CN108459068A - Gel preparation automation equipment and application method - Google Patents
Gel preparation automation equipment and application method Download PDFInfo
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- CN108459068A CN108459068A CN201810615436.XA CN201810615436A CN108459068A CN 108459068 A CN108459068 A CN 108459068A CN 201810615436 A CN201810615436 A CN 201810615436A CN 108459068 A CN108459068 A CN 108459068A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 27
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 14
- 230000008676 import Effects 0.000 claims abstract description 4
- 239000000975 dye Substances 0.000 claims description 35
- 239000007788 liquid Substances 0.000 claims description 34
- 238000001816 cooling Methods 0.000 claims description 19
- 239000004065 semiconductor Substances 0.000 claims description 14
- 238000003860 storage Methods 0.000 claims description 14
- 239000000126 substance Substances 0.000 claims description 14
- 239000003292 glue Substances 0.000 claims description 12
- 230000004927 fusion Effects 0.000 claims description 11
- 230000033001 locomotion Effects 0.000 claims description 11
- 230000002572 peristaltic effect Effects 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 10
- 238000005192 partition Methods 0.000 claims description 8
- 238000001179 sorption measurement Methods 0.000 claims description 8
- 230000009471 action Effects 0.000 claims description 7
- 238000010276 construction Methods 0.000 claims description 7
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 7
- 239000000701 coagulant Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 230000009514 concussion Effects 0.000 claims description 4
- 238000009833 condensation Methods 0.000 claims description 4
- 230000005494 condensation Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 230000005611 electricity Effects 0.000 claims description 3
- 230000007246 mechanism Effects 0.000 claims description 3
- 230000002195 synergetic effect Effects 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims 1
- 231100000331 toxic Toxicity 0.000 claims 1
- 230000002588 toxic effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 description 11
- 239000000463 material Substances 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 238000010586 diagram Methods 0.000 description 6
- 239000007789 gas Substances 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 230000001473 noxious effect Effects 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000000711 cancerogenic effect Effects 0.000 description 2
- 231100000315 carcinogenic Toxicity 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000980 acid dye Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical group [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002341 toxic gas Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K1/00—Details of thermometers not specially adapted for particular types of thermometer
- G01K1/14—Supports; Fastening devices; Arrangements for mounting thermometers in particular locations
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Cosmetics (AREA)
- Medicinal Preparation (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides gel preparation automation equipment and application methods, belong to gel production field, including gel bottle, the upper end of the gel bottle is equipped with the inlet tube and dye applicator that the import with the gel bottle is correspondingly arranged, the gel bottle can move up and down, the gel bottle can be rotated along horizontal axis that is vertical with its own axis and intersecting, and the lower end of the gel bottle is equipped with heating plate and gel mold.The invention avoids people to contact with reagent, promotes security system, while realizing automation control, saves manpower, reduces labor intensity, has saved the preparation time of gel, improved work efficiency, easy to use and reliable.
Description
Technical field
The invention belongs to gel production fields, are related to gel preparation automation equipment and application method.
Background technology
Agarose nucleic acid gel electrophoresis is separation, identifies, the common technology of purification of nucleic acid molecules, is in nucleic acids research work
Indispensable important analysis means, are widely used in fundamental research, agricultural sciences, medical hygiene, industrial production, national defence
The multiple fields such as scientific research, medical jurisprudence and commodity inspection play an important roll in the teaching and scientific research of biochemistry and molecular biology.
Agarose nucleic acid gel electrophoresis can be such that negatively charged nucleic acid molecules are migrated to anode in electrophoresis, in electrophoresis mistake
It needs to use Ago-Gel as electrophoresis supporting dielectric in journey, plays molecular sieve function so that the size core different with conformation
There is larger difference in the mobility of acid molecule, to achieve the purpose that separation, so the quality of Ago-Gel decides entirely
The successful progress of gel electrophoresis experiment, is the basis of nucleic acids research.
Traditional Ago-Gel makes basic program:It chooses mold, configuration glue, colloidal sol, nucleic acid be added
Coloring agent, fall glue, stand solidification, although make principle, method it is simple, in its operating process there are still some problems
It needs to overcome and solve:(1) the nucleic acid staining agent being added in operating process has certain toxicity, experimenter's Long Term Contact nucleic acid dye
Material has higher carcinogenic risk, seriously affects experimenter's health, pollutes environment;(2) due to gel in real process
Powder be added number, amount of liquid number, all there is artificial subjective factor, lack standardization, also lead in temperature control etc.
Experiment electrophoretic image is caused, difference occurs, influences data analysis;(3) entire manufacturing process be all by manual operation, it is time-consuming, laborious,
And artificial error can influence the whole progress of experiment toward contact.
