CN108627659A - The biochemistry detection equipment and its operating method of thioredoxin reductase detection - Google Patents
The biochemistry detection equipment and its operating method of thioredoxin reductase detection Download PDFInfo
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- CN108627659A CN108627659A CN201710170517.9A CN201710170517A CN108627659A CN 108627659 A CN108627659 A CN 108627659A CN 201710170517 A CN201710170517 A CN 201710170517A CN 108627659 A CN108627659 A CN 108627659A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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Abstract
The invention discloses a kind of biochemistry detection equipment and its operating method for thioredoxin reductase detection, belong to biochemistry detection equipment technical field.The biochemistry detection equipment includes：Storing apparatus, reaction unit, sampling apparatus, agitating device, sensing state, master control system, driving device.The present invention proposes a kind of new full-automatic biochemistry detection equipment, solves the problems, such as to have been manually done thioredoxin reductase detection in people's blood in the prior art, and can improve the detection efficiency that thioredoxin reductase detects in clinic, saving cost.
Description
Technical field
The invention belongs to biochemistry detection equipment technical fields, more particularly to a kind of for thioredoxin reductase in people's blood
(TR) the biochemistry detection equipment and its operating method detected.
Background technology
Thioredoxin reductase (TR) measures the clinical tumor detection project that project is pioneering both at home and abroad.The project can be with
Fill up the blank that paraplasm class medical diagnosis on disease lacks clinical test method.
TR detection devices can be applied to the tumour early screening of Check-up crowd, and the tumor efficiency monitoring of inpatients is multiple
Early warning and health control etc. are sent out, there is the great market demand and development potentiality.
In the prior art, TR detection kits are that primary researches and develops product, and detecting step acts, and flux etc. all has
There is certain limitation, therefore still has improvement and modified space in terms of detection speed and accuracy of detection.
In the implementation of the present invention, inventor has found at least there is following technical problem in the prior art：
It can only be by detecting thioredoxin reductase (TR) in people's blood manually, detection efficiency is low；
The data detected by biochemistry detection equipment in the prior art do not meet the distribution of country data index；
Therefore, it is badly in need of proposing a kind of biochemistry detection equipment of thioredoxin reductase (TR) detection of full-automation,
Data to improve detection efficiency and detect can meet country data index distribution standard.
Invention content
The purpose of the embodiment of the present invention is to provide a kind of realize for thioredoxin reductase (TR) detection in people's blood
Biochemistry detection equipment and its operating method, the present invention propose a kind of new full-automatic biochemistry detection equipment, solve existing
Have and have been manually done in people's blood the problem of thioredoxin reductase detects in technology, and thioredoxin can be improved in clinic also
The detection efficiency of protoenzyme detection saves cost.The present invention realizes " TR Activity determinations " in biochemistry detection equipment for the first time
On full-automatic operation；The data fit country examination criteria requirement directly obtained through the invention, and can guarantee testing number
The warning function (meeting the distribution of country data index) of the detection technique is embodied according to result.
To achieve the above object, one aspect of the present invention provides a kind of life for thioredoxin reductase detection
Change detection device, including：
Storing apparatus surrounds axle center for holding multiple reagents and/or sample, and under the driving effect of driving device
Periodic rotary makes destination agent and/or target sample turn to target liquid filling hole；
Reaction unit periodically turns for holding multiple reaction cups, and under the driving effect of driving device around axle center
It is dynamic that goal response cup is made to turn to destination sample hole；
Sampling apparatus will be from destination sample hole under the driving effect of driving device, surrounding axle center periodic rotary
The destination agent and/or target sample of middle acquisition are added in the corresponding goal response cup of target liquid filling hole；
Sensing state turns for detecting the receiving of reaction rotary state information, storing apparatus of the reaction unit
Dynamic status information, the reagent status information of sampling apparatus；
Master control system is connect with the sensing state, driving device respectively, for based on reaction rotary state letter
Breath accommodates rotary state information and reagent status information, generates and sends corresponding control instruction to driving device；
Driving device connect with the sampling apparatus, the storing apparatus, the reaction unit, is connect for being based on respectively
The control instruction received controls the sampling apparatus, the reaction unit, the storing apparatus and executes corresponding operation.
Another aspect of the present invention provides a kind of operating method of biochemistry detection equipment, including：
When sensing state detects that goal response cup in reaction unit turns to target liquid filling hole and sampling apparatus
When reagent status information is that reagent acquires completion status, reagent addition instruction is generated；
Driving device adds instruction based on the reagent received, and control sampling apparatus turns to the target liquid feeding of reaction unit
Destination agent and/or target sample are simultaneously added in the corresponding goal response cup of target liquid filling hole by hole；
When sensing state detects that destination agent and/or target sample turn to destination sample hole in storing apparatus
And reagent status information be reagent add completion status when, generate reagent acquisition instructions；
Driving device turns to the destination sample of apparatus for placing based on the reagent acquisition instructions received, control sampling apparatus
Destination agent and/or target sample are acquired at hole.
As described above, the embodiment of the present invention proposes, one kind is full automatic to be used for thioredoxin reductase (TR) in people's blood
The biochemistry detection equipment biochemistry detection equipment of detection, realizes the full-automation of biochemistry detection equipment, improves detection efficiency, section
Cost is saved.
