CN108635449A - One kind disappears silt pain-easing spray and preparation method thereof - Google Patents

One kind disappears silt pain-easing spray and preparation method thereof Download PDF

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CN108635449A
CN108635449A CN201810486405.9A CN201810486405A CN108635449A CN 108635449 A CN108635449 A CN 108635449A CN 201810486405 A CN201810486405 A CN 201810486405A CN 108635449 A CN108635449 A CN 108635449A
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parts
spray
root
component
pain
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叶盛英
沈西宅
李庆云
王媛
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No254 Hospital Pla
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    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K36/328—Commiphora, e.g. mecca myrrh or balm of Gilead
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    • A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • A61K36/716—Clematis (leather flower)
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    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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Abstract

The present invention relates to a kind of utilization rate height,Blood-activating analgetic effect is good,And the one kind for being different from aerosol disappears silt pain-easing spray and preparation method thereof,By safflower,Erythrina bark,The root of Dahurain angelica,Semen momordicae,The root of Chinese clematis,Radix Angelicae Sinensis,Lycopodium calvatum,Myrrh,Frankincense,Pericarpium zanthoxyli,Pawpaw,Rhizoma Chuanxiong forms,Optimize each group distribution ratio,Creative utilization 10000ml,60% ethyl alcohol carries out diacolation,The effect of only needing that using 12 taste Chinese medicines blood-activating analgetic can be reached,And it is spray-type,Dosage form is novel,It is convenient for carrying,It is easy to use,It is significant in efficacy,Toxic side effect is small,Some special outdoor environments are particularly suitable for use,Preferable clinical application effect has been obtained in military training in the army,From in January, 1999 in January, 2000,Use this aerosol therapy traumatic injury,Painful swelling of joints etc. 154,Average analgesic time is 20min,Detumescence 5 days,Dissipate the stasis of blood 5 days.

Description

One kind disappears silt pain-easing spray and preparation method thereof
Technical field
The invention belongs to field of traditional Chinese, it is related to spray formulation, especially one kind and disappears silt pain-easing spray and its preparation Method.
Background technology
Have up to 80% in military training in the army wound for muscle, injured bones and muscles, specific clinical manifestation is local pain, swells It is swollen, have tenderness, the cyanosis of skin, cause daily routines limited when serious." safflower wash " is in military training in the army all the time The common medicine of acute or chronic soft tissue injury.But " safflower wash " is powder of Chinese traditional medicine dosage form, and each use needs boiling water to wash foam washing open.It uses It is inconvenient to carry, and brings difficulty to people's medication, the above problem is especially prominent when especially giving officers' medication of field exercise. It is used for the ease of grassroots army unit, while improving bioavailability, reduce waste.It is necessary to biological profit is made in " safflower wash " The high modern formulation of expenditure, to improve high efficiency, safety and the convenience of clinical application.
Invention content
It is an object of the invention in place of overcome the deficiencies in the prior art, provide, a kind of utilization rate is high, blood-activating analgetic effect One kind that is good and being different from aerosol disappears silt pain-easing spray and preparation method thereof.
The present invention solves its technical problem and following technical scheme is taken to realize：
One kind disappears silt pain-easing spray, and constituent and parts by weight are：1900-2100 parts of safflower, erythrina bark 1400- 1500 parts, 1400-1500 parts of the root of Dahurain angelica, 1400-1500 parts of semen momordicae, 820-840 parts of the root of Chinese clematis, 820-840 parts of Radix Angelicae Sinensis, lycopodium calvatum 450-550 parts, 450-550 parts of myrrh, 450-550 parts of frankincense, 240-260 parts of pericarpium zanthoxyli, 450-550 parts of pawpaw, Rhizoma Chuanxiong 240-260 Part.
Moreover, 2000 parts of safflower, 1488 parts of erythrina bark, 1488 parts of the root of Dahurain angelica, 1488 parts of semen momordicae, 836 parts of the root of Chinese clematis, Radix Angelicae Sinensis 836 parts, 500 parts of lycopodium calvatum, 500 parts of myrrh, 500 parts of frankincense, 252 parts of pericarpium zanthoxyli, 500 parts of pawpaw, 252 parts of Rhizoma Chuanxiong.
