CN108464396A - The animal feed additive from timber inhibited for quorum sensing - Google Patents
The animal feed additive from timber inhibited for quorum sensing Download PDFInfo
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- CN108464396A CN108464396A CN201710099375.1A CN201710099375A CN108464396A CN 108464396 A CN108464396 A CN 108464396A CN 201710099375 A CN201710099375 A CN 201710099375A CN 108464396 A CN108464396 A CN 108464396A
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- quorum sensing
- wood particle
- tree
- sensing inhibitor
- grass
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Abstract
The present invention relates at least one quorum sensing inhibitor for including the wood particle from tree and the wood particle from lignifying grass, it adjusts body temperature as feed addictive for the antibiotic-free infection prevention disease in farm-animals and pet and under thermal stress conditions, the tree is preferably selected from Pinaceae, Cupressaceae family and is selected from white poplar, and the lignifying grass is preferably selected from Bambusoideae families.
Description
The present invention relates to quorum sensing inhibitor.
In farm-animals production, a large amount of antibiotic have been used as growth that additive is promoted to use and for thin for causing a disease
The therapeutic reason of bacterium.In farm-animals and pet the two, the appearance of antibiotic resistance causes to give up in many countries anti-
It gives birth to element growth promoter and limits using therapeutic antibiotics.So-called " the de- pharmaceutical procedures（demedication
process）" actually in most of European countries, or even in such as Southeast Asia and North America and the generation of Hispanic region.Future
Strategy should be it is no outburst harmful bacteria disease risk in the case of avoid it is wide in range in farm-animals and pet
Abuse of antibiotics.Quorum sensing inhibits to be the pathogenic a kind of mode for reducing bacterium.Quorum sensing is the cell that bacterium uses
Communication between cell, to control the expression of certain genes (including toxic gene) by generating signaling molecule.Interference disease
The quorum sensing mechanism of substance is that the attraction of farm-animals and pet illness outbreak is controlled without using antibiotic
The potential mode of people.
The present invention provide with quorum sensing inhibitory activity product be used as feed addictive, for farm-animals with dote on
Antibiotic-free infection prevention disease in object.The quorum sensing inhibitor of the present invention includes wood particle from tree and from wood
At least one of the wood particle of elementization grass.According to invention feature, tree is selected from Pinaceae, Cupressaceae family and is selected from white poplar
Tree, lignifying grass are selected from Bambusoideae families.
Another feature according to the present invention, quorum sensing inhibitor also include glucosidase procyanidins.In a kind of embodiment party
In case, glucosidase procyanidins include 4-8 catechin unit.In another embodiment, glucosidase procyanidins include to be more than 8
Catechin unit.People carries out through the invention experiment it has been shown that wood particle with 4-8 and/or be more than 8 catechins
The effect for significantly improving the present invention is applied in combination in the glucosidase procyanidins of unit.
Another feature according to the present invention, wood particle exist in the form of fibrillation, and mean will be wooden for fibrillation
Material particle machinery size reduction to it is single or aggregation fibrillar structure.In one embodiment of the present invention, quorum sensing presses down
Preparation includes the combination of the wood particle and glucosidase procyanidins of fibrillation.
Another feature according to the present invention, feed addictive include at least grain size of 90 weight % in 50-500 μ ms
Fibrillation wood particle.
Another feature according to the present invention, other preferred compositions may be, for example, to come from Pinaceae from one or more
The mixture that the wood particle of the tree of family is optionally combined with the wood particle from white poplar, cypress and/or bamboo.In a kind of reality
It applies in scheme, any mixture of wood particle also may include glucosidase procyanidins.
The invention further relates to the feedstuff for farm-animals and pet, the feedstuff includes to be based on the feed
The quorum sensing inhibitor as defined above of the 0.05%-10% dose weights of the total weight of raw material, and it is at least one selected from
Under feed ingredient：Protein material, carbohydrate substance, crude fibre source, green feed preserve (ensilage), fat,
Vitamin, minerals and trace element.
The invention further relates to the pre-compositions for being used to prepare the above feedstuff for farm-animals and pet.The premix
Object includes the quorum sensing inhibitor of the present invention and at least one feed ingredient selected from the following：Protein material, carbon aquation
Close object substance, crude fibre source, green feed preservation (ensilage), fat, vitamin, minerals and trace element.
The invention further relates to quorum sensing inhibitor as defined above for especially adjusting farm under thermal stress conditions
The purposes of the body temperature of animal and pet.