Description of the drawings
Fig. 1 is the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention one provides
Structural schematic diagram；
Fig. 2 is the biochemistry detection equipment provided by Embodiment 2 of the present invention for thioredoxin reductase (TR) detection
Structural schematic diagram；
Fig. 2 is the biochemistry detection equipment provided by Embodiment 2 of the present invention for thioredoxin reductase (TR) detection
Structural schematic diagram；
Fig. 3 is the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention ten provides
The flow diagram of operating method；
Fig. 4 is the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention 11 provides
Operating method flow diagram；
Fig. 5 is the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention 12 provides
Operating method flow diagram；
Fig. 6 is the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention 13 provides
Operating method flow diagram；
Fig. 7 is the active cooperation detection equipment of a kind of thioredoxin reductase that the specific example of the present invention provides
Testing principle schematic diagram；
10. storing apparatus, 11. receiving disks, 12. receiving fixing pieces, 20. reaction units, 21. reaction trays, 22. reaction cups are solid
Determine part, 30. sampling apparatuses, 31. sampling revolving parts, 32. sampling fixing pieces, 33. sampling needles, 40. agitating devices, 41. stirring rotations
Turn part, 42. stirring fixing pieces, 43. mixing needles, 50. sensing states, 60. master control systems, 70. driving devices, 80. temperature controls
Device, 90. cleaning devices, 91. hydraulic devices, 92. cleaning assemblies, 100. power-supply system, 100,101. first power subsystem
101,102. second source subsystems, 103. third power subsystems, 104. the 4th power subsystems, 110. clients.
Specific implementation mode
In order to make the objectives, technical solutions and advantages of the present invention clearer, With reference to embodiment and join
According to attached drawing, the present invention is described in more detail.It should be understood that these descriptions are merely illustrative, and it is not intended to limit this hair
Bright range.In addition, in the following description, descriptions of well-known structures and technologies are omitted, to avoid this is unnecessarily obscured
The concept of invention.
The purpose of the embodiment of the present invention is to provide a kind of biochemistry detected for thioredoxin reductase (TR) in people's blood
Detection device, the embodiment of the present invention propose a kind of full automatic biochemistry detection equipment, can improve detection efficiency, save at
This.
Fig. 1 is the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention one provides
Structural schematic diagram.
As shown in Figure 1, the embodiment of the present invention one provides a kind of biochemistry detection equipment, including：Storing apparatus 10, reaction dress
Set 20, sampling apparatus 30, sensing state 50, master control system 60, driving device 70.
Storing apparatus 10 for holding multiple reagents and/or sample, and under the driving effect of driving device 70, surrounds
Axle center periodic rotary makes destination agent and/or target sample turn to target liquid filling hole.
Reaction unit 20 surrounds the axle center period for holding multiple reaction cups, and under the driving effect of driving device 70
Property rotation so that goal response cup turn to destination sample hole.
Sampling apparatus 30, under the driving effect of driving device 70, surrounding axle center periodic rotary, will be adopted from target
The destination agent and/or target sample acquired in sample hole is added in the corresponding goal response cup of target liquid filling hole.
Sensing state 50, for detecting the reaction rotary state information of the reaction unit 20, storing apparatus 10
Accommodate rotary state information, the reagent status information of sampling apparatus 30.
Master control system 60 is connect with the sensing state 50, driving device 70 respectively, for based on reaction rotation shape
State information accommodates rotary state information and reagent status information, generates and sends corresponding control instruction to driving device 70；
Driving device 70 is connect with the sampling apparatus 30, the storing apparatus 10, the reaction unit 20 respectively, is used
In based on the control instruction received, controls the sampling apparatus 30, the reaction unit 20, the storing apparatus 10 and execute phase
The operation answered.
Fig. 2 is the biochemistry detection equipment provided by Embodiment 2 of the present invention for thioredoxin reductase (TR) detection
Structural schematic diagram.
Storing apparatus 10 includes：Disk 11 is accommodated, activity setting does periodic rotary around axle center.Accommodating has on disk 11
Multiple receiving fixing pieces 12 are used for containing reagent bottle and/or specimen bottle.
Optionally, at least one circle is set on accommodating disk 11 and accommodates fixing piece 12, often circle has multiple receiving fixing pieces 12.
Accommodate fixing piece 12 number can need to carry out select and set according to the specific of user.
Preferably, multiple receiving fixing pieces 12 can be arranged to be uniformly arranged along the edge for accommodating disk 11, i.e., each two accommodates
It is spaced a predetermined distance between fixing piece 12, forms at least one circle being uniformly arranged and accommodate fixing piece 12.
For each receiving fixing piece 12, reagent bottle holder can be accommodated or accommodate sample bottle holder, when needing to increase
When being loaded one's own department or unit reduction reagent position, reagent bottle holder can be removed, change sample bottle holder；Sample is reduced when needing increase reagent position
When position, then sample bottle holder can be removed and change reagent bottle holder, realize the flexible exchange of reagent position and sample bit, Neng Gouman
Different demands of the sufficient different clients to sample bit and reagent position.
As shown in Fig. 2, the reaction unit 20 includes：Reaction tray 21, activity setting, periodic rotary is done around axle center.
Multiple reaction cup fixing pieces 22, for holding reaction cup.
Optionally, at least one circle reaction cup fixing piece 22 is set in reaction tray 21, and often there are circle multiple reaction cups to fix
Part 22.Reaction cup fix 22 number can need to carry out select and set according to the specific of user.
