CN108477443A - A kind of clearing composite beverage and its processing method with anti-inflammatory effect - Google Patents

Abstract

The invention relates to the technical field of comprehensive utilization of agricultural and sideline product waste, in particular to a throat-clearing compound beverage with anti-inflammatory effect, which is mainly made of the following raw materials in parts by mass: 3 to 7 parts of loquat leaves, 0.5 to 3 parts of loquat flowers 0.3-2 parts of honeysuckle and 0.2-2 parts of Panda Dahai, the loquat leaves, loquat flowers, honeysuckle and Panda Dahai are extracted with hot water and then concentrated to obtain the throat-clearing compound drink. A processing method thereof is also provided. The beverage made from the formula of the present invention has a unique aroma of flowers and plants. Many people tested the taste to be slightly sweet and moist, achieving the purpose of pleasant health preservation; the raw materials loquat leaves and loquat flowers are by-products of loquat production and processing, and the cost is low. Turning waste into treasure can extend the loquat industrial chain and expand the utilization width of loquat, which will bring good economic and social benefits.

Description

A throat-clearing compound beverage with anti-inflammatory effect and processing method thereof

technical field

The invention relates to the technical field of comprehensive utilization of agricultural and sideline product waste, in particular to a functional drink with the effect of clearing throat and throat and a processing method thereof, which is produced from loquat leaves and flowers as main raw materials.

Background technique

Loquat (Eriobotrya japonica Lindl), Rosaceae, is an evergreen small tree and is a subtropical specialty fruit tree. It is mainly distributed in East China, Central South and Southwest China. It has a history of more than 2,000 years of loquat planting, and the national loquat production has exceeded 3.8 tons. Loquat is a kind of fruit that is deeply loved by everyone. The whole body of loquat is full of treasures. The fruit, kernel, leaves and flowers of loquat all have the functions of moisturizing the lungs and relieving cough, reducing adverse reactions and relieving vomiting. It is used as a traditional Chinese medicine in traditional Chinese medicine. Loquat leaves have been included in the list of new food raw materials; loquat flowers have a history of eating among the people, and loquat flowers in some areas have also been listed as common foods. Loquat leaves and flowers are rich in active ingredients, such as saponins, triterpenoids, polyphenols, flavonoids, polysaccharides, etc., which have various physiological activities, such as enhancing the body's immunity, preventing cardiovascular system diseases, stabilizing liver function, Anti-tumor, anti-inflammatory, antibacterial, hypolipidemic, hypoglycemic and other effects.

Pharynx physiologically refers to the throat, including the passage of the pharynx, upper esophagus, larynx, and trachea leading to the stomach and lungs, and the upper front of the neck next to the cheek. The main functions of the pharynx are swallowing, breathing and pronunciation. In addition, the tonsils in the pharynx have functions such as phagocytosis of bacteria, production of immune antibodies, and production of lymphocytes, and are the "guards" of the respiratory tract. The main function of the larynx is that the larynx is the only way for normal breathing, and it is also an important pronunciation organ. The throat is the first barrier of the human body and plays an important role in the respiratory tract. However, due to the high pressure and social activities of modern people, as well as the influence of smoking, drinking, air pollution and colds, the throat is the first to be injured. Therefore, pharyngitis is now Especially the crowd of chronic pharyngitis increases.

In order to be able to protect our throats which are increasingly in a bad environment, the present invention achieves the purpose of clearing the throat through the beverage made of food raw materials. Both loquat leaves and flowers are rich in active ingredients. According to domestic and foreign literature reports, both loquat leaves and flowers have good anti-inflammatory effects, such as Ju Jianhua et al. Studies have shown that the ethyl acetate and n-butanol extracts of the ethanol extract of loquat leaves have a significant inhibitory effect on mouse ear swelling caused by xylene; Significantly inhibited mouse ear swelling caused by xylene and reduced the number of coughs in mice within 3 minutes; Deng Jingjing et al.'s research on the antitussive and anti-inflammatory activities of loquat flower showed that the low-dose water extract and high-dose alcohol extract group of loquat flower It has obvious effect of inhibiting mouse ear swelling.

