.
The amblyseius cucumeris oudeman can reduce titratable acid content.
The amblyseius cucumeris oudeman can improve the ratio of soluble solid and titratable acid content.
Advantageous effect：
1, a kind of release amblyseius cucumeris oudeman of the invention control strawberry Tetranychus urticae and the method for improving strawberry quality are filled up
" amblyseius cucumeris oudeman controls the control effect of strawberry Tetranychus urticae " and " strawberry quality is influenced after release amblyseius cucumeris oudeman " survey
Determine the blank of method, while quantitative data supporting is provided to its control effect and strawberry quality, is more advantageous to life
It is widely applied in production.
2, the control method that the present invention uses is biological control, belongs to the scope of harmful organism green prevention and control, simple and practicable,
Easily operated, after implementing in production, non agricultural chemical residuum is environmentally safe, to consumer's edible safety, can effectively reduce strawberry
The usage amount of chemical pesticide in production, while effectively improving the quality of strawberry and improving the commodity value of strawberry, for grass
The green production of the certain kind of berries escorts.
3, the method for the present invention is simple and easy to operate, and blade and fruit demand are limited in experiment, test result data stabilization, weight
Renaturation is strong, can meet researcher, Predatory Mites manufacturing enterprise investigative technique demand；Simultaneously to the control effect of Tetranychus urticae
There is quantitative analysis with to the raising of strawberry quality, data are full and accurate, and technical support and theory are provided to be widely applied in production
Support.
Specific implementation mode
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
It applies content described in example and is merely to illustrate the present invention, without sheet described in detail in claims should will not be limited
Invention.
Embodiment 1
1, test material
Strawberry cultivars：Peach is smoked, is colonized in part of in September, 2017, strawberry is in flowering fruit bearing stage during experiment.
Tetranychus urticae：Naturally-occurring in the greenhouse of the gardens Jiangsu Polytechnic College of Agriculture and Forestry Nong Bo, host plant are strawberry.
Amblyseius cucumeris oudeman：It is produced by Fujian Yan Xuan biological preventions Co., Ltd, it is bottled, 50,000 every bottle.
2, experimental method
Strawberry heliogreenhouse 1, area 600m2, high-level cultivating.Plant 1200 plants of strawberry (30 row × 40 plant).Experiment is altogether
If 4 processing：1. amblyseius cucumeris oudeman burst size is 150 heads/plant；2. amblyseius cucumeris oudeman burst size is 200 heads/plant；3. cucumber is blunt
Mite burst size of pacifying is 300 heads/plant；4. blank control does not discharge Phyloseiulus nersimilis.Often handle 3 repetitions, 3 processing totally 12
A cell, each cell handle random alignment, per 2 row of cell, 80 plants.March 2 2018 time on-test.
3, control effect is investigated
The mite mouthful radix of Tetranychus urticae is first investigated before March 2 test process, 7d, 14d, 21d divide again after discharging Predatory Mites
Tiao Cha not be 1 time, it investigates 4 times altogether.Per cell 5 plants of strawberries of random searching, every plant of upper, middle and lower takes 1 leaf, investigates Tetranychus urticae mite
Mouth number.Strawberry Leaves are collected in hermetic bag, every leaf is individually put, and laboratory is taken back, and every is observed and recorded under stereoscope
The quantity of Tetranychus urticae on leaf calculates Revision insect recluced rate and preventive effect (be calculated at deutonymph, young mite mite mouthful number).
4, fruit quality measures
The index of quality such as VC, soluble solid, organic acid are measured in fructescence.
4.1 hardness
Hardness is measured using GY-2 type fruit hardness meters.5 fruits, each fruit are taken to measure negative and positive respectively at random per cell
Flushing road position, is repeated 5 times.
4.2 soluble solids (TSS) content
Refractometer is held using BX-1 to measure.5 fruits are taken at random per cell, select fresh strawberry 5g or so respectively,
It is to be determined at being homogenized with mortar grinder, it is careful not to keep temperature excessively high in process of lapping.
4.3VC assay
It is measured using 2,6- dichloroindophenols.Each processing weighs 10g pulp, is put into mortar plus 2% oxalic acid solution is ground a little
It is broken, it injects in 200ml volumetric flasks, adds 2% oxalic acid to be diluted to scale, filtrate is collected by filtration, be measured with 2,6- dichloroindophenols.
