CN108456641A - The piezoelectric supersonic cutting system and method for egg mother cell oolemma - Google Patents
The piezoelectric supersonic cutting system and method for egg mother cell oolemma Download PDFInfo
- Publication number
- CN108456641A CN108456641A CN201810455757.8A CN201810455757A CN108456641A CN 108456641 A CN108456641 A CN 108456641A CN 201810455757 A CN201810455757 A CN 201810455757A CN 108456641 A CN108456641 A CN 108456641A
- Authority
- CN
- China
- Prior art keywords
- mother cell
- egg mother
- sticking
- oolemma
- micropin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Mechanical Engineering (AREA)
- Surgical Instruments (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of piezoelectric supersonic cutting system of egg mother cell oolemma and method, the piezoelectric supersonic cutting system includes：Egg mother cell pose adjusts chip, for carrying out pose adjusting and bottom fixation sticking to egg mother cell；The end of egg mother cell holding device, including elbow sticking micropin and the micropin clamper for elbow sticking micropin to be clamped, the elbow sticking micropin is unilateral concave circular arc sticking syringe needle, and elbow sticking micropin is internally provided with a bayonet, and end is equipped with a notch；Egg mother cell ultrasonic cutting scalpel, the egg mother cell ultrasonic cutting scalpel are used to carry out feed cutting to egg mother cell oolemma from gap position.The present invention can effectively avoid damaging this disadvantage to egg mother cell due to the longitudinal conductibility of ultrasonic energy, and egg mother cell ultrasonic cutting scalpel cuts oolemma from the tangential direction on egg mother cell surface layer, and can reduce the damage to egg mother cell.
Description
Technical field
The present invention relates to biology microscope operating technology fields, more particularly to a kind of piezoelectric supersonic of egg mother cell oolemma
Cutting system and method.
Background technology
Egg mother cell is most important egg mother cell in life preparation process, and which contain the important heredity of a life entity
Substance, therefore, to the micromanipulative technique of egg mother cell always in occupation of most in life science and relevant medical technology
Important position.As infertile rate is constantly soaring in world wide and the mankind are for the continuous pursuit of prenatal and postnatal care, people
Work auxiliary procreation technology has evolved to third generation tube-test baby techniques, which needs in egg mother cell octoploid phase or blastaea
It burrows on the oolemma of phase embryo, micro catheter is then utilized to take out single germinal layer ovum mother carefully from the eight egg mother cell phases in third day
Born of the same parents carried out identified for genes from the 5th day blastaea access trophoderm egg mother cell, and the embryo operated also needs to survive
And continue to develop.
Currently, there are two types of traditional egg mother cell operating methods：
The first is after being punched to egg mother cell using laser, then is inhaled to substance in egg mother cell with micropin
It takes, though this method experiment effect is good, after being operated to egg mother cell, survival rate is high, pollution-free, and disadvantage is that equipment is high
Expensive, the routine operation that can not spread to hospital and R＆D institution uses；
Second method is the method using piezoelectric supersonic microneedle " punching ", and this method is under microscopical visual angle
It is fixed first in the nine o'clock direction of egg mother cell using sticking needle, later using operation needle under piezoelectric ceramic ultrasonic vibration,
Egg mother cell can be punched using ultrasound, then substance in egg mother cell is extracted with micropin again, this method requirement
Scientific research personnel need to have more rich operating experience, and due to ultrasonic this feature of longitudinal conductibility, during the experiment, very
It is easy, since ultrasonic energy is excessive, to damage structure in egg mother cell.
Therefore, in view of the above technical problems, it is necessary to which a kind of piezoelectric supersonic cutting system of egg mother cell oolemma is provided
And method.
Invention content
In view of this, the purpose of the present invention is to provide a kind of piezoelectric supersonic cutting system of egg mother cell oolemma and sides
Method.
To achieve the goals above, the technical solution that one embodiment of the invention provides is as follows：
A kind of piezoelectric supersonic cutting system of egg mother cell oolemma, the piezoelectric supersonic cutting system include：
Egg mother cell pose adjusts chip, for carrying out pose adjusting and bottom fixation sticking to egg mother cell；
Egg mother cell holding device, including elbow sticking micropin and the micropin clamper for elbow sticking micropin to be clamped,
The end of the elbow sticking micropin is unilateral concave circular arc sticking syringe needle, and elbow sticking micropin is internally provided with a bayonet,
End is equipped with a notch；
Egg mother cell ultrasonic cutting scalpel, the egg mother cell ultrasonic cutting scalpel are used for from gap position to ovum
Mother cell oolemma carries out feed cutting.
As a further improvement on the present invention, the notch of the elbow sticking micropin end using grinding and toasts passivation shape
At.
