CN108587913A - A kind of scenedesmus, its cultural method and its application with high ALA contents - Google Patents

A kind of scenedesmus, its cultural method and its application with high ALA contents Download PDF

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CN108587913A
CN108587913A CN201810337916.4A CN201810337916A CN108587913A CN 108587913 A CN108587913 A CN 108587913A CN 201810337916 A CN201810337916 A CN 201810337916A CN 108587913 A CN108587913 A CN 108587913A
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徐旭东
高宏
孟霞
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Institute of Hydrobiology of CAS
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Abstract

The present invention relates to the scenedesmus with high ALA contents in a kind of total fat, further relate to cultural method and the application of algae strain.The aliphatic acid of the total fat of scenedesmus of the present invention is mainly made of 16 carbon, oleic acid, linoleic acid and ALA, and the content of ALA is stablized under the condition of culture of optimization 35 45% in total fat.For example, carrying out the fermenting experiment of 3L, after 120h, the dry cell weight of culture can reach 19.48gL‑1, total lipid content 12.1%, the yield that the content of ALA is 42.42%, ALA in total fat is 0.2gL‑1d‑1.The additive that algae strain can be used for producing animal feed is also used as the raw material of preparation of industrialization ALA to meet the needs of growth of animal is to nutrition such as ALA.

Description

A kind of scenedesmus, its cultural method and its application with high ALA contents
Technical field
The present invention relates to microalgae and its culture technique, more specifically it relates to a kind of scenedesmus with high ALA contents, its training Support method and its application.
Background technology
Polyunsaturated fatty acid (Polyunsaturated Fatty Acid, PUFA) refers to containing two or more Double bond and the straight chain fatty acid that carbon length is 18-22 carbon atom include mainly linoleic acid (LA), gamma-Linolenic acid (GLA), α- Leukotrienes (ALA), arachidonic acid (AA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) etc..In PUFA, It is known as ω -3 (n-3) series on the 3rd carbon atom away from the farthest double bond of c-terminus, ω-is known as if on the 6th carbon atom 6 (n-6) series.
Alpha-linolenic acid (α-linolenic acid, ALA) is all-cis 9,12,15-octadecatrienoic acid, belongs to ω -3 systems Row polyunsaturated fatty acid, the extension by carbochain and further desaturation, may finally form eicosapentaenoic acid (eicosapentaenoic acid, EPA) and docosahexaenoic acid (docosahexaenoic acid, DHA).EPA is The precursor of triolefin prostaglandin (such as PGI3, TXA3) in object, DHA are then the main of the nervous systems phosphatide such as brain, retina Ingredient.ALA and its derivative EPA and DHA is for the brain development of animal, the signal transduction of nervous system, cardiovascular system Development and the important function of cellular anti-oxidant etc. recognized and received by the public.Since the mankind and other lactations are dynamic Object itself cannot synthesize ALA, and required ALA must be absorbed from food in vivo；Moreover, ω -3/ ω -6 aliphatic acid the ratios of intake The too low ALA that also results in of example cannot effectively be converted to DHA and EPA, lead to mental disease and angiocardiopathy.Therefore, it mends in time It is very necessary to the health of human and animal to fill alpha-linolenic acid.2011 food security office of European Union (EFSA) nutrition science announce In it is also proposed that, ALA and brain and neurological tissue development are closely related, it is proposed that ALA is added in ordinary meal.
Lubricant component of the omega-3 polyunsaturated fatty acids of the mankind in the foods such as plant and fish.The growth of fish Development is also required to the serial polyunsaturated fatty acids of ω -3, and the algae to ingest and other plant are mostly come from nature.Research Show that ALA is added in feed can meet the needs of cultured fishes are for this kind of aliphatic acid, promote growth and health.
Microalgae is a kind of unicellular or many cells rudimentary plant.Numerous species are rich in protein, grease, polysaccharide in microalgae Etc. nutritional ingredients, type also is rich in physiologically active ingredients such as unsaturated fatty acid, carotenoid, vitamins.Spirulina, The algae such as chlorella dry powder can remarkably promote the growth, metabolism and premunition of aquatic livestock as feed addictive.But mesh Preceding microalgae industry relies primarily on autophyting growth and carries out open pond culture, and overall productivity is low, price is high, can only meet higher-end Consumption, it is difficult to be used for the demand of aquatic animal feed industry additive, and the algaes ALA contents such as spirulina are very low.For this purpose, needing Screen rich in ALA, can heterotrophic growth algae, develop the fermentation technique of microalgae, greatly improve unit area microalgae yield. The present invention be to provide it is a kind of be not only rich in ALA again can complete heterotrophic growth scenedesmus (Scenedesmus sp.), and ferment Training systern.