Invention content
The problem to be solved in the present invention is to be to provide gel preparation automation equipment, avoids people and is contacted with reagent,
Security system is promoted, while realizing automation control, saves manpower, labor intensity is reduced, has saved the preparation time of gel,
It improves work efficiency, it is easy to use and reliable.
In order to solve the above technical problems, the technical solution adopted by the present invention is:Gel preparation automation equipment, including it is solidifying
The upper end of glue bottle, the gel bottle is equipped with inlet tube and dye applicator that the import with the gel bottle is correspondingly arranged, the gel
Bottle can move up and down, and the gel bottle can be rotated along horizontal axis that is vertical with its own axis and intersecting, the gel
The lower end of bottle is equipped with heating plate and gel mold.
Further, the middle and lower part of gel bottle outer ring is equipped with multiple cooling fins, and the outer ring of the gel bottle is equipped with temperature
Inductor is spent, the temperature inductor is electrically connected with control centre.
Further, the gel bottle includes the entrance on top and the fusion section of lower part, the entrance be opening to
On it is tubaeform, the outlet end of the inlet tube and the dye applicator is located within the scope of the section of the entrance, the fusion
Section is the cone that diameter becomes larger, and the lower end of the fusion section is equipped with fillet.
Further, the middle and upper part of the gel bottle is installed on holding frame, and the holding frame is installed in electric rotating machine
Output shaft end, the electric rotating machine is located on movable stand and the relatively described movable stand slides up and down connection.
Further, side of the movable stand far from the electric rotating machine is equipped with lifting motor, and the lifting motor is logical
Silk rolling master screw mechanism drives the electric rotating machine to be slided up and down with the movable stand and connect, and the lower end of the crane passes through company
Fishplate bar horizontally slips with support column and connect, and the side of the support column is equipped with mobile motor, and the mobile motor passes through screw silk
Bumper structure drives the connecting plate movement.
Further, the gel mold is bathtub construction, and multiple partition boards that are internally provided with of the gel mold will be described
The inside division of gel mold is multiple independent spaces, and one end of each partition board upper surface is equipped with flow-guiding mouth, multiple water conservancy diversion
The setting sequence of mouth is the sequence that gel passes sequentially through each independent space.
Further, two flow-guiding mouths in a separate space are diagonally arranged, and the height of the flow-guiding mouth is 1~7mm,
The lower end of the gel mold is equipped with semiconductor coldplate, and the semiconductor coldplate is electrically connected with control centre, the gel
The side of mold is equipped with radio frequency identification module, and the radio frequency identification module is electrically connected with the control centre.
Further, the lower end of the gel mold is equipped with fixed plate, and the side of the fixed plate is equipped with pushing piece, described
The force-output shaft of pushing piece is fixedly connected with the fixed plate, and the both sides in the dead plate upper end face are equipped with positioning column, the gel
Mold is located in the symmetrically arranged positioning column, and the both sides of the fixed plate are equipped with guide post, the axis of the guide post with
The axis of the pushing piece is arranged in parallel.
Further, the dye applicator is injection needle construction, and the dye applicator includes syringe needle, needle tubing and piston, the needle
The outlet end of head is arranged in the upper end of gel bottleneck, the piston and driven gear screw-thread fit, is engaged with the driven gear
Setting is driving gear, and the driving gear is rotated by driving motor, and the fixed part of the needle tubing and device, which is fixed, to be connected
It connects.
Further, the inlet tube is connected to liquid storage bottle, and the liquid storage bottle is located in bottle stand, the outside of the inlet tube
Equipped with automatic liquid supply batcher, the automatic liquid supply batcher is connect with control centre, is equipped between the inlet tube and storage bottle
Peristaltic pump.
Method using gel preparation automation equipment is as follows:
The first step:It determines gel proportions and size, weighs a certain amount of gel powder and be added in gel bottle, control centre opens
It is dynamic, size of the automatic liquid supply batcher according to gel, concentration automatic liquid-feeding;
Second step:Holding frame captures gel bottle or so or rotation concussion mixing, and gel bottle is moved into heating plate, coagulates
Glue bottle is brought rapidly up to the temperature for matching gel needs;
Third walks:By semiconductor chilling plate and external exhaust fan synergistic effect, extremely by the coagulant liquid fast cooling of heating
Preference temperature needed for later stage dyestuff or other substances, while the gas being discharged is absorbed and filter by adsorption layer, to reduce
Pollution of the noxious material to ambient enviroment;
4th step:Start dye applicator, dying agent or other reagents are added, piston motion is driven by driving motor, or
It drives piston motion to complete the effect of reagent adding by stepper motor, or microsyringe is used to complete reagent adding;
5th step:Gel mold is put into fixed plate, gel mold is pre-chilled base semiconductor cooling piece, same to alarm
Boom unit, reminds whether gel slab is placed with whether position is proper;
6th step:Electric rotating machine starts, and after the substance mixing in gel bottle, gel bottle is moved to the upper of gel mold
Side, is poured into gel mold;
7th step:Cool down after gel condensation, gel mold pops up under the action of pushing piece, pass hull closure power supply.
