CN108619273B

CN108619273B - Preparation method of plant fruit core oral liquid for treating helicobacter pylori

Info

Publication number: CN108619273B
Application number: CN201810594838.6A
Authority: CN
Inventors: 王为; 王蒙; 兰涛
Original assignee: Tianjin Yunxin Technology Co ltd
Current assignee: Tianjin Yunxin Technology Co ltd
Priority date: 2018-06-11
Filing date: 2018-06-11
Publication date: 2021-05-28
Anticipated expiration: 2038-06-11
Also published as: CN108619273A

Abstract

The invention provides a preparation method of a plant fruit stone oral liquid for treating helicobacter pylori, which comprises the following steps of 1) selecting plant polysaccharide with helicobacter pylori resistance activity, putting the plant polysaccharide into a fruit stone vinegar refined liquid, wherein the input amount of the plant polysaccharide accounts for 5-30% of the mass of the fruit stone vinegar refined liquid, and uniformly stirring to obtain the fruit stone polysaccharide vinegar liquid; 2) adding plant protease into the fruit stone polysaccharide vinegar liquid obtained in the step 1) for enzymolysis reaction, wherein the reaction temperature is 50-60 ℃, the reaction time is 30-45min, and the addition amount of the enzyme is 400-600U/mL; 3) filtering the enzymolysis-finished fruit stone polysaccharide vinegar liquid treated in the step 2) to obtain the oral liquid for treating the helicobacter pylori. The oral liquid has strong antibacterial and anti-inflammatory effects and has strong inhibition effect on helicobacter pylori.

Description

Preparation method of plant fruit core oral liquid for treating helicobacter pylori

Technical Field

The invention belongs to the technical field of an oral liquid for treating helicobacter pylori, and particularly relates to a preparation method of a plant fruit core oral liquid for treating helicobacter pylori.

Background

The investigation result proves that nearly half of the population is infected with helicobacter pylori in the world, the infection ability of the disease is extremely strong, and the disease can be transmitted by hands, unclean tableware, unclean food, excrement and the like. Generally, a single antibacterial drug is not advocated at present because the cure rate is low, drug resistance is easy to generate and the like, some people consider that the treatment of the helicobacter pylori symptom is an easy problem, the treatment effect is not ideal in practice, and a suitable helicobacter pylori animal model is not available at present.

With the unification of understanding of diseases related to Hp infection, the treatment for eradicating Hp has been widely used clinically. Eradication is the detection of Hp in the stomach 1 month after treatment is complete. In an in vitro drug sensitivity test, many antibiotics have good antibacterial activity on Hp, but in a low pH environment in vivo, most of the antibiotics have reduced activity and cannot penetrate a mucous layer to achieve effective bactericidal concentration locally in bacteria, so that Hp infection is often difficult to eradicate clinically. To date, no single antibiotic has been able to effectively eradicate Hp. Thus, various treatment schemes combining antibiotics, bismuth agents and anti-secretory drugs have been developed.

Currently, triple or quadruple regimens are commonly used, standard triple regimens include PPI, clarithromycin and metronidazole or amoxicillin, and the 2016 consensus on toronto suggests a prolongation of the Hp treatment period to 14 days. In the Maastricht-4 consensus, where the local clarithromycin resistance rate is > 15% -20%, the use of standard triple therapy is not recommended and a tetrad regimen containing bismuth should be selected as first line therapy. Extension of the standard bismuth agent tetrad scheme: (1) bismuth agent + PPI + amoxicillin + clarithromycin; (2) bismuth agent + PPI + amoxicillin + furazolidone; (3) bismuth agent + PPI + amoxicillin + fluoroquinolone drugs. In recent years, clarithromycin, metronidazole and fluoroquinolone medicines have certain drug resistance.