Priority claim
This application claims the applicant on June 8th, 2017 application No. is 2017104292799, entitled " one The priority of the application for a patent for invention of scenedesmus, its cultural method and its application of the kind with high GLA contents ".
Invention content
Inventor is found that one plant of scenedesmus, algae strain can carry out illumination from health at different conditions in the course of the research Long, illumination mixture growth (autotrophy/heterotrophism mixed growth) and pure heterotrophic growth, can accumulate the oil of high-content in growth course Fat, also, ALA accounts for 20% of total lipid content or more, and after adjusting medium component, ALA contents in total fat reach as high as 45%, therefore, algae strain can be used for producing ALA or feed addictive or food additives with high ALA contents.
It finds and studies based on above, the present invention provides a kind of scenedesmus, algae strain is on May 24th, 2017 is preserved in State's Type Tissue Collection, preserving number are：CCTCC NO：M 2017282.
The aliphatic acid of the total fat of scenedesmus of the present invention is mainly made of 16 carbon, oleic acid, linoleic acid and ALA, and in total fat The content of ALA is stablized under optimum culture condition in 35-45%.For example, the fermenting experiment of 3L is carried out, after 120h, culture Dry cell weight can reach 19.48g L-1, total lipid content 12.1%, the content of ALA is that the yield of 42.42%, ALA is in total fat 0.2g L-1d-1.Therefore, algae strain has the potentiality as industrialized production ALA raw materials.
The present invention also provides the cultural method of above-mentioned scenedesmus, the scenedesmus passes through illumination cultivation or Heterotrophic culture.
In one embodiment, the scenedesmus carries out Heterotrophic culture, and the scenedesmus carries out Heterotrophic culture, including uses and add The step of scenedesmus being cultivated added with the basal medium of carbon source and nitrogen source under dark condition.Under dark condition, algae strain Can complete heterotrophism, the speed of growth than under illumination condition faster.
In a preferred embodiment, the basal medium is the aqueous solution comprising following component：KH2PO4, 0.7g L-1；K2HPO4, 0.3g L-1；MgSO47H2O, 0.3g L-1；FeSO47H2O, 3mg L-1；Glycine, 0.1g L-1；vitamin B1, 0.01mg L-1；A5,1ml L-1.Wherein the ingredient of A5 is boric acid (H3BO3) 2.86g/L, tetrahydrate manganese chloride (MnCl2· 4H2O) 1.81g/L, white vitriol (ZnSO4·7H2O) 0.22g/L, Sodium Molybdate Dihydrate (Na2MoO4·2H2O) 0.39g/L, five Brochanite (CuSO4·5H2O) 0.08g/L, CoCL2 6H2O (CoCl2·6H2O)0.01g/L.The basal medium component is only Type and content for the suitable nitrogen source and carbon source of scenedesmus for testing the present invention, are not intended to limit the present invention.People in the art Member can select any desired basal medium to cultivate the scenedesmus of the present invention as needed, for example, BG11 cultures also may be selected Base is as basic culture medium.
In a preferred embodiment, the carbon source is 10-40g L-1Glucose.The additive amount of glucose is to the algae The speed of growth at strain initial stage influences less, still, after culture to 96h, is added to 20-40g L-1In the culture medium of glucose, algae Strain is still within exponential growth so that algae strain can finally reach higher concentration, to improve unit volume culture solution In aliphatic acid yield.
In a preferred embodiment, the nitrogen source is urea, potassium nitrate and/or yeast extract.With urea, nitric acid Any one of potassium and yeast extract or several combinations.Nitrogen source appropriate is conducive to the growth of algae strain.
In a preferred embodiment, the nitrogen source is 1-10g L-1Urea.Compared with other nitrogen sources, urea is more just Preferably.The additive amount of urea is in 1-10g L-1In the range of total lipid content is influenced it is little.But when urea content is in 4-7g L-1 In the range of when, the content of ALA is higher.