Therefore, the present invention will use loquat leaves and flowers as main raw materials, and through scientific compatibility, supplemented with other foods with throat-clearing effect or raw materials with the same source of medicine and food, a compound drink with throat-clearing and throat-clearing effects will be obtained.

Contents of the invention

In order to achieve the purpose of clearing the throat and sharpening the throat, and as a kind of food and beverage, the present invention selects food or medicinal and edible homologous raw materials that are believed to have pharmacological activities such as clearing the throat, clearing the lungs, moistening the lungs, anti-inflammation and preventing colds. Various raw materials are proportioned according to different properties and flavors, strength of action, cost, etc.; in order to increase the content of active ingredients, the raw materials are extracted; in order to increase the effect per unit volume of the product and facilitate portability, the extract is concentrated.

In order to realize the purpose of the invention of the present invention, the inventor provides the following technical solutions:

A throat-clearing compound beverage with anti-inflammatory effect, mainly made of the following raw materials in parts by mass:

3 to 7 parts of loquat leaves, 0.5 to 3 parts of loquat flowers, 0.3 to 2 parts of honeysuckle and 0.2 to 2 parts of Panda Dahai. throat-clearing compound drink.

The herbal prescription of the present invention is on the basis of syndrome differentiation, according to the needs of the situation, utilizes the efficacy of herbs, stipulates necessary amounts, and compatibly organizes prescriptions. Loquat leaves return to the lungs and liver meridian, disperse wind-heat, calm the liver and improve eyesight, clear away heat and detoxify, have anti-oxidation effects, and can fight against free radicals increased by smoking. Loquat flower returns to the lung meridian, relieves wind and relieves cough. Honeysuckle and Panghaigui Gui Lung, Heart, and Stomach meridian are also good medicines for clearing away heat and detoxifying, anti-inflammatory, tonifying deficiency and curing wind, suitable for sore throat and tonsillitis caused by smoking. The application of compatible prescriptions between herbs plays an important role in improving efficacy and reducing side effects. The beverage made from the formula of the present invention has a unique fragrance of flowers and plants, and the taste is slightly sweet and moist in tests by many people, so as to achieve the purpose of pleasant health preservation.

As preferably, in the present invention, above-mentioned loquat leaf is used after drying, cutting into strips. It is convenient for hot water to extract active ingredients.

As a preference, in the present invention, the above-mentioned loquat flower is used after being pulverized. It is convenient for hot water to extract active ingredients.

Preferably, in the present invention, the above-mentioned honeysuckle is coarsely ground and used. It is convenient for hot water to extract active ingredients.

As preferably, in the present invention, the above-mentioned hot water extraction refers to the extraction of raw materials with a good ratio at 100°C in a slightly boiling state, the solid-liquid ratio is 1:5-20, the extraction time is 0.5-4h, and the number of extractions is 1-20. 5 times, after the extraction is completed, filter and combine the extracts.

Preferably, in the present invention, the above-mentioned concentration refers to direct concentration and vacuum concentration of the extract obtained by hot water extraction according to a concentration ratio of 2 to 6 times.

The present invention also provides a processing method of the above-mentioned throat-clearing compound drink with anti-inflammatory effect, comprising:

(1) Weighing and preprocessing raw materials

According to the mass ratio of 3 to 7 parts of loquat leaves, 0.5 to 3 parts of loquat flowers, 0.3 to 2 parts of honeysuckle and 0.2 to 2 parts of fat sea, weigh raw materials, dry and cut loquat leaves, coarsely powder loquat flowers, and coarsely powder honeysuckle ,

(2) Hot water extraction

The loquat leaves, loquat flowers, honeysuckle and Panda Dahai in step (1) are extracted with hot water, extracted under a slight boiling state at 100°C, the ratio of solid to liquid is 1:5-20, the extraction time is 0.5-4h, and the number of times of extraction is 1 ~5 times, after the extraction, filter and combine the extracts,

(3) concentrated

The extract obtained in step (2) is concentrated in a vacuum or directly, and the concentration ratio is 2 to 6 times.

