CN108689779A - A kind of bioactivity seed dressing and preparation method thereof containing spirulina - Google Patents

A kind of bioactivity seed dressing and preparation method thereof containing spirulina Download PDF

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Publication number
CN108689779A
CN108689779A CN201810662319.9A CN201810662319A CN108689779A CN 108689779 A CN108689779 A CN 108689779A CN 201810662319 A CN201810662319 A CN 201810662319A CN 108689779 A CN108689779 A CN 108689779A
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China
Prior art keywords
bioactivity
seed dressing
spirulina
containing spirulina
weight
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CN201810662319.9A
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CN108689779B (en
Inventor
罗光宏
祖廷勋
王丹霞
陈天仁
杨生辉
陈叶
陈广泉
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Gansu Kaiyuan Biotechnology Development Center Co., Ltd
Hexi University
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Hexi University
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C11/00—Other nitrogenous fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The present invention provides a kind of bioactivity seed dressing and preparation method thereof containing spirulina.The bioactivity seed dressing includes mainly spiral algae-residue, agricultural amino acid original powder, bacteria agent and citric acid;Using the bioactivity seed dressing, it can significantly improve corn and bring the resistance and photosynthetic capacity of stress to adverse circumstance, and the amount of application of phosphate fertilizer and nitrogenous fertilizer can be reduced, reduce the amplitude that soil fertility declines, to further improve the yield of corn.

Description

A kind of bioactivity seed dressing and preparation method thereof containing spirulina
Technical field
The present invention relates to field of agricultural production technologies, more particularly to a kind of bioactivity seed dressing and its system containing spirulina Preparation Method.
Background technology
Corn is the important cereal crops in China and feed resource and the highest cereal crops of processing stage, in recent years, Though the tillage method of straw-returning, which has, the advantages such as increases soil water storage water holding capacity, increases soil permeability, increases soil fertility, But advantageous environment also is provided for the growth and breeding of pest and disease damage simultaneously, in some main maize area, maize brown spot, top rot Disease, rough dwarf disease etc. are developing progressively as Major Diseases, due to using various chemical fertilizer, these diseases to produce various fertilizer and pesticides throughout the year Raw more or less resistance causes the resistivity for the stress that corn brings adverse circumstance poor, to increase the amount ability of pesticide The effect of partial control pest and disease damage is played, and the excessive use of nitrogenous fertilizer and phosphate fertilizer can cause the salinization of soil serious, cause soil It is hardened, influence soil fertility;Simultaneously corn photosynthesis be yield composition material base, by temperature difference, intensity of illumination and The influence of water supply etc., it is therefore, beautiful in the area that the temperature difference is smaller, temperature is more than 40 DEG C or the natural conditions harshnesses such as water supply is low The photosynthesis dramatic decrease of rice, and then the yield of corn is caused significantly to glide.
Invention content
In order to solve corn in the prior art resist adverse circumstance energy force difference and the harsh regional light of condition and can force difference the problems such as, The present invention provides a kind of the bioactivity seed dressing and preparation method thereof containing spirulina, the bioactivity prepared using this method Seed dressing can significantly improve corn and bring the resistance and photosynthetic capacity of stress to adverse circumstance, and can reduce phosphate fertilizer and nitrogen The amount of application of fertilizer reduces the amplitude that soil fertility declines, to further improve the yield of corn.
The present invention provides a kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing master of spirulina It to be prepared by the ingredient of following parts by weight:
It is further improved, which is prepared from the following parts by weight of the components:
It is further improved, the grain size of spiral algae-residue is 0.1-1mm.
Wherein, the spiral algae-residue that the present invention mentions is provided by Gansu Kaiyuan Bio-tech Development Center, spiral algae-residue The mass fraction of middle protein is 35%, and the mass fraction of carotenoid is 0.50%, and the mass fraction of chlorophyll is 1.20%, agricultural amino acid original powder is provided by Jining of Shandong Province Kingsoft bioengineering Co., Ltd product, in agricultural amino acid original powder The mass fraction of amino acid is 37.93%, and the mass fraction that the mass fraction of organic matter is 16.79%, N is 12.24%, P2O5 Mass fraction be 0.84%, K2The mass fraction of O is 1.55%, and citric acid is carried by Wujiang Fu Long Chemical Co., Ltd.s product For;Bioactivity seed dressing agent prescription provided by the invention is reasonable, acts synergistically between each ingredient, can further improve spiral The usage amount of nitrogenous fertilizer is greatly reduced in the nitrogen fixation effect of algae, greatly mitigates pollution of the nitrogenous fertilizer to environment, while can also promote It is decomposed into soil nutrient, improves the activity of soil enzyme, and then improve the respiratory intensity of soil, while plant growth can be promoted to send out It educates, improves crop quality.