In a preferred embodiment, the cultivation temperature of the scenedesmus is 25-35 DEG C.Algae strain is equal at 25-35 DEG C There can be good growth, especially between 30-35 DEG C, faster, state is more preferable for the speed of growth, when temperature is less than 20 DEG C, the life of algae strain Long speed significantly reduces.When in the range of 25-30 DEG C, the content of ALA is higher.
The present invention also provides application of the above-mentioned scenedesmus in preparing ALA.
The present invention also provides effect of the above-mentioned scenedesmus in the feed addictive or food additives for preparing high ALA contents
The present invention also provides the methods for using above-mentioned scenedesmus to be used to prepare aliphatic acid, especially ALA.
In a preferred embodiment, the scenedesmus for being used to prepare aliphatic acid is obtained by above-mentioned cultural method culture It arrives.
Microbial preservation
Scenedesmus of the present invention was preserved in China typical culture collection center (CCTCC) on May 24th, 2017, protected Hiding number is：CCTCC NO：M 2017282.China typical culture collection center deposits the scenedesmus on June 8th, 2017 Activity determination finishes, and result is survival.
Description of the drawings
Fig. 1 is the light micrograph of scenedesmus HSJ296；
Fig. 2 is growth curves of the scenedesmus HSJ296 under different nitrogen sources；
Fig. 3 is growth curves of the scenedesmus HSJ296 under different urea concentrations；
Fig. 4 is total lipid contents of the scenedesmus HSJ296 under different urea concentrations；
Fig. 5 is the growth curves of scenedesmus HSJ296 at different temperatures；
Fig. 6 is the total lipid contents of scenedesmus HSJ296 at different temperatures；
Fig. 7 is growth curves of the scenedesmus HSJ296 under different concentration of glucose；
Fig. 8 is total lipid contents of the scenedesmus HSJ296 under different concentration of glucose；
Fig. 9 is growth curves of the scenedesmus HSJ296 in fermentation tank.
Specific implementation mode
Principles and features of the present invention are described below in conjunction with example, the given examples are served only to explain the present invention, and It is non-to be used to limit the scope of the present invention.
1. Strain selection and preservation
Researcher isolated 232 plants of green algas from the water sample of field acquisition by the method that isolates and purifies, for sieving The higher green alga of ALA contents of complete heterotrophic growth is capable of in choosing.
The green alga that inventor purifies 232 plants of degermings, it is 30g L to be inoculated into equipped with 5ml concentration of glucose-1BG11 examination Guan Zhong, using temperature control shaking table culture, cultivation temperature is set as 30 DEG C, and rotating speed is set as 150rpm, and incubation time is 5 days.Pass through Observation filters out 30 plants of green algas for capableing of complete heterotrophism.Will Heterotrophic culture 30 plants of green algas, be inoculated into be equipped with respectively It is cultivated in the 500ml triangular flasks of 120ml BG11, condition of culture is as previously described.After culture 5 days, algae solution is collected by centrifugation, freezing is dry It is dry to constant weight, weigh 0.1g algaes powder respectively and extract its grease.By its grease esterification, fat in its grease is surveyed using gas-chromatography Fat acid forms and the relative amount of each ingredient.
ALA is filtered out by above method and accounts for the highest algae strain of total lipid content, and when condition of culture is not optimised, ALA accounts for total fat Content is 25.78%.Using optical microphotograph sem observation frustule form, show it with Scenedesmus feature (Fig. 1).It is thin to extract algae Born of the same parents DNA is template, obtains 18S rDNA with 18S rDNA universal PC R primer amplifications, is sequenced, sequence such as SEQ ID NO: Shown in 1.Through sequence alignment, confirms that it belongs to Scenedesmus (Scenedesmus), be named as scenedesmus HSJ296 (Scenedesmus sp.HSJ296)
Inventor on May 24th, 2017 by the algae strain send it is big to No. 299 Wuhan of Wuhan City, Hubei Province Wuchang District Bayi Road Type Tissue Collection (CCTCC) in school carries out microbial preservation, and deposit number is：CCTCC NO：M 2017282。
2. the optimization of scenedesmus HSJ296 condition of culture
Basal medium：KH2PO4, 0.7g L-1；K2HPO4, 0.3g L-1；MgSO47H2O, 0.3gL-1；FeSO47H2O, 3mg L-1；Glycine, 0.1g L-1；vitamin B1, 0.01mg L-1；A5,1ml L-1.The basal medium component is only used for as grid Algae HSJ296 provide some must nutrient, and the optimum carbon source that needs and nitrogen source when cultivating scenedesmus HSJ296 for test, And its concentration, it is not intended to limit the present invention.Those skilled in the art can according to oneself condition and need use other basal mediums Group subassembly.
