CN108576061A - a kind of bactericidal composition containing propineb and bacillus subtilis - Google Patents
a kind of bactericidal composition containing propineb and bacillus subtilis Download PDFInfo
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- CN108576061A CN108576061A CN201810786482.6A CN201810786482A CN108576061A CN 108576061 A CN108576061 A CN 108576061A CN 201810786482 A CN201810786482 A CN 201810786482A CN 108576061 A CN108576061 A CN 108576061A
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- China
- Prior art keywords
- bacillus subtilis
- propineb
- bactericidal composition
- downy mildew
- composition containing
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Abstract
The present invention relates to a kind of bactericidal composition containing Propineb and bacillus subtilis, active ingredient is made of Propineb and bacillus subtilis, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound, the weight ratio of Propineb and bacillus subtilis is 5 100:1.Two active ingredient mechanism of action of the present invention are different, synergistic significantly for preventing crop downy mildew, advantageously reduce applications of pesticide amount, reduce pesticide cost.
Description
Technical field
The present invention relates to cultural control technical field, specifically a kind of Bactericidal composite containing Propineb and bacillus subtilis
Object.
Background technology
Propineb, English name：Propineb is a kind of protection type fungicide of quick-acting long residual wide spectrums, sterilization machine
System is not fixed, and site of action is more, the main oxidation for inhibiting pyruvic acid in pathogen body, to vegetables, grape, tobacco and hops etc.
The downy mildew of crop, tomato and the morning of potato, late blight have an excellent protective effect, and to powdery mildew, rust and
The disease of Botrytis also has certain inhibiting effect.
Bacillus subtilis, English name：Bacillus subtilis are a kind of microbial bactericides, by effectively at
Pathogen is killed and controlled to the mode of action for dividing the antibacterial material and site competition, induction of resistance of producing bacillus subtilis life, from
And achieve the purpose that controlling disease.
Downy mildew refers to the plant disease caused by the downy mildew in fungi.In agricultural production, dimethomorph is anti-
The principal item of downy mildew is controlled, therefore long term frequent uses, germ has produced larger resistance, drug effect is caused to reduce, and prevents
It is undesirable to control effect.Therefore, that seeks dimethomorph just seems particularly necessary instead of medicament.
In agricultural production, the farm chemical ingredients mixture using different role mechanism is the important measures of pest and disease damage resistance management
One of, by recipe determination, synergistic effect significantly with that can greatly improve drug effect, reduces drug cost, extends the life of medicament
The period is ordered, is the important means of integrated pest management.Currently, using Propineb and bacillus subtilis as the pesticide of active ingredient
The research that composition is applied to downy mildew yet there are no relevant report.
Invention content
The object of the present invention is to provide a kind of bactericidal composition containing Propineb and bacillus subtilis, for preventing
The prevention of downy mildew has drug effect high, and drug cost is low, the advantages of being conducive to germ resistance management.
The technical solution adopted by the present invention is：A kind of bactericidal composition containing Propineb and bacillus subtilis, effectively at
Point be made of Propineb and bacillus subtilis, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be
The weight ratio of 100% active compound, Propineb and bacillus subtilis is 5-100:1, it is preferred that Propineb and bacillus subtilis
Weight ratio is 20:1 or 40:1.
The weight percent of the bactericidal composition, Propineb is 5-60%, the weight percent of bacillus subtilis
For 0.05-1.0%, i.e., content of the bacillus subtilis in fungicide be 500,000,000 spores/gram~10,000,000,000 spores/gram.
The bactericidal composition is added corresponding adjuvant, is prepared into according to method known in those skilled in the art
Agriculturally acceptable wettable powder, water dispersible granules.
The adjuvant of the bactericidal composition includes filler, dispersant, wetting agent, disintegrant, stabilizer or other has
Stablize in the formulation beneficial to active ingredient and substance that drug effect plays, be all commonly use in pesticidal preparations or allow to use it is various at
Point.
The present invention has the following advantages and significant technique effect：
(1) inventive compound Propineb is different with the mechanism of action of bacillus subtilis, and the two mixture is synergistic
Obviously, drug effect can be greatly improved, Pesticide use amount and drug cost are reduced.
(2) potential resistance risk of the existing germ to Propineb, bacillus subtilis is greatly reduced in composition of the invention,
Be conducive to the resistance management of disease.
Specific implementation mode
Technical scheme of the present invention and technique effect are described further with reference to specific embodiment, institute of the present invention
The percentage stated is weight percentage.
One, indoor toxicity determination test
In order to prevent the germ for agriculturally generating resistance, we have carried out mutual compounding with Propineb, bacillus subtilis
Synergy research.Experiment uses cucumber downy mildew for test object, carries out toxicity test experiment.
