CN1087148C - Sea cucumber enzymolyzing liquid products series and their production process - Google Patents
Sea cucumber enzymolyzing liquid products series and their production process Download PDFInfo
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- CN1087148C CN1087148C CN99113247A CN99113247A CN1087148C CN 1087148 C CN1087148 C CN 1087148C CN 99113247 A CN99113247 A CN 99113247A CN 99113247 A CN99113247 A CN 99113247A CN 1087148 C CN1087148 C CN 1087148C
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Abstract
The present invention relates to a serial product of sea cucumber enzymolysis liquid and a processing method thereof. The serial product of sea cucumber enzymolysis liquid is characterized in that the enzymolysis liquid comprises the components according to the weight percentage of 5 to 20% of active peptide classes of peptide 2 to 10, 10 to 55% of amino acids, 0.1 to 1.5% of holothurin, 0.5 to 5% of mucopolysaccharide and water as the rest. The preparation method of the enzymolysis liquid adopts double-enzymatic hydrolysis or single-enzymatic hydrolysis, and hydrolysis degrees are from 50 to 75%, wherein 5 to 20% of the components can be hydrolyzed into the active peptide of the peptide 2 to 10. The enzymolysis liquid is used as basic liquid, 0 to 60% of additives are added so as to prepare sea cucumber nutrition serial products, so that the serial product of sea cucumber enzymolysis liquid has the efficiency of easy digestion and absorption, aging resistance, blood pressure reduction, etc.
Description
The present invention relates to a kind of edible protein worked structure processing, especially sea cucumber hydrolysate series of products and processing method thereof.It is to be raw material with natural sea cucumber, by biological enzymolysis technology, makes enzymolysis liquid, further is processed into sea cucumber nutrient agent, satisfies people's the reinforcement body curative effect and the needs of body-building.
As everyone knows, sea cucumber contains rich in protein, amino acid and various trace elements, is regarded as efficient invigorant, up to the present, people to sea cucumber and processing after eating method have following several:
1, culinary art: its shortcoming is to have lost a large amount of natural effective ingredients in the processing;
2, eat raw: edible after cold working, fishy smell is too dense, nondigestible and absorption;
3, the sea cucumber nutrient agent of scientific method processing is oral liquid, pulvis, tablet etc., is the manufacture method ＂ of a ＂ sea cucumber milk extract of 88105228 records as the disclosed application number of State Patent Office; Application number is the manufacture method ＂ of 90108261.9 disclosed ＂ Haicibao liquids; Application number is that the ＂ full nature health oral administration liquid ＂ and the application number of 93115913.X report is among 98113896.9 disclosed ＂ full holothurian capsule and the manufacture method ＂ thereof, above document discloses fresh sea cucumber or has sent out system dried sea-cucumber processing brewing method and preparation thereof, its shortcoming is that enzymolysis process can not become the sea cucumber enzymolysis bioactive peptide enzymolysis liquid of 2-10 peptide, brings into play trophism and assimilation effect better thereby influence it.
The invention provides the zymolysis technique of a kind of natural sea cucumber, the sea cucumber hydrolysate series of products that make contain the bioactive peptide of a large amount of 2-10 peptides, have the advantage of easily digesting and assimilating, but also have special effect, are longevity factors as glutathione, can be anti-ageing; Blood pressure lowering peptide has antihypertensive function, but normal person's blood pressure is not had hypotensive effect, is base fluid with the enzymolysis liquid with these features, can further be processed into series of products such as holothurian oral liquid, particle or tablet.
Another object of the present invention is to provide a kind of above-mentioned sea cucumber hydrolysate series of products and processing method thereof produced.Method.
Above-mentioned purpose of the present invention realizes by following technological means.
A kind of sea cucumber hydrolysate is the base fluid of sea cucumber nutrient preparation, it is characterized in that the composition of this enzymolysis liquid, weight percent content are as follows:
The bioactive peptide 5%～20% of 2-10 peptide;
Amino acid and the low nitrogenous thing of molecule: 10%～55%;
Holothurin: 0.1%～1.5%;
Mucopolysaccharide: 0.5%～5%;
Surplus: water.
Contain known compositions such as various trace elements, carbohydrate, vitamin, fat in this enzymolysis liquid.
