CN108659950A - A kind of preparation method of Chinese chive seed oil and polypeptide - Google Patents

A kind of preparation method of Chinese chive seed oil and polypeptide Download PDF

Info

Publication number
CN108659950A
CN108659950A CN201810433448.0A CN201810433448A CN108659950A CN 108659950 A CN108659950 A CN 108659950A CN 201810433448 A CN201810433448 A CN 201810433448A CN 108659950 A CN108659950 A CN 108659950A
Authority
CN
China
Prior art keywords
seed
polypeptide
leek seed
leek
chinese chive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810433448.0A
Other languages
Chinese (zh)
Inventor
王立升
周中杰
梁鹏云
刘旭
罗轩
江俊
杨华
陈海燕
林翠梧
吴黎川
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi University
Original Assignee
Guangxi University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi University filed Critical Guangxi University
Priority to CN201810433448.0A priority Critical patent/CN108659950A/en
Publication of CN108659950A publication Critical patent/CN108659950A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C11B1/104—Production of fats or fatty oils from raw materials by extracting using super critical gases or vapours
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/04—Pretreatment of vegetable raw material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)

Abstract

The present invention discloses a kind of preparation method of Chinese chive seed oil and polypeptide, using clean leek seed, with quick-fried in oil crepitation is fried with slow fire, brine is added when frying and processes；With supercritical CO2Abstraction technique process after leek seed, from wherein taking out Chinese chive seed oil；Further using the leek seed after extraction Chinese chive seed oil, the leek seed dregs of rice are crushed into, water and Aspergillus niger liquid state fermentation, last centrifuging and taking filtrate, as leek seed polypeptide extracting solution is added.The leek seed polypeptide that the present invention extracts has preferable stability and diverse biological activities, and the exploitation to developing novel big health-oriented products has great importance with application.

