CN108503038A - A kind of zymotechnique of river sewage processing composite bacteria agent - Google Patents
A kind of zymotechnique of river sewage processing composite bacteria agent Download PDFInfo
- Publication number
- CN108503038A CN108503038A CN201810198222.7A CN201810198222A CN108503038A CN 108503038 A CN108503038 A CN 108503038A CN 201810198222 A CN201810198222 A CN 201810198222A CN 108503038 A CN108503038 A CN 108503038A
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- Prior art keywords
- composite bacteria
- bacteria agent
- fermentation
- river sewage
- zymotechnique
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
Abstract
The invention discloses zymotechniques, including nitrobacteria, denitrifying bacteria, photosynthetic bacteria, bacillus subtilis, amino acid ferment bacterium etc. that a kind of river sewage handles composite bacteria agent, which is characterized in that the zymotechnique includes the following steps：Step 1 prepare culture medium, step 2 anaerobic fermentation, step 3 ventilating fermentation, step 4 domestication fermentation, step 5 prepare river sewage processing composite bacteria agent.The present invention is high using nanometer analysis for soybean powder nutritive value, and trace elements can fully keep bioactivity, enhance the nutrition diversity of matrix, is equipped with straw powder and increases content of cellulose, matrix nutrition ingredient is comprehensive, is conducive to the growth of probiotics.Using more wheel sewage and temperature acclimatisation breeding, realize that microbial inoculum flora survival rate and content are high, bioactivity and adaptable, with characteristic that is efficient, stable, quickly breeding, the harmful substances such as ammonia nitrogen in water body of degrading, quickly restore balance water body bacterium phase, purifying water body improves environment.Overcome the deficiencies in the prior art.
Description
Technical field
The present invention relates to the fermentations that a kind of bacteria agent technical field more particularly to a kind of river sewage handle composite bacteria agent
Technique.
Background technology
The river sewage processing composite bacteria agent of the prior art is prepared using normal fermentation technique, fermentation process poor controllability,
Profitable strain survival rate and content are low, bioactivity and bad adaptability.
The present invention is high using breaking cell wall analysis for soybean powder nutritive value, and the nanometer of breaking cell wall is finely pulverized to be made in raw material
Trace elements can fully keep bioactivity, enhance the nutrition diversity of matrix, be equipped with straw powder and increase content of cellulose, matrix
Nutritional ingredient is comprehensive, is conducive to the growth of probiotics.Using more wheel sewage and temperature acclimatisation breeding, microbial inoculum flora survival rate is realized
With content height, bioactivity and adaptable, have efficiently, the characteristic stablizing, quickly breed, have using the disease-resistant decomposition of probiotics
Evil substance, enhances aquatic products healthy growth, the harmful substances such as ammonia nitrogen in water body of degrading, and removal rate is primary to throw up to 97% or more
As long as after putting river water body can be effectively improved within 6 days, algae reproduction is can inhibit, quickly restore balance water body bacterium phase, purifying water body,
Improve environment.Overcome the deficiencies in the prior art.
Invention content
The purpose of the present invention is to provide the zymotechniques that a kind of river sewage handles composite bacteria agent, reasonably solve existing
There is the river sewage processing composite bacteria agent of technology to be prepared using normal fermentation technique, fermentation process poor controllability, profitable strain is deposited
Motility rate and content are low, bioactivity and bad adaptability, cannot fully river training water body the problem of.