The traditional Chinese medicine and western medicine for treating helicobacter pylori diseases are various at present, but the same three problems exist:

1. eradication criteria problem for Hp

There are two concepts of clearance (clearance) of Hp and eradication (eradication), the former referring to the negative of Hp at the end of treatment, the latter referring to the negative of Hp after 1 month of cessation of treatment. Clearance is only a temporary suppression of Hp, and Hp infection is relapsed soon after drug withdrawal; the eradication means that 85 percent of patients do not relapse within 1 day, and the treatment effect is achieved.

2. Effect of intragastric Hp on the therapeutic Effect of antibacterial drugs

The in vitro inhibitory concentration of the antibacterial agent and the in vivo antibacterial activity are not completely consistent. Whether an antibacterial drug can maintain its stable antibacterial activity in the acidic environment in the stomach will directly affect the efficacy of the treatment of Hp infection.

3. The problem of drug resistance of antibacterial drugs

Helicobacter pylori is a common pathogenic bacterium in the upper digestive tract, plays an important role in the pathogenesis of peptic ulcer, gastric cancer and the like, and the eradication of helicobacter pylori is an important link for treating the diseases. The existing method for eradicating helicobacter pylori is high in cost, and more serious, along with the wide action of antibiotics, more and more drug-resistant helicobacter pylori strains become a big problem which troubles the chemical industry.

How to fundamentally prevent the Hp infection and reduce the incidence of the Hp infection becomes a problem to be solved urgently.

Disclosure of Invention

In view of the above, the present invention aims to provide a method for preparing an oral liquid of plant fruit stone for treating helicobacter pylori, so as to overcome the defects of the prior art.

In order to achieve the purpose, the technical scheme of the invention is realized as follows:

a method for preparing plant fruit stone oral liquid for treating helicobacter pylori comprises the following steps,

1) selecting plant polysaccharide with anti-helicobacter pylori activity, adding the plant polysaccharide into the kernel vinegar refined liquid, wherein the adding amount of the plant polysaccharide accounts for 5-30% of the mass of the kernel vinegar refined liquid, and uniformly stirring to obtain kernel polysaccharide vinegar liquid;

2) adding plant protease into the fruit stone polysaccharide vinegar liquid obtained in the step 1) for enzymolysis reaction, wherein the reaction temperature is 50-60 ℃, the reaction time is 30-45min, and the addition amount of the enzyme is 400U-600U/mL;

3) filtering the enzymolysis-finished fruit stone polysaccharide vinegar liquid treated in the step 2) to obtain the oral liquid for treating the helicobacter pylori.

The obtained oral liquid mainly contains organic acids, polyphenols, ketones, alkaloids, saponins and VB group, contains numerous trace elements, and is a natural organic composition.

Mechanism of anti-Hp: because of the strong interaction of the penetration activity of natural combination of a plurality of active factors, the comprehensive effect of complete system engineering with sterilization, inflammation diminishing, cancer resistance, restoration, vitality generation, immunity and the like is formed, and the cure rate of HP diseases is doubled.

Preferably, the pit vinegar refined liquid is vinegar refined liquid of one or more than two kinds of pits of peach pit, date pit, hawthorn pit and wild jujube pit; the plant protease is papain.

Preferably, the plant polysaccharide is a mixture of lycium barbarum polysaccharide and hericium erinaceus polysaccharide, and the mass ratio of the lycium barbarum polysaccharide to the hericium erinaceus polysaccharide is 1: 1-1: 3.