It is further improved, the bacteria agent is made of tangerine green trichoderma, raw black streptomycete and solid medium, and the tangerine is green The weight part ratio of trichoderma, the black streptomycete of the life and the solid medium is 0.5-1:1-1.5:30-40.
It is further improved, living bacteria count content >=2,000,000,000/g of the bacteria agent.
Wherein, solid medium is arbitrary solid medium;The present invention is by by tangerine green trichoderma, raw black streptomycete and solid Culture medium is prepared into bacteria agent, and the living bacteria count content for limiting bacteria agent is more than or equal to 2,000,000,000/g, can improve jade Rice brings adverse circumstance the resistance of stress.
It is further improved, bioactivity seed dressing further includes the zinc sulfate of 12.24-20.16 parts by weight, 3.06-5.04 weights Measure the ammonium molybdate of the boric acid and 1.7-2.8 parts by weight of part.
Wherein, zinc sulfate is provided by Gansu Liujia Gorge chemical plant product, and the mass fraction of Zn is in zinc sulfate 16.73%, boric acid is provided by Nanjing Yan Tie trade Co., Ltds product, and the mass fraction of B is 3.18% in boric acid, molybdic acid Ammonium is provided up to industrial chemicals Co., Ltd product by Zhengzhou is abundant, and the mass fraction of Mo is 4.94% in ammonium molybdate;The present invention By the way that zinc sulfate, boric acid, ammonium molybdate are added in bioactivity seed dressing, the physiological disturbance journey of crops seedling stage can be reduced Degree, while corn can be improved again, phytic acid state phosphorus in soil is absorbed and utilized, improve soil activation.
It is further improved, bioactivity seed dressing further includes the brassin lactones and 4.62- of 9.38-11.39 parts by weight The compound sodium nitrophenolate of 5.61 parts by weight.
Wherein, brassin lactones are provided by the Bo Kai biochemical industries Co., Ltd product that breaks a seal, and compound sodium nitrophenolate is by Guangxi Chongzuo Shunda Science and Technology Ltd. of section of city product provides;The present invention in bioactivity seed dressing by being added brassin lactones and multiple nitre Phenol sodium can significantly improve the photosynthetic capacity of corn seedling, still can be into even if making it in the area of condition harshness The sufficient photosynthesis of row, and then improve the yield of corn.
The present invention also provides the preparation method of the bioactivity seed dressing, which includes the following steps:
(1):The zinc sulfate, boric acid, ammonium molybdate of formula ratio are weighed, is adequately uniformly mixed, obtains A mixtures;
(2):The agricultural amino acid original powder of formula ratio, bacteria agent, citric acid are weighed, is adequately uniformly mixed, B mixing is obtained Object;
(3):The brassin lactones and compound sodium nitrophenolate of formula ratio are weighed, is adequately uniformly mixed, obtains C mixtures;
(4) the spiral algae-residue of A mixtures, B mixtures and C mixtures and formula ratio made from step (1)-(3) is abundant Be uniformly mixed to get the bioactivity seed dressing containing spirulina.
Bioactivity seed dressing provided by the present invention containing spirulina, formula is reasonable, acts synergistically between each ingredient, can To reduce amount of application of nitrogen fertilizer, mitigate pollution of the nitrogenous fertilizer to agricultural environment, solves the life that seed production corn critical period of nutrition lacks phosphorus element Serious problem occurs for rationality disease, and mitigates soil to phosphorus fixation, improves soil microbe quantity, promotes soil nutrient It decomposes, improves the activity of soil enzyme, simultaneously, additionally it is possible to improve the resistance that corn brings adverse circumstance stress, and jade can be improved The photosynthetic ability of rice, makes it that can still carry out enough photosynthesis in condition harsh environment, is the growth of corn Energy is provided, and then improves the yield of corn.
