CN108514093A - A kind of multifunctional food composition of auxiliary hyperglycemic and preparation method thereof - Google Patents

A kind of multifunctional food composition of auxiliary hyperglycemic and preparation method thereof Download PDF

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CN108514093A
CN108514093A CN201810448001.0A CN201810448001A CN108514093A CN 108514093 A CN108514093 A CN 108514093A CN 201810448001 A CN201810448001 A CN 201810448001A CN 108514093 A CN108514093 A CN 108514093A
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parts
group
auxiliary hyperglycemic
food composition
model
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CN108514093B (en
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魏敏杰
宫世强
孟贺
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China Medical University
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China Medical University
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
    • A23L25/30—Mashed or comminuted products, e.g. pulp, pastes, meal, powders; Products made therefrom, e.g. blocks, flakes, snacks; Liquid or semi-liquid products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/288—Taraxacum (dandelion)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
    • A61K36/346—Platycodon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/42—Cucurbitaceae (Cucumber family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488—Pueraria (kudzu)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
    • A61K36/605—Morus (mulberry)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/734—Crataegus (hawthorn)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A61K36/815—Lycium (desert-thorn)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/894—Dioscoreaceae (Yam family)
    • A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8969—Polygonatum (Solomon's seal)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention belongs to field of health care food, and in particular to one kind having multifunctional foods compositions such as auxiliary hyperglycemic, increase insulin sensitivity, liver protecting, improvement intestinal flora and its preparation method and application.The food compositions include the component of following parts by weight：20 40 parts of Chinese yam, 10 30 parts of radix polygonati officinalis, 2 20 parts of matrimony vine, 2 20 parts of PORIA ALBA, 15 parts of balsam pear, 15 parts of sealwort, 15 parts of hawthorn, 15 parts of Semen sesami nigrum, 14 parts of mulberry leaf, 14 parts of dandelion, 12 parts of pueraria lobata, 12 parts of campanulaceae.Have the function of auxiliary hyperglycemic simultaneously, increases insulin sensitivity, liver protecting, improves intestinal flora.Reliable scientific basis is provided for Clinical practice.

Description

A kind of multifunctional food composition of auxiliary hyperglycemic and preparation method thereof
Technical field
The invention belongs to field of health care food, and in particular to one kind having auxiliary hyperglycemic, increases insulin sensitivity, liver Multifunctional foods compositions such as dirty protection, improvement intestinal flora and its preparation method and application.
Background technology
Diabetes are a kind of chronic metabolic derangements diseases caused by Different types of etiopathogenises characterized by hyperglycemia.The cause of disease relates to And the various aspects such as heredity, environment, immune inflammation, the various causes of disease cause defect of insulin secretion or insulin resistance and sugar tolerance by Damage.Diabetes are broadly divided into two types by modern medicine, and one is the type 1 diabetes that insulin relies on, and another kind is non-pancreas The diabetes B that island element relies on.Diabetes not only result in the metabolic disorder of three major nutrient sugar, fat, protein, can also Cause a series of secondary diseases such as hypertension, diabetes, diabetic nephropathy, heart infarction, seriously threatens the health of the mankind. In global range, the trend risen year by year is also presented in the incidence of diabetes, now has become after cardiovascular and cerebrovascular and tumour Another big healthy " killer ", especially mid-aged population, the even more group of people at high risk of diabetes.
Diabetes are necessarily accompanied with the unbalance of blood glucose, the unbalance metabolism for being primarily due to glucose of body blood glucose It is abnormal.Pancreatic secretion insulin enters blood, and the access of insulin signaling is caused to conduct, and body intake is accelerated to utilize glucose, Glucose is occurred glycolysis or thorough redox and is supplied the energy requirement of body or be synthesized sugar by after cellular uptake It is former spare.Once any link of the above process is abnormal, it can lead to the generation of the unbalance and insulin resistance of blood glucose, and Cause diabetes.Glucose enters after cell first happens is that the phosphorylation reaction of hexokinase (HK) catalysis.Mammality is dynamic 4 kinds of hexokinase isozymes are had now been found that in object, are wherein IV types existing for liver cell, referred to as glucokinase (GK).Portugal Glucokinase is induced enzyme, which has high specificity to glucose, therefore the reaction for being catalyzed glucose is irreversible reaction. For glucose other than being aoxidized, extra meeting is synthesized glycogen, glycogen synthase kinase 3（GSK3β）Inactivation or expression under Drop can be such that the phosphorylation of glycogen synthetase declines, and then the activity of glycogen synthetase is made to increase, and glycogen is synthesized increase.
In recent years, people for diabetes occurrence and development the reason of understanding in addition to heredity, environment, immune inflammation etc. Outside, gradually it has been concerned about the exception of intestinal flora.Intestinal flora is born from us initially enters our body, adjoint throughout one's life, And our birth and old age, sickness and death are influenced, therefore intestinal flora is considered as " acquired second genome of human body ".Therefore one Some reasons of denier cause body intestinal bacilli illness, intestinal flora secretion harmful substance to enter blood, can cause Various Tissues or be Disease, including senile dementia, diabetes etc. occur for system.And some specific foods are often eaten, it can increase to a certain extent Beneficial bacterium inhibits harmful bacteria, so as to improve the intestinal flora of imbalance.
Currently, had the chemicals of the treatment diabetes much listed, such as：Insulin, melbine class, sulphonyl Ureas, alpha-glucosidase restrainer etc..Although its therapeutic effect is apparent, hypoglycemia, allergy, ametropia etc. Toxic side effect has to be noted that and take precautions against in use.Find the drug that can reduce side effect or health products Through as the present focus of attention and research hotspot.
Diabetes are under the jurisdiction of " quenching one's thirst " scope of motherland's traditional Chinese medicine, and the interpretation of the cause, onset and process of an illness is mostly weak internal organs, addiction delicious food, work and rest mistake Degree, qi depression to blood stasis etc..Motherland traditional Chinese medicine and pharmacy is of extensive knowledge and profound scholarship, and many Chinese medicines are both our usually edible food materials, and can be controlled The medicinal material of disease, referred to as integration of drinking and medicinal herbs are treated, and Chinese traditional treatment chronic disease is particular about based on dietotherapy more, Chinese traditional treatment disease tool Have action temperature and, effect is steady, and toxic side effect is small, reconciles the vital organs of the human body, reaches whole reconciliation, dialectical treatmert, giving consideration to both the incidental and fundamental Purpose, it is cheap the advantages that.
