CN108669101A - A kind of Bravo suspending agent and preparation method thereof - Google Patents
A kind of Bravo suspending agent and preparation method thereof Download PDFInfo
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- CN108669101A CN108669101A CN201810604564.4A CN201810604564A CN108669101A CN 108669101 A CN108669101 A CN 108669101A CN 201810604564 A CN201810604564 A CN 201810604564A CN 108669101 A CN108669101 A CN 108669101A
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- suspending agent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/34—Nitriles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/14—Celastraceae [Staff-tree or Bittersweet family], e.g. spindle tree, bittersweet or thunder god vine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
Abstract
The invention discloses a kind of Bravo suspending agents and preparation method thereof, are related to pesticide production technology field, solve existing pesticide and are also easy to produce drug resistance, the technical problem of bactericidal effect difference.Bravo suspending agent includes following components in percentage by weight：Bravo 50 55%；Surfactant 4 6%；Ethylene glycol 3 5%；Aluminium-magnesium silicate 0.1 0.5%；Xanthans 0.1 0.2%；Sodium benzoate 0.05 0.1%；Phyllostachys nigra var. henonis leaf extract 1 3%；Soft water complements to 100%.For Bravo with after Phyllostachys nigra var. henonis leaf extract compounding, synergy can enhance bactericidal effect, reduce the drug resistance of bacterium and fungi, reduce the dosage of Bravo, and Phyllostachys nigra var. henonis abundance can reduce cost, and biological degradability is good, reduce pesticide residue.
Description
Technical field
The present invention relates to pesticide production technology fields, more specifically, it relates to a kind of Bravo suspending agent and its preparation
Method.
Background technology
Bravo is wide spectrum, protective fungicide.The mechanism of action is can be with the glyceraldehyde 3-phosphate dehydrogenation in fungal cell
Enzyme is had an effect, and is combined with the protein containing cysteine in the enzyme, to destroy the enzymatic activity, makes fungal cell's
Metabolism is damaged and loses vitality.Bravo does not have uptake and translocation effect, but after being sprayed onto on plant, can be in body
There is good tackness on table, be not easy to be fallen by rain drop erosion, is mainly used for fruit tree, rust, anthracnose, powdery mildew, frost on vegetables
The prevention of mildew.
It is outstanding that a kind of Bravo of resistance of rainwater washing against is disclosed in the Chinese invention patent of Publication No. CN102630667A
Floating agent, raw material includes the component of following weight percent content：Bravo 40-60%, wetting agent 1-3%, dispersant 2-
5%, synergist 1-5%, thickener 0.1-0.5%, preservative 0.1-0.5%, antifreezing agent 2-10%, suspending agent 0.1-
0.5%, antifoaming agent 0.1-0.5%, surplus is water.
It is one of prevention most effective chemical means of phytopathogen using chemical agent, with the novel pesticide of not cross resistance
It is a good solution to carry out controlling plant diseases.But new drug development is of high cost, the period is long, it is difficult to the upper cause of disease caught up with
The speed that bacterium develops immunity to drugs, other methods such as crop structure adjustment, different pesticide rotations etc., in actual mechanical process,
It is difficult to play apparent effect.
And in contrast, efficient, low toxicity, the compounding of low-residual and mixture are developed and studied with the investment small, lead time
It is short and by attention both domestic and external, people increase development dynamics one after another.
Botanical fungicide is the plant defense element generated using the certain antibacterial materials or induction that contain in some plants,
Kill or effectively inhibit the growth and development of certain pathogens.Antimicrobial compound in plant is a variety of tools that plant generates
The secondary metabolite for having antibacterial activity contains alkaloids, flavonoid class, protein-based, organic acid and phenols chemical combination
Many different types such as object, such as capillin (Capillin), gleditsia sinensis glycoside (Saponin).Once someone reported 1389 kinds of plants
Object is possible as fungicide.Phyllostachys nigra var. henonis extract shows stronger antifungic action, bacterium of the 72h to fusarium graminearum
Silk inhibiting rate is 80% or more.The combination of Bravo and Phyllostachys nigra var. henonis leaf extract has not been reported.