In addition, the present invention relates at least one of the wood particle from tree and wood particle from lignifying grass works
Purposes for feed addictive for the antibiotic-free infection prevention disease in farm-animals and pet, the tree are preferably selected from
Pinaceae, Cupressaceae family and it is selected from white poplar, the lignifying grass is preferably selected from Bambusoideae families.
In addition, the present invention relates at least one of the wood particle from tree and wood particle from lignifying grass works
Purposes for feed addictive for adjusting farm-animals and the body temperature of pet especially under thermal stress conditions, the tree are preferred
Selected from Pinaceae, Cupressaceae family and it is selected from white poplar, the lignifying grass is preferably selected from Bambusoideae families.
Unexpectedly, the present invention can significantly inhibit the quorum sensing communication of pathogenic bacteria in farm-animals and pet, described
Bacterium is as Escherichia coli, salmonella（Salmonella）, staphylococcus (Staphylococci), campylobacter
(Campylobacter), streptococcus (Streptococci) etc..Therefore, feed addictive of the invention in farm-animals and is doted on
Excellent control is presented in the pathogenic factor (as enterotoxin, film forming etc.) of directed toward bacteria in object, and avoids disease in many cases
The outburst of disease, without using therapeutic antibiotics.Effect height is concerned, cannot especially influenced by standard antibiotic
Antibiotic-resistant bacterium, such as in ESBL bacteriums.On the other hand, highly desirable to reduce bacterium without any anti-microbial effect
The product of the outburst risk of caused disease because the anti-microbial effect of product not only breaks up pathogenic bacteria, or even adjust or
Intestinal flora is destroyed, the intestinal flora is by generating immune factor, vitamin and valuable metabolin (as volatile fat
Acid, bacillin etc.) it is important for organism.In short, it must be noted that the present invention does not influence the survivability of bacterium, but
Growth phase inhibits the formation of the harmful bacteria metabolin caused via the quorum sensing communication between bacterium.This is moved on farm
It is preferable to use entirely different with the effect of the antibiotic for the bacteriosis that contends with or antimicrobial material in object and pet.
Be described in detail the present invention referring now to embodiment, embodiment be intended to it is only illustrative, and not with any side
Formula limits the scope of the invention.
Embodiment 1
In vitro in test system, c-type bacillus aerogenes capsulatus is used（clostridium perfringen）β-
The effect of the Production of Toxin bacterial strain test present invention.By bacillus aerogenes capsulatus in 0.1% glucose-tryptone-salt training
It supports and is cultivated in base, be added without test substances (control) or the test substances of various concentration are added.Test substances are comprising by coming from
The quorum sensing inhibitor of the present invention of the product of the fibrillation of the mixture composition of the timber of dragon spruce and pine tree.Commodity in use is exempted from
The production of epidemic disease-experiment reagent box evaluation β toxin.As a result it presents in the following table 1.
Table 1
| The dosage of test substances | 0 (control) | 0.0001% | 0.001% | 0.01% | 0.1% | 1% |
| The production of β toxin | +++ | +++ | ++ | + | - | - |
+++ the high production of instruction；++ indicate medium production；The low production of+instruction；Instruction does not produce β toxin.
Embodiment 2
It is 360 by number in the commodity piggy farm with apparent enterorrhagia coli-infection problem in studying at the scene
Piggy（a number of 360 piglets）It is divided into 6 groups after wean.All animals receive the commodity for small weaning pig
Standard diet (table 2)." zero control group " receives the feed that any therapeutic agent or additive is not added.For " antibiotic group "
(positive control group), by neomycin with 10 mg neomycinsulphates/kg body weight/days " at top（on-top）" therapeutic dose add
Enter into feed.Four test groups receive the wean diet for the test substances that there is " at top " various concentration is added.Tester
Matter is the quorum sensing inhibitor of the present invention comprising the product being made of the timber from dragon spruce and pine tree.
Table 2
| Wheat | 27.40% |
| Barley | 25.00% |
| Corn | 20.00% |
| Soybean-cake flour HP | 16.30% |
| Wheat bran | 5.30% |
| Soya-bean oil | 1.00% |
| Mono phosphoric acid ester calcium | 0.70% |
| Calcium carbonate | 0.65% |
| Salt | 0.35% |
| Fish oil | 0.10% |
| Pre-composition, acid | 3.20% |
During 10 days testing time sections, for all groups, Incidence of Diarrhea is reported.
