CN108489780B - Device for chemical fixation under high pressure - Google Patents
Device for chemical fixation under high pressure Download PDFInfo
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- CN108489780B CN108489780B CN201810175173.5A CN201810175173A CN108489780B CN 108489780 B CN108489780 B CN 108489780B CN 201810175173 A CN201810175173 A CN 201810175173A CN 108489780 B CN108489780 B CN 108489780B
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- pressure
- injector
- autoclave
- limiting valve
- syringe
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Abstract
The invention discloses a device for carrying out chemical fixation under high pressure, which comprises an autoclave, and a first injector, a second injector and a first pressure limiting valve which are arranged in the autoclave, wherein the first injector and the second injector are mutually communicated through the first pressure limiting valve, the first injector and the second injector are respectively provided with a first piston for changing the volume of the injectors, and the first piston moves under the pushing of a power system. The method is used for realizing chemical fixation under high pressure, and solves the technical problem of accurately controlling the mixing ratio of the sample liquid and the fixing liquid under high pressure.
Description
Technical Field
The invention relates to the technical field of chemical fixing equipment, in particular to a device for carrying out chemical fixing under high pressure.
Background
Pressure is an important thermodynamic parameter that has an effect on many aspects of microbial cells and biomacromolecules. For example, under high pressure, cells can not normally divide, polymers of biological macromolecules are depolymerized, and the higher structure and conformation of the protein are changed. These effects of high pressure on microbial cells and biomacromolecules tend to be transient and reversible. When the external pressure is reduced, the physiological and biochemical characteristics of the microbial cells and the biomacromolecules under the high-pressure condition are changed. Therefore, how to stably store the physicochemical state of cells or biomacromolecules under high pressure for subsequent analysis has been a main reason for hindering the development of high pressure biology. Heretofore, Japanese researchers have designed and fabricated a high pressure fixation device and applied it to analyze the effect of pressure on cell morphology and protein properties. The device is characterized in that sample liquid and stationary liquid are respectively placed in two test tubes and are connected, the middle of the two test tubes is separated by a glass sheet or a sealing film, and a weight with a needle is placed in the test tube filled with the stationary liquid. The whole apparatus was placed in an autoclave and pressurized. When the fixing is required under the high-pressure condition, the autoclave is inverted up and down, and the middle diaphragm is punctured by the aid of a built-in weight, so that liquid mixing is realized, and chemical fixing is performed. The currently used device has a plurality of defects, 1, when the sample liquid and the stationary liquid are mixed, the proportion of the sample liquid and the stationary liquid cannot be accurately controlled, so that fixed samples in different batches have uneven quality; 2. the test tube needs to be sealed by a sealing film for multiple times during operation, so that the time is long, and the operation is complicated; 3. because the resistance of the weight in the liquid is large, the situation that a sealing film or a glass sheet is not easy to puncture when the autoclave is inverted up and down often exists, so that the success rate of the experiment is not high; 4. the heavier autoclave is required to be inverted several times by the operator during mixing, with potential unsafe factors. Therefore, there is a need for a safer, more efficient, and more accurate high pressure fixation device.
Disclosure of Invention
In view of the above prior art, the technical problem to be solved by the present invention is to provide a device for chemical fixation under high pressure, which is used for implementing chemical fixation under high pressure, and solves the technical problem of accurately controlling the mixing ratio of sample liquid and fixation liquid under high pressure.
A device for carrying out chemical fixation under high pressure comprises an autoclave, and a first injector, a second injector and a first pressure limiting valve which are arranged in the autoclave, wherein the first injector and the second injector are communicated with each other through the first pressure limiting valve, the first injector and the second injector are respectively provided with a first piston for changing the volume of the injectors, and the first piston moves under the pushing of a power system.
Furthermore, the first pressure limiting valve comprises an elastic hose and a plug bead, two ends of the elastic hose are respectively connected with the first injector and the second injector, the plug bead is arranged in the elastic hose, and the diameter of the plug bead is larger than the inner diameter of the elastic hose.
Furthermore, the elastic hose is a silica gel hose, and the plug beads are stainless steel balls.
