CN108624496A - A kind of microorganism mutation breeding instrument sample transmission and collection device - Google Patents
A kind of microorganism mutation breeding instrument sample transmission and collection device Download PDFInfo
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- CN108624496A CN108624496A CN201710177112.8A CN201710177112A CN108624496A CN 108624496 A CN108624496 A CN 108624496A CN 201710177112 A CN201710177112 A CN 201710177112A CN 108624496 A CN108624496 A CN 108624496A
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- sample
- limiting plate
- hole
- collection device
- support plate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
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- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
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- Microbiology (AREA)
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- General Chemical & Material Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of microorganism mutation breeding instrument sample transmission for belonging to breeding instrument components range and collection devices, including sample limiting plate, sample support plate and EP pipe supports, wherein, sample support plate is close to below sample limiting plate, it is used to support sample limiting plate, EP pipe supports are located at below sample support plate, each frame position on the EP pipe supports is corresponded with the through-hole on sample limiting plate, drive translation stage that can realize moving horizontally for sample limiting plate and EP pipe supports using motor, through-hole through sample support plate left end after making the sample in through-hole be handled is fallen into corresponding EP pipes, realize the integration of sample treatment and collection.
Description
Technical field
The invention belongs to breeding instrument components range, more particularly to a kind of microorganism mutation breeding instrument is transmitted and is received with sample
Acquisition means.
Background technology
Microbial strains best in quality are the core of biological industry always, and efficient microorganism induced-mutation technique is then weight
In it is weight.The problems such as conventional microorganism mutation breeding method generally existing working efficiency is low, heavy workload, big blindness, changes
It learns mutagenesis also easily to cause actual bodily harm to operating personnel, pollute the environment, the mutagenesis performance of physical mutagenesis is poor and micro- life
Certain tolerance is produced after object Reusability, other methods are required to complicated professional equipment, with high costs.
Plasma technique not only overcomes the conventional breeding period as a kind of non-transgenic mutation breeding new technology
It is long, process is slow, the shortcomings of being difficult to obtain high-quality mutational variety, and have more efficiency and cost advantage than genetic engineering, especially
To the biological cell with complicated metabolism network, non-transgenic method of mutagenesis more has its unique advantage.
Current existing plasma mutation breeding instrument is often only capable of handling one or several samples.CN 202379975U
Disposable microorganism mutation breeding machine slide glass is disclosed, each slide glass unit is detachable, and mutagenesis operation can be made to be carried out continuously；
CN104017718B discloses disposable microorganism mutation breeding instrument load plate and carries bacterium device, without taking repeatedly in mutagenic processes
Put load plate.
However, above-mentioned apparatus is required for once placing the sample of required mutagenesis, it is pending in the case where sample is more
The time that sample waits in working bin will be longer, then before handling, the risk of biological sample microbiological contamination will be sharply increased.Into one
Step, if pending sample is fluid sample, after placing the long period in clean working bin, the liquid in biological sample
Ingredient, such as protective agent ingredient will evaporate, so as to cause when handling the sample it is possible that have enough
Sample size is handled, and the decline of sample treatment quality is even resulted in.On the other hand, if treated, sample cannot carry out in time
It collects, will also influence the effect of mutation breeding.
Invention content
It is existing to solve the purpose of the present invention is to provide a kind of microorganism mutation breeding instrument sample transmission and collection device
Have in technology and there are problems that only to realize sample transmission by two dimensional motion and collect.
Technical scheme is as follows：
A kind of microorganism mutation breeding instrument sample transmission and collection device, the sample transmission and collection device include sample limit
Position plate 1, sample support plate 2 and EP pipe supports 3, the EP pipe supports 3 are fixed on the tablet vertical with the sample limiting plate 1, institute
It states sample support plate 2 and is close to 1 lower section of sample limiting plate, the EP pipe supports 3 are located at 2 lower section of sample support plate, the sample limit
Be provided with equidistant through-hole on plate 1, the through-hole forms groove on sample support plate 2, the through-hole on EP pipe supports 3
EP pipes correspond, and there are one through-holes for the left end setting of the sample support plate 2, are moved to the left the sample limiting plate 1 and EP
Pipe support 3 makes the sample in groove reach specific position and is handled, and the sample after handling passes sequentially through sample support plate 2 again
The through-hole of left end is fallen into the EP pipes of EP pipe supports 3.