4.4 titratable acids (TA) content
Using NaOH titrations.Each processing weighs 10g pulp, and homogenate is broken into homogenizer, and 10mL distilled water, homogenate is added
Distilled water is settled to 250mL, and filtrate is collected by filtration, and is titrated with standard NaOH solution, is as a result indicated with citric acid percentage.
4.5 data analysis
Test data Excel 2016 and SPSS17.0 processing analyze significant difference using DuncanShi duncan's new multiple range methods
Property.The Rectifying efficacies respectively handled calculate acquisition according to the following formula：
Revision insect recluced rate (%)=[insect population number before (insect population number after insect population number-processing before processing)/processing] × 100
Control effect (%)=[(treatment region Revision insect recluced rate-check plot Revision insect recluced rate)/(check plots 100- insect population subtracts
Move back rate)] × 100
5 experimental results
Control effect of 5.1 different disposals to strawberry Tetranychus urticae
Table 1 discharges control effect of the Predatory Mites to strawberry Tetranychus urticae
1) significant difference (P < 0.05) between different lowercase letters are respectively handled with after column data in table
As seen from Table 1,3 processing have certain inhibiting effect to the total mite amount of strawberry Tetranychus urticae, to discharge predation
300 heads/plant of preventive effect highests of mite, release 200 heads/plant of effects of Predatory Mites are taken second place, and release 150 heads/plant of effects of Predatory Mites are worst.
7d, 14d and 21d after prevention, 3 processing preventive effect differences are notable.7d after prevention, 300 heads/plant of release Predatory Mites are anti-
Highest is imitated, is 87.04%, it is notable with other 2 processing differences；14d after prevention, release 300 heads/plant of preventive effects of Predatory Mites reach
99.04%, it is significantly higher than other 2 processing, the processing than discharging 150 heads/plant of Predatory Mites is 39.49 percentage points high；After prevention
21d, 3 processing preventive effects reach maximum correction preventive effect, and release 300 heads/plant of preventive effects of Predatory Mites reach 99.91%, release predation
150 heads/plant of preventive effects of mite also reach 76.18%.
200 heads/plant of release initial stages of 150 heads/plant of Predatory Mites and release Predatory Mites are discharged, mite amount is in rising trend, hereafter mite
Amount slowly declines, and after preventing 21d, mite amount declines the most apparent.Predatory Mites 300 heads/plant of 7d, 14d and 21d are discharged, mite amount is always
In stable low-level, after preventing 14d, field mite amount declines the most apparent；After preventing 21d, field is difficult to look into see two variegated leafs
Mite.It can be seen that the effect for increasing Predatory Mites burst size is fairly obvious, playing significant inhibition to strawberry Tetranychus urticae makees
With, it is handled relative to other two, it is more stable to the control effect of Tetranychus urticae, lasting.
Influence of 5.2 different disposals to strawberry quality
2 different disposal strawberry quality index of table
2) significant difference (P < 0.05) between different lowercase letters are respectively handled with after column data in table
As seen from Table 2, release Predatory Mites respectively processing on strawberry hardness influence significant difference, with discharge Predatory Mites 300/
Strain hardness is maximum, relatively compares hardness and increases by 67.59 percentage points；Release Predatory Mites Content of Soluble Solid Substance of Strawberry respectively handles difference
It is different not notable, but soluble solid content relatively compares high 1.32%, 6.88% and 18.73% respectively；Titratable acid content is empty
White control is 0.52%, is significantly higher than release Predatory Mites and respectively handles, wherein 300 heads/plant of titratable acid contents of release Predatory Mites are most
Low, relatively control reduces 14.5 percentage points；It is not notable on the influence of strawberry VC contents to discharge Predatory Mites, wherein release Predatory Mites 200
Head/plant and 300 heads/plant be respectively 8.5mg and 9.1mg, relatively control improve 5.88% and 12.09%.
In gardening, usually fruit is evaluated with soluble solid and the ratio (referred to as solid sour ratio) of titratable acid content
Taste of fruit and maturity, Gu sour ratio is bigger, the flavor of fruit is better[6,7].Admittedly acid ratio is respectively 3 processing of release Predatory Mites
9.64,10.91 and 13.75, high 10.89 percentage points, 21.26 percentage points and 37.53 percentage points are relatively compareed respectively, wherein
It is significantly higher than control to discharge the solid sour ratio of 300 heads/plant of Predatory Mites.It can be seen that strawberry flavor obtains obviously after release Predatory Mites
It improves.