Furthermore, the autoclave is connected with a second pressure limiting valve and a pressure gauge, the pressure gauge is used for detecting the pressure in the autoclave, the water inlet end of the second pressure limiting valve is communicated with the autoclave, and a first liquid inlet is arranged between the second pressure limiting valve and the autoclave.
Furthermore, the water outlet end of the second pressure limiting valve is communicated with the waste liquid cylinder.
Furthermore, scales are arranged on the waste liquid cylinder.
Furthermore, the power system comprises a second piston and a pressure pump, the second piston moves in the high-pressure kettle and divides the high-pressure kettle into a first containing cavity and a second containing cavity, the first injector and the second injector are arranged in the second containing cavity, and the pressure pump is communicated with the first containing cavity.
Further, the device also comprises a water storage tank, and the pressurizing pump is connected with the water storage tank.
Furthermore, a fixing frame is arranged in the autoclave, and the first injector and the second injector are both fixed on the fixing frame.
Furthermore, the fixed frame is provided with a screw rod and a nut, the nut is matched with the screw rod, and the nut is used for fixing the first injector and the second injector.
The invention has the beneficial effects that: the high-pressure autoclave provides a high-pressure environment for reaction, can be used for carrying out in-situ chemical fixation on cells or protein samples under a high-pressure condition, and can effectively ensure the repeatability of an experiment compared with the conventional system; the system has simple structure and reasonable technology. The first syringe is filled with the stationary liquid, the second syringe is filled with the sample liquid, and the stationary liquid and the sample liquid are communicated with each other through the first pressure limiting valve, so that the communication mode of the stationary liquid and the sample liquid is improved, and the success rate of the experiment is improved. The device realizes the proportional mixing of liquid by moving the piston, and ensures the accuracy and repeatability of the experiment. Moreover, a heavier autoclave does not need to be inverted in the mixing process, and the operation safety is improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only preferred embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive exercise.
FIG. 1 is a schematic diagram of an apparatus for chemical fixation at high pressure according to the present invention;
FIG. 2 is a schematic diagram of the structure of an autoclave according to the present invention;
in the figure, 1 autoclave, 2 first injector, 3 second injector, 4 first pressure limiting valve, 5 first piston, 6 elastic hose, 7 stopple, 8 second pressure limiting valve, 9 pressure gauge, 10 first liquid inlet, 11 waste liquid cylinder, 12 scale, 13 second piston, 14 pressure pump, 15 first containing cavity, 16 second containing cavity, 17 water receiver, 18 fixing frame, 19 screw rod, 20 nut.
Detailed Description
In order to better understand the technical content of the invention, specific embodiments are provided below, and the invention is further described with reference to the accompanying drawings.
Example 1
Referring to fig. 1 and 2, the device for chemical fixation under high pressure comprises an autoclave 1, and a first injector 2, a second injector 3 and a first pressure limiting valve 4 which are arranged in the autoclave 1, wherein the first injector 2 and the second injector 3 are communicated with each other through the first pressure limiting valve 4, the first injector 2 and the second injector 3 are both provided with a first piston 5 for changing the volume of the injectors, and the first piston 5 moves under the pushing of a power system. Specifically, a first syringe 2 is filled with a fixed liquid, a second syringe 3 is filled with a sample liquid, a high-pressure liquid is injected into the autoclave 1, and due to the fact that a first piston 5 is movable, when equilibrium is achieved, the pressure in the first syringe 2, the pressure in the second syringe 3 and the pressure in the autoclave 1 are the same, at the moment, a first pressure limiting valve 4 is not opened, the sample liquid and the fixed liquid are separated from each other, the sample liquid is enabled to react under high pressure, and a plurality of groups of cell or protein samples are in a high-pressure environment but are not subjected to in-situ chemical fixation. After the reaction is finished, the power system pushes the first piston 5 to move for a certain distance, and further, the power system pushes the first piston 5 in the first syringe 2 filled with the stationary liquid, and at the moment, the first piston 5 of the second syringe 3 filled with the sample liquid does not move. After the first piston 5 in the first syringe 2 moves, the pressure balance is broken, the pressure in the first syringe 2 increases, the first pressure limiting valve 4 is opened, the fixing liquid enters the second syringe 3 from the first syringe 2 to be mixed with the sample liquid, and along with the injection of the fixing liquid, the first piston 5 in the second syringe 3 starts to move, so that the balance of the pressure in the syringes is maintained, and finally, the in-situ chemical fixation of a plurality of groups of cell or protein samples is realized.