Compared with prior art, the invention has the advantages and positive effects that:1, whole device according to setting parameter
It is automatic to carry out, reduce manual steps, gel prepares more standardized, greatly improves the success rate of glue, glue process
Synthetic gel's powder is only needed to weigh, the complete instrument of other steps is completed, and is not necessarily to personnel's watch operation, is reduced connecing for noxious material
It touches, reduces the risk of operating personnel's unexpected injury, and structure, using simplicity, automation, intelligence degree are high, improve gel
The efficiency of preparation;2, be arranged dye applicator, it can be achieved that dyestuff or other reagents automatic addition, reduce experimenter's Long Term Contact core
Sour coloring agent, reduce carcinogenic risk, prevent influence experimenter's health, prevent pollution environment, improve safety in utilization and
Convenience, and save manpower by control centre's upper charging automatically using driving motor, promote working efficiency;3, setting rotation
Rotating motor, lifting motor and mobile motor realize moving up and down and rotating for gel bottle, ensure that heating, rock with
And the function of glue, high degree of automation, labor intensity is reduced, while improving working efficiency;4, in the outer ring of gel bottle
Temperature inductor is set, moves fluid infusion batcher in the external setting of liquid storage bottle, temperature is controlled and logical to the control of liquid volume added
Control centre's realization is crossed, artificial subjective factor is reduced, improves standardization, prevents experiment electrophoretic image from difference occur, prevents from influencing
Receipt is analyzed, and is reduced and is used limitation, and without human intervention, time saving and energy saving, prevents manual operation from generating error influence real
Whole progress is tested, practicability is improved;5, exhaust fan is set and adsorption layer can be timely when toxic gas is accidentally revealed
Handled and adsorbed, ensure to operator's safety while, protect environment, the feature of environmental protection is stronger, safety coefficient higher.
Description of the drawings
The attached drawing for constituting the part of the present invention is used to provide further understanding of the present invention, schematic reality of the invention
Example and its explanation are applied for explaining the present invention, is not constituted improper limitations of the present invention.In the accompanying drawings:
Fig. 1 is the structural schematic diagram that inventive gel preparation is faced with automation equipment;
Fig. 2 is the structural schematic diagram of inventive gel preparation automation equipment left view;
Fig. 3 is the structural schematic diagram of inventive gel preparation automation equipment backsight;
Fig. 4 is dye applicator of the present invention and drives the structural schematic diagram of its correlated parts moved;
Fig. 5 is the structural schematic diagram of the embodiment of the present invention 2;
Fig. 6 is the structural schematic diagram of the embodiment of the present invention 3.
Reference numeral:
1- gel bottles;11- cooling fins;12- temperature inductors;13- entrances;14- merges section;2- inlet tubes;21- liquid storages
Bottle;211- bottle stands;22- peristaltic pumps;23- automatic liquid supply batchers;3- dye liquor rifles;31- syringe needles;32- needle tubings;33- pistons;34-
Driven gear;35- driving gears;36- driving motors;37- linear potentiometers;38- struts;381- guide grooves;39- stepping electricity
Machine;4- heating plates;5- gel molds;51- radio frequency identification modules;52- partition boards;53- flow-guiding mouths;6- control centres;61- control planes
Plate;7- holding frames;71- electric rotating machines;72- movable stands;73- lifting motors;74- connecting plates;75- support columns;76- movement electricity
Machine;8- pushing pieces;81- fixed plates;82- positioning columns;83- guide posts;9- bottom plates;91- exhaust fans;92- adsorption layers.
Specific implementation mode
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.
In the description of the present invention, it is to be understood that, term "center", " longitudinal direction ", " transverse direction ", "upper", "lower",
The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is
It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of description of the present invention and simplification of the description, rather than instruction or dark
Show that signified device or element must have a particular orientation, with specific azimuth configuration and operation, therefore should not be understood as pair
The limitation of the present invention.In addition, term " first ", " second " etc. are used for description purposes only, it is not understood to indicate or imply phase
To importance or implicitly indicate the quantity of indicated technical characteristic.The feature for defining " first ", " second " etc. as a result, can
To express or implicitly include one or more this feature.In the description of the present invention, unless otherwise indicated, " multiple "
It is meant that two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected;It can
Can also be electrical connection to be mechanical connection;It can be directly connected, can also indirectly connected through an intermediary, Ke Yishi
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood by concrete condition
Concrete meaning in the present invention.
Specific embodiments of the present invention will now be described in detail with reference to the accompanying drawings.