The processing technology of this enzymolysis liquid is as follows: the sea cucumber dry product steeps to be sent out, and vertically cuts open along a side jaggy with cutter then, removes impurity in ginseng mouth and the chamber, cleans in clear water; And fresh sea cucumber inserts from the sea cucumber anus with scissors, cuts open along belly, and cutting should try one's best when cuing open intactly takes out extra large intestines, and residue in the intestines is extruded, and with clear water sea cucumber body wall and sea cucumber intestine silt is cleaned.To clean instrument before the operation, avoid greasy dirt.Then sea cucumber and the sea cucumber intestine of cleaning broken into slurries repeatedly with stainless steel meat grinder or beater, use colloid mill further levigate again, make and contain sea cucumber 4%～10% sea cucumber milk, be beneficial to the carrying out of enzyme hydrolysis process the sea cucumber slurries.Enzyme hydrolysis is the key that makes high nutrition hydrolyzate.The present invention has adopted dual-enzyme hydrolysis method or single enzymolysis method, and these two kinds of methods all have percent hydrolysis height, effective, mild condition, protein digestion become characteristics such as the bioactive peptide of 2-10 peptide and amino acid effectively.The processing method of this enzymolysis liquid comprises fresh sea cucumber or sends out the dried sea-cucumber make, through washing, fragmentation, enzyme hydrolysis, the enzyme that goes out, filter, take off raw meat processing processing, the enzyme hydrolysis process employing dual-enzyme hydrolysis method or the single enzymolysis method of this method.
Dual-enzyme hydrolysis method: the first step is to add bacillus subtilis neutral proteinase in the sea cucumber slurries, making enzyme concentration is 3～6U/ml, at PH is 6～8.5, temperature is under 40～55 conditions, hydrolysis 1.5 hours～4 hours, second step was to add pepsin in the solution after first step enzyme hydrolysis, make that pepsic concentration is 4.5～9U/ml in the solution, at PH is 2.5～4.5, temperature is further hydrolysis under 40～55 conditions, hydrolysis time is 0.5～4 hour, and second step also can be selected the adding bromelain for use, makes in the solution after the first step enzyme hydrolysis, the bromelain enzyme concentration is 2 ‰, at PH is 6-8, and temperature is under 40,0～50 condition, enzymolysis 1 hour～4 hours;
The single enzymolysis method: adding bromelain in the sea cucumber slurries, make that enzyme concentration is 2 ‰ in this solution, is 6～8 at PH, and temperature is under 30～50 conditions, enzymolysis 2 hours～6 hours.
In above-mentioned two kinds of enzyme hydrolysis process, the adjustment of pH value realizes by adding hydrochloric acid or potassium hydroxide, NaOH in solution; Also can add neutral buffered liquid as potassium hydrogen phosphate, dibastic sodium phosphate class and guarantee the stable of pH value in the enzymolysis process, this method also has requirement to the performance of enzyme, reach 105 units as the bacillus subtilis neutral proteinase vigor, prolease activity reaches 3000 units, and the bromelain enzyme activity should reach 500,000 units.These two kinds of methods can guarantee that the sea cucumber degree of hydrolysis all can reach 50%～75%, wherein 5%～20% hydrolyzable is the bioactive peptide of 2-10 peptide, and this nutrition is is not only easily digested and assimilated, but also has some specific functions, as glutathione is longevity factor, the anti-ageing factor; Blood pressure lowering peptide has antihypertensive function, but normal person's blood pressure is not had hypotensive effect, is base fluid with the enzymolysis liquid with these features, can further be processed into series of products such as oral liquid, powdery, particle or tablet.
Enzymolysis liquid behind the enzymolysis reaches to give after measured decides component content, and the enzyme that must in time go out is about to enzymolysis liquid and is heated to 100, keeps for 30 seconds, and enzymolysis stops, and can filter, and removes impurity, obtains qualified enzymolysis liquid.Make this enzymolysis liquid as the base fluid of making sea cucumber nutrient agent, also can in enzymolysis liquid, add the food flavor enzyme of 1～3U/ml, remove the bitter taste that some small peptides produce.This method also comprises takes off raw meat and the processing that eliminates the unusual smell; concrete process is as follows: add 0.5%～3% (weight ratio) β-CD and boiled 2-10 minute in enzymolysis liquid; or in enzymolysis liquid, add 0.5%～0.8% (weight ratio) dusty yeast; 40 are incubated 10 minutes～35 minutes down; can make the bioactive peptide that contains a large amount of 2-10 peptides; amino acid; holothurin; the enzymolysis liquid of mucopolysaccharide and various trace elements; it can directly make oral liquid or powder or tablet; can this enzymolysis liquid be base fluid also; the additive with 0%-60% (weight ratio) of joining is made sea cucumber nutrient preparation of the same type.The present invention improves the health, strengthens body, anti-ageing and replenish the calcium easy absorption, the good sea cucumber nutrient preparation of effect are provided for the mankind.
