CN108685625A - The artificial acquisition method of pig semen - Google Patents
The artificial acquisition method of pig semen Download PDFInfo
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- CN108685625A CN108685625A CN201710235746.4A CN201710235746A CN108685625A CN 108685625 A CN108685625 A CN 108685625A CN 201710235746 A CN201710235746 A CN 201710235746A CN 108685625 A CN108685625 A CN 108685625A
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- sperm
- dilution
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- diluted
- pig
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/021—Apparatus for collecting seminal fluids; Artificial vaginas
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Reproductive Health (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of artificial acquisition methods of pig semen, include the following steps:1)Prepare before semen collection;2)Semen collection;3)Liquefacient duration;4)Semen dilution;5)Sperm is stored.Acquisition method of the present invention is easy to operate, damage is not small, easy to pollute, is with a wide range of applications, can improve artificial insemination, the pregnant success rate of pig.The sperm for acquiring and storing by the method for the invention, qualification rate is up to 95% or more, and the storage sperm motility of sperm of enabling is good, pregnancy rate is high.
Description
Technical field
The present invention relates to animal productiongs and breeding field, refer in particular to a kind of artificial acquisition method of pig semen.
Background technology
The breeding method of animal is divided into pangamy and hand mating two ways.Pangamy method, which compares, to be suitable for
Groupuscule animal, conveniently saves trouble, but genital diseases easily occur, and then influences pregnancy rate.Technology of artificial insemination is in animal reproduction
Improve a key technology of buck reproductive capacity.The technology of artificial insemination of pig starts from the 30's of 20th century, and China starts from
The 50's of century, but just start to popularize after the nineties.The technology has become the main means of pig breeding breeding at present
One of.The new varieties Multiplication and extension for being conducive to pig using technology of artificial insemination can be reduced to the labor intensity of insemination of sows, section
It about breeds time of consumption, avoids disease caused by male and female pig natural mating, while improving the speed of growth and lean meat of pig
Rate enhances the disease resistance of pig, increases the number of teeming of sow, improves the survival rate of piglet, reduces public swine rearing head number, saves
Feed reduces breeding cost, increases economic efficiency.But in actual mechanical process, because operating method is improper, it be easy to cause essence
Liquid pollutes, and pregnancy rate is low.
Invention content
The purpose of the present invention is being directed to disadvantage present in background technology and problem to be improved and innovated, a boar is provided
The artificial acquisition method of sperm.
The present invention includes the following steps:
1, prepare before semen collection:Dilution is configured first, and is heated up to 37 DEG C;Then the good thermal insulation cup of cleaning and sterilizing is loaded onto into semen collection
Bag filter paper, which is put into insulating box, is preheated to 37 DEG C, while getting out semen collection tool.
2, semen collection:Boar to be adopted is rushed into semen collection room, squeezes boar foreskin urine to the greatest extent, completes boar ejaculation, then will be collected
To sperm be immediately sent to liquefacient duration room.
3, sperm pre-processes:The sperm being collected into is put on constant temperature objective table, sperm heat preservation is carried out;Then sperm is filtered
It goes to carry out quality identification after floccule immediately, discarded to unqualified sperm, qualified sperm is diluted according to sperm concentration.
4, semen dilution:Detection sperm determines extension rate, is then diluted processing, passes through every part of sperm after dilution
In need to ensure that effective sperm quantity is no less than 3,000,000,000;After the completion of dilution, dispensed with semen deposition bottle or insemination-bag, and post label, point
It needs the air in semen deposition bottle or insemination-bag extracting row packing again before dress.
5, sperm is stored:After semen dilution, sperm motility is checked, if without being decreased obviously, dispense by every part of 80ml, add on bottle
Lid sealing;It is transferred directly in 15-20 DEG C of insulating box and stores again after gradually cooling down 1 hour at room temperature prior to 22 DEG C -25 DEG C
It is spare, and preserve during need every 12 hours sperm mixing is primary, prevent sperm precipitate and cause death.
The dilution is made by following methods in one of the embodiments,:Dilution powder 50g is diluted with 1000ml water
To obtain the final product.
Specifically, the dilution powder is Denmark's import dilution powder;The water is distilled water.
The semen dilution method is as follows in one of the embodiments,:Make dilution and sperm in same temperature, temperature
Poor at most no more than 1 DEG C;Dilution is slowly drained into sperm along glass rod or thermometer when dilution, first by sperm with 1:1
Dilution proportion, then repeat and check density and vigor, diluted next time again within 30 minutes after having diluted for the first time, often
It has diluted once, will slowly mix well the sperm in insemination-bag or bottle with dilution.