As shown in Figure 1, Figure 2 and Figure 3, the present invention is gel preparation automation equipment, including gel bottle 1, gel bottle 1
Upper end is equipped with the inlet tube 2 that the import with gel bottle 1 is correspondingly arranged and dye applicator 3, gel bottle 1 and can move up and down, gel
Bottle 1 can be rotated along horizontal axis that is vertical with its own axis and intersecting, and the lower end of gel bottle 1 is equipped with heating plate 4 and gel mold
Tool 5, the mode of electromagnetic heating can be used in heating plate 4, can also be by the way of canister heating so that in gel bottle 1
Temperature is rapidly heated between 80 to 100 degree, and specific temperature can be adjusted and set according to actual conditions;Preferably, it coagulates
Glue bottle 1 is using vial or the metal bottle of other anticorrosion materials.
Preferably, the middle and lower part of 1 outer ring of gel bottle is equipped with multiple cooling fins 11, and the height of cooling fin 11 is more than gel bottle height
The 1/2 of degree, multiple cooling fins 11 are distributed on the outer ring of gel bottle 1, the thickness of cooling fin 11 and the close setting of the thickness of gel bottle,
The quantity of cooling fin 11 is more as possible, can promote the speed to cool down in gel process, shortens gel process, the outer ring of gel bottle 1 is set
There are temperature inductor 12, temperature inductor 12 to be electrically connected with control centre 6, temperature inductor 12 is located in multiple cooling fins 11
Between, this region is the height region at the main place of material mixing, and the temperature being arranged inside the gel bottle 1 that this region incudes is more
Add it is accurate, after the temperature value for reaching setting, control centre 6 control again electric rotating machine 71 carry out gel bottle 1 stopping vibration and
The actions such as rotary dumping;It is highly preferred that gel bottle 1 includes the fusion section 14 of the entrance 13 and lower part on top, entrance 13 is
Opening upwards it is tubaeform, facilitate charging, inlet tube 2 and the outlet end of dye applicator 3 are located within the scope of the section of entrance 13, into
Be arranged straight down after the discharging of liquid pipe 2, fusion section 14 is the cone that becomes larger of diameter, below it is big small above, prevent in
The substance in portion splashes out during shaking, and environmental protection, the feature of environmental protection is strong, while also avoiding substance from leaking and being made safely to operator
It to avoid contact with and leak because dyestuff has certain toxicity at influence, the lower end of fusion section 14 is equipped with fillet, using circle
Angle facilitates internal substance fully to merge, and avoids the occurrence of dead angle.
Preferably, the middle and upper part of gel bottle 1 is installed on holding frame 7, can connect the centre of entrance 13 and fusion section 14
Socket part position is located on holding frame 7, because position is the most thin position of diameter thus, is fixed conveniently, and be not easy play up and down, folder
The output shaft end that frame 7 is installed in electric rotating machine 71 is held, electric rotating machine 71 is connect with control centre 6, the injected material in gel bottle 1
After need constantly to rock gel bottle 1, ensure the abundant fusion of inner material, and electric rotating machine 71 can control gel
Bottle 1 goes out gum forming, electric rotating machine 71, which is located on movable stand 72 and relatively moves frame 72, slides up and down connection, up and down towards setting
After movement, it is ensured that gel bottle 1 is fallen in heating plate 4, is heated to internal substance, realizes substance in gel bottle 1
Be rapidly heated, promote the formation of gel;It is highly preferred that side of the movable stand 72 far from electric rotating machine 71 is equipped with lifting motor
73, lifting motor 73 is slided up and down with movable stand 72 by screw screw drive mechanism electric rotating machine 71 and is connect, under crane
End, which is horizontally slipped by connecting plate 74 with support column 75, to be connect, and the side of support column 75 is equipped with mobile motor 76, mobile motor 76
Plate 74 is drivingly connected by screw screw structure to move, the setting of mobile motor 76 can the drive shifting of movable stand 72 or so of connecting plate 74
It is dynamic, and then moving left and right for gel bottle 1 is realized, gel mold 5 and heating plate 4, heating plate is arranged simultaneously in the lower end of gel bottle 1
4 and gel mold 5 be arranged in parallel, therefore gel bottle 1 needs to move between, it is therefore desirable to which left and right is certain, electric rotating machine
71 realize the action rocked vibration and topple over into gel to gel bottle 1, and lifting motor 73 realizes the upper and lower of gel bottle 1
Lifting ensure that the decline of gel bottle 1 is contacted with heating plate 4, rise the total mistake detached with heating plate 4, mobile motor 76 realizes
The left and right of gel is certain, realizes movement of the gel between heating plate 4 and gel mold 5, and total can be realized upper and lower, left
Right and rotation multiple directions actions, can be achieved the above automation control acted by the setting of control centre 6, are promoted automatic
Change degree.
