CN108715808A - A kind of device for growth of tumour cell Inhibition test - Google Patents
A kind of device for growth of tumour cell Inhibition test Download PDFInfo
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- CN108715808A CN108715808A CN201810571505.1A CN201810571505A CN108715808A CN 108715808 A CN108715808 A CN 108715808A CN 201810571505 A CN201810571505 A CN 201810571505A CN 108715808 A CN108715808 A CN 108715808A
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- growth
- tumour cell
- inhibition test
- babinet
- cell inhibition
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
It is disclosed by the invention to belong to tumor suppression experimental technique field, a kind of specially device for growth of tumour cell Inhibition test, including babinet, uniformly there are three partition boards for longitudinal grafting for the inner cavity of the babinet, the partition board is fixedly connected with the inner cavity of babinet by screw, babinet is divided into four unit experiment rooms by three partition boards, it is hinged on front side of the unit experiment room and picks and places door, laterally straight slot is offered in the middle part of the antetheca for picking and placeing door, and straight slot has been internally embedded viewing window, the top of the unit experiment room is embedded with head cover, size by controlling electric current controls the power of electric heating tube, it can be suitable for a variety of different temperature heating and thermal insulation modes；It can change air inflow by the adjustable mode of charge flow rate；By the setting of rubber stopper, experimental pharmacy can be injected to experimental ware in real time by syringe；By the setting of viewing window, internal experimental conditions can be observed in real time, and do not influence its normal experiment.
Description
Technical field
The present invention relates to tumor suppression experimental technique fields, specially a kind of dress for growth of tumour cell Inhibition test
It sets.
Background technology
In antitumor research, there has been proposed tumour multi-step, DNA mutation, Apoptosis, cell cycle core engines
Many theoretical and theories such as system, cell cycle start and a plurality of signal transduction pathway participates in.
The research and development of antitumor drug with theoretical update and technology progress, from the killing for target spot with nucleic acid etc.
Hinder research of the type Cytotoxic drugs steering to tumour novel targets.Including chemoprophylactic drug, differentiating inducer, inducer of apoptosis, signal
Block agent, angiogenesis inhibitors, biological regulator etc..
In antitumor developmental research, detection drug is one important to the experimental study that growth of tumour cell inhibits
Research project.Existing generally use dye exclusion method detection drug inhibits growth of tumour cell.
In existing laboratory apparatus, no special whole device mating for growth of tumour cell Inhibition test,
When being tested, gas concentration lwevel and temperature can not be efficiently controlled so that tumour cell cannot when by medicine culture
It gets a desired effect, and then influences the result evaluation entirely tested, it is low so as to cause experiment precision.
Invention content
The purpose of the present invention is to provide a kind of devices for growth of tumour cell Inhibition test, to solve above-mentioned background
In the existing laboratory apparatus proposed in technology, without the special whole dress mating for growth of tumour cell Inhibition test
Set, when being tested, gas concentration lwevel and temperature can not be efficiently controlled so that tumour cell when by medicine culture not
The problem of capable of getting a desired effect.
To achieve the above object, the present invention provides the following technical solutions：It is a kind of for growth of tumour cell Inhibition test
Device, including babinet, uniformly there are three partition board, the inner cavity of the partition board and babinet passes through spiral shell for longitudinal grafting for the inner cavity of the babinet
Nail is fixedly connected, and babinet is divided into four unit experiment rooms by three partition boards, is hinged on front side of the unit experiment room
Door is picked and placeed, laterally offers straight slot in the middle part of the antetheca for picking and placeing door, and straight slot has been internally embedded viewing window, the unit
The top in laboratory is embedded with head cover, is embedded with rubber stopper in the middle part of the head cover, the left side of the head cover be longitudinally plugged with into
Tracheae is equipped with flow control valve on the pipeline of the air inlet pipe, and the flow control valve is located at the upside of head cover, the head cover
Right side be longitudinally plugged with escape pipe, the rubber stopper is between air inlet pipe, escape pipe, the bottom card of the unit experiment room
It is connected to current regulator, the inner wall of the unit experiment room is bonded with incubation cavity by bonding agent, and the inner wall of the incubation cavity is logical
It crosses buckle circumferential direction and is connected with electric heating tube, the electric heating tube is electrically connected with current regulator, the interior bottom of chamber of the incubation cavity
It is connected by screw column in the middle part of portion, the top of the column is welded with material containing disk, and the top braces of material containing disk have experiment
Vessel.
Preferably, the height of the viewing window and the height of experimental ware are identical.
Preferably, the dimensional fits mode between the head cover and rubber stopper is interference fit.
Preferably, the bottom end of the air inlet pipe stretches in the middle part of the inner cavity of experimental ware.
Preferably, the bottom end of the escape pipe is connected with flaring gas gathering mask.
Preferably, it is embedded with dustproof sponge plug on the upside of the inner cavity of the escape pipe.
Preferably, the current regulator is sliding rheostat current regulator.