Preferably, multiple reaction cup fixing pieces 22 can be arranged to be uniformly arranged along the edge of reaction tray 21, i.e., each two is anti-
It answers and is spaced a predetermined distance between glass fixing piece 22, form at least one circle reaction cup fixing piece 22 being uniformly arranged.
As shown in Fig. 2, sampling apparatus 30 includes：Sample revolving part 31, activity setting, for the driving in driving device 70
Under effect, axle center periodic rotary is surrounded；Fixing piece 32 is sampled, is fixed on sampled rotational part 31, in sampling revolving part
It is rotated under 32 induced effect；Sampling needle 33, one end are fixing end, and the other end is free end.
In conjunction with Fig. 1,2, illustrate reagent adding procedure：
Reaction rotary state information and examination of the master control system 60 when receiving goal response cup and turning to target liquid filling hole
When agent status information is that reagent acquires completion status, reagent addition instruction is generated；The driving device 70 adds receiving reagent
After adding instruction, control sampling apparatus 30 turns to the target liquid filling hole of reaction unit 20 and by destination agent and/or target sample
It is added in the corresponding goal response cup of target liquid filling hole.
In conjunction with Fig. 1,2, illustrate reagent gatherer process：
Receiving of the master control system 60 when receiving destination agent and/or whether target sample turns to destination sample hole turns
When dynamic status information and when reagent status information is that reagent adds completion status, reagent acquisition instructions are generated；The driving device
70 after receiving reagent acquisition instructions, and control sampling apparatus 30, which turns to, acquires target at the destination sample hole of apparatus for placing 10
Reagent and/or target sample.
What the embodiment of the present invention three provided, which is used for the biochemistry detection equipment that thioredoxin reductase (TR) detects, further includes：
Agitating device 40, under the driving effect of driving device 70, periodic rotary to be done in goal response cup around axle center
The mixed liquor of formation is stirred.
As shown in Fig. 2, the agitating device 40 includes：Stir revolving part 41, fixed setting, in driving device 70
Under driving effect, axle center periodic rotary is surrounded；Fixing piece 42 is stirred, is fixed on stirring rotating member 31, is revolved in stirring
Turn to rotate under the induced effect of part 42；Mixing needle 43, one end are fixing end, and the other end is free end.
In conjunction with Fig. 1,2, illustrate whipping process：
Master control system 60 generates mixing control instruction when it is that reagent addition is completed to receive reagent status information；
For driving device 70 after receiving mixing control instruction, control agitating device 40 turns to target liquid filling hole with to mesh
Mixed liquor in mark reaction cup is stirred.
In the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention four provides, institute
State the stirring information that sensing state 50 is additionally operable to detection agitating device 40；The master control system 60, for receiving
After the completion of being stirring to stirring information, stirring reset instruction is generated；Driving device is connect with agitating device 40, and control is stirred
Mix the reset of device 40.
In conjunction with Fig. 1,2, illustrate the rotation process of storing apparatus 10：
Master control system 60 receive rotation predetermined angular number be pre-determined number when reaction rotary state information when,
Generate reagent rotation command；For storing apparatus 10 after receiving reagent rotation command, control storing apparatus 10 rotates predetermined angular,
Make next goal response cup alignment target liquid filling hole.
In the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention five provides, also
Including：Cleaning device 90, for the liquid in cleaning solution to be inputted the corresponding goal response cup of the target liquid filling hole with to institute
Goal response cup is stated to be cleaned.
As shown in Fig. 2, cleaning device 90 includes：Hydraulic device 91 enters goal response for controlling liquid in cleaning solution
Goal response cup is discharged in waste liquid in goal response cup by cup；Cleaning assembly 92 is connect with the hydraulic device 91, described
Under the action of hydraulic device 91, goal response cup is cleaned.
In the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention six provides, also
Including：
Client 110 is connect with the master control system 60, for providing user operation instruction input interface, and for adopting
The clear instruction that collection user inputs in the user operation instruction input interface, the master control system is sent to by the clear instruction
System 60；The driving device 70 controls the cleaning device 90 and is corresponded to target liquid filling hole after receiving the clear instruction
Goal response cup cleaned.The client 110 includes but not limited to host computer.
In the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention six provides, also
Including：
Temperature control device 80 adjusts the experimental temperature of reaction unit 20, makes experiment temperature under the driving effect of driving device 70
Degree is maintained at predetermined experimental temperature range.
In conjunction with Fig. 1,2, illustrate temperature adjustment process：
The sensing state 50 is additionally operable to the experimental temperature data of acquisition reaction unit 20.
The master control system 60 is worked as after receiving experimental temperature data, parsing the experimental temperature data
Preceding experimental temperature judges whether the current experiment temperature is more than the predetermined experimental temperature range, if so, generating temperature reduces
Control instruction or temperature increase control instruction.
Driving device 70 is after receiving temperature and reducing control instruction or temperature raising control instruction, control temperature device drop
Low or raising so that current experiment temperature is maintained at predetermined experimental temperature range.
In the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention seven provides, also wrap
It includes：Light-source system 90, for providing predetermined experiment illumination condition for the reaction cup in the reaction unit 20.The present invention can
To use existing Illumination adjusting technology, details are not described herein again.
In the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention eight provides, also wrap
It includes：Power-supply system 100 is connect with the master control system 60, the driving device 70, the light-source system 90, is the master control
System 60, the driving device 70, the light-source system 90 are powered.
