CN108489780A - One kind is for carrying out the fixed device of chemistry under high pressure - Google Patents
One kind is for carrying out the fixed device of chemistry under high pressure Download PDFInfo
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- CN108489780A CN108489780A CN201810175173.5A CN201810175173A CN108489780A CN 108489780 A CN108489780 A CN 108489780A CN 201810175173 A CN201810175173 A CN 201810175173A CN 108489780 A CN108489780 A CN 108489780A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention discloses one kind for carrying out the fixed device of chemistry under high pressure, the first syringe, the second syringe including autoclave and in the autoclave and the first pressure limiting valve, first syringe and the second syringe are interconnected by first pressure limiting valve, first syringe, the second syringe are equipped with for changing the first piston of syringe volume, and the first piston moves under the promotion of dynamical system.It is fixed for realizing chemistry is carried out under high pressure, solve the technical barrier that sample liquid and fixer mixed proportion are accurately controlled under high pressure.
Description
Technical field
It is the present invention relates to chemical fixed equipment technical field, more particularly to a kind of for carrying out the fixed dress of chemistry under high pressure
It sets.
Background technology
Pressure is an important thermodynamic parameter, it has shadow to many aspects of microbial cell and large biological molecule
It rings.Under condition of high voltage, cell cannot carry out proper splitting, depolymerization can occur for the polymer of large biological molecule, albumen it is advanced
Structure and conformation change.And high pressure caused by microbial cell and large biological molecule these influence often it is instantaneous, can
Inverse.When ambient pressure reduces, the Physiology and biochemistry that microbial cell and large biological molecule are shown under high pressure is special
Property can also change therewith.Therefore, how will be under the in stable condition preservation of the physics and chemistry of cell or large biological molecule under high pressure
It is always to hinder a main cause of high pressure biology progress to carry out subsequent analysis.Before this, Japanese researchers once design
A kind of high pressure fixing device has been made, and has been applied to influence of the analysis pressure to cellular morphology and property of protein.The device
It is sample liquid and fixer to be individually positioned in two test tubes, and two test tubes are connected, centre sheet glass or close
Sealer separates, wherein being placed with weight with a needle in the test tube equipped with fixer.Whole device is put into autoclave, is added
Pressure.When needing to be fixed under high pressure, by the autoclave that turns upside down, middle interval is poked by built-in weight
Film realizes liquid mixing, it is fixed to carry out chemistry.There are many places disadvantage, 1, sample liquid and fixations for this device used at present
When liquid mixes, the ratio of the two cannot be accurately controlled, causes the fixed sample of different batches, quality inhomogenous；2, in operation
It needs repeatedly to be sealed test tube with diaphragm seal, it is time-consuming longer, it is cumbersome；3, due to resistance that weight is subject in a liquid
It is larger, turn upside down autoclave when often there is diaphragm seal or the case where sheet glass is not easy to poke, lead to experiment success rate
It is not high；Operator is needed repeatedly to overturn heavier autoclave when 4, mixing, there are potential insecurity.Therefore, it is necessary to more
Safe efficient, accurate high pressure fixing device.
Invention content
For the above-mentioned prior art, the technical problem to be solved in the present invention is to provide a kind of for carrying out chemistry under high pressure
Fixed device, it is fixed for realizing chemistry is carried out under high pressure, it solves and accurately controls sample liquid and fixation under high pressure
The technical barrier of liquid mixed proportion.
One kind is for carrying out the fixed device of chemistry, including autoclave and the first note in the autoclave under high pressure
Emitter, the second syringe and the first pressure limiting valve, first syringe and the second syringe are mutual by first pressure limiting valve
Connection, first syringe, the second syringe are equipped with for changing the first piston of syringe volume, the first piston
It is moved under the promotion of dynamical system.
Further, first pressure limiting valve includes elastic hose and plug pearl, and the elastic hose both ends are separately connected institute
The first syringe and second syringe are stated, the plug pearl is set in the elastic hose, and the plug pearl diameter is more than described
Elastic hose internal diameter.
Further, the elastic hose is silica gel hose, and the plug pearl is stainless steel steel ball.