As a further improvement on the present invention, the egg mother cell holding device is equipped with connecting hole, the egg mother cell
Connecting hole on holding device is connected with the first vacuum pump, and the sticking pressure by controlling first vacuum pump is realized female to ovum thin
The control of born of the same parents' oolemma sticking amount.
As a further improvement on the present invention, the egg mother cell pose adjusts chip and includes basal layer, is located at basal layer
On PDMS layer and electrode layer positioned at PDMS layer, the center of the electrode layer be equipped with for carrying out vacuum suction to egg mother cell
The operating area held, the operating area carry out vacuum hold by the second vacuum pump to egg mother cell.
As a further improvement on the present invention, the electrode layer includes the screw electrode of several array distributions, the spiral
Electrode includes the electrode that electrode pad is connected one by one with electrode pad, and the electrode self-electrode weld pad is set to operating area spiral
It sets.
As a further improvement on the present invention, the egg mother cell ultrasonic cutting scalpel includes the fixation set gradually
Bar, piezoelectric ceramics package module, luffing joint element for bar, amplitude transformer, cutter head connector, cutter head.
As a further improvement on the present invention, the cutter head includes horizontal segment and tilting section, and the horizontal segment is used for ovum
Mother cell oolemma is cut, and tilting section is for connecting horizontal segment and cutter head connector.
The technical solution that another embodiment of the present invention provides is as follows：
A kind of piezoelectric supersonic cutting process of egg mother cell oolemma, the piezoelectric supersonic cutting process include：
S1, egg mother cell is positioned over to the enterprising line position appearance adjusting of egg mother cell pose adjusting chip, and to egg mother cell bottom
Sticking is fixed in portion；
S2, determine that egg mother cell targets operating area, it is saturating to egg mother cell target area by egg mother cell holding device
Oolemma carries out sticking；
S3, using egg mother cell ultrasonic cutting scalpel along the gap position of egg mother cell holding device to egg mother cell
Oolemma carries out pulsed cutting, detaches oolemma sticking part and egg mother cell main part.
As a further improvement on the present invention, the notch of the elbow sticking micropin end using grinding and toasts passivation shape
At.
As a further improvement on the present invention, the step S2 further includes：
Sticking pressure by controlling vacuum pump realizes the control to egg mother cell oolemma sticking amount.
As a further improvement on the present invention, the step S1 further includes：
Adjust that screw electrode access amplitude, frequency on chip are identical to egg mother cell pose, 90 ° of carrier phase shift it is humorous
Wave signal, egg mother cell move after by traveling wave dielectrophoretic force toward screw electrode center.
As a further improvement on the present invention, the elbow sticking micropin and the ovum of egg mother cell holding device end
90 ° of angle between the cutter head of mother cell ultrasonic cutting scalpel end.
The beneficial effects of the invention are as follows：
The present invention devises a kind of piezoelectric supersonic cutting system and method more efficiently, safe, can meet ovum mother
The micro- cutting requirement of cell, while the damage to egg mother cell is reduced to the greatest extent；
The piezoelectric supersonic cutting system of egg mother cell oolemma can be avoided effectively since the longitudinal conductibility of ultrasonic energy is to ovum mother
This disadvantage of cellular damage, egg mother cell ultrasonic cutting scalpel cut oolemma from the tangential direction on egg mother cell surface layer
It cuts, while the system is equipped with special egg mother cell holding device and bottom egg mother cell pose adjusts chip, it can be female to ovum thin
The Auxiliary support that born of the same parents play the role of can reduce the forced deformation of egg mother cell during the cutting process, and bite can more be concentrated effectively
It is direct target area is cut, to reduce damage to egg mother cell.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments described in invention, for those of ordinary skill in the art, without creative efforts,
Other drawings may also be obtained based on these drawings.
Fig. 1 is the dimensional structure diagram of piezoelectric supersonic cutting system in first embodiment of the invention；
Fig. 2 a are the overlooking structure diagram of piezoelectric supersonic cutting system in first embodiment of the invention；
Fig. 2 b are the partial enlarged view of circled in Fig. 2 a；
Fig. 3 is the structural schematic diagram of egg mother cell ultrasonic cutting scalpel in first embodiment of the invention；
Fig. 4 is the structural schematic diagram of egg mother cell holding device in first embodiment of the invention；
Fig. 5 a are the schematic side view that egg mother cell oolemma target area is cut in first embodiment of the invention；
Fig. 5 b are the local overlooking schematic diagram that egg mother cell oolemma target area is cut in first embodiment of the invention；
Fig. 6 is the stereoscopic schematic diagram that egg mother cell oolemma target area is cut in first embodiment of the invention；
Fig. 7 is the structural schematic diagram of elbow sticking micropin in first embodiment of the invention；
Fig. 8 is the flow diagram of piezoelectric supersonic cutting process in second embodiment of the invention.