(4) Filling and sterilization

The concentrated liquid obtained in step (3) is filled into a concentrated drinking liquid of 20-30 ml by a filling machine, and then sterilized by steam to obtain a finished beverage.

As a preferred version, in the present invention, steam sterilization treatment in the above-mentioned step (4) refers to treatment at a temperature of 120 degrees Celsius for 20 minutes.

The drinking method of the beverage of the present invention is, based on the amount of 20-30mL beverage in each bottle: directly orally or infusion, when infusion, take 5-10ml, dilute and drink, can add rock sugar or honey to taste, can be several times a day, and the symptoms are severe The dosage can be increased when the time comes, no more than 4 bottles per day. The dosage or frequency of taking can be reduced in the later stage of symptom relief, such as once a day, 10-20ml at a time, until the symptoms basically disappear.

Compared with prior art, the beneficial effect of the present invention is:

(1) The throat-clearing compound beverage of the present invention is suitable for people living in bustling cities, areas with high air pollution and dirty air, as well as people who smoke and drink for a long time, indirect smoking, cough due to lung heat, itching in the throat and the like;

(2) The throat-clearing compound drink of the present invention has a relatively high content of active ingredients, wherein the content of triterpene acids is 5.6-8.6 g/L, the content of polyphenols is 1.5-7.1 g/L, and the content of flavonoids is 4.5-15.5 g/L g/L and polysaccharide content are 0.6~4.7g/L;

(3) After the throat-clearing compound beverage product that the present invention obtains is tested by the surrounding crowd, the feedback effect shows that it has a more obvious effect on throat inflammation and cough (the surrounding crowd took more than 50 examples, and the symptoms showed as throat swelling and pain, throat inflammation, coughing, etc.) etc., obviously relieved after taking);

(4) The method provided by the present invention is simple in process, safe and environmentally friendly, low in cost, strong in operability, and easy for actual production and application;

(5) the raw material loquat leaf and loquat flower that the present invention adopts are by-products of loquat production and processing, with low cost, turning waste into wealth, prolonging the industrial chain of loquat, expanding the utilization width of loquat, will bring good economy and social benefits.

Instructions attached

Fig. 1 is the total triterpene acid standard curve of the throat-clearing compound beverage of the present invention.

Fig. 2 is the standard curve of total flavonoids and total phenols of the throat-clearing compound drink of the present invention.

Fig. 3 is the total sugar standard curve of the compound drink for clearing throat of the present invention.

Fig. 4 is a product picture of the throat clearing compound drink of the present invention.

Detailed ways

Below in conjunction with embodiment, the content of the present invention is described more specifically. It should be understood that the implementation of the present invention is not limited to the following examples, and any modifications and/or changes made to the present invention will fall within the protection scope of the present invention.

In the present invention, unless otherwise specified, all parts and percentages are weight units, and all equipment and raw materials can be purchased from the market or commonly used in this industry. Unless otherwise specified, the methods used in the examples are common techniques in the art.

Example 1

Dried loquat leaves are cut into strips, weighing 4 parts, loquat flowers are coarsely crushed, weighing 3 parts, honeysuckle is coarsely crushed, weighing 2 parts, Panda Dahai directly weighs 1 part, all raw materials are mixed, and added to the extraction tank. Add water according to the ratio of material to liquid 1:5, stir and mix evenly, heat to 100°C, and extract at 100°C for 0.5h, filter, add the same volume of water to the filter residue, extract again for 0.5h, filter, and combine the filtrates. The filtrate was concentrated under vacuum to about 2 times. The concentrated solution is filled into 30ml packs through a filling machine, steam sterilized (at a temperature of 120 degrees Celsius, for 20 minutes), and stored. The contents of triterpene acids, polyphenols, flavonoids and polysaccharides in the measured product were 6.1g/L, 1.5g/L, 4.5g/L, 0.6g/L respectively. And the filtrate is directly heated and concentrated, and also concentrated to about 2 times. The contents of triterpene acids, polyphenols, flavonoids and polysaccharides in the product measured by the same method are 4.8g/L, 1.4g/L, 4.3g/L, and 1.4g respectively. /L.

