CN108676745B - Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof - Google Patents

Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof Download PDF

Info

Publication number
CN108676745B
CN108676745B CN201810493252.0A CN201810493252A CN108676745B CN 108676745 B CN108676745 B CN 108676745B CN 201810493252 A CN201810493252 A CN 201810493252A CN 108676745 B CN108676745 B CN 108676745B
Authority
CN
China
Prior art keywords
wheat
xylolyticus
rot
microbial inoculum
disease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201810493252.0A
Other languages
Chinese (zh)
Other versions
CN108676745A (en
Inventor
徐海亮
王云鹏
王晓莉
王纪忠
王利国
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhongke Xinrun Shandong Agricultural Technology Development Co ltd
Original Assignee
Huaiyin Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Huaiyin Institute of Technology filed Critical Huaiyin Institute of Technology
Priority to CN201810493252.0A priority Critical patent/CN108676745B/en
Publication of CN108676745A publication Critical patent/CN108676745A/en
Application granted granted Critical
Publication of CN108676745B publication Critical patent/CN108676745B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Forests & Forestry (AREA)
  • Ecology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof. The strain is lysine bacillus xylolyticus (B)Lysinibacillus xylanilyticus) And the strain is preserved in the China general microbiological culture collection center with the preservation date of 2017, 9 and 19 months and the preservation number of CGMCC No. 14645. The biocontrol microbial inoculum prepared by liquid fermentation culture of the LX024 strain has better effect on controlling wheat stem basal rot and gibberellic disease. The biocontrol microbial inoculum LX024 is used in the seedling stage and the growth stage of the wheat to have certain control effects on wheat stem basal rot and gibberellic disease, and the control effects respectively reach 65.01 percent and 65.81 percent.