A kind of preparation method for the silt pain-easing spray that disappears, is as follows：
(1) smash：Raw medicinal material is ground into coarse powder, crushing and screening is sieved with No. two, and sieve pore internal diameter is 850 μm ± 29 μm, mesh Number be 24 mesh, be uniformly mixed, obtain mixing coarse powder；
(2) diacolation:Step (1) in mixing coarse powder 10000ml be added, infiltrate in 60% ethyl alcohol, be protected from light that closed to place 2 small When, after medicinal material completely moistening, it is packed into percolator, wherein 60% alcohol solvent need to be higher by mixing coarse powder 5cm, then in percolator In it is closed place 48 hours；
(3) collect：Start to collect percolate after 48 hours, 60% ethyl alcohol is added with the drop speed of 80 drops/minute simultaneously, is made molten The liquid level of agent, which remains at, is higher by mixing coarse powder 5cm, after collection percolate reaches 55000ml, stops collecting, stands 48 hours, Supernatant is taken to dispense；
(4) spray processed：Spray is made using spraying apparatus in the supernatant dispensed and relieves pain spraying to get to disappearing to become silted up Medicament is discharged with mist by the pressure of manual pump when use, is sprayed on illing skin by agent.
Moreover, the mixing coarse powder includes component A, B component and component C, preparation process is as follows：
(1) A groups first are made in erythrina bark, the root of Dahurain angelica, the root of Chinese clematis, Radix Angelicae Sinensis, lycopodium calvatum, pawpaw, the filtering of 7 taste pulverizing medicinal materials of Rhizoma Chuanxiong Point；
(2) B component is made in semen momordicae, the filtering of 2 taste pulverizing medicinal materials of pericarpium zanthoxyli again；
(3) frankincense and 2 taste medicinal material of myrrh are smashed into filtering and component C is made；
Finally by safflower crush filtering be gradually added to successively in component C, B component and component A, be uniformly mixed to get Mix coarse powder.
Advantages of the present invention effect：
1, this spray is different from aerosol, and the power that aerosol sprays drug is propellant, (now mostly not such as fluorochloroparaffins With), hydrofluoroalkane, dimethyl ether etc..The characteristics of spray is then a kind of unique dosage form, existing atomized medicine introducing and advantage, and keep away Exempt to use propellant, it is not only safe but also reliable, it can be used for respiratory tract, skin and mucosa or cavity and have a local effect on, can also play whole body work With what is had makees specific use.
2, this spray is suitable for the regional administrations such as skin, mucous membrane, jointed appendage body surface face, cavity, and drug is in fine mist Can go directly site of action, and local concentration is high, work rapid；Dosage is accurate, and dosage is smaller than injecting or taking orally, therefore poison Small side effects, and drug forms local concentration, can reduce pain, and easy to use in misty through lesion.
3, the silt pain-easing spray that disappears of the invention selects TCL is legal to detect the silt analgesic spray that disappears by quality control standard Safflower, the root of Dahurain angelica, Radix Angelicae Sinensis, Rhizoma Chuanxiong in mist agent control the content of hydroxyl safflor yellow A in monarch drug in a prescription safflower using HPLC methods, pass through 10000ml, 60% ethyl alcohol carry out diacolation after, the content of hydroxyl radical carthamin yellow carthamus A is many in the every milliliter of silt pain-easing spray that disappears In 0.35mg, better than other Chinese medicine preparations on the market, meanwhile, according to Acceleration study method in 2005 pharmacopeia annex and combine classical First average rate method, the stability of the silt pain-easing spray that disappears of the present invention is investigated, is placed under the conditions of room temperature (25 DEG C) steady Qualitative good, the term of validity was at 1 year or more.
4, the present invention provides that a kind of utilization rate is high, blood-activating analgetic effect is good and is different from one kind of aerosol and disappears silt analgesic Spray and preparation method thereof, compared to existing preparation, the advantage of this preparation are creative utilization 10000ml, 60% on the market Ethyl alcohol carry out diacolation, it is only necessary to the effect of capable of reaching blood-activating analgetic using 12 taste Chinese medicines, and this preparation is spray-type, dosage form Novelty, existing dosage form only has tincture and emplastrum on the market, and this spray is convenient for carrying, easy to use, significant in efficacy, poison Small side effects are particularly suitable for some special outdoor environments and use, preferable clinic has been obtained in military training in the army and has been answered " safflower wash " treatment traumatic injury, painful swelling of joints etc. 154 are used from January, 1999 in January, 2000 with effect, it is average Analgesic time is 20min, is subsided a swelling 5 days, dissipates the stasis of blood 5 days.