Below in conjunction with embodiment the present invention is further elaborated:
Embodiment 1, dual-enzyme hydrolysis method: fresh sea cucumber is inserted from the nearly anus of sea cucumber belly with microscler pocket knife, cut open along belly, with the complete taking-up of sea cucumber intestine, removing the interior residue of intestines extrudes, after with clear water sea cucumber body wall and sea cucumber intestine silt being cleaned, sea cucumber and the sea cucumber intestine of cleaning broken into slurries repeatedly with beater, use colloid mill further levigate again the sea cucumber slurries, make the sea cucumber milk that contains sea cucumber 4.5%, add the bacillus subtilis neutral proteinase of enzyme activity 105 units, the concentration that makes hydrolyzate is 5U/ml, and its PH is 7, temperature is 45, after hydrolysis time was 3 hours, it was the pepsin of 3000 units that second step was selected enzyme activity, and the concentration that makes hydrolyzate is the aqueous solution of 8U/ml, its PH is 3, temperature is 45, further hydrolysis, and enzymolysis time is 1.5 hours.
Embodiment 2, the single enzymolysis method: the sea cucumber dry product steeps to be sent out, and vertically cuts open along a side jaggy with cutter then, removes impurity in ginseng mouth and the chamber, cleans in clear water.Then the sea cucumber of cleaning is broken into slurries repeatedly with beater, use colloid mill further levigate again the sea cucumber slurries, make the sea cucumber milk that contains sea cucumber 4.5%, adding enzyme activity is the bromelain of 500,000 units, the concentration that makes hydrolyzate is 2 ‰, adds the cushioning liquid of KH2PO4-NaOH again, and making sea cucumber hydrolyzate PH is 7, in temperature is under the 45 conditions, enzymolysis 4.5 hours;
Embodiment 3, and holothurian oral liquid composition and percentage by weight are as follows: base fluid: honey: fruit juice: potassium sorbate: citric acid: sterilized water is 100: 10: 10: 0.1: 0.08: 50, and through the sterilization bottling.
Embodiment 4, and sea cucumber microcapsules powder composition and percentage by weight are as follows: base fluid: lactose: the methyl fiber: water is 100: 90: 10: 1000, mix the back spray-drying, and make sea cucumber hydrolysate by the powder of microencapsulation, the dress capsule gets final product.
Embodiment 5, the sea cucumber tablet, composition and percentage by weight are as follows: the spray-dried one-tenth powder of base fluid 1000 (weight ratio), adding concentration are 10% starch slurry 10 (weight ratio). mixes and makes wet granular, dry under 70, cross 16 mesh sieves, make the granule of water content 5%, add 5% talcum powder, stir evenly, compressing tablet, sugar coating again, polishing.
Claims (7)
1, a kind of preparation method of sea cucumber hydrolysate, it comprises fresh sea cucumber or sends out the dried sea-cucumber make, through washing, fragmentation, enzyme hydrolysis, the enzyme that goes out, filter, take off raw meat processing processing, the enzyme hydrolysis employing dual-enzyme hydrolysis method or the single enzymolysis method of this method;
Dual-enzyme hydrolysis method: the first step is to add bacillus subtilis neutral proteinase in the sea cucumber slurries, making enzyme concentration is 3～6U/ml, at PH is 6～8.5, temperature is under 40 ℃～55 ℃ conditions, hydrolysis 1.5 hours～4 hours, second step was to add pepsin in the solution after first step enzyme hydrolysis, make that pepsic concentration is 4.5～9U/ml in the solution, at PH is 2.5～4.5, temperature is further hydrolysis under 40 ℃～55 ℃ conditions, hydrolysis time is 0.5～4 hour, perhaps second step was to add bromelain in the solution after first step enzyme hydrolysis, and making the bromelain enzyme concentration is 2 ‰, is 6-8 at PH, temperature is under 40 ℃～50 ℃ conditions, enzymolysis 1 hour～4 hours;
The single enzymolysis method: adding bromelain in the sea cucumber slurries, make that enzyme concentration is 2 ‰ in this solution, is 6～8 at PH, and temperature is under 30 ℃～50 ℃ conditions, enzymolysis 2 hours～6 hours.
2, a kind of sea cucumber hydrolysate by the preparation of the described method of claim 1 is characterized in that the component of this enzymolysis liquid, weight percent content are as follows:
The active peptide of 2-10 peptide: 5%～20%;
Amino acid and the low nitrogenous thing of molecule: 10%～55%;
Holothurin: 0.1%～1.5%;
Mucopolysaccharide: 0.5%～5%;
Surplus: water.
3, a kind of product that contains sea cucumber hydrolysate is characterized in that sea cucumber hydrolysate as claimed in claim 2 (percentage by weight) is 100%, is equipped with 60% additive again, is processed into oral liquid, powdery, particle or tablet.