Description

Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof
Technical Field
The invention belongs to the field of agricultural microorganisms, and relates to a biocontrol lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof.
Background
Wheat scab and basal stem rot are global diseases which are caused by fusarium graminearum and harm wheat, and the yield and the quality of wheat are seriously influenced. The wheat stem basal rot is a compound infectious soil-borne disease which is invaded and mixed by various pathogenic bacteria, and is also called fusarium root rot, fusarium stem basal rot and the like. The disease is a worldwide important disease, and the occurrence of the disease is reported in more than 10 countries. In China, Jiangsu has reported the damage of wheat stem base brown rot, and according to the investigation of a wheat soil-borne disease subject group of plant protection institute of Henan university, the disease is commonly generated in each province of Huang-Huai wheat main producing areas in China. Wheat scab is a devastating disease caused by a complex species of fusarium graminearum that occurs widely in warm, humid and semi-humid regions. The middle and lower wheat areas of Yangtze river and the spring wheat area of east northeast China are the main circulation areas of the disease. In recent years, with global climate change, the incidence of wheat scab has spread to south of Huang-Huai-Dong wheat area. Diseased wheat seriously affects the yield and quality of wheat and also endangers the health of people and livestock.
At present, the prevention and treatment of wheat stem base rot and gibberellic disease mainly adopts the measures of chemical prevention and treatment, breeding disease-resistant varieties, strengthening field management and the like. But chemical control is the most common control method due to the long breeding cycle and the limitation of field management. Although chemical control can effectively control the development of field conditions, secondary pollution is easily caused to the ecological environment and wheat production, so that the environment-friendly biological control technology gradually draws attention. The biological control strategy has the characteristics of wide biological control resource sources, environmental friendliness and the like, and scholars and researchers in various countries and regions actively explore and evaluate the application prospect and potential value of the biological control method in the fusarium graminearum control means. Therefore, the biological control has good production and application values as a novel control measure for wheat scab and stem base rot.
Disclosure of Invention
The invention aims to provide a lysine bacillus xylolyticus LX024 for preventing and treating wheat stalk basal rot and gibberellic disease and application thereof, aiming at the current situation that no biocontrol preparation with higher prevention effect on wheat stalk basal rot and stalk basal rot exists in the prior art.
The purpose of the invention can be realized by the following technical scheme:
the bacterial strain is identified to belong to the lysine bacillus xylolyticus LX024 (Lysinibacillus xylaniilyticus), the biological material sample is preserved and survived, the biological material is classified and named as lysine bacillus xylolyticus LX024, the preservation date is 2017, 9 and 19 days, the preservation unit is China general microbiological culture Collection center (CGMCC), the preservation unit address is No. 3 of Western No.1 of North Cheng of the sunward area in Beijing, and the preservation number is CGMCC No. 14645.
The application of the lysine bacillus xylolyticus LX024 in preventing and treating wheat stem basal rot and gibberellic disease or preparing a biocontrol microbial inoculum for preventing and treating wheat stem basal rot and gibberellic disease.
A biocontrol microbial inoculum for preventing and treating wheat stem basal rot and gibberellic disease comprises the lysine bacillus xylolyticus LX 024. As a preferred technical scheme, the total concentration of viable bacteria in the biocontrol microbial inoculum is not less than 1 multiplied by 108 CFU/mL。
The preparation method of the microbial inoculum comprises the following steps: inoculating the seed bacterial liquid of the lysine bacillus xylolyticus LX024 into an LB fermentation culture medium in a volume ratio of 1:500 for fermentation culture, carrying out shake culture at 28 ℃ for 40-48 h at 150-200 rpm, then centrifuging at 5000-6000 rpm for 10-15 min, taking precipitate to obtain the biocontrol bacterial agent LX024, diluting with sterilized water to prepare the bacterial agent, wherein the total concentration of viable bacteria in the finished bacterial agent is 1 multiplied by 108~1×109 CFU/mL。
Of course, the preparation of the biocontrol microbial inoculum by utilizing the lysine bacillus xylulans LX024 for preventing and treating wheat scab and stalk base rot is not limited to the method. All methods which can culture LX024 in large quantity and keep the biocontrol activity can be used for preparing the biocontrol microbial inoculum claimed by the invention.
The preparation method of the lysine bacillus xylolyticus LX024 seed bacterial liquid with the preservation number of CGMCC No.14645 comprises the following steps: inoculating lysine bacillus xylolyticus LX024 strain with preservation number of CGMCC No.14645 into LB culture solution, culturing at 28 deg.C under shaking at 180rpm until OD value at 600 nm is 0.8, and finishing culturing.
A method for preventing and treating wheat stem base rot and gibberellic disease is to inoculate wheat with the biocontrol microbial inoculum.
After the biocontrol microbial inoculum is diluted by 100-fold and 200-fold, the wheat is respectively inoculated in greenhouse and field environments, and the condition of the disease begins to be counted when the disease occurs. The control effect of the lysine bacillus xylolyticus LX024 on wheat basal stem rot and scab is 65.01% and 65.81% respectively.
The invention has the beneficial effects that:
the invention relates to a biocontrol preparation developed specially for wheat scab and basal stem rot. Because the wheat bran biological agent is a biological agent, a series of problems caused by the use of chemical pesticides are completely avoided, the wheat bran biological agent is beneficial to the pollution-free production of wheat, farmers can not use or reduce the use amount of other chemical pesticides, the expenditure of the farmers can be saved, and the residues of chemical agents are reduced.
Greenhouse experiments and field experiments show that: the lysine bacillus xylolyticus LX024 (CGMCC No. 14645) can remarkably reduce the occurrence of wheat scab and stalk base rot. The biocontrol microbial inoculum prepared by the strain is diluted by 100-200 times for root irrigation treatment, and the control effects on wheat stem basal rot and gibberellic disease can respectively reach 65.01% and 65.81%.
Biological sample preservation information
The lysine bacillus xylolyticus LX024 is classified and named as Lysinibacillus xylolyticus, is preserved in China general microbiological culture Collection center (CGMCC) in 19 th 9 th 2017, and has the preservation address of No. 3 of the Xilu-1-Beijing-oriented region of the sunward area in Beijing, and the preservation number of CGMCC No.14645 of the microbial research institute of the Chinese academy of sciences.
Detailed Description
Example 1
The screening method comprises the following steps: the lysine bacillus for xylose solubilization LX024 (CGMCC No. 14645) is separated from wheat root soil around wharf town in Huaian city, Jiangsu province. Cutting wheat root into 1cm pieces, soaking in 1% sodium hypochlorite for 5min, soaking in 70% ethanol for 1-2 min, and washing with sterile water for 3 times (coating 100 μ l of the final washing solution on R2A solid culture medium for culture, and considering surface sterilization and sterility if aseptic growth occurs after 48 hr). 3 g of the sterilized sample was placed in a bacterial mortar, 27ml of sterile 0.85% NaCl was added, the tissue was macerated, ground and diluted with sterile 0.85% NaCl in a gradient. The dilution of 10-1, 10-2 and 10-3 is smeared on R2A in 100. mu.l each, and cultured at 28 ℃ for 48 h. The largest single colony is selected and inoculated to a fresh R2A solid culture medium, and the purified strain is obtained through repeated transfer. The purified strain was stored in an ultra-low temperature refrigerator at-70 ℃ with 40% glycerol.