Invention content
In view of the deficienciess of the prior art, the purpose of the present invention one is to provide a kind of Bravo suspending agent, by adopting
With Phyllostachys nigra var. henonis leaf extract and chlorothalonil compound with bactericidal effect, solve the above problems, drop good with bactericidal effect
The advantages of low drug resistance, at low cost, low-residual.
To achieve the above object one, the present invention provides following technical schemes：
A kind of Bravo suspending agent, including following components in percentage by weight：
Bravo 50-55%；
Surfactant 4-6%；
Ethylene glycol 3-5%；
Aluminium-magnesium silicate 0.1-0.5%；
Xanthans 0.1-0.2%；
Sodium benzoate 0.05-0.1%；
Phyllostachys nigra var. henonis leaf extract 1-3%；
Soft water complements to 100%.
By using above-mentioned technical proposal, it has been investigated that, the leaf extract of Phyllostachys nigra var. henonis contains the tools such as alkaloids, quinones
The compound for having antifungic action, to Escherichia coli, staphylococcus aureus, bacillus subtilis, Dipel, aspergillus,
Brewer's yeast has significant inhibition, and is substantially better than mould, brewer's yeast to the inhibition of bacterium；Bravo is
Wide spectrum, protective fungicide.The mechanism of action is to have an effect with the glyceraldehyde 3-phosphate dehydrogenase in fungal cell, with this
The protein containing cysteine is combined in enzyme, to destroy the enzymatic activity, make the metabolism of fungal cell be damaged and
Lose vitality.It has been investigated that Bravo is with after Phyllostachys nigra var. henonis leaf extract compounding, synergy can enhance sterilization effect
Fruit reduces the drug resistance of bacterium and fungi, reduces the dosage of Bravo, and Phyllostachys nigra var. henonis abundance can reduce cost, biology
Degradability is good, reduces pesticide residue.
Further preferably, the Bravo suspending agent further includes common marshmarigold herb stem extraction, the common marshmarigold herb stem extraction
Weight percent be 1-2.5%.
By using above-mentioned technical proposal, have to bacterium, saccharomycete and mould compared with high inhibition in common marshmarigold herb stem extraction
The substance of effect, and, common marshmarigold herb stem extraction and Bravo, Phyllostachys nigra var. henonis stronger than saccharomycete and mould to the bacteriostasis of bacterium
After leaf extract compounding, bactericidal effect can be further promoted.
Further preferably, the Bravo suspending agent further includes Hyssopus officinalis extract, the weight of the Hyssopus officinalis extract
Amount percentage is 1-2.5%.
By using above-mentioned technical proposal, contain the active material for killing fungi and bacterium in Hyssopus officinalis, with Bravo, hair
After golden bamboo extractive compounding, drug effect can be improved, while expanding fungicidal spectrum, reduce dosage, plant disease is made to be difficult to generate
Drug resistance.
Further preferably, the Bravo suspending agent further includes Euonymus japonicus leaf extract, and the Euonymus japonicus leaf carries
It is 2-4% to take the weight percent of object.
It by using above-mentioned technical proposal, is found through experiment, Euonymus japonicus leaf extract can inhibit downy mildew of garpe
Zoosporangium is sprouted, and after Bravo, Phyllostachys nigra var. henonis leaf extract compounding, can be improved drug effect, while expanding fungicidal spectrum, be dropped
Low dosage.
Further preferably, the preparation of the Phyllostachys nigra var. henonis leaf extract includes the following steps：It is derived from the Phyllostachys nigra var. henonis so dried
Leaf 100g is crushed to 50-100 mesh, is added in beaker, and 800-1000mL ethyl acetate is added into beaker, is heated to 50- 60
DEG C, 2-4h is extracted, is filtered while hot, filter residue is removed, obtains Phyllostachys nigra var. henonis leaf extract.
By using above-mentioned technical proposal the second of Phyllostachys nigra var. henonis is found by the selection of selection and parameter to Extraction solvent
Acetoacetic ester extract is stronger to the inhibition of bacterium.
Further preferably, the preparation of the common marshmarigold herb stem extraction includes the following steps：Take fresh horseshoe base of leaf
100g is shredded and is smashed to pieces using tissue mashing machine, and 400-600mL ethyl alcohol is added, and room temperature extracts 20-25h, and filtering removes filter
Slag distills filtrate decompression to 10g, obtains common marshmarigold herb stem extraction.