The present invention adopts the following technical scheme that：
A kind of zymotechnique of river sewage processing composite bacteria agent, including it is nanometer analysis for soybean powder, straw powder, nitrobacteria, anti-
Nitrobacteria, photosynthetic bacteria, bacillus subtilis, bacillus coagulans, lactic acid bacteria, amino acid ferment bacterium, saccharomycete, feature exist
In,
A kind of zymotechnique of river sewage processing composite bacteria agent includes the following steps：
Step 1: preparing culture medium：Nanometer analysis for soybean powder of the grain size less than 50 nanometers will be crushed to and be crushed to grain size and be less than
The straw powder of 200 mesh presses 1：1 quality is added the 50%-60%'s of the nanometer analysis for soybean powder and straw powder gross mass than mixing
Water prepares culture medium,
Step 2: anaerobic fermentation：The culture medium is moved into and presses 1 in sealed fermenter：1 ratio inoculation nitrobacteria,
Denitrifying bacteria, photosynthetic bacteria, bacillus subtilis, bacillus coagulans, lactic acid bacteria, amino acid ferment bacterium, saccharomycete room temperature are normal
Pressure ferment 96 hours, prepares anaerobic fermentation composite bacteria agent,
Step 3: ventilating fermentation：The culture medium is moved into and presses 1 in ventilating fermentation pond：1 ratio inoculation nitrobacteria,
Denitrifying bacteria, photosynthetic bacteria, bacillus subtilis, bacillus coagulans, lactic acid bacteria, amino acid ferment bacterium, saccharomycete room temperature are normal
Pressure ferment 96 hours, ventilation stirring in every 4 hours 15 minutes, prepares ventilating fermentation composite bacteria agent,
Step 4: domestication fermentation：The anaerobic fermentation composite bacteria agent and the ventilating fermentation composite bacteria agent are merged one
Normal temperature and pressure open culturing is played after 72 hours, the anaerobic fermentation composite bacteria agent is added and the ventilating fermentation composite bacteria agent is overall
Long-pending 1/3 sanitary sewage domestication fermentation 96 hours, adds the anaerobic fermentation composite bacteria agent and the ventilating fermentation is compound
After the 1/3 trade effluent domestication fermentation 96 hours of microbial inoculum total volume, the anaerobic fermentation composite bacteria agent and described logical is added
The 1/3 aquiculture sewerage domestication fermentation of the compoiste fermented microbial inoculum total volume of wind 96 hours, prepares compound domesticated strain agent, described
Domestication fermentation is that normal pressure is open type fermented,
Step 5: preparing river sewage processing composite bacteria agent：The compound domesticated strain agent is pressed 1：1 mass ratio adds
Enter the culture medium, temperature control is 6-8 days open type fermented at 38 DEG C -45 DEG C, prepares river sewage processing composite bacteria agent, constitutes
A kind of zymotechnique of the river sewage processing composite bacteria agent.
Further, the river sewage processing composite bacteria agent includes nitrobacteria, denitrifying bacteria, photosynthetic bacteria, withered grass
Bacillus, bacillus coagulans, lactic acid bacteria, amino acid ferment bacterium, saccharomycete, 4,000,000,000 plants/ml of bacterial strain content.
Further, the temperature control of the domestication fermentation is even with 1 DEG C per hour of pace of change between 5 DEG C -50 DEG C
Fast gradual change cycle.
Further, the river sewage processing composite bacteria agent is 5 DEG C -50 DEG C using adaptive temperature.The present invention's has
Benefit has the technical effect that：
The invention discloses a kind of river sewage handle composite bacteria agent zymotechnique, reasonably solve the prior art
River sewage processing composite bacteria agent using normal fermentation technique prepare, fermentation process poor controllability, profitable strain survival rate with
Content is low, bioactivity and bad adaptability, cannot fully river training water body the problem of.
The present invention is high using breaking cell wall analysis for soybean powder nutritive value, and the nanometer of breaking cell wall is finely pulverized to be made in raw material
Trace elements can fully keep bioactivity, enhance the nutrition diversity of matrix, be equipped with straw powder and increase content of cellulose, matrix
Nutritional ingredient is comprehensive, is conducive to the growth of probiotics.Using more wheel sewage and temperature acclimatisation breeding, microbial inoculum flora survival rate is realized
With content height, bioactivity and adaptable, have efficiently, the characteristic stablizing, quickly breed, have using the disease-resistant decomposition of probiotics
Evil substance, enhances aquatic products healthy growth, the harmful substances such as ammonia nitrogen in water body of degrading, and removal rate is primary to throw up to 97% or more
As long as after putting river water body can be effectively improved within 6 days, algae reproduction is can inhibit, quickly restore balance water body bacterium phase, purifying water body,
Improve environment.Overcome the deficiencies in the prior art.
Specific implementation mode
By the following description of the embodiment, it will more contribute to public understanding of the invention, but can't should be by Shen
Given specific embodiment of asking someone is considered as the limitation to technical solution of the present invention, any definition to component or technical characteristic
Be changed and/or to overall structure make form and immaterial transformation is regarded as defined by technical scheme of the present invention
Protection domain.