Specifically, the first pressure limiting valve 4 comprises an elastic hose 6 and a plug bead 7, two ends of the elastic hose 6 are respectively connected with the first syringe 2 and the second syringe 3, the plug bead 7 is arranged in the elastic hose 6, and the diameter of the plug bead 7 is larger than the inner diameter of the elastic hose 6. Preferably, the elastic hose 6 is a silica gel hose, and the plug bead 7 is a stainless steel ball. The two injectors are connected through a silica gel hose with built-in stainless steel balls, so that the two solutions are isolated from each other under a high-pressure condition. When in need, the first piston 5 is pushed to pressurize, so that the silica gel hose expands, the isolation effect of the steel balls is removed, and liquid communication and mixing under high pressure are realized. The stainless steel balls are arranged in the silica gel hose to isolate the two injectors, the structure is simple, the cost is low, the pressure inside and outside the silica gel hose in the high-pressure kettle 1 is the same, and the silica gel hose is not deformed. When the power system pushes the first piston 5, the pressure in the first injector 2 is greater than the pressure in the autoclave 1 and the second injector 3, so that the silica gel hose expands, the isolation effect of the steel balls is relieved, and liquid under high pressure is communicated and mixed. The elastic hose 6 and the plug bead 7 are small in size, and the first syringe 2 and the second syringe 3 can be conveniently mounted and dismounted. After the injection is finished, the silica gel hose 6 shrinks, and the channels of the two injectors can be blocked.
Specifically, the power system comprises a second piston 13 and a pressure pump 14, the second piston 13 moves in the autoclave 1 and divides the autoclave 1 into a first containing cavity 15 and a second containing cavity 16, the first injector 2 and the second injector 3 are arranged in the second containing cavity 16, and the pressure pump 14 is communicated with the first containing cavity 15. The pressurizing pump 14 pumps liquid into the first containing cavity 15, the second piston 13 moves towards the second containing cavity 16 along with the increase of pressure and is in contact with the first piston 5, the first piston 5 is pushed to extrude fixing liquid in the first injector 2 along with the continuous movement of the second piston 13, the fixing liquid pressure is increased, the silica gel hose is expanded, the isolation effect of the stainless steel ball is relieved, the fixing liquid is injected into the sample liquid to be mixed, and chemical fixation under high pressure is realized. The booster pump 14 can control the pressure of the entire system. The pressurizing pump 14 can pressurize in the range of 0.1 to 60MPa, and the pressurizing rate can be set for adjusting the mixing rate of the sample liquid and the fixation liquid.
Specifically, a water reservoir 17 is further included, and the pressure pump 14 is connected to the water reservoir 17. The reservoir 17 is intended to store distilled water and to provide a pressurized medium, and the water pump pumps the water in the reservoir 17 into the first chamber 15.
Example 2
Referring to fig. 1 and fig. 2, the present embodiment is different from embodiment 1 in that a second pressure-limiting valve 8 and a pressure gauge 9 are connected to the autoclave 1, the pressure gauge 9 is used for detecting the pressure in the autoclave 1, a water inlet end of the second pressure-limiting valve 8 is communicated with the autoclave 1, and a first liquid inlet 10 is arranged between the second pressure-limiting valve 8 and the autoclave 1. The pressure gauge 9 is used for detecting the pressure in the autoclave 1, and since the pressure in the second syringe 3 is balanced with the pressure in the autoclave 1, namely the pressure in the autoclave 1 is equal to the pressure of the sample liquid in the second syringe 3, the pressure gauge 9 can read the pressure in the second syringe 3, thereby being convenient for monitoring the pressure change of the whole system. The second pressure limiting valve 8 is used for maintaining the pressure stability of the whole system and also plays a role in protecting the device.