Preferably, the incubation cavity is calcium silicate heat-preserving chamber.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) power that electric heating tube is controlled by controlling the size of electric current can be suitable for a variety of different temperature heating
Heat preserving mode；
(2) air inflow can be changed by the adjustable mode of charge flow rate；
(3) by the setting of rubber stopper, experimental pharmacy can be injected to experimental ware in real time by syringe；
(4) by the setting of viewing window, internal experimental conditions can be observed in real time, and it is normal real not influence it
It tests.
Description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is the schematic cross-sectional view of Fig. 1 of the present invention.
In figure：1 babinet, 2 partition boards, 3 pick and place door, 4 viewing windows, 5 head covers, 6 rubber stoppers, 7 air inlet pipe, 8 flow controls
Valve, 9 escape pipes, 10 current regulators, 11 electric heating tubes, 12 incubation cavities, 13 columns, 14 experimental wares.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
- 2 are please referred to Fig.1, the present invention provides a kind of technical solution：A kind of dress for growth of tumour cell Inhibition test
It sets, including babinet 1, uniformly there are three partition board 2, partition board 2 and the inner cavity of babinet 1 are solid by screw for longitudinal grafting for the inner cavity of babinet 1
Babinet 1 is divided into four unit experiment rooms, is hinged on front side of unit experiment room and picks and places door 3, taken by fixed connection, three partition boards 2
It puts and laterally offers straight slot in the middle part of the antetheca of door 3, and straight slot has been internally embedded viewing window 4, the top of unit experiment room is embedding
Enter to have head cover 5, the middle part of head cover 5 to be embedded with rubber stopper 6, the left side of head cover 5 is longitudinally plugged with air inlet pipe 7, the pipeline of air inlet pipe 7
On flow control valve 8 is installed, flow control valve 8 is located at the upside of head cover 5, and the right side of head cover 5 is longitudinally plugged with escape pipe 9,
For rubber stopper 6 between air inlet pipe 7, escape pipe 9, the bottom of unit experiment room is connected with current regulator 10, unit experiment room
Inner wall incubation cavity 12 is bonded with by bonding agent, the inner wall of incubation cavity 12 is connected with electric heating tube 11 by buckling circumferential direction, electricity
Heating tube 11 is electrically connected with current regulator 10, and column 13 has been connected by screw in the middle part of the intracavity bottom of incubation cavity 12, is stood
The top of column 13 is welded with material containing disk, and the top braces of material containing disk have experimental ware 14.
Wherein, the height of viewing window 4 is identical as the height of experimental ware 14, the ruler between head cover 5 and rubber stopper 6
Very little fit system is interference fit, and the bottom end of air inlet pipe 7 stretches in the middle part of the inner cavity of experimental ware 14, and the bottom end of escape pipe 9 connects
It is connected to flaring gas gathering mask, dustproof sponge plug is embedded on the upside of the inner cavity of escape pipe 9, current regulator 10 is sliding rheostat electric current
Modulator, incubation cavity 12 are calcium silicate heat-preserving chamber.
The dominant mechanism of this programme quantity method in order to control, by the situation of change of the homoreactant under different condition to anti-
The living condition of object is answered to judge, the variable of this programme is mainly temperature, the ingredient of gas and content, chemical reagent；
Four unit experiment rooms are independent individual, enter the two of corresponding units laboratory by the regulation and control of flow control valve 8
The concentration of carbonoxide, electric heating tube 11 use Resistant heating mode, and current regulator 10 is connected with electric heating tube 11, by changing
The resistance value of time-dependent current modulator 10 and change total resistance value on 11 circuit of electric heating tube, to change the heating work(of electric heating tube 11
Rate；
Four unit experiment rooms from left to right be respectively control group, the first experimental group, the second experimental group and third experimental group,
Tumour cell is cultivated after by medicine under certain gas concentration lwevel and temperature condition, will be swollen after culture after culture
Oncocyte is divided into the similar tumor cell group of growth conditions, is divided into following experiment method：
1, four unit experiment rooms do not add chemical agent, the temperature, gas concentration lwevel in control group and original training
Foster amount is consistent.First experimental group, the second experimental group are identical with the temperature value in third experimental group, and gas concentration lwevel
From high to low, the gas concentration lwevel of the first experimental group is higher than the gas concentration lwevel in control group, and two in third experimental group
Concentration of carbon is aoxidized less than the gas concentration lwevel in control group, and the maximum concentration of carbon dioxide and the difference of minimum concentration are
0.6%, after cultivating the same time, carbon dioxide identical in temperature condition is drawn by above experimental result
Influence of the concentration to growth of tumour cell；
2, four unit experiment rooms do not add chemical agent, the temperature, gas concentration lwevel in control group and original training
Foster amount is consistent.First experimental group, the second experimental group are identical with the carbon dioxide concentration value in third experimental group, and temperature
From high to low, the temperature of the first experimental group is higher than the temperature in control group, and the temperature in third experimental group is less than in control group
Temperature, and the difference of temperature maximum and minimum value is 10 DEG C, and after cultivating the same time, temperature is obtained by above experimental result
In the case of degree condition is identical, influence of the temperature to growth of tumour cell；
3, control group, the first experimental group, the second experimental group and third experimental group temperature, gas concentration lwevel and original culture
Amount be consistent, chemical agent is not added in control group, is noted respectively in the first experimental group, the second experimental group and third experimental group
The different chemical agents for tumour cell is penetrated, injection volume is identical, and growing state is observed after cultivating the same time.
