CN108531365A - A kind of method for determining bacteria - Google Patents
A kind of method for determining bacteria Download PDFInfo
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- CN108531365A CN108531365A CN201810339357.0A CN201810339357A CN108531365A CN 108531365 A CN108531365 A CN 108531365A CN 201810339357 A CN201810339357 A CN 201810339357A CN 108531365 A CN108531365 A CN 108531365A
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Abstract
The invention belongs to bacteriologic test technical fields, specifically a kind of method for determining bacteria, the method of inspection uses inspection equipment, the inspection equipment includes incubator, case lid, mounting base, liquid injection pipe, cleaning module and vibration module, incubator is cylindrical shape, and case lid is disc, and case lid is located at the top of incubator, inlet is set on case lid, and inlet is used to that indentifying substance to be added to the inside of incubator；Mounting base is horizontally arranged on culture chamber interior wall；Liquid injection pipe is vertically installed in mounting base, and liquid injection pipe is used to inject indentifying substance to the inside of incubator；Cleaning module is arranged inside liquid injection pipe, cleaning module is for cleaning fluid injection inside pipe wall, it prevents from causing mixing to interfere when injecting different types of indentifying substance to liquid injection pipe, vibration module is for making bacterial solution be sufficiently mixed with indentifying substance, this method avoids different indentifying substances from having an impact Bacteria Identification result by liquid injection pipe and cooperating with each other for cleaning module, improves the accuracy of Bacteria Identification.
Description
Technical field
The invention belongs to bacteriologic test technical field, specifically a kind of method for determining bacteria.
Background technology
Bacterium distributed pole in nature is wide, and quantity is big, and type is more, it can promote the well-being of mankind, can also become cause a disease
Reason.Bacterium can be divided into three classes according to by shape, i.e.,：Coccus, bacillus and spirillum, different types of bacterium is in the daily life of the mankind
Play the role of also being not quite similar in work, requirement of the production process that the mankind have to bacterial species is very stringent, so working out
It is a topic for being worth people to inquire into always that one, which is easily carried out method for determining bacteria at low cost,.
Current common method for determining bacteria, mainly by biochemical method according to the physiological activity feature of bacterium itself come
It identifies bacterium, before identification, bacterium is put into and is cultivated in culture medium, then indentifying substance is added into culture medium, observe it
Color change judges specific bacterium colony type with this, or suspension is made in bacterium colony, according to special identification card and specialized analyzer pair
Suspension is identified, determines bacterium colony type.But in the first identification method, when for identifying various bacteria type,
When injecting indentifying substance into culture medium, remaining a kind of indentifying substance in the equipment of indentifying substance is added and influences whether bacterium
Qualification result, and some qualification process be then by the way of replacing liquid-injection equipment, to ensure the accuracy of qualification result, behaviour
Make cumbersome, determination rates are low.
In consideration of it, a kind of method for determining bacteria of the present invention, by the way that the training in evaluation apparatus inside incubator is arranged
Foster ware holds bacterial solution and inoculum, then is cleaned to the cleaning cloth of fluid injection inside pipe wall by cleaning module, prevents
The qualification result of different indentifying substance mixed influence bacteriums avoids the replacement identification before carrying out second of Bacteria Identification from setting
It is standby, the amount of labour of Bacteria Identification staff is reduced, the accuracy of identification is improved.
Invention content
In order to make up for the deficiencies of the prior art, the present invention proposes a kind of method for determining bacteria, and present invention is mainly used for thin
The identification of strain class.The present invention passes through liquid injection pipe injection identification examination in culture dish into vibration module for being arranged in evaluation apparatus
Agent prevents under the mutual cooperation of liquid injection pipe and cleaning module and causes to mix between different indentifying substances, avoids to bacterium
Qualification result have an impact, reduce Bacteria Identification workload and improve Bacteria Identification accuracy.