Specific implementation mode
With reference to embodiment, the present invention is further described;Following embodiments are illustrative, are not restrictive, Protection scope of the present invention cannot be limited with following embodiments;Equipment used in the present invention is unless otherwise required Common equipment in the art;Method used in the present invention is unless otherwise required common method in the art.
Embodiment 1
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.1mm.
Embodiment 2
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Embodiment 3
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 1mm;
Wherein, the preparation method of the bioactivity seed dressing containing spirulina includes the following steps:
(1):The zinc sulfate, boric acid, ammonium molybdate of formula ratio are weighed, is adequately uniformly mixed, obtains A mixtures;
(2):The agricultural amino acid original powder of formula ratio, bacteria agent, citric acid are weighed, is adequately uniformly mixed, B mixing is obtained Object;
(3):The brassin lactones and compound sodium nitrophenolate of formula ratio are weighed, is adequately uniformly mixed, obtains C mixtures;
(4) the spiral algae-residue of A mixtures, B mixtures and C mixtures and formula ratio made from step (1)-(3) is abundant Be uniformly mixed to get the bioactivity seed dressing containing spirulina.
Embodiment 4
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm;
Wherein, the bacteria agent is made of tangerine green trichoderma, raw black streptomycete and solid medium, the tangerine green trichoderma, The weight part ratio of the black streptomycete of life and the solid medium is 0.5:1:30, the living bacteria count of the bacteria agent Content is 2,100,000,000/g.
Embodiment 5
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm;
Wherein, the bacteria agent is made of tangerine green trichoderma, raw black streptomycete and solid medium, the tangerine green trichoderma, The weight part ratio of the black streptomycete of life and the solid medium is 0.8:1.2:35, effective viable bacteria of the bacteria agent Number content is 2,100,000,000/g.
Embodiment 6
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm;
Wherein, the bacteria agent is made of tangerine green trichoderma, raw black streptomycete and solid medium, the tangerine green trichoderma, The weight part ratio of the black streptomycete of life and the solid medium is 1:1.5:40, the living bacteria count of the bacteria agent Content is 2,100,000,000/g.
Embodiment 7
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Embodiment 8
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Embodiment 9
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Embodiment 10
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.1mm.
Embodiment 11
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Embodiment 12
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Embodiment 13
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.1mm;
Wherein, the bacteria agent is made of tangerine green trichoderma, raw black streptomycete and solid medium, the tangerine green trichoderma, The weight part ratio of the black streptomycete of life and the solid medium is 0.5:1:30, the living bacteria count of the bacteria agent Content is 2,100,000,000/g.
Embodiment 14
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm;
Wherein, the bacteria agent is made of tangerine green trichoderma, raw black streptomycete and solid medium, the tangerine green trichoderma, The weight part ratio of the black streptomycete of life and the solid medium is 0.8:1.2:35, effective viable bacteria of the bacteria agent Number content is 2,100,000,000/g.
Embodiment 15
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 1mm;
Wherein, the bacteria agent is made of tangerine green trichoderma, raw black streptomycete and solid medium, the tangerine green trichoderma, The weight part ratio of the black streptomycete of life and the solid medium is 1:1.5:40, the living bacteria count of the bacteria agent Content is 2,100,000,000/g.
Reference examples 1
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Reference examples 2
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 5mm.
Reference examples 3
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
11.8 bacteria agent 5.6 of agricultural amino acid original powder
Citric acid 2.6;
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Reference examples 4
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Reference examples 5
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
25 bacteria agent 5.6 of spiral algae-residue
Citric acid 2.6;
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Reference examples 6
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm;
Wherein, the bacteria agent is formed by giving birth to black streptomycete and solid medium, the black streptomycete of the life and institute The weight part ratio for stating solid medium is 1.2:35, the living bacteria count content of the bacteria agent is 2,100,000,000/g.
Reference examples 7
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm;
Wherein, the bacteria agent is made of tangerine green trichoderma and solid medium, the tangerine green trichoderma and solid training The weight part ratio for supporting base is 0.8:35, the living bacteria count content of the bacteria agent is 2,100,000,000/g.
Reference examples 8
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm;
Wherein, the bacteria agent is made of trichoderma aureoviride, raw black streptomycete and solid medium, the trichoderma aureoviride, The weight part ratio of the black streptomycete of life and the solid medium is 0.8:1.2:35, effective viable bacteria of the bacteria agent Number content is 2,100,000,000/g.