In testing, even about the body temperature of animal, the effect of the feed addictive of the present invention is evaluated.As a result in following table
It is presented in 3.
Table 3
By table 3 as it can be seen that in the animal of acceptance test substance (that is, quorum sensing inhibitor of the present invention), with metering-relying party
Formula, Incidence of Diarrhea significantly reduce.
In addition, test substances can significantly reduce raised body temperature.Completely it was unexpected that with the another of non-infection animal
In one test system, it can be seen that feed addictive of the invention can slight decrease normal body temperature to specific degree, especially
It is the raised body temperature under environment thermal stress conditions.Effect height is concerned, because body temperature is to body metabolism and energy expenditure
With directly affecting.Therefore, the energy requirement of growth and production can be significantly reduced by slightly reducing mean body temperature.In addition, in pig, family
Fowl, even in ruminant under common thermal stress conditions, in farm-animals, adjusting the effect of body temperature, also height is closed
Note.It was furthermore observed that effect in pet (such as dog, kind especially sensitive to hot conditions (in summer, become mildewed kind))
In be concerned.
Embodiment 3
In studying at the scene, in commodity breed farm, by the sow for the health for being in nursing period second week that quantity is 50
It is divided into five groups.All animals receive the commercial standards diet (table 4) for sow.Control group receives being somebody's turn to do without any additive
Diet.Four test groups receive the wean diet of the test substances for the various concentration that " at top " is added.Test substances be comprising
By the quorum sensing inhibitor of the invention for the product that the mixture from dragon spruce and the timber of pine tree forms.The 7th of experiment the
Its (third week of nursing period), body temperature (rectal methods) is measured in all sows.Table 5 shows each group of mean body temperature.
Table 4
| Barley | 40% |
| Corn | 11% |
| Wheat | 10% |
| Coleseed and sunflower disc powder | 11% |
| Small wheat cake powder | 15% |
| Wheat bran | 5% |
| Beet pulp | 5% |
| Mineral premix | 3% |
Table 5
Embodiment 4
In the feeding trial of 250 healthy piggys, about the influence to body temperature, the test present invention.After wean when 12 week old
Start to test, persistently carry out 7 days.All piggys receive standard piggy diet (referring to embodiment 1, table 2).Control group receiving does not have
The diet of any addition.Four test groups receive the diet of the test substances for the various concentration that there is " at top " to be added.
Test substances are the quorum sensing inhibitor of the invention comprising the product being made of the timber from dragon spruce and pine tree.It is trying
The 7th day tested measures the body temperature (rectal methods) of all piggys.Table 6 shows each group of mean body temperature.
Table 6
Embodiment 5
In 250 male broiler chicken（broiler）Feeding trial in, about under thermal stress conditions to the influence of body temperature, test
The present invention.Started to test at 21 day age.All male broiler chicken receive standard male broiler chicken diet (table 7).Control group receiving does not have
There is the diet of any addition.Four test groups receive the meals of the test substances for the various concentration that there is " at top " to be added
Food.Test substances are comprising having the low of 4-8 catechin unit by timber and 0.1 weight % amounts from dragon spruce and pine tree
Poly- procyanidine combines and the quorum sensing inhibitor of the present invention of the product of composition.According to guide, male broiler chicken is maintained at
Standardized room temperature.At the 28th day, the room temperature in fence is increased to 36 DEG C, up to 3 hours.At the end of this thermal stress period,
The body temperature of all animals is measured by infrared thermography method.Table 8 shows each group of mean body temperature.
Table 7
| Corn | 50% |
| Wheat | 10% |
| Beancake powder | 32% |
| Mineral premix | 3% |
| Fat | 5% |
Table 8
Embodiment 6
It is 360 by quantity in the commodity piggy farm with apparent enterorrhagia coli-infection problem in studying at the scene
Piggy be divided into 6 groups after wean.All animals receive the commercial standards diet for small weaning pig (referring to embodiment 1, table
2).Zero control group receives the feed that any therapeutic agent or additive is not added.For neomycin control group, by neomycin with
The therapeutic dose of 10 mg neomycinsulphates/kg body weight/days is added in feed.Four test groups receive that various concentration is added
Test substances wean diet.In this example, test substances are timber and 0.3 weight % amounts from dragon spruce and pine tree
Mixture with the glucosidase procyanidins combination more than 8 catechin units.During 10 days testing time sections, for institute
There is group, reports Incidence of Diarrhea.As a result it is presented in following table 9.