Preferably, the kernel vinegar refined liquid used in the step 1) is prepared by the following steps,

a) drying and dehydrating the plant seeds until the water content is 4-18%, then placing the plant seeds in a pyrolysis furnace, supplying oxygen by using anhydrous air in the furnace, carrying out micro-aerobic combustion and pyrolysis on volatile matters escaping, separating the volatile matters by using a gas-solid separator, sending the separated gas volatile matters into a cooler for cooling, collecting condensable liquid, obtaining crude fruit pit vinegar liquid, and cooling; when the pH value of the liquid reaches 3-6, stopping collecting;

b) putting the kernel vinegar crude liquid obtained in the step a) into a distillation kettle, and distilling; after cooling, the mixture enters a recovery tank;

c) adding blanket-shaped active carbon fiber into the crude kernel vinegar liquid distilled in the step b), stirring for 1-30 minutes, standing for 24-36 hours, and filtering to obtain refined kernel vinegar liquid;

preferably, in the step a), the temperature of the micro-aerobic combustion in the pyrolysis furnace is 150-350 ℃; the temperature after cooling is 20-80 ℃; in the step b), the distillation temperature is 50-97 ℃.

Preferably, in step c), the specification of the carpet active carbon fiber is 60-80 meshes; the using amount of the blanket active carbon fiber is 1 to 15 percent of the mass of the kernel vinegar crude liquid distilled in the step b).

The invention also provides application of the oral liquid obtained by the preparation method of the plant fruit stone oral liquid for treating helicobacter pylori in preparation of medicines for treating or preventing stomach diseases caused by helicobacter pylori.

The invention also provides the application of the oral liquid obtained by the preparation method of the plant fruit stone oral liquid for treating the helicobacter pylori in preparing the anti-peptic ulcer medicaments for treating or preventing the helicobacter pylori.

The invention also provides the application of the oral liquid obtained by the preparation method of the plant fruit stone oral liquid for treating the helicobacter pylori in preparing a medicament for treating or preventing intestinal diseases caused by the helicobacter pylori.

Through comparative observation of different treatment schemes, the product is found to have strong antibacterial and anti-inflammatory effects and strong inhibition effect on helicobacter pylori. The polyphenol contained in the compound has obvious inhibition effect on various bacteria, fungi and yeasts, particularly has strong inhibition capability on common pathogenic bacteria such as cholera bacteria, staphylococcus aureus, escherichia coli and the like, and does not influence the growth and development of organisms. The polyphenol substance can be used as ingredient of medicine for treating gastritis and ulcer, and can inhibit the growth of helicobacter pylori. On one hand, the product has very strong killing and inhibiting effects on bacteria in intestinal tracts, and on the other hand, the product has a protective effect on beneficial bacteria in the intestinal tracts, such as promoting the growth and reproduction of bifidobacteria in the intestinal tracts, improving the microbial structure in the intestinal tracts of organisms, improving the intestinal immunity and improving the health. The polysaccharide is a natural plant polysaccharide, consists of 6 monosaccharides such as glucose, galactose, mannose, arabinose and the like, is proteoglycan containing various trace elements and amino acids, has rich biological activity and has no toxic or side effect; the active part of the glucan is glucan which is formed by connecting a main chain connected by beta (1 → 3) with a beta (1 → 6) bond; the action mechanism is that the phagocytosis of macrophages is increased, the formation of immunoglobulin is promoted, the regeneration and the repair of gastric mucosal epithelium are played an important role, and the barrier function of the gastric mucosa can be enhanced. Has good therapeutic effects on flatulence, belch, acid regurgitation, hematochezia, and anorexia. Because of the strong interaction of the penetration activity of the natural combination of a plurality of active factors, the comprehensive effect of complete system engineering with sterilization, inflammation diminishing, cancer resistance, restoration, vitality generation, immunity and the like is formed, and the cure rate of the helicobacter pylori disease is doubled.

The natural plant kernel extract, namely the kernel polysaccharide enzyme, is prepared by taking natural plant kernel extraction essence, various plant polysaccharides, protease and the like as raw materials, efficiently and completely extracting nutrient components in the raw materials through long-time microbial fermentation, converting the nutrient components into 100% of small molecules which are easy to be absorbed by a human body, scientifically proportioning the raw materials, and comprehensively and scientifically supplementing nutrients required by the human body, thereby being a natural plant nutrient drink.