Further, the autoclave is connected with the second pressure limiting valve and pressure gauge, and the pressure gauge is for detecting autoclave
The water inlet end of interior pressure, second pressure limiting valve is connected to the autoclave, between second pressure limiting valve and the autoclave
Equipped with the first inlet.
Further, the water outlet of second pressure limiting valve is connected to waste liquid cylinder.
Further, the waste liquid cylinder is equipped with scale.
Further, the dynamical system includes second piston and force (forcing) pump, and the second piston is in the autoclave
Activity and autoclave is divided into the first vessel and the second vessel, first syringe and the second syringe are set to described second
In vessel, the force (forcing) pump is connected to first vessel.
Further, further include water receiver, the force (forcing) pump is connect with the water receiver.
Further, fixed frame is equipped in the autoclave, first syringe and second syringe are fixed
On the fixed frame.
Further, the fixed frame is equipped with screw rod and nut, and the nut cooperates with the screw rod, the nut
For first syringe and second syringe to be fixed.
The beneficial effects of the present invention are：Autoclave provides hyperbaric environment for reaction, can be used for carrying out under high pressure
The in-situ chemical of cell or protein sample is fixed, and the repeatability of experiment can be more effectively ensured in more existing system；The system knot
Structure is simple, and technology is reasonable.It is packed into fixer in the first syringe, is packed into sample liquid in the second syringe, and passes through the first limit
Pressure valve is interconnected, and improves fixer and the mode of communicating of sample liquid, improves experiment success rate.The device is to pass through movement
Piston realizes liquid proportional mixing, has ensured the accuracy and repeatability of experiment.Moreover, need not be overturned in mixed process
Heavier autoclave improves the safety of operation.
Description of the drawings
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, the accompanying drawings in the following description is only the preferred embodiment of the present invention, for
For those of ordinary skill in the art, without having to pay creative labor, it can also be obtained according to these attached drawings
His attached drawing.
Fig. 1 is one kind of the present invention for carrying out the fixed apparatus structure schematic diagram of chemistry under high pressure；
Fig. 2 is the autoclave structural schematic diagram of the present invention；
In figure, 1 autoclave, 2 first syringes, 3 second syringes, 4 first pressure limiting valves, 5 first pistons, 6 elastic hoses,
7 plug pearls, 8 second pressure limiting valves, 9 pressure gauges, 10 first inlets, 11 waste liquid cylinders, 12 scales, 13 second pistons, 14 force (forcing) pumps, 15
First vessel, 16 second vessels, 17 water receivers, 18 fixed frames, 19 screw rods, 20 nuts.
Specific implementation mode
In order to be best understood from the technology of the present invention content, be provided below specific embodiment, and in conjunction with attached drawing to the present invention do into
The explanation of one step.
Embodiment 1
Referring to Fig. 1 and Fig. 2, a kind of for carrying out the fixed device of chemistry under high pressure, including autoclave 1 and it is set to the height
Press the first syringe 2, the second syringe 3 and the first pressure limiting valve 4 in kettle 1, first syringe, 2 and second syringe 3 logical
It crosses first pressure limiting valve 4 to be interconnected, first syringe 2, the second syringe 3 are equipped with for changing syringe volume
First piston 5, the first piston 5 moves under the promotion of dynamical system.It is fixed specifically, being packed into the first syringe 2
Liquid, the second syringe 3 are packed into sample liquid, inject highly pressurised liquid into autoclave 1, since first piston 5 is movable, reach flat
The pressure in pressure, the second syringe 3 when weighing apparatus in the first syringe 2 is identical with the pressure three in autoclave 1, and at this time
One pressure limiting valve 4 is not turned on, and sample liquid and fixer are separated from each other, and sample liquid is made to be reacted under high pressure, multigroup cell or
Protein sample is in hyperbaric environment but not by the fixed state of carry out in-situ chemical.It waits after the completion of reacting, is pushed away by dynamical system
Dynamic first piston 5 moves certain distance, and further, dynamical system pushes the first work in the first syringe 2 equipped with fixer
Plug 5, the first piston 5 that the second syringe 3 of sample liquid is housed at this time do not move.First piston 5 in first syringe 2 moves
Pressure balance is destroyed afterwards, and pressure in the first syringe 2 increases, and the first pressure limiting valve 4 is opened, and fixer is from the first syringe
2 mix into the second syringe 3 with sample liquid, and with the injection of fixer, the first piston 5 in the second syringe 3 starts to move
It is dynamic, it is final to realize that multigroup cell or protein sample, which are carried out in-situ chemical, to be fixed convenient for maintaining the balance of pressure in syringe.