Specific implementation mode
Below by specific embodiment, the invention will be further described, and following embodiment is descriptive, is not limit Qualitatively, protection scope of the present invention cannot be limited with this.
Embodiment 1
One kind disappears silt pain-easing spray, and constituent and parts by weight are：It is 2000 parts of safflower, 1488 parts of erythrina bark, white 1488 parts of the root of Dahurian angelica, 1488 parts of semen momordicae, 836 parts of the root of Chinese clematis, 836 parts of Radix Angelicae Sinensis, 500 parts of lycopodium calvatum, 500 parts of myrrh, 500 parts of frankincense, 252 parts of pericarpium zanthoxyli, 500 parts of pawpaw, 252 parts of Rhizoma Chuanxiong.
Embodiment 2
One kind disappears silt pain-easing spray, and constituent and parts by weight are：It is 1900 parts of safflower, 1400 parts of erythrina bark, white 1400 parts of the root of Dahurian angelica, 1400 parts of semen momordicae, 820 parts of the root of Chinese clematis, 820 parts of Radix Angelicae Sinensis, 450 parts of lycopodium calvatum, 450 parts of myrrh, 450 parts of frankincense, 240 parts of pericarpium zanthoxyli, 450 parts of pawpaw, 240 parts of Rhizoma Chuanxiong.
Embodiment 3
One kind disappears silt pain-easing spray, and constituent and parts by weight are：It is 2100 parts of safflower, 1500 parts of erythrina bark, white 1500 parts of the root of Dahurian angelica, 1500 parts of semen momordicae, 840 parts of the root of Chinese clematis, 840 parts of Radix Angelicae Sinensis, 550 parts of lycopodium calvatum, 550 parts of myrrh, 550 parts of frankincense, 260 parts of pericarpium zanthoxyli, 550 parts of pawpaw, 260 parts of Rhizoma Chuanxiong.
The above-mentioned silt pain-easing spray preparation method that disappears is as follows：
(1) smash：Raw medicinal material is ground into coarse powder, mixing is uniform；
(2) diacolation:(1) step is smashed mixed coarse powder and be added in 10000ml, 60% ethyl alcohol and infiltrated, is protected from light closed put It sets 2 hours, after medicinal material completely moistening, is packed into percolator, wherein 60% alcohol solvent need to be higher by mixing coarse powder 5cm, then ooze It filters closed in cylinder place 48 hours.
(3) collect：Start to collect percolate after 48 hours, 60% ethyl alcohol is added with the drop speed of 80 drops/minute simultaneously, is made molten The liquid level of agent, which remains at, is higher by mixing coarse powder 5cm, after collection percolate reaches 55000ml, stops collecting, stands 48 hours, Supernatant is taken to dispense.
(4) spray processed：Spray is made using spraying apparatus in the supernatant dispensed, by manual pump when use Medicament is discharged with mist, is sprayed on illing skin by pressure.
Above-mentioned mixing coarse powder includes component A, B component and component C, wherein
(1) A groups first are made in erythrina bark, the root of Dahurain angelica, the root of Chinese clematis, Radix Angelicae Sinensis, lycopodium calvatum, pawpaw, the filtering of 7 taste pulverizing medicinal materials of Rhizoma Chuanxiong Point；
(2) B component is made in semen momordicae, the filtering of 2 taste pulverizing medicinal materials of pericarpium zanthoxyli again；
(3) frankincense and 2 taste medicinal material of myrrh are smashed into filtering and component C is made；
(4) safflower is finally crushed filtering to be gradually successively added in component C, B component and component A, be uniformly mixed.
One kind provided by the invention disappears silt pain-easing spray, as a kind of Chinese medicine of independent research of the 254th hospital of liberation army Preparation ratifies preparation code for clinical more than two decades：North word (2006F12010).With activate blood circulation and disperse blood clots, swelling and pain relieving Effect is suitable for traumatic injury, rheumatalgia pain, and muscles and bones is ached, painful swelling of joints etc..It is curative for effect from the present used.Have no bad Reaction occurs.The clinical observation record preserved at present, statistics use the analgesic spray of becoming silted up that disappears from January, 1999 in January, 2000 Traumatic injury, painful swelling of joints etc. 154 are treated in mist agent, and average analgesic time is 20min, subsides a swelling 5 days, dissipates the stasis of blood 5 days, all cases It cures.
