CN108624534A - Method for discovering novel antibiotic actinomycetes and antibacterial and antiviral product from natural product - Google Patents
Method for discovering novel antibiotic actinomycetes and antibacterial and antiviral product from natural product Download PDFInfo
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- CN108624534A CN108624534A CN201810472008.6A CN201810472008A CN108624534A CN 108624534 A CN108624534 A CN 108624534A CN 201810472008 A CN201810472008 A CN 201810472008A CN 108624534 A CN108624534 A CN 108624534A
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- albidus
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 18
- 230000003115 biocidal effect Effects 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 11
- 230000000840 anti-viral effect Effects 0.000 title abstract description 4
- 241000186361 Actinobacteria <class> Species 0.000 title abstract 3
- 229930014626 natural product Natural products 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 claims abstract description 20
- 239000010802 sludge Substances 0.000 claims abstract description 11
- 238000000855 fermentation Methods 0.000 claims abstract description 10
- 230000004151 fermentation Effects 0.000 claims abstract description 10
- 239000000725 suspension Substances 0.000 claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 8
- 239000003443 antiviral agent Substances 0.000 claims abstract description 6
- 239000012535 impurity Substances 0.000 claims abstract description 5
- 239000003814 drug Substances 0.000 claims abstract description 4
- 235000013373 food additive Nutrition 0.000 claims abstract description 4
- 239000002778 food additive Substances 0.000 claims abstract description 4
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 241000186046 Actinomyces Species 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 10
- 230000002155 anti-virotic effect Effects 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 241001465754 Metazoa Species 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 4
- 230000009514 concussion Effects 0.000 claims description 3
- 239000013078 crystal Substances 0.000 claims description 3
- 238000003113 dilution method Methods 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
- 239000003960 organic solvent Substances 0.000 claims description 3
- 230000035755 proliferation Effects 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 abstract description 2
- 231100001083 no cytotoxicity Toxicity 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- 239000003674 animal food additive Substances 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000010790 dilution Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 abstract 1
- 239000005445 natural material Substances 0.000 abstract 1
- 241001446247 uncultured actinomycete Species 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 241000958272 Streptomyces albidus Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 208000030507 AIDS Diseases 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 description 1
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 1
- 208000005577 Gastroenteritis Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 208000009877 Pyelocystitis Diseases 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 206010009887 colitis Diseases 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for discovering a novel antibiotic actinomycete and an antibacterial and antiviral product from natural substances, which comprises the following steps: removing impurities from the seabed sludge, weathering the sludge from which the impurities are removed, preparing sludge suspension by adopting a dilution separation method, shaking uniformly, sucking the sludge suspension, placing the sludge suspension on a flat plate for culturing, and selecting a part containing Streptomyces-albicus-55 bacterial strains; putting the strain into a culture bottle, adding a culture medium for enrichment culture, selecting mature strains, and putting the strains into the culture medium for slant culture; after slant culture, the dominant strain is placed in a fermentation tank for fermentation culture, and the fermentation liquor is filtered and concentrated to separate and prepare the antibacterial and antiviral agent, the veterinary medicine, the food additive and the feed additive. The novel antibiotic actinomycetes produced by the method has low cost, and the prepared novel antibiotic actinomycetes has no cytotoxicity and no cell deformation, and is applied to the technical field of preparation of various antibacterial and antiviral products.
Description
Technical field
The present invention relates to the methods for finding new antibiotic actinomyces and anti-bacteria and anti-virus product from natural goods, belong to biology
Product technical field.
Background technology
ALBIJJIN is the antibiotic newly isolated from natural goods, and production bacterial strain is Streptomyces-albidus-
55 produce natural antibiont ALBIJJIN in the zymotic fluid of bacterial strain Streptomyces-albidus-55.
ALBIJJIN acts on the cell membrane of bacterium, destroys the metabolic process of bacterium, has antibacterial action for various bacteriums,
Especially to patience staphylococcus, patience coliform plays the role of specificity.
The immune substances such as the antibody such as interferon and cytohormone of specificity can be generated after ALBIJJIN uses in the body,
There is apparent antivirus action for avian influenza virus and various virus spreads and foot and mouth disease.
ALBIJJIN acts on aphid and the digestive system of other insects, causes vomiting or diarrhea, leads to the de- of severe
Water and malnutrition have pest-resistant effect, can be applicable to the pest-resistant preparation of fruit and vegetables.
ALBIJJIN is cell free viral, and acellular deformation does not have hereditary variation after use, is safety.