Compared with the prior art, the preparation method of the plant fruit stone oral liquid for treating helicobacter pylori has the following advantages:

(1) the oral liquid prepared by the preparation method of the oral liquid has broad-spectrum anti-helicobacter pylori efficacy, successfully inhibits the survival diffusion of human HP cells, does not harm normal cells, and has definite selective targeting.

(2) The oral liquid obtained by the invention has broad-spectrum sterilization and inflammation diminishing effects, and can effectively kill inflammatory cells corroded by pathological changes;

(3) the oral liquid obtained by the invention has the function of a nutritional agent, activates normal tissue cells, enables immune cells to proliferate in a large amount, effectively eats harmful cell particles, reduces minaline inflammation and repairs damaged mucosa and tissues.

(4) The oral liquid has natural far infrared radiation function, and this radiation effect can promote the circulation of matter inside human body. Therefore, the cell membrane has the advantages of high activity of aqueous solution, strong penetrability of cell membrane and quick absorption by organisms; anti-cancer, sterilization, anti-inflammation, vital generation and repair are carried out synchronously.

(5) The oral liquid obtained by the invention can be continuously used for a long time, continuously improves symptoms, gradually recovers health, improves life quality and prolongs life cycle.

Detailed Description

Unless defined otherwise, technical terms used in the following examples have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.

The present invention will be described in detail with reference to examples.

Example 1

A method for preparing oral liquid for treating helicobacter pylori comprises the following steps,

1) selecting plant polysaccharide with anti-helicobacter pylori activity, adding the plant polysaccharide into the kernel vinegar refined liquid, wherein the adding amount of the plant polysaccharide accounts for 5% of the mass of the kernel vinegar refined liquid, and uniformly stirring to obtain kernel polysaccharide vinegar liquid; the plant polysaccharide is a mixture of lycium barbarum polysaccharide and hericium erinaceus polysaccharide, and the mass ratio of the lycium barbarum polysaccharide to the hericium erinaceus polysaccharide is 1: 2.

2) Adding papain into the fruit stone polysaccharide vinegar liquid obtained in the step 1) for enzymolysis reaction, wherein the reaction temperature is 50 ℃, the reaction time is 45min, and the addition amount of the enzyme is 600U/mL;

The kernel vinegar refining liquid used in the step 1) is prepared by the following steps,

a) drying and dehydrating the plant seeds until the water content is 15%, then placing the plant seeds in a pyrolysis furnace, supplying oxygen by using anhydrous air in the furnace, carrying out micro-aerobic combustion pyrolysis on volatile matters escaping, separating the volatile matters by using a gas-solid separator, sending the separated volatile matters into a cooler for cooling, collecting condensable liquid, obtaining the kernel vinegar crude liquid, and cooling; when the pH value of the liquid reaches 3-6, stopping collecting; the plant seeds are peach pits and wild jujube pits mixed according to the mass ratio of 7:3, and the temperature of micro-aerobic combustion in a pyrolysis furnace is 150-200 ℃; the temperature after cooling is 30 ℃;

b) putting the kernel vinegar crude liquid obtained in the step a) into a distillation kettle, and distilling; after cooling, the mixture enters a recovery tank; the distillation temperature was 75 ℃.

c) Adding blanket-shaped active carbon fiber into the crude kernel vinegar liquid distilled in the step b), stirring for 30 minutes, standing for 30 hours, and filtering to obtain refined kernel vinegar liquid; the specification of the blanket active carbon fiber is 60 meshes; the using amount of the blanket active carbon fiber is 15 percent of the mass of the kernel vinegar crude liquid distilled in the step b).

The oral liquid prepared in example 1 was used for treating peptic ulcer caused by helicobacter pylori, two sets of experiments were set, each set comprising 40 cases (age 30-50 years; duration 3-5 years), and positive detection was carried out by expiration method 13C-UBT before treatment;

in the comparison group, omeprazole 20mg, bismuth potassium citrate 2 bags and levofloxacin 0.2g are taken twice a day for 1 to 2 weeks as a treatment course. The patients in this group had better condition, but the recurrence rate of gastropathy reached 80%.