Specific implementation mode
In order to make those skilled in the art more fully understand the technical solution in the present invention, below in conjunction with of the invention real
The attached drawing in example is applied, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
The every other embodiment that technical staff is obtained without making creative work, should all belong to protection of the present invention
Range.
The invention discloses a kind of piezoelectric supersonic cutting systems of egg mother cell oolemma, including：
Egg mother cell pose adjusts chip, for carrying out pose adjusting and bottom fixation sticking to egg mother cell；
Egg mother cell holding device, including elbow sticking micropin and the micropin clamper for elbow sticking micropin to be clamped,
The end of elbow sticking micropin is unilateral concave circular arc sticking syringe needle, and elbow sticking micropin is internally provided with a bayonet, end
Equipped with a notch；
Egg mother cell ultrasonic cutting scalpel, egg mother cell ultrasonic cutting scalpel are used for from gap position female to ovum thin
Born of the same parents' oolemma carries out feed cutting.
The invention also discloses a kind of piezoelectric supersonic cutting process of egg mother cell oolemma, including：
S1, egg mother cell is positioned over to the enterprising line position appearance adjusting of egg mother cell pose adjusting chip, and to egg mother cell bottom
Sticking is fixed in portion；
S2, determine that egg mother cell targets operating area, it is saturating to egg mother cell target area by egg mother cell holding device
Oolemma carries out sticking；
S3, using egg mother cell ultrasonic cutting scalpel along the gap position of egg mother cell holding device to egg mother cell
Oolemma carries out pulsed cutting, detaches oolemma sticking part and egg mother cell main part.
The present invention can meet the experiment demand of many cell manipulations in current manual's supplementary reproduction field, such as：Oolemma is thin
Change, PGD (gene diagnosis before transplanting), PGS technologies (science of heredity screening before Embryonic limb bud cell) etc..
Meanwhile the present invention uses the cell pose designed based on screw electrode and vacuum absorption device to adjust chip, therefore
It can be so that cell stops at an arbitrary position.And by way of the vacuum absorption holes for adjusting chip sticking needle and pose are combined,
Cell is fixed in such a way that two support arms are combined, more firm effect can be played.
Meanwhile the negative pressure that sticking needle is generated by high-accuracy vacuum pump, it can reach accurate fixed to the sticking amount of oolemma
Amount control.
In addition, can more efficient, safe, accurately be targeted to cell oolemma for the micro- cutting apparatus of cooperation cell ultrasound
Region is cut off, and the present invention specially devises a kind of using " drawing concave round end micropin+card grinding instrument cutting " method thus
Manufactured abnormity asymmetric cutting auxiliary sticking needle.The advantage of the design is：The inner needle wall design of concave can be effective
It avoids causing oolemma sticking amount error to the interference that cell oolemma sticking amount generates due to micropin capillary force；Meanwhile by
After syringe needle unilateral side is cut off by card grinding instrument, ultrasonic cutting knife is more favorable for oolemma region to be cut " feed ", without making
It is in contact with micropin syringe needle at the micro- bite of ultrasound and generates mechanical collision and abrasion.
Finally, pass through the core component of the system --- egg mother cell ultrasonic cutting scalpel, in the high frequency of piezoelectric ceramics
It under vibration, is cut along the tangential direction of cell oolemma, can accomplish more targetedly target area cutting experiment.
The specific embodiment of the invention is described in detail below in conjunction with Figure of description.
Join Fig. 1, Fig. 2 a, Fig. 2 b, shown in Fig. 4, Fig. 7, the piezoelectricity of the egg mother cell oolemma in first embodiment of the invention
Ultrasonic cutting system includes：
Egg mother cell pose adjusts chip 10, for carrying out pose adjusting and bottom fixation sticking to egg mother cell 40；
Egg mother cell holding device 20, including elbow sticking micropin 21 and the micropin for elbow sticking micropin to be clamped are clamped
The end of device 22, elbow sticking micropin is unilateral concave circular arc sticking syringe needle, and elbow sticking micropin is internally provided with a bayonet
211, end is equipped with a notch 212；
Egg mother cell ultrasonic cutting scalpel 30, egg mother cell ultrasonic cutting scalpel 30 are used for from 212 position of notch
Feed cutting is carried out to egg mother cell oolemma.
Piezoelectric supersonic cutting system in the present embodiment includes that egg mother cell pose adjusts chip 10, egg mother cell sticking dress
20,30 three parts of egg mother cell ultrasonic cutting scalpel are set, each section is described in detail below.
Egg mother cell ultrasonic cutting scalpel：
Join shown in Fig. 3, Fig. 6, egg mother cell ultrasonic cutting scalpel 30 includes the fixed link 31 set gradually, piezoelectric ceramics
Package module 32, luffing joint element for bar 33, amplitude transformer 34, cutter head connector 35, cutter head 36.