The power-supply system 100 includes：First power subsystem 101, second source subsystem 102, third power supply subsystem
The 103, the 4th power subsystem 104 of system.
First power subsystem 101 is connected with the master control system 60, the driving device 70, is the master control system
60, the driving device 70 is powered；Second source subsystem 102 is connected with the driving device 70, is the driving device 70
Power supply；Third power subsystem 103 is connected with the driving device 70 and the temperature control device 80, is the driving device 70
It powers with the temperature control device 80；4th power subsystem 104 is connected with the light-source system 90, is the light-source system 90
Power supply.Wherein, first power subsystem 101 is 5V direct currents, and the second source subsystem 102 is 24V direct currents, institute
State third source subsystem 33, the 4th power subsystem 104 is 12V DC electricity；First power subsystem 101, second source
It is in parallel between subsystem 102, third power subsystem 103 and the 4th power subsystem 104.
In the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention nine provides, also wrap
It includes：Filter 4 is electrically connected with the master control system 60, is filtered for the alternating current in the preset range to input, will be filtered
Alternating current after wave is sent to the power-supply system 100.Wherein, the alternating current in the preset range is 120V-250V.
Fig. 3 is the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention ten provides
The flow diagram of operating method.
As shown in figure 3, a kind of operating method of biochemistry detection equipment for thioredoxin reductase Activity determination, packet
It includes：
Step S110, when sensing state detects that destination agent and/or target sample turn to mesh in storing apparatus
When marking thieff hatch and when reagent status information is that reagent adds completion status, reagent acquisition instructions are generated；
Step S120, driving device turn to apparatus for placing based on the reagent acquisition instructions received, control sampling apparatus
Destination sample hole at acquisition destination agent and/or target sample.
Step S130, when sensing state detects in reaction unit that goal response cup turns to target liquid filling hole and adopts
When the reagent status information of sampling device is that reagent acquires completion status, reagent addition instruction is generated；
Step S140, driving device add instruction based on the reagent received, and control sampling apparatus turns to reaction unit
Target liquid filling hole and destination agent and/or target sample are added in the corresponding goal response cup of target liquid filling hole.
Here, step S110 and step S120 is reagent adding procedure.Step S130 and step S140 is that reagent acquired
Journey.Under normal conditions, it is that first acquisition reagent adds reagent again, therefore step S120 will be prior to step S140.
It is that reagent acquisition is first, reagent is added rear in the entire experiment detection process of biochemistry detection equipment.
When primary acquisition, the signal of triggering reagent acquisition is only that destination agent and/or target sample turn to destination sample hole
Signal.When acquiring again later, the signal of triggering reagent acquisition is not only that destination agent and/or target sample turn to target
The signal of thieff hatch further includes reagent addition completion status signal.
Fig. 4 is the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention 11 provides
Operating method flow diagram.
As shown in figure 4, on the basis of embodiment ten, what the embodiment of the present invention 11 provided restores for thioredoxin
Enzyme (TR) detection biochemistry detection equipment operating method, further include：
Step S150, when sensing state detects that the reagent status information of sampling apparatus is that reagent addition is completed,
Generate stirring instruction；
Step S160, driving device based on receive stirring instruction, control agitating device turn to target liquid filling hole with
Mixed liquor in goal response cup is stirred.
Fig. 5 is the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention 12 provides
Operating method flow diagram.
As shown in figure 5, on the basis of embodiment 11, what the embodiment of the present invention 11 provided is used for thioredoxin also
Protoenzyme (TR) detection biochemistry detection equipment operating method, further include：
Step S170 is generated when sensing state detects that the stirring information of agitating device is that stirring is completed
Stir reset instruction；
Step S180, driving device are resetted based on the stirring reset instruction received, control agitating device.
Fig. 6 is the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention 13 provides
Operating method flow diagram.
As shown in fig. 6, the biochemical inspection for thioredoxin reductase (TR) detection that the embodiment of the present invention 13 provides
The operating method of measurement equipment further includes：
Step S210, when sensing state detects that the number that reaction unit rotates predetermined angular is pre-determined number,
Generate reagent rotation command；
Step S220, driving device are rotated predetermined angular, are made based on the reagent rotation command received, control storing apparatus
Obtain next goal response cup alignment target liquid filling hole.
In the biochemistry detection equipment for thioredoxin reductase (TR) detection that the embodiment of the present invention 14 provides
In operating method, further include：
When driving device receives the clear instruction that user is exported by user operation instruction input interface, based on reception
The clear instruction arrived controls the cleaning device and is cleaned to the corresponding goal response cup of target liquid filling hole.
Illustrate the operation principle of the present invention with reference to the detection process of thioredoxin reductase Activity determination in people's blood：
In the present invention, the quantity of reaction cup is 81 in reaction tray, is divided into control group and experimental group.There are two types of distribution sides
Formula, the first situation：The quantity of the reaction cup of control group is 40, and the quantity of the reaction cup of experimental group is then 41；Second
Situation：The quantity of the reaction cup of control group is 41, and the quantity of the reaction cup of experimental group is 40.81 reaction cups are divided into 9
Group, every group of reaction cup include 9 reaction cups.
Accommodating on disk, there are three circles to accommodate fixing piece, and it is 40 that often circle, which accommodates fixing piece quantity,.Wherein, first lap accommodates solid
Determine part and accommodates fixing piece for containing for holding specimen bottle, third circle for holding the first reagent bottle, the second circle receiving fixing piece
Put the second reagent bottle.
