CN108690114A - A kind of preparation method of seed of feather cockscomb glycosides A - Google Patents
A kind of preparation method of seed of feather cockscomb glycosides A Download PDFInfo
- Publication number
- CN108690114A CN108690114A CN201710227702.7A CN201710227702A CN108690114A CN 108690114 A CN108690114 A CN 108690114A CN 201710227702 A CN201710227702 A CN 201710227702A CN 108690114 A CN108690114 A CN 108690114A
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- China
- Prior art keywords
- seed
- feather cockscomb
- glycosides
- mesh
- preparation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to a kind of pharmaceutical technology fields, and in particular to a kind of preparation method of seed of feather cockscomb glycosides A, processing step include:(1)Seed of feather cockscomb medicinal material is cleaned up, the segment of 2-3cm is cut into, spare, 80 mesh crush;(2)Continuous flow upstream ultrasonic extraction;(3)Polyamide purifies, ethanol elution, collects eluent;(4)It is concentrated and dried to get seed of feather cockscomb glycosides A.The present invention is simple for process, extraction time is short, recovery rate is high using continuous flow upstream ultrasonic extraction-polyamide purification seed of feather cockscomb glycosides A, and production cost is low, can carry out industrialized production.
Description
Technical field:
The invention belongs to active ingredient of natural product separation fields, and in particular to a kind of to utilize continuous flow upstream ultrasonic extraction seed of feather cockscomb
The method of glycosides A.
Background technology:
The seed of feather cockscomb is the plant that Amaranthaceae feather cockscomb belongs to, be the celosia argentea L.seed after maturation is dried it is obtained, according to middle peasant's book on Chinese herbal medicine
The record of warp, the drug have effects that clear liver and improve vision, and cure mainly eye-blurred, hepatopyretic vertigo, screen of looking unfamiliar film, the hot hot eyes of liver etc.
Symptom.Hepatic injury is a kind of pathological state by caused by a variety of diseases, such disease incidence is higher, and is difficult to cure,
The case where easily causing recurrent exerbation, and develop as serious diseases such as hepatic sclerosis, fibrosis and liver cancer, and Chinese medicine thinks green
Feather cockscomb has extremely strong protective effect for liver.Therefore the extraction process of research seed of feather cockscomb glycosides A deeply develops the seed of feather cockscomb
Using having great importance.
Celosin A is white powder, soluble easily in water, molecular formula C41H62O15, currently, the extraction about seed of feather cockscomb glycosides A
Report it is less, and mostly use traditional extraction method.And traditional extraction method exists since temperature is excessively high can destroy compound
Structure influences the problems such as its bioactivity and yield reduce, and is not suitable for industrialized production.And the continuous flow upstream ultrasonic extraction seed of feather cockscomb
Plant tissue relatively mild can both be crushed, decomposed, and improved yield by a relatively large margin by glycosides A, while be also less prone to destroy the vertical of ingredient
Body structure.
Invention content:
In view of the deficiencies of the prior art, it is an object of the present invention to provide the sides of short, high income the seed of feather cockscomb glycosides A of extraction time a kind of
Method.
The present invention includes the following steps:
Seed of feather cockscomb medicinal material is cleaned up, the segment of 2-3cm is cut into, it is spare, 80 mesh crush, be added 5 ~ 7 times of raw material weight 60 ~
70% ethanol solution filters under 60 ~ 70 DEG C, 40KHz after 2 ~ 4h of continuous flow upstream ultrasonic extraction, and gained filtrate is concentrated into no alcohol
Taste, it is 1 to be transferred to blade diameter length ratio:4 ~ 6 polyamide resin column, first with the distillation water elution of 2 ~ 3 times of column volumes, afterwards with 4 ~ 6 times of cylinders
70 ~ 80% long-pending ethanol elution, collects eluent, and concentration is dry to get seed of feather cockscomb glycosides A.
The mesh number of the polyamide is 80 mesh.
It is further illustrated the present invention below in conjunction with specific implementation mode, but the scope of protection of present invention is not limited to down
Row embodiment.