Egg mother cell ultrasonic cutting scalpel 30 is in carrying out egg mother cell oolemma cutting process, for the ease of studying people
Member can preferably observe the working condition of cutter head 36 and egg mother cell 40 under the microscope, and cutter head 36 uses two-piece design, point
For horizontal segment 361 and tilting section 362.Horizontal segment 361 is mainly used for the cutting to oolemma, and 362 dimensional structure of tilting section is macro
Size is seen, for the fixation with cutter head connector 35.Cutter head 36 is integrally formed under MEMS technology by silicon chip, in egg mother cell
Will not generate toxicity to egg mother cell in cutting process, and can guarantee under microscopic dimensions and ultrasonic vibration certain intensity without
It deforms.
Since egg mother cell is by long-time, continuous ultrasound wave impact, it is easy to cause egg mother cell disintegration, inactivation.Therefore, originally
Invention inputs piezoelectric ceramics package module 32 using pulsed, discontinuous signal so that cutter head 36 uses pulse to oolemma
Formula cutting process.This method can in the case where not reducing stock-removing efficiency, improve egg mother cell test survival rate, reduce experiment at
This.
The major function of egg mother cell ultrasonic cutting scalpel 30：Power supply and control are accessed in piezoelectric ceramics package module 32
Under the premise of signal, piezoelectric ceramics generate high frequency axial vibration, by the output shaft of piezoelectric ceramics package module 32 pass to
The luffing joint element for bar 33 of cooperation finally transfer its energy to cutter head 36 to be indirectly transferred to amplitude transformer 34.Cutter head 36 obtains
After obtaining ultrasonic vibration energy, can targeting excision or thinning more efficiently be carried out to oolemma, without making egg mother cell stress
Flexible deformation occurs, influences cutting effect.
Egg mother cell pose adjusts chip：
Join Fig. 1, Fig. 2 a, Fig. 2 b, Fig. 5 a, Fig. 5 b, shown in Fig. 6, egg mother cell pose adjust chip 10 include basal layer 11,
PDMS layer 12 (polydimethylsiloxane, dimethyl silicone polymer) on basal layer and on PDMS layer
Electrode layer 13, the center of electrode layer 13 are equipped with the operating area for carrying out vacuum hold to egg mother cell, and operating area passes through
Vacuum pump carries out vacuum hold to egg mother cell.Wherein, electrode layer includes the screw electrode of several array distributions, screw electrode packet
The electrode 132 that electrode pad 131 is connected one by one with electrode pad is included, 132 self-electrode weld pad 131 of electrode is set to operating area spiral
It sets.
The major function that egg mother cell pose adjusts chip is：Chip is located at the bottom of egg mother cell, is egg mother cell
" pedestal " of micromanipulation.When egg mother cell ultrasonic cutting scalpel 30 carries out oolemma cutting to egg mother cell target area
Before, the pose of egg mother cell is adjusted in the dielectrophoretic force which is generated by screw electrode.Wait for that egg mother cell is adjusted to
After suitable position, the aperture of chip center is connected with vacuum pump (not shown), and negative pressure is generated to realize to quilt by vacuumizing
Operate the fixation RETENTION BY SOILS of egg mother cell.
In conclusion the effect of egg mother cell pose adjusting chip in the present invention is：In egg mother cell ultrasonic cutting hand
Art knife 30 carries out in cutting process egg mother cell target area, provides pose adjusting and Auxiliary support effect, prevents in ovum
" disengaging " occurs in mother cell oolemma cutting process.
Egg mother cell pose adjusts chip one and is divided into three layers, respectively：Electrode layer, PDMS layer, basal layer.Electrode layer by
Ito glass carries out photoetching under etch mask version and forms, and shown in ginseng Fig. 2 b, screw electrode is using spiral center as origin, rotation angle
90 ° of progress circumference array of degree.When work, once logical amplitude, frequency are identical for four screw electrodes, the electric signal that 90 ° of phase angle difference.
Therefore, when the egg mother cell being opposite on chip carries out in operating process, if when the egg mother cell not heart in the electrodes, ovum is female thin
Born of the same parents are moved by traveling wave dielectrophoretic force toward electrode centers, and after egg mother cell is moved to electrode centers, egg mother cell is situated between by electricity rotation
Electrophoretic force starts to rotate with electrode centers uniform speed slow.When egg mother cell rotation arrival ultrasonic cutting is specified requires position, stop
Only to screw electrode input electrical signal.Meanwhile vacuum pump connects the aperture at screw electrode center and generates negative pressure, to egg mother cell into
Row sticking is fixed.After the completion of waiting for the above operation, egg mother cell is fixed by chip in place, carries out next step sticking later
The fixation of needle target area and the target area cutting of ultrasonic cutting knife.