Motherland's medicine thinks " impairment of QI resulting in pain, row wound are swollen ", controls and preferably activates blood circulation and disperses blood clots, and swelling and pain relieving, relaxing tendons and activating collaterals keeps its gas logical Freely, swelling and pain are eliminated." safflower wash " is red by the ten pleasant impression Chinese medicinal composition such as safflower, the root of Dahurain angelica, the root of Chinese clematis, lycopodium calvatum, frankincense, myrrh Flower promoting blood circulation is monarch drug in a prescription.Root of Dahurain angelica dispelling wind and eliminating dampness；Semen momordicae dispersing swelling and dissipating binds attack poison；The root of Chinese clematis is pungent scattered and leads to, impatient to be apt to walk, energy Dispelling wind and eliminating dampness, collaterals and relieving pain.This three taste is ministerial drug, helps the work(of safflower promoting blood circulation, and can wind-damp dispelling numbness.Radix Angelicae Sinensis is invigorated blood circulation Blood-activating analgetic；Lycopodium calvatum pungent-warm benefaction, can dispelling wind and eliminating dampness, relaxing tendons and activating collaterals；Frankincense, myrrh promoting blood circulation and stopping pain, subsiding swelling and resolving stagnation.This four taste For adjutant.Pawpaw relaxing tendons and activating collaterals；The sensible qi and blood of Rhizoma Chuanxiong, analgesic therapy of invigorating blood circulation；Pericarpium zanthoxyli is hot, warm in pain, controls wind plug arthritis with fixed pain caused by dampness.This three taste To make medicine.
Medicine safflower contains plurality of active ingredients, as hydroxyl radical carthamin yellow carthamus A (hydroxysaffloryellow A, HSYA), also it is mainly HSYA pharmacology activity research to the pharmacological research of safflower, HSYA, which has, to be inhibited, and thrombosis inflammation is alleviated Disease is reacted, and OH- free radicals can be significantly removed.Promote vascular anastomosis healing the effects that.The ministerial drug root of Dahurain angelica mainly contains perfume Legumin constituents, such as Imperatorin, Isomperatorin have antipyretic, analgesia, anti-inflammatory, resisting pathogenic microbes and promote cell life The effects that long.The research of the ministerial drug root of Chinese clematis is concentrated mainly on protoanemonin and Anemonin, has antibacterial, analgesia, Hang Yanhe Protective effect to Bones and joints.Semen momordicae contains abundant fatty acid, and joint is used in orthopedics and traumatology treatment and plays good dissipating bind Detumescence ding-tong acts on.Lycopodium calvatum has anti-inflammatory, analgesic activity and Immunity regulation.Radix Angelicae Sinensis has anti-inflammatory, analgesia, antiplatelet Aggregation promotees metabolism, eliminates oxygen radical and lipoid peroxidization resistant.Myrrh have anti-inflammatory, analgesia, antibacterial, detumescence and Irritation potential acts on.Frankincense has analgesia, antioxidation.Rhizoma Chuanxiong, Chinese prickly ash play the role of analgesia and inhibit platelet-shaped at.Group Square taste of traditional Chinese medicine has the effects that anti-inflammatory, analgesia, antibacterial, detumescence, inhibition thrombosis, Immunity regulation, basic and mesh mostly Preceding document report for work treatment soft tissue injury drug mechanism be consistent.But the dosage form aging of " safflower wash " in the prior art, Using inconvenient to carry, bioavailability is low, works slow.And the preparation process of medicinal herb spray tends to be ripe, it has also become Chinese medicine system An important branch in agent field.Especially medicinal herb spray is used to medicaments uniformity can be made to disperse when local treatment, is in tiny mist The site of action that can go directly is dripped, local concentration is high, works rapid, can reduce pain, and easy to use.For being better than when local treatment Other dosage forms.
