CN206710374U - A kind of automatic glue instrument of new Ago-Gel - Google Patents
A kind of automatic glue instrument of new Ago-Gel Download PDFInfo
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- CN206710374U CN206710374U CN201720190892.5U CN201720190892U CN206710374U CN 206710374 U CN206710374 U CN 206710374U CN 201720190892 U CN201720190892 U CN 201720190892U CN 206710374 U CN206710374 U CN 206710374U
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Abstract
The utility model discloses a kind of new automatic glue instrument of Ago-Gel, it is related to gel instrument field.The new automatic glue instrument of Ago-Gel includes instrument body, sets slide rail on instrument body, connection support bar on slide rail, and support bar connects vertical rod by lifting module, and agitating paddle is set on vertical rod.Shuttle is set below agitating paddle, and shuttle is placed on heating module and liquid bleeder is set on shuttle, and temperature controlled region and elevating control area are set in front of instrument body.The utility model passes through the optimization design to instrument, it ensure that the simple for production of Ago-Gel, reduce the personnel to operate link, pass through the design of temperature control device, when avoiding off-line working, because of the phenomenon that agarose temperature reduces and solidifies, while by the control to temperature, reduce the risk scalded when personnel weigh agarose temperature with hand in DNA dyestuff adding procedures.
Description
Technical field
Gel instrument field is the utility model is related to, specially a kind of automatic glue instrument of new Ago-Gel.
Background technology
Agarose gel electrophoresis is a kind of electrophoresis method for making supporting dielectric with agarose.Its analysis principle and other supports
Thing electrophoresis it is main difference be：It has the double action of " molecular sieve " and " electrophoresis " concurrently.
Ago-Gel has a network structure, material molecule by when can by resistance, macromolecular substances when springing up by
The resistance arrived is big, therefore in gel electrophoresis, the separation of charged particle depends not only on the property and quantity of net charge, but also
Depending on molecular size, this has been considerably improved resolution capability.But because its aperture is too big compared to protein, to most of eggs
Its molecular sieving effect is insignificant for white matter, is now widely used in the research of nucleic acid.
Protein can carry different electric charges with nucleic acid according to pH is different, and stress is of different sizes in the electric field, therefore the speed run
Degree is different, can be separated around this principle.The pH of electrophoretic buffer is between 6 ~ 9, and ionic strength 0.02 ~ 0.05 is most
It is suitable.Conventional 1% agarose is as electrophoresis holder.Ago-Gel can about distinguish difference 100bp DNA fragmentation, its resolution ratio
Though lower than polyacrylamide gel, it is easy that it is prepared, and separating ranges are wide.Plain agar sugar gel separation DNA scope be
0.2-20kb, using pulse electrophoresis, separate the DNA fragmentation for being up to 10^7bp.
DNA molecular has charge effect and molecular sieving effect in Ago-Gel during swimming.DNA molecular is higher than isoelectric point
PH solution in it is negatively charged, in the electric field to positive pole move.It is identical due to repetitive nature of the sugar-phosphate backbones in structure
The double-stranded DNA of quantity almost has the net charge of equivalent, therefore they can be moved to positive extreme direction at the same rate.
Complete by human users when current Ago-Gel prepares main, not only waste time and energy, and if gel
When concentration is higher, simple heating can not allow agarose to be thoroughly dissolved in electrophoretic buffer, need to also be in agar in DNA electrophoresis
Sugar, which is cooled to after a certain temperature, adds dyestuff into gel solution, needs experimenter to pass through extremity to push away during manual operation
The temperature of gel solution is surveyed, this just causes inconvenient and certain danger to experimental implementation, and DNA dyestuffs are hypertoxicities, also can be right
Operating personnel bring very big danger.In gel temperature-fall period, generally, experimenter is required for standby, ensures it
Temperature can not allow colloid to condense while decline, and also experiment can be brought inconvenience.
The content of the invention
（One）The technical problem of solution
In view of the shortcomings of the prior art, the utility model provides a kind of automatic glue instrument of new Ago-Gel.Solve
The problem of gel automatic heat preserving, reduce human users, avoid gel and be prepared as the danger that experimenter brings.
（Two）Technical scheme
To realize object above, the utility model is achieved by the following technical programs：A kind of new Ago-Gel
Automatic glue instrument, including instrument body, set slide rail on instrument body, and connection support bar on slide rail, support bar is by lifting mould
Block connects vertical rod, and agitating paddle is set on vertical rod.Shuttle is set below agitating paddle, and shuttle is placed on heating module
Liquid bleeder above and on shuttle is set, temperature controlled region and elevating control area are set in front of instrument body.Preferably, institute
Unidirectional slide rail is set on the primer box stated, the buckle between slide rail and sliding block uses triangular construction.
Preferably, slide rail is set on instrument body, and connection support bar on slide rail, support bar is vertical by lifting module connection
Bar, agitating paddle is set on vertical rod.
Preferably, shuttle is set below agitating paddle, and shuttle is placed on heating module and on shuttle
Liquid bleeder is set.
Preferably, temperature controlled region and elevating control area are set in front of instrument body.
（Three）Beneficial effect
The utility model provides a kind of automatic glue instrument of new Ago-Gel, possesses following effective：
（1）, the new automatic glue instrument of Ago-Gel, by the design to slide rail, allow experimenter according to solidifying
The size of colloid product, adjust the height of agitating paddle.
（2）, the new automatic glue instrument of Ago-Gel, by the design to agitating paddle and heating module, make agarose
Gel is also heated while stirring, and it is more uniform to have reached gel solution, dissolving more fully purpose.
（3）, the new automatic glue instrument of Ago-Gel, by the design to heating module and liquid bleeder, make
Gel can be incubated at 60 DEG C after abundant dissolving, effectively reduce the standby work of personnel, while also make experimenter
It is not required to limbs touching be speculated again the temperature of gel solution when adding DNA dyestuffs, reduces risk.
Brief description of the drawings
Accompanying drawing 1,2 is structural representation of the present utility model.
In figure：（1）Instrument body,（2）Vertical rod,（3）Liquid bleeder,（4）Heating module,（5）Agitating paddle,（6）
Controlled valve,（7）Shuttle,（8）Support bar,（9）Lifting module,（10）Temperature controlled region,（11）Elevating control area.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Fig. 1,2 are referred to, the utility model provides a kind of technical scheme：A kind of automatic glue instrument of new Ago-Gel,
Including instrument body 1, slide rail is set, connection support bar 8 on slide rail, support bar 8 hangs down by lifting the connection of module 9 on instrument body
Straight-bar 2, agitating paddle 5 is set on vertical rod 2, and the design of said apparatus ensure that operating personnel can be by the size of gel volume
Carry out the autonomous height for selecting agitating paddle.
The instrument body 1 sets agitating paddle 5, and the lower section of agitating paddle 5 sets shuttle 7, and shuttle 7 is placed on heating
Liquid bleeder 3 is set in module 4 and on shuttle, and the design of said apparatus effectively reduces human users, and makes
Gel solution is constantly stirred simultaneously in heating, makes solution homogeneous heating, agarose dissolving is abundant, while also makes course of dissolution knot
Insulating process after beam realizes temperature control, operating personnel need not standby operation, the DNA dyestuffs for ensureing to add will not be molten because of gel
Liquid temperature is spent high and degraded, and also will not can not fully be mixed because gel solution temperature is too low.
The front of instrument body 1 sets temperature controlled region 10 and elevating control area 11, the design of said apparatus, realizes operation
Personnel can improve grasping for the utility model by independently setting purpose of the instrument parameter so as to personalized customization experimental program
The property made.
In summary, the new automatic glue instrument of Ago-Gel, it is real by the design to agitating paddle 5 and heating module 4
Abundant heating, the mixing to gel solution are showed, and by the design to liquid bleeder, have greatly reduced because of reagent contamination
The injury to caused by personnel.
The new automatic glue instrument of Ago-Gel, by the design to temperature controlled region and elevating control area, meets difference
The requirement of different parameters setting needed for experimental implementation, improve the operability of laboratory apparatus.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply and deposited between these entities or operation
In any this actual relation or order.Moreover, term " comprising ", "comprising" or its any other variant are intended to
Nonexcludability includes, so that process, method, article or equipment including a series of elements not only will including those
Element, but also the other element including being not expressly set out, or it is this process, method, article or equipment also to include
Intrinsic key element.In the absence of more restrictions.By sentence " including one ... the key element limited, it is not excluded that
Other identical element in the process including the key element, method, article or equipment also be present ".
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out with a variety of changes in the case where not departing from principle of the present utility model and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (3)
- A kind of 1. automatic glue instrument of new Ago-Gel, it is characterised in that：The new automatic glue instrument of Ago-Gel, including Instrument body（1）, instrument body（1）Upper setting slide rail（12）, slide rail connection support bar（8）, support bar（8）By lifting module （9）Connect vertical rod（2）, vertical rod（2）On be connected with agitating paddle（5）,Agitating paddle（5）Lower section is shuttle (7), and shuttle (7) is placed on heating module（4）On.
- A kind of 2. new automatic glue instrument of Ago-Gel according to claim 1, it is characterised in that：The shuttle （7）Upper setting liquid bleeder（3）And controlled valve（6）.
- A kind of 3. new automatic glue instrument of Ago-Gel according to claim 1, it is characterised in that：The instrument body （1）Upper setting temperature controlled region（10）With elevating control area（11）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720190892.5U CN206710374U (en) | 2017-03-01 | 2017-03-01 | A kind of automatic glue instrument of new Ago-Gel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720190892.5U CN206710374U (en) | 2017-03-01 | 2017-03-01 | A kind of automatic glue instrument of new Ago-Gel |
Publications (1)
| Publication Number | Publication Date |
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| CN206710374U true CN206710374U (en) | 2017-12-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720190892.5U Active CN206710374U (en) | 2017-03-01 | 2017-03-01 | A kind of automatic glue instrument of new Ago-Gel |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108459068A (en) * | 2018-06-14 | 2018-08-28 | 沧州医学高等专科学校 | Gel preparation automation equipment and application method |
-
2017
- 2017-03-01 CN CN201720190892.5U patent/CN206710374U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108459068A (en) * | 2018-06-14 | 2018-08-28 | 沧州医学高等专科学校 | Gel preparation automation equipment and application method |
| CN108459068B (en) * | 2018-06-14 | 2024-04-30 | 沧州医学高等专科学校 | Automatic device for gel preparation and use method |
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