Specifically, first pressure limiting valve 4 includes elastic hose 6 and plug pearl 7,6 both ends of the elastic hose are separately connected
First syringe 2 and second syringe 3, the plug pearl 7 are set in the elastic hose 6, and 7 diameters of the plug pearl are big
In 6 internal diameter of the elastic hose.Preferably, the elastic hose 6 is silica gel hose, and the plug pearl 7 is stainless steel steel ball.Pass through
The silica gel hose for being built-in with stainless steel steel ball connects two syringes, realizes two solution under high pressure mutually isolated.
By pushing first piston 5 to pressurize when needing, so that silica gel hose is expanded, release the buffer action of steel ball, realize the liquid under high pressure
Body connection mixing.Stainless steel steel ball is arranged in silica gel hose two syringes are isolated, it is simple in structure, it is of low cost,
The pressure being subject to inside and outside silica gel hose in autoclave 1 is identical, and silica gel hose does not deform upon.Dynamical system pushes first piston
When 5 in first syringe 2 pressure be more than 3 pressure of autoclave 1 and the second syringe, so that silica gel hose is expanded, release steel ball every
From effect, the fluid connection mixing under high pressure is realized.Elastic hose 6 and plug pearl 7 are small, are convenient for the first syringe 2 and second
The installation and removal of syringe 3.Silica gel hose 6 is shunk after injection, moreover it is possible to obstruct the channel of two syringes.
Specifically, the dynamical system includes second piston 13 and force (forcing) pump 14, the second piston 13 is in the high pressure
In kettle 1 activity and autoclave 1 is divided into the first vessel 15 and the second vessel 16, first syringe, 2 and second syringe 3
In second vessel 16, the force (forcing) pump 14 is connected to first vessel 15.Force (forcing) pump 14 is into the first vessel 15
Pumping liquid, as the increase second piston 13 of pressure is moved to the second vessel 16 and is contacted with first piston 5, with the second work
Plug 13 continuing to move to and push first piston 5 squeeze the first syringe 2 in fixer, fixer pressure increase, make silica gel
Hose expansion releases the buffer action of stainless steel steel ball, and fixer is injected into sample liquid and mixes, and realizes that chemistry is solid under high pressure
It is fixed.Force (forcing) pump 14 can control the pressure of whole system.The force (forcing) pump 14 can pressurize within the scope of 0.1-60MPa, simultaneously
Compression rate can be set, the mixing rate for adjusting sample liquid and fixer.
Specifically, further including water receiver 17, the force (forcing) pump 14 is connect with the water receiver 17.Water receiver 17 is for storing
Distilled water, for providing pressure medium, the water in water receiver 17 is pumped into the first vessel 15 by water pump.
Embodiment 2
Referring to Fig. 1 and Fig. 2, difference lies in the autoclave 1 is connected with the second pressure limiting valve 8 to the present embodiment with embodiment 1
With pressure gauge 9, the pressure gauge 9 is for detecting pressure in autoclave 1, water inlet end and the height of second pressure limiting valve 8
It presses kettle 1 to be connected to, the first inlet 10 is equipped between second pressure limiting valve 8 and the autoclave 1.Pressure gauge 9 is for detecting height
Pressure in kettle 1 is pressed, due to the second syringe 3 and 1 pressure balance of autoclave, i.e., pressure is equal in the second syringe 3 in autoclave 1
The pressure of sample liquid, therefore the pressure in 9 the second syringe of readable 3 of pressure gauge, convenient for the pressure change of monitoring whole system.
Second pressure limiting valve 8 is used to maintain the pressure stability of whole system, also plays the role of protection to device.
Further, the water outlet connection waste liquid cylinder 11 of second pressure limiting valve 8.Second pressure limiting valve when pressure is more than threshold value
8 open, and water is expelled to from the water outlet of the second pressure limiting valve 8 in waste liquid cylinder 11, and waste liquid cylinder 11 collects water, avoids wasting water
Resource, collected water is repeatable to be utilized.