It to sum up analyzes, 3 processing each indexs of strawberry quality of release Predatory Mites have different compared with the control, with release
Each index performance of 300 heads/plant of Predatory Mites is the most apparent, and release 200 heads/plant of performances of Predatory Mites are taken second place, and release Predatory Mites 150/
Strain performance is worst.By discharging Predatory Mites strawberry hardness, VC contents, soluble solid and solid acid ratio, relatively control increases, can
It is obviously improved the quality of strawberry, improves strawberry fruit flavor.
Claims (8)
1. a kind of application of amblyseius cucumeris oudeman in control strawberry Tetranychus urticae or in improving strawberry quality.
2. application according to claim 1, which is characterized in that including discharging amblyseius cucumeris oudeman on strawberry.
3. application according to claim 2, which is characterized in that the burst size of amblyseius cucumeris oudeman is every plant of strawberry 150-300
Head.
4. according to claim 1-3 any one of them applications, which is characterized in that it is hard that the amblyseius cucumeris oudeman can improve strawberry
Degree.
5. according to claim 1-3 any one of them applications, which is characterized in that the amblyseius cucumeris oudeman can improve strawberry dimension
Raw element C content.
6. according to claim 1-3 any one of them applications, which is characterized in that the amblyseius cucumeris oudeman can improve strawberry can
Dissolubility solid content.
7. according to claim 1-3 any one of them applications, which is characterized in that the amblyseius cucumeris oudeman can reduce titratable
Acid content.
8. according to claim 1-3 any one of them applications, which is characterized in that the amblyseius cucumeris oudeman can improve solubility
The ratio of solid content and titratable acid content.
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| CN201810537622.6A CN108718839A (en) | 2018-05-29 | 2018-05-29 | A kind of application of amblyseius cucumeris oudeman in control strawberry Tetranychus urticae or in improving strawberry quality |
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| CN201810537622.6A CN108718839A (en) | 2018-05-29 | 2018-05-29 | A kind of application of amblyseius cucumeris oudeman in control strawberry Tetranychus urticae or in improving strawberry quality |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1440657A (en) * | 2002-02-24 | 2003-09-10 | 张艳璇 | Orange pest mite preventing and killing method using cucumber amblyseius as predatory mite |
| CN1480046A (en) * | 2002-09-06 | 2004-03-10 | 张艳璇 | New method and usage for preventing and treating harm acarid in bamboo forest by using Amblyseium cucumeris to prey on acarid |
| CN1729792A (en) * | 2004-08-13 | 2006-02-08 | 张艳璇 | New purpose for controlling harmful cotton spider mites by utilizing amblyseius cucumeris as biological pesticide |
| CN104488848A (en) * | 2014-11-26 | 2015-04-08 | 曾少兰 | New method and application for controlling red spiders of Shatian pomelo by amblyseius cucumeris |
| CN105746254A (en) * | 2016-03-14 | 2016-07-13 | 福建艳璇生物防治技术有限公司 | Method for preventing red spiders, thrips and aleyrodids on strawberries by amblyseius cucumeris |
-
2018
- 2018-05-29 CN CN201810537622.6A patent/CN108718839A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1440657A (en) * | 2002-02-24 | 2003-09-10 | 张艳璇 | Orange pest mite preventing and killing method using cucumber amblyseius as predatory mite |
| CN1480046A (en) * | 2002-09-06 | 2004-03-10 | 张艳璇 | New method and usage for preventing and treating harm acarid in bamboo forest by using Amblyseium cucumeris to prey on acarid |
| CN1729792A (en) * | 2004-08-13 | 2006-02-08 | 张艳璇 | New purpose for controlling harmful cotton spider mites by utilizing amblyseius cucumeris as biological pesticide |
| CN104488848A (en) * | 2014-11-26 | 2015-04-08 | 曾少兰 | New method and application for controlling red spiders of Shatian pomelo by amblyseius cucumeris |
| CN105746254A (en) * | 2016-03-14 | 2016-07-13 | 福建艳璇生物防治技术有限公司 | Method for preventing red spiders, thrips and aleyrodids on strawberries by amblyseius cucumeris |
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Application publication date: 20181102 |