Example 2

The pretreatment method of the four kinds of raw materials is the same as in Example 1, and the proportioning ratio of the raw materials is as follows: 5 parts of loquat leaves, 2 parts of loquat flowers, 2 parts of honeysuckle, and 1 part of fat sea. All raw materials are mixed and added to the extraction tank. Add water according to the ratio of material to liquid 1:20, stir and mix evenly, heat to 100°C, keep warm for 3 hours, filter, extract the filter residue twice in the same way, filter, and combine the filtrates. The filtrate was concentrated in vacuo to about 3-fold. The concentrated solution is filled into 30ml packs through a filling machine, steam sterilized (at a temperature of 120 degrees Celsius, for 20 minutes), and stored. The contents of triterpene acids, polyphenols, flavonoids and polysaccharides in the measured product were 5.5g/L, 1.6g/L, 6.2g/L, 0.6g/L respectively.

Example 3

The pretreatment method of the four kinds of raw materials is the same as in Example 1, and the proportioning ratio of the raw materials is as follows: 6 parts of loquat leaves, 2 parts of loquat flowers, 1 part of honeysuckle, and 1 part of fat sea. All raw materials are mixed and added to the extraction tank. Add water according to the ratio of solid to liquid 1:20, stir and mix evenly, heat to 100°C, keep warm for 3h, filter, add the same volume of water, extract again for 3h, filter, and combine the filtrates. The filtrate was concentrated in vacuo to about 3-fold. The concentrated solution is filled into 30ml packs through a filling machine, steam sterilized (at a temperature of 120 degrees Celsius, for 20 minutes), and stored. The contents of triterpene acids, polyphenols, flavonoids and polysaccharides in the measured product were 8.7g/L, 8.1g/L, 15.5g/L, 4.7g/L respectively.

test part

Experimental Example 1 Effect of Qingyan compound drink on NO production of RAW264.7 cells induced by LPS

RAW264.7 cells in the logarithmic growth phase were inoculated in 96-well plates at 1×10 ⁶ cells/ml. After 24 hours, the culture medium was discarded, and given modeling or drug treatment respectively. The normal group, LPS model group (8 μg/ml), For each dose group of Qingyan Concentrated Drinking Liquid (8 μg/ml LPS+compounds of different concentrations, four-fold dilution from 1/2 to 1/8192, a total of 7 concentrations), 3 replicate wells were set up for each group. After culturing in the incubator for 24 hours, collect the cell supernatant of each well, operate according to the instructions of the NO kit, and read the absorbance value of each well under the condition of 540nm wavelength to calculate the NO content. The results are shown in Table 1.

Table 1 Effect of Compound Drink on LPS-Induced Inflammation RAW264.7 Cell Supernatant NO Content (μM) (n=3)

Note: Compared with normal control group, **P<0.01; compared with LPS model group, ^# P<0.05, ^## P<0.01, ^### P<0.001.

Compared with the LPS group, each concentration group of Qingyan Concentrated Drinking Liquid can reduce the NO content in the supernatant of RAW264.7 cells, and there is a significant difference between the 1/2 to 1/2048 concentration group compared with the LPS group, 1/2 The 128 to 1/2048 group showed obvious dose dependence, and the statistical analysis was carried out by T test. The P value was less than 0.05, 0.01 or 0.001, and the difference was statistically significant.

The assay method of test example 2 total triterpene acids, total phenols, total flavonoids and total sugars

Preparation of total triterpene acid standard song: Accurately weigh 20mg of oleanolic acid reference substance, put it in a 100mL volumetric flask, add absolute ethanol to dissolve, and dilute to 100mL (concentration 0.2g/L) as the reference substance solution. Precisely draw 0.2, 0.4, 0.6, 0.8, 1.0mL of the reference substance solution, place them separately in a stoppered test tube, evaporate the solvent, add 0.2mL of 5% vanillin-glacial acetic acid solution, 1.0mL of perchloric acid, and bathe in water at 60°C for 15min , cooled in an ice bath, added glacial acetic acid to make up to volume, shaken well, and measured the absorbance at a wavelength of 548nm. After regression analysis, the standard curve and equation are shown in Figure 1. For sample determination, 1ml of the extract was used instead of the standard solution, and the concentration of total triterpene acids in the compound drink was calculated through the standard curve equation and the dilution factor.