The technical solution adopted by the present invention to solve the technical problems is：A kind of method for determining bacteria of the present invention,
The identification method use following evaluation apparatus, the evaluation apparatus include incubator, case lid, mounting base, liquid injection pipe, cleaning module and
Vibration module, the incubator are cylindrical shape, and incubator top side wall opens up toroidal cavity, water is marked in toroidal cavity,
Water can realize that the sealing between case lid and incubator, sealing avoid the microorganism in ambient atmos from passing through between case lid and incubator
Gap enter in the culture dish inside incubator, influence the breeding of bacterium and qualification process in culture dish；The case lid
For disc, case lid is located at the top of incubator, and case lid surrounding is arranged at the top of circular ring shape protrusion and circular ring shape protrusion and incubator
Toroidal cavity contacts, and truncated cone-shaped protrusion is arranged in case lid bottom centre, and the center of truncated cone-shaped protrusion opens up inlet, feed liquor upwards
Mouth is used to that indentifying substance to be added to the inside of incubator；The mounting base is horizontally arranged on culture chamber interior wall；The liquid injection pipe is perpendicular
It is directly mounted in mounting base, liquid injection pipe is cylinder type, the top of liquid injection pipe and the truncated cone-shaped projection contacts of case lid bottom centre, note
Liquid pipe is used to inject indentifying substance to the inside of incubator；The cleaning module is arranged inside liquid injection pipe, and cleaning module is for clear
Fluid injection inside pipe wall is washed, prevents from causing mixing to interfere when injecting different types of indentifying substance to liquid injection pipe；The vibration module is set
It sets in the bottom of incubator, vibration module is for making indentifying substance and bacterial solution be sufficiently mixed.When work, by inlet to
Indentifying substance is added inside incubator, indentifying substance passes through the vibration module of arrival incubator bottom after liquid injection pipe, vibration module
So that indentifying substance and bacterial solution is sufficiently mixed, bacterial species are differentiated；
The method for determining bacteria includes the following steps：
Step 1：Sterilizing first is carried out to incubator and liquid injection pipe with ultraviolet light, prevents incubator and liquid injection pipe itself
Qualification process of the existing bacteria effect to bacterium；
Step 2：Inoculum and bacterial solution are put into the culture dish inside the incubator in step 1, covered
Case lid, bacterium breed in inoculum；
Step 3：After covering the case lid in step 2, to the toroidal cavity of incubator top side wall in water filling, case lid
Between incubator be in sealing state, prevent the microorganism of ambient atmos enter in incubator influence Bacteria Culture process with
And qualification result；
Step 4：Motor is opened, the rotation axis band moving cam inside the incubator in step 3 rotates, to drive culture
Ware shakes, and the inoculum and bacterial solution in culture dish are sufficiently mixed, and improve the reproduction speed of bacterium；
Step 5：Negative pressure pump is opened, negative pressure pump drives sucker that cleaning cloth is made to be close to fluid injection inside pipe wall, by step 4
The inlet at the case lid center above incubator injects indentifying substance into culture dish, cam to indentifying substance and bacterial solution into
Row vibration mixing, the color change that is mixed with indentifying substance according to inoculum and bacterial solution identify bacterial species；
Step 6：Negative pressure pump is closed, cleaning cloth is detached from fluid injection inside pipe wall, and unlatching control switch makes the fluid injection in step 5
Driving wheel inside pipe drives driven wheel rotation, to which the cleaning cloth of fluid injection inside pipe wall to be brought into rinse bath, in rinse bath
Cleaning solution remaining indentifying substance on cleaning cloth is cleaned, avoid causing between different indentifying substances mixing to bacterium
Identification interferes；
Step 7：Cleaning cloth after being washed comes back to the note in step 6 under the drive of driving wheel and driven wheel
After in the inner wall of liquid pipe, then another indentifying substance is added into culture dish by the inlet of case lid, vibration module is to mirror
Determine reagent and bacterial solution is mixed, further according to the artificial identification bacterial species of solution variation in culture dish, avoids difference
Indentifying substance between mix qualification result impacted.
The cleaning module includes driving wheel, driven wheel, cleans cloth, sucker and negative pressure pump, in the tube wall of the liquid injection pipe
Portion symmetrically opens up rinse bath, and rinse bath is arc empty slot, and rinse bath is symmetrically opened for holding cleaning solution on the inside of the tube wall of liquid injection pipe
If No.1 groove and No. two grooves, No.1 groove is located at the centre of two rinse bath one end, and No. two grooves are located at two rinse baths
The centre of the other end；The driving wheel is mounted in No.1 groove；The driven wheel is mounted in No. two grooves, driving wheel and from
Driving wheel respectively setting two；The cleaning cloth is sleeved on driving wheel and driven wheel, and cleaning cloth is arranged two, and cleaning cloth is elastical cloth,
Cleaning cloth is made of waterproof material, and waterproof material can extend the service life of cleaning cloth, easy to use, and cleaning cloth passes through rinse bath
With the inner wall of liquid injection pipe；No. three grooves symmetrically are opened up on the inside of the tube wall of the liquid injection pipe, No. three grooves are located at No.1 groove and two
The centre of number groove；The sucker and negative pressure pump are mounted in No. three grooves, negative pressure pump for drive sucker suction clean cloth from
And cleaning cloth is made to be close to the inner wall of liquid injection pipe.When work, negative pressure pump is opened, negative pressure pump drives sucker to be adsorbed on fluid injection by cloth is cleaned
On the inner wall of pipe, indentifying substance is added from inlet to inside incubator, when needing replacing indentifying substance, closes negative pressure
Pump, the inner wall for cleaning cloth and liquid injection pipe are detached from, and being then turned on control switch makes driving wheel drive driven wheel rotation, driving wheel and driven
Wheel drives cleaning cloth rotation, enters in rinse bath after cleaning cloth adherency indentifying substance, cleaning solution in rinse bath to cleaning cloth into
Row cleaning, avoids causing cross-infection between different reagents.