By using above-mentioned technical proposal horseshoe base of leaf is found by the selection of selection and parameter to Extraction solvent
Ethanol extract is stronger to the bacteriostasis to bacterium.
Further preferably, the preparation of the Hyssopus officinalis extract includes the following steps：Hyssopus officinalis complete stool is fetched, clear water
Soil is cleaned, after Indoor Natural dries, 80 mesh are crushed to pulverizer, Hyssopus officinalis dry powder is made, 100g Hyssopus officinalis dry powder is thrown
Enter in HV XLPE power cables, acetone is added and extracts 10-15h, the pressure in leaching process mesohigh stirred tank gradually rises from 0.2MPa
It is depressed into 0.3-0.5MPa, is filtered, is medicinal extract by filtrate decompression concentration, the amount constant volume of 1mL acetone is added by 1g dry powder, obtains refreshing perfume
Careless extract.
By using above-mentioned technical proposal, it is gradually pressurized with the extraction conducive to active material, by controlling extracting parameter, is adopted
Use acetone as solvent, the Hyssopus officinalis extract of extraction has good inhibiting effect to bacterium, fungi.
Further preferably, the preparation of the Euonymus japonicus leaf extract includes the following steps：Take fresh Euonymus japonicus
Leaf 100g is smashed after cleaning with bruisher, and 500-800mL distilled water is added, and room temperature extracts 20-25h, then ultrasound extraction 0.5-
1h is filtered and is removed solid impurity, is concentrated under reduced pressure as medicinal extract, then be diluted to 1g/mL with distilled water, and the extraction of Euonymus japonicus leaf is obtained
Object.
By using above-mentioned technical proposal, the water extract of Euonymus japonicus leaf sprouts the zoosporangium of downy mildew of garpe
Inhibiting rate is up to 98%.
The purpose of the present invention two is to provide a kind of preparation method of Bravo suspending agent, using hundred bacterium of this method preparation
Clear suspending agent has the advantages that bactericidal effect is good, reduces drug resistance, at low cost, low-residual.
To achieve the above object two, the present invention provides following technical schemes：
A kind of preparation method of Bravo suspending agent, includes the following steps：
Soft water, Bravo, surfactant, ethylene glycol are added in agitated kettle, are uniformly mixed, are mixed by step 1
Object A；
Step 2 is ground in mixture A input sand mills granularity and is less than 2.5 microns, discharging obtains mixture B；
Aluminium-magnesium silicate, xanthans, sodium benzoate, Phyllostachys nigra var. henonis leaf extract are added into mixture B for step 3, are stirred
It is even, obtain Bravo suspending agent.
It is synergistic after Bravo and Phyllostachys nigra var. henonis leaf extract compounding by using above-mentioned technical proposal, it can enhance
Bactericidal effect reduces the drug resistance of bacterium and fungi, reduces the dosage of Bravo, Phyllostachys nigra var. henonis abundance can be reduced into
This, biological degradability is good, reduces pesticide residue.
Further preferably, the step 3 specifically includes：Aluminium-magnesium silicate, xanthans, benzene first are added into mixture B
Sour sodium, Phyllostachys nigra var. henonis leaf extract add common marshmarigold herb stem extraction, Hyssopus officinalis extract, Euonymus japonicus leaf extract, stirring
It is uniformly mixed, obtains Bravo suspending agent.
By using above-mentioned technical proposal, Bravo is extracted with Phyllostachys nigra var. henonis leaf extract, common marshmarigold herb stem extraction, Hyssopus officinalis
It is synergistic after object, Euonymus japonicus leaf extract compounding, while expanding fungicidal spectrum, dosage is reduced, bacterium and fungi are reduced
Drug resistance.
In conclusion compared with prior art, the invention has the advantages that：
(1) present invention is compounded using Bravo and Phyllostachys nigra var. henonis leaf extract, synergistic, can enhance bactericidal effect, is reduced thin
The drug resistance of bacterium and fungi reduces the dosage of Bravo, and Phyllostachys nigra var. henonis abundance can reduce cost, and biological degradability is good,
Reduce pesticide residue.