The silt pain-easing spray that disappears of the present invention is by quality control standard, and selecting the legal detections of TCL, " disappear silt analgesic spraying Safflower, the root of Dahurain angelica, Radix Angelicae Sinensis, Rhizoma Chuanxiong in agent " control the content of hydroxyl safflor yellow A in monarch drug in a prescription safflower using HPLC methods, pass through 10000ml, 60% ethyl alcohol carry out diacolation after, the content of hydroxyl radical carthamin yellow carthamus A is not in every milliliter " disappear silt pain-easing spray " Less than 0.35mg.Meanwhile according to Acceleration study method in 2005 pharmacopeia annex and classical first average rate method is combined, to the present invention's The stability of silt pain-easing spray of disappearing is investigated, and shelf-stability is good under the conditions of room temperature (25 DEG C), the term of validity 1 year with On.
Because pain and swelling are main Symptoms after acute soft tissue injury, the silt pain-easing spray that disappears disappears silt analgesic spray Acute soft tissue injury is treated in mist agent, and drug effect is also mainly manifested in anti-inflammatory, analgesia.Inventor is with pharmacological experiment research side Method (pharmacodynamics) evaluation disappears silt pain-easing spray whether there is or not analgesia and to the bioactivity of anti-inflammation.
One, disappear silt pain-easing spray analgesic activity
It investigates its analgesic activity and selects classical analgesic experiment hot plate method in mice, tail-flick method, writhing method.As a result by hot plate method Experimental data two time point latency of pain response of visible control animals are respectively 17.50 ± 6.54 and 13.90 ± 5.43 seconds, Either spray or positive control drug show as the different analgesic activity of intensity after administration, effect in 1 hour after administration Function and effect with obvious effects better than 0.5 hour after administration, 1 hour after administration, the high, medium and low concentration group animal pain of spray Response latency is respectively 21.40 ± 14.12 seconds, 27.30 ± 13.93 seconds and 29.50 ± 18.06 seconds, wherein in, low concentration group P value is less than 0.01 to experimental data compared with the control group, though spray high concentration has analgesic activity, fails to examine by statistics It tests.It is 21.20 ± 3.97 seconds that positive control drug Yunnan Baiyao, which also has specific analgesic activity, latency of pain response, and p value is less than 0.05.By tail-flick method experimental data it is found that 0.5 hour after administration, each administration group animal only shows certain analgesic activity Trend, 1 hour after administration, analgesic activity obviously shows.The each concentration group of spray shows varying degree analgesic activities, The pain sensation response latency of high, medium and low concentration medication animal is respectively 21.40 ± 14.12 seconds, 27.30 ± 13.93 seconds, 29.50 ± 18.06 seconds, value p value compared with control group (pain sensation response latency is 4.86 ± 0.90 seconds) was respectively smaller than 0.001,0.01 With 0.05, positive reference substance Yunnan Baiyao also has stronger analgesic activity.By writhing (pre-) experimental data it is found that the silt analgesia spray that disappears Mist agent does not have analgesic activity to the animal writhing pain reaction caused by glacial acetic acid.Conclusion：Silt pain-easing spray disappear to caused by thermostimulation Sensation of pain has clear antagonism, but does not have specific dose-effect relationship, to chemical stimulation (glacial acetic acid) pain caused sense Feel without antagonism.
Two, disappear silt pain-easing spray anti-inflammatory effect
Investigate the effect of its anti-inflammation.Auricular concha dimethylbenzene inflammation swelling method, capillary permeability experiment have been selected, by The visible control animals swelling degree of auricular concha dimethylbenzene inflammation swelling method experimental data is 8.3 ± 2.9mg, to its swelling after administration The inhibiting effect that degree has intensity different, wherein best with spray low concentration function and effect, swelling is 3.6 ± 2.1, with Control group is less than 0.001 compared to p value, though spray is high, middle concentration has inhibiting effect, fails to pass through since standard deviation is larger Statistical test.It is 5.3 ± 1.8mg that positive control drug Yunnan Baiyao, which also has specific inhibiting effect, swelling, and p value is less than 0.05.By capillary permeability experimental data it is found that each concentration of spray has varying degree that histamine phosphate is inhibited to lure The increased activity of capillary permeability led, the interim function and effect with middle concentration are best, and OD values are 0.099 ± 0.026, P value is less than 0.05 compared with control group (OD values are 0.122 ± 0.023), though high and low concentration group has certain inhibitory activity, Have no statistical significance；Positive reference substance fluocinolone acetonide also has the capillary permeability of stronger inhibition histamine phosphate induction to increase The activity added.Conclusion：The silt pain-easing spray that disappears has the activity of specific confrontation experimental acute inflammation.