It is highly preferred that side of the support column 75 far from gel bottle 1 is equipped with exhaust fan 91, between exhaust fan 91 and support column 75
Equipped with adsorption layer 92, active carbon layer or chemisorbed layer 92 can be selected in adsorption layer 92, if any gas overflowing, in the work of exhaust fan 91
Under, gas can be adsorbed quickly by adsorption layer 92, avoid bad air leakage from causing environmental pollution, it is highly preferred that whole knot
The outside of structure is equipped with protective cover, outlet is arranged in the position of 1 gel of gel bottle, this process realizes automation control, further
Security performance is promoted, and the setting of exhaust fan 91 can further speed up the cooling of gel bottle 1, promote the speed of gel.
Preferably, gel mold 5 is bathtub construction, and gel mold 5 is internally provided with multiple partition boards 52 by gel mold 5
Inside division is multiple independent spaces, and one end of 52 upper surface of each partition board is equipped with flow-guiding mouth 53, multiple flow-guiding mouths 53
Setting sequence is the sequence that gel passes sequentially through each independent space, during gel, is ensured independent empty at first
Between Gel Height reach the height of setting after second separate space is flowed to by flow-guiding mouth 53 again, carry out successively, until more
A equal gel in space is completed, and gel can be sized to different specifications, meet the needs of different tests, Er Qiekong by this structure
Between size can be split, even if sometimes gel content it is small if can guarantee reach part separate space gel setting, shape
Specification facilitates storage and follow-up use;It is highly preferred that two flow-guiding mouths 53 in a separate space are diagonally arranged, phase is avoided
When neighbour's setting, the gel of the separate space at place just has partial gel to flow to next independence after not reaching pre-provisioning request also
Space in, the height of partition board 52 be 10mm hereinafter, flow-guiding mouth 53 upper surface to 5 the lowermost height of gel mold be more than
Equal to 3mm, the height of flow-guiding mouth 53 is 1~7mm, ensures good mobility in gel process, the lower end of gel mold 5 is equipped with
Semiconductor coldplate, semiconductor coldplate are electrically connected with control centre 6, accelerate cooling, contracting during helping to realize gel
The time of short gel, the efficiency of gel is promoted, the side of gel mold 5 is equipped with radio frequency identification module 51, radio frequency identification module 51
It is electrically connected with control centre 6, chip information is read by radio frequency identification module 51, judges whether 5 position of gel mold puts well, class
Whether type is correct, and controls alarm buzzer and send out alarm sound, prompts operator to continue operation and still needs to correct, whether type
Correct is exactly the gel shape of preparation making, i.e., it is corresponding whether the shape of above-mentioned separate space carries out with the outlet end of gel bottle 1,
According to different situations, the position for adjusting gel mold 5 may be needed every time, determines the position of the first gel space, adjust solidifying
The flow direction etc. of glue.
Preferably, the lower end of gel mold 5 is equipped with fixed plate 81, and the side of fixed plate 81 is equipped with pushing piece 8, pushing piece 8
It is at low cost using the structure of electric pushrod or cylinder, preferably electric pushrod, easy-to-connect, the force-output shaft of pushing piece 8 with it is solid
Fixed board 81 is fixedly connected, and the force-output shaft of pushing piece 8 does the action extended and retracted, you can realize the movement of fixed plate 81, it is fixed
The both sides of 81 upper surface of plate are equipped with positioning column 82, and gel mold 5 is located in symmetrically arranged positioning column 82, to gel mold 5 into
Row positioning, ensures in 81 moving process of fixed plate, the stability of gel mold 5, and the both sides of fixed plate 81 are equipped with guide post 83, lead
It is arranged in parallel to the axis of the axis and pushing piece 8 of column 83, ensure that the precision that fixed plate 81 moves, push the axis and branch of frame
The longitudinal center plane of dagger 75 is vertically arranged.
Preferably, as shown in figure 4, dye applicator 3 is injection needle construction, dye applicator 3 includes syringe needle 31, needle tubing 32 and piston
33, the outlet end of syringe needle 31 is arranged in the upper end of 1 mouthful of gel bottle, piston 33 and 34 screw-thread fit of driven gear, with driven gear
34 engagement settings are driving gear 35, and driving gear 35 is rotated by driving motor 36, the fixed part of needle tubing 32 and device
It is fixedly connected, needle tubing 32 is fixed on the upper end of peristaltic pump 22, saves space, and the outside of needle tubing 32 is equipped with scale, is conveniently operated people
Member proceeding by dyestuff injection when carry out use state estimation, other substances can also be injected, as antibiotic, enzyme,
Chemical reagent etc., when needing to inject dyestuff, control centre 6 controls driving motor 36 and rotates, and then driving gear 35 drives
Driven gear 34 rotates, because driven gear 34 is threadedly coupled with piston 33, during driven gear 34 rotates, and 33 meeting of piston
It pushes ahead, completes the automatic injection of dyestuff;It is highly preferred that the one end of piston 33 far from syringe needle 31 is equipped with linear potentiometer 37,
Straight-line electric draws device to be fixed on link, and link is arranged on peristaltic pump 22;It is highly preferred that perfusion syringe needle 31 oozes every time
It need to be supplemented manually when 20ul, internal liquid residue 0.5ml.