(2) there is the substance to bacterium, saccharomycete and mould compared with high inhibition effect, and to bacterium in common marshmarigold herb stem extraction
Bacteriostasis it is stronger than saccharomycete and mould, after common marshmarigold herb stem extraction and Bravo, Phyllostachys nigra var. henonis leaf extract compounding, Neng Goujin
One step promotes bactericidal effect.
(3) it is compounded with Bravo, Phyllostachys nigra var. henonis leaf extract containing the active material for killing fungi and bacterium in Hyssopus officinalis
Afterwards, drug effect can be improved, while expanding fungicidal spectrum, dosage is reduced, plant disease is made to be difficult to develop immunity to drugs.
(4) Euonymus japonicus leaf extract can inhibit the zoosporangium of downy mildew of garpe to sprout, with Bravo, Phyllostachys nigra var. henonis
After leaf extract compounding, drug effect can be improved, while expanding fungicidal spectrum, reduce dosage.
Specific implementation mode
With reference to embodiment, the present invention will be described in detail.
Embodiment 1：A kind of Bravo suspending agent, each component and its corresponding weight percent are as shown in table 1, and by with
Lower step prepares：
Soft water, Bravo, surfactant, ethylene glycol are added in agitated kettle, are uniformly mixed, are mixed by step 1
Object A；
Step 2 is ground in mixture A input sand mills granularity and is less than 2.5 microns, discharging obtains mixture B；
Aluminium-magnesium silicate, xanthans, sodium benzoate, Phyllostachys nigra var. henonis leaf extract are added into mixture B for step 3, are stirred
It is even, obtain Bravo suspending agent.
Wherein, the preparation of Phyllostachys nigra var. henonis leaf extract includes the following steps：It is derived from the Phyllostachys nigra var. henonis leaf 100g so dried, is crushed to
50 mesh are added in beaker, and 800mL ethyl acetate is added into beaker, are heated to 50 DEG C, extract 4h, filter while hot, remove filter
Slag obtains Phyllostachys nigra var. henonis leaf extract.
Surfactant is the YUS-FS3000 purchased from Japanese Zhu Ben's Oil Corporation, and Bravo is commercially available quality hundred
Divide than the Bravo active compound for 98%.
Embodiment 2-7：A kind of Bravo suspending agent, difference from example 1 is that, each component and its corresponding weight
It is as shown in table 1 to measure percentage.
Each component and its weight percent in 1 embodiment 1-7 of table
Embodiment 8：A kind of Bravo suspending agent, difference from example 1 is that, each component and its corresponding weight percent
Than as shown in table 2, step 3 specifically includes：Aluminium-magnesium silicate, xanthans, sodium benzoate, Phyllostachys nigra var. henonis leaf are added into mixture B
Extract adds common marshmarigold herb stem extraction, is uniformly mixed, and obtains Bravo suspending agent；
The preparation of common marshmarigold herb stem extraction includes the following steps：Fresh horseshoe base of leaf 100g is taken, shred and uses tissue mashing
Machine is smashed to pieces, and 400mL ethyl alcohol is added, and room temperature extracts 20h, and filtering removes filter residue, filtrate decompression is distilled to 10g, horseshoe is obtained
Base of leaf extract.
Each component and its weight percent in 2 embodiment 8-18 of table
Embodiment 9-10：A kind of Bravo suspending agent, with embodiment 8 the difference is that, each component and its corresponding weight
Percentage is as shown in table 2.
Embodiment 11：A kind of Bravo suspending agent, difference from example 1 is that, each component and its corresponding weight
Amount percentage is as shown in table 2, and step 3 specifically includes：Aluminium-magnesium silicate, xanthans, sodium benzoate, hair are added into mixture B
Golden bamboo extractive adds Hyssopus officinalis extract, is uniformly mixed, and obtains Bravo suspending agent；
The preparation of Hyssopus officinalis extract includes the following steps：Hyssopus officinalis complete stool is fetched, clear water cleans soil, and Indoor Natural dries
Afterwards, 80 mesh are crushed to pulverizer, Hyssopus officinalis dry powder is made, 100g Hyssopus officinalis dry powder is put into HV XLPE power cables, be added third
Ketone extracts 10h, and the pressure in leaching process mesohigh stirred tank gradually boosts to 0.3MPa from 0.2MPa, filters, filtrate is subtracted
Pressure concentration is medicinal extract, and the amount constant volume of 1mL acetone is added by 1g dry powder, obtains Hyssopus officinalis extract.