The optimization of 2.1 nitrogen sources
Under the conditions of 30 DEG C of dark Heterotrophic cultures, when using basal medium shaking flask culture scenedesmus HSJ296,5 kinds are compared The influence of nitrogen source, i.e. urea, potassium nitrate, ammonium nitrate, glycine and yeast extract to algae strain growth.Glucose is dense in culture medium Degree is 6g L-1, the concentration of 5 kinds of nitrogen sources is 1g L-1, shaken cultivation 120h.
The results are shown in Figure 2, compares other nitrogen sources, and urea is most suitable for the growth of scenedesmus HSJ296.Secondly, it is extracted with yeast When object or potassium nitrate are as nitrogen source, scenedesmus HSJ296 well-growns.But when using ammonium nitrate or glycine as nitrogen source, scenedesmus HSJ296 growths are very slow.
In order to further study the influence that urea grows scenedesmus HSJ296, we are provided with 1-10g L-1Four in range A urea concentration gradients carry out algae strain culture.Growth curves of the scenedesmus HSJ296 under different urea concentrations is as shown in figure 3,1- 10g L-1Urea concentration within the scope of, algae strain growth rate gap it is little, urea concentration be 4g L-1When it is slightly good.Total lipid content As shown in figure 4, urea concentration is 1,4,7 and 10g L-1When, total lipid content is respectively 9.83%, 10.93%, 10.33% and 10.78%.Urea concentration is 4g L-1, total lipid content is slightly higher.
Therefore, urea concentration is 4g L-1When, it is most suitable for the culture of scenedesmus HSJ296.
The aliphatic acid composition of the total fat of scenedesmus HSJ296 is as shown in table 1 under different urea concentrations.
The aliphatic acid composition (mol%) of the total fat of scenedesmus HSJ296 under the different urea concentrations of table 1
The optimization of 2.2 cultivation temperatures
Under the conditions of dark Heterotrophic culture, when using basal medium shaking flask culture scenedesmus HSJ296, in basal medium Urea concentration is 4g L-1, concentration of glucose is 20g L-1, have studied influence of the temperature to algae strain growth.We are provided with four Temperature gradient, shaken cultivation 120h.
Scenedesmus HSJ296 growth rates at different temperatures are as shown in figure 5, when cultivation temperature is 30 DEG C, scenedesmus HSJ296 Growth rate it is most fast；In 20 DEG C and 25 DEG C, the growth rate of scenedesmus HSJ296 is all relatively slow；At 35 DEG C, growth state is good It is good.The results show temperature is affected to the growth rate of scenedesmus HSJ296.Total lipid content as shown in fig. 6, temperature is At 30 DEG C, the total lipid content of scenedesmus HSJ296 is 11.43%；When temperature is 20 DEG C, 25 DEG C and 35 DEG C, total lipid content is respectively 10.63%, 10.87% and 10.93%.Temperature is 30 DEG C, and the fat content of the algae is highest.The results show, temperature Influence to the oil and fat accumulation of scenedesmus HSJ296 is little.
Therefore, from the aspect of growth rate and fat content two, 30 DEG C are all the most suitable cultivation temperature of scenedesmus HSJ296.
The aliphatic acid composition of the total fat of scenedesmus HSJ296 is as shown in table 2 under different temperatures.
The aliphatic acid composition (mol%) of the total fat of scenedesmus HSJ296 under 2 different temperatures of table
2.3 sugared concentration optimizations
Glucose is the most common carbon source for maintaining algae strain heterotrophic growth.Initial glucose concentration is being explored to scenedesmus When the influence of HSJ296 biomass accumulations, we set glucose concentration gradient to 10,20,30 and 40g L-1, dark different It supports under condition of culture, utilizes shake flask culture scenedesmus HSJ296.Urea concentration in basal medium is 4g L-1, culture temperature Degree is 30 DEG C.Certainly, glucose is used merely as a kind of example of the carbon source of frustule growth, is not intended to limit the present invention.This Field technology personnel can according to oneself condition and need use other carbon sources.