Description

A kind of preparation method of Chinese chive seed oil and polypeptide
Technical field
The invention belongs to natural product chemistries and field of biology.The preparation side of specifically a kind of Chinese chive seed oil and polypeptide Method.
Background technology
Leek is a kind of allium section plant, and there are plantation in all parts of the country.Leek seed is the seed of its drying and ripening.Leek seed Color and luster is more black, and appearance is oblate, and there is wrinkle on surface, and matter is hard, there is leek characteristic odor.With inflammation-diminishing and cough-controlling, eliminating phlegm and relieving asthma, nourishing liver Kidney, warm waist and knee and other effects can be used for treating underbelly pain, the symptoms such as diabetes；So having higher nutritive value and medicinal valence Value.
Leek seed not only contains there are many unsaturated fatty acid oil such as linoleic acid, oleic acid etc., and sulfur compound, alkaloid, Vitamin isoreactivity ingredient.Chinese chive seed oil carries the health-care vegetable oil of leek peat-reek.The method of tradition extraction Chinese chive seed oil There are squeezing method and organic solvent extraction, and both have the disadvantage, squeezing method oil yield is low；Solvent extraction method dissolvent residual and It is unfriendly to environment；The features such as SCF-CO 2 is low, extracting power is strong, efficient with its extraction temperature, has had natural The extraction application study of product essential oil.It is as leek seed preliminary working after leek seed extracts essential oil.Its residue after processing is still It is directly discarded not only to waste resource rich in there is a large amount of protein, while also resulting in environmental pollution.With Life Sci-Tech Rapid development, the research of biologically active polypeptide deepens continuously, and enlightens us and carries out deep processing to leek seed and prepares active peptides Substance.
Present document report is all to extract Chinese chive seed oil and polypeptide from leek seed respectively.The present invention integrates extraction fragrant-flowered garlic Active principle in vegetable seed is of great significance to the surcharge of with improving leek seed deep processing.
Invention content
The object of the present invention is to provide the preparation methods of a kind of Chinese chive seed oil and polypeptide, and this method extraction efficiency is high, to ring Border is friendly, and the surcharge to improving leek seed deep processing plays an important roll.
The technical solution that the present invention solves above-mentioned technical problem is as follows：
A kind of preparation method of Chinese chive seed oil and polypeptide, includes the following steps：
1. taking clean natural leek seed 20kg, when with being fried with slow fire cracker, brine, salt dosage are sprayed when frying 0.4kg, then fried dry are stored in for use at aeration-drying.
2. using supercritical CO2The leek seed cooked is placed in 40L extraction kettles and extracts by abstraction technique, temperature It is 40 DEG C, pressure is 10~30MPa, and separating still temperature is 35 DEG C, and separating still pressure is 10.0MPa, and extraction time is 2~3h, CO2Flow is 2.5~3.0L/h, oil yield 16%~22%.
3. leek seed dry, pulverize the leek seed dregs of rice for 30 mesh after then will be fuel-displaced；Aspergillus niger spore suspension and hair is added Ferment culture medium, it is 1 to adjust pH 3.5, solid-liquid ratio Kg/L:10~1:15 and fermentation time be 48~72h, be then centrifuged for taking filtrate, As leek seed polypeptide extracting solution；It is 635.41~891.58 μ g/mL to measure content of peptides in zymotic fluid according to ninhydrin method.
The preparation process of a kind of above-mentioned Chinese chive seed oil and polypeptide can comprehensively utilize Chinese chive seed oil and polypeptide, realize production Value maximizes.
Advantages of the present invention：The leek seed polypeptide that the present invention extracts has preferable stability and diverse biological activities, right The exploitation and application for developing novel big health-oriented products have great importance.
Specific implementation mode
With reference to embodiment, the invention will be further described.
Embodiment 1
A kind of preparation method of Chinese chive seed oil and polypeptide, includes the following steps：
1. taking net leek seed 20kg, when with being fried with slow fire cracker, brine, salt dosage 0.4kg are sprayed when frying, then fry It is dry, it stores for use at aeration-drying；
It is extracted 2. the leek seed cooked is placed in 40L extraction kettles, temperature is 40 DEG C, pressure 25MPa, separation Kettle temperature degree is 35 DEG C, and separating still pressure is 10.0MPa, extraction time 2h, CO2Flow is 2.5L/h, oil yield 21%；
3. leek seed dry, pulverize the leek seed dregs of rice for 30 mesh after then will be fuel-displaced；Aspergillus niger spore suspension and hair is added Ferment culture medium, it is 1 to adjust pH 3.5, solid-liquid ratio Kg/L:10 and fermentation time be 48h, be then centrifuged for taking filtrate, as leek seed Polypeptide extracting solution；It is 867.53 μ g/mL to measure content of peptides in zymotic fluid according to ninhydrin method.
Embodiment 2
A kind of preparation method of Chinese chive seed oil and polypeptide, includes the following steps：
1. taking net leek seed 20kg, when with being fried with slow fire cracker, brine, salt dosage 0.4kg are sprayed when frying, then fry It is dry, it stores for use at aeration-drying；
It being extracted 2. the leek seed cooked is placed in 40L extraction kettles, temperature is 40 DEG C, extracting pressure 10MPa, Separating still temperature is 35 DEG C, and separating still pressure is 10.0MPa, extraction time 2h, CO2Flow is 2.5L/h, oil yield 16%；
3. leek seed dry, pulverize the leek seed dregs of rice for 30 mesh after then will be fuel-displaced；Aspergillus niger spore suspension and hair is added Ferment culture medium, it is 1 to adjust pH4.5, solid-liquid ratio Kg/L:10 and fermentation time be 48h, be then centrifuged for taking filtrate, as leek seed Polypeptide extracting solution；It is 635.41 μ g/mL to measure content of peptides in zymotic fluid according to ninhydrin method.
Embodiment 3
A kind of preparation method of Chinese chive seed oil and polypeptide, includes the following steps：
1. taking net leek seed 20kg, when with being fried with slow fire cracker, brine, salt dosage 0.4kg are sprayed when frying, then fry It is dry, it stores for use at aeration-drying；
It being extracted 2. the leek seed cooked is placed in 40L extraction kettles, temperature is 40 DEG C, extracting pressure 30MPa, Separating still temperature is 35 DEG C, and separating still pressure is 10.0MPa, extraction time 2h, CO2Flow is 2.5L/h, oil yield 22%；
3. leek seed dry, pulverize the leek seed dregs of rice for 30 mesh after then will be fuel-displaced；Aspergillus niger spore suspension and hair is added Ferment culture medium, it is 1 to adjust pH 2.5, solid-liquid ratio Kg/L:10 and fermentation time be 48h, be then centrifuged for taking filtrate, as leek seed Polypeptide extracting solution；It is 745.35 μ g/mL to measure content of peptides in zymotic fluid according to ninhydrin method.
Embodiment 4
A kind of preparation method of Chinese chive seed oil and polypeptide, includes the following steps：
1. taking net leek seed 20kg, when with being fried with slow fire cracker, brine, salt dosage 0.4kg are sprayed when frying, then fry It is dry, it stores for use at aeration-drying；
It is extracted 2. the leek seed cooked is placed in 40L extraction kettles, temperature is 40 DEG C, pressure 25MPa, separation Kettle temperature degree is 35 DEG C, and separating still pressure is 10.0MPa, extraction time 3h, CO2Flow is 3.0L/h, oil yield 22%；
3. leek seed dry, pulverize the leek seed dregs of rice for 30 mesh after then will be fuel-displaced；Aspergillus niger spore suspension and hair is added Ferment culture medium, it is 1 to adjust pH 3.5, solid-liquid ratio Kg/L:15 and fermentation time be 72h, be then centrifuged for taking filtrate, as leek seed Polypeptide extracting solution；It is 891.58 μ g/mL to measure content of peptides in zymotic fluid according to ninhydrin method.