Embodiment 12-13：A kind of Bravo suspending agent, with embodiment 11 the difference is that, each component and its corresponding
Weight percent it is as shown in table 2.
Embodiment 14：A kind of Bravo suspending agent, with embodiment 12 the difference is that, each component and its corresponding weight
Amount percentage is as shown in table 2, and step 3 specifically includes：Aluminium-magnesium silicate, xanthans, sodium benzoate, hair are added into mixture B
Golden bamboo extractive adds common marshmarigold herb stem extraction, Hyssopus officinalis extract, is uniformly mixed, and obtains Bravo suspension
Agent.
Wherein, the preparation process of common marshmarigold herb stem extraction is same as Example 8, the preparation process and reality of Hyssopus officinalis extract
It is identical to apply example 11.
Embodiment 15：A kind of Bravo suspending agent, difference from example 1 is that, each component and its corresponding weight
Amount percentage is as shown in table 2, and step 3 specifically includes：Aluminium-magnesium silicate, xanthans, sodium benzoate, hair are added into mixture B
Golden bamboo extractive adds Euonymus japonicus leaf extract, is uniformly mixed, and obtains Bravo suspending agent；
The preparation of Euonymus japonicus leaf extract includes the following steps：Fresh Euonymus japonicus leaf 100g is taken, bruisher is used after cleaning
It smashes, 500mL distilled water is added, room temperature extracts 20h, then ultrasound extraction 1h, filters and remove solid impurity, be concentrated under reduced pressure as leaching
Cream, then it is diluted to 1g/mL with distilled water, obtain Euonymus japonicus leaf extract.
Embodiment 16-17：A kind of Bravo suspending agent, with embodiment 15 the difference is that, each component and its corresponding
Weight percent it is as shown in table 2.
Embodiment 18：A kind of Bravo suspending agent, with embodiment 14 the difference is that, each component and its corresponding weight
Amount percentage is as shown in table 2, and step 3 specifically includes：Aluminium-magnesium silicate, xanthans, sodium benzoate, hair are added into mixture B
Golden bamboo extractive adds common marshmarigold herb stem extraction, Hyssopus officinalis extract, Euonymus japonicus leaf extract, is stirred
It is even, obtain Bravo suspending agent；The preparation process of Euonymus japonicus leaf extract is identical as embodiment 15.
Embodiment 19：A kind of Bravo suspending agent, difference from example 1 is that, the system of Phyllostachys nigra var. henonis leaf extract
It is standby to include the following steps：It is derived from the Phyllostachys nigra var. henonis leaf 100g so dried, is crushed to 100 mesh, is added in beaker, is added into beaker
1000mL ethyl acetate is heated to 60 DEG C, extracts 2h, filters while hot, removes filter residue, obtains Phyllostachys nigra var. henonis leaf extract.
Embodiment 20：A kind of Bravo suspending agent, difference from example 1 is that, the system of Phyllostachys nigra var. henonis leaf extract
It is standby to include the following steps：It is derived from the Phyllostachys nigra var. henonis leaf 100g so dried, is crushed to 80 mesh, is added in beaker, is added into beaker
900mL ethyl acetate is heated to 55 DEG C, extracts 3h, filters while hot, removes filter residue, obtains Phyllostachys nigra var. henonis leaf extract.
Embodiment 21：A kind of Bravo suspending agent, with embodiment 8 the difference is that, the system of common marshmarigold herb stem extraction
It is standby to include the following steps：Fresh horseshoe base of leaf 100g is taken, shred and is smashed to pieces using tissue mashing machine, 500mL ethyl alcohol is added,
Room temperature extracts 22h, and filtering removes filter residue, filtrate decompression is distilled to 10g, common marshmarigold herb stem extraction is obtained.
Embodiment 22：A kind of Bravo suspending agent, with embodiment 8 the difference is that, the system of common marshmarigold herb stem extraction
It is standby to include the following steps：Fresh horseshoe base of leaf 100g is taken, shred and is smashed to pieces using tissue mashing machine, 600mL ethyl alcohol is added,
Room temperature extracts 25h, and filtering removes filter residue, filtrate decompression is distilled to 10g, common marshmarigold herb stem extraction is obtained.