Egg mother cell holding device：
Join Fig. 4, Fig. 5 a, Fig. 5 b, shown in Fig. 6, Fig. 7, egg mother cell holding device 20 includes elbow sticking micropin 21 and uses
In the micropin clamper 22 of clamping elbow sticking micropin, the end of elbow sticking micropin is unilateral concave circular arc sticking syringe needle,
Elbow sticking micropin is internally provided with a bayonet 211, and end is equipped with a notch 212.
The function in the present invention of egg mother cell holding device 20 and effect are：After egg mother cell after pose is adjusted,
Egg mother cell oolemma is cut in next step.Since egg mother cell is only fixed in bottom by sticking, so as to cause
In egg mother cell cutting process, cutter head carry out egg mother cell surface layer oolemma tangential cutting when, egg mother cell still can be easy by
" forced deformation " occurs for power so that cutter head can not be completed to cut from surface layer " slipping over ".For this problem, the present invention is innovative
Ground, which is proposed, fixes egg mother cell target area progress sticking using second auxiliary support arm (i.e. elbow sticking micropin 21).
As shown in Fig. 5 a, 5b, after egg mother cell is by 2 Auxiliary supports, then egg mother cell cutting is carried out, it can more efficient, essence
Standard targetedly cuts target area.
In addition to this, what the present invention also innovated proposes using the indirect realization of control to ultraprecise vacuum pump to elbow suction
The control of 21 negative pressure of vacuum of micropin is held, oolemma surface layer sticking amount is accurately controlled to realize.Really realize oolemma
Quantitative accurate is cut.Specifically, egg mother cell holding device is equipped with connecting hole (not shown), on egg mother cell holding device
Connecting hole is connected with vacuum pump, and the sticking by controlling the vacuum pump presses the control realized to egg mother cell oolemma sticking amount.
Join shown in Fig. 5 a, 5b, in order to reduce suction of the elbow sticking micropin 21 to the damage raising of egg mother cell to oolemma
Holding effect fruit and efficiency, while egg mother cell oolemma is cut for preferably cooperation ultrasonic cutting knife, the present invention is innovative
Devise a kind of unilateral grinding concave circular arc sticking syringe needle of abnormity.
Join shown in Fig. 7, the inner end diameter of the elbow sticking micropin 21 is first big, and-rear small-what is become larger again is designed with conducive to transparent
Band, which after sticking, is reached bayonet 211, preferably oolemma to be fixed, and next step cutting operation is convenient for.Simultaneously as
Its most of internal diameter is major diameter, is only minor diameter with oolemma contact portion, which can effectively avoid due to micropin
Sticking amount error caused by the interference that capillary force generates egg mother cell oolemma sticking amount, convenient for the suction of accurate transparent ribbon amount
It holds.In addition, being using the purpose of the ground design of micropin unilateral side inner wall：After sticking needle adsorbs oolemma, egg mother cell is super
Sound cutting scalpel can efficiently use the notch 212, to oolemma region to be cut " feed ", without causing egg mother cell super
Sound cutting scalpel 30 is in contact with elbow sticking micropin 21 and generates mechanical collision and abrasion.Finally, which adopts
With wheel grinding and use alcolhol burner baking passivation for the design of arc-shaped, it is intended to be avoided in egg mother cell holding course sharp
Corner angle produce egg mother cell surface layer and damage.
Join shown in Fig. 8, the piezoelectric supersonic cutting process of egg mother cell oolemma in second embodiment of the invention, including：
S1, egg mother cell is positioned over to the enterprising line position appearance adjusting of egg mother cell pose adjusting chip, and to egg mother cell bottom
Sticking is fixed in portion；
S2, determine that egg mother cell targets operating area, it is saturating to egg mother cell target area by egg mother cell holding device
Oolemma carries out sticking；
S3, using egg mother cell ultrasonic cutting scalpel along the gap position of egg mother cell holding device to egg mother cell
Oolemma carries out pulsed cutting, detaches oolemma sticking part and egg mother cell main part.
Operation overall procedure in the present embodiment is specific as follows：
1. egg mother cell to be operated is placed on egg mother cell pose and adjusts chip by related preparation before carrying out experiment
On.
2. the screw electrode access amplitude, frequency on pair egg mother cell pose adjusting chip are identical, 90 ° of carrier phase shift
Harmonic signal.
3. assuming egg mother cell not at screw electrode center, egg mother cell is after by traveling wave dielectrophoretic force, toward screw electrode
It moves at center.After egg mother cell drives to screw electrode center, egg mother cell is by electricity rotation dielectrophoretic force with egg mother cell
Center starts uniform rotation.Microscopically observation stops the defeated of electric signal after egg mother cell turns to intended operating location
Enter, while the vacuum pump that access screw electrode centre bore in chip is adjusted to egg mother cell pose is powered, its centre bore is made to start
It vacuumizes, is fixed to carry out sticking to egg mother cell bottom.