Production of total phenol standard song: take 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8, 2.0ml of gallic acid standard solution with a concentration of 0.1mg/ml in a 25ml volumetric flask, add 2.5ml of Folin Ciocalteu chromogen, shake well; then add 5ml 5% sodium carbonate solution, add water to make up the volume, place it in the dark at 25°C for 1 hour, measure the absorbance value at 750nm, take the concentration of the standard as the abscissa, and the absorbance value as the ordinate The coordinates are used as a standard curve, and the standard curve and equation are shown in Figure 2. For sample determination, 1ml of the extract was used instead of the standard solution, and the concentration of total phenols in the compound drink was calculated by the standard curve equation and the dilution factor.

Production of total flavonoid standard song: accurately weigh an appropriate amount of anhydrous rutin reference substance, add ethanol to make a 0.2mg/ml reference substance solution. Precisely draw 1, 2, 3, 4, 5, and 6ml of the solution into 25ml volumetric flasks, add ethanol to 6ml, add 1ml of 5% 1ml/L sodium nitrite solution, shake well, and place for 6min; add 10% nitric acid Aluminum solution 1ml, shake well, stand for 6min; add 10ml of 4% sodium hydroxide solution, shake well; add ethanol to the mark, shake well, stand for 15min. Measure the absorbance at 509nm. Make a standard curve, see Figure 2 for the standard curve and equation. For sample determination, 1ml of extract was used instead of standard solution, and the concentration of total flavonoids in the compound drink was calculated through the standard curve equation and dilution factor.

Production of total sugar standard song: Accurately weigh 5mg of glucose, dissolve it in a small amount of distilled water, transfer it to a 50ml volumetric flask, and use distilled water to make up a 100μg/ml glucose solution, absorb 0, 0.2, 0.4, 0.6, 0.8, Put 1.0ml in a test tube and add water to make up to 1ml, add 1ml of 5% phenol, quickly add 5ml of concentrated sulfuric acid, after cooling, adjust to zero with a zero tube, and measure the absorbance at 490nm. Make a standard curve, see Figure 3 for the standard curve and equation. For sample determination, 1ml of the extract was used instead of the standard solution, and the concentration of total sugar in the compound drink was calculated through the standard curve equation and the dilution factor.

The trial effect of the compound drink on the people around the application example

More than 50 cases were tested by employees of the unit and their families, as well as their relatives and friends distributed to them. Their main symptoms were sore throat, cough, throat inflammation, and hoarseness caused by colds, smoking, and excessive use of the throat. etc. After taking 10-30ml of the compound drink, the symptoms were alleviated, and after taking 5-6 bottles, the symptoms were obviously alleviated or disappeared. After that, if there are still some mild symptoms, reduce the dosage or frequency of taking, such as once a day, until the symptoms disappear. Here are a few typical trial examples to further illustrate the trial effect:

Application example 1

An employee of our unit, male, 57 years old, with symptoms of dry throat and cough, took 15ml when the symptoms improved, and took a total of 60ml, and the pharynx felt better after taking it.

Application example 2

An employee of our unit, male, 30 years old, the symptom is dry throat caused by smoking, the dosage is 15ml when the symptoms are improved, and a total of 60ml is taken, and the reaction effect is not bad.

Application example 3

The family member of this unit, female, 4 years old, the symptom is hoarseness, the dosage when the symptom improves is 6ml, takes 150ml altogether, and the reaction effect is not bad.

Application example 4

An employee of our unit, male, 50 years old, the symptom is chronic pharyngitis caused by smoking, the dosage is 30ml when the symptoms are improved, and a total of 300ml is taken, and the reaction effect is not bad.