Therefore show " disappear silt pain-easing spray " to heat by drug effect zoopery hot plate method in mice, tail-flick method, writhing method Stimulate pain caused feeling that there is clear antagonism, it is suitable with Yunnan Baiyao aerosol analgesic activity, but to chemical stimulation (ice Acetic acid) it is pain caused feeling without antagonism.Drug effect zoopery auricular concha dimethylbenzene inflammation swelling method, capillary permeability are real The activity for showing that " disappear silt pain-easing spray " has specific confrontation experimental acute inflammation is tested, with Yunnan Baiyao aerosol and fluorine Light anti-inflammatory effect is suitable.
Three, the silt pain-easing spray dermatologic safety experiment that disappears is studied
In order to assess its enepidermic safety, we according in relation to toxicity in provisions for new drugs approval requirement and correlation Document offsets silt pain-easing spray and has carried out safety experiment research, and content includes that skin sensibiligen experiment (measures LD50 (half lethal dose) or MTD (maximum tolerated dose)), skin irritation test and skin allergy experiment.
(1) acute toxicity testing result：
Silt pain-easing spray high concentration (large dosage) damaged skin that disappears administration may will produce rat in the secondary work of certain poison With, be mainly manifested in influence rat body weight growth.Since the influence is limited, so only faster in body overall growth rate It is showed in male rat body, and is not showed in the slower female rats body of body weight increase rate.As a result it shows：Disappear silt Pain-easing spray high concentration should use the patient in damaged skin with caution.The silt pain-easing spray rat that disappears is complete and damaged skin is administered Dosage be up to 17.2 (crude drug) g/kg (12 hours, be administered 4 intergal doses) will not animal dead, maximum tolerated dose (MTD) It is 344 times of clinical medicine dose for 17.2 (crude drug) g/kg.
(2) irritant experiment result：
The silt that disappears is showed no erythema and oedema with 6~72h after analgesic group and the administration stimulation of excipient control group, shows that dispersing blood stasis is stopped Analgesic spray and excipient are without skin irritation.According to table 3 and 4 evaluation criterion of table, dispersing blood stasis pain-easing spray total mark mean value It is 0.Stimulus intensity is nonirritant.Conclusion：Spray delivery is to rabbit when a concentration of 0.201g crude drugs/ml of dispersing blood stasis pain-easing spray Skin has no stimulation.
(3) skin allergy experimental result：
After 2,4-dinitrochlorobenzene group animal tested area's self-excitation administration 6h, there are apparent erythema and the oedema, sensitization rate to be 100%；Excipient control group and the silt analgesic group self-excitation that disappears administration after 6~72h be showed no erythema and oedema, show excipient and The silt analgesic that disappears hinders spray without sensitization.
Therefore by skin sensibiligen, skin irritation and skin allergy experiment, show " disappear pain-easing spray " to family Rabbit is nonirritant to rabbit intact skin group without apparent acute toxic reaction, to cavy without cutaneous anaphylaxis.Illustrate this preparation Toxicity is very small, is a kind of preferable external preparation of safety.
Four, hydroxyl radical carthamin yellow carthamus A inhibits the inflammatory reaction of focal cerebral ischemia-reperfusion injury injury rats
The effect experiment silt pain-easing spray that shows to disappear has obviously to the bioactivity of anti-inflammation, therefore inventor will Its effective substance is further studied.Disappear monarch drug in a prescription safflower (Carthamus tinctorius in silt pain-easing spray prescription L.), there is promoting blood circulation latus rectum, removing blood stasis and analgesics and other effects, be usually used in the cards such as vascular occlusion, traumatic injury, sore swell and ache curative.Hydroxyl is red Anthoxanthin (HSYA) is its most important constituent.It is previous statistics indicate that, HSYA can to anti-platelet activating factor with The combination of its receptor.Original cuiture rat layer nerve cell ischemia-reperfusion is damaged during this investigation it turned out, we study HSYA Wound and the effect to transient cerebral ischemia reperfusion injury, and be situated between by leucocyte after studying HSYA treatment ischemia injuries - 1 β of element and expression of tumor necrosis factor-alpha change to illustrate its partial action mechanism.
