CN108618870A - A kind of tissue engineering artificial heart valve prosthesis - Google Patents
A kind of tissue engineering artificial heart valve prosthesis Download PDFInfo
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- CN108618870A CN108618870A CN201710173180.7A CN201710173180A CN108618870A CN 108618870 A CN108618870 A CN 108618870A CN 201710173180 A CN201710173180 A CN 201710173180A CN 108618870 A CN108618870 A CN 108618870A
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- leaflet
- heart valve
- valve prosthesis
- artificial heart
- tissue engineering
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/24—Heart valves ; Vascular valves, e.g. venous valves; Heart implants, e.g. passive devices for improving the function of the native valve or the heart muscle; Transmyocardial revascularisation [TMR] devices; Valves implantable in the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/24—Heart valves ; Vascular valves, e.g. venous valves; Heart implants, e.g. passive devices for improving the function of the native valve or the heart muscle; Transmyocardial revascularisation [TMR] devices; Valves implantable in the body
- A61F2/2412—Heart valves ; Vascular valves, e.g. venous valves; Heart implants, e.g. passive devices for improving the function of the native valve or the heart muscle; Transmyocardial revascularisation [TMR] devices; Valves implantable in the body with soft flexible valve members, e.g. tissue valves shaped like natural valves
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2240/00—Manufacturing or designing of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
- A61F2240/001—Designing or manufacturing processes
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- Health & Medical Sciences (AREA)
- Cardiology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Transplantation (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Vascular Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Prostheses (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a kind of tissue engineering artificial heart valve prosthesis, including valve frame and at least two panels leaflet and annulus that are attached on the valve frame, the valve frame are fixed on the upper of annulus, and lower edge and the lateral edges of every leaflet are fixed on the inside of valve frame；Wherein, the leaflet has several holes through leaflet upper and lower surface；There is biological tissue's layer, biological tissue layer to wrap up the leaflet and form cell material compound with the leaflet at the surface of the leaflet and hole；Biological tissue's layer is to obtain biopsy cell from body and obtained by cultivation method.The heart valve prosthesis prosthese of the technical program has good biocompatibility, and after being implanted into human body, blood coagulation and inflammation risk substantially reduce.
Description
Technical field
The present invention relates to field of medical technology, especially a kind of tissue engineering artificial heart valve prosthesis.
Background technology
Valvulopathy is common one of the heart disease of China, wherein predominantly valve lesions caused by rheumatic fever.In recent years
Come the development with aging of population, valve retrogression pathological changes (including calcification) and dysbolism valve lesions in China
It is increasing.
The heart of human body includes 4 valves, bicuspid valve, tricuspid valve, aorta petal and pulmonary valve.It is with bicuspid valve
Example, mitral position is between atrium sinistrum and left ventricle.It is a unidirectional valve.When diastole, bicuspid valve is opened, blood
Left ventricle is flowed to by atrium sinistrum, when heart contraction, mitral valve closure, blood from left ventricle through left ventricular outflow tract view, aorta pump to
Whole body.When lesion occurs with mitral valve, such as calcification can lead to valvular sclerosis, to can not normal switching-off, this phenomenon I
Be referred to as valvular inadequacy.Valvular inadequacy can cause in heart contraction, and part blood blows back into atrium sinistrum, referred to as
For valvular regurgitation.Valvular regurgitation increases the load of heart, can cause heart failure, causes patient dead when serious.
It is row cardiac valve replacement to treat a kind of common method of valvulopathy.I.e. manually heart valve replaces and looks for original
There is the valve of lesion.Common heart valve prosthesis can generally be divided into two major classes：Mechanical valve prosthesis and biovalve.Mechanical prosthetic valve
The leaflet of film is made of metal material, such as：Titanium alloy.Mechanical valve prosthesis has high intensity and fatigue performance, Huan Zhezhi
It can be used for a long time after entering, but need to take anticoagulation throughout one's life, to reduce the risk of thrombosis.Taking anticoagulation for a long time can bring
The adverse reactions such as people digest's gastrointestinal hemorrhage.