Embodiment
A kind of zymotechnique of river sewage processing composite bacteria agent, including it is nanometer analysis for soybean powder, straw powder, nitrobacteria, anti-
Nitrobacteria, photosynthetic bacteria, bacillus subtilis, bacillus coagulans, lactic acid bacteria, amino acid ferment bacterium, saccharomycete, feature exist
In,
A kind of zymotechnique of river sewage processing composite bacteria agent includes the following steps：
Step 1: preparing culture medium：Nanometer analysis for soybean powder of the grain size less than 50 nanometers will be crushed to and be crushed to grain size and be less than
The straw powder of 200 mesh presses 1：1 quality is added the 50%-60%'s of the nanometer analysis for soybean powder and straw powder gross mass than mixing
Water prepares culture medium,
Step 2: anaerobic fermentation：The culture medium is moved into and presses 1 in sealed fermenter：1 ratio inoculation nitrobacteria,
Denitrifying bacteria, photosynthetic bacteria, bacillus subtilis, bacillus coagulans, lactic acid bacteria, amino acid ferment bacterium, saccharomycete room temperature are normal
Pressure ferment 96 hours, prepares anaerobic fermentation composite bacteria agent,
Step 3: ventilating fermentation：The culture medium is moved into and presses 1 in ventilating fermentation pond：1 ratio inoculation nitrobacteria,
Denitrifying bacteria, photosynthetic bacteria, bacillus subtilis, bacillus coagulans, lactic acid bacteria, amino acid ferment bacterium, saccharomycete room temperature are normal
Pressure ferment 96 hours, ventilation stirring in every 4 hours 15 minutes, prepares ventilating fermentation composite bacteria agent,
Step 4: domestication fermentation：The anaerobic fermentation composite bacteria agent and the ventilating fermentation composite bacteria agent are merged one
Normal temperature and pressure open culturing is played after 72 hours, the anaerobic fermentation composite bacteria agent is added and the ventilating fermentation composite bacteria agent is overall
Long-pending 1/3 sanitary sewage domestication fermentation 96 hours, adds the anaerobic fermentation composite bacteria agent and the ventilating fermentation is compound
After the 1/3 trade effluent domestication fermentation 96 hours of microbial inoculum total volume, the anaerobic fermentation composite bacteria agent and described logical is added
The 1/3 aquiculture sewerage domestication fermentation of the compoiste fermented microbial inoculum total volume of wind 96 hours, prepares compound domesticated strain agent, described
Domestication fermentation is that normal pressure is open type fermented,
Step 5: preparing river sewage processing composite bacteria agent：The compound domesticated strain agent is pressed 1：1 mass ratio adds
Enter the culture medium, temperature control is 6-8 days open type fermented at 38 DEG C -45 DEG C, prepares river sewage processing composite bacteria agent.It completes
A kind of implementation of the zymotechnique of the river sewage processing composite bacteria agent.
Certainly, the present invention can also have other various embodiments, without deviating from the spirit and substance of the present invention,
Those skilled in the art can make various corresponding change and deformations according to the present invention, but these it is corresponding change and
Deformation should all belong to the protection domain of appended claims of the invention.
Claims (4)
1. a kind of zymotechnique of river sewage processing composite bacteria agent, including nanometer analysis for soybean powder, straw powder, nitrobacteria, anti-nitre
Change bacterium, photosynthetic bacteria, bacillus subtilis, bacillus coagulans, lactic acid bacteria, amino acid ferment bacterium, saccharomycete, feature exists
In,
A kind of zymotechnique of river sewage processing composite bacteria agent includes the following steps：
Step 1: preparing culture medium：Nanometer analysis for soybean powder of the grain size less than 50 nanometers will be crushed to and be crushed to grain size and be less than 200 mesh
Straw powder press 1：For 1 quality than mixing, prepared by the water that the 50%-60% of the nanometer analysis for soybean powder and straw powder gross mass is added
Culture medium,
Step 2: anaerobic fermentation：The culture medium is moved into and presses 1 in sealed fermenter：1 ratio is inoculated with nitrobacteria, anti-nitre
Change bacterium, photosynthetic bacteria, bacillus subtilis, bacillus coagulans, lactic acid bacteria, amino acid ferment bacterium, saccharomycete normal temperature and pressure hair
Ferment 96 hours, prepares anaerobic fermentation composite bacteria agent,
Step 3: ventilating fermentation：The culture medium is moved into and presses 1 in ventilating fermentation pond：1 ratio is inoculated with nitrobacteria, anti-nitre
Change bacterium, photosynthetic bacteria, bacillus subtilis, bacillus coagulans, lactic acid bacteria, amino acid ferment bacterium, saccharomycete normal temperature and pressure hair
Ferment 96 hours, ventilation stirring in every 4 hours 15 minutes, prepares ventilating fermentation composite bacteria agent,
Step 4: domestication fermentation：The anaerobic fermentation composite bacteria agent and the ventilating fermentation composite bacteria agent are merged often
After normal temperature and pressure open culturing 72 hours, the anaerobic fermentation composite bacteria agent and the ventilating fermentation composite bacteria agent total volume is added
1/3 sanitary sewage domestication fermentation 96 hours, adds the anaerobic fermentation composite bacteria agent and the ventilating fermentation composite bacteria agent
After the 1/3 trade effluent domestication of total volume is fermented 96 hours, the anaerobic fermentation composite bacteria agent and ventilation hair are added
The 1/3 aquiculture sewerage domestication fermentation of ferment composite bacteria agent total volume 96 hours, prepares compound domesticated strain agent, the domestication
Fermentation is that normal pressure is open type fermented,
Step 5: preparing river sewage processing composite bacteria agent：The compound domesticated strain agent is pressed 1：Described in 1 mass ratio is added
Culture medium, temperature control is 6-8 days open type fermented at 38 DEG C -45 DEG C, prepares river sewage processing composite bacteria agent, constitutes described one
The zymotechnique of kind river sewage processing composite bacteria agent.