The vibration module includes support spring, No.1 bar, placement rack, culture dish, rotation axis, cam, the support bullet
Spring and No.1 bar are connected to incubator bottom vertically；One side of the bottom of the placement rack is connect with support spring, placement rack
The another side of bottom is hinged with No.1 bar, and placement rack is cuboid, and No. four grooves are opened up at the top of placement rack；The culture dish setting
In No. four grooves, culture dish is uncovered rectangular box, and culture dish internal side wall opens up No. five grooves, No. five grooves and culture
Ware bottom is adjacent；The rotation axis is horizontally set on inside incubator, and the both ends of rotation axis are rotatably connected on incubator respectively
On front and rear wall；On the rotating shaft, cam is contacted with placement rack bottom, and cam is rotated for making placement rack for the cam installation
It shakes.Dynamic by motor-driven rotation shaft rotation when work, rotation axis rotation band moving cam rotates, and cam rotation drives placement rack
It is rocked up and down with culture dish, culture dish, which rocks, makes the bacterial solution inside culture dish be sufficiently mixed with indentifying substance, accelerates bacterium
Identification process.
The both sides side wall of the rinse bath is staggered arc chute, and be slidably installed baffle in arc chute, keeps out
Block is that the side of cuboid and baffle is arc, and the baffle of interlaced arrangement is squeezed into S types by cloth is cleaned.When work, keep out
Remaining indentifying substance on arc one end scraped cleaning cloth of block, improves cleaning efficiency of the cleaning cloth in cleaning solution.
Elastic gum is arranged in the culture dish inner bottom；Installation is rolled in the cavity that the elastic gum is constituted with culture dish bottom plate
Column, roller outer ring are wave-like, and cylindrical protrusions are arranged in roller both ends, and cylindrical protrusions are located in No. five grooves, roller rolling
Employ in make elastic gum generate wave.When work, placement rack sliding support spring and No.1 bar supporting role under on have a down dip
Tiltedly, roller is rolled in culture dish bottom, and to make elastic gum rise and fall, bacterial solution, inoculum and indentifying substance fill
Divide mixing, improves the determination rates of bacterium.
The baffle side opens up No.1 through-hole, No.1 rotary shaft is arranged inside No.1 through-hole, No.1 rotary shaft passes through
Holder is mounted on the side wall of No.1 through-hole；It is rotatablely connected helical blade in the No.1 rotary shaft.When work, driving wheel, from
Wheel rotation drive cleaning cloth rotation, cleaning cloth drive baffle to be slided on arc chute, and helical blade, which rotates, to be made clearly
Cleaning solution in washing trough generates shaking, improves the cleaning efficiency of cleaning cloth.
Baffle arc side opens up No. six grooves, and No. two rotary shafts are arranged in No. six grooves, and No. two rotary shafts are hung down
Directly in the side of No. six grooves, the side of No. two rotary shafts and No. six grooves is rotation connection, and flabellum is connected in No. two rotary shafts,
Flabellum is contacted with cleaning cloth.When work, when cleaning cloth moves, No. two rotary shafts drive flabellums rotation, flabellum rotation to improve clear
The mobility of washing lotion so that cleaning cloth cleans more abundant.
The beneficial effects of the invention are as follows：
1. a kind of method for determining bacteria of the present invention, the present invention include Step 1: Step 2: Step 3: Step 4:
Step 5: step 6 and step 7, Step 1: step 2 and step 3 belong to the preparation before Bacteria Culture, step 4 is used
In Bacteria Culture, step 5 is for identifying that bacterial species, step 6 and step 7 are used to prevent to remain in last liquid injection pipe
Indentifying substance influence the qualification result of next bacterium, this method is easily operated, in the case where being changed without reservoir container with not
Same indentifying substance differentiates different bacteriums, avoids causing mixing to interfere between indentifying substance, mitigates the identification of bacterium
Workload improves the determination rates and accuracy of bacterium.
2. a kind of method for determining bacteria of the present invention, the evaluation apparatus used in of the invention, which passes through, is arranged vibration module
Support spring and cam to the culture dish on placement rack carry out vibration rock, make the bacterial solution in culture dish and indentifying substance
What is mixed is more abundant, so that the qualification result of bacterium is become apparent from, reduces the identification difficulty of bacterium.
3. a kind of method for determining bacteria of the present invention, the evaluation apparatus used in of the invention, which passes through, is arranged incubator top
The circular ring shape protrusion of the toroidal cavity in portion and case lid surrounding, causes to seal, prevents ambient atmos from entering training to incubator inside
It supports and Bacteria Identification is interfered in ware, improve the accuracy of Bacteria Identification in culture dish.