Embodiment 23：A kind of Bravo suspending agent, with embodiment 11 the difference is that, the preparation of Hyssopus officinalis extract
Include the following steps：Hyssopus officinalis complete stool is fetched, clear water cleans soil, and after Indoor Natural dries, 80 are crushed to pulverizer
Hyssopus officinalis dry powder is made in mesh, and 100g Hyssopus officinalis dry powder is put into HV XLPE power cables, and acetone is added and extracts 13h, leaching process
Pressure in mesohigh stirred tank gradually boosts to 0.4MPa from 0.2MPa, filters, and is medicinal extract by filtrate decompression concentration, by 1g
Dry powder adds the amount constant volume of 1mL acetone, obtains Hyssopus officinalis extract.
Embodiment 24：A kind of Bravo suspending agent, with embodiment 11 the difference is that, the preparation of Hyssopus officinalis extract
Include the following steps：Hyssopus officinalis complete stool is fetched, clear water cleans soil, and after Indoor Natural dries, 80 are crushed to pulverizer
Hyssopus officinalis dry powder is made in mesh, and 100g Hyssopus officinalis dry powder is put into HV XLPE power cables, and acetone is added and extracts 15h, leaching process
Pressure in mesohigh stirred tank gradually boosts to 0.5MPa from 0.2MPa, filters, and is medicinal extract by filtrate decompression concentration, by 1g
Dry powder adds the amount constant volume of 1mL acetone, obtains Hyssopus officinalis extract.
Embodiment 25：A kind of Bravo suspending agent, with embodiment 15 the difference is that, Euonymus japonicus leaf extract
Preparation includes the following steps：Fresh Euonymus japonicus leaf 100g is taken, is smashed with bruisher after cleaning, 650mL distilled water is added,
Room temperature extracts 22h, then ultrasound extraction 0.75h, filters and removes solid impurity, is concentrated under reduced pressure as medicinal extract, then be diluted to distilled water
1g/mL obtains Euonymus japonicus leaf extract.
Embodiment 26：A kind of Bravo suspending agent, with embodiment 15 the difference is that, Euonymus japonicus leaf extract
Preparation includes the following steps：Fresh Euonymus japonicus leaf 100g is taken, is smashed with bruisher after cleaning, 800mL distilled water is added,
Room temperature extracts 25h, then ultrasound extraction 0.5h, filters and removes solid impurity, is concentrated under reduced pressure as medicinal extract, then be diluted to distilled water
1g/mL obtains Euonymus japonicus leaf extract.
Comparative example 1：A kind of Bravo suspending agent the difference is that, Phyllostachys nigra var. henonis leaf extract is replaced with embodiment 2
For the soft water of equal weight percent.
Comparative example 2：A kind of Bravo suspending agent the difference is that, Phyllostachys nigra var. henonis leaf extract is replaced with embodiment 2
For the Bravo of equal weight percent.
Comparative example 3：A kind of Phyllostachys nigra var. henonis leaf extract, preparation process is same as Example 1, takes and hair gold in embodiment 2
The equal amount of bamboo extractive, it is spare.
Comparative example 4：A kind of Phyllostachys nigra var. henonis leaf extract, the preparation of Phyllostachys nigra var. henonis leaf extract include the following steps：It is derived from right solarization
Dry Phyllostachys nigra var. henonis leaf 100g is crushed to 50 mesh, is added in beaker, and 800mL petroleum ethers are added into beaker, are heated to 50 DEG C,
4h is extracted, is filtered while hot, filter residue is removed, obtains Phyllostachys nigra var. henonis leaf extract, take equal with Phyllostachys nigra var. henonis leaf extract in embodiment 2
Amount, it is spare.
Comparative example 5：A kind of common marshmarigold herb stem extraction, preparation process is same as Example 8, takes and horseshoe in embodiment 9
The equal amount of base of leaf extract, it is spare.
Comparative example 6：A kind of common marshmarigold herb stem extraction, preparation include the following steps：Fresh horseshoe base of leaf 100g is taken,
It shreds and tissue mashing machine is used to smash to pieces, 400mL distilled water is added, room temperature extracts 20h, and filtering removes filter residue, filtrate is subtracted
Pressure distillation obtains common marshmarigold herb stem extraction, takes the amount equal with common marshmarigold herb stem extraction in embodiment 9 to 10g, spare.