4, a kind of holothurian oral liquid is characterized in that being made by following method: sea cucumber hydrolysate as claimed in claim 2: honey: fruit juice: potassium sorbate: citric acid: sterilized water is 100: 10: 10: 0.1: 0.08: 50 (weight ratio), and through the sterilization bottling.
5, a kind of sea cucumber microcapsules powder, it is characterized in that being made by following method: sea cucumber hydrolysate as claimed in claim 2: lactose: the methyl fiber: water is 100: 90: 10: 1000 (weight ratios), mix the back spray-drying, make the powder of microencapsulation.
6, the preparation method of enzymolysis liquid according to claim 1 is characterized in that the said enzyme that goes out is that enzymolysis liquid is heated to 100 ℃, keeps for 30 seconds.
7, the preparation method of enzymolysis liquid according to claim 1 is characterized in that taking off raw meat processing and is treated to and adds 0.5%～3% (weight ratio) β-CD boiled 2-10 minute in enzymolysis liquid; Perhaps add 0.5%～0.8% (weight ratio) dusty yeast in enzymolysis liquid, 40 ℃ are incubated 25 minutes～35 minutes down.
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| CN99113247A CN1087148C (en) | 1999-09-03 | 1999-09-03 | Sea cucumber enzymolyzing liquid products series and their production process |
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| CN99113247A CN1087148C (en) | 1999-09-03 | 1999-09-03 | Sea cucumber enzymolyzing liquid products series and their production process |
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| CN1087148C true CN1087148C (en) | 2002-07-10 |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6916492B2 (en) | 2001-03-30 | 2005-07-12 | Council Of Scientific & Industrial Research | Natural nontoxic multicolor fluorescent protein dye from a marine invertebrate, compositions containing the said dye and its uses |
| US6689391B2 (en) | 2001-03-30 | 2004-02-10 | Council Of Scientific & Industrial Research | Natural non-polar fluorescent dye from a non-bioluminescent marine invertebrate, compositions containing the said dye and its uses |
| CN101015361B (en) * | 2007-02-10 | 2010-09-01 | 中国海洋大学 | Instant sea cucumber dissolved granule and its preparing process |
| CN102242050B (en) * | 2011-07-07 | 2014-08-27 | 河北农业大学 | Preparation method of sea cucumber vinegar |
| CN102580063B (en) * | 2012-03-08 | 2013-09-04 | 宁波大学 | Preparation method of xanthine oxidase inhibitor |
| CN104473156A (en) * | 2014-11-25 | 2015-04-01 | 荣成宏业海洋科技有限公司 | Processing method of sea cucumber oral liquid |
| CN104719970A (en) * | 2015-02-27 | 2015-06-24 | 蓬莱市海洋生物有限公司 | Production process of sea cucumber oral liquid |
| CN113201562A (en) * | 2015-03-26 | 2021-08-03 | 浙江泰利森药业有限公司 | Process for enzymolysis of sea cucumber into multiple amino acids |
| CN108576371A (en) * | 2018-03-30 | 2018-09-28 | 大连工业大学 | A kind of microcapsule embedded double protein peptide and preparation method thereof |
| CN110279047A (en) * | 2019-08-05 | 2019-09-27 | 山东省科学院生物研究所 | A kind of sea cucumber fruit-flavored beverage and its processing method |
| CN111418840A (en) * | 2020-05-12 | 2020-07-17 | 烟台五神生物科技有限公司 | Health food for preventing and treating sarcopenia |
| CN113584109A (en) * | 2021-08-03 | 2021-11-02 | 青岛纽萃生生物健康科技有限公司 | Preparation method of holothurian polypeptide with high polysaccharide and low fishy smell based on whole-course low-heat-temperature neutralization technology |
| CN114568610A (en) * | 2021-12-31 | 2022-06-03 | 山东点尤尤生物科技有限公司 | Sea cucumber sugar-free anti-fatigue beverage and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1146709A (en) * | 1994-02-28 | 1997-04-02 | 拜奥蔡姆系统公司 | Euphausiid harvesting and processing method and apparatus |
| CN1163723A (en) * | 1996-04-30 | 1997-11-05 | 中国科学院大连化学物理研究所 | Enzyme hydrolysis method for shellfish |
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1999
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1146709A (en) * | 1994-02-28 | 1997-04-02 | 拜奥蔡姆系统公司 | Euphausiid harvesting and processing method and apparatus |
| CN1163723A (en) * | 1996-04-30 | 1997-11-05 | 中国科学院大连化学物理研究所 | Enzyme hydrolysis method for shellfish |
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