The identification method comprises the following steps: the strain is identified through morphological characteristics, physiological and biochemical experiments and 16S rDNA sequence analysis.
Morphological characteristics: gram-positive bacteria, rod-shaped, spore-producing, round colony, light yellow, smooth surface.
Gene amplification and sequence analysis, namely culturing the lysine bacillus xylolyticus LX024 in an LB culture medium at 28 ℃ to a logarithmic phase, centrifuging at 12000 r/min for 5min to collect thalli, extracting the genome DNA of bacteria by adopting a genome DNA rapid extraction kit of Boehringer Bio-engineering (Dalong) Co., Ltd, and amplifying a 16S rDNA gene fragment from the genome DNA by using a 16S rRNA amplification universal primer U8-27 (5 '-AGAGTTTGATC(AC) TGGCTCAG-3'), L1494-1514 (5 '-CTACGG (AG) TACCTTGTTACGAC-3') of the bacteria by using an extracted DNA product as a template. The PCR product is sent to Shanghai biological engineering Co., Ltd for sequencing. Homology comparisons of the determined 16S rRNA gene sequences were performed by BLAST software, and the results were profiled, and therefore, the strain was identified as lysine Bacillus xylolyticus (Lysinibacillus xylolyticus).
TABLE 1 LX024 sequencing alignment results
Bacterial strains Most similar strains and accession numbers Degree of similarity (%)
LX024 Lysinibacillus xylanilyticus KX703024 .1 99
Example 2 preparation of LX024 biocontrol bacterial agent
Inoculating lysine bacillus xylolyticus LX024 (CGMCC No. 14645) strain into LB culture solution, performing shaking culture at 28 ℃ and 180rpm for 16 h, sampling in a super clean bench every 2 h, measuring the OD value at 600 nm, and finishing the culture when the OD value is 0.8, wherein the strain is used as seed strain liquid. Inoculating the seed bacterial liquid into LB fermentation culture liquid according to the volume ratio of 1:500 for fermentation culture, culturing at 28 ℃ and 180rpm for 48h, then centrifuging at 6000 rpm for 10 min, and taking the precipitate to obtain the biocontrol microbial inoculum LX 024. Diluting the biological control agent LX024 with sterilized water to obtain biological control agent with total viable bacteria concentration of 1 × 108~1×109CFU/mL。
Example 3 greenhouse and plot experiments biocontrol bacterium LX024 control Effect on wheat scab and stalk rot
Experiment 1: greenhouse test for detecting the prevention effect of LX024 on wheat stem basal rot (2016.09-2016.12)
A place: huaiyin institute of Industrial and scientific colleges greenhouse
Greenhouse pot experiment: washing wheat seeds twice with sterile water, washing the wheat seeds with 75% alcohol for two minutes for sterilization, then repeatedly washing the wheat seeds with sterile water for three times, and culturing the sterilized wheat seeds in a culture dish for germination. Sowing the seeds into a pot, and inoculating biocontrol bacteria when the seeds grow to the two-leaf one-heart period. After one week, the pathogens are inoculated, and after 7d, observation and statistics are carried out for one month. Culturing three seedlings per pot in a greenhouse (at the temperature of 25 ℃), performing root irrigation treatment on the biocontrol bacterium LX024 bacterium liquid and a control group in a two-leaf one-heart period, repeating the treatment for three times, inoculating pathogenic bacteria after 7 days, performing moisture preservation at the temperature of 25 ℃ for 48h after inoculation, and growing in the greenhouse. The basal stem rot of the wheat stem base is observed and investigated every day.
The wheat stem basal rot investigation is based on the wheat scab test and report technical specification (GB/T15796-.
Grade 0, no obvious symptoms of the outer leaf sheath;
grade 1, first sheath brown rot 1/4 less than the length of the sheath;
grade 2, the first sheath brown wither is smaller than 1/4-1/2 of the length of the sheath;
grade 3, the first sheath brown wither is smaller than 1/2-3/4 of the length of the sheath;
4, the first leaf sheath is completely green and rotten;
grade 5, the third scab has obvious withered brown or dead whole plant,
disease severity (disease index) = Σ (disease progression × number of diseased plants)/(highest disease progression × total number of plants) × 100%
Biocontrol effect = (control group disease severity-treatment group disease severity)/control group disease severity × 100%
Note: calculating disease indexes and control effects of different treatments, and performing difference significance analysis and multiple comparison by using DPS data analysis software.
The investigation result after the biological control agent LX024 is treated for 15 days shows that the biological control effect (hereinafter referred to as control effect) of the biological control agent LX024 on the wheat stem basal rot reaches 65.01 percent (Table 2).
TABLE 2 biocontrol effect of LX024 as biocontrol agent on basal rot of wheat stem
Treatment of Severity of disease% Control effect%
LX024 microbial inoculum 21 .57±2 .33b 65 .01
Control 61 .63±3 .05a ---
Experiment 2: the field plot experiment detects the control effect of LX024 on wheat scab (2016.10-2017.05)
A place: agricultural experimental field of Huaiyin institute of industry and technology
The previous stubble of the test field is corn, and the soil texture is sandy loam. 24 days 10 month 2014: wheat is sown, the sowing density is 100 grains.m < -1 >, the line spacing is 25cm, and 10g of seed fertilizer (compound fertilizer) is applied between two lines; 11/14/2014: thinning wheat to 70-80 m-1; day 11, 23: tillering fertilizer is applied, and 5g of urea is applied in line. Other management is carried out according to a local high-yield cultivation mode in Huaian city.
Test fields 15m × 10m =150m2, and protective rows are planted around the test fields. This trial had 2 treatments, 4 replicates each.
In the test, the wheat variety is selected to be Huai wheat 27; the inoculation period of the gibberella zeae is the flowering period of the wheat, single-flower instillation inoculation is carried out, all varieties are free from diseases before inoculation, 2 florets of the middle florets are inoculated to each ear, 20 ears are inoculated to each treatment cell, and the pathogenic bacteria are inoculated after the biocontrol bacteria LX024 are inoculated and the moisture is kept for 1 d; after 21d, 10 strains were sampled per treatment cell. Controls and each treatment were weighed 4 times.
TABLE 3 grading of wheat scab disease severity
Severity grading Description of the symptoms of head blight disease
0 No obvious disease symptoms at the ear
1 The disease of inoculating spikelet or adjacent spikelets
2 The diseased spikelets account for less than 1/4 of the spike length
3 1/4-1/2 with the disease spike occupying the spike length
4 The diseased spikelets account for over 1/2 of the spike length
Disease severity (disease index) = Σ (disease progression × number of diseased plants)/(highest disease progression × total number of plants) × 100%
Biocontrol effect = (control group disease severity-treatment group disease severity)/control group disease severity × 100%
Note: calculating disease indexes and control effects of different treatments, and performing difference significance analysis and multiple comparison by using DPS data analysis software.
The investigation result 35 days after the 1 st treatment of the biological control agent LX024 shows that the biological control effect (hereinafter referred to as control effect) of the biological control agent LX024 on wheat scab reaches 65.81% (Table 4).
TABLE 4 biocontrol effect of the biocontrol bacterial agent LX024 on wheat scab
Treatment of Severity of disease% Control effect%
LX024 microbial inoculum 20 .94±1 .56b 65 .81
Control 61 .24 ±1 .43a ---