4. a kind of method for determining bacteria of the present invention, the evaluation apparatus used in of the invention, which passes through, is arranged arc chute
Interior baffle is squeezed into S types by cloth is cleaned, and is greatly improved the cleaning efficiency of cleaning cloth, avoids mixed between different reagents
It closes, improves determination rates.
Description of the drawings
The invention will be further described below in conjunction with the accompanying drawings.
Fig. 1 is the operational flowchart of the present invention；
Fig. 2 is the structural schematic diagram of evaluation apparatus；
Fig. 3 is A-A sectional views in Fig. 2；
Fig. 4 is C-C sectional views in Fig. 2；
Fig. 5 is the structural schematic diagram of stopper in evaluation apparatus；
Fig. 6 is B-B sectional views in Fig. 5；
In figure：Incubator 1, toroidal cavity 11, case lid 2, circular ring shape protrusion 21, truncated cone-shaped protrusion 22, inlet 23, peace
Fill seat 3, liquid injection pipe 4, rinse bath 41, arc chute 411,42, No. two grooves 43 of No.1 groove, cleaning module 5, driving wheel 51,
Driven wheel 52, cleaning cloth 53, sucker 54, vibration module 6, support spring 61, No.1 bar 66, placement rack 62, culture dish 63, five
Groove 631, rotation axis 64, cam 65, baffle 7, elastic gum 8, roller 9, cylindrical protrusions 91, No.1 rotary shaft 10, holder
11,12, No. two rotary shafts 13, flabellums 14 of helical blade.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific implementation mode is closed, the present invention is further explained.
As shown in Figures 1 to 6, a kind of method for determining bacteria of the present invention, the identification method are set using following identification
Standby, which includes incubator 1, case lid 2, mounting base 3, liquid injection pipe 4, cleaning module 5 and vibration module 6, the culture
Case 1 is cylindrical shape, and 1 top side wall of incubator opens up toroidal cavity 11, water is marked in toroidal cavity 11, and water can realize case lid
Sealing between 2 and incubator 1, sealing avoid microorganism in ambient atmos by the gap between case lid 2 and incubator 1 into
Enter into the culture dish 63 inside incubator 1, influences the breeding of bacterium and qualification process in culture dish 63；The case lid 2 is
Disc, case lid 2 are located at 1 top of incubator, 2 surrounding of case lid setting circular ring shape protrusion 21 and circular ring shape protrusion 21 and incubator 1
The toroidal cavity 11 at top contacts, and truncated cone-shaped protrusion 22 is arranged in 2 bottom centre of case lid, and the center of truncated cone-shaped protrusion 22 is opened upwards
If inlet 23, inlet 23 is used to that indentifying substance to be added to the inside of incubator 1；The mounting base 3 is horizontally arranged at incubator 1
On inner wall；The liquid injection pipe 4 is vertically installed in mounting base 3, and liquid injection pipe 4 is cylinder type, top and 2 bottom of case lid of liquid injection pipe 4
The truncated cone-shaped protrusion 22 at portion center contacts, and liquid injection pipe 4 is used to inject indentifying substance to the inside of incubator 1；The cleaning module 5 is set
It sets inside liquid injection pipe 4, cleaning module 5 prevents from injecting different types of identification to liquid injection pipe 4 for cleaning 4 inner wall of liquid injection pipe
Mixing is caused to interfere when reagent；The vibration module 6 is arranged in the bottom of incubator 1, vibration module 6 for make indentifying substance and
Bacterial solution is sufficiently mixed.When work, by inlet 23 to indentifying substance is added inside incubator 1, indentifying substance passes through note
The vibration module 6 of 1 bottom of incubator is reached after liquid pipe 4, vibration module 6 makes indentifying substance and bacterial solution be sufficiently mixed, to thin
Strain class is differentiated；
The method for determining bacteria includes the following steps：
Step 1：Sterilizing first is carried out to incubator 1 and liquid injection pipe 4 with ultraviolet light, prevents incubator 1 and liquid injection pipe 4
Qualification process of existing bacteria effect to bacterium itself；
Step 2：Inoculum and bacterial solution are put into the culture dish 63 inside the incubator 1 in step 1, covered
Good case lid 2, bacterium breeds in inoculum；
Step 3：After covering the case lid 2 in step 2, the 11 inner water filling of toroidal cavity to 1 top side wall of incubator,
Sealing state is between case lid 2 and incubator 1, preventing the microorganism of ambient atmos from entering in incubator 1 influences bacterium training
The process of supporting and qualification result；
Step 4：Motor is opened, the rotation axis 64 inside incubator 1 in step 3 is rotated with moving cam 65, to band
Dynamic culture dish 63 shakes, and the inoculum and bacterial solution in culture dish 63 are sufficiently mixed, and improve the reproduction speed of bacterium；
Step 5：Negative pressure pump 55 is opened, negative pressure pump 55 drives sucker 54 that cleaning cloth 53 is made to be close to 4 inner wall of liquid injection pipe, passes through
The inlet 23 at 2 center of case lid of 1 top of incubator in step 4 injects indentifying substance into culture dish 63, and cam 65 is to mirror
Determine reagent and bacterial solution carries out vibration mixing, the color change mixed with indentifying substance according to inoculum and bacterial solution
To identify bacterial species；
Step 6：Negative pressure pump 55 is closed, cleaning cloth 53 is detached from 4 inner wall of liquid injection pipe, and opening control switch makes in step 5
Liquid injection pipe 4 inside driving wheel 51 drive driven wheel 52 to rotate, to which the cleaning cloth 53 of 4 inner wall of liquid injection pipe is brought into cleaning
In slot 41, the cleaning solution in rinse bath 41 cleans remaining indentifying substance on cleaning cloth 53, and different identifications is avoided to try
Mixing is caused to interfere Bacteria Identification between agent；
Step 7：Cleaning cloth 53 after being washed comes back to step 6 under the drive of driving wheel 51 and driven wheel 52
In liquid injection pipe 4 inner wall in after, then another indentifying substance is added into culture dish 63 by the inlet 23 of case lid 2,
Vibration module 6 mixes indentifying substance and bacterial solution, further according to the artificial identification bacterium of solution variation in culture dish 63
Type, avoids to mix between different indentifying substances and is impacted to qualification result.