1. test method：
1.1 test materials are bacterium of downy mildew of cucumber.Test medicine provides active compound for producer, and it is former to weigh a certain amount of Propineb
Medicine, bacillus subtilis active compound after Propineb, bacillus subtilis enter a certain amount of Tween-80, are directly matched with sterile water dissolution
At mother liquor.On the basis of mother liquor, by trial test as a result, according to Propineb, the different ratio of bacillus subtilis, aqua sterilisa is used
Mother liquor is diluted to appropriate series concentration, prepares dilution of 5 series for examination concentration, each concentration sets 4 or more and repeats.
The cucumber downy mildew leaf adopted back from field is taken back interior by 1.2, is promoted on scab for 24 hours in 21 DEG C or so moisturizing cultures
More spores are formed, are then under sterile washing, being diluted under low power lens 3-5 spore in (10 × 15) each visual field
Preferably (through blood count board test, this concentration quantity up to 3 × 106 to 5 × 106/ML), it and is added in spore suspension
0.25% gelatin with improve spore blade face adherence.
1.3 select growing way prosperous after potting cucumber grows to the 4th true leaf expansion, and growth is more uniform (every for connecing bacterium experiment
Basin is colonized 2 young plants), 10 basins are often handled, are repeated 4 times.Bacterium is first connect, by Fresh spores capsule suspension spray in vacuum side of blade.Per basin
Bacterium solution 5ml is sprayed, (20-22 DEG C) moisturizing (relative humidity is more than 90%) is kept the temperature after inoculation to ensure the invasion of germ.It is trained in moisturizing
It sprays after supporting 24 hours, is designed according to experimental series concentration, routinely constant is sprayed using turntable level pressure metered dose method for each processing
Mist dries.In daily illumination/dark (12/12 hour) after inoculation, temperature is 17-22 DEG C, the condition of 90% or more relative humidity
Lower adjustable hot-house culture.10 basins are often handled, are repeated 4 times.After morbidity is stablized, investigated according to cucumber downy mildew grade scale complete stool
Disease index, and make evaluating drug effect with blank control.
2. statistical analysis technique：
2.1 severity Scaling standards：
0 grade：Blade disease-free spot；
1 grade：Lesion area accounts for 5% or less entire blade area；
3 grades：Lesion area accounts for the 6-10% of entire blade area；
5 grades：Lesion area accounts for the 11-25% of entire blade area；
7 grades：Lesion area accounts for the 26-50% of entire blade area；
9 grades：Lesion area accounts for 50% or more of entire blade area.
2.2 computational methods：
The toxicity index EC of each medicament is calculated according to Sun Yunpei's Method50And the co-toxicity coefficient (CTC values) of mixture, i.e. CTC≤80
For antagonism, 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Co-toxicity coefficient (CTC)=mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI)
Survey toxicity index (ATI)=standard agent EC50/ reagent agent EC50×100
Percentage composition+B the medicament toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
The percentage composition of B in agent
Toxicity test the results are shown in Table 1.
Recipe determination result statistical form of the 1 Propineb bacillus subtilis of table to bacterium of downy mildew of cucumber
As shown in Table 1, Propineb, bacillus subtilis are to the E of cucumber downy mildewC50Respectively 10.79mg/L and
2.53mg/L, when Propineb and bacillus subtilis mixed ratio are in 5-100:When 1, CTC is all higher than 120, has good increasing
Effect acts on, when the weight ratio of Propineb and bacillus subtilis is 20:When 1, co-toxicity coefficient (CTC) is up to 186.7, and the two is mixed
It is the most notable with synergy, there is good application value in agricultural production.
Two, embodiment is prepared
Embodiment 1：10.0% Propineb, 5,000,000,000 CFU/ grams of bacillus subtilis wettable powder
Formula：It is 20 according to the weight ratio of Propineb and bacillus subtilis:1, Propineb 10%, withered grass bud are weighed according to quantity
Spore bacillus 0.5%, wooden sodium sulfonate 2.5% (dispersant), lauryl sodium sulfate 3.2% (wetting agent), white carbon 10% (are filled out
Material), diatomite (filler) complements to 100%.
Above each ingredient is sufficiently mixed in the ratio of formula, it is gloomy to get 10.0% the third after ultra-fine pulverizer disintegrating
The CFU/ grams of bacillus subtilis wettable powder of zinc 5,000,000,000.
Embodiment 2：5.0% Propineb, 500,000,000 CFU/ grams of bacillus subtilis wettable powder
Formula：It is 100 according to the weight ratio of Propineb and bacillus subtilis:1, Propineb 5.0%, withered grass are weighed according to quantity
Bacillus 0.05%, wooden sodium sulfonate 4.5% (dispersant), lauryl sodium sulfate 3.5% (wetting agent), white carbon 10%
(filler), bentonite (filler) complement to 100%.