Claims (7)

1. A lysine bacillus xylolyticus LX024 for preventing and treating wheat stalk base rot and gibberellic disease is identified to belong to lysine bacillus xylolyticus (Lysinibacillus xylolyticus), and is preserved in the China general microbiological culture Collection center in 2017 in 9-19 th month, wherein the strain preservation number is CGMCC No. 14645.
2. The application of the lysine bacillus xylolyticus LX024 disclosed in claim 1 in prevention and treatment of wheat basal stem rot and gibberellic disease.
3. The application of the lysine bacillus xylolyticus LX024 disclosed in claim 1 in preparation of a biocontrol microbial inoculum for preventing and treating wheat stem basal rot and gibberellic disease.
4. A biocontrol microbial inoculum for controlling wheat stem basal rot and gibberellic disease, which is characterized in that the biocontrol microbial inoculum comprises the lysine bacillus xylolyticus LX024 in the claim 1.
5. The biocontrol microbial inoculum for controlling wheat stem base rot and gibberellic disease of claim 4, wherein the total viable bacteria concentration in the biocontrol microbial inoculum is not less than 1 x 108 CFU/mL。
6. The use of the biocontrol microbial inoculum of claim 4 or 5 in the control of wheat stem basal rot and head blight.
7. A method for controlling wheat stem basal rot and gibberellic disease, characterized in that wheat is inoculated with the biocontrol microbial inoculum according to claim 4 or 5.
CN201810493252.0A 2018-05-22 2018-05-22 Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof Active CN108676745B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810493252.0A CN108676745B (en) 2018-05-22 2018-05-22 Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810493252.0A CN108676745B (en) 2018-05-22 2018-05-22 Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof

Publications (2)

Publication Number Publication Date
CN108676745A CN108676745A (en) 2018-10-19
CN108676745B true CN108676745B (en) 2021-04-13