Reference examples 9
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Reference examples 10
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Reference examples 11
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Reference examples 12
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Reference examples 13
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
Reference examples 14
A kind of bioactivity seed dressing containing spirulina, this contains the bioactivity seed dressing of spirulina by following parts by weight Component is prepared:
Wherein, the grain size of the spiral algae-residue is 0.5mm.
1 nitrogenase activity determination of test example is tested
The bioactivity seed dressing of Example 1-3 and reference examples 1-3 is as experiment 1-3 groups and compares 1-3 groups, and dosage is 2g/kg takes 4 groups as a contrast of the spiral algae-residue of homogenous quantities, takes traditional seed dressing (urea+Diammonium phosphate (DAP)+potassium sulfate of homogenous quantities + zinc sulfate+boric acid+ammonium molybdate=1.82:1.00:1.64:0.13:0.02:0.02) as a contrast 5 groups, each group seed dressing is used Corn seed is handled and is cultivated, corn variety is honest jade 28, and respectively in spinning phase, pustulation period sampling, the method for sampling is: According to the diagonal line method of sampling in experiment cultivation area, 5 sample collection points are determined, it is each to put 10 plants of continuous acquisition, use second Alkynes reduction method carries out the root for the sample adopted the measurement of nitrogenase activity, obtains the nitrogenase activity of each group corn sample root And be averaged, it the results are shown in Table 1.
The nitrogenase activity test result of 1 each group bioactivity seed dressing of table
As can be seen from Table 1, it after the seed cultivation of the bioactivity seed dressing processing of embodiment 1-3, either spins the phase Or pustulation period, the nitrogenase activity of corn root all relatively control 1-5 groups height, it was demonstrated that the present invention by by spiral algae-residue with it is agricultural Amino acid original powder, bacteria agent and citric acid synergistic effect, can significantly improve the nitrogen fixing capacity of corn, reduce nitrogen application Amount mitigates pollution of the nitrogenous fertilizer to agricultural environment, deletes or replaces when exclusive use spirulina or by the spirulina in inventive formulation When changing, the nitrogen fixing capacity of corn seedling can be significantly reduced.
2 soil activation of test example measures experiment
The bioactivity seed dressing of Example 1-3 and reference examples 4-5, dosage 2g/kg, using each group seed dressing to jade Rice seed is handled and is cultivated, and corn variety is honest jade 28, parallel with taking 10 to the seed of each group corn, blade respectively Sample measures respectively:Alpha-amylase, content of reducing sugar in each group corn kernel, soluble sugar content, chlorophyll in blade contain Amount, the root activity of root;10 Duplicate Samples are averaged, and the results are shown in Table 2;
Wherein, alpha-amylase activity is measured using 3,5- dinitrosalicylic acid systems;
Content of reducing sugar is measured using 3,5- dinitrosalicylic acid systems;
Soluble sugar content is measured using anthrone method;
Chlorophyll content is measured using mixed liquor extraction;
Root activity is measured using the method for Bai Baozhang.
The test result that 2 each group seed dressing of table influences soil activation
As shown in Table 2, the indices of embodiment 1-3 are above reference examples 4-5, it was demonstrated that the present invention dresses seed in bioactivity Agricultural amino acid original powder, bacteria agent, citric acid are added in agent, can significantly improve the activity of soil, when deletion or are replaced The indices of any of which ingredient, soil activation can significantly decline.
Test example 3MDA assays are tested
The bioactivity seed dressing of Example 4-6 and reference examples 6-8, dosage 2g/kg, using each group seed dressing to jade Rice seed is handled and is cultivated, and corn variety is honest jade 28, and the blank group of bioactivity seed dressing is not used in another setting, three Leaf phase each group takes 10 blades, root system Duplicate Samples respectively, weighs 0.2g blades, root system, and liquid nitrogen grinding adds at twice 0.05mol·L-1, pH7.8 phosphate buffer 4mL, rinse well, be incorporated into centrifuge tube, be added 0.5% thiobarbituricacidα- 4mL boils 10min in boiling water bath, is immediately placed in cold water, centrifuges 15min under 3000r/min rotating speeds after cooling, takes supernatant Liquid is control with thiobarbituricacidα-, measures absorbance value A532, A600, A450, the results are shown in Table 3.