Preferably, inlet tube 2 is connected to liquid storage bottle 21, and liquid storage bottle 21 is located in bottle stand 211, and bottle stand 211 is located at bottom plate 9
On, bottle stand 211 is the barrel-shaped of opening upwards, and liquid storage bottle 21, which is directly placed in bottle stand 211, can prevent liquid storage bottle 21 from shaking, feed liquor
The outside of pipe 2 is equipped with automatic liquid supply batcher 23, and automatic liquid supply batcher 23 connect with control centre 6, inlet tube 2 and storage bottle it
Between be equipped with peristaltic pump 22, when needing to squeeze into liquid, control centre 6 control peristaltic pump 22 act, liquid by inlet tube 2 into
Enter into gel bottle 1, inlet tube 2 uses rubber hose, heating plate 4 to be located at the both sides of gel bottle 1 with peristaltic pump 22;More preferably
Ground, all two pieces are each attached on bottom plate 9 so that structure constitutes a skid structure, need to only assemble it is primary, each part it
Between relative position relation i.e. can guarantee, facilitate it is subsequent transhipment and move.
Control centre 6 includes micro-control microcontroller, control panel 61, micro-control microcontroller and peristaltic pump 22, electric rotating machine
71, lifting motor 73, mobile motor 76, driving motor 36, pushing piece 8, radio frequency identification module 51 and control panel 61 connect, and connect
The input signal of control panel 61 is received, and to peristaltic pump 22, electric rotating machine 71, lifting motor 73, mobile motor 76, driving motor
36, pushing piece 8, radio frequency identification module 51 are controlled, and micro-control microcontroller is data processing switching centre, for receiving, analyzing,
Processing, transmission data and instruction, control panel 61 are used for input control parameter or outputting operating data.
Embodiment 2:As shown in figure 5, other structures are same as described above, unlike, dye applicator 3 is microsyringe knot
Structure, dye applicator 3 are located at by strut 38 on bottom plate 9, and strut 38 is located at side of the heating plate 4 far from gel bottle 1, and pedestal upper end is set
Guide groove 381 is set, dye applicator 3 is located in guide groove 381 and is arranged diagonally downward.
Embodiment 3:As shown in fig. 6, other structures are same as Example 2, the difference is that dye applicator 3 is injection needle construction,
Dye applicator 3 includes syringe needle 31, needle tubing 32 and piston 33, and the outlet end of syringe needle 31 is arranged in the upper end of 1 mouthful of gel bottle, piston 33 with
Stepper motor 39 connects, and completes dyestuff or the injection of other substances.
Method using gel preparation automation equipment is as follows:
The first step:It determines gel proportions and size, weighs a certain amount of gel powder and be added in gel bottle 1, control centre 6
Start, size of the automatic liquid supply batcher 23 according to gel, concentration automatic liquid-feeding;
Second step:Holding frame 7 captures gel bottle 1 or so or rotation concussion mixing, and gel bottle 1 is moved into heating plate 4
On, gel bottle 1 is brought rapidly up to the temperature for matching gel needs;
Third walks:It is acted synergistically by semiconductor chilling plate and external exhaust fan 91, by the coagulant liquid fast cooling of heating
To the preference temperature needed for later stage dyestuff or other substances, while the gas being discharged is absorbed and filter by adsorption layer 92, with
Reduce pollution of the noxious material to ambient enviroment;
4th step:Start dye applicator 3, dying agent or other reagents are added, piston motion is driven by driving motor 36, or
Person drives piston motion to complete the effect of reagent adding by stepper motor 39, or microsyringe is used to complete reagent adding;
5th step:Gel mold 5 is put into fixed plate 81, gel mold 5 is pre-chilled base semiconductor cooling piece, simultaneously
Alarm boom unit, reminds whether gel mold 5 is placed with whether position is proper;
6th step:Electric rotating machine 71 starts, and after the substance mixing in gel bottle 1, gel bottle 1 is moved to gel mold
5 top is poured into gel mold 5;
7th step:Cool down after gel condensation, gel mold 5 pops up under the action of pushing piece 8, pass hull closure power supply.