Comparative example 7：A kind of Hyssopus officinalis extract, preparation process is identical as embodiment 11, takes fragrant with god in embodiment 12
The equal amount of careless extract, it is spare.
Comparative example 8：A kind of Hyssopus officinalis extract, preparation include the following steps：Hyssopus officinalis complete stool is fetched, clear water is cleaned
After Indoor Natural dries, 80 mesh are crushed to pulverizer for soil, and Hyssopus officinalis dry powder is made, and 100g Hyssopus officinalis dry powder are put into high
It presses in stirred tank, distillation flooding 10h is added, the pressure in leaching process mesohigh stirred tank is gradually boosted to from 0.2MPa
0.3MPa is filtered, and is medicinal extract by filtrate decompression concentration, the amount constant volume of 1mL distilled water is added by 1g dry powder, obtains Hyssopus officinalis extraction
Object takes the amount equal with Hyssopus officinalis extract in embodiment 12, spare.
Comparative example 9：A kind of Euonymus japonicus leaf extract, preparation process is identical as embodiment 15, take in embodiment 16
The equal amount of Euonymus japonicus leaf extract, it is spare.
Comparative example 10：A kind of Euonymus japonicus leaf extract, preparation include the following steps：Take fresh Euonymus japonicus leaf
100g is smashed after cleaning with bruisher, and 500mL methanol is added, and room temperature extracts 20h, then ultrasound extraction 1h, filters and remove solid
Impurity is concentrated under reduced pressure as medicinal extract, and again with methanol is diluted to 1g/mL, obtains Euonymus japonicus leaf extract, take in embodiment 16
The equal amount of Euonymus japonicus leaf extract, it is spare.
Test a field trial
Utilize chemical control downy mildew of garpe made from above-described embodiment and comparative example.
Test specimen：Each 100mL of Bravo suspending agent obtained in Example 1-26, converts tap water 100L, as examination
Sample 1-26 is tested, using each 100mL of sample obtained in comparative example 1-10, converts tap water 100L, as a contrast sample 1- 10.
Test method：Experiment selection carries out in the more serious vineyard of downy mildew over the years, is divided into 36 small
There are 10 plants of vines in area in each cell, before dispenser, after dispenser 10 days in the new climing of each cell random inspection current year extraction,
Whole blades are checked from top to bottom, calculate preventive effect.
Test result：The preventive effect of the downy mildew of garpe of 1-10 pairs of test specimen 1-26 and control sample is as shown in table 3.It will examination
The Comparative result of sample 2 and control sample 1,3 is tested it is found that the preventive effect of test specimen 1 illustrates to use more than control sample 1 and 3
Bravo is compounded with Phyllostachys nigra var. henonis leaf extract, synergistic, can enhance bactericidal effect；
By the Comparative result of test specimen 1-5 and control sample 2 it is found that Phyllostachys nigra var. henonis leaf extract one timing of amount, only increase
The amount of Bravo influences less, and with the increase of Phyllostachys nigra var. henonis leaf extract, to grape downy mildew the preventive effect of downy mildew of garpe
The preventive effect of disease greatly improves, and illustrates that, in the case where ensureing identical preventive effect, the dosage of Bravo can also be reduced；
By the Comparative result of test specimen 2,9,12,14,16,18 and control sample 5,7,9 it is found that common marshmarigold herb stem extraction and
After Bravo, Phyllostachys nigra var. henonis leaf extract compounding, bactericidal effect can be further promoted；Hyssopus officinalis extract and Bravo, Mao Jin
After bamboo extractive compounding, drug effect can be improved；Euonymus japonicus leaf extract can inhibit the zoosporangium of downy mildew of garpe
It sprouts, after Bravo, Phyllostachys nigra var. henonis leaf extract compounding, drug effect can be improved, reduce dosage.Bravo and Phyllostachys nigra var. henonis leaf
It is synergistic after extract, common marshmarigold herb stem extraction, Hyssopus officinalis extract, Euonymus japonicus leaf extract compounding, preventive effect is improved,
Dosage is reduced, the drug resistance of bacterium and fungi is reduced.