Family

ID=63807652

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810493252.0A Active CN108676745B (en) 2018-05-22 2018-05-22 Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof

Country Status (1)

Country Link
CN (1) CN108676745B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111996125B (en) * 2020-08-18 2022-03-04 河南省农业科学院植物保护研究所 Application of chaetomium globosum in preventing and treating wheat stem basal rot
CN113925064B (en) * 2021-11-01 2023-02-03 海南大学 Application of B-lysine-resistant bacillus in inhibition of growth of pitaya soft rot germs
CN114134087B (en) * 2021-12-22 2023-07-14 安徽农业大学 Streptomyces alginolyticus and application thereof in prevention and control of wheat stem basal rot
CN114908012B (en) * 2022-05-19 2022-10-28 中国水产科学研究院黑龙江水产研究所 Bacterial strain beneficial to symbiosis of rice and fish and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106748424A (en) * 2016-11-30 2017-05-31 浙江科技学院 Bamboo grove special bio charcoal base manure material, preparation method and applications

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106748424A (en) * 2016-11-30 2017-05-31 浙江科技学院 Bamboo grove special bio charcoal base manure material, preparation method and applications

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Exploring the Potentials of Lysinibacillus sphaericus ZA9 for Plant Growth Promotion and Biocontrol Activities against Phytopathogenic Fungi;Zakira Naureen et al.;《Frontiers in Microbiology》;20170817;第8卷;第1-11页 *

Also Published As

Publication number Publication date
CN108676745A (en) 2018-10-19

Similar Documents

Publication Publication Date Title
CN108676745B (en) Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof
CN105543132A (en) Bacillus methylotrophicus YB-F7 and application thereof in preventing plant diseases
US20220369648A1 (en) Endophytic falciphora oryzae fo-r20 and its application
CN103981102A (en) DSE (Dark septate endophyte) bacterial strain 24L-4 and application thereof in dendrobium officinale production
CN102108339B (en) Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof
CN109112069B (en) Biocontrol endophytic fungus and application thereof
CN110669691A (en) Bacillus megaterium for preventing and treating plant nematode diseases and application thereof
CN106635921B (en) Bacillus amyloliquefaciens Y15 and its application
CN109749953B (en) Bacillus cereus, microbial inoculum and preparation method and application thereof
CN113151101B (en) Serratia marcescens and application thereof
CN107858300B (en) Bacillus amyloliquefaciens 2YN11 for disease prevention, growth promotion, quality improvement and stress resistance of tomatoes and application thereof
CN111334458B (en) Biocontrol actinomycetes and application thereof in prevention and control of ginger stem basal rot or soybean epidemic disease
CN109609403B (en) Biocontrol bacterium and application thereof in prevention and control of downy mildew of crops
CN101953261B (en) Application of jewel orchid endophytic fungi to jewel orchid seedlings
CN114395485B (en) Adhesive film fungus strain TP-2 capable of promoting stem thickness of dendrobium nobile and application
CN111471620B (en) Biocontrol bacterium BV03, application thereof, microbial inoculum and preparation method of microbial inoculum
CN112646752B (en) Preparation method of microbial preparation
CN109234206B (en) Raoultella ornithinolytica HA011 for preventing and treating rice seedling stage damping-off and application thereof
CN112195120A (en) Biocontrol strain G2C3 for preventing and treating powdery mildew of strawberries and application thereof
CN112430554A (en) Biocontrol bacterium BTh05 for damping-off of melons and fruits, microbial inoculum thereof, preparation method of microbial inoculum and application of microbial inoculum
CN110982764A (en) Bacillus tequilensis S12 for preventing and treating rice blast and application thereof
Long et al. Tiankengomelania guangxiense, gen. et sp. nov., a dark septate endophytic fungus, promotes the growth of the medicinal orchid Dendrobium officinale
CN113151112B (en) Peanut endophytic bacterium burkholderia cepacia R-11 and application thereof
CN111334453B (en) Biocontrol bacterium and application thereof in prevention and control of sheath blight of crops
CN114717139B (en) Oncorhynchus bacteria strain TP-13 with capacity of promoting growth of new dendrobium roots and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20231215

Address after: 271200 No. 512 Binhe Avenue, Xintai Economic Development Zone, Tai'an City, Shandong Province

Patentee after: Zhongke Xinrun (Shandong) Agricultural Technology Development Co.,Ltd.

Address before: 223005 Huaian 1 Jiangsu Economic Development Zone

Patentee before: HUAIYIN INSTITUTE OF TECHNOLOGY