Influence of the 3 each group seed dressing of table to the MDA contents in corn seedling
As shown in Table 3, the MDA of embodiment 4-6 is below reference examples 6-8 and blank group, it was demonstrated that the present invention is in bioactivity The bacteria agent that seed dressing is prepared using tangerine green trichoderma, raw black streptomycete and solid medium, can significantly reduce maize leaves MDA contents in piece and root system, and then the resistance that corn brings adverse circumstance stress is improved, when deletion or replace in bacteria agent Any ingredient, can lead to the increase of MDA contents in blade and root system.
4 phytase activity of embodiment measures experiment
The bioactivity seed dressing of above example 7-9 and reference examples 9-11, dosage 2g/kg is taken to use each group respectively Seed dressing is handled and is cultivated to corn seed, and corn variety is honest jade 28, and 10 are taken in the tri-leaf period of every group of corn seedling Duplicate Samples measure the phytase activity of its root and are averaged, as a result such as table 4.
The phytase activity test result of 4 inventive samples of table
As shown in Table 4, the seed dressing of embodiment 7-9 is handled and the corn seedling cultivated is at tri-leaf period, the phytic acid of root Enzymatic activity is significantly higher than reference examples 9-11, it was demonstrated that zinc sulfate, boric acid, ammonium molybdate are added in bioactivity seed dressing, it can be with Its phytase activity is significantly improved, and then improves corn and phytic acid state phosphorus in soil is absorbed and utilized, reaches and reduces phosphate fertilizer Amount of application, when deleting or replacing the ingredient in the nutrition regulating agent, it will lead to the reduction of corn root phytase activity.
The measurement of 5 photosynthesis characteristics of test example is tested
The bioactivity seed dressing of Example 10-12 and reference examples 12-14, dosage 2g/kg use each group seed dressing Corn seed is handled and is cultivated, corn variety is honest jade 28, and the blank group of bioactivity seed dressing is not used in another setting With use traditional seed dressing (urea+Diammonium phosphate (DAP)+potassium sulfate+zinc sulfate+boric acid+ammonium molybdate=1.82:1.00:1.64: 0.13:0.02:0.02) traditional seed dressing group, using the portable photosynthetic instrument of II types of CIRAS- 11 under the conditions of fine day:00-13: 00 measures the photosynthesis characteristics of each group corn seedling third blade,;Photosynthesis characteristics include Net Photosynthetic Rate (Pn), transpiration rate (E), Stomatal conductance (Gs) and space between cells CO2Concentration (Ci), determination condition are:LED light source, PAR1600 μm of olm-2·s-1, CO2 A concentration of 360 μm of olmol-1, the results are shown in Table 5.
The photosynthesis characteristics test result of the corn seedling of 5 each group seed dressing of table processing
As shown in Table 5, the every photosynthesis characteristics index and yield of embodiment 10-12 is above reference examples 12-14 and blank Group, it was demonstrated that the present invention adds brassin lactones and compound sodium nitrophenolate in bioactivity seed dressing, can significantly improve corn Photosynthetic capacity makes it that can still carry out enough photosynthesis in condition harsh environment, is provided for the growth of corn Energy.
The measurement of 6 corn yield of test example is tested
The bioactivity seed dressing of Example 13-15, dosage 2g/kg, using each group seed dressing to corn seed into Row is handled and is cultivated, and corn variety is honest jade 28, and another setting is not used the blank group of bioactivity seed dressing and is mixed using tradition Kind agent (urea+Diammonium phosphate (DAP)+potassium sulfate+zinc sulfate+boric acid+ammonium molybdate=1.82:1.00:1.64:0.13:0.02:0.02) Traditional seed dressing group, after harvest corn, measure corn grain number per spike, Ear weight, 100-grain weight and yield respectively, the results are shown in Table 6.
The measurement result of 6 corn yield of table
As shown in Table 6, the indices of the corn of the seed dressing group harvest of embodiment 13-15 are above blank group and tradition Seed dressing group illustrates that the present invention provides the yield that seed dressing can effectively improve corn.
7 spiral algae-residue bioactivity seed dressing product quality analysis result of test example
Quality analysis is carried out to the spiral algae-residue bioactivity seed dressing of the embodiment of the present invention 2, does 10 Duplicate Samples, as a result It is shown in Table 7.