The leaflet material of biovalve is often derived from for example：The pericardium of ox or the valve of pig.By certain chemical treatment
After sew on metallic support.This heart valve prosthesis made of biomaterial has good biocompatibility, implantation
Afterwards, patient normally only needs to take 6 months anticoagulations, greatly reduces the risk of thrombosis and avoids and is caused by anticoagulation
Side effect.But there is also certain problems for this valve, after the certain time that implants, such as：10 years, often occur
Calcification decays, and valve is caused to can not work normally.Patient needs row second operation.Second operation increases patient's death
Risk.
The shortcomings that in order to overcome mechanical valve prosthesis and biovalve, the valve by artificial polymer's material preparation are developed.
A kind of heart prepared by ultra-high molecular weight polyethylene braided fabric is described for example, patent announcement number is CN103998208A
Vascular implantation prosthese.
United States Patent (USP) US50224231A1 describes a kind of polymer porous material, and filling elastic material is made again in hole
Condensation material is prepared into valve prosthesis using the composite material.
Chinese patent notification number CN1228295A describes a kind of valve made of elasticity teflon fibre braided object
Film prosthese.
According to the literature with some clinical datas, these artificial polymer's valves have good compared to biovalve
Anti-fatigue performance can reach the longer effective time；Compared to mechanical valve prosthesis, anticoagulation function also wants excellent many.
But artificial polymer's material is still foreign matter for human body, there are the wind of thrombosis and inflammatory reaction after implanting
Danger.
Therefore, above-mentioned technical problem needs to solve.
Invention content
The present invention is for overcome the deficiencies in the prior art, it is proposed that a kind of tissue engineering artificial heart valve prosthesis, purpose
It is to improve the biocompatibility of heart valve prosthesis.
In order to solve the above technical problems, basic technical scheme proposed by the present invention is：A kind of tissue engineering artificial heart
Dirty valve prosthesis, including valve frame and at least two panels leaflet that is attached on the valve frame, which is characterized in that further include：
One annulus, the valve frame are fixed on the upper of annulus, and lower edge and the lateral edges of every leaflet are fixed on valve frame
Inside；
Wherein, the leaflet has several holes through leaflet upper and lower surface；
Wherein, there is biological tissue's layer at the surface of the leaflet and hole, biological tissue layer is by the leaflet packet
It wraps up in and forms cell-material composite with the leaflet；
Wherein, biological tissue's layer is to obtain biopsy cell from body and obtained by cultivation method.
Further, including the identical or different leaflet of three chip architectures, three pieces leaflet are symmetrically distributed on the valve frame.
Further, the aperture of the hole in the leaflet is less than 5 μm.
Further, the porosity of the leaflet is between 10% to 50%.
Further, the thickness of every leaflet is less than 350 μm.
Further, the thickness of biological tissue layer between 0.1mm between 1.5mm.
Further, the biopsy cell is endothelial cell or the fibroblast of body.
Further, the leaflet is made for artificial synthesizing polymeric material, which is superelevation
One kind in molecular weight polyethylene, high density polyethylene (HDPE), polytetrafluoroethylene (PTFE), terylene, polyurethane.Further, the leaflet is
It is 100um that thickness, which is made, by plain weave by the polyester fiber of 50 denier, and average fiber spacing is 4um, and porosity is 50%
Terylene woven object.