Application example 5

An employee of our unit, female, 40 years old, the symptom is a cough caused by a cold, the dosage is 10ml when the symptoms are improved, and a total of 100ml is taken, and the reaction effect is relatively obvious.

Application example 6

The family member of our unit, female, 25 years old, the symptom is hoarseness caused by excessive use of the voice, the dosage is 15ml when the symptoms are improved, and a total of 150ml is taken, and the response effect is very good.

Application example 7

The family member of this unit, female, 65 years old, the symptom is that the cough does not get better. When the symptoms improve, the dosage is 30ml, and a total of 100ml is taken, and the response effect is very good.

Application example 8

An employee of our unit, male, 50 years old, the symptom is sore throat, the dosage is 20ml when the symptoms are improved, and a total of 60ml is taken, and the response effect is very good.

Claims (7)

1. a throat-clearing compound beverage with anti-inflammatory effect, is characterized in that being mainly made from the raw material of following mass parts: 3 to 7 parts of loquat leaves, 0.5 to 3 parts of loquat flowers, 0.3 to 2 parts of honeysuckle and 0.2 to 2 parts of Panda Dahai. throat-clearing compound drink. 2. A kind of throat clearing compound drink with anti-inflammatory effect according to claim 1, is characterized in that, described loquat leaf is dried, uses after cutting into strips; use. 3. A throat-clearing compound beverage with anti-inflammatory effect according to claim 1, characterized in that, the hot water extraction refers to the extraction of raw materials with a good ratio at 100°C in a slightly boiling state, and the solid-liquid ratio The ratio is 1:5~20, the extraction time is 0.5~4h, and the number of extractions is 1~5 times. After the extraction is completed, filter and combine the extracts. 4. A throat-clearing compound beverage with anti-inflammatory effect according to claim 1, characterized in that, said concentration refers to that the extract obtained by hot water extraction is directly concentrated or vacuum-concentrated according to the concentration ratio of 2 to 6 times. concentrate. 5. A kind of throat-clearing compound beverage with anti-inflammatory effect according to claim 1, characterized in that, the drinking method of the throat-clearing compound beverage is, in the amount of 20-30mL beverage per bottle: directly orally Or take it after brewing. When taking it, take 5-10ml, dilute and drink, or add rock sugar or honey to taste. It can be several times a day. When the symptoms are severe, the dosage can be increased, not more than 4 bottles a day; the dosage can be reduced when the symptoms are relieved in the later stage. Or the frequency, such as once a day, 10-20ml each time, until the symptoms basically disappear. 6. The processing method of a throat-clearing compound beverage with anti-inflammatory effect according to any one of claims 1-5, characterized in that, the processing method comprises: (1) Weighing and preprocessing raw materials According to the mass ratio of 3 to 7 parts of loquat leaves, 0.5 to 3 parts of loquat flowers, 0.3 to 2 parts of honeysuckle and 0.2 to 2 parts of fat sea, weigh raw materials, dry and cut loquat leaves, coarsely powder loquat flowers, and coarsely powder honeysuckle , (2) Hot water extraction The loquat leaves, loquat flowers, honeysuckle and Panda Dahai in step (1) are extracted with hot water, extracted under a slight boiling state at 100°C, the ratio of solid to liquid is 1:5-20, the extraction time is 0.5-4h, and the number of times of extraction is 1 ~5 times, after the extraction, filter and combine the extracts, (3) concentrated The extract obtained in step (2) is concentrated in a vacuum or directly, and the concentration ratio is 2 to 6 times. (4) Filling and sterilization The concentrated liquid obtained in the step (3) is filled into 20-30 ml concentrated drinking liquid through a filling machine, and then steam sterilized to obtain a finished beverage. 7. The processing method of a throat-clearing compound beverage with anti-inflammatory effect according to claim 6, characterized in that, the steam sterilization treatment described in the step (4) refers to processing at a temperature of 120 degrees Celsius 15-30 minutes.