Invention content
The object of the present invention is to provide a kind of a kind of the more of auxiliary hyperglycemic being made of the Chinese medicine of integration of drinking and medicinal herbs Functional food composition and preparation method thereof.With auxiliary hyperglycemic, increase insulin sensitivity, liver protecting, improvement enteron aisle The function of flora.
To achieve the goals above, the present invention adopts the following technical scheme that：A kind of multifunctional food group of auxiliary hyperglycemic Object is closed, which includes the component of following parts by weight：20-40 parts of Chinese yam, 10-30 parts of radix polygonati officinalis, 2-20 parts of matrimony vine, in vain 2-20 parts of Poria cocos, 1-5 parts of balsam pear, 1-5 parts of sealwort, 1-5 parts of hawthorn, 1-5 parts of Semen sesami nigrum, 1-4 parts of mulberry leaf, 1-4 parts of dandelion, Pueraria lobota 1-2 parts of root, 1-2 parts of campanulaceae.
Preferably, the food compositions include the component of following parts by weight：20 parts of Chinese yam, 30 parts of radix polygonati officinalis, matrimony vine 2 Part, 2 parts of PORIA ALBA, 5 parts of balsam pear, 5 parts of sealwort, 5 parts of hawthorn, 5 parts of Semen sesami nigrum, 4 parts of mulberry leaf, 4 parts of dandelion, 2 parts of pueraria lobata, campanulaceae 2 parts.
Preferably, the food compositions include the component of following parts by weight：25 parts of Chinese yam, 25 parts of radix polygonati officinalis, matrimony vine 8 Part, 8 parts of PORIA ALBA, 3 parts of balsam pear, 3 parts of sealwort, 3 parts of hawthorn, 3 parts of Semen sesami nigrum, 3 parts of mulberry leaf, 3 parts of dandelion, 1 part of pueraria lobata, campanulaceae 1 part.
Preferably, the food compositions include the component of following parts by weight：30 parts of Chinese yam, 20 parts of radix polygonati officinalis, matrimony vine 10 Part, 10 parts of PORIA ALBA, 3 parts of balsam pear, 3 parts of sealwort, 3 parts of hawthorn, 3 parts of Semen sesami nigrum, 1 part of mulberry leaf, Pu is 1 part public, 1 part of pueraria lobata, campanulaceae 1 Part.
Preferably, the food compositions include the component of following parts by weight：40 parts of Chinese yam, 10 parts of radix polygonati officinalis, matrimony vine 20 Part, 20 parts of PORIA ALBA, 1 part of balsam pear, 1 part of sealwort, 1 part of hawthorn, 1 part of Semen sesami nigrum, 2 parts of mulberry leaf, 2 parts of dandelion, 1 part of pueraria lobata, tangerine 1 part of stalk.
In the multifunctional food composition of the auxiliary hyperglycemic, Chinese yam, radix polygonati officinalis, matrimony vine and PORIA ALBA are main components.
Further, the present invention provides the preparations that auxiliary material preparation can be added in the health-care food composition, it is preferred that this The health-care food composition of invention can be prepared into any suitable oral preparation, including tablet as needed, and capsule dissipates Agent, granule, electuary, effervescent agent or bagged instant preparation etc., the auxiliary material being added are pharmaceutically acceptable auxiliary material.
The present invention provides a kind of preparation method of the multifunctional food composition of auxiliary hyperglycemic, which includes Following steps：Corresponding medicinal material is accurately weighed, medicinal material used is cleaned with clear water, 100 DEG C, 1 hour are cooked, 65 DEG C Constant temperature oven is dried, and pulverizer beats powder, crosses 120 mesh sieve, powder is mixed evenly to prepare to required dosage form.
A kind of multifunctional food composition of auxiliary hyperglycemic is as preparing auxiliary hyperglycemic, increase insulin sensitivity Purposes in health products.
A kind of multifunctional food composition of auxiliary hyperglycemic can restore as the purposes prepared in liver protecting health products The structure of impaired liver lobuli hepatis.
A kind of multifunctional food composition of auxiliary hyperglycemic improves in intestinal bacilli illness health products as preparing to have Purposes, increase some beneficial to bacterial content, reduce some harmful bacterial contents.
Present invention can apply in different food products.
Activity is as follows the effect of each raw material in the present invention.
Chinese yam：【Nature and flavor】Sweet and neutral.【Channel tropism】Returns spleen, lung, kidney channel.【Major function】Tonifying spleen nourishing the stomach, engender liquid and benefic lung, kidney tonifying Puckery essence.For spleen eating less, endless diarrhea, the deficiency syndrome of the lung is breathed with cough, emission due to the kidney deficiency, and under band, frequent micturition, abnormal heat is quenched one's thirst.Tonifying spleen and stomach, kidney-nourishing Through；Tonifying spleen, The spleen has the function to transport and transform nutrients, therefore unfavorable for transporting, and body fluid imbalance has benefiting action；Tonifying liver, Chinese yam is sweet and slightly sour, can tonifying liver The blood of the moon；Enter kidney, it is slightly sour and restrain, some diseases caused by kidney deficiency can be treated；Enter lung, the benefits of convergence, lung can be helped to arrange absurd creature, Connect invigorating the spleen spreading body fluid；
Radix polygonati officinalis：【Nature and flavor】It is sweet, it is slightly cold.【Channel tropism】It attaches to the lung and stomach meridians.【Major function】Nourishing yin to moisten dryness promotes the production of body fluid to quench thirst.For lung stomach-Yin Wound, cough caused by dryness-heat, dry throat and mouth, Heat Diabetes；
Matrimony vine：【Nature and flavor】Sweet and neutral.【Channel tropism】Return liver and kidney channel.【Major function】It is nourishing liver and kidney, benefiting shrewd head.For consumptive disease essence Thanks to, soreness of waist and knee joint, dizziness and tinnitus, Heat Diabetes, blood deficiency chlorosis, blurred vision, tonifying kidney and benefiting sperm nourish the liver to improve visual acuity；Tonifying spleen, enhancing fortune The power of change can enrich blood well；Enter the heart, heart governing blood and vessels can be used for treating the not solid mass formed by blood stasis of blood vessels；
PORIA ALBA：【Nature and flavor】It is sweet, light, it puts down.【Channel tropism】The thoughts of returning home, lung, spleen, kidney channel.【Major function】Clearing damp and promoting diuresis, invigorating the spleen calming heart. For oedema oliguria, phlegm retention anti-dazzle nervous, spleen eating less, loose stool diarrhea, confused and worried, insomnia of palpitating with fear, strengthening the spleen and reducing phlegm, antitoxic heart-soothing and sedative； Tonifying spleen, transporting nutriment enter blood, and deficiency of enriching blood is aid digestion；Transformation of body flaud eliminates the heresy of phlegm wet, mild-natured fever and chills.