Further, the cultural method of biological tissue's layer includes the following steps：
(1) after the male rabbit that will grow up is anaesthetized with yellow Jackets (35mg/kg), aorta is aseptically taken out, is put into
In PBS solution；Blood vessel is routed up after washing down, is put into the DMEM trainings of the I of clostridiopetidase A containing 1mg/ml types, 4mg/ml bovine serum albumins
In nutrient solution, it is placed in 37 DEG C, places 30 minutes under the conditions of 5%CO2；
(2) digestive juice is collected and carries out centrifugal treating, the rabbit aorta endothelial cell for then obtaining centrifugation is blown and beaten equal
It is even, it is added in the DMEM culture solutions containing 20% newborn bovine serum；When cell fusion is single layer, with 0.125% trypsase
Had digestive transfer culture；
(3) endothelial cell is digested through 2.5g/L pancreatin, and obtained counting concentration of cell suspension is 3 × 108/L, by this cell
Suspension is added in the DMEM culture solutions for being soaked with terylene woven beyond the region of objective existence section valve, is placed in 37 DEG C of incubators, is cultivated 8 days；Obtained group
Weaver's journey terylene woven object valve is impregnated with a concentration of 3% glutaraldehyde；Endothelial tissue is uniformly distributed in terylene woven securely
In object leaflet, terylene woven object is wrapped by intact.
The beneficial effects of the invention are as follows：
Technical scheme of the present invention, a kind of tissue engineering artificial heart valve prosthesis, including valve frame, at least two panels leaflet and
Annulus, valve frame are fixed in annulus, and at least two panels leaflet is attached on the valve frame；It is trained in at least two panels leaflet
Biological tissue's layer is given birth to, biological tissue layer forms cell-material composite with leaflet.This technology is compared to traditional bioprosthetic valves
Film, valve provided by the invention have longer service life；Compared to traditional mechanical valve prosthesis, valve provided by the invention is not
It needs to take anticoagulant throughout one's life；Compared to traditional artificial-synthetic copolymer's material valve, valve biology provided by the invention
Compatibility is more excellent, and the risk of blood coagulation and inflammation is greatly reduced after implantation.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of tissue engineering artificial heart valve prosthesis of the present invention.
Specific implementation mode
Below with reference to attached drawing 1 and embodiment, the present invention is described further, but should not limit the present invention with this
Protection domain.
Embodiment 1
A kind of tissue engineering artificial heart valve prosthesis of the present invention, performs the operation for cardiac replacement.The prosthese includes valve frame 10
With at least two panels leaflet 20 being attached on the valve frame 10, the valve frame 10 is fixed in an annulus 30.Wherein, described
The lower edge of every leaflet 20 is fixed on the inside of valve frame 10 with lateral edges.In the present embodiment, using three pieces leaflet 20, this three
Its identical either different shape of structure of piece leaflet 20 are completely the same or different.Specifically, three pieces leaflet 20 is uniformly arranged on
It is symmetrical in three equal parts on the valve frame 10.
Specifically, 30 inside of the annulus includes metal support, which is preferably cochrome, can also
It is stainless steel or titanium alloy or Nitinol.30 outer surface of annulus is high molecular material, such as is wrapped up by polyester fiber cloth.
The annulus 30 is fixed on human body annulus tissue for suturing, and plays the role of support.The valve frame 10 be titanium chrome alloy silk by
Mold bending is molded, and plays the role of supporting leaflet.
Specifically, in the present embodiment, the leaflet 20 has several holes through 20 upper and lower surface of leaflet；The leaflet
The aperture of hole on 20 is less than 5 μm；The porosity of the leaflet 20 is between 10% to 50%；The thickness of every leaflet 20
Less than 350 μm.
Wherein, the leaflet 20 is made for artificial synthesizing polymeric material, which is superelevation point
One kind in sub- weight northylen, high density polyethylene (HDPE), polytetrafluoroethylene (PTFE), terylene, polyurethane.Detailed, which is by 50
It is 100um that thickness, which is made, by plain weave in the polyester fiber of denier, and average fiber spacing is 4um, the terylene that porosity is 50%
Braided fabric；The leaflet 20 is process after cutting by above-mentioned terylene woven object, and the method for cutting is preferably to be cut by laser.Its
In, the leaflet 20 is fixed in the way of suture on above-mentioned valve frame 10.
As the emphasis of technical scheme of the present invention, there is biological tissue's layer at the surface of the leaflet 20 and hole,
The leaflet 20 is wrapped up and forms cell-material composite with the leaflet 20 by biological tissue layer；Wherein, the biological group
Tissue layer is to obtain biopsy cell from body and obtained by cultivation method.The thickness of biological tissue layer between
0.1mm is between 1.5mm；Wherein, the biopsy cell is endothelial cell or the fibroblast of body.