The final purpose of the present invention is that the first reagent, sample and the second reagent will be added in 81 reaction cups.It needs
Bright, involved the first reagent, the second reagent and sample are and thioredoxin reductase in the embodiment of the present invention
The relevant reagent of Activity determination.Wherein, sample is blood, body fluid or tissue homogenate.First reagent includes working solution
(TrisHCL, morpholinepropanesulfonic acid, disodium hydrogen phosphate citric acid buffer system and disodium hydrogen phosphate potassium dihydrogen phosphate buffer solution
Mixed solution) and thioredoxin reductase inhibiter compound.Second reagent is mix reagent, including 5,5- bis- is thio
Bis- (2- nitrobenzoic acids) or substitution 6,6'- dinitro -3,3'- dithiobenzoic acids and nicotinamide-adenine dinucleotide phosphate
Mixed solution.
Wherein, working solution configuration process is：According to 1:1:2:4 ratio takes reagent Tri(Hydroxymethyl) Amino Methane Hydrochloride
TrisHCL (0.025-0.125mol/L, PH 5.5.8-7.2), morpholinepropanesulfonic acid (0.25mol/L), disodium hydrogen phosphate lemon
Acid buffering system 0.2mol/L and disodium hydrogen phosphate potassium dihydrogen phosphate buffer solution 1-15mol/L；Wherein, the phosphoric acid hydrogen two
The PH of sodium citric acid buffer system is 2.2-8.0；The PH of the disodium hydrogen phosphate potassium dihydrogen phosphate buffer solution is 4.9-8.2；
Then by the TrisHCL (Tri(Hydroxymethyl) Amino Methane Hydrochloride), morpholinepropanesulfonic acid, disodium hydrogen phosphate citric acid buffer body
System and disodium hydrogen phosphate potassium dihydrogen phosphate buffer solution are uniformly mixed.Wherein, with 1:1-1:5 ratio mix the working solution and
Inhibitor forms the inhibitor solution；The inhibitor solution is uniformly mixed；The wherein described inhibitor is thioredoxin
Reductase inhibiter compounds.It can be chemical monomer selenium quinoline class compound.With 1:2-1:8 ratio mix the reagent A and
The reagent B, forms the mix reagent；Specifically, reagent A and the mixed proportion of reagent B ranging from 1:2-1:8, preferably
With 1:The ratio of 4-5 comes mix reagent A and reagent B, and it is higher to mix the accuracy of the mix reagent of generation when detecting at this time,
In, the A reagents are 5,5-Dithiobis (2-nitrobenzoic acid) or substitution 6,6'- dinitro -3,3'- dithiobenzoic acids；B
Reagent is nicotinamide-adenine dinucleotide phosphate.The part can be found in another application for a patent for invention " one of same applicant
Kind human peripheral thioredoxin reductase activity test method ".
About the setting quantity for adopting fluid apertures, when only needing to acquire a kind of reagent, then only need to be arranged on accommodating disk cover
One is adopted fluid apertures；When needing to acquire plurality of reagents, then need multiple to adopt fluid apertures accommodating disk cover setting so that each reagent pair
A liquid filling hole, the purpose being arranged in this way is answered to be to speed up fluid production rate.Thioredoxin reductase is lived in people's blood of the present invention
Property test experience in, due to needing to acquire three kinds of reagents, the present invention is provided with three and adopts fluid apertures, the first reagent correspondence the
One adopts fluid apertures, and sample corresponds to second and adopts fluid apertures, and the second reagent corresponds to third and adopts fluid apertures.
About the setting quantity of liquid filling hole, the application is provided with two liquid filling holes (first to improve the speed of liquid feeding
Liquid filling hole and the second liquid filling hole).In period 1 group, only add the first reagent to first group of reaction cup, can add by first
Fluid apertures or the second liquid filling hole add the first reagent.When second round group, originally to first group of reaction cup sample-adding, second group of reaction cup is given
Add the first reagent, the first reagent and sample can be added by the first liquid filling hole and the second liquid filling hole.When period 3 group, to the
One group of reaction cup adds the second reagent, originally to second group of reaction cup sample-adding, adds the first reagent to third group reaction cup, later, according to
The liquid feeding sequence of third group continues that the first reagent, sample and the second reagent is added to second group of reaction cup to the 9th group of reaction cup.
Illustrate the experimentation of present invention detection thioredoxin reductase with reference to Fig. 7：
S1, the period 1 of period 1 group, driving device control accommodate disk rotation, when condition checkout gear 50 is examined
It measures when the first reagent in storing apparatus turns to the first liquid filling hole or the second liquid filling hole and reagent status information is added for reagent
When completion status, the first reagent acquisition instructions are generated；
S2, driving device turn to apparatus for placing based on the first reagent acquisition instructions received, control sampling apparatus
First thieff hatch acquires the first reagent；
S3, when condition checkout gear detect first reaction cup in first group of reaction cup turn to the first liquid filling hole or
It is raw when the second liquid filling hole and when sensing state 50 detects that the reagent status information of sampling apparatus 30 is that reagent acquisition is completed
It adds and instructs at the first reagent；
S4, for driving device 70 after receiving the addition instruction of the first reagent, control sampling apparatus 30 turns to reaction unit
First reagent is simultaneously added to the first liquid filling hole or the second liquid filling hole corresponding first by 20 the first liquid filling hole or the second liquid filling hole
In a reaction cup.