Sealwort：【Nature and flavor】Sweet and neutral.【Channel tropism】Returns spleen, lung, kidney channel.【Major function】Boosting qi and nourishing yin, invigorating the spleen, moistening lung, benefit Kidney.For weakness of the spleen and the stomach, fatigue and asthenia, dry deficiency of food, deficiency syndrome of the lung cough caused by dryness, asthenia of essence and blood, Heat Diabetes.
Hawthorn：【Nature and flavor】Sour, sweet, tepor.【Channel tropism】Returns spleen, stomach, Liver Channel.【Major function】Food digesting stomach fortifying, scattered stasis. For meat stagnation, gastral cavilty turgor, diarrhea dysentery abdominal pain, rush down dysentery is not well.
Semen sesami nigrum：【Nature and flavor】Sweet and neutral.【Channel tropism】Return liver, kidney, large intestine channel.【Major function】Filling liver kidney, benefiting essence-blood, ease constipation It is dry；
Mulberry leaf：【Nature and flavor】It is sweet, bitter, it trembles with fear.【Channel tropism】Return lung, Liver Channel.【Major function】Dispelling wind and heat from the body, clearing away the lungheat and moisturizing are clear liver and improved vision；
Dandelion：【Nature and flavor】It is bitter, sweet, it trembles with fear.【Channel tropism】Return liver, stomach.【Major function】Clearing heat and detoxicating, dispersing swelling and dissipating binds, diuresis is led to Leaching；
Pueraria lobata：【Nature and flavor】It is sweet, pungent, it is cool.【Channel tropism】Returns spleen, stomach.【Major function】Expelling pathogenic factors from muscles and skin is brought down a fever, and is promoted the production of body fluid, and promoting eruption, rising Yang stops It rushes down.For thirsty, quench one's thirst, hot dysentery, diarrhea；
Campanulaceae：【Nature and flavor】It is bitter, pungent, it puts down.【Channel tropism】It is distributed in lung channel.【Major function】Facilitaing lung, relieving sore-throat, eliminating the phlegm, apocenosis.
Compared with prior art, the innovation of the invention consists in that：
Chinese yam, radix polygonati officinalis, matrimony vine, PORIA ALBA, balsam pear, sealwort, hawthorn, Semen sesami nigrum, mulberry leaf, dandelion, pueraria lobata, 12 taste Chinese medicine of campanulaceae Material is combined, and the synergistic effect in terms of reducing blood glucose, alleviating diabetic complication achievees the effect that auxiliary hyperglycemic is same When have both increase insulin sensitivity, protection liver, improve intestinal flora health food have not been reported.12 tastes of the present invention Chinese medicine is selected from《Notice of the Ministry of Public Health about further specification healthy food material management》Defend method prison [2002] No. 51 texts of hair The medicinal material of integration of drinking and medicinal herbs in part, it is natural harmless, it is suitble to take for a long time.Whole sweet in flavor and neutral in nature not will produce cold and cool stimulation, channel tropism The upper vital organs of the human body can be taken into account.
In addition to also utilizing the online databases such as TCMSP, DAVID with reference to data, the present invention such as pharmacopeia, ancient books, report documents Bioinformatic analysis means to composition principal component Chinese yam, radix polygonati officinalis, matrimony vine, the ingredient of 4 taste Chinese medicine of PORIA ALBA, target spot carry out Screening and enrichment analysis are to support the reasonability of prescription of the present invention and comprehensive.
The present invention explains the auxiliary hyperglycemic that it has, some machines for the function of increasing insulin sensitivity from multi-angle System, including the effect for adjusting the expression of insulin signal transduction and the related gene of glucose metabolism and the enteron aisle to having lacked of proper care The improvement result of flora.
Description of the drawings
Fig. 1 is each group fasting blood sugar of 8 zoopery 1 of the embodiment of the present invention.
Fig. 2 is each group of 8 zoopery 1 of the embodiment of the present invention to 0.5 hour blood glucose value after glucose.
Fig. 3 is each group of 8 zoopery 1 of the embodiment of the present invention to 2 hours blood glucose values after glucose.
Fig. 4 is each group of 8 zoopery 1 of the embodiment of the present invention to 0 after glucose, below 0.5,2 hour blood glucose curve Product AUC.
In Fig. 1-4：Control indicates that blank group, model indicate that model group, SS-1 indicate 1 group of model+embodiment, SS-2 indicates that 2 groups of model+embodiment, SS-3 indicate that 3 groups of model+embodiment, SS-4 indicate 4 groups of model+embodiment, SS-5 Indicate that 5 groups of model+embodiment, SS-6 indicate that 6 groups of model+embodiment, SS-7 indicate 7 groups of model+embodiment.
Fig. 5 be of the invention 4 taste main ingredient Chinese yams, radix polygonati officinalis, matrimony vine, PORIA ALBA ingredient-target spot network.Wherein diamond shape represents Ingredient, circle represent target spot, and black circle represents and the relevant target spot of diabetes.