The cleaning module 5 includes driving wheel 51, driven wheel 52, cleaning cloth 53, sucker 54 and negative pressure pump 55, the fluid injection
The tube wall internal symmetry of pipe 4 opens up rinse bath 41, and rinse bath 41 is arc empty slot, and rinse bath 41 is for holding cleaning solution, fluid injection
Symmetrically open up No.1 groove 42 and No. two grooves 43 on the inside of the tube wall of pipe 4, No.1 groove 42 is positioned at two 41 one end of rinse bath
Centre, No. two grooves 43 are located at the centre of two 41 other ends of rinse bath；The driving wheel 51 is mounted in No.1 groove 42；Institute
Driven wheel 52 is stated in No. two grooves 43, driving wheel 51 and the respectively setting two of driven wheel 52；The cleaning cloth 53 is sleeved on master
On driving wheel 51 and driven wheel 52, cleaning cloth 53 is arranged two, and cleaning cloth 53 is elastical cloth, and cleaning cloth 53 is made of waterproof material,
Waterproof material can extend the service life of cleaning cloth 53, easy to use, and cleaning cloth 53 passes through the interior of rinse bath 41 and liquid injection pipe 4
Wall；Symmetrically open up No. three grooves on the inside of the tube wall of the liquid injection pipe 4, No. three grooves are located at No.1 groove 42 and No. two grooves 43
It is intermediate；The sucker 54 and negative pressure pump 55 are mounted in No. three grooves, and negative pressure pump 55 is for driving the absorption cleaning cloth 53 of sucker 54
To make cleaning cloth 53 be close to the inner wall of liquid injection pipe 4.When work, negative pressure pump 55 is opened, negative pressure pump 55 drives sucker 54 that will clean
Cloth 53 is adsorbed on the inner wall of liquid injection pipe 4, and indentifying substance is added from inlet 23 to inside incubator 1, when needing replacing mirror
When determining reagent, negative pressure pump 55 is closed, cleaning cloth 53 and the inner wall of liquid injection pipe 4 are detached from, and being then turned on control switch makes 51 band of driving wheel
Dynamic driven wheel 52 rotates, and driving wheel 51 and driven wheel 52 drive cleaning cloth 53 to rotate, and cleaning cloth 53 enters after adhering to indentifying substance
In rinse bath 41, the cleaning solution in rinse bath 41 cleans cleaning cloth 53, avoids causing cross-infection between different reagents.
The vibration module 6 includes support spring 61, No.1 bar 66, placement rack 62, culture dish 63, rotation axis 64, cam
65, the support spring 61 and No.1 bar 66 are connected to 1 bottom of incubator vertically；One side of the bottom of the placement rack 62 and branch
It supports spring 61 to connect, the another side of the bottom of placement rack 62 and No.1 bar 66 are hinged, and placement rack 62 is cuboid, and placement rack 62 pushes up
Portion opens up No. four grooves；The culture dish 63 is arranged in No. four grooves, and culture dish 63 is uncovered rectangular box, culture dish 63
Internal side wall opens up No. five grooves 631, and No. five grooves 631 are adjacent with 63 bottom of culture dish；The rotation axis 64 is horizontally set on
Inside incubator 1, the both ends of rotation axis 64 are rotatably connected on respectively on the front and rear wall of incubator 1；The cam 65, which is mounted on, to be turned
On moving axis 64, cam 65 is contacted with 62 bottom of placement rack, and cam 65 is rotated for making placement rack 62 shake.When work, lead to
The rotation of motor-driven rotation axis 64 is crossed, the rotation band moving cam 65 of rotation axis 64 rotates, and the rotation of cam 65 drives placement rack 62 and training
It supports about 63 ware to rock, culture dish 63, which rocks, makes the bacterial solution inside culture dish 63 be sufficiently mixed with indentifying substance, accelerates thin
The identification process of bacterium.