The quality analysis results of the spiral algae-residue bioactivity seed dressing of 7 embodiment 2 of table
As shown in Table 7, spiral algae-residue bioactivity seed dressing provided by the invention reaches the quality requirement of seed dressing.

Claims (10)

1. a kind of bioactivity seed dressing containing spirulina, which is characterized in that be mainly prepared from the following parts by weight of the components:
2. the bioactivity seed dressing containing spirulina as described in claim 1, which is characterized in that by the component of following parts by weight It is prepared:
3. the bioactivity seed dressing containing spirulina as described in claim 1, which is characterized in that the grain size of the spiral algae-residue For 0.1-1mm.
4. the bioactivity seed dressing containing spirulina as described in claim 1, which is characterized in that the bacteria agent is green by tangerine Trichoderma, raw black streptomycete and solid medium composition, the tangerine green trichoderma, the black streptomycete of the life and the solid medium Weight part ratio is 0.5-1:1-1.5:30-40.
5. the bioactivity seed dressing containing spirulina as described in claim 1, which is characterized in that the bacteria agent it is effective Viable count content >=2,000,000,000/g.
6. the bioactivity seed dressing containing spirulina as described in claim 1, which is characterized in that the bioactivity seed dressing Further include the zinc sulfate of 12.24-20.16 parts by weight, 3.06-5.04 parts by weight boric acid and 1.7-2.8 parts by weight ammonium molybdate.
7. the bioactivity seed dressing containing spirulina as described in claim 1, which is characterized in that the bioactivity seed dressing It further include the compound sodium nitrophenolate of the brassin lactones and 4.62-5.61 parts by weight of 9.38-11.39 parts by weight.
8. a kind of preparation method of the bioactivity seed dressing containing spirulina as claimed in claim 5, which is characterized in that described Preparation method includes the following steps:
(1):The zinc sulfate, boric acid, ammonium molybdate of formula ratio are weighed, is adequately uniformly mixed, obtains A mixtures;
(2):The agricultural amino acid original powder of formula ratio, bacteria agent, citric acid are weighed, is adequately uniformly mixed, obtains B mixtures;
(3):The brassin lactones and compound sodium nitrophenolate of formula ratio are weighed, is adequately uniformly mixed, obtains C mixtures;
(4) the spiral algae-residue of A mixtures, B mixtures and C mixtures and formula ratio made from step (1)-(3) is adequately mixed It closes uniformly to get the bioactivity seed dressing containing spirulina.
9. application of the bioactivity seed dressing described in claim 1 containing spirulina in the yield for increasing corn.
10. the bioactivity seed dressing described in claim 1 containing spirulina is in the yield for increasing the corn that kind is honest jade 28 In application.
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101874506A (en) * 2009-04-29 2010-11-03 崔明华 Multifunctional high-efficiency seed dressing agent and preparation method thereof
CN104744171A (en) * 2015-04-01 2015-07-01 山东天威生物科技有限公司 Novel green biological organic fertilizer and preparation method thereof
CN105085111A (en) * 2015-08-12 2015-11-25 生之道生态农业科技股份有限公司 Biological-organic fertilizer special for corn and preparation method thereof
CN107162797A (en) * 2017-07-06 2017-09-15 酒泉农辉农业科技开发有限公司 Sweet-waxy maizes bead algae-residue biology seed dressing fertilizer and preparation method thereof
CN107285918A (en) * 2017-08-10 2017-10-24 英丽华 A kind of biological mixing bacterial manure and its preparation method and application

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101874506A (en) * 2009-04-29 2010-11-03 崔明华 Multifunctional high-efficiency seed dressing agent and preparation method thereof
CN104744171A (en) * 2015-04-01 2015-07-01 山东天威生物科技有限公司 Novel green biological organic fertilizer and preparation method thereof
CN105085111A (en) * 2015-08-12 2015-11-25 生之道生态农业科技股份有限公司 Biological-organic fertilizer special for corn and preparation method thereof
CN107162797A (en) * 2017-07-06 2017-09-15 酒泉农辉农业科技开发有限公司 Sweet-waxy maizes bead algae-residue biology seed dressing fertilizer and preparation method thereof
CN107285918A (en) * 2017-08-10 2017-10-24 英丽华 A kind of biological mixing bacterial manure and its preparation method and application

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