Above each ingredient is sufficiently mixed in the ratio of formula, to get 5.0% Propineb after ultra-fine pulverizer disintegrating
500000000 CFU/ grams of bacillus subtilis wettable powders.
Embodiment 3：40% Propineb, 5,000,000,000 CFU/ grams of bacillus subtilis water dispersible granules
Formula：It is 80 according to the weight ratio of Propineb and bacillus subtilis:1, Propineb 40%, withered grass bud are weighed according to quantity
Spore bacillus 0.5%, sodium lignin sulfonate 4.5%, polymerization of carboxylic acid sodium 5.0%, methyl naphthalene sulfonate formaldehyde condensate 4.0%, sea
Mosanom 2.5%, white carbon 5.0%, bentonite (filler) complement to 100%.
Above each ingredient is mixed according to the formula uniformly, through air-flow crushing at pulvis, a certain amount of water mixing is added
Material is made in extruding, and 40% Propineb, 5,000,000,000 CFU/ grams of bacillus subtilis water dispersible granules are obtained after dry screening.
Embodiment 4：60% Propineb, 10,000,000,000 CFU/ grams of bacillus subtilis water dispersible granules
Formula：It is 60 according to the weight ratio of Propineb and bacillus subtilis:1, Propineb 60%, withered grass bud are weighed according to quantity
Spore bacillus 1%, calcium lignosulfonate 3.5%, polymerization of carboxylic acid sodium 4.5%, methyl naphthalene sulfonate formaldehyde condensate 3.2%, seaweed
Sour sodium 5.0%, diatomite complements to 100%.
Above each ingredient is mixed according to the formula uniformly, through air-flow crushing at pulvis, a certain amount of water mixing is added
Material is made in extruding, and 60% Propineb, 10,000,000,000 CFU/ grams of bacillus subtilis water dispersible granules are obtained after dry screening.
Three, Field information embodiment
Embodiment 1：Prepare preparation (10.0% Propineb, the 5000000000 CFU/ grams of bacillus subtilis wettable of embodiment 1
Pulvis) prevention cucumber downy mildew field trial
The formula prevents cucumber downy mildew and sprays by 1500 times of dilutions, and 7 days control effects are 94.8% after medicine.20% the third gloomy
Zinc wettable powder is sprayed by 1000 times of dilutions, and 7 days control effects are 81.6% after medicine, 20,000,000,000 spores/gram bacillus subtilis
Wettable powder is sprayed by 800 times of dilutions, and 7 days control effects are 72.3% after medicine.Experiments have shown that：10.0% Propineb 5,000,000,000
CFU/ grams of bacillus subtilis wettable powder has good control effect to cucumber downy mildew, is better than corresponding single dose.
Embodiment 2：Prepare preparation (40% Propineb, the 5000000000 CFU/ grams of bacillus subtilis water-dispersible grain of embodiment 3
Agent) prevention downy mildew of garpe field trial
The formula prevents downy mildew of garpe and sprays by 2000 times of dilutions, and 7 days control effects are 92.5% after medicine.50% the third gloomy
Zinc water dispersible granules suspending agent is sprayed by 1500 times of dilutions, and 7 days control effects are 81.2% after medicine, 100,000,000,000 spores/gram withered grass
Bacillus wettable powder is (purchased in market) to spray by 1500 times of dilutions, and 7 days control effects are 70.4% after medicine.Experiments have shown that 40%
The CFU/ grams of bacillus subtilis water dispersible granules of Propineb 5,000,000,000 have good control effect to downy mildew of garpe, better than corresponding
Single dose.
Claims (4)
1. a kind of bactericidal composition containing Propineb and bacillus subtilis, which is characterized in that active ingredient is by Propineb and withered
Careless bacillus composition, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound, Propineb with
The weight ratio of bacillus subtilis is 5-100:1.
2. bactericidal composition according to claim 1, which is characterized in that the weight ratio of Propineb and bacillus subtilis is
20:1 or 40:1.
3. bactericidal composition according to claim 1 or 2, which is characterized in that the weight percent of Propineb is 5-60%,
The weight percent of bacillus subtilis is 0.05-1.0%.
4. bactericidal composition according to claim 1 is in the application of prevention crop downy mildew.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810786482.6A CN108576061A (en) | 2018-07-17 | 2018-07-17 | a kind of bactericidal composition containing propineb and bacillus subtilis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810786482.6A CN108576061A (en) | 2018-07-17 | 2018-07-17 | a kind of bactericidal composition containing propineb and bacillus subtilis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108576061A true CN108576061A (en) | 2018-09-28 |
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ID=63617881
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810786482.6A Withdrawn CN108576061A (en) | 2018-07-17 | 2018-07-17 | a kind of bactericidal composition containing propineb and bacillus subtilis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108576061A (en) |
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2018
- 2018-07-17 CN CN201810786482.6A patent/CN108576061A/en not_active Withdrawn
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Application publication date: 20180928 |
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