Growth curves of the scenedesmus HSJ296 under different concentration of glucose is as shown in fig. 7, in 10-40gL-1Initial grape Sugared concentration range, it is little to the growth effect of scenedesmus HSJ296 within 96h.But initial glucose is 10g L-1When, culture is arrived After 96h, algae strain is not grown substantially；And Initial sugar concentration is 20,30 and 40g L-1When, after culture to 96h, algae strain is still in refer to The growth of number phase.
From the above data, when in basal medium initial glucose concentration be 10g L-1When, after culture to 96h, training The glucose supported in base is consumed substantially, and carbon source is lacked in culture medium, and algae strain stops growing.After cultivating 120h, the yield of algae strain Respectively 4.97,9.34,10.4 and 11.02g L-1(not shown).Data above illustrates, under the conditions of Heterotrophic culture, Portugal Grape sugar is affected to micro algae growth.
Accumulation for total fat, as shown in figure 8, when concentration of glucose is in 10-40g L-1Between when, total lipid content is respectively 10.43%, 10.22%, 10.42% and 10.06%, slightly fluctuate.Initial glucose concentration influences the accumulation of the algae oil fat Less.
Total fat aliphatic acid composition of scenedesmus HSJ296 is as shown in table 3 under different concentration of glucose.
The aliphatic acid composition (mol%) of the total fat of scenedesmus HSJ296 under the different concentration of glucose of table 3
According to the above experimental result, in the experiment of subsequent fermented and cultured, we can need according to fermentation in 20-40g L-1Glucose concentration range in the suitable sugared concentration of selection, ferment.
3. fermentation tank culture
Using basal medium Heterotrophic culture scenedesmus HSJ296120h, cultivation temperature is 30 DEG C, and initial glucose concentration is 20g L-1, urea concentration is 4g L-1, rotating speed 200rpm do not control pH.
As shown in figure 9, incubation time, between 52-88h, cell starts exponentially to grow, after 90h, which enters Plateau.In 52h, glucose also begins to quickly be consumed, and at the 76th hour, concentration of glucose dropped to 4g L-1(data are not Display).Culture stops growing to 120h, scenedesmus HSJ296, and dry cell weight reaches 19.48g L-1, total lipid content 12.1%, always The content of ALA is 42.42% in fat.The yield of ALA can reach 1g L-1, i.e. the yield of ALA is 0.2g L-1d-1。
Total fat aliphatic acid composition of the scenedesmus HSJ296 of fermentation tank culture is as shown in table 4.
Total fat aliphatic acid composition (mol%) of the scenedesmus HSJ296 of 4 fermentation tank culture of table
The growth rate of microalgae and the yield of ALA are improved by improveing condition of culture and the training method of microalgae, as a result Show that the most suitable cultivation temperature of scenedesmus HSJ296 is 30 DEG C, optimum nitrogen source is 4g L-1Urea, glucose initial concentration is in 20-40g L-1The growth of the algae is suitable in range.Under optimum culture condition, the aliphatic acid of the total fat of scenedesmus HSJ296 is mainly by 16 carbon (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and ALA compositions, and in total fat the stable content of ALA 35-45% it Between.