Advantages of the present invention and advantageous effect:
Acquisition method of the present invention is easy to operate, damage is not small, easy to pollute, is with a wide range of applications, can improve the artificial of pig
Fertilization, pregnant success rate.The sperm for acquiring and storing by the method for the invention, qualification rate is up to 95% or more, the storage sperm of enabling
Motility of sperm is good, pregnancy rate is high.
Specific implementation mode
It is of the invention for ease of understanding, the embodiment of the present invention is shown below.But the present invention can be with many different
Form is realized, however it is not limited to embodiment described herein.Make to the present invention on the contrary, purpose of providing these embodiments is
Disclosure it is more thorough and comprehensive.
Unless otherwise defined, the skill of all technical and scientific terms used herein and the technical field of the present invention
The normally understood meaning of art personnel is identical.Term used in the description is intended merely to describe specifically to implement purpose, is not
It is intended to the limitation present invention.
Embodiment:
One, the preparation before semen collection:
1,1000 milliliters of distilled water are pressed and matches 50 grams of prepared and diluted liquid of Denmark's import dilution powder, be heated up to 37 DEG C.
2, it the good thermal insulation cup of cleaning and sterilizing is loaded onto into breeder's bag filter paper is put into insulating box and be preheated to 37 DEG C.
3, the semen collections tools such as microscope, objective table temperature-constant plate are regulated.
Two, semen collection:
1, boar to be adopted is rushed into semen collection room.
2, semen collection person puts on double gloves, squeezes boar foreskin urine to the greatest extent, is dried after strict sterilization;It is patient when semen collection,
Until boar ejaculates completely.
3, the sperm being collected into is immediately communicated to liquefacient duration room.
Three, liquefacient duration:
1, sperm is put on constant temperature objective table, carries out sperm heat preservation.
2, after sperm being filtered off floccule, quality identification is carried out immediately;Unqualified sperm is discarded, and qualified sperm is according to sperm
Density is diluted, and dilution action should not be too anxious, gradually to carry out.Concrete operations are as follows:
1)Before semen dilution, all utensils are both needed to strict sterilization, drying, and the various vessel touched with sperm and dilution are both needed to
Heating, preheating.
2)Sperm after testing after, determine extension rate, ensure that effectively sperm quantity is no less than 3,000,000,000 in every part of sperm, and need
It dilutes as early as possible.
3)Dilution and sperm are ensured in same temperature, and the temperature difference is at most no more than 1 DEG C.
4)When dilution, dilution is slowly drained into sperm along glass rod or thermometer, sperm cannot be poured into dilution
In, speed cannot be too fast, avoids changing to environment where sperm too big, causes the death of sperm.First by sperm with 1:1 ratio
Then dilution repeats and checks density and vigor, diluted within 30 minutes after having diluted for the first time, often diluted next time again
Once, slowly the sperm in insemination-bag or bottle will be mixed well with dilution.
3, the sperm diluted is bottled by semen deposition requirement, every part of 80 milliliters of sperm, every part of sperm there are 3,000,000,000 sperms.
The quality and disinfection situation of close inspection semen deposition bottle or insemination-bag before packing, underproof bottle or bag abandon without exception not to be had to.Packing
In the process, the air in semen deposition bottle or bag is extracted only, reduces influence of the air to sperm.It is viscous to sperm after packing is good
Good label, to distinguish the number of sperm.
4, after semen dilution, sperm motility is checked, if without being decreased obviously, by every part 80ml packing(1:4 is diluted), bottle
On seal.22 DEG C -25 DEG C of room temperature is first set after 1 hour(Or after being coated with several layers of towels)It is transferred directly to 15-20 DEG C
Insulating box in;Preserve during require every 12 hours sperm mixing is primary, prevent sperm precipitate and cause death.Inspection daily
It looks into Semen routine box temperature degree and is recorded, if the semen quality of storage should be checked by occurring having a power failure comprehensively;Reduce sperm guarantor to the greatest extent
Case on-off times are deposited, in order to avoid cause dead sperm.The sperm that bottling finishes gradually is cooled down at room temperature, not cooled down too fast;
After sperm temperature is down to 17-18 DEG C, it is spare to be put into insulating box.
Embodiment of the present invention is only the description carried out to the preferred embodiment of the present invention, not to the present invention
Conception and scope is defined, and under the premise of not departing from design philosophy of the present invention, engineers and technicians are to this hair in this field
The all variations and modifications that bright technical solution is made should all fall into protection scope of the present invention, the claimed skill of the present invention
Art content has all been recorded in detail in the claims.