2. a kind of zymotechnique of river sewage processing composite bacteria agent according to claim 1, which is characterized in that the river
Sewage disposal composite bacteria agent includes nitrobacteria, denitrifying bacteria, photosynthetic bacteria, bacillus subtilis, bacillus coagulans, lactic acid
Bacterium, amino acid ferment bacterium, saccharomycete, 4,000,000,000 plants/ml of bacterial strain content.
3. a kind of zymotechnique of river sewage processing composite bacteria agent according to claim 1, which is characterized in that the domestication
The temperature control of fermentation is recycled between being 5 DEG C -50 DEG C with the at the uniform velocity gradual change of 1 DEG C per hour of pace of change.
4. a kind of zymotechnique of river sewage processing composite bacteria agent according to claim 1, which is characterized in that the river
Sewage disposal composite bacteria agent is 5 DEG C -50 DEG C using adaptive temperature.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810198222.7A CN108503038A (en) | 2018-03-09 | 2018-03-09 | A kind of zymotechnique of river sewage processing composite bacteria agent |
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| CN201810198222.7A CN108503038A (en) | 2018-03-09 | 2018-03-09 | A kind of zymotechnique of river sewage processing composite bacteria agent |
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| CN108503038A true CN108503038A (en) | 2018-09-07 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113322203A (en) * | 2021-05-31 | 2021-08-31 | 青岛万慧源环保科技有限公司 | Composite microbial inoculum for aerobic denitrification and preparation method thereof |
| CN113564088A (en) * | 2021-09-16 | 2021-10-29 | 张相润 | Culture method for reducing eutrophication strains in aquaculture |
| US11629083B2 (en) * | 2019-10-31 | 2023-04-18 | Tongji University | Organic composite powder carrier and application thereof in enhanced biological denitrification of municipal wastewater treatment |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2364955A1 (en) * | 2008-12-10 | 2011-09-14 | Takurou Fujita | Nitrate-removing bioreactor, method for forming nitrate-removing biofilm, and nitrate-removing method |
| CN102250768A (en) * | 2011-06-01 | 2011-11-23 | 康源绿洲微生物技术（北京）有限公司 | Method for preparing enzyme and bacterium composite agent for treating sewage and sludge |
| CN106701633A (en) * | 2017-01-22 | 2017-05-24 | 哈尔滨明慧生物技术开发有限公司 | Compound bactericide for treating sewage and sewage treatment method thereof |
-
2018
- 2018-03-09 CN CN201810198222.7A patent/CN108503038A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2364955A1 (en) * | 2008-12-10 | 2011-09-14 | Takurou Fujita | Nitrate-removing bioreactor, method for forming nitrate-removing biofilm, and nitrate-removing method |
| CN102250768A (en) * | 2011-06-01 | 2011-11-23 | 康源绿洲微生物技术（北京）有限公司 | Method for preparing enzyme and bacterium composite agent for treating sewage and sludge |
| CN106701633A (en) * | 2017-01-22 | 2017-05-24 | 哈尔滨明慧生物技术开发有限公司 | Compound bactericide for treating sewage and sewage treatment method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11629083B2 (en) * | 2019-10-31 | 2023-04-18 | Tongji University | Organic composite powder carrier and application thereof in enhanced biological denitrification of municipal wastewater treatment |
| CN113322203A (en) * | 2021-05-31 | 2021-08-31 | 青岛万慧源环保科技有限公司 | Composite microbial inoculum for aerobic denitrification and preparation method thereof |
| CN113564088A (en) * | 2021-09-16 | 2021-10-29 | 张相润 | Culture method for reducing eutrophication strains in aquaculture |
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Application publication date: 20180907 |