The experimental group took the oral liquid prepared in example 1, 30 ml/day 1 time a day, diluted with 3-10 times of warm boiled water and taken orally. After the medicine is continuously taken for three months, most of inflammation disappears, the detection is negative by an air blowing method 13C-UBT, and the cure rate reaches 98% in three months.

Example 2

1) selecting plant polysaccharide with anti-helicobacter pylori activity, adding the plant polysaccharide into the kernel vinegar refined liquid, wherein the adding amount of the plant polysaccharide accounts for 10% of the mass of the kernel vinegar refined liquid, and uniformly stirring to obtain the kernel polysaccharide vinegar liquid; the plant polysaccharide is a mixture of lycium barbarum polysaccharide and hericium erinaceus polysaccharide, and the mass ratio of the lycium barbarum polysaccharide to the hericium erinaceus polysaccharide is 1: 2.

a) drying and dehydrating the plant seeds until the water content is 15%, then placing the plant seeds in a pyrolysis furnace, supplying oxygen by using anhydrous air in the furnace, carrying out micro-aerobic combustion pyrolysis on volatile matters escaping, separating the volatile matters by using a gas-solid separator, sending the separated volatile matters into a cooler for cooling, collecting condensable liquid, obtaining the kernel vinegar crude liquid, and cooling; when the pH value of the liquid reaches 3-6, stopping collecting; the plant seeds are peach pits and date pits, and the mass ratio of the peach pits to the date pits is 7:3, mixing; the temperature of micro-aerobic combustion in the pyrolysis furnace is 250-300 ℃; the temperature after cooling is 30 ℃;

b) putting the kernel vinegar crude liquid obtained in the step a) into a distillation kettle, and distilling; after cooling, the mixture enters a recovery tank; the distillation temperature is 75 ℃;

The oral liquid prepared in example 2 is used for treating chronic gastritis caused by helicobacter pylori, two groups of experiments are set, and each group comprises 40 cases (the age is 40-60 years; the disease duration is 5-15 years); before treatment, chronic superficial gastritis was diagnosed.

The contrast group is taken, 1 package of bismuth potassium citrate is taken each time, and 3-4 times a day; clarithromycin, 0.25g of which is usually used once, and 1 time per 12 hours; famotidine 20 mg/time 2 times/day 1 twice, and the treatment course is 4-6 weeks. The patients in this group did not have any improvement.

The experimental group took the oral liquid prepared in example 1, 40 ml/day 2 times a day, diluted with 3-10 times of warm boiled water and taken orally. After continuous taking for one month, the symptoms are obviously relieved, and the cure rate reaches 100 percent after three months.

Example 3

1) selecting plant polysaccharide with anti-helicobacter pylori activity, adding the plant polysaccharide into the kernel vinegar refined liquid, wherein the adding amount of the plant polysaccharide accounts for 20% of the mass of the kernel vinegar refined liquid, and uniformly stirring to obtain the kernel polysaccharide vinegar liquid; the plant polysaccharide is a mixture of lycium barbarum polysaccharide and hericium erinaceus polysaccharide, and the mass ratio of the lycium barbarum polysaccharide to the hericium erinaceus polysaccharide is 1: 2.

a) drying and dehydrating the plant seeds until the water content is 15%, then placing the plant seeds in a pyrolysis furnace, supplying oxygen by using anhydrous air in the furnace, carrying out micro-aerobic combustion and pyrolysis on volatile matters escaping, separating the volatile matters by using a gas-solid separator, sending the separated gas volatile matters into a cooler for cooling, collecting condensable liquid, obtaining the kernel vinegar crude liquid, and cooling; when the pH value of the liquid reaches 3-4, stopping collecting; the plant seeds are peach kernels and hawthorn kernels, and the weight ratio of the plant seeds to the peach kernels is 7:3, the micro-oxygen combustion temperature in the pyrolysis furnace is 300-350 ℃; the temperature after cooling is 30 ℃;

