CN108504563A - 水平式胞浆内单精子注射用精子优选装置及精子优选方法 - Google Patents

水平式胞浆内单精子注射用精子优选装置及精子优选方法 Download PDF

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CN108504563A
CN108504563A CN201810648348.XA CN201810648348A CN108504563A CN 108504563 A CN108504563 A CN 108504563A CN 201810648348 A CN201810648348 A CN 201810648348A CN 108504563 A CN108504563 A CN 108504563A
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李楠
林忠
李忻琳
韦立红
王麟
李红辉
仇雪嫦
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Liuzhou Maternity and Child Healthcare Hospital
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Abstract

本发明涉及一种水平式胞浆内单精子注射用精子优选装置及精子优选方法，该精子优选装置包括精子优选槽体，所述精子优选槽体内通过分隔板分隔形成精子储存池和优质精子池，所述分隔板上开设有精子游动通道，该精子游动通道连通精子储存池和优质精子池，精子储存池和优质精子池下方分别设置有恒温加热板Ⅰ和恒温加热板Ⅱ；该精子优选方法利用精子储存池和优质精子池之间的温度度差，使活动力好的精子从温度低的精子储存池通过精子游动通道游向温度高的优质精子池中。本发明能够对梯度离心方法获得的精子进行进一步优选，选择出具有较好活力的精子，用选择后的精子进行受精，提高ICSI的受精率和ICSI后胚胎的发育能力，最终提高临床妊娠率。

Description

水平式胞浆内单精子注射用精子优选装置及精子优选方法
技术领域
本发明涉及一种精子优选装置及方法，特别涉及一种水平式胞浆内单精子注射用精子优选装置及精子优选方法。
背景技术
卵胞浆内单精子显微注射技术（ICSI），也就是第二代“试管婴儿”，该技术是借助显微操作系统将单一精子注射入卵子内使其受精。
由于进行ICSI主要针对的是精子质量很差或者数量很少的患者，这些患者的精子往往存在多种缺陷，比如顶底反应差，数量少，活力差，密度低等问题，因此需要进行ICSI操作。ICSI操作时，在显微镜下用ICSI针吸取活动的精子，然后将精子注射到卵母细胞的胞质中，完成受精的过程。ICSI操作时无法对精子质量进行筛选，仅仅是在显微镜下可以看到的精子，用ICSI针抓取后，注射到胞质中。因此出现ICSI胚胎发育能力差，妊娠率较低的结果，还会出现ICSI后仍然无法受精，无法发育的情况。这样对患者的身体和心理造成损失，包括金钱、时间等多方面的损失，无法受精发育后还需要进行下一次治疗，延误病情，无法在最适合受孕的时间获得受孕。
目前无专用的卵胞浆内单精子注射（ICSI）精子分选装置，精子处理一般是采用梯度离心或者直接离心的方法。梯度离心或者直接离心的方法，是将精子与精清进行了分离，去除杂质，并将有活力的精子和无活力的精子进行分离，但是通过上述方法分离得到的有活力的精子中，也会存在质量较差的精子，这是梯度离心或者直接离心存在的不足之处，故需要对精子进行进一步的优选。
发明内容
本发明要解决的技术问题是：提供一种水平式胞浆内单精子注射用精子优选装置及精子优选方法，采用该装置及方法能够对梯度离心方法获得的精子进行进一步优选，选择出具有较好活力的精子，用选择后的精子进行受精，提高ICSI的受精率和ICSI后胚胎的发育能力，最终提高临床妊娠率，解决了上述现有技术存在的问题。
解决上述技术问题的技术方案是：一种水平式胞浆内单精子注射用精子优选装置，包括精子优选槽体，所述精子优选槽体内通过分隔板分隔形成精子储存池和优质精子池，所述分隔板上开设有精子游动通道，该精子游动通道连通精子储存池和优质精子池，精子储存池下方设置有用于加热精子储存池的恒温加热板Ⅰ，优质精子池下方设置有用于加热优质精子池的恒温加热板Ⅱ。
所述精子游动通道的长度为8-30mm，深度为6-12mm，宽度为6-12mm。
所述精子储存池和优质精子池的深度为6-12mm。
所述恒温加热板Ⅰ和恒温加热板Ⅱ下端还设置有支撑腿。
本发明的另一技术方案是：一种水平式胞浆内单精子注射用精子优选方法，采用上述的精子优选装置，包括以下步骤：
⑴在精子储存池和优质精子池内加入洗净液，然后开启恒温加热板Ⅰ和恒温加热板Ⅱ；
⑵控制精子储存池内的液体温度为34.5-35.5℃，优质精子池内的液体温度为37.5-38.5℃，将经过梯度离心或者直接离心处理后的精子放入精子储存池内，活力好的精子通过精子游动通道游向优质精子池，从精子放入精子储存池内开始计时，10-20min后，取优质精子池内的精子即为活力好的精子。
精子具有趋热性，当温度出现差异时，精子会游向温度较高的区域。本发明控制精子储存池的温度低、优质精子池的温度高，利用精子储存池和优质精子池之间的温度度差，使活动力好的精子从温度低的精子储存池通过精子游动通道游向温度高的优质精子池中，而活力差的精子始终留在精子储存池中，起到分离活力好和活力差精子的目的。
采用本发明对梯度离心方法获得的精子进行进一步优选，选择出具有较好活力的精子，用选择后的精子进行受精，提高ICSI的受精率和ICSI后胚胎的发育能力，最终提高临床妊娠率，维护患者权益，提高患者满意度。
下面，结合附图和实施例对本发明之水平式胞浆内单精子注射用精子优选装置及精子优选方法的技术特征作进一步的说明。
附图说明
图1：本发明之水平式胞浆内单精子注射用精子优选装置结构示意图。
图2：图1的A-A剖视图。
图中：1-精子优选槽体，11-精子储存池，12-优质精子池，2-分隔板，21-精子游动通道，3-恒温加热板Ⅰ，4-恒温加热板Ⅱ，5-支撑腿。
具体实施方式
实施例1：一种水平式胞浆内单精子注射用精子优选装置，如图1-图2所示，包括精子优选槽体1，所述精子优选槽体1内通过分隔板2分隔形成精子储存池11和优质精子池12，所述分隔板2上开设有精子游动通道21，该精子游动通道连通精子储存池和优质精子池，精子储存池11下方设置有用于加热精子储存池的恒温加热板Ⅰ3，优质精子池12下方设置有用于加热优质精子池的恒温加热板Ⅱ4。恒温加热板Ⅰ和恒温加热板Ⅱ分别固定在精子优选槽体1下端面。
本实施例中，所述精子游动通道的长度为8-30mm，优选20mm，深度为6-12mm，优选10mm，宽度为6-12mm，优选10mm。所述精子储存池和优质精子池的深度为6-12mm，优选10mm，底面的厚度为5-8mm，精子储存池和优质精子池的底面不宜大厚，以免不利于加热。作为一种变换，精子游动通道、精子储存池和优质精子池的具体尺寸还可以根据实际情况调整变化。
本实施例中，所述恒温加热板Ⅰ和恒温加热板Ⅱ下端还设置有支撑腿5。作为一种变换，也可以不设置支撑腿。
本实施例中恒温加热板的具体结构与现有技术相同，此处不再赘述。
实施例2：一种水平式胞浆内单精子注射用精子优选方法，采用实施例1所述的精子优选装置，包括以下步骤：
⑴在精子储存池和优质精子池内加入洗精液，然后开启恒温加热板Ⅰ和恒温加热板Ⅱ；
⑵控制精子储存池内的液体温度为35℃，优质精子池内的液体温度为38℃，将经过梯度离心或者直接离心处理后的精子放入精子储存池内，活力好的精子通过精子游动通道游向优质精子池，从精子放入精子储存池内开始计时，10-20min后，取优质精子池内的精子即为活力好的精子。
注：精子活动力分为4级：A级表示精子活动极好，呈快速直线向前运动；B级表示精子活动很好，直线向前运动；C级表示精子活动力一般，只向前曲线运动；D级表示精子活动能力差，只在原地蠕动。本发明精子的活力采用现有精液分析系统检测。
从表中可以看出，采用本发明分离后精子活力显著高于分离前精子活力。