4. join Fig. 1, Fig. 2 a, Fig. 2 b, Fig. 5 a, Fig. 5 b, shown in Fig. 6, Fig. 7, by elbow sticking micropin close to egg mother cell
Oolemma targets cutting zone, true to the connecting hole access ultraprecise of micropin clamper after sticking micropin is close to target area
Sky pump.The control realized to egg mother cell oolemma sticking amount is accurately controlled by the sticking pressure to vacuum pump.
5. joining shown in Fig. 5, Fig. 6, after the completion of waiting for aforesaid operations, scalpel is cut along ovum using egg mother cell piezoelectric supersonic
" pulsed " cutting is carried out to oolemma target area at mother cell sticking micropin syringe needle gap position so that sticking is for transparent
Band sticking part and egg mother cell main body detach, to realize quantitative cutting and the oolemma thinning function to oolemma.
Compared with prior art, the invention has the advantages that：
1. using Special vacuum absorption type egg mother cell holding device as the positioning of egg mother cell oolemma and crawl dress
It sets, while by realizing that accurate adjust presses size to the sticking of oolemma to the control of ultraprecise vacuum pump, to realize indirectly
Adjust the size of oolemma cutting quantity；
2. for cooperation egg mother cell ultrasonic cutting scalpel work, the micropin shape of egg mother cell holding device is carried out
Optimization design, the method by drawing the additional card grinding instrument of " concave " syringe needle that needle instrument is drawn out to carry out grinding, devises more
Add and carries out target area elbow sticking micropin that is accurate, quantitatively cutting conducive to bite " feed "；
Egg mother cell pose is adjusted into chip, egg mother cell holding device, egg mother cell ultrasound 3. innovatively proposing
The egg mother cell oolemma ultrasonic cutting method of three module compounding practices of scalpel is cut, there is presently no for single celled
Ultrasonic cutting technology；
4. using the egg mother cell oolemma pulsed cutting process of " feeding-ultrasonic cutting-feeding-ultrasonic cutting ", have
Effect solves the problems, such as that cell is impacted lower easy in inactivation by long-time ultrasonic continuous；
5. the cutter head and cell sticking micropin of egg mother cell ultrasonic cutting scalpel are all made of " elbow " design, convenient for aobvious
The real-time observation to cell manipulation state is realized under micro mirror or under machine vision, is easy to implement the automation of cell micromanipulation.
In conclusion the present invention utilizes organic cooperation of above-mentioned three parts, it can be more efficient, safely and conveniently right
Egg mother cell oolemma cut, thinning, has evaded the shortcoming of traditional egg mother cell oolemma micromanipulation.In addition,
Since egg mother cell pose adjusts chip and egg mother cell holding device technical know-how relative maturity, the present invention only need to
Upper module carries out the improvement and innovation of part, and is subject to three and works in coordination use, just can realize more excellent experiment effect.
Therefore, the present invention greatly reduces the R＆D costs and experimental cost of egg mother cell micromanipulative technique.On furthermore
Interchangeability is strong, flexible between stating three parts, in carrying out micromanipulation for the egg mother cell of different type, size, energy
Adjustment and optimization slightly is carried out, without changing overall structure and experiment effect, to fundamentally reduce to experiment
Personnel contact the cost of new equipment, the skill requirement of new method and experimental facilities production and processing again, have wide scientific research
Academic research foreground and clinical medicine, human assistance field of reproduction scientific research value and marketing meaning.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiment being appreciated that.
Claims (12)
1. a kind of piezoelectric supersonic cutting system of egg mother cell oolemma, which is characterized in that the piezoelectric supersonic cutting system packet
It includes：
Egg mother cell pose adjusts chip, for carrying out pose adjusting and bottom fixation sticking to egg mother cell；
Egg mother cell holding device, including elbow sticking micropin and the micropin clamper for elbow sticking micropin to be clamped, it is described
The end of elbow sticking micropin is unilateral concave circular arc sticking syringe needle, and elbow sticking micropin is internally provided with a bayonet, end
Equipped with a notch；
Egg mother cell ultrasonic cutting scalpel, the egg mother cell ultrasonic cutting scalpel are used for from gap position female to ovum thin
Born of the same parents' oolemma carries out feed cutting.
2. the piezoelectric supersonic cutting system of egg mother cell oolemma according to claim 1, which is characterized in that the elbow
The notch of sticking micropin end is using grinding and toasts passivation formation.
3. the piezoelectric supersonic cutting system of egg mother cell oolemma according to claim 1, which is characterized in that the ovum is female
Cell holding device is equipped with connecting hole, and the connecting hole on the egg mother cell holding device is connected with the first vacuum pump, passes through
Control control of the sticking pressure realization of first vacuum pump to egg mother cell oolemma sticking amount.