Fig. 6 is enriched to for 4 taste main ingredient Chinese yams of the invention, radix polygonati officinalis, matrimony vine, PORIA ALBA according to ingredient, target spot and diabetes 15 advanced bubble diagram before relevant bioprocess or access ranking.
Fig. 7 is each group fasting blood sugar of 12 zoopery 2 of the embodiment of the present invention.
Fig. 8 is each group of 12 zoopery 2 of the embodiment of the present invention to 0.5 hour blood glucose value after glucose.
Fig. 9 is each group of 12 zoopery 2 of the embodiment of the present invention to 2 hours blood glucose values after glucose.
Figure 10 is each group of 12 zoopery 2 of the embodiment of the present invention to 0 after glucose, under 0.5,2 hour blood glucose curve Area AUC.
Figure 11 is the result of each group insulin resistance index in 12 zoopery 2 of the embodiment of the present invention.
Figure 12 is each group liver index result in the embodiment of the present invention 13.
Figure 13 is each group renal index result in the embodiment of the present invention 13.
Figure 14 is that each group total serum protein is horizontal in the embodiment of the present invention 13.
Figure 15 is each group serum GPT levels in the embodiment of the present invention 13.
Figure 16 is each group serum creatinine level in the embodiment of the present invention 13.
Figure 17 is each group serum uric acid level in the embodiment of the present invention 13.
Figure 18 is each group liver HE coloring pathological sections in the embodiment of the present invention 14.
Figure 19 is each group liver glucose kinases in the embodiment of the present invention 15（GK）MRNA expressions.
Figure 20 is each group liver glycogen synthase kinase in the embodiment of the present invention 15（GSK3β）MRNA expressions.
In Fig. 7-17, Figure 19-20：Control indicates that blank group, normal expressions normally give high agent group, and SS-L is indicated Model+low dose of group, SS-M indicate that model+middle agent group, SS-H indicate that model+high agent group, positive indicate positive control Group.
Figure 21 is the result figure of intestinal flora in the embodiment of the present invention 16.
Specific implementation mode
Results of statistical analysis uses SPSS processing, and P≤0.05 thinks there is significant difference.
One, the screening test of active ingredient of the present invention.
Embodiment 1：Weigh 20 parts of Chinese yam, 30 parts of radix polygonati officinalis, 2 parts of matrimony vine, 2 parts of PORIA ALBA, 5 parts of balsam pear, 5 parts of sealwort, hawthorn 5 Part, 5 parts of Semen sesami nigrum, 4 parts of mulberry leaf, 4 parts of dandelion, 2 parts of pueraria lobata, 2 parts of campanulaceae clean medicinal material used with clear water, 100 DEG C, cooked within 1 hour, the drying of 65 DEG C of constant temperature ovens, pulverizer beats powder, 120 mesh sieve of mistake, by powder mixing.
Embodiment 2：Weigh 25 parts of Chinese yam, 25 parts of radix polygonati officinalis, 8 parts of matrimony vine, 8 parts of PORIA ALBA, 3 parts of balsam pear, 3 parts of sealwort, hawthorn 3 Part, 3 parts of Semen sesami nigrum, 3 parts of mulberry leaf, 3 parts of dandelion, 1 part of pueraria lobata, 1 part of campanulaceae, the preparation method is the same as that of Example 1.
Embodiment 3：Weigh 30 parts of Chinese yam, 20 parts of radix polygonati officinalis, 10 parts of matrimony vine, 10 parts of PORIA ALBA, 3 parts of balsam pear, 3 parts of sealwort, mountain 3 parts of short, bristly hair or beard, 3 parts of Semen sesami nigrum, 1 part of mulberry leaf, Pu is 1 part public, and 1 part of pueraria lobata, 1 part of campanulaceae, the preparation method is the same as that of Example 1.
Embodiment 4：Weigh 40 parts of Chinese yam, 10 parts of radix polygonati officinalis, 20 parts of matrimony vine, 20 parts of PORIA ALBA, 1 part of balsam pear, 1 part of sealwort, mountain 1 part of short, bristly hair or beard, 1 part of Semen sesami nigrum, 2 parts of mulberry leaf, 2 parts of dandelion, 1 part of pueraria lobata, 1 part of campanulaceae.The preparation method is the same as that of Example 1.
Embodiment 5：Weigh 30 parts of Gorgon fruit, 20 parts of radix polygonati officinalis, 10 parts of matrimony vine, 10 parts of PORIA ALBA, 3 parts of balsam pear, 3 parts of sealwort, mountain 3 parts of short, bristly hair or beard, 3 parts of Semen sesami nigrum, 1 part of mulberry leaf, Pu is 1 part public, and 1 part of pueraria lobata, 1 part of campanulaceae, the preparation method is the same as that of Example 1.
Embodiment 6：Weigh 30 parts of Chinese yam, 20 parts of Radix Glycyrrhizae, 10 parts of matrimony vine, 10 parts of PORIA ALBA, 3 parts of balsam pear, 3 parts of sealwort, mountain 3 parts of short, bristly hair or beard, 3 parts of Semen sesami nigrum, 1 part of mulberry leaf, Pu is 1 part public, and 1 part of pueraria lobata, 1 part of campanulaceae, the preparation method is the same as that of Example 1.
Embodiment 7：Weigh 30 parts of Chinese yam, 20 parts of radix polygonati officinalis, 10 parts of mulberries, 10 parts of PORIA ALBA, 3 parts of balsam pear, 3 parts of sealwort, mountain 3 parts of short, bristly hair or beard, 3 parts of Semen sesami nigrum, 1 part of mulberry leaf, Pu is 1 part public, and 1 part of pueraria lobata, 1 part of campanulaceae, the preparation method is the same as that of Example 1.
8 zoopery 1 of embodiment
Sample：Health-care food composition prepared by embodiment 1-7, is dispersed to respectively in the water of 140ml volumes.
Main agents and instrument：Blood glucose meter.
Animal：SPF rank male rats, 180 ± 20g, by Liaoning, long-living Bioisystech Co., Ltd provides.
8.1 groupings and administration
（1）Healthy adult animal 90 is chosen,（SPF rank male rats, 180 ± 20g）, common that feed is maintained to adapt to raising 7 It, measure fasting blood-glucose, based on be worth, randomly select 10 and be only used as blank group.