Preventive effects of the table 3 test specimen 1-26 and control sample 1-10 to downy mildew of garpe
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-described embodiment,
All technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
For those of ordinary skill, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Bravo suspending agent, which is characterized in that including following components in percentage by weight：
Bravo 50-55%；
Surfactant 4-6%；
Ethylene glycol 3-5%；
Aluminium-magnesium silicate 0.1-0.5%；
Xanthans 0.1-0.2%；
Sodium benzoate 0.05-0.1%；
Phyllostachys nigra var. henonis leaf extract 1-3%；
Soft water complements to 100%.
2. Bravo suspending agent according to claim 1, which is characterized in that the Bravo suspending agent further includes common marshmarigold herb
The weight percent of stem extraction, the common marshmarigold herb stem extraction is 1-2.5%.
3. Bravo suspending agent according to claim 1, which is characterized in that the Bravo suspending agent further includes Hyssopus officinalis
The weight percent of extract, the Hyssopus officinalis extract is 1-2.5%.
4. Bravo suspending agent according to claim 1, which is characterized in that the Bravo suspending agent further includes that Chinese ilex is defended
The weight percent of lance leaf extract, the Euonymus japonicus leaf extract is 2-4%.
5. Bravo suspending agent according to claim 1, which is characterized in that the preparation of the Phyllostachys nigra var. henonis leaf extract includes
Following steps：It is derived from the Phyllostachys nigra var. henonis leaf 100g so dried, is crushed to 50-100 mesh, is added in beaker, 800- is added into beaker
1000mL ethyl acetate is heated to 50-60 DEG C, extracts 2-4h, filters while hot, removes filter residue, obtains Phyllostachys nigra var. henonis leaf extract.
6. Bravo suspending agent according to claim 2, which is characterized in that the preparation of the common marshmarigold herb stem extraction includes
Following steps：Fresh horseshoe base of leaf 100g is taken, shred and is smashed to pieces using tissue mashing machine, 400-600mL ethyl alcohol, room temperature is added
20-25h is extracted, filtering removes filter residue, filtrate decompression is distilled to 10g, common marshmarigold herb stem extraction is obtained.
7. Bravo suspending agent according to claim 3, which is characterized in that the preparation of the Hyssopus officinalis extract include with
Lower step：Hyssopus officinalis complete stool is fetched, clear water cleans soil, and after Indoor Natural dries, 80 mesh are crushed to pulverizer, and god is made
Vanilla dry powder puts into 100g Hyssopus officinalis dry powder in HV XLPE power cables, and acetone is added and extracts 10-15h, leaching process mesohigh stirs
The pressure mixed in kettle gradually boosts to 0.3-0.5MPa from 0.2MPa, filters, and is medicinal extract by filtrate decompression concentration, adds by 1g dry powder
The amount constant volume of 1mL acetone, obtains Hyssopus officinalis extract.
8. Bravo suspending agent according to claim 4, which is characterized in that the preparation packet of the Euonymus japonicus leaf extract
Include following steps：Fresh Euonymus japonicus leaf 100g is taken, is smashed with bruisher after cleaning, 500-800mL distilled water, room temperature is added
20-25h, then ultrasound extraction 0.5-1h are extracted, filters and removes solid impurity, be concentrated under reduced pressure as medicinal extract, then be diluted to distilled water
1g/mL obtains Euonymus japonicus leaf extract.
9. a kind of preparation method of Bravo suspending agent, which is characterized in that include the following steps：
Soft water, Bravo, surfactant, ethylene glycol are added in agitated kettle, are uniformly mixed, are mixed by step 1
Object A；
Step 2 is ground in mixture A input sand mills granularity and is less than 2.5 microns, discharging obtains mixture B；
Aluminium-magnesium silicate, xanthans, sodium benzoate, Phyllostachys nigra var. henonis leaf extract are added into mixture B for step 3, are stirred
It is even, obtain Bravo suspending agent.
10. the preparation method of Bravo suspending agent according to claim 9, which is characterized in that the step 3 is specifically wrapped
It includes：Aluminium-magnesium silicate, xanthans, sodium benzoate, Phyllostachys nigra var. henonis leaf extract are added into mixture B, adds the extraction of horseshoe base of leaf
Object, Hyssopus officinalis extract, Euonymus japonicus leaf extract, are uniformly mixed, and obtain Bravo suspending agent.
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Cited By (1)
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