Claims (2)

1. the preparation method of a kind of Chinese chive seed oil and polypeptide, it is characterised in that：Include the following steps：
(1) clean natural leek seed 20kg being taken, when with being fried with slow fire cracker, spraying brine, salt dosage when frying 0.4kg, then fried dry are stored in for use at aeration-drying；
(2) supercritical CO is used2The leek seed cooked is placed in 40L extraction kettles and extracts by abstraction technique, temperature 40 DEG C, pressure is 10~30MPa, and separating still temperature is 35 DEG C, and separating still pressure is 10.0MPa, and extraction time is 2~3h, CO2Stream Amount is 2.5~3.0L/h, oil yield 16%~22%；
(3) and then by fuel-displaced rear leek seed the leek seed dregs of rice for 30 mesh be dry, pulverize；Aspergillus niger spore suspension is added and fermentation is trained Base is supported, it is 1 to adjust pH 3.5, solid-liquid ratio Kg/L:10~1:15 and fermentation time be 48~72h, be then centrifuged for taking filtrate, as Leek seed polypeptide extracting solution；It is 635.41~891.58 μ g/mL to measure content of peptides in zymotic fluid according to ninhydrin method.
2. the preparation method of a kind of Chinese chive seed oil and polypeptide according to claim 1, it is characterised in that：The technique can incite somebody to action Chinese chive seed oil is comprehensively utilized with polypeptide, realizes maximum output value.
CN201810433448.0A 2018-05-08 2018-05-08 A kind of preparation method of Chinese chive seed oil and polypeptide Pending CN108659950A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810433448.0A CN108659950A (en) 2018-05-08 2018-05-08 A kind of preparation method of Chinese chive seed oil and polypeptide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810433448.0A CN108659950A (en) 2018-05-08 2018-05-08 A kind of preparation method of Chinese chive seed oil and polypeptide

Publications (1)

Publication Number Publication Date
CN108659950A true CN108659950A (en) 2018-10-16

Family

ID=63778162

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810433448.0A Pending CN108659950A (en) 2018-05-08 2018-05-08 A kind of preparation method of Chinese chive seed oil and polypeptide

Country Status (1)

Country Link
CN (1) CN108659950A (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101451089A (en) * 2008-06-04 2009-06-10 南京农业大学 Method for extracting Chinese chive seed oil by supercritical carbon dioxide
CN104804866A (en) * 2015-04-16 2015-07-29 贵州省黎平县黔香园油脂有限公司 Preparation method of rapeseed oil
CN105154217A (en) * 2015-09-24 2015-12-16 全椒县尹氏油脂有限公司 Chinese chive seed oil extracting method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101451089A (en) * 2008-06-04 2009-06-10 南京农业大学 Method for extracting Chinese chive seed oil by supercritical carbon dioxide
CN104804866A (en) * 2015-04-16 2015-07-29 贵州省黎平县黔香园油脂有限公司 Preparation method of rapeseed oil
CN105154217A (en) * 2015-09-24 2015-12-16 全椒县尹氏油脂有限公司 Chinese chive seed oil extracting method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
周丹等: "超临界CO2萃取韭菜籽油的研究", 《化学工程与装备》 *
孙婕，等: "黑曲霉液态发酵韭籽粕提取韭籽多肽工艺", 《食品工业科技》 *

Similar Documents

Publication Publication Date Title
CN106798299A (en) A kind of new turmeric salt and preparation method thereof
CN103484249B (en) Pear-fermented tobacco flavor and preparation method thereof
CN103788218B (en) A kind of Rhizoma Dioscoreae esculentae effective ingredient decomposes extracting method
CN102199486A (en) Method for integrated utilizing sunflower seeds
CN105779131A (en) Method for extracting essential oil from citrus peels
CN105238551A (en) Method for supercritical CO2 extraction and purification of camellia seed oil
CN103409230B (en) Method for low temperature extraction of plant oil through n-butane-ethanol-water double phase solvent
CN105670778A (en) Method for extracting peony seed oil from peony seeds
CN104450397A (en) Preparation method of glutinous rice wine containing dendrobium officinale
CN104095082A (en) Health-care peony seed blend oil and preparation method thereof
CN109593597A (en) A kind of preparation process of healthy tea oil
CN111548852A (en) Fresh-pressing manufacturing process of zanthoxylum oil
CN104845730A (en) Avocado oil extraction method
CN102511569B (en) Production method for extracting allicin oil from garlic
CN101779815A (en) Preparation method of strengthened garlic solid beverage without garlic flavor
CN104547286A (en) Method for preparing Chinese Eaglewood leaf extract
CN112806549A (en) Ginger full-recycling treatment method
CN101869265A (en) Method for using enzyme method for assisting aqueous extraction of insoluble dietary fiber from pollen pini
CN108659950A (en) A kind of preparation method of Chinese chive seed oil and polypeptide
CN105524708A (en) Preparation method of hazelnut oil
CN102399129B (en) Method for extracting 1-octacosanol from bagasse
CN105124575A (en) Stomach strengthening and digestion helping tea seed polypeptide buccal tablet and preparation method thereof
CN104694245A (en) A method for obtaining Chinese wolfberry seed oil by supercritical carbon dioxide extraction
CN106822254A (en) The ultrasonic extraction process of general flavone is extracted from tartary buckwheat shell
CN103229857A (en) Quenching and extracting process of onion oil and garlic oil

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20181016