The both sides side wall of the rinse bath 41 is staggered arc chute 411, is slidably installed and keeps out in arc chute 411
Block 7, baffle 7 are that the side of cuboid and baffle 7 is arc, and the baffle 7 of interlaced arrangement is squeezed into S by cloth 53 is cleaned
Type.When work, remaining indentifying substance on arc one end scraped cleaning cloth 53 of baffle 7 improves cleaning cloth 53 and is cleaning
Cleaning efficiency in liquid.
Elastic gum 8 is arranged in 63 inner bottom of the culture dish；In the cavity that the elastic gum 8 is constituted with 63 bottom plate of culture dish
Roller 9 is installed, 9 outer ring of roller is wave-like, and cylindrical protrusions 91 are arranged in 9 both ends of roller, and cylindrical protrusions 91 are located at No. five
In groove 631, roller 9 is rolled for making elastic gum 8 generate wave.When work, placement rack 62 is in sliding support spring 61 and one
Tilted upward under the supporting role of number bar 66, roller 9 is rolled in 63 bottom of culture dish, to make elastic gum 8 rise and fall, bacterium
Bacterium solution, inoculum and indentifying substance are sufficiently mixed, and improve the determination rates of bacterium.
7 side of the baffle opens up No.1 through-hole, and No.1 rotary shaft 10, No.1 rotary shaft 10 is arranged inside No.1 through-hole
It is mounted on by holder 11 on the side wall of No.1 through-hole；Helical blade 12 is rotatablely connected in the No.1 rotary shaft 10.When work,
Driving wheel 51, the rotation of driven wheel 52 drive cleaning cloth 53 to rotate, and cleaning cloth 53 drives baffle 7 to be slided on arc chute 411,
Helical blade 12, which rotates, makes the cleaning solution in rinse bath 411 generate shaking, improves the cleaning efficiency of cleaning cloth 53.
7 arc side of the baffle opens up No. six grooves, and No. two rotary shafts 13, No. two rotary shafts are arranged in No. six grooves
13 perpendicular to the side of No. six grooves, and the side of No. two rotary shafts 13 and No. six grooves is rotation connection, in No. two rotary shafts 13
Flabellum 14 is connected, flabellum 14 is contacted with cleaning cloth 53.When work, when cleaning cloth 53 moves, No. two rotary shafts 13 drive flabellum 14
Rotation, flabellum 14 rotate the mobility for improving cleaning solution so that cleaning cloth 53 cleans more abundant.
When work, first bacterial solution and culture solution are put into culture dish 63, cover case lid 2, injects water into 1 side of incubator
In toroidal cavity 11 at the top of wall, the microorganism in ambient atmos is avoided to enter by the gap between case lid 2 and incubator 1
In culture dish 63 inside to incubator 1, the identification process of bacterium in culture dish 63 is influenced.
Opening motor makes rotation axis 64 rotate, and the rotation band moving cam 65 of rotation axis 64 rotates, and the rotation of cam 65, which drives, places
Seat 62 and about 63 culture dish rock, and to make bacterial solution culture dish 63 inside and indentifying substance be sufficiently mixed, quickening is carefully
The identification speed of bacterium.
Open negative pressure pump 55, negative pressure pump 55 drive sucker 54 by clean cloth 53 be adsorbed on the inner wall of liquid injection pipe 4, by into
Indentifying substance is added into incubator 1 for liquid mouth 23, and indentifying substance reaches culture dish 63 after the cleaning cloth 53 of 4 inner wall of liquid injection pipe
In, the bacterium in culture dish 63 meets indentifying substance and changes, and identifies that certain bacterium whether there is in culture dish with this.
When identifying second of bacterial species, negative pressure pump 55 is closed, so that cleaning cloth 53 and the inner wall of liquid injection pipe 4 is detached from, opens
Opening control switch makes driving wheel 51 that driven wheel 52 be driven to rotate, and driving wheel 51 and driven wheel 52 drive cleaning cloth 53 to rotate, cleaning
Cloth 53 enters in rinse bath 41, and the cleaning solution in rinse bath 41 cleans remaining indentifying substance on cleaning cloth 53, prevents
Cross-infection is caused between different indentifying substances.
Cleaning cloth 53 after being washed comes back to 4 inner wall of liquid injection pipe under the drive of driving wheel 51 and driven wheel 52,
Second of indentifying substance is added into culture dish 63 by inlet 23 again, other bacterium kinds being further continued in identification culture dish 63
Class reduces cleaning link, improves the determination rates of bacterium and the accuracy of identification.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the originals of the present invention
Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle
It is fixed.