As a result, it has been found that neural cell injury and internal transient focal cerebral ischemia-reperfusion that HSYA induces hypoxia in vitro It is after inhibiting part focal cerebral ischemia-reperfusion injury to damage due to HSYA that damage, which has neuroprotection, this protective effect, Inflammatory reaction.It is further discovered that HSYA while substantially reducing tumor necrosis factor-alpha and Interleukin -1β in mRNA and protein water Flat expression shows the inflammatory reaction after HSYA inhibits part focal cerebral ischemia-reperfusion injury to damage and inhibits proinflammatory cytokine Effect it is related.
The silt pain-easing spray that disappears developed by series of experiment research substitutes medicine, more as traditional " safflower wash " Increase effect, safe, easy to use, also carrying out clinical trial for " disappear silt pain-easing spray " lays the foundation.Disappear silt analgesic spray at present Mist agent has obtained military medical organization preparation clinical research official written reply, is carrying out clinical research, is promoting the use of.

Claims (4)

  1. The silt pain-easing spray 1. one kind disappears, it is characterised in that：Its constituent and parts by weight are：1900-2100 parts of safflower, sea 1400-1500 parts of paulownia skin, 1400-1500 parts of the root of Dahurain angelica, 1400-1500 parts of semen momordicae, 820-840 parts of the root of Chinese clematis, Radix Angelicae Sinensis 820-840 Part, 450-550 parts of lycopodium calvatum, 450-550 parts of myrrh, 450-550 parts of frankincense, 240-260 parts of pericarpium zanthoxyli, 450-550 parts of pawpaw, river 240-260 parts of rhizome of chuanxiong.
  2. The silt pain-easing spray 2. one kind according to claim 1 disappears, it is characterised in that：Its constituent and parts by weight For：2000 parts of safflower, 1488 parts of the root of Dahurain angelica, 1488 parts of semen momordicae, 836 parts of the root of Chinese clematis, 836 parts of Radix Angelicae Sinensis, stretches muscle at 1488 parts of erythrina bark 500 parts of grass, 500 parts of myrrh, 500 parts of frankincense, 252 parts of pericarpium zanthoxyli, 500 parts of pawpaw, 252 parts of Rhizoma Chuanxiong.
  3. 3. a kind of preparation method for the silt pain-easing spray that disappears, it is characterised in that：It is as follows：
    (1) smash：Raw medicinal material is ground into coarse powder, crushing and screening is sieved with No. two, and sieve pore internal diameter is 850 μm ± 29 μm, and mesh number is 24 mesh are uniformly mixed, and obtain mixing coarse powder；
    (2) diacolation:Step (1) in mixing coarse powder 10000ml be added, infiltrate in 60% ethyl alcohol, be protected from light it is closed place 2 hours, After medicinal material completely moistening after, be packed into percolator, wherein 60% alcohol solvent need to be higher by mixing coarse powder 5cm, then in percolator it is close Close placement 48 hours；
    (3) collect：Start to collect percolate after 48 hours, 60% ethyl alcohol is added with the drop speed of 80 drops/minute simultaneously, makes solvent Liquid level, which remains at, is higher by mixing coarse powder 5cm, after collection percolate reaches 55000ml, stops collecting, stands 48 hours, take Clear liquid dispenses；
    (4) spray processed：Spray is made to get to the silt pain-easing spray that disappears using spraying apparatus in the supernatant dispensed, is made Used time by the pressure of manual pump, medicament is discharged with mist, is sprayed on illing skin.
  4. 4. a kind of preparation method of silt pain-easing spray that disappears according to claim 3, it is characterised in that：The mixing is thick Powder includes component A, B component and component C, and preparation process is as follows：
    (1) component A first is made in erythrina bark, the root of Dahurain angelica, the root of Chinese clematis, Radix Angelicae Sinensis, lycopodium calvatum, pawpaw, the filtering of 7 taste pulverizing medicinal materials of Rhizoma Chuanxiong；
    (2) B component is made in semen momordicae, the filtering of 2 taste pulverizing medicinal materials of pericarpium zanthoxyli again；
    (3) frankincense and 2 taste medicinal material of myrrh are smashed into filtering and component C is made；
    (4) safflower is finally crushed filtering to be gradually successively added in component C, B component and component A, be uniformly mixed to get mixing Coarse powder.
CN201810486405.9A 2018-05-21 2018-05-21 One kind disappears silt pain-easing spray and preparation method thereof Pending CN108635449A (en)

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