It in practical work process, is carried out in strict accordance with above step, gel accuracy is high, efficient, product qualification rate
Height, operation is not error-prone, presses gel proportions and size first, weighs a certain amount of gel powder and be added in gel bottle 1, then make
It is inputted and is instructed with control panel 61, controlling peristaltic pump 22 by micro-control microcontroller acts, and passes through automatic liquid supply batcher 23 and root
Automatic liquid-feeding is carried out according to the size of gel, ratio, then opens electric rotating machine 71, driving gel bottle 1 rotates concussion mixing, mixing
Lifting motor 73 and mobile motor 76 act simultaneously afterwards, and gel bottle 1 is placed into heating plate 4, gel bottle 1 be brought rapidly up to
100 DEG C, after the completion of heating, lifting motor 73 and mobile motor 76 act, while gel bottle 1 is lifted off heating plate 4, from adding
The top that the side of hot plate 4 moves to gel mold 5 is cooled down, and semiconductor coldplate, cooling fin 11 and exhaust fan are passed through
91 synergistic effects, the preference temperature needed for coagulant liquid fast cooling to dyestuff by heating, while the gas being discharged passes through activity
Charcoal is absorbed and filter, and dye applicator 3 starts, and piston 33 pushes, and dyestuff or other reagents is injected in coagulant liquid, at this time gel mold
Tool 5 is placed into fixed plate 81, and gel mold 5 is pre-chilled base semiconductor coldplate, and core is read by radio frequency identification module 51
Piece information, judges whether 5 position of gel mold puts well, and coagulant liquid will by holding frame 7 after shaking mixing by electric rotating machine 71
After gel bottle 1 is moved to the top of gel slab, poured into gel mold 5 after opening rotation downwards, after final gel condensation, gel
Mold 5 is released by pushing piece 8, gel complete, pass hull closure power supply, whole device according to setting parameter automatically into
Row, reduces manual steps, and gel prepares more standardized, greatly improves the success rate of glue, glue process only needs people
Work gel powder weighs, and the complete instrument of other steps is completed, and is not necessarily to personnel's watch operation, is reduced the contact of noxious material, is reduced
The risks of operating personnel's unexpected injuries, and structure, using easy, automation, intelligence degree are high, improve prepared by gel
Efficiency.
One embodiment of the present invention has been described in detail above, but the content be only the present invention preferable implementation
Example should not be construed as limiting the practical range of the present invention.It is all according to all the changes and improvements made by the present patent application range
Deng should all still fall within the scope of the patent of the present invention.
Claims (10)
1. gel preparation automation equipment, it is characterised in that:Including gel bottle, the upper end of the gel bottle is equipped with coagulates with described
The inlet tube and dye applicator that the import of glue bottle is correspondingly arranged, the gel bottle can move up and down, the gel bottle can along with
Its own axis is vertically and the rotation of the horizontal axis of intersection, the lower end of the gel bottle are equipped with heating plate and gel mold.
2. gel preparation automation equipment according to claim 1, it is characterised in that:Under in gel bottle outer ring
Portion is equipped with multiple cooling fins, and the outer ring of the gel bottle is equipped with temperature inductor, and the temperature inductor is electrically connected with control centre
It connects.
3. gel preparation automation equipment according to claim 1 or 2, it is characterised in that:The gel bottle includes upper
The entrance in portion and the fusion section of lower part, the entrance are the tubaeform of opening upwards, the inlet tube and the dye applicator
Outlet end be located within the scope of the section of the entrance, the fusion section is the cone that becomes larger of diameter, the fusion
The lower end of section is equipped with fillet.
4. according to claims 1 or 2 any one of them gel preparation automation equipment, it is characterised in that:The gel bottle
Middle and upper part be installed on holding frame, the holding frame is installed in the output shaft end of electric rotating machine, and the electric rotating machine is located at shifting
In moving frame and the relatively described movable stand slides up and down connection, and side of the movable stand far from the electric rotating machine is equipped with lifting electricity
Machine, the lifting motor is slided up and down with the movable stand by electric rotating machine described in screw screw drive mechanism and is connect, described
The lower end of crane, which is horizontally slipped by connecting plate with support column, to be connect, and the side of the support column is equipped with mobile motor, described
Mobile motor drives the connecting plate to move by screw screw structure.
5. according to claims 1 or 2 any one of them gel preparation automation equipment, it is characterised in that:The gel mold
Tool be bathtub construction, the gel mold be internally provided with multiple partition boards by the inside division of the gel mold be multiple independences
Space, one end of each partition board upper surface is equipped with flow-guiding mouth, and the setting sequence of multiple flow-guiding mouths is that gel passes sequentially through often
The sequence in a independent space.