Claims (7)
1. a kind of method for determining bacteria, it is characterised in that：It includes training that the identification method, which uses following evaluation apparatus, the evaluation apparatus,
Supporting case (1), case lid (2), mounting base (3), liquid injection pipe (4), cleaning module (5) and vibration module (6), the incubator (1) is
Cylindrical shape, incubator (1) top open up toroidal cavity (11)；The case lid (2) is disc, and case lid (2) is located at incubator
(1) circular ring shape protrusion (21) is arranged in top, case lid (2) surrounding, and circular ring shape protrusion (21) and the circular ring shape at the top of incubator (1) are recessed
Slot (11) contacts, and truncated cone-shaped protrusion (22) is arranged in case lid (2) bottom centre, and the center of truncated cone-shaped protrusion (22) opens up feed liquor upwards
Mouth (23), inlet (23) are used to that indentifying substance to be added to the inside of incubator (1)；The mounting base (3) is horizontally arranged at culture
On case (1) inner wall；The liquid injection pipe (4) is vertically installed in mounting base (3), and liquid injection pipe (4) is cylinder type, liquid injection pipe (4)
Top is contacted with the truncated cone-shaped of case lid (2) bottom centre raised (22), and liquid injection pipe (4) is used to that mirror to be added to the inside of incubator (1)
Determine reagent；Cleaning module (5) setting is internal in liquid injection pipe (4), and cleaning module (5) is for cleaning liquid injection pipe (4) inner wall；Institute
Vibration module (6) setting is stated in the bottom of incubator (1), vibration module (6) is for keeping indentifying substance and bacterial solution fully mixed
It closes；
The method for determining bacteria includes the following steps：
Step 1：Sterilizing first is carried out to incubator (1) and liquid injection pipe (4) with ultraviolet light；
Step 2：In the culture dish for incubator (1) inside that inoculum and bacterial solution are put into step 1, case is covered
It covers (2)；
Step 3：After covering the case lid (2) in step 2, inner noted to the toroidal cavity (11) of incubator (1) top side wall
Water allows and is in sealing state between case lid (2) and incubator (1)；
Step 4：Motor is opened, the internal rotation axis band moving cam of the incubator (1) in step 3 rotates, to drive culture
Ware shakes；
Step 5：Negative pressure pump is opened, negative pressure pump drives sucker that cleaning cloth is made to be close to liquid injection pipe (4) inner wall, by step 4
The inlet (23) at case lid (2) center above incubator (1) injects indentifying substance into incubator (1), and vibration module (6) is right
Indentifying substance and bacterial solution are mixed；
Step 6：Negative pressure pump is closed, opening control and switching makes the driving wheel in the liquid injection pipe (4) in step 5 drive driven rotation
It is dynamic, it is cleaned to which the cleaning cloth of liquid injection pipe (4) inner wall to be brought into rinse bath；
Step 7：Cleaning cloth after being washed comes back to the liquid injection pipe in step 6 under the drive of driving wheel and driven wheel
(4) after in inner wall, then another indentifying substance is added into culture dish by the inlets (21) of case lid (2), vibrates mould
Block (6) mixes to complete the identification to other bacterial species indentifying substance and bacterial solution.
2. a kind of method for determining bacteria according to claim 1, it is characterised in that：The cleaning module (5) includes actively
Take turns (51), driven wheel (52), cleaning cloth (53), sucker (54) and negative pressure pump (55), the tube wall internal symmetry of the liquid injection pipe (4)
Rinse bath (41) is opened up, rinse bath (41) is arc empty slot, and rinse bath (41) is for holding cleaning solution, the tube wall of liquid injection pipe (4)
Inside symmetrically opens up No.1 groove (42) and No. two grooves (43), and No.1 groove (42) is located in two rinse bath (41) one end
Between, No. two grooves (43) are located at the centre of two rinse bath (41) other ends；The driving wheel (51) is mounted on No.1 groove
(42) in；The driven wheel (52) is mounted in No. two grooves (43), driving wheel (51) and driven wheel (52) respectively setting two；Institute
It states cleaning cloth (53) to be sleeved on driving wheel (51) and driven wheel (52), cleaning cloth (53) is arranged two, and cleaning cloth (53) is elasticity
Cloth, cleaning cloth (53) are made of waterproof material, and cleaning cloth (53) passes through the inner wall of rinse bath (41) and liquid injection pipe (4)；The note
No. three grooves are symmetrically opened up on the inside of the tube wall of liquid pipe (4), No. three grooves are located in No.1 groove (42) and No. two grooves (43)
Between；The sucker (54) and negative pressure pump (55) are mounted in No. three grooves, and negative pressure pump (55) is for driving sucker (54) that will clean
Cloth (53) is adsorbed on the inner wall of liquid injection pipe (4).