4. the piezoelectric supersonic cutting system of egg mother cell oolemma according to claim 1, which is characterized in that the ovum is female
It includes basal layer, the PDMS layer on basal layer and the electrode layer positioned at PDMS layer, the electrode that cell pose, which adjusts chip,
The center of layer is equipped with the operating area for carrying out vacuum hold to egg mother cell, and the operating area passes through the second vacuum pump pair
Egg mother cell carries out vacuum hold.
5. the piezoelectric supersonic cutting system of egg mother cell oolemma according to claim 4, which is characterized in that the electrode
Layer includes the screw electrode of several array distributions, and the screw electrode includes the electricity that electrode pad is connected one by one with electrode pad
Pole, the electrode self-electrode weld pad are arranged to operating area spiral.
6. the piezoelectric supersonic cutting system of egg mother cell oolemma according to claim 1, which is characterized in that the ovum is female
Cell ultrasonic cutting scalpel include the fixed link set gradually, piezoelectric ceramics package module, luffing joint element for bar, amplitude transformer,
Cutter head connector, cutter head.
7. the piezoelectric supersonic cutting system of egg mother cell oolemma according to claim 6, which is characterized in that the cutter head
Including horizontal segment and tilting section, the horizontal segment is for cutting egg mother cell oolemma, and tilting section is for connecting level
Section and cutter head connector.
8. a kind of piezoelectric supersonic cutting process of egg mother cell oolemma, which is characterized in that the piezoelectric supersonic cutting process packet
It includes：
S1, egg mother cell be positioned over to egg mother cell pose adjust the enterprising line position appearance of chip and adjust, and to egg mother cell bottom into
The fixed sticking of row；
S2, determine that egg mother cell targets operating area, by egg mother cell holding device to egg mother cell target area oolemma
Carry out sticking；
It is S3, transparent to egg mother cell along the gap position of egg mother cell holding device using egg mother cell ultrasonic cutting scalpel
Band carries out pulsed cutting, detaches oolemma sticking part and egg mother cell main part.
9. the piezoelectric supersonic cutting process of egg mother cell oolemma according to claim 8, which is characterized in that the elbow
The notch of sticking micropin end is using grinding and toasts passivation formation.
10. the piezoelectric supersonic cutting process of egg mother cell oolemma according to claim 8, which is characterized in that the step
Suddenly S2 further includes：
Sticking pressure by controlling vacuum pump realizes the control to egg mother cell oolemma sticking amount.
11. the piezoelectric supersonic cutting process of egg mother cell oolemma according to claim 8, which is characterized in that the step
Suddenly S1 further includes：
Screw electrode on chip is adjusted to egg mother cell pose and accesses the harmonic wave letter that amplitude, frequency are identical, and 90 ° of carrier phase shift
Number, egg mother cell moves after by traveling wave dielectrophoretic force toward screw electrode center.
12. the piezoelectric supersonic cutting process of egg mother cell oolemma according to claim 8, which is characterized in that the ovum
Between the elbow sticking micropin of mother cell holding device end and the cutter head of the egg mother cell ultrasonic cutting scalpel end
90 ° of angle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810455757.8A CN108456641B (en) | 2018-05-14 | 2018-05-14 | Piezoelectric ultrasonic cutting system and method for oocyte zona pellucida |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810455757.8A CN108456641B (en) | 2018-05-14 | 2018-05-14 | Piezoelectric ultrasonic cutting system and method for oocyte zona pellucida |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108456641A true CN108456641A (en) | 2018-08-28 |
| CN108456641B CN108456641B (en) | 2023-08-15 |
Family
ID=63214745
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810455757.8A Active CN108456641B (en) | 2018-05-14 | 2018-05-14 | Piezoelectric ultrasonic cutting system and method for oocyte zona pellucida |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108456641B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111229576A (en) * | 2020-01-19 | 2020-06-05 | 苏州大学 | Biological micro-cutting device based on flexible vibration reduction ultrasonic amplitude transformer |
| CN111849768A (en) * | 2020-07-14 | 2020-10-30 | 苏州大学 | Egg cell multi-efficiency accurate electrical stimulation device and method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004113391A (en) * | 2002-09-25 | 2004-04-15 | Olympus Corp | Ultrasonograph |
| CN101319972A (en) * | 2008-04-21 | 2008-12-10 | 浙江大学 | Servo ultrasonic vibration micro-dissection device based on vision |