Leaflet 20 in the present embodiment is that thickness is made for 100um, averagely by plain weave in the polyester fiber of 50 denier
Fiber spacing (i.e. pore-size) is 4um, and the terylene woven object that porosity is 50%, Fig. 1 is made in suture to the braided fabric after cutting
Shown in surgery valve.The structure of valve frame 10, leaflet 20 and annulus 30 is as shown in Fig. 1.
The cultural method of biological tissue's layer includes the following steps：
(1) after the male rabbit that will grow up is anaesthetized with yellow Jackets (35mg/kg), aorta is aseptically taken out, is put into
In PBS solution；Blood vessel is routed up after washing down, is put into the DMEM trainings of the I of clostridiopetidase A containing 1mg/ml types, 4mg/ml bovine serum albumins
In nutrient solution, it is placed in 37 DEG C, places 30 minutes under the conditions of 5%CO2；
(2) digestive juice is collected and carries out centrifugal treating, the rabbit aorta endothelial cell for then obtaining centrifugation is blown and beaten equal
It is even, it is added in the DMEM culture solutions containing 20% newborn bovine serum；When cell fusion is single layer, with 0.125% trypsase
Had digestive transfer culture；
(3) endothelial cell is digested through 2.5g/L pancreatin, and obtained counting concentration of cell suspension is 3 × 108/L, by this cell
Suspension is added in the DMEM culture solutions for being soaked with terylene woven beyond the region of objective existence section valve, is placed in 37 DEG C of incubators, is cultivated 8 days；Obtained group
Weaver's journey terylene woven object valve is impregnated with a concentration of 3% glutaraldehyde；Endothelial tissue is uniformly distributed in terylene woven securely
In object leaflet, terylene woven object is wrapped by intact.The tissue thickness of sliced observation, leaflet surface is 1mm.By organizational project
The aorta petal of the sterilized implantation bull sheep of terylene woven object valve, does not carry out anticoagulant therapy after implantation.After two months
Follow-up samples, and sheep physical signs is good, and leaflet form is intact, and surface is not found without apparent thrombus, valve tissue around slice
Apparent inflammatory reaction.
Embodiment 2
The embodiment 2 is consistent with the prosthese basic structure of embodiment 1.Wherein 20 thickness of leaflet is 350um, and porosity is
30%, average pore size is the eptfe film of 5um, and suture after cutting is prepared into surgery valve shown in Fig. 1, valve
Membrane preparation method and structure are same as Example 1.
Cultivating fibroblastic method in terylene woven object leaflet using organizational project is：
(1) human abdomen's skin is cleaned with the PBS solution containing penicillin and streptomysin, removes subcutaneous fat, then
Skin is immersed in the DMEM culture solutions containing II Collagenase Types, fetal calf serum, penicillin and streptomysin, in 37 DEG C of saturated humidities
Lower overnight incubation；Next day picking sweat gland is cultivated under 37 DEG C of saturated humidities in sweat gland culture solution；
(2) when fibroblastic growth is apparent, old culture solution is abandoned in suction, and salt is balanced with the Hank of no sodium ion and magnesium ion
Solution is washed 2 times, and the D-Hank digestive juices containing pancreatin and ethylenediamine tetra-acetic acid are added and are digested to cell, then use ox blood containing tire
Clear DMEM culture solutions terminate digestion, and fibroblast is collected by centrifugation；
(3) with the DMEM culture solution cell dispersions containing fetal calf serum, penicillin and streptomysin, then above-mentioned varicosity is gathered
Tetrafluoroethene valve is put into the culture solution, is cultivated 10 days under the conditions of 37 DEG C of saturated humidities；Fibroblast is uniformly divided securely
In expanded PTFE leaflet, leaflet 20 is wrapped cloth by intact；The tissue thickness of sliced observation, 20 surface of leaflet is
1.5mm。
Embodiment 3
It by plain weave is 60um at thickness that leaflet 20, which is by the superhigh molecular weight polyethylene fibers of 10D, and porosity is
10%, average fiber spacing (i.e. pore-size) is the braided fabric of 2um.The braided fabric passes through with 1 identical method of embodiment, system
It is standby at leaflet to obtain ultra-high molecular weight polyethylene valve.The present embodiment is used with 1 identical Method of Tissue Engineering of embodiment,
By external culture in 1 day, endothelial tissue, tissue thickness 0.1mm are turned out in ultra-high molecular weight polyethylene leaflet.Endothelium
Ultra-high molecular weight polyethylene leaflet is wrapped up well organizedly, it is securely not easily to fall off.
Comparative example 1
This comparative example uses and 1 identical terylene woven object manufacturing artificial valve of embodiment, without organizational project culture,
Direct sterilized implantation bull sheep aorta petal, does not carry out anticoagulant therapy after implantation.Follow-up samples after two months, leaflet
Form is intact, but thrombus trace occurs in surface, and the histotomy of leaflet root shows a large amount of macrophages, show exist compared with
Serious inflammatory reaction.
Comparative example 2
This comparative example makes valve using void-free ptfe sheet, and leaflet thickness is 350um.By with implementation
2 identical fibroblast organizational project culture of example, there is apparent one layer of fibroblast on obtained leaflet surface, but holds very much
Easy to fall off or sliding, exposes polytetrafluoroethylene material.
Embodiment 1 and the comparison of comparative example 1 can be seen that tissue engineering technique on the surface of artificial-synthetic copolymer's leaflet
The endothelial tissue turned out, induces thrombus after so that leaflet 20 is implanted and the risk of inflammatory reaction substantially reduces, and naked terylene
After braided fabric enters in vivo, thrombosis is had, and cause certain inflammatory reaction.The comparison of embodiment 2 and comparative example 2 can be with
To find out, leaflet made of artificial-synthetic copolymer needs porous structure, and the growth convenient for cell when organizational project culture is sticked,
Smooth leaflet surface is unfavorable for cell adhesion, hardly results in stable organized layer.
Technical scheme of the present invention finds out have with respect to the heart valve prosthesis prosthese with traditional prosthese, the technical program
There is good biocompatibility, after being implanted into human body, blood coagulation and inflammation risk substantially reduce.
According to the disclosure and teachings of the above specification, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula is changed and is changed.Therefore, the invention is not limited in specific implementation modes disclosed and described above, to the present invention's
Some modifications and changes should also be as falling into the scope of the claims of the present invention.In addition, although being used in this specification
Some specific terms, these terms are merely for convenience of description, does not limit the present invention in any way.
Claims (10)
1. a kind of tissue engineering artificial heart valve prosthesis, including valve frame (10) and be attached on the valve frame (10) at least two
Piece leaflet (20), which is characterized in that further include：
One annulus (30), the valve frame (10) are fixed on the upper of annulus (30), the lower edge and lateral edges of every leaflet (20)
It is fixed on the inside of valve frame (10)；
Wherein, the leaflet (20) has several holes through leaflet (20) upper and lower surface；
Wherein, there is biological tissue's layer at the surface of the leaflet (20) and hole, biological tissue layer is by the leaflet
(20) it wraps up and forms cell-material composite with the leaflet (20)；
Wherein, biological tissue's layer is to obtain biopsy cell from body and obtained by cultivation method.
2. a kind of tissue engineering artificial heart valve prosthesis as described in claim 1, it is characterised in that：Including three chip architecture phases
Same or different leaflets (20), three pieces leaflet (20) are symmetrically distributed on the valve frame (10).
3. a kind of tissue engineering artificial heart valve prosthesis as described in claim 1, it is characterised in that：
The aperture of hole on the leaflet (20) is less than 5 μm.
4. a kind of tissue engineering artificial heart valve prosthesis as described in claim 1, it is characterised in that：
The porosity of the leaflet (20) is between 10% to 50%.