6. gel preparation automation equipment according to claim 5, it is characterised in that:Two in one separate space
Flow-guiding mouth is diagonally arranged, and the height of the flow-guiding mouth is 1~7mm, and the lower end of the gel mold is equipped with semiconductor coldplate, institute
It states semiconductor coldplate to be electrically connected with control centre, the side of the gel mold is equipped with radio frequency identification module, and the radio frequency is known
Other module is electrically connected with the control centre.
7. gel preparation automation equipment according to claim 6, it is characterised in that:The lower end of the gel mold is set
There are fixed plate, the side of the fixed plate to be equipped with pushing piece, the force-output shaft of the pushing piece is fixedly connected with the fixed plate, institute
The both sides for stating dead plate upper end face are equipped with positioning column, and the gel mold is located in the symmetrically arranged positioning column, described solid
The both sides of fixed board are equipped with guide post, and the axis of the guide post and the axis of the pushing piece are arranged in parallel.
8. according to claim 1,2,6 or 7 any one of them gel preparation automation equipments, it is characterised in that:The dye
Spray gun is injection needle construction, and the dye applicator includes syringe needle, needle tubing and piston, and the outlet end of the syringe needle is arranged in gel bottleneck
Upper end, the piston and driven gear screw-thread fit engage that be arranged be driving gear with the driven gear, the active
Gear is rotated by driving motor, and the needle tubing is fixedly connected with the fixed part of device.
9. according to claim 1,2,6 or 7 any one of them gel preparation automation equipments, it is characterised in that:It is described into
Liquid pipe is connected to liquid storage bottle, and the liquid storage bottle is located in bottle stand, and the outside of the inlet tube is equipped with automatic liquid supply batcher, described
Automatic liquid supply batcher is connect with control centre, and peristaltic pump is equipped between the inlet tube and storage bottle.
10. using the method for gel preparation automation equipment described in claim 1, it is characterised in that:
The first step:It determining gel proportions and size, weighs a certain amount of gel powder and be added in gel bottle, control centre starts,
Size of the automatic liquid supply batcher according to gel, concentration automatic liquid-feeding;
Second step:Holding frame captures gel bottle or so or rotation concussion mixing, and gel bottle is moved into heating plate, gel bottle
It is brought rapidly up to the temperature for matching gel needs;
Third walks:By semiconductor chilling plate and external exhaust fan synergistic effect, by the coagulant liquid fast cooling of heating to later stage
Preference temperature needed for dyestuff or other substances, while the gas being discharged is absorbed and filter by adsorption layer, it is toxic to reduce
Pollution of the substance to ambient enviroment;
4th step:Start dye applicator, dying agent or other reagents are added, piston motion is driven by driving motor, or pass through
Stepper motor drives piston motion to complete the effect of reagent adding, or completes reagent adding using microsyringe;
5th step:Gel mold is put into fixed plate, gel mold is pre-chilled base semiconductor cooling piece, with alarm buzzing
Device, reminds whether gel slab is placed with whether position is proper;
6th step:Electric rotating machine starts, and after the substance mixing in gel bottle, gel bottle is moved to the top of gel mold,
It pours into gel mold;
7th step:Cool down after gel condensation, gel mold pops up under the action of pushing piece, pass hull closure power supply.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810615436.XA CN108459068B (en) | 2018-06-14 | 2018-06-14 | Automatic device for gel preparation and use method |
KR1020207036992A KR20210003291A (en) | 2018-06-14 | 2018-06-21 | Gel manufacturing automation device and method of use |
PCT/CN2018/092086 WO2019237409A1 (en) | 2018-06-14 | 2018-06-21 | Automatic device for gel preparation and usage method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810615436.XA CN108459068B (en) | 2018-06-14 | 2018-06-14 | Automatic device for gel preparation and use method |
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CN108459068A true CN108459068A (en) | 2018-08-28 |
CN108459068B CN108459068B (en) | 2024-04-30 |
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CN201810615436.XA Active CN108459068B (en) | 2018-06-14 | 2018-06-14 | Automatic device for gel preparation and use method |
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KR (1) | KR20210003291A (en) |
CN (1) | CN108459068B (en) |
WO (1) | WO2019237409A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111545138A (en) * | 2020-05-07 | 2020-08-18 | 广州博鹭腾生物科技有限公司 | Method for rapidly preparing gel and automatic gel preparation system |
CN114379055A (en) * | 2021-11-05 | 2022-04-22 | 湖北天霖新材料有限公司 | Polyethylene double-wall corrugated pipe forming equipment |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114965657B (en) * | 2022-06-07 | 2023-11-24 | 苏州科技大学 | Automatic system and device for preparing glue by using biological electrophoresis polymer |
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Also Published As
Publication number | Publication date |
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CN108459068B (en) | 2024-04-30 |
KR20210003291A (en) | 2021-01-11 |
WO2019237409A1 (en) | 2019-12-19 |
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