The growing environment and drug effect pair of tumour cell can be judged by way of above three groups of control variable
The influence of the growth of tumour cell.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of device for growth of tumour cell Inhibition test, including babinet (1), it is characterised in that：The babinet (1)
Uniformly longitudinal grafting is there are three partition board (2) for inner cavity, and the partition board (2) is fixedly connected with the inner cavity of babinet (1) by screw, three
Babinet (1) is divided into four unit experiment rooms by the partition board (2), is hinged on front side of the unit experiment room and is picked and placeed door (3),
Straight slot laterally is offered in the middle part of the antetheca for picking and placeing door (3), and straight slot has been internally embedded viewing window (4), the unit
The top in laboratory is embedded with head cover (5), and rubber stopper (6), the left side of the head cover (5) are embedded in the middle part of the head cover (5)
Longitudinal direction is plugged with air inlet pipe (7), and flow control valve (8), the flow control valve are equipped on the pipeline of the air inlet pipe (7)
(8) be located at the upside of head cover (5), the right side of the head cover (5) is longitudinally plugged with escape pipe (9), the rubber stopper (6) be located at into
Between tracheae (7), escape pipe (9), the bottom of the unit experiment room is connected with current regulator (10), the unit experiment room
Inner wall incubation cavity (12) is bonded with by bonding agent, the inner wall of the incubation cavity (12) is connected with electrical heating by buckling circumferential direction
It manages (11), the electric heating tube (11) is electrically connected with current regulator (10), in the middle part of the intracavity bottom of the incubation cavity (12)
It is connected by screw column (13), the top of the column (13) is welded with material containing disk, and the top braces of material containing disk have reality
Test vessel (14).
2. a kind of device for growth of tumour cell Inhibition test according to claim 1, it is characterised in that：The sight
The height for examining windowpane (4) is identical as the height of experimental ware (14).
3. a kind of device for growth of tumour cell Inhibition test according to claim 1, it is characterised in that：The top
The dimensional fits mode covered between (5) and rubber stopper (6) is interference fit.
4. a kind of device for growth of tumour cell Inhibition test according to claim 1, it is characterised in that：It is described into
The bottom end of tracheae (7) stretches in the middle part of the inner cavity of experimental ware (14).
5. a kind of device for growth of tumour cell Inhibition test according to claim 1, it is characterised in that：It is described go out
The bottom end of tracheae (9) is connected with flaring gas gathering mask.
6. a kind of device for growth of tumour cell Inhibition test according to claim 1, it is characterised in that：It is described go out
It is embedded with dustproof sponge plug on the upside of the inner cavity of tracheae (9).
7. a kind of device for growth of tumour cell Inhibition test according to claim 1, it is characterised in that：The electricity
It is sliding rheostat current regulator to flow modulator (10).
8. a kind of device for growth of tumour cell Inhibition test according to claim 1, it is characterised in that：The guarantor
Warm chamber (12) is calcium silicate heat-preserving chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810571505.1A CN108715808A (en) | 2018-06-06 | 2018-06-06 | A kind of device for growth of tumour cell Inhibition test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810571505.1A CN108715808A (en) | 2018-06-06 | 2018-06-06 | A kind of device for growth of tumour cell Inhibition test |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108715808A true CN108715808A (en) | 2018-10-30 |
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ID=63911792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810571505.1A Pending CN108715808A (en) | 2018-06-06 | 2018-06-06 | A kind of device for growth of tumour cell Inhibition test |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114401380A (en) * | 2022-01-25 | 2022-04-26 | 中国人民解放军总医院第八医学中心 | Cell compatibility testing system and method for preparing bionic bone based on composite material |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020009803A1 (en) * | 1997-01-08 | 2002-01-24 | Gabor Vajta | Method and apparatus for culturing cells and tissues |
| CN205529172U (en) * | 2016-01-28 | 2016-08-31 | 黑龙江大学 | Spinning equipment is drawn with carrying to experiment teaching |
| CN207079238U (en) * | 2017-06-15 | 2018-03-09 | 天津冠勤医药科技有限公司 | A kind of device for growth of tumour cell Inhibition test |
-
2018
- 2018-06-06 CN CN201810571505.1A patent/CN108715808A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020009803A1 (en) * | 1997-01-08 | 2002-01-24 | Gabor Vajta | Method and apparatus for culturing cells and tissues |
| CN205529172U (en) * | 2016-01-28 | 2016-08-31 | 黑龙江大学 | Spinning equipment is drawn with carrying to experiment teaching |
| CN207079238U (en) * | 2017-06-15 | 2018-03-09 | 天津冠勤医药科技有限公司 | A kind of device for growth of tumour cell Inhibition test |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114401380A (en) * | 2022-01-25 | 2022-04-26 | 中国人民解放军总医院第八医学中心 | Cell compatibility testing system and method for preparing bionic bone based on composite material |
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Application publication date: 20181030 |
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