S5, after first reaction cup in first group of reaction cup has added the first reagent, by first group of reaction cup
Two reaction cups turn to the first liquid filling hole or the second liquid filling hole, continue to execute the first reagent of the first reagent of above-mentioned acquisition and addition
Process, until by first group of reaction cup 9 reaction cups all added the first reagent, period 1 group terminates.
S6, the period 1 of second round group, driving device control reaction unit 20 and rotate, and work as sensing state
The examination of sampling apparatus when 50 first reaction cup for detecting in reaction unit in second group of reaction cup turn to the first liquid filling hole
When agent status information is that reagent acquires completion status, the addition instruction of the first reagent is generated.
S7, driving device add instruction based on the reagent received, and control sampling apparatus turns to the first of reaction unit
First reagent is simultaneously added in corresponding first reaction cup of the first liquid filling hole by liquid filling hole；
S8, when sensing state detects that the reagent status information of sampling apparatus in reaction unit is addition completion status
When, generate the first reagent acquisition instructions；
S9, driving device turn to storing apparatus based on the first reagent acquisition instructions received, control sampling apparatus
First thieff hatch acquires the first reagent；
S10, when condition checkout gear detects that first reaction cup in second group of reaction cup turns to the first liquid filling hole
Or when the second liquid filling hole and when sensing state 50 detects that the reagent status information of sampling apparatus 30 is that reagent acquisition is completed,
Generate the addition instruction of the first reagent；
S11, for driving device 70 after receiving the addition instruction of the first reagent, control sampling apparatus 30 turns to reaction unit
First reagent is simultaneously added in corresponding first reaction cup of the first liquid filling hole by 20 the first liquid filling hole.
Sampling apparatus according to the first reagent of above-mentioned acquisition process collecting sample, according to a cycle of second round group
The sequence for adding the first reagent, second period addition sample, until the first examination is added in 9 reaction cups in second group of reaction cup
Sample is added in 9 reaction cups in agent and first group of reaction cup, and second round group terminates.
In a cycle of period 3 group, the first reagent is added for third group reaction cup；Second period is second
Group reaction cup adds sample；The third period is that first group of reaction cup adds the second reagent, until will be in third group reaction cup
The first reagent is added in 9 reaction cups, 9 reaction cups addition sample reagent in second group of reaction cup, and 9 in first group of reaction cup
The second reagent is added in a reaction cup, and period 3 group terminates.
According to the liquid feeding sequence of period 3 group, the first reagent, sample and third examination will be added in 4-9 group reaction cups
Agent, until detection is completed or stopped in the detection of whole disk.Another application for a patent for invention that the part can be found in same applicant is " a kind of
The active cooperation detection device and method of thioredoxin reductase ".
It should be noted that after waiting for that each group of reaction cup all adds the first reagent, sample and the second reagent, that is, start
It reacts, reaction time up to 22 periods (each period 22.5s), it is preferable that be 20 periods, to this group of reaction cup
For, within this 22 periods, if this group of reaction cup turns to liquid feeding position, liquid-transfering device standing is waited for.It waits for
This group of reaction cup after reaction, can send instruction control hydraulic device, and then control cleaning device will be reacted by client
Waste liquid after being reacted in cup is taken away, and is cleaned to reaction cup, this group of reaction cup can proceed with addition after the completion of cleaning
The operation of first reagent, sample and the second reagent.
Such as：For first group of reaction cup, after period 3 group, in first group of reaction cup includes the first examination
Reaction takes place in the mixed liquor of agent, sample and the second reagent, at this point, in next cycle, if first group of reaction tray
Reaction cup turns to liquid feeding position, then liquid-transfering device standing is waited for.
It, can also be to non-biochemical reaction since the biochemistry detection equipment of the present invention can not only be detected biochemical reaction
It is detected, therefore it may only be necessary to the biochemical reaction parameter done required for the user input interface input of client or non-biochemistry
Response parameter can be automatically adjusted according to the detection process of specific biochemical reaction or non-biochemical reaction.
The present invention is directed to protect a kind of biochemistry detection equipment in people's blood for thioredoxin reductase biochemistry detection and
Its operating method, on the one hand, biochemistry detection equipment of the invention realizes thioredoxin reductase activity in people's blood and examines for the first time
The full-automatic detection of survey solves intelligent by having been manually done asking for thioredoxin reductase Activity determination in the prior art
Topic；On the other hand, the biochemistry detection equipment of the application can not only realize single sample the whole series TR testing processes, additionally it is possible to realize
Multiple samples are continuously finished TR testing processes.
Specifically, 1) by the configuration of hardware setting arrangement, including reaction tray, the configuration of disk, the connection between two disks are accommodated
Flowing mode, sample-adding and sampling apparatus etc., may be implemented the completion of the whole detection flow for single sample.Due to for sample
Continuous processing, continuous sample-adding and reaction time require stringent, and since sample disk often rotates two lattice, reaction tray rotates a lattice, i.e.,
Each sample tube takes 2 samples, is separately added into two reaction cups, as the detection of experimental group and control group data, therefore can
Complete the synchronous detection of single sample.