Claims (5)

1.一种水平式胞浆内单精子注射用精子优选装置，其特征在于：包括精子优选槽体，所述精子优选槽体内通过分隔板分隔形成精子储存池和优质精子池，所述分隔板上开设有精子游动通道，该精子游动通道连通精子储存池和优质精子池，精子储存池下方设置有用于加热精子储存池的恒温加热板Ⅰ，优质精子池下方设置有用于加热优质精子池的恒温加热板Ⅱ。
2.根据权利要求1所述的水平式胞浆内单精子注射用精子优选装置，其特征在于：所述精子游动通道的长度为8-30mm，深度为6-12mm，宽度为6-12mm。
3.根据权利要求1或2所述的水平式胞浆内单精子注射用精子优选装置，其特征在于：所述精子储存池和优质精子池的深度为6-12mm。
4.根据权利要求1或2所述的水平式胞浆内单精子注射用精子优选装置，其特征在于：所述恒温加热板Ⅰ和恒温加热板Ⅱ下端还设置有支撑腿。
5.一种水平式胞浆内单精子注射用精子优选方法，其特征在于：采用如权利要求1-4任一项所述的精子优选装置，包括以下步骤：
⑴在精子储存池和优质精子池内加入洗净液，然后开启恒温加热板Ⅰ和恒温加热板Ⅱ；
⑵控制精子储存池内的液体温度为34.5-35.5℃，优质精子池内的液体温度为37.5-38.5℃，将经过梯度离心或者直接离心处理后的精子放入精子储存池内，活力好的精子通过精子游动通道游向优质精子池，从精子放入精子储存池内开始计时，10-20min后，取优质精子池内的精子即为活力好的精子。
CN201810648348.XA 2018-06-22 2018-06-22 水平式胞浆内单精子注射用精子优选装置及精子优选方法 Withdrawn CN108504563A (zh)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4289924A2 (en) 2022-06-08 2023-12-13 iPreg Incorporation Sperm sorting method
EP4289922A1 (en) 2022-06-08 2023-12-13 iPreg Incorporation Vertical thermal gradient equipment and method for progressive sperm sorting

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4289924A2 (en) 2022-06-08 2023-12-13 iPreg Incorporation Sperm sorting method
EP4289922A1 (en) 2022-06-08 2023-12-13 iPreg Incorporation Vertical thermal gradient equipment and method for progressive sperm sorting
EP4289928A2 (en) 2022-06-08 2023-12-13 iPreg Incorporation Sperm sorting device
EP4289924A3 (en) * 2022-06-08 2024-01-17 iPreg Incorporation Sperm sorting method

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