CN108465053A - A kind of preparation method of antineoplastic component in mao of arrowhead - Google Patents
A kind of preparation method of antineoplastic component in mao of arrowhead Download PDFInfo
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- CN108465053A CN108465053A CN201810221626.3A CN201810221626A CN108465053A CN 108465053 A CN108465053 A CN 108465053A CN 201810221626 A CN201810221626 A CN 201810221626A CN 108465053 A CN108465053 A CN 108465053A
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- arrowhead
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- mao
- ethyl acetate
- medicinal extract
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses the preparation methods of the antineoplastic component in a kind of mao of arrowhead, and steps are as follows：The drying pseudobulb of hair arrowhead is extracted 24 times with 85 95% alcohol reflux of volume fraction, 3 4h, merges extracting solution every time, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract to use petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol after appropriate water dissolution successively, it is concentrated under reduced pressure；It takes ethyl acetate portion to be detached with silica gel column chromatography, uses volume ratio 90 successively:1、70:1、55:1、30:1、15:1、1:1 dichloromethane methanol elution, collects 15:1 elution fraction, concentrates to obtain the final product.Preparation method provided by the invention is easy to operate, can quickly obtain the antineoplastic component in mao arrowhead, can be prepared applied to drug as the active component of hair arrowhead.
Description
Technical field
Present invention relates particularly to the preparation methods of the antineoplastic component in a kind of mao of arrowhead.
Background technology
Hair arrowhead is orchid Cremastra appendiculataCremastra appendiculata(D. Don) Makino's is dry false
Bulb is one of the source of Chinese medicine edible tulip.Its property is sweet, micro-pungent, cool, Return liver, the spleen channel, has clearing heat and detoxicating, reducing phlegm and resolving masses
Effect is mainly used for carbuncle swells serious case of furuncle, scrofula subcutaneous nodule, wei lumps in the abdomen lump in the abdomen, snakebite and bugbite, makes with Chinese herbal medicine for preventing compatibility on tcm clinical practice
With.Hair arrowhead contains there are many chemical composition, and main includes luxuriant and rich with fragrance class, bibenzyl, flavanone, alkaloids, terpene etc..
Invention content
The purpose of the present invention is to provide the preparation methods of the antineoplastic component in a kind of mao of arrowhead.
The present invention is realized by following technical solution：
The preparation method of antineoplastic component in a kind of mao of arrowhead, includes the following steps：By the drying pseudobulb body of hair arrowhead
Fraction 85-95% alcohol refluxs extract 2-4 times, each 3-4h, merge extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；It will leaching
Cream is concentrated under reduced pressure with using petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol after appropriate water dissolution successively；Take ethyl acetate
Part is detached with silica gel column chromatography, uses volume ratio 90 successively:1、70:1、55:1、30:1、15:1、1:1 methylene chloride-methanol
Elution collects 15:1 elution fraction, concentrates to obtain the final product.
Preferably, in the preparation method, the drying pseudobulb of hair arrowhead 90% alcohol reflux of volume fraction is extracted
3 times.
Preferably, in the preparation method, each refluxing extraction 3.5h.
Preferably, in the preparation method, 1kg maos of arrowheads carry out refluxing extraction using 4-6L solvents.
The technology of the present invention effect：
Preparation method provided by the invention is easy to operate, can quickly obtain the antineoplastic component in mao arrowhead, hair can be used as kind
The active component of mushroom is prepared applied to drug.
Specific implementation mode
The essentiality content of the present invention is specifically introduced with reference to embodiment.
Embodiment 1
The preparation method of antineoplastic component in a kind of mao of arrowhead, includes the following steps：By the drying pseudobulb body of hair arrowhead
90% alcohol reflux of fraction extracts 3 times, each 3.5h, merges extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract with
Petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol are used successively after appropriate water dissolution, are concentrated under reduced pressure；Take ethyl acetate portion
It is detached with silica gel column chromatography, uses volume ratio 90 successively:1、70:1、55:1、30:1、15:1、1:1 methylene chloride-methanol elution,
Collect 15:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg maos of arrowheads carry out circumfluence distillation using 5L solvents.
Embodiment 2
The preparation method of antineoplastic component in a kind of mao of arrowhead, includes the following steps：By the drying pseudobulb body of hair arrowhead
85% alcohol reflux of fraction extracts 2 times, each 3h, merges extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract with suitable
Petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol are used successively after measuring water dissolution, are concentrated under reduced pressure；Ethyl acetate portion is taken to use
Silica gel column chromatography detaches, and uses volume ratio 90 successively:1、70:1、55:1、30:1、15:1、1:1 methylene chloride-methanol elution, is received
Collection 15:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg maos of arrowheads carry out circumfluence distillation using 4L solvents.
Embodiment 3
The preparation method of antineoplastic component in a kind of mao of arrowhead, includes the following steps：By the drying pseudobulb body of hair arrowhead
95% alcohol reflux of fraction extracts 4 times, each 4h, merges extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract with suitable
Petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol are used successively after measuring water dissolution, are concentrated under reduced pressure；Ethyl acetate portion is taken to use
Silica gel column chromatography detaches, and uses volume ratio 90 successively:1、70:1、55:1、30:1、15:1、1:1 methylene chloride-methanol elution, is received
Collection 15:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg maos of arrowheads carry out circumfluence distillation using 6L solvents.
Preparation method provided by the invention is easy to operate, can quickly obtain the antineoplastic component in mao arrowhead, can conduct
The active component of hair arrowhead is prepared applied to drug.
Claims (4)
1. the preparation method of the antineoplastic component in a kind of mao of arrowhead, which is characterized in that include the following steps：By the dry of hair arrowhead
Dry pseudobulb is extracted 2-4 times, each 3-4h with volume fraction 85-95% alcohol refluxs, merges extracting solution, and extracting solution is concentrated under reduced pressure
Obtain medicinal extract；By medicinal extract to use petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, decompression dense after appropriate water dissolution successively
Contracting；It takes ethyl acetate portion to be detached with silica gel column chromatography, uses volume ratio 90 successively:1、70:1、55:1、30:1、15:1、1:1
Methylene chloride-methanol elutes, and collects 15:1 elution fraction, concentrates to obtain the final product.
2. preparation method according to claim 1, it is characterised in that：By the drying pseudobulb volume fraction of hair arrowhead
90% alcohol reflux extracts 3 times.
3. preparation method according to claim 2, it is characterised in that：Each refluxing extraction 3.5h.
4. preparation method according to claim 1, it is characterised in that：1kg maos of arrowheads carry out reflux using 4-6L solvents and carry
It takes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810221626.3A CN108465053A (en) | 2018-03-17 | 2018-03-17 | A kind of preparation method of antineoplastic component in mao of arrowhead |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810221626.3A CN108465053A (en) | 2018-03-17 | 2018-03-17 | A kind of preparation method of antineoplastic component in mao of arrowhead |
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| Publication Number | Publication Date |
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| CN108465053A true CN108465053A (en) | 2018-08-31 |
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| CN201810221626.3A Withdrawn CN108465053A (en) | 2018-03-17 | 2018-03-17 | A kind of preparation method of antineoplastic component in mao of arrowhead |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114163314A (en) * | 2021-12-23 | 2022-03-11 | 西安交通大学 | Extraction method of Blestiarene B in abacus chinensis and anti-tumor application thereof |
-
2018
- 2018-03-17 CN CN201810221626.3A patent/CN108465053A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114163314A (en) * | 2021-12-23 | 2022-03-11 | 西安交通大学 | Extraction method of Blestiarene B in abacus chinensis and anti-tumor application thereof |
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Application publication date: 20180831 |
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