Table 9
By table 9 as it can be seen that in receiving the animal of feed addictive of the present invention, Incidence of Diarrhea significantly reduces.Compared in table 3
The experimental condition for the product that the use of display is not combined with glucosidase procyanidins, effect are more notable.
Claims (12)
1. it is a kind of be characterized in that it includes the wood particle from tree and the wood particle from lignifying grass it is at least one
Quorum sensing inhibitor（quorum sensing inhibitor）, it is used in farm-animals and pet as feed addictive
Middle antibiotic-free infection prevention disease, the tree are preferably selected from Pinaceae, Cupressaceae family and are selected from white poplar, the lignifying grass
It is preferably selected from Bambusoideae families.
2. the quorum sensing inhibitor of claim 1, it is characterised in that it includes the timber selected from the tree from Pinaceae family
Grain, the wood particle of the tree from Cupressaceae family and come from the wood particle of the wood particle from white poplar
Any mixture of the wood particle of the lignifying grass of Bambusoideae families.
3. the quorum sensing inhibitor of any one of claims 1 or 2 also includes glucosidase procyanidins.
4. the quorum sensing inhibitor of claim 3, wherein the glucosidase procyanidins include 4-8 catechin unit.
5. the quorum sensing inhibitor of claim 3, wherein the glucosidase procyanidins include to be more than 8 catechin units.
6. the quorum sensing inhibitor of any one of claim 1-5, which is characterized in that the wood particle is the wood of fibrillation
Material particle.
7. the quorum sensing inhibitor of any one of claim 1-6, it is characterised in that the grain size it includes at least 90 weight % is
The wood particle of 50-500 microns of fibrillation.
8. the quorum sensing inhibitor of any one of claim 1-7, dynamic for adjusting farm especially under thermal stress conditions
The body temperature of object and pet.
9. the feedstuff for farm-animals and pet, it is characterised in that it contains the total weight based on the feedstuff
The quorum sensing inhibitor of any one of the claim 1-8 of 0.05%-10% dose weights, and it is at least one selected from the following
Feed ingredient：Protein material, carbohydrate substance, crude fibre source, green feed preserve (ensilage), fat, dimension life
Element, minerals and trace element.
10. a kind of pre-composition for the feedstuff being used to prepare claim 9, the pre-composition includes to appoint in claim 1-8
One quorum sensing inhibitor and at least one feed ingredient selected from the following：Protein material, carbohydrate substance,
Crude fibre source, green feed preserve (ensilage), fat, vitamin, minerals and trace element.
11. at least one of the wood particle from tree and wood particle from lignifying grass are as with quorum sensing suppression
Purposes of the active feed addictive for the antibiotic-free infection prevention disease in farm-animals and pet is made, the tree is excellent
Choosing is selected from Pinaceae, Cupressaceae family and is selected from white poplar, and the lignifying grass is preferably selected from Bambusoideae families.
12. at least one of the wood particle from tree and wood particle from lignifying grass are as with quorum sensing suppression
Make purposes of the active feed addictive for adjusting farm-animals and the body temperature of pet especially under thermal stress conditions, the tree
It is preferably selected from Pinaceae, Cupressaceae family and is selected from white poplar, the lignifying grass is preferably selected from Bambusoideae families.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710099375.1A CN108464396A (en) | 2017-02-23 | 2017-02-23 | The animal feed additive from timber inhibited for quorum sensing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710099375.1A CN108464396A (en) | 2017-02-23 | 2017-02-23 | The animal feed additive from timber inhibited for quorum sensing |
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| Publication Number | Publication Date |
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| CN108464396A true CN108464396A (en) | 2018-08-31 |
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| CN201710099375.1A Pending CN108464396A (en) | 2017-02-23 | 2017-02-23 | The animal feed additive from timber inhibited for quorum sensing |
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Citations (7)
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| CN1552234A (en) * | 2003-05-27 | 2004-12-08 | 杭州利欣生物科技有限公司 | Feed additive for improving animal disease-resistant ability |
| CN1669460A (en) * | 2004-03-16 | 2005-09-21 | 上海朝翔生物技术有限公司 | Front-anthocyanidin feed addictive |
| WO2008058698A1 (en) * | 2006-11-13 | 2008-05-22 | Grabherr, Helmut | Roughage |
| US20100129302A1 (en) * | 2008-11-25 | 2010-05-27 | Oy Granula Ab Ltd | Antimicrobial composition with low cytotoxicity |
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