2) pass through the improvement of hardware setting arrangement and method, wherein hardware setting includes the configuration of reaction tray, sample disk
It configures, the linked manner between two disks, the completion of the continuous testing process of multisample may be implemented in sample-adding and sampling apparatus etc..Side
The improvement of method includes the rotation time for sample disk and reaction tray defined, and rotation interval, rotation distance, sampling order adds
The schedulings such as sample time, since each group of reaction cup needs to add solution three times, thus can be in each group of reaction cup solubilization liquid
During start simultaneously at the liquid feeding of next group of reaction cup, in such manner, it is possible to so that multiple samples are carried out continuously detection, and can follow
Ring detects, so as to shorten detection time.
In addition, the present invention uses specific driving hardware, software and hardware is enable to cooperate, clinical TR can be improved
Detection efficiency saves cost.And the experimental result that biochemistry detection equipment through the invention detects, it disclosure satisfy that national detection
Standard requirement.
It should be understood that the above-mentioned specific implementation mode of the present invention is used only for exemplary illustration or explains the present invention's
Principle, but not to limit the present invention.Therefore, that is done without departing from the spirit and scope of the present invention is any
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.In addition, appended claims purport of the present invention
Covering the whole variations fallen into attached claim scope and boundary or this range and the equivalent form on boundary and is repairing
Change example.
Claims (19)
1. a kind of biochemistry detection equipment for thioredoxin reductase Activity determination, which is characterized in that including：
Storing apparatus (10) for holding multiple reagents and/or sample, and under the driving effect of driving device (70), surrounds
Axle center periodic rotary makes destination agent and/or target sample turn to target liquid filling hole；
Reaction unit (20) surrounds the axle center period for holding multiple reaction cups, and under the driving effect of driving device (70)
Property rotation so that goal response cup turn to destination sample hole；
Sampling apparatus (30), under the driving effect of driving device (70), surrounding axle center periodic rotary, will be adopted from target
The destination agent and/or target sample acquired in sample hole is added in the corresponding goal response cup of target liquid filling hole；Shape
State sensing device (50), the receiving of reaction rotary state information, storing apparatus (10) for detecting the reaction unit (20)
The reagent status information of rotary state information, sampling apparatus (30)；
Master control system (60) is connect with the sensing state (50), driving device (70) respectively, for based on reaction rotation
Status information accommodates rotary state information and reagent status information, generates and refers to the corresponding control of driving device (70) transmission
It enables；
Driving device (70), respectively even with the sampling apparatus (30), the storing apparatus (10), the reaction unit (20)
It connects, for based on the control instruction received, controlling the sampling apparatus (30), the reaction unit (20), receiving dress
It sets (10) and executes corresponding operation.
2. biochemistry detection equipment according to claim 1, which is characterized in that the storing apparatus (10) includes：
Disk (11) is accommodated, activity setting does periodic rotary around axle center；
Multiple receiving fixing pieces (12) are used for containing reagent bottle and/or specimen bottle.
3. biochemistry detection equipment according to claim 1, which is characterized in that the reaction unit (20) includes：
Reaction tray (21), activity setting, periodic rotary is done around axle center；
Multiple reaction cup fixing pieces (22), for holding reaction cup.
4. biochemistry detection equipment according to claim 1, which is characterized in that the sampling apparatus (30) includes：
Sample revolving part (31), activity setting, under the driving effect of driving device (70), periodically turning around axle center
It is dynamic；
Fixing piece (32) is sampled, is fixed on sampled rotational part (31), is turned under the induced effect of sampling revolving part (32)
It is dynamic；
Sampling needle (33), one end are fixing end, and the other end is free end.
5. biochemistry detection equipment according to claim 1, which is characterized in that
Reaction rotary state information of the master control system (60) when receiving goal response cup and turning to target liquid filling hole and
When reagent status information is that reagent acquires completion status, reagent addition instruction is generated；
For the driving device (70) after receiving reagent addition instruction, control sampling apparatus (30) turns to reaction unit (20)
Target liquid filling hole and destination agent and/or target sample are added in the corresponding goal response cup of target liquid filling hole.
6. biochemistry detection equipment according to claim 1, which is characterized in that
Receiving of the master control system (60) when receiving destination agent and/or whether target sample turns to destination sample hole
When rotary state information and when reagent status information is that reagent adds completion status, reagent acquisition instructions are generated；
For the driving device (70) after receiving reagent acquisition instructions, control sampling apparatus (30) turns to apparatus for placing (10)
Destination sample hole at acquisition destination agent and/or target sample.
7. biochemistry detection equipment according to claim 1, which is characterized in that the biochemistry detection equipment further includes：
Agitating device (40), under the driving effect of driving device (70), periodic rotary to be done with to target around axle center
The mixed liquor formed in reaction cup is stirred.
8. biochemistry detection equipment according to claim 7, which is characterized in that the agitating device (40) includes：
Stir revolving part (41), activity setting, under the driving effect of driving device (70), periodically turning around axle center
It is dynamic；
Fixing piece (42) is stirred, is fixed on stirring rotating member (31), is turned under the induced effect of stirring revolving part (42)
It is dynamic；
Mixing needle (43), one end are fixing end, and the other end is free end.
9. biochemistry detection equipment according to claim 7, which is characterized in that
The master control system (60) generates mixing control instruction when it is that reagent addition is completed to receive reagent status information；
For driving device (70) after receiving mixing control instruction, control agitating device (40) turns to target liquid filling hole with to mesh
Mixed liquor in mark reaction cup is stirred.