Specific implementation mode:
Embodiment 1:
Seed of feather cockscomb medicinal material is cleaned up, the segment of 2-3cm is cut into, it is spare, 80 mesh crush, be added 5 ~ 7 times of raw material weight 60 ~
70% ethanol solution filters under 60 ~ 70 DEG C, 40KHz after 2 ~ 4h of continuous flow upstream ultrasonic extraction, and gained filtrate is concentrated into no alcohol
Taste, it is 1 to be transferred to blade diameter length ratio:The mesh number of 4 ~ 6 polyamide resin column, polyamide is 80 mesh, first with the distilled water of 2 ~ 3 times of column volumes
Elution collects eluent afterwards with 70 ~ 80% ethanol elution of 4 ~ 6 times of column volumes, and concentration is dry to get seed of feather cockscomb glycosides A.
Embodiment 2:
Seed of feather cockscomb medicinal material is cleaned up, the segment of 2-3cm is cut into, it is spare, 80 mesh crush, be added 5 ~ 7 times of raw material weight 60 ~
70% ethanol solution filters under 60 ~ 70 DEG C, 40KHz after 2 ~ 4h of continuous flow upstream ultrasonic extraction, and gained filtrate is concentrated into no alcohol
Taste, it is 1 to be transferred to blade diameter length ratio:The mesh number of 4 ~ 6 polyamide resin column, polyamide is 80 mesh, first with the distilled water of 2 ~ 3 times of column volumes
Elution collects eluent afterwards with 70 ~ 80% ethanol elution of 4 ~ 6 times of column volumes, and concentration is dry to get seed of feather cockscomb glycosides A.
Embodiment 3:
Seed of feather cockscomb medicinal material is cleaned up, the segment of 2-3cm is cut into, it is spare, 80 mesh crush, be added 5 ~ 7 times of raw material weight 60 ~
70% ethanol solution filters under 60 ~ 70 DEG C, 40KHz after 2 ~ 4h of continuous flow upstream ultrasonic extraction, and gained filtrate is concentrated into no alcohol
Taste, it is 1 to be transferred to blade diameter length ratio:The mesh number of 4 ~ 6 polyamide resin column, polyamide is 80 mesh, first with the distilled water of 2 ~ 3 times of column volumes
Elution collects eluent afterwards with 70 ~ 80% ethanol elution of 4 ~ 6 times of column volumes, and concentration is dry to get seed of feather cockscomb glycosides A.
Claims (2)
1. a kind of preparation method of seed of feather cockscomb glycosides A, which is characterized in that include the following steps:Seed of feather cockscomb medicinal material is cleaned up, is cut
It is spare at the segment of 2-3cm, 80 mesh crush, be added 5 ~ 7 times of raw material weight 60 ~ 70% ethanol solution, in 60 ~ 70 DEG C,
It is filtered after 2 ~ 4h of continuous flow upstream ultrasonic extraction under 40KHz, gained filtrate is concentrated into no alcohol taste, and it is 1 to be transferred to blade diameter length ratio:4 ~ 6 it is poly-
Amide resin column is first collected with the distillation water elution of 2 ~ 3 times of column volumes afterwards with 70 ~ 80% ethanol elution of 4 ~ 6 times of column volumes
Eluent, concentration are dry to get seed of feather cockscomb glycosides A.
2. the preparation method of seed of feather cockscomb glycosides A according to claim 1 a kind of, which is characterized in that the mesh of the polyamide
Number is 80 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710227702.7A CN108690114A (en) | 2017-04-10 | 2017-04-10 | A kind of preparation method of seed of feather cockscomb glycosides A |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710227702.7A CN108690114A (en) | 2017-04-10 | 2017-04-10 | A kind of preparation method of seed of feather cockscomb glycosides A |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108690114A true CN108690114A (en) | 2018-10-23 |
Family
ID=63843244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710227702.7A Pending CN108690114A (en) | 2017-04-10 | 2017-04-10 | A kind of preparation method of seed of feather cockscomb glycosides A |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108690114A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114452310A (en) * | 2020-11-10 | 2022-05-10 | 苏州禾研生物技术有限公司 | Plant extract for preventing novel coronavirus infection and preparation method and application thereof |
-
2017
- 2017-04-10 CN CN201710227702.7A patent/CN108690114A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114452310A (en) * | 2020-11-10 | 2022-05-10 | 苏州禾研生物技术有限公司 | Plant extract for preventing novel coronavirus infection and preparation method and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181023 |