The sample support plate 2 is fixed on holder.Sample support plate 2 is fixed on the holder of vertical direction, and tight
Sample limiting plate 1 is pasted, so that the through-hole on sample limiting plate 1 is formed groove on sample support plate 2, for holding sample or sample
Slide glass.
The sample limiting plate 1 is connect with lifting platform 8, translation stage 9 and electrode 4 successively, is driven and is lifted by coordination electrode 4
Platform 8, translation stage 9 realize lifting, the translation of sample limiting plate 1 and EP pipe supports 3 respectively.By lifting, sample limiting plate 1 can be made
It is moved to the position of sample processor requirement, automatically processing and collecting for sample is realized by translation.
Through-hole internal diameter on the sample limiting plate 1 is identical.
The internal diameter of the through-hole of the left end of the sample support plate 2 is greater than or equal to the through-hole on the sample limiting plate 1
Internal diameter.
The distance of center circle between through-hole on the sample limiting plate 1 is 12mm~20mm.
Preferably, the distance of center circle between the through-hole on the sample limiting plate 1 is 14mm.
The depth of through-hole on the sample limiting plate 1 is 0.5mm~2.0mm.
Preferably, the depth of the through-hole on the sample limiting plate 1 is 1mm.
Beneficial effects of the present invention：It is transmitted and is collected with sample using a kind of microorganism mutation breeding instrument of the present invention and filled
It sets, passes through the mutual cooperation between sample limiting plate, sample support plate, EP pipe supports, it is only necessary to which motor moves in one direction
The function of automatically delivering and collect sample can be realized, accelerate the processing speed of sample, effectively prevent sample for a long time
Etc. the microbiological contamination occurred during pending and culture ingredients from lossing the problems such as.
Description of the drawings
Fig. 1 is a kind of microorganism mutation breeding instrument sample transmission and the structural representation of collection device in the embodiment of the present invention
Figure.
Each label lists as follows in figure：
1- sample limiting plates；2- sample support plates；3-EP pipe supports；4- motors；5- guide rails；6- sliding blocks；7- limit switches；8- is lifted
Platform；9- translation stages.
Specific implementation mode
Plasma mutation breeding instrument chosen below is as a kind of microorganism mutation breeding instrument, and in conjunction with attached drawing to the present invention
It illustrates.
Fig. 1 gives the structural schematic diagram of a kind of transmission of microorganism mutation breeding instrument sample and collection device.The device
Including sample limiting plate 1, sample support plate 2 and EP pipe supports 3, the EP pipe supports 3 are fixed on vertical with the sample limiting plate 1
On tablet, the sample support plate 2 is close to 1 lower section of sample limiting plate, and the EP pipe supports 3 are located at 2 lower section of sample support plate, described
Equidistant through-hole is provided on sample limiting plate 1, the through-hole is corresponded with the EP pipes on EP pipe supports 3, the sample branch
There are one through-hole, the sample support plate 2 is fixed on the holder of vertical direction for the left end setting of fagging 2, the sample limit
Plate 1 is connect with lifting platform 8, translation stage 9 and electrode 4 successively.Sample support plate 2 is fixed and is close to sample limiting plate 1, makes sample
Through-hole on limiting plate 1 forms groove on sample support plate 2, for holding sample or specimen slides.