The production cost of ALBIJJIN is low, is not influenced on ecological environment, be production and application and its rational product, according to
Understand, proposes that the antibiotics as ALBIJJIN, similar product do not have currently without people yet.
The shortcomings that antiviral agents such as existing antibiotic, streptomysin, toximycin, is antibacterial and antiviral is limited in scope
System plays the role of specificity, using leaving toxicity and hereditary variation, side effect in rear body for one or two kind of bacterium or virus
Greatly.
Invention content
In view of the above-mentioned problems, the technical problem to be solved in the present invention is to provide find new antibiotic actinomyces from natural goods
And the method for anti-bacteria and anti-virus product.
The method that the slave natural goods of the present invention find new antibiotic actinomyces and anti-bacteria and anti-virus product includes following step
Suddenly：
Step 1: impurity will be removed in bottom silt first, shell is removed, fishbone and other nautical miles of animals and plants torture object, so
Deimpurity mud will be gone to carry out weathering under certain temperature and humidity environment afterwards, weathering time is according to the different time of material
Also it has any different；
Step 2: the mud after selective weathering is put into sterile water vial, sludge suspension liquid is prepared using isolation by dilution method,
Sludge suspension liquid is drawn after shaking up to be placed on tablet and is cultivated, then by micro- sem observation select containing
Streptomyces-albidus-55 bacterial strains part；
Proliferation training is carried out Step 3: will be put into containing Streptomyces-albidus-55 bacterial strains in culture bottle and culture medium is added
It supports, cultivates 6-10 days, the bacterial strain for picking out maturation is put into culture medium and carries out inclined-plane culture；
It handles to obtain a large amount of dominant bacteria Step 4: carrying out concussion during inclined-plane culture, then again places dominant bacteria
Fermented and cultured is carried out in fermentation tank, is concentrated after fermentation culture is filtered, and anti-bacteria and anti-virus agent, animal doctor are prepared in separation
Drug, food additives and feed addictive.
Further preferably, Streptomyces-albidus-55 bacterial strains are actinomyces, fermented and cultured in the step four
Liquid is ALBIJJIN, is in shallow maroon, pure aroma taste, ph=4, the white crystals detached in zymotic fluid have water solubility, all
Organic solvent in will not dissolve, fusing point is 865 DEG C, and heat resistance is strong, total protein concentration be trap identify OD28Index is 2.0, is had
Largely necessary amino acid forms complicated structure with trace element, and molecular weight is big.
Beneficial effects of the present invention：The new antibiotic actinomyces for adopting this method production are at low cost, to week when production
The environment enclosed does not influence, and the new antibiotic actinomyces no cytotoxicity prepared, acellular deformation, and can be applied to a variety of
The preparing technical field of anti-bacteria and anti-virus product.
Description of the drawings：
Fig. 1 is the treptomyces-albidus-55 bacterial strains detached in the specific embodiment of the invention and standard
Streptomyces-albidus bacterial strain antibiotic property test structure analysis charts.
Specific implementation mode：
Present embodiment is described in further detail invention using following technical scheme and embodiment.
The method that the slave natural goods of the present invention find new antibiotic actinomyces and anti-bacteria and anti-virus product includes following step
Suddenly：
Step 1: impurity will be removed in bottom silt first, shell is removed, fishbone and other nautical miles of animals and plants torture object, so
Deimpurity mud will be gone to carry out weathering under certain temperature and humidity environment afterwards, weathering time is according to the different time of material
Also it has any different；
Step 2: the mud after selective weathering is put into sterile water vial, sludge suspension liquid is prepared using isolation by dilution method,
Sludge suspension liquid is drawn after shaking up to be placed on tablet and is cultivated, then by micro- sem observation select containing
Streptomyces-albidus-55 bacterial strains part；
Proliferation training is carried out Step 3: will be put into containing Streptomyces-albidus-55 bacterial strains in culture bottle and culture medium is added
It supports, cultivates 6-10 days, the bacterial strain for picking out maturation is put into culture medium and carries out inclined-plane culture；
It handles to obtain a large amount of dominant bacteria Step 4: carrying out concussion during inclined-plane culture, then again places dominant bacteria
Fermented and cultured is carried out in fermentation tank, is concentrated after fermentation culture is filtered, and anti-bacteria and anti-virus agent, animal doctor are prepared in separation
Drug, food additives and feed addictive.