| CN102268360A (en) * | 2011-04-27 | 2011-12-07 | 南开大学 | Supersonic vibration microscopic cell penetrating system based on piezoelectric ceramic driving |
| US20160175001A1 (en) * | 2014-12-19 | 2016-06-23 | Ethicon Endo-Surgery, Llc | Ultrasonic surgical instrument with dual modes |
| US20170166865A1 (en) * | 2014-07-06 | 2017-06-15 | Jianjun Peng | Electric ovum denuding device and ovum denuding method |
| CN208430178U (en) * | 2018-05-14 | 2019-01-25 | 苏州大学 | The piezoelectric supersonic cutting system of egg mother cell oolemma |
-
2018
- 2018-05-14 CN CN201810455757.8A patent/CN108456641B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004113391A (en) * | 2002-09-25 | 2004-04-15 | Olympus Corp | Ultrasonograph |
| CN101319972A (en) * | 2008-04-21 | 2008-12-10 | 浙江大学 | Servo ultrasonic vibration micro-dissection device based on vision |
| CN102268360A (en) * | 2011-04-27 | 2011-12-07 | 南开大学 | Supersonic vibration microscopic cell penetrating system based on piezoelectric ceramic driving |
| US20170166865A1 (en) * | 2014-07-06 | 2017-06-15 | Jianjun Peng | Electric ovum denuding device and ovum denuding method |
| US20160175001A1 (en) * | 2014-12-19 | 2016-06-23 | Ethicon Endo-Surgery, Llc | Ultrasonic surgical instrument with dual modes |
| CN208430178U (en) * | 2018-05-14 | 2019-01-25 | 苏州大学 | The piezoelectric supersonic cutting system of egg mother cell oolemma |
Non-Patent Citations (2)
| Title |
|---|
| BIN WANG ET.AL.: "The in vitro and in vivo development of goat embryos produced by intracytoplasmic sperm injection using tail-cut spermatozoa", ZYGOTE * |
| SOICHI NAKAMURA ET.AL.: "Mechanical Properties of The Cell Surface in Starfish eggs", DEVELOP. GROWTH AND DIFFER * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111229576A (en) * | 2020-01-19 | 2020-06-05 | 苏州大学 | Biological micro-cutting device based on flexible vibration reduction ultrasonic amplitude transformer |
| CN111849768A (en) * | 2020-07-14 | 2020-10-30 | 苏州大学 | Egg cell multi-efficiency accurate electrical stimulation device and method |
| CN111849768B (en) * | 2020-07-14 | 2022-07-19 | 苏州大学 | Egg cell multi-efficiency accurate electrical stimulation device and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108456641B (en) | 2023-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110367977B (en) | Photoelectric integrated stretchable flexible nerve electrode and preparation method thereof | |
| CN113195114B (en) | Ultrasonic device for use with synthetic cavitation nuclei | |
| CN208430178U (en) | The piezoelectric supersonic cutting system of egg mother cell oolemma | |
| CN108456641A (en) | The piezoelectric supersonic cutting system and method for egg mother cell oolemma | |
| CN105943031B (en) | Wearable TCD,transcranial Doppler nerve stimulation and electrophysiological recording association system and method | |
| CN104178422B (en) | A kind of neural axon tractive grower | |
| CN104974935A (en) | device with annular micro-channel chip for cell culture | |
| CN101342404A (en) | Method for manufacturing different-plane metal hollow fine needle for transdermal drug administration | |
| CN107245431A (en) | A kind of microinjection device precisely injected for cell drug and its operating method | |
| CN105344023A (en) | Device for generating vector noninvasive focusing electrical stimulation in combination with magnetic field and ultrasonic | |
| CN207785674U (en) | Multi-path laser acupuncture and moxibustion instrument | |
| WO2023019431A1 (en) | Device and method for stimulating secretion of cellular exosomes, and obtained exosomes and application thereof | |
| CN106725335B (en) | A clear all-in-one of creating of high accuracy fiber laser diagnosis for burn diagnosis and treatment | |
| CN108660072A (en) | Egg mother cell micromanipulation system and method based on target area Auxiliary support | |
| CN104971406A (en) | Medical needle with micro-reflector structure on surface and preparation method thereof | |
| CN104587567B (en) | A kind of preparation method of micro hollow silicon needle | |
| WO2023186053A1 (en) | Flexible neural electrode composite structure and manufacturing and implantation method therefor, and auxiliary implantation assembly | |
| CN205251643U (en) | Puncture guiding device in supersound guide plane | |
| CN203341819U (en) | Magnetic B-ultrasonic probe puncture device | |
| US11738185B2 (en) | In-plane metal microneedle array and manufacturing method therefor | |
| Gao et al. | Magnetically Manipulated Optoelectronic Hybrid Microrobots for Optically Targeted Non‐Genetic Neuromodulation | |
| CN204951839U (en) | Surface has needle for medical science of little reflector structure | |
| CN203694435U (en) | Ultrasonic transmission device for interventional treatment | |
| Gao et al. | Design and Simulation Optimization of a Novel Oocyte Ultrasonic Micro-dissection Instrument | |
| CN214018907U (en) | Roller type silicon-based accurate microneedle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |