CN108703973A - A kind of external-use gel preparation method for treating periodontitis - Google Patents
A kind of external-use gel preparation method for treating periodontitis Download PDFInfo
- Publication number
- CN108703973A CN108703973A CN201710229630.XA CN201710229630A CN108703973A CN 108703973 A CN108703973 A CN 108703973A CN 201710229630 A CN201710229630 A CN 201710229630A CN 108703973 A CN108703973 A CN 108703973A
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- Prior art keywords
- periodontitis
- gel
- temperature plasma
- plasma
- gel according
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/02—Medicinal preparations containing materials or reaction products thereof with undetermined constitution from inanimate materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61K47/38—Cellulose; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
- A61K9/0063—Periodont
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
Abstract
The present invention provides a kind of for treating gel of periodontitis and preparation method thereof.It is provided by the present invention to be for treating gel of periodontitis and preparation method thereof：1) atmos low-temperature plasma is passed through in sterile deionized water, acts on 10-40min, obtain the plasma-activated liquid rich in living radical；2) hypromellose is added into the activating solution, a concentration of 0.5%-1.5% stirs and evenly mixs abundant reaction, is statically placed in 4 DEG C.The gel being prepared by the method for the invention enters oral pocket using syringe injection；By the gel for the treatment periodontitis that this method makes, oral pocket pathogenic infection can be effectively reduced, alleviates the state of an illness, has no toxic side effect, production method is simple, economical, can preserve use for a long time.
Description
Technical field
The present invention relates to the external-use gel preparations and preparation method thereof that a kind of department of stomatology is used to treat periodontitis.
Background technology
Periodontitis is that a kind of common disease of the mankind and high morbidity, the World Health Organization assert that it is cancer, angiocardiopathy
Afterwards, the third-largest killer of the mankind is threatened.Including disease caused by gum, parodontium, alveolar bone chronic infection.It can cause such as gum
Inflammation, bleeding, form oral pocket, and knife absorption of alveolar bone, tooth mobility displacement can lead to loss of tooth when serious.In addition, tooth
All situations and whole body health are also closely connected, and the pathogenic bacteria being present in periodontium can generate endotoxin, may
Cause the other diseases such as atherosclerosis, myocardial infarction.
Drug used at present is mainly based on antibiotic, and Formulations for systemic administration such as first nitre file etc., side effect is larger, is easy to cause
Flora imbalance generates drug resistance, and cost is higher；Local application's such as minocycline, for nitre file etc., local application is currently
The prefered method for the treatment of periodontitis, but local application's higher price.
Plasma is the 4th state of substance, and low temperature plasma is due to temperature near room temperature, freely containing abundant activity
Base, such as hydroxyl, superoxide anion and active nitrogen have significant bactericidal and bacteriostatic effect, and there is wide biomedicine to answer
With.Atmos low-temperature plasma activated water equally contains these living radicals, can effectively bacteria removal and its biomembrane,
Have effects that antibacterial anti-inflammatory, and does not allow to be also easy to produce drug resistance.
Invention content
The present invention prepares the gel for the treatment of periodontitis using atmos low-temperature plasma technology.Clinically mainly adopt at present
Treat periodontitis with the mode of antibiotic part medication, but cost is higher, easy tos produce drug resistance, atmos low-temperature etc. from
Contain a large amount of living radical in daughter activated water, for the pathogenic bacteria that periodontitis is common, such as porphyromonas gingivalis is glued and put
Line bacterium etc. has significant lethal effect.It is good, at low cost that the object of the present invention is to provide a kind of therapeutic effects, has no toxic side effect
Gel preparation and preparation method thereof for treating periodontitis.
It is provided by the present invention for treat the gel of periodontitis to be to be prepared as steps described below：
(1) atmos low-temperature plasma is passed through in sterile deionized water, opens air source to atmos low-temperature plasma
It is passed through working gas, regulation power and gas flow rate in body generator, high voltage power supply is opened after steady air current, processing obtains
Plasma-activated liquid rich in living radical；
(2) by the above-mentioned activation pension gelation rich in hydroxy radical to get to the gel preparation for treating periodontitis.
In above method step (1), the atmos low-temperature plasma passes through atmos low-temperature plasma generator
It generates.
Generate atmos low-temperature plasma operation be：It is logical to atmos low-temperature plasma generator with certain flow rate
Enter working gas, start high voltage power supply after steady air current, excites atmos low-temperature plasma generator, make atmos low-temperature
Plasma and deionized water contact action certain time, obtain the plasma-activated liquid rich in active oxygen radical.
The working gas can be selected from the mixed gas of one or more of following gases：Argon gas, helium, sky
Gas and oxygen.
The processing power of atmos low-temperature plasma processing is 10-100W, concretely 50W, processing time be
5min-30min, concretely 10min.
The flow velocity of the working gas is 2-5L/min, concretely 3L/min.
The time of the effect is 10-40min.
In above method step 2), the concrete operations for making the plasma-activated pension gelation rich in living radical
For：Gelling agent hypromellose (HPMC) is added into the plasma-activated liquid rich in hydroxy radical, stirring is equal
It is even, so that it is fully reacted.
In order to increase stability, a concentration of 0.5%-1.5% of hypromellose
In above method step 2), the stirring carries out at normal temperatures, and the time of the stirring is 1min-5min, makes it
Fully reaction.
The gelling performance that the present invention is prepared using hypromellose (or chitosan or carbomer) is stablized, can be into
Row is biodegradable, and gel skeleton is formed after water-soluble liquid is encountered in periodontium.In gel containing a large amount of free radical at
Point, slow release can be carried out in oral pocket, achieve the purpose that treat periodontitis, and the raw material used are mainly deionized water
And gelling agent, cost are relatively low.
Description of the drawings
Fig. 1 prepares schematic diagram for periodontal gel of the invention.1 is low-temperature plasma generator；2 atmos low-temperatures etc.
Gas ions flame；3 deionized waters.
Fig. 2 is periodontal gel bacteriostasis.
Specific implementation mode
Below by specific embodiment, the present invention will be described, but does not constitute any limitation of the invention.
Experimental method used in following embodiments is conventional method unless otherwise specified；Institute in following embodiments
Reagent, material etc., are commercially available unless otherwise specified.
Embodiment 1
(1) atmos low-temperature plasma generator is opened, argon gas/oxygen (containing 2% oxygen) is carried out with the power of 10W
Atmos low-temperature plasmaization processing；
(2) 50mL sterile deionized waters are taken, the low temperature plasma after excitation is passed through water middle-range with the flow velocity of 2L/min
1cm under chaotropic face, action time 10min obtain the plasma-activated liquid rich in living radical；
(3) hypromellose is added in plasma-activated liquid, adjust its a concentration of 0.5%, stir at normal temperatures
1min is mixed, the gel that 10min can be obtained required preparation is stood.
Embodiment 2
(1) atmos low-temperature plasma generator is opened, atmos low-temperature plasma is carried out to air with the power of 50W
Bodyization processing；
(2) 50mL sterile deionized waters are taken, the low temperature plasma after excitation is passed through water middle-range with the flow velocity of 3L/min
1cm under chaotropic face, action time 25min obtain the plasma-activated liquid rich in living radical；
(3) hypromellose is added in plasma-activated liquid, adjust its a concentration of 1%, stir 3 at normal temperatures
Minute, stand the gel that 10min can be obtained required preparation.
Embodiment 3
(1) atmos low-temperature plasma generator is opened, argon gas/nitrogen mixed gas is carried out with the power of 100W big
The processing of air pressure low temperature plasmaization；
(2) 50mL sterile deionized waters are taken, the low temperature plasma after excitation is passed through water middle-range with the flow velocity of 5L/min
1cm under chaotropic face obtains the plasma-activated liquid rich in living radical after acting on 5min；
(3) hypromellose is added in plasma-activated liquid, adjust its a concentration of 1.5%, stir at normal temperatures
It mixes 5 minutes, stands the gel that 10min can be obtained required preparation.
Comparative example 1
Negative control group (Blank gel)：The plasma tooth prepared by deionized water 50mL, 1% hypromellose
All gels；The gel that experimental group is prepared using plasma-activated liquid；It normally does without any processing.
6 cleaning grade Golden Hamsters be randomly divided into normal group, periodontitis group, experimental group.Normal group full diet, does not appoint
It is pre- that connection, periodontitis group, the experimental group neck portion of filament constriction experiment tooth, feeding high-carbonhydrate diet, Oral inoculation pathogenic bacteria tooth
Gum Detection of Porphyromonas, actinomyces viscosus are solidifying using plasma periodontal on the basis of embodiment 2 to form experimental animal model of periodontitis
Glue treats Hamsters Experimental Periodontitis, is receiving to treat 12h, and for 24 hours and after 48h, periodontal is calculated using sterilizing paper point method
Bacterial infections levels in bag, for evaluating curative effect.
The result shows that compared with control group (CONTROL), bacterium can be effectively reduced using plasma periodontal gel
Level (P<0.05), alleviate inflammation, to achieve the purpose that treat periodontitis.
Claims (8)
1. a kind of gel preparation for treating periodontitis, it is characterised in that：Gel main component is atmos low-temperature plasma
The two is mixed to prepare gel preparation by body activating solution and gelling agent.
2. the gel according to claim 1 for periodontitis, it is characterised in that：Plasma-activated liquid is by following steps
It prepares, working gas is passed through to atmos low-temperature plasma generator with certain flow rate, starts high-voltage electricity after steady air current
Source excites atmos low-temperature plasma generator, makes atmos low-temperature plasma and one timing of deionized water contact action
Between, obtain the plasma-activated liquid rich in active oxygen radical.
3. the gel according to claim 1 for periodontitis, it is characterised in that：The gelling agent is that hydroxypropyl is fine
Dimension element, chitosan and carbomer one of which or two or more mixtures.
4. the gel according to claim 1 for periodontitis, it is characterised in that：Make the activating solution rich in living radical
Mixed method with gelling agent is：Hypromellose, mixing time is added into the activating solution rich in hydroxy radical
For 1min-5min.
5. the gel according to claim 2 for periodontitis, it is characterised in that：The atmos low-temperature plasma with
The time of deionized water effect is 10-40min.
6. the gel according to claim 2 for periodontitis, it is characterised in that：The working gas be selected from it is following at least
One or more kinds of mixed gas are mixed with arbitrary proportion：Air, oxygen, argon gas and nitrogen.
7. the gel according to claim 2 for periodontitis, it is characterised in that：The high voltage power supply power is 10-
100W。
8. the gel according to claim 3 for periodontitis, it is characterised in that：Hypromellose it is a concentration of
0.5%-1.5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710229630.XA CN108703973A (en) | 2017-04-10 | 2017-04-10 | A kind of external-use gel preparation method for treating periodontitis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710229630.XA CN108703973A (en) | 2017-04-10 | 2017-04-10 | A kind of external-use gel preparation method for treating periodontitis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108703973A true CN108703973A (en) | 2018-10-26 |
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ID=63866143
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710229630.XA Pending CN108703973A (en) | 2017-04-10 | 2017-04-10 | A kind of external-use gel preparation method for treating periodontitis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109890121A (en) * | 2019-04-01 | 2019-06-14 | 西安交通大学 | A kind of plasma producing apparatus and plasma gel process for preparing |
| CN111434707A (en) * | 2019-01-11 | 2020-07-21 | 北京大学 | Antibacterial hydrogel |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100209293A1 (en) * | 2007-09-27 | 2010-08-19 | Satoshi Ikawa | Sterilization method and apparatus |
| CN106420373A (en) * | 2015-08-06 | 2017-02-22 | 北京大学 | Gel for tooth whitening and preparation method thereof |
-
2017
- 2017-04-10 CN CN201710229630.XA patent/CN108703973A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100209293A1 (en) * | 2007-09-27 | 2010-08-19 | Satoshi Ikawa | Sterilization method and apparatus |
| CN106420373A (en) * | 2015-08-06 | 2017-02-22 | 北京大学 | Gel for tooth whitening and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 刘得玺: "大气压非平衡等离子体对牙龈卟啉单胞菌的作用及其生物安全性评价", 《万方数据（HTTP://WWW.WANFANGDATA.COM.CN/DETAILS/DETAIL.DO?_TYPE=DEGREE&ID=D190215）》 * |
| 国家食品药品监督管理总局执业药师资格认证中心组织编写: "《药学专业知识.1》", 31 January 2016, 中国医药科技出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111434707A (en) * | 2019-01-11 | 2020-07-21 | 北京大学 | Antibacterial hydrogel |
| CN109890121A (en) * | 2019-04-01 | 2019-06-14 | 西安交通大学 | A kind of plasma producing apparatus and plasma gel process for preparing |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication | ||
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Application publication date: 20181026 |