Further, the water outlet end of the second pressure limiting valve 8 is communicated with a waste liquid cylinder 11. When the pressure exceeds the threshold value, the second pressure limiting valve 8 is opened, water is discharged from the water outlet end of the second pressure limiting valve 8 to the waste liquid cylinder 11, the waste liquid cylinder 11 collects the water, water resources are prevented from being wasted, and the collected water can be recycled.
Furthermore, the waste liquid cylinder 11 is provided with a scale 12. The scale 12 can display the volume of the discharged liquid, and the injection amount of the fixation liquid can be judged by observing the volume of the discharged liquid in the waste liquid tank 11, thereby controlling the mixing ratio of the two.
Example 3
Referring to fig. 1 and 2, the present embodiment is different from embodiment 2 in that a fixing frame 18 is provided in the autoclave 1, and the first injector 2 and the second injector 3 are fixed to the fixing frame 18. The first syringe 2 that is equipped with stationary liquid and the second syringe 3 that is equipped with sample liquid can be fixed to mount 18, and is further, the fixed bolster is equipped with three fixed position, can carry out three group's experiments simultaneously.
Specifically, the fixing frame 18 is provided with a screw rod 19 and a nut 20, the nut 20 is matched with the screw rod 19, and the nut 20 is used for fixing the first injector 2 and the second injector 3. Can be adjusted according to the specification of the used syringe, so that the device can adapt to syringes with different specifications.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. The device for carrying out chemical fixation under high pressure is characterized by comprising an autoclave, and a first injector, a second injector and a first pressure limiting valve which are arranged in the autoclave, wherein the first injector and the second injector are mutually communicated through the first pressure limiting valve, the first injector and the second injector are respectively provided with a first piston for changing the volume of the injector, the first piston moves under the pushing of a power system, the power system comprises a second piston and a pressure pump, the second piston moves in the autoclave and divides the autoclave into a first containing cavity and a second containing cavity, the first injector and the second injector are arranged in the second containing cavity, and the pressure pump is communicated with the first containing cavity.
2. The device of claim 1, wherein the first pressure limiting valve comprises an elastic hose and a plug, the two ends of the elastic hose are respectively connected with the first syringe and the second syringe, the plug is arranged in the elastic hose, and the diameter of the plug is larger than the inner diameter of the elastic hose.
3. The device of claim 2, wherein the flexible tube is a silicone tube and the plug bead is a stainless steel bead.
4. The device of claim 1, wherein the autoclave is connected to a second pressure-limiting valve and a pressure gauge, the pressure gauge is used for detecting the pressure in the autoclave, the water inlet end of the second pressure-limiting valve is communicated with the autoclave, and a first liquid inlet is arranged between the second pressure-limiting valve and the autoclave.
5. The device for chemical fixation under high pressure of claim 4, wherein the outlet end of the second pressure limiting valve is connected to a waste tank.
6. An apparatus for chemical anchoring at high pressure as defined in claim 5, wherein said waste cylinder is provided with a scale.
7. The device for chemical anchoring at high pressure of claim 1, further comprising a water reservoir, said pressurizing pump being connected to said water reservoir.
8. The apparatus of claim 1, wherein a fixture is disposed in the autoclave, and the first injector and the second injector are fixed to the fixture.
9. The device as claimed in claim 8, wherein the fixing frame is provided with a screw and a nut, the nut is engaged with the screw, and the nut is used for fixing the first syringe and the second syringe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810175173.5A CN108489780B (en) | 2018-03-02 | 2018-03-02 | Device for chemical fixation under high pressure |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810175173.5A CN108489780B (en) | 2018-03-02 | 2018-03-02 | Device for chemical fixation under high pressure |
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| Publication Number | Publication Date |
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| CN108489780A CN108489780A (en) | 2018-09-04 |
| CN108489780B true CN108489780B (en) | 2021-04-13 |
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| CN201810175173.5A Active CN108489780B (en) | 2018-03-02 | 2018-03-02 | Device for chemical fixation under high pressure |
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