The operating process of the device is as follows：The through-hole and sample pending sample being placed sequentially on sample limiting plate 1
In the groove that support plate 2 is constituted, it is 1-6 that groove is numbered from left to right, places sample size and is not limited only to 6 shown in FIG. 1；It will
The EP pipes of sample are sequentially fixed at EP pipe supports 3 after being handled for collection, liquid medium can be added in advance in EP pipes, for protecting
Protect sample；By adjusting lifting platform 8, translation stage 9 and limit switch 7, the sample in No. 1 groove is moved to plasma
Device following position directly；The corresponding processing parameter of each sample is set on the touch screen of plasma mutation breeding instrument, then
Startup program handles sample；After having handled No. 1 sample, motor 4 is moved horizontally by rotating band movable slider 6 on guide rail 5, directly
The underface of plasma generator is moved to No. 2 samples.During being somebody's turn to do, sample limiting plate 1, EP pipe supports 3 and then move together
It is dynamic, but sample support plate 2 is fixed and is close to the lower section of sample limiting plate 1, so No. 1 sample in groove will not be in movement
It falls, only when No. 1 sample is moved to right over the through-hole of 2 left hand edge of sample support plate, is just fallen from through hole in the process
Into the corresponding EP pipes in underface, to realize sample treatment after collected function at once.Subsequent sample processing and
Collection process refers to No. 1 sample, and after whole samples are collected, motor 4 reversely rotates the band negative side on guide rail 5 of movable slider 6
To moving horizontally, stop movement when triggering limit switch 7.
The device can handle microbiological specimens and plant sample, wherein microbiological specimens include bacterium, it is actinomyces, mould
Bacterium, the bacteria suspension of saccharomycete or spore suspension etc.；Plant sample includes vegetable seeds, plant callus and plant seedlings etc.,
The scope of application is very wide.
Claims (9)
1. a kind of microorganism mutation breeding instrument is transmitted with sample and collection device, which is characterized in that the sample transmission and collection
Device includes sample limiting plate (1), sample support plate (2) and EP pipe supports (3), and the EP pipe supports (3) are fixed on and the sample
On the vertical tablet of limiting plate (1), the sample support plate (2) is close to below sample limiting plate (1), EP pipe supports (3) position
Below sample support plate (2), it is provided with equidistant through-hole on the sample limiting plate (1), the through-hole is in sample support
Groove is formed on plate (2), the through-hole is corresponded with the EP pipes on EP pipe supports (3), and the left end of the sample support plate (2) is set
Through-hole there are one setting, is moved to the left the sample limiting plate (1) and EP pipe supports (3), so that the sample in the groove is reached specific
Position is handled, and the sample after handling passes sequentially through the through-hole of sample support plate (2) left end again, falls into EP pipe supports (3)
In EP pipes.
2. sample transmission as described in claim 1 and collection device, which is characterized in that the sample support plate (2) is fixed on
On holder.
3. sample as described in claim 1 transmission and collection device, which is characterized in that the sample limiting plate (1) successively with
Lifting platform (8), translation stage (9) and electrode (4) connection drive lifting platform (8), translation stage (9), respectively by coordination electrode (4)
Realize lifting, the translation of sample limiting plate (1) and EP pipe supports (3).
4. sample transmission as described in claim 1 and collection device, which is characterized in that logical on the sample limiting plate (1)
Hole internal diameter is identical.
5. sample transmission as claimed in claim 4 and collection device, which is characterized in that sample support plate (2) left end
The internal diameter of through-hole is greater than or equal to the internal diameter of the through-hole on the sample limiting plate (1).
6. sample transmission according to any one of claims 1 to 5 and collection device, which is characterized in that the sample limiting plate (1)
On through-hole between distance of center circle be 12mm~20mm.
7. sample transmission as claimed in claim 6 and collection device, which is characterized in that logical on the sample limiting plate (1)
Distance of center circle between hole is 14mm.
8. sample transmission according to any one of claims 1 to 5 and collection device, which is characterized in that the sample limiting plate (1)
On through-hole depth be 0.5mm~2.0mm.