Further preferably, Streptomyces-albidus-55 bacterial strains are actinomyces, fermented and cultured in the step four
Liquid is ALBIJJIN, is in shallow maroon, pure aroma taste, ph=4, the white crystals detached in zymotic fluid have water solubility, all
Organic solvent in will not dissolve, fusing point is 865 DEG C, and heat resistance is strong, total protein concentration be trap identify OD28Index is 2.0, is had
Largely necessary amino acid forms complicated structure with trace element, and molecular weight is big.
The Streptomyces-albidus of treptomyces-albidus-55 bacterial strains and standard will be detached in step 4
Bacterial strain carries out antibiotic property experiment, from the test result of Fig. 1 it is found that the treptomyces-albidus-55 bacterial strains isolated have
Preferable antibiotic property.
Anti-bacteria and anti-virus agent prepared by step 4 is applied to AIDS, colitis, and pyelocystitis, Poisoning digestion are not
Good, the therapeutic effect highly significant in the fields such as gastroenteritis also has the effect of relatively good for animals and plants epidemic prevention.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (2)
1. the method for finding new antibiotic actinomyces and anti-bacteria and anti-virus product from natural goods, it is characterised in that：Specific side
Method is：Step 1: impurity will be removed in bottom silt first, shell is removed, fishbone and other nautical miles of animals and plants torture object, so
Deimpurity mud will be gone to carry out weathering under certain temperature and humidity environment afterwards, weathering time is according to the different time of material
Also it has any different；
Step 2: the mud after selective weathering is put into sterile water vial, sludge suspension liquid is prepared using isolation by dilution method,
Sludge suspension liquid is drawn after shaking up to be placed on tablet and is cultivated, then by micro- sem observation select containing
Streptomyces-albidus-55 bacterial strains part；
Proliferation training is carried out Step 3: will be put into containing Streptomyces-albidus-55 bacterial strains in culture bottle and culture medium is added
It supports, cultivates 6-10 days, the bacterial strain for picking out maturation is put into culture medium and carries out inclined-plane culture；
It handles to obtain a large amount of dominant bacteria Step 4: carrying out concussion during inclined-plane culture, then again places dominant bacteria
Fermented and cultured is carried out in fermentation tank, is concentrated after fermentation culture is filtered, and anti-bacteria and anti-virus agent, animal doctor are prepared in separation
Drug, food additives and feed addictive.
2. finding the ALBIJJIN of new antibiotic actinomyces and anti-bacteria and anti-virus product from natural goods, it is characterised in that：It is described
The step of four in Streptomyces-albidus-55 bacterial strains be actinomyces, fermentation culture is ALBIJJIN, be in shallow maroon,
Pure aroma taste, ph=4, the white crystals detached in zymotic fluid have water solubility, will not be dissolved in all organic solvents,
Fusing point is 865 DEG C, and heat resistance is strong, and total protein concentration is that trap identifies OD28Index is 2.0, have largely must amino acid with it is micro-
Secondary element forms complicated structure, and molecular weight is big.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810472008.6A CN108624534A (en) | 2018-05-17 | 2018-05-17 | Method for discovering novel antibiotic actinomycetes and antibacterial and antiviral product from natural product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810472008.6A CN108624534A (en) | 2018-05-17 | 2018-05-17 | Method for discovering novel antibiotic actinomycetes and antibacterial and antiviral product from natural product |
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|---|---|
| CN108624534A true CN108624534A (en) | 2018-10-09 |
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|---|---|---|---|
| CN201810472008.6A Pending CN108624534A (en) | 2018-05-17 | 2018-05-17 | Method for discovering novel antibiotic actinomycetes and antibacterial and antiviral product from natural product |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103966141A (en) * | 2014-05-20 | 2014-08-06 | 淮海工学院 | Method for rapidly screening antibacterial activity bacteria and actinomycetes |
| CN105349448A (en) * | 2015-09-09 | 2016-02-24 | 中国热带农业科学院热带生物技术研究所 | Marine actinomycete with antiviral activity and application of marine actinomycete with antiviral activity |
-
2018
- 2018-05-17 CN CN201810472008.6A patent/CN108624534A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103966141A (en) * | 2014-05-20 | 2014-08-06 | 淮海工学院 | Method for rapidly screening antibacterial activity bacteria and actinomycetes |
| CN105349448A (en) * | 2015-09-09 | 2016-02-24 | 中国热带农业科学院热带生物技术研究所 | Marine actinomycete with antiviral activity and application of marine actinomycete with antiviral activity |
Non-Patent Citations (1)
| Title |
|---|
| 马毅敏等: "海洋放线菌代谢产物蒽环类化合物研究进展", 《药物生物技术》 * |
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Application publication date: 20181009 |