3. a kind of method for determining bacteria according to claim 1, it is characterised in that：The vibration module (6) includes support
Spring (61), No.1 bar (66), placement rack (62), culture dish (63), rotation axis (64), cam (65), the support spring
(61) and No.1 bar (66) is connected to incubator (1) bottom vertically；One side of the bottom of the placement rack (62) and support spring
(61) it connects, the another side of the bottom of placement rack (62) and No.1 bar (66) are hinged, and placement rack (62) is cuboid, placement rack
(62) top opens up No. four grooves；The culture dish (63) is arranged in No. four grooves, and culture dish (63) is uncovered rectangle case
Body, culture dish (63) internal side wall open up No. five grooves (631), and No. five grooves (631) are adjacent with culture dish (63) bottom；It is described
Rotation axis (64) is horizontally set on that incubator (1) is internal, before the both ends of rotation axis (64) are rotatably connected on incubator (1) respectively
On rear wall；The cam (65) is mounted in rotation axis (64), and cam (65) is contacted with placement rack (62) bottom, and cam (65) turns
It employs in making placement rack (62) shake.
4. a kind of method for determining bacteria according to claim 2, it is characterised in that：The both sides side wall of the rinse bath (41)
Be staggered arc chute (411), and be slidably installed in arc chute (411) baffle (7), baffle (7) be cuboid and
The side of baffle (7) is arc, and the baffle (7) of interlaced arrangement will clean cloth (53) and be squeezed into S types.
5. a kind of method for determining bacteria according to claim 3, it is characterised in that：Culture dish (63) inner bottom is set
Flexible glue (8)；Installation roller (9), roller (9) outer ring in the cavity that the elastic gum (8) is constituted with culture dish (63) bottom plate
For wave-like, cylindrical protrusions (91) are arranged in roller (9) both ends, and cylindrical protrusions (91) are located in No. five grooves (631), rolling
Column (9) is rolled for making elastic gum (8) generate wave.
6. a kind of method for determining bacteria according to claim 4, it is characterised in that：Baffle (7) side opens up one
Number through-hole, setting No.1 rotary shaft (10) inside No.1 through-hole, No.1 rotary shaft (10) are mounted on one number service by holder (11)
On the side wall in hole；Helical blade (12) is rotatablely connected on the No.1 rotary shaft (10).
7. a kind of method for determining bacteria according to claim 4, it is characterised in that：It opens baffle (7) the arc side
If No. six grooves, No. two rotary shafts (13) are set in No. six grooves, No. two rotary shafts (13) are perpendicular to the side of No. six grooves, and two
The side of number rotary shaft (13) and No. six grooves is rotation connection, connection flabellum (14) on No. two rotary shafts (13), flabellum (14)
It is contacted with cleaning cloth (53).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810339357.0A CN108531365A (en) | 2018-04-16 | 2018-04-16 | A kind of method for determining bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810339357.0A CN108531365A (en) | 2018-04-16 | 2018-04-16 | A kind of method for determining bacteria |
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| Publication Number | Publication Date |
|---|---|
| CN108531365A true CN108531365A (en) | 2018-09-14 |
Family
ID=63480881
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810339357.0A Withdrawn CN108531365A (en) | 2018-04-16 | 2018-04-16 | A kind of method for determining bacteria |
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| Country | Link |
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| CN (1) | CN108531365A (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100291619A1 (en) * | 2009-05-15 | 2010-11-18 | Biomerieux, Inc. | Combined detection instrument for culture specimen containers and instrument for identification and/or characterization of a microbial agent in a sample |
| CN205710752U (en) * | 2016-06-26 | 2016-11-23 | 周赞虎 | Micro biological Tests device |
| CN106563380A (en) * | 2016-11-11 | 2017-04-19 | 陈叶青 | Kit with stirring function |
| CN207091409U (en) * | 2017-08-15 | 2018-03-13 | 内蒙古科技大学 | A kind of microorganism species step sizing device |
| CN107841453A (en) * | 2017-11-02 | 2018-03-27 | 广州禾信仪器股份有限公司 | Microorganism-collecting device and collection detection method |
-
2018
- 2018-04-16 CN CN201810339357.0A patent/CN108531365A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100291619A1 (en) * | 2009-05-15 | 2010-11-18 | Biomerieux, Inc. | Combined detection instrument for culture specimen containers and instrument for identification and/or characterization of a microbial agent in a sample |
| CN205710752U (en) * | 2016-06-26 | 2016-11-23 | 周赞虎 | Micro biological Tests device |
| CN106563380A (en) * | 2016-11-11 | 2017-04-19 | 陈叶青 | Kit with stirring function |
| CN207091409U (en) * | 2017-08-15 | 2018-03-13 | 内蒙古科技大学 | A kind of microorganism species step sizing device |
| CN107841453A (en) * | 2017-11-02 | 2018-03-27 | 广州禾信仪器股份有限公司 | Microorganism-collecting device and collection detection method |
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Application publication date: 20180914 |