（2）Remaining 80 subsequent fasting 24 hours（Free water）Afterwards, alloxan 150mg/kg intraperitoneal injections are given（Face With now matching, normal saline is injected in 30 minutes and is finished）Make hyperglycemia model.After 5 days, fasting 4 hours takes tail blood, measures Blood glucose value is all higher than 10mmol/L, modeling success.It is randomly divided into model group, 1 group of model+embodiment, model+embodiment 2 3 groups of group, model+embodiment, 4 groups of model+embodiment, 5 groups of model+embodiment, 6 groups of model+embodiment, model+implementation 7 groups of example, every group 10.
（3）Blank control group, model control group gavage give same volume solvent, i.e. distilled water, 1ml/100g；Remaining each group Gavage gives corresponding example composition formula, 1ml/100g, continuous 35 days.
（4）After the test, all animals fasting 4 hours take tail hematometry blood glucose and sugar tolerance.
8.2 carbohydrate tolerance test
Blank control group is not processed, and model control group gives same volume solvent, remaining each group gives corresponding dosage composition, and 20 Each group oral administration of glucose 2.5g/kg after minute takes tail blood, measurement to give each group after glucose 0.5,2 hour blood glucose value, and 0,0.5,2 Area under the curve of blood glucose AUC of calculating=（+ 0.5 hour blood glucose of 0 hour blood glucose）*0.5/2+（0.5 hour blood glucose+2 hours Blood glucose）*1.5/2.
Embodiment 9：Ingredient, the target spot of 4 taste main ingredient materials in the present composition are sieved using bioinformatics method Choosing and enrichment analysis
（1）4 taste Chinese medicines are separately input to TCMSP（http://lsp.nwu.edu.cn/tcmspsearch.php）Data In library, by all the components according to pharmacokinetic parameter, oral administration biaavailability OB >=30%, quasi-medicated property DL >=0.18 or oral Bioavilability OB >=30%, half-life period HL >=4 condition screened.
（2）The ingredient that will be screened, the target spot in correspondence database.
（3）The target spot screened is input to Uniprot（http://www.uniprot.org/）Specification target in database Point title, its corresponding Gene Name.
（4）The Gene Name of all target spots is uploaded to DAVID（https://david.ncifcrf.gov/ summary.jsp）Database carries out the access enrichment analysis of the bioprocess and KEGG of GO-BP, filters out and occur with diabetes Develop relevant target gene.
（5）The network struction that selected ingredient, target spot are carried out to ingredient-target spot using Cytoscape3.2.1 softwares, is painted The relevant bioprocess of diabetes processed or the advanced bubble diagram of access.
Embodiment 10：Prepare the composition of the basic, normal, high dosage according to the share ratio in embodiment 3, i.e. embodiment 3 It is low dosage composition, middle dosage composition each component content is 5 times of embodiment 3, and high dose composition each component content is 10 times of embodiment 3, are dispersed to respectively in the water of 140ml volumes.
Embodiment 11：The preparation of positive control
Positive control drug Tongrentang board pueraria lobata Chinese yam capsules 3.90g is weighed, is dispersed in 140ml volume clear water, it is spare.
Embodiment 12：Zoopery 2
Sample：Sample prepared by embodiment 10,11.
Main agents and instrument：Rat blood serum insulin ELISA kit, blood glucose meter, full-automatic biochemical detector.
Animal：SPF rank male rats, 180 ± 20g, by Liaoning, long-living Bioisystech Co., Ltd provides.
12.1 groupings and administration
（1）Healthy adult animal 70 is chosen,（SPF rank male rats, 180 ± 20g）, common that feed is maintained to adapt to raising 7 It, measures fasting blood-glucose, based on be worth, randomly select 20 respectively as blank group, normal high agent group（That is not modeling is given Give high dose）, every group 10.
（2）Remaining 50 subsequent fasting 24 hours（Free water）Afterwards, alloxan 150mg/kg intraperitoneal injections are given（Face With now matching, normal saline is injected in 30 minutes and is finished）Make hyperglycemia model.After 5 days, fasting 4 hours takes tail blood, measures Blood glucose value is all higher than 10mmol/L, modeling success.Be randomly divided into model control group, model+low dose of group, model+middle agent group, Model+high agent group, positive controls, every group 10.
（3）Blank control group, model control group gavage give same volume solvent, i.e. distilled water, 1ml/100g；Remaining each group Gavage gives embodiment the corresponding combination composition formula, 1ml/100g, continuous 35 days.
（4）After the test, all animals fasting 4 hours take tail hematometry blood glucose and sugar tolerance, take venous blood under abdominal cavity Liquid centrifuges serum and measures serum insulin content, wins the tissues such as liver, kidney and weighs weight, and takes liver same area one Point, it is stored in -80 DEG C of refrigerators for use, a part is stored in the liquid of paraformaldehyde for use.
12.2 sugar tolerance is tested：Experiment is the same as embodiment 8.2.
The measurement of 12.3 insulin resistance indexes
Each group animal fasting 4 hours, takes rat blood, is centrifuged out serum, and ELISA method detects the content of serum insulin.
（1）The dilution of standard items：It takes standard items 120ul that isometric standard dilutions are added as No. 5 standard items, takes 5 Isometric standard dilutions are added as No. 4 standard items in number standard items 120ul, and so on, it is diluted to 3,2, No. 1 standards Product.
（2）Blank well is not added with anti-INS antibody, the Streptavidin-HRP of sample, biotin labeling, only adds color developing agent A, B And terminate liquid, remaining each step operation are identical.
（3）Standard items 50ul, Streptavidin-HRP 50ul is added in standard sample wells（Standard items have integrated biotin Antibody）.
（4）Sample 40ul is added in sample to be tested hole, and anti-INS antibody 10ul, Streptavidin-HRP 50ul is added.
（5）After adding above, sealing plate film is covered, gently shakes mixing, 37 DEG C incubate 60 minutes.
（6）It takes sealing plate film off, discards waste liquid, dry, fill it up with cleaning solution per hole, discarded after standing 30 seconds, repeatedly 5 It is secondary, it pats dry.