Claims (4)
1. a kind of artificial acquisition method of pig semen, it is characterised in that include the following steps:
1)Prepare before semen collection:Dilution is configured first, and is heated up to 37 DEG C;Then the good thermal insulation cup of cleaning and sterilizing is loaded onto into semen collection
Bag filter paper, which is put into insulating box, is preheated to 37 DEG C, while getting out semen collection tool;
2)Semen collection:It boar to be adopted is rushed into semen collection room, squeezes boar foreskin urine to the greatest extent, completes boar ejaculation, then will be collected into
Sperm is immediately sent to liquefacient duration room;
3)Sperm pre-processes:The sperm being collected into is put on constant temperature objective table, sperm heat preservation is carried out;Then sperm is filtered off and is wadded a quilt with cotton
Quality identification is carried out after shape object immediately, discarded to unqualified sperm, qualified sperm is diluted according to sperm concentration;
4)Semen dilution:Detection sperm determines extension rate, processing is then diluted, by being needed in every part of sperm after dilution
Ensure that effective sperm quantity is no less than 3,000,000,000;After the completion of dilution, dispensed with semen deposition bottle or insemination-bag, and post label, before packing
Air in semen deposition bottle or insemination-bag need to be extracted to row packing again;
5)Sperm is stored:After semen dilution, sperm motility is checked, if without being decreased obviously, dispense, be capped on bottle close by every part of 80ml
Envelope;Prior to 22 DEG C -25 DEG C at room temperature gradually cool down 1 hour after be transferred directly to again in 15-20 DEG C of insulating box store it is spare,
And preserve during need every 12 hours sperm mixing is primary, prevent sperm precipitate and cause death.
2. the artificial acquisition method of pig semen according to claim 1, it is characterised in that the dilution is by with lower section
Method is made:Dilution powder 50g is diluted with 1000ml water to obtain the final product.
3. the artificial acquisition method of pig semen according to claim 2, it is characterised in that the dilution powder be Denmark into
Mouth dilution powder;The water is distilled water.
4. the artificial acquisition method of pig semen according to claim 1, it is characterised in that the semen dilution method is such as
Under:
Make dilution and sperm in same temperature, the temperature difference is at most no more than 1 DEG C;By dilution along glass rod or thermometer when dilution
It slowly drains into sperm, first by sperm with 1:Then 1 dilution proportion repeats density and the vigor of checking, dilutes for the first time
Diluted next time again within 30 minutes after complete, often diluted it is primary, will slowly by insemination-bag or bottle sperm with it is dilute
Liquid is released to mix well.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710235746.4A CN108685625A (en) | 2017-04-12 | 2017-04-12 | The artificial acquisition method of pig semen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710235746.4A CN108685625A (en) | 2017-04-12 | 2017-04-12 | The artificial acquisition method of pig semen |
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| Publication Number | Publication Date |
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| CN108685625A true CN108685625A (en) | 2018-10-23 |
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| CN201710235746.4A Pending CN108685625A (en) | 2017-04-12 | 2017-04-12 | The artificial acquisition method of pig semen |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR0160090B1 (en) * | 1995-05-01 | 1999-02-18 | 김광희 | Artificial insemination diluent for liquid sperm of pig |
| CN102579154A (en) * | 2012-03-06 | 2012-07-18 | 平顶山瑞祥牧业有限公司 | Method for artificial impregnation of pig |
| CN103040541A (en) * | 2012-12-17 | 2013-04-17 | 平度市养猪协会 | Artificial insemination method of thoroughbred pig |
| CN105831107A (en) * | 2016-06-04 | 2016-08-10 | 永州市天顺畜牧科技有限责任公司 | Disposal method of porcine semen |
| CN105982765A (en) * | 2015-02-11 | 2016-10-05 | 垫江县优科畜牧有限公司 | Artificial insemination method for pigs |
-
2017
- 2017-04-12 CN CN201710235746.4A patent/CN108685625A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR0160090B1 (en) * | 1995-05-01 | 1999-02-18 | 김광희 | Artificial insemination diluent for liquid sperm of pig |
| CN102579154A (en) * | 2012-03-06 | 2012-07-18 | 平顶山瑞祥牧业有限公司 | Method for artificial impregnation of pig |
| CN103040541A (en) * | 2012-12-17 | 2013-04-17 | 平度市养猪协会 | Artificial insemination method of thoroughbred pig |
| CN105982765A (en) * | 2015-02-11 | 2016-10-05 | 垫江县优科畜牧有限公司 | Artificial insemination method for pigs |
| CN105831107A (en) * | 2016-06-04 | 2016-08-10 | 永州市天顺畜牧科技有限责任公司 | Disposal method of porcine semen |
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Application publication date: 20181023 |