10. the biochemistry detection equipment according to any one of claim 7-9, which is characterized in that the sensing state
(50) it is additionally operable to the stirring information of detection agitating device (40)；
The master control system (60), for after the completion of it is stirring to receive stirring information, generating stirring reset instruction；
Driving device is connect with agitating device (40), and control agitating device (40) resets.
11. biochemistry detection equipment according to claim 1, which is characterized in that
Reaction rotary state information of the master control system (60) when receiving the number of rotation predetermined angular and being pre-determined number
When, generate reagent rotation command；
For storing apparatus (10) after receiving reagent rotation command, control storing apparatus (10) rotates predetermined angular so that next
A goal response cup alignment target liquid filling hole.
12. biochemistry detection equipment according to claim 1, which is characterized in that further include：
Cleaning device (90), for the liquid in cleaning solution to be inputted the corresponding goal response cup of the target liquid filling hole with to institute
Goal response cup is stated to be cleaned.
13. biochemistry detection equipment according to claim 12, which is characterized in that the cleaning device (90) includes：
Hydraulic device (91), for control liquid in cleaning solution enter goal response cup or by goal response cup waste liquid be discharged
Goal response cup；
Cleaning assembly (92) is connect with the hydraulic device (91), under the action of the hydraulic device (91), to goal response
Cup is cleaned.
14. biochemistry detection equipment according to claim 12 or 13, which is characterized in that further include：
Client (110) is connect with the master control system (60), for providing user operation instruction input interface, and for adopting
The clear instruction that collection user inputs in the user operation instruction input interface, the master control system is sent to by the clear instruction
It unites (60)；
The driving device (70) controls the cleaning device (90) to target liquid filling hole pair after receiving the clear instruction
The goal response cup answered is cleaned.
15. a kind of operating method of biochemistry detection equipment for thioredoxin reductase Activity determination, which is characterized in that packet
It includes：
When sensing state detects that goal response cup in reaction unit turns to the reagent of target liquid filling hole and sampling apparatus
When status information is that reagent acquires completion status, reagent addition instruction is generated；
Driving device adds instruction based on the reagent received, and control sampling apparatus turns to the target liquid filling hole of reaction unit simultaneously
Destination agent and/or target sample are added in the corresponding goal response cup of target liquid filling hole；
When sensing state detects that destination agent and/or target sample turn to destination sample hole in storing apparatus and examination
When agent status information is that reagent adds completion status, reagent acquisition instructions are generated；
Driving device is turned to based on the reagent acquisition instructions received, control sampling apparatus at the destination sample hole of apparatus for placing
Acquire destination agent and/or target sample.
16. operating method according to claim 15, which is characterized in that further include：
When sensing state detects that the reagent status information of sampling apparatus is that reagent addition is completed, stirring instruction is generated；
Driving device turns to target liquid filling hole in goal response cup based on the stirring instruction received, control agitating device
Mixed liquor be stirred.
17. operating method according to claim 16, which is characterized in that further include：
When sensing state detects that the stirring information of agitating device is that stirring is completed, stirring reset instruction is generated；
Driving device is resetted based on the stirring reset instruction received, control agitating device.
18. according to the operating method described in any one of claim 16-17, which is characterized in that further include：
When sensing state detects that the number that reaction unit rotates predetermined angular is pre-determined number, generates reagent rotation and refer to
It enables；
Driving device rotates predetermined angular based on the reagent rotation command received, control storing apparatus so that next target
Reaction cup alignment target liquid filling hole.
19. according to the operating method described in any one of claim 16-17, which is characterized in that further include：
When driving device receives the clear instruction that user is exported by user operation instruction input interface, based on what is received
The clear instruction controls the cleaning device and is cleaned to the corresponding goal response cup of target liquid filling hole.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710170517.9A CN108627659A (en) | 2017-03-21 | 2017-03-21 | The biochemistry detection equipment and its operating method of thioredoxin reductase detection |
| PCT/CN2018/079787 WO2018171619A1 (en) | 2017-03-21 | 2018-03-21 | Method for detecting activity of thioredoxin reductase, detection device and operation method therefor |
| US16/496,207 US20200190556A1 (en) | 2017-03-21 | 2018-03-21 | Method for detecting activity of thioredoxin reductase, detection device and operation method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710170517.9A CN108627659A (en) | 2017-03-21 | 2017-03-21 | The biochemistry detection equipment and its operating method of thioredoxin reductase detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108627659A true CN108627659A (en) | 2018-10-09 |
Family
ID=63687247
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710170517.9A Pending CN108627659A (en) | 2017-03-21 | 2017-03-21 | The biochemistry detection equipment and its operating method of thioredoxin reductase detection |
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|---|---|
| CN (1) | CN108627659A (en) |
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| CN101578522A (en) * | 2007-01-09 | 2009-11-11 | 奥林巴斯株式会社 | Analyzer |
| CN101865927A (en) * | 2009-04-17 | 2010-10-20 | 武汉医尔特科技有限公司 | Novel full-automatic blood type analyzer |
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|---|---|---|---|---|
| JPH10132735A (en) * | 1996-10-28 | 1998-05-22 | Hitachi Ltd | Automatic analyzing device |
| CN1963527A (en) * | 2005-11-10 | 2007-05-16 | 深圳迈瑞生物医疗电子股份有限公司 | Full-automatic biochemical analyzer and analysis method thereof |
| CN101578522A (en) * | 2007-01-09 | 2009-11-11 | 奥林巴斯株式会社 | Analyzer |
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