Further, the waste liquid cylinder 11 is equipped with scale 12.With a scale 12 can show discharge liquid volume, lead to
Cross the volume of trickle in observation waste liquid cylinder 11, it can be determined that the injection rate of fixer, the mixed proportion both to control.
Embodiment 3
Referring to Fig. 1 and Fig. 2, the present embodiment the difference from example 2 is that, fixed frame 18, institute are equipped in the autoclave 1
It states the first syringe 2 and second syringe 3 is each attached on the fixed frame 18.Fixed frame 18 can be fixed equipped with fixation
First syringe 2 of liquid and the second syringe 3 equipped with sample liquid, further, the fixing bracket are set there are three fixed bit,
Three groups of experiments can be carried out at the same time.
Specifically, the fixed frame 18 is equipped with screw rod 19 and nut 20, the nut 20 cooperates with the screw rod 19,
The nut 20 is for fixing first syringe 2 and second syringe 3.It can be according to the specification of syringe used
It is adjusted so that device can adapt to the syringe of different size.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (10)
1. one kind is for carrying out the fixed device of chemistry under high pressure, which is characterized in that including autoclave and be set to the autoclave
Interior the first syringe, the second syringe and the first pressure limiting valve, first syringe and the second syringe pass through described first
Pressure limiting valve is interconnected, and first syringe, the second syringe are equipped with for changing the first piston of syringe volume, institute
First piston is stated to move under the promotion of dynamical system.
2. according to claim 1 a kind of for carrying out the fixed device of chemistry under high pressure, which is characterized in that described first
Pressure limiting valve includes elastic hose and plug pearl, and the elastic hose both ends are separately connected first syringe and second injection
Device, the plug pearl are set in the elastic hose, and the plug pearl diameter is more than the elastic hose internal diameter.
3. according to claim 2 a kind of for carrying out the fixed device of chemistry under high pressure, which is characterized in that the elasticity
Hose is silica gel hose, and the plug pearl is stainless steel steel ball.
4. according to claim 1 a kind of for carrying out the fixed device of chemistry under high pressure, which is characterized in that the high pressure
Kettle is connected with the second pressure limiting valve and pressure gauge, the pressure gauge for detecting pressure in autoclave, second pressure limiting valve into
Water end (W.E.) is connected to the autoclave, and the first inlet is equipped between second pressure limiting valve and the autoclave.
5. according to claim 4 a kind of for carrying out the fixed device of chemistry under high pressure, which is characterized in that described second
The water outlet of pressure limiting valve is connected to waste liquid cylinder.
6. according to claim 5 a kind of for carrying out the fixed device of chemistry under high pressure, which is characterized in that the waste liquid
Cylinder is equipped with scale.
7. according to claim 1 a kind of for carrying out the fixed device of chemistry under high pressure, which is characterized in that the power
System includes second piston and force (forcing) pump, and the second piston is movable in the autoclave and autoclave is divided into the first appearance
Chamber and the second vessel, first syringe and the second syringe are set in second vessel, the force (forcing) pump and described the
One vessel is connected to.
8. according to claim 7 a kind of for carrying out the fixed device of chemistry under high pressure, which is characterized in that further include storage
Hydrophone, the force (forcing) pump are connect with the water receiver.
9. according to claim 1 a kind of for carrying out the fixed device of chemistry under high pressure, which is characterized in that the high pressure
Fixed frame is equipped in kettle, first syringe and second syringe are each attached on the fixed frame.
10. according to claim 9 a kind of for carrying out the fixed device of chemistry under high pressure, which is characterized in that described solid
Surely be provided with screw rod and nut, the nut cooperates with the screw rod, the nut be used for will first syringe with
Second syringe is fixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810175173.5A CN108489780B (en) | 2018-03-02 | 2018-03-02 | Device for chemical fixation under high pressure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810175173.5A CN108489780B (en) | 2018-03-02 | 2018-03-02 | Device for chemical fixation under high pressure |
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| Publication Number | Publication Date |
|---|---|
| CN108489780A true CN108489780A (en) | 2018-09-04 |
| CN108489780B CN108489780B (en) | 2021-04-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810175173.5A Active CN108489780B (en) | 2018-03-02 | 2018-03-02 | Device for chemical fixation under high pressure |
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