The fermenting experiment for carrying out 3L, after 120h, the dry cell weight of scenedesmus HSJ296 can reach 19.48g L-1, total lipid content It is 12.1%, it is 0.2gL that the content of ALA, which is the yield of 42.42%, ALA, in total fat-1d-1.In the future, with fermentation condition into One-step optimization, the content and yield of ALA are expected to be significantly improved.Scenedesmus HSJ296 has to be added as production animal feed microalgae The potentiality for adding agent to supplement ALA and other nutrition, while can also be used as the raw material that extraction prepares ALA.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Sequence table
<110>Inst. of Hydrobiology, Chinese Academy of Sciences
<120>A kind of scenedesmus, its cultural method and its application with high ALA contents
<130> 1
<141> 2018-04-16
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<170> SIPOSequenceListing 1.0
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<213> Scenedesmus HSJ296
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ttacgacttc tccttcctct aggtgggagg gtttaatgaa cttctcggca gacctgaggt 120
gttgccaccc taagctgcca atccgaacac ttcaccagca cacccaatcg gtaggagcga 180
cgggcggtgt gtacaaaggg cagggacgta atcgacgcaa gctgatgact tgcgcctact 240
aggcattcct cgttgaagac taataattgc aataatctat ccccatcacg atgcagtttc 300
aaagattacc cggacctttc ggtcaaggat aggcttgttg aatgcatcag tgtagcgcgc 360
gtgcggccca gaacatctaa gggcatcaca gacctgttat tgcctcatac ttccattgac 420
taaacgccaa tagtccctct aagaagtcag ccagctgcaa aaagcaactg agactattta 480
gcaggctgag gtctcgttcg ttaccggaat caacctgaca aggcaaccca ccaactaaga 540
acggccatgc accaccaccc atagaatcaa gaaagagctc tcaatctgtc aatcctcact 600
atgtctggac ctggtaagtt ttcccgtgtt gagtcaaatt aagccgcagg ctccacgcct 660
ggtggtgccc ttccgtcaat tcctttaagt ttcagccttg cgaccatact ccccccggaa 720
cccaaaaact ttgatttctc ataaggtgct ggcgaagtca ttaaaaaaac attcgccaat 780
ccctagtcgg catcgtttat ggttgagact acgacggtat ctaatcgtct tcgagccccc 840
aactttcgtt cttgattaat gaaaacatcc ttggcaaatg ctttcgcagt agttcgtctt 900
tcataaatcc aagaatttca cctctgacat gaaatacgaa tgcccccgac tgtccctctt 960
atcattactc cggtcctaca gaccaacaag ataggccaga gtcctatcgt gttattccat 1020
gctaaagtat tctggcgtaa gcctgctttg aacactctaa tttactcaaa gtaaccacgt 1080
cgactccgag tcccggacag tgaagcccag aagcccgtcc ccgacagaaa ggaaggccat 1140
agcagtactc accataggcg gaccgctaga acccacccga aatccaacta cgagcttttt 1200
aactgcaaca acttaaatat acgctattgg agctggaatt accgcggctg ctggcaccag 1260
acttgccctc caatggatcc tcgttaaggg atttagattg tactcattcc aattaccaga 1320
cattaaatgc ccgatattgt tatttattgt cactacctcc ccgtatcagg attgggtaat 1380
ttgcgcgcct gctgccttcc ttggatgtgg tagccgtttc tcaggctccc tctccggaat 1440
cgaaccctaa tcctccgtca cccgttacca ccatggtagg cctctatcct accatcgaaa 1500
gttgataggg cagaaatttg aatggaacag cgccggcaca aggccatgcg cttcgtgaag 1560
atatcatgat tcaccgcggg tcggacaaag tccggtcggc cttttatcta atatatacgt 1620
cccttccagg agtcgggatt tacgcacgta ttagctctag aattactacg gttatccgaa 1680
tagtaaggta ccaccaaata aactataact gatttaatga gccattcgca gtttcacagt 1740
ataagcagtt tatacttaga catgcatggc ttaatctttg agacaagcat atgactactg 1800
gcaggatcaa ccaaatctct agaggatccc cgggtaccga gctcgaatc 1849

Claims (10)

1. a kind of scenedesmus, which is characterized in that be preserved in China typical culture collection center, preserving number on May 24th, 2017 For：CCTCC NO：M 2017282.
2. the cultural method of scenedesmus described in claim 1, which is characterized in that carry out illumination cultivation or Heterotrophic culture.
3. cultural method according to claim 2, which is characterized in that the scenedesmus carries out Heterotrophic culture, including uses and add The step of scenedesmus being cultivated added with the basal medium of carbon source and nitrogen source under dark condition.
4. cultural method according to claim 3, which is characterized in that the carbon source is 20-40g L-1Glucose.
5. cultural method according to claim 3, which is characterized in that the nitrogen source is urea, potassium nitrate and yeast extraction Any one of object or several combinations.
6. cultural method according to claim 5, which is characterized in that the nitrogen source is 1-10g L-1Urea.
7. according to the cultural method described in any one of claim 3-6, which is characterized in that the cultivation temperature of the scenedesmus is 25-35℃。
8. application of the scenedesmus described in claim 1 in preparing ALA.
9. application of the scenedesmus described in claim 1 in the feed addictive or food additives for preparing high ALA contents.
10. a kind of method preparing ALA, which is characterized in that extract ALA from scenedesmus described in claim 1.
CN201810337916.4A 2017-06-08 2018-04-16 Scenedesmus with high alpha-linolenic acid content, and culture method and application thereof Expired - Fee Related CN108587913B (en)

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