（7）Color developing agent A liquid 50ul are first added in hole, add color developing agent B liquid 50ul（B liquid pays attention to being protected from light）, 37 DEG C are protected from light temperature It educates 15 minutes.
（8）Terminate liquid 50ul is added, terminates reaction, with blank well school zero, 450nm wavelength sequentially measures absorbance OD values （Measurement is completed in 10 minutes）.
（9）According to OD values, standard curve is drawn, the concentration of each insulin is calculated（Standard curve OD=standard items OD-blank OD, the practical OD of experimental group=experimental group OD-blank OD）.
（10）Calculate insulin/22.5 insulin resistance index ≈ fasting blood-glucose *.
The detection of the calculating and hepatic and renal function of 13 organ index of embodiment.
At the end of 13.1 experiments, the liver won, kidney are weighed and recorded, and calculates organ index=internal organs weight Amount（g）The weight of/the experimental animal（g）*100%.
13.2 full automatic biochemical apparatus detect liver function index in serum（Total protein, alkaline phosphatase）And renal function index （Creatinine and urea nitrogen）Content.
14 hepatic pathology section of embodiment.
14.1 wax embeddings
（1）Flowing water rinse 6 hours, 70%, 80%, 90%, 95%, absolute ethyl alcohol graded ethanol impregnate.
（2）Dimethylbenzene 1,2 impregnates.
（3）Packet wax stone.
（4）Microtome.
14.2 HE is dyed
（1）Roasting piece machine handcuff piece 2 hours.
（2）Dimethylbenzene 1,2 dewaxes, and crosses absolute ethyl alcohol, 95%, 85%, 75% graded ethanol.
（3）Tap water rinses, haematoxylin dyeing, and tap water rinses.
（4）1% hydrochloride alcohol, tap water rinse, and eosin stains, tap water rinses.
（5）75%, 85%, 95%, absolute ethyl alcohol gradient elution, dimethylbenzene 1,2 elute.
（6）Resinene mounting, dries, micro- sem observation.
The detection of gene expression dose in 15 liver of embodiment.
Main agents and instrument：Trizol reagents, DNA reverse transcription reagent box, DNA cloning kit, PCR amplification instrument etc..
11.1 extraction liver organization total serum IgEs
（1）The liver organization of -80 DEG C of freezen protectives is taken out, about 50 mg tissues is cut with scalpel, is put into and is equipped with In the test tube without RNA enzyme of Trizol.
（2）Tissue is broken into homogenate, and mixing as possible with refiner, is stored at room temperature 5 minutes.
（3）It 4 DEG C, 12000 g, centrifuges 5 minutes.Supernatant is transferred in the centrifuge tube of new no RNA enzyme, according to 5: Chloroform is added in 1 ratio, acutely shakes 30 s or so mixing, is stored at room temperature 5 minutes.
（4）It 4 DEG C, 12000 g, centrifuges 15 minutes.Supernatant liquid is transferred in the centrifuge tube of new no RNA enzyme.
（5）Isometric isopropanol is added, mildly overturns mixing up and down, is stored at room temperature 10 minutes.
（6）It 4 DEG C, 12000 g, centrifuges 10 minutes.
（7）Liquid carefully is discarded supernatant, 75 % ethyl alcohol, 1 ml of -20 DEG C of precoolings is incorporated in into precipitation（DEPC water configures）, Upper and lower mixing cleaning precipitation.
（8）It 4 DEG C, 12000g, centrifuges 5 minutes.
（9）Liquid carefully is discarded supernatant, is dried, is precipitated with DEPC water dissolutions, is stored in -80 DEG C.
11.2 mRNA reverse transcriptions
（1）The total serum IgE extracted from liver is taken into 2 ul, measured with ultramicron uv analyzer and records its concentration.
（2）According to the concentration surveyed, required RNA volumes are calculated according to the amount of 1 ug of reverse transcription.
（3）It is added in corresponding reaction tube according to the template ribonucleic acid of calculating and the amount of water, often pipe contains primer Oligo (dT), gDNA Remover, Enzyme-Mix, 2 × Reaction-Mix, RNA, DEPC water.
（4）After mixing well, it is put into PCR amplification instrument and carries out reverse transcription.
（5）Gained complementary DNA（cDNA）, spare.
11.3 real time fluorescent quantitative expands（qPCR）
The reference gene of selection is glyceraldehyde-3-phosphate dehydrogenase（GAPDH）, target gene is glucokinase, Glycogen synthesis Work synthesis is given birth in 3 β of kinase enzyme, Shanghai.
（1）Primer 12000g is centrifuged for 15 minutes, and often corresponding Rase-free Water are added in pipe, then dilute 10 times it is standby With.
（2）According to experimental measuring, often pipe is added template cDNA, upstream and downstream primer, Master Mix, add DEPC water mend to 50ul。
（3）Mentioned component is added in 96 orifice plates of eight townhouses, is put into Real Time PCR System instruments and carries out Amplification and real-time quantitative measure.
The detection of 16 intestinal flora of embodiment
Detection group is blank group, model group, model+middle agent group.
16.1 MetaVx library constructions and Illumina MiSeq sequencings
（1）The dense of DNA sample is detected using 2.0 Fluorometer of Qubit (Invitrogen, Carlsbad, CA) Degree, uses MetaVx library construction Kits, sequencing library.
（2）Using 30-50ng DNA as template, a series of PCR primer amplifications prokaryotes of gold only intelligence design are used Include 2 hypervariable regions of V3 and V4 on 16S rDNA.
（3）Using 2100 biological analysers of Agilent (Agilent Technologies, Palo Alto, CA, USA Library Quality) is detected, and is detected by Qubit2.0 Fluorometer (Invitrogen, Carlsbad, CA) Library concentration.
16.2 results are depicted as thermal map
Experimental result
1. each group fasting blood sugar and sugar tolerance of 8 zoopery 1 of the embodiment of the present invention are as a result, as shown in table 1 and Fig. 1-4.