The oral liquid prepared in example 3 was used for treating duodenal ulcer caused by helicobacter pylori, two experiments were conducted, each of 40 cases (age 50-65 years; duration 10-20 years), and before treatment, gastroscopy and helicobacter pylori infection were examined. A clear diagnosis of gastroduodenal bulbar ulcer with helicobacter pylori infection;

control, treatment was as follows: 20mg of omeprazole (or Xinxin, lansoprazole and the like) 220 mg of lizhudele, 1.0 g of amoxicillin and 0.5 g of clarithromycin, wherein the amoxicillin and the clarithromycin are respectively used once in the morning and at night every day for 14 days, the symptoms are not completely relieved, and the recurrence rate is 80%.

The experimental group takes the fructosan inhibitor 80 ml/day 2 times a day for three months continuously, the symptom is obviously relieved after taking for one week, and the patient is recovered and does not relapse after taking for 3 months.

In addition, the components of the oral liquids prepared in examples 1 to 3 were examined, and the component contents thereof were as follows:

the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. A preparation method of plant fruit stone oral liquid for treating helicobacter pylori is characterized in that: comprises the following steps of (a) carrying out,

2) adding plant protease into the fruit stone polysaccharide vinegar liquid obtained in the step 1) for enzymolysis reaction, wherein the reaction temperature is 50-60 ℃, the reaction time is 30-45min, and the addition amount of the enzyme is 400-600U/mL;

3) filtering the enzymolysis-finished fruit stone polysaccharide vinegar liquid treated in the step 2) to obtain an oral liquid for treating helicobacter pylori;

the pit vinegar refined liquid is a vinegar refined liquid of peach pit and jujube pit, or a vinegar refined liquid of peach pit and hawthorn pit, or a vinegar refined liquid of peach pit and wild jujube pit;

the plant protease is papain; the plant polysaccharide is a mixture of lycium barbarum polysaccharide and hericium erinaceus polysaccharide, and the mass ratio of the lycium barbarum polysaccharide to the hericium erinaceus polysaccharide is 1: 1-1: 3;

c) adding blanket-shaped active carbon fiber into the crude kernel vinegar liquid distilled in the step b), stirring for 1-30 minutes, standing for 24-36 hours, and filtering to obtain refined kernel vinegar liquid.

2. The method for preparing a plant stone oral liquid for the treatment of helicobacter pylori according to claim 1, wherein: in the step a), the temperature of the micro-aerobic combustion in the pyrolysis furnace is 150-350 ℃; the temperature after cooling is 20-80 ℃; in the step b), the distillation temperature is 50-97 ℃.

3. The method for preparing a plant stone oral liquid for the treatment of helicobacter pylori according to claim 1, wherein: in the step c), the specification of the blanket-shaped active carbon fiber is 60-80 meshes; the using amount of the blanket-shaped active carbon fiber is 1-15% of the mass of the kernel vinegar crude liquid subjected to the distillation treatment in the step b).

4. Use of the plant fruit stone oral liquid for treating helicobacter pylori according to any one of claims 1 to 3, in the preparation of a medicament for treating or preventing gastric diseases caused by helicobacter pylori.

5. Use of the oral liquid obtained by the method for preparing the plant fruit stone oral liquid for treating helicobacter pylori according to any one of claims 1 to 3 in the preparation of a medicament for treating or preventing peptic ulcer caused by helicobacter pylori.

6. Use of the oral liquid obtained by the method for preparing the plant fruit stone oral liquid for treating helicobacter pylori according to any one of claims 1 to 3 in the preparation of a medicament for treating or preventing intestinal diseases caused by helicobacter pylori.