5. a kind of tissue engineering artificial heart valve prosthesis as described in claim 1, it is characterised in that：
The thickness of every leaflet (20) is less than 350 μm.
6. a kind of tissue engineering artificial heart valve prosthesis as described in claim 1, it is characterised in that：
The thickness of biological tissue's layer is between 0.1mm between 1.5mm.
7. a kind of tissue engineering artificial heart valve prosthesis as described in claim 1, it is characterised in that：
The biopsy cell is endothelial cell or the fibroblast of body.
8. a kind of tissue engineering artificial heart valve prosthesis as described in claim 1, it is characterised in that：
The leaflet (20) is made for artificial synthesizing polymeric material, which is superhigh molecular weight polyethylene
One kind in alkene, high density polyethylene (HDPE), polytetrafluoroethylene (PTFE), terylene, polyurethane.
9. a kind of tissue engineering artificial heart valve prosthesis as described in claim 1, it is characterised in that：The leaflet (20) is
It is 100um that thickness, which is made, by plain weave by the polyester fiber of 50 denier, and average fiber spacing is 4um, and porosity is 50%
Terylene woven object.
10. a kind of tissue engineering artificial heart valve prosthesis as described in one of claim 1 to 9, it is characterised in that：
The cultural method of biological tissue's layer includes the following steps：
(1) after the male rabbit that will grow up is anaesthetized with yellow Jackets (35mg/kg), aorta is aseptically taken out, it is molten to be put into PBS
In liquid；Blood vessel is routed up after washing down, is put into the DMEM culture solutions of the I of clostridiopetidase A containing 1mg/ml types, 4mg/ml bovine serum albumins
In, it is placed in 37 DEG C, places 30 minutes under the conditions of 5%CO2；
(2) digestive juice is collected and carries out centrifugal treating, the rabbit aorta endothelial cell for then obtaining centrifugation is blown and beaten uniformly, is added
Enter into the DMEM culture solutions containing 20% newborn bovine serum；When cell fusion is single layer, passed with 0.125% trypsin digestion
Generation；
(3) endothelial cell is digested through 2.5g/L pancreatin, and obtained counting concentration of cell suspension is 3 × 108/L, by this cell suspension
It is added in the DMEM culture solutions for being soaked with terylene woven beyond the region of objective existence section valve, is placed in 37 DEG C of incubators, cultivate 8 days；Obtained group weaver
Journey terylene woven object valve is impregnated with a concentration of 3% glutaraldehyde；Endothelial tissue is uniformly distributed in terylene woven object valve securely
Ye Shang, terylene woven object are wrapped by intact.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710173180.7A CN108618870A (en) | 2017-03-22 | 2017-03-22 | A kind of tissue engineering artificial heart valve prosthesis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710173180.7A CN108618870A (en) | 2017-03-22 | 2017-03-22 | A kind of tissue engineering artificial heart valve prosthesis |
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| Publication Number | Publication Date |
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| CN108618870A true CN108618870A (en) | 2018-10-09 |
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ID=63707158
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| CN201710173180.7A Pending CN108618870A (en) | 2017-03-22 | 2017-03-22 | A kind of tissue engineering artificial heart valve prosthesis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113274549A (en) * | 2021-06-11 | 2021-08-20 | 上海奈姿医疗美容门诊部有限公司 | Device and method for preparing cell membrane |
| CN116849873A (en) * | 2023-07-17 | 2023-10-10 | 苏州心岭迈德医疗科技有限公司 | Artificial high molecular heart valve |
-
2017
- 2017-03-22 CN CN201710173180.7A patent/CN108618870A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113274549A (en) * | 2021-06-11 | 2021-08-20 | 上海奈姿医疗美容门诊部有限公司 | Device and method for preparing cell membrane |
| CN116849873A (en) * | 2023-07-17 | 2023-10-10 | 苏州心岭迈德医疗科技有限公司 | Artificial high molecular heart valve |
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Application publication date: 20181009 |
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| WD01 | Invention patent application deemed withdrawn after publication |