Embodiment 5-7 can be obtained from result to the side's of tearing open example of the isodisperse of the embodiment of the present invention 3 respectively：Model group blood Sugar increases obviously, and the fasting blood-glucose of each group of the present invention gives 0.5,2 hour blood glucose and Area under the curve of blood glucose and mould after glucose Type group compares that decline is apparent, illustrates that the composition of the present invention has a hypoglycemic effect, and the side's of tearing open each group fasting blood-glucose, to after glucose 0.5,2 hours blood glucose and Area under the curve of blood glucose be not variant compared with model group, illustrate the present invention reasonable recipe and Effectively.Comprehensive each group, 3 groups of embodiment is optimal set.
The ingredient of 4 taste Chinese medicine of 2.TCMSP databases pair, target spot analysis result, as shown in table 2 and Fig. 5-6.
The 4 taste primary raw material Chinese medicines of the present invention filter out 103 lateral reactivity ingredients, 197 target spots, wherein being enriched to altogether Total 81 with the relevant target spot of diabetes, account for 1/3 or more of screening target spot.Before ranking 15 access all with sugar, fat generation Journey of apologizing for having done sth. wrong is closely bound up, from the angle of big data bioinformatic analysis, absolutely proves the reasonability of prescription of the present invention and has Auxiliary hyperglycemic, improve insulin sensitivity functional potentiality.
3. each group fasting blood-glucose of 12 zoopery 2 of embodiment and sugar tolerance as a result, as shown in table 3 and Fig. 7-10.
It can be obtained from result：For model group compared with blank group, fasting blood sugar gives 0.5 hour blood glucose after glucose Value, to 2 hours blood glucose values after glucose, to 0 after glucose, 0.5,2 hour blood glucose value area under the curve AUC value increase it is bright Aobvious, difference has statistical significance；
Model+low dose of group, model+middle agent group, model+high agent group and the positive controls of the present invention are empty compared with model group Abdomen blood glucose value, to 0.5 hour blood glucose value after glucose, to 2 hours blood glucose values after glucose, to 0,0.5,2 hour after glucose Blood glucose value area under the curve AUC value declines obviously, and difference has statistical significance.Blood sugar decreasing effect of the present invention exists with dosage Relationship, middle dose of low dose of ＞ high agent ＞.
Blank+high agent group, i.e., the high dose group that intact animal is given, compared with blank group, fasting blood sugar gives grape 0.5 hour blood glucose value after sugar, to 2 hours blood glucose values after glucose, to 0,0.5,2 hour blood glucose value area under the curve after glucose There is no significant differences by AUC.
4. the result of calculation of each group insulin resistance index, as shown in table 4 and Figure 11.
It can be obtained from result：Model group is compared with blank group, and insulin resistance index value increases obviously, and difference has Statistical significance；
Model+low dose of group, model+middle agent group, model+high agent group and the positive controls of the present invention are compared with model group, pancreas Insulin resistance exponential quantity declines obviously, and difference has statistical significance.The present invention increases insulin sensitivity effect and dosage There are relationship, middle dose of low dose of ＞ high agent ＞.
Blank+high agent group, i.e., the high dose group that intact animal is given, insulin resistance index value is simultaneously compared with blank group There is no significant difference.
5. 13 organ index of the embodiment of the present invention is as a result, as shown in table 5 and Figure 12-13.
It can be obtained from result：Compared with blank group, organ index is increased model group, but does not have statistics Difference.
Model+low dose of group, model+middle agent group, model+high agent group and the positive controls and model group ratio of the present invention Compared with organ index value is declined, but does not have significant difference.
Blank+high agent group, i.e., the high dose group that intact animal is given, there is no unite for internal organs exponential quantity compared with blank group Meter learns difference.
6 embodiment of the present invention, 13 liver, renal function index are as a result, as shown in table 6 and Figure 14-17.
It can be obtained from result：Total serum protein, creatinine level and the blank group of model group are increased than, but not With significant difference；Model+low dose of group, model+middle agent group, model+high agent group and the positive are to organizing the blood compared with model group Clear total protein, creatinine level decline, and do not have significant difference.
Serum glutamic pyruvic transminase, uric acid level and the blank group of model group are more apparent than increasing, and have significant difference；Model + low dose of group, model+middle agent group, model+high agent group and the positive are to organizing serum glutamic pyruvic transminase, uric acid water compared with model group It is flat to decline obviously, there is statistical significance.
Blank+high agent group, i.e., the high dose group that intact animal is given, compared with blank group, total serum protein, creatinine, paddy Pyruvic transaminase, uric acid level do not have difference.
7 each group hepatic pathology HE coloration results, as shown in figure 18.
It can be obtained from result：The lobuli hepatis of the liver organization of model group changes with blank group comparative structure, liver rope Structure disturbance, cell arrangement is irregular, and central vein region becomes smaller；Model+middle agent group can restore disorderly lobuli hepatis substantially Structure approaches blank group, and better than the change of positive controls；Model+low dose, the lobuli hepatis structure damage of high agent group are also equal It makes moderate progress；Blank+high agent group（The high dose group that i.e. intact animal is given）, compared with blank group, there is no pathology occurs to change Become.
The mRNA expressions of 8 each group hepatic genes are as a result, such as table 7, shown in Figure 19-20.
It can be obtained from result：Model group is compared with blank group, liver glucose kinases（GK）MRNA level in-site decline it is bright Aobvious, difference has statistical significance；Model+low dose of group, model+middle agent group, model+high agent group, positive controls and model group Compare, liver glucose kinases（GK）MRNA level in-site increase apparent, difference has statistical significance.Blank+high agent group, i.e., The high dose group that intact animal is given, compared with blank group, liver glucose kinases（GK）MRNA level in-site there is no statistics Difference.
Model group is compared with blank group, 3 β of liver glycogen synthase kinase（GSK3β）MRNA level in-site increase apparent, difference With statistical significance；Model+low dose of group, model+middle agent group, model+high agent group, positive controls are compared with model group, liver Dirty glycogen synthase kinase 3 β（GSK3β）MRNA level in-site decline apparent, difference has statistical significance.
Blank+high agent group, i.e., the high dose group that intact animal is given, compared with blank group, liver glycogen synthase kinase 3β（GSK3β）MRNA level in-site there is no significant differences.