9. sample transmission as claimed in claim 8 and collection device, which is characterized in that logical on the sample limiting plate (1)
The depth in hole is 1mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710177112.8A CN108624496B (en) | 2017-03-23 | 2017-03-23 | Sample conveying and collecting device for microorganism mutation breeding instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710177112.8A CN108624496B (en) | 2017-03-23 | 2017-03-23 | Sample conveying and collecting device for microorganism mutation breeding instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108624496A true CN108624496A (en) | 2018-10-09 |
| CN108624496B CN108624496B (en) | 2022-01-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710177112.8A Active CN108624496B (en) | 2017-03-23 | 2017-03-23 | Sample conveying and collecting device for microorganism mutation breeding instrument |
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| Country | Link |
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| CN (1) | CN108624496B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760220A (en) * | 2019-11-01 | 2021-05-07 | 洛阳华清天木生物科技有限公司 | Automatic sample separating, processing and collecting device |
Citations (9)
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|---|---|---|---|---|
| WO2003021230A2 (en) * | 2001-09-05 | 2003-03-13 | Essen Instruments, Inc. | High-throughput electrophysiological measurement system |
| CN201186931Y (en) * | 2008-03-14 | 2009-01-28 | 清华大学 | Atmosphere pressure low temperature plasma breeding apparatus |
| CN102382763A (en) * | 2011-12-20 | 2012-03-21 | 北京思清源生物科技有限公司 | Plasma microbial mutagenic breeding equipment |
| CN103461117A (en) * | 2013-09-24 | 2013-12-25 | 河北科技大学 | Full-automatic physical mutation breeding machine |
| CN203424106U (en) * | 2013-09-02 | 2014-02-12 | 付艳 | Ultraviolet mutation breeding box |
| CN103981091A (en) * | 2014-05-23 | 2014-08-13 | 无锡思清源生物科技有限公司 | Novel normal-pressure room-temperature plasma induced mutation breeding device |
| CN104017718A (en) * | 2013-12-31 | 2014-09-03 | 无锡思清源生物科技有限公司 | Carrier disc and bacterium carrying device for disposable microorganism mutation breeding instrument |
| CN104841640A (en) * | 2015-04-30 | 2015-08-19 | 清华大学 | Automatic sorting device for irradiated particles |
| CN206940850U (en) * | 2017-03-23 | 2018-01-30 | 洛阳华清天木生物科技有限公司 | A kind of microbiological specimens transmission and collection device |
-
2017
- 2017-03-23 CN CN201710177112.8A patent/CN108624496B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003021230A2 (en) * | 2001-09-05 | 2003-03-13 | Essen Instruments, Inc. | High-throughput electrophysiological measurement system |
| CN201186931Y (en) * | 2008-03-14 | 2009-01-28 | 清华大学 | Atmosphere pressure low temperature plasma breeding apparatus |
| CN102382763A (en) * | 2011-12-20 | 2012-03-21 | 北京思清源生物科技有限公司 | Plasma microbial mutagenic breeding equipment |
| CN203424106U (en) * | 2013-09-02 | 2014-02-12 | 付艳 | Ultraviolet mutation breeding box |
| CN103461117A (en) * | 2013-09-24 | 2013-12-25 | 河北科技大学 | Full-automatic physical mutation breeding machine |
| CN104017718A (en) * | 2013-12-31 | 2014-09-03 | 无锡思清源生物科技有限公司 | Carrier disc and bacterium carrying device for disposable microorganism mutation breeding instrument |
| CN103981091A (en) * | 2014-05-23 | 2014-08-13 | 无锡思清源生物科技有限公司 | Novel normal-pressure room-temperature plasma induced mutation breeding device |
| CN104841640A (en) * | 2015-04-30 | 2015-08-19 | 清华大学 | Automatic sorting device for irradiated particles |
| CN206940850U (en) * | 2017-03-23 | 2018-01-30 | 洛阳华清天木生物科技有限公司 | A kind of microbiological specimens transmission and collection device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760220A (en) * | 2019-11-01 | 2021-05-07 | 洛阳华清天木生物科技有限公司 | Automatic sample separating, processing and collecting device |
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| Publication number | Publication date |
|---|---|
| CN108624496B (en) | 2022-01-25 |
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