9 intestinal floras are as a result, as shown in table 8 and Figure 21.
It can be obtained from result：The result of intestinal flora is shown：Be conducive to some bacterium such as Mao sieve bacterium of human body in model group Middle to decline obviously, its content increased in the present invention；Some are unfavorable for bacterium such as Pasteurella, Fusobacterium, the deformed rod of human body Bacterium, streptococcus are increased in model group, and the present invention can reduce these raised floras, illustrate that the present invention but has improvement The function of disorderly intestinal flora.
It summarizes：Animal experiments show that the present invention is compared with model group, glucose is given in fasting blood sugar, sugar tolerance experiment 0.5,2 hour blood glucose value and 0,0.5,2 hour Area under the curve of blood glucose decline obviously afterwards, illustrate present invention auxiliary drop blood The function of sugar；Downward trend is more presented with model group in insulin resistance index of the present invention, there is significant difference, illustrates this Invention has the function of insulin sensitivity enhancing；Blank+high agent group, i.e. intact animal give the high dose group of health food, 0, 0.5,2 hours blood glucose values and 0,0.5,2 hour Area under the curve of blood glucose, insulin resistance index do not occur significantly to become Change, illustrates that taking this health food for normal group not will produce blood glucose fluctuation or insulin resistance, can also trust and eat this guarantor Health food.
The change of liver and renal index, the detection of serum kidney function indicator, hepatic pathology section HE coloration results are said The bright present invention has certain liver renal protection, also has certain restitution to the pathology damage of diabetes liver.
The detection of the mRNA level in-site of gene proves that the present invention can adjust glucose sugar kinases and glycogen synthase kinase in liver The expression of 3 gene of enzyme, is conducive to the decline of blood glucose, illustrates that this health food can adjust insulin signal transduction and glucose generation The expression of relevant gene during thanking.
The result of intestinal flora illustrates that the present invention can increase the content of probiotics, reduces the flora unfavorable to human body, from And improve disorderly intestinal flora, be conducive to body health.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, any made by repair Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.

Claims (9)

1. a kind of multifunctional food composition of auxiliary hyperglycemic, which is characterized in that the food compositions include following parts by weight Several components：20-40 parts of Chinese yam, 10-30 parts of radix polygonati officinalis, 2-20 parts of matrimony vine, 2-20 parts of PORIA ALBA, 1-5 parts of balsam pear, 1-5 parts of sealwort, 1-5 parts of hawthorn, 1-5 parts of Semen sesami nigrum, 1-4 parts of mulberry leaf, 1-4 parts of dandelion, 1-2 parts of pueraria lobata, 1-2 parts of campanulaceae.
2. a kind of multifunctional food composition of auxiliary hyperglycemic according to claim 1, which is characterized in that the food group Close the component that object includes following parts by weight：20 parts of Chinese yam, 30 parts of radix polygonati officinalis, 2 parts of matrimony vine, 2 parts of PORIA ALBA, 5 parts of balsam pear, sealwort 5 Part, 5 parts of hawthorn, 5 parts of Semen sesami nigrum, 4 parts of mulberry leaf, 4 parts of dandelion, 2 parts of pueraria lobata, 2 parts of campanulaceae.
3. a kind of multifunctional food composition of auxiliary hyperglycemic according to claim 1, which is characterized in that the food group Close the component that object includes following parts by weight：25 parts of Chinese yam, 25 parts of radix polygonati officinalis, 8 parts of matrimony vine, 8 parts of PORIA ALBA, 3 parts of balsam pear, sealwort 3 Part, 3 parts of hawthorn, 3 parts of Semen sesami nigrum, 3 parts of mulberry leaf, 3 parts of dandelion, 1 part of pueraria lobata, 1 part of campanulaceae.
4. a kind of multifunctional food composition of auxiliary hyperglycemic according to claim 1, which is characterized in that the food group Close the component that object includes following parts by weight：30 parts of Chinese yam, 20 parts of radix polygonati officinalis, 10 parts of matrimony vine, 10 parts of PORIA ALBA, 3 parts of balsam pear, sealwort 3 parts, 3 parts of hawthorn, 3 parts of Semen sesami nigrum, 1 part of mulberry leaf, Pu is 1 part public, 1 part of pueraria lobata, 1 part of campanulaceae.
5. a kind of multifunctional food composition of auxiliary hyperglycemic according to claim 1, which is characterized in that the food group Close the component that object includes following parts by weight：40 parts of Chinese yam, 10 parts of radix polygonati officinalis, 20 parts of matrimony vine, 20 parts of PORIA ALBA, 1 part of balsam pear, sealwort 1 part, 1 part of hawthorn, 1 part of Semen sesami nigrum, 2 parts of mulberry leaf, 2 parts of dandelion, 1 part of pueraria lobata, 1 part of campanulaceae.
6. a kind of multifunctional food composition of auxiliary hyperglycemic according to claim 1, which is characterized in that the food group Conjunction object can be added auxiliary material and various preparations, preferably oral preparation, including tablet, capsule, powder, granule, electuary, effervesce is made Agent or bagged instant preparation etc., auxiliary material used are generally pharmaceutically acceptable auxiliary material.
7. a kind of preparation method of the multifunctional food composition of auxiliary hyperglycemic as described in claim 1, the preparation method Include the following steps：Corresponding medicinal material is accurately weighed, medicinal material used is cleaned with clear water, 100 DEG C, 1 hour are cooked, 65 DEG C of constant temperature oven drying, pulverizer beat powder, cross 120 mesh sieve, powder is mixed evenly to prepare to required dosage form.
It prepares auxiliary hyperglycemic 8. a kind of multifunctional food composition of auxiliary hyperglycemic according to claim 1 is used as, increase Purposes in insulin sensitivity, liver protecting health products, in the health products of improvement intestinal flora.
9. being also applied in different food according to a kind of multifunctional food composition of auxiliary hyperglycemic of claim 1.
CN201810448001.0A 2018-05-11 2018-05-11 Multifunctional food composition for assisting in reducing blood sugar and preparation method thereof Active CN108514093B (en)

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