CN108452240A - A kind of antitumor Chinese medicine composition and its application - Google Patents
A kind of antitumor Chinese medicine composition and its application Download PDFInfo
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Abstract
The present invention relates to a kind of antitumor Chinese medicine compositions and its application.The Chinese medicine composition is made of the bulk pharmaceutical chemicals of following parts by weight：9 15 parts of trigone, 9 15 parts of curcuma zedoary, 15 20 parts of the tuber of pinellia processed, 15 20 parts of Rhizoma Arisaematis (processed), 15 30 parts of Radix Astragali, 15 30 parts of Rhizoma Atractylodis Macrocephalae.The Chinese medicine composition of the present invention has dispel Yu Xiao Disorder, resolving phlegm and softening hard masses, righting strengthening the spleen and replenishing qi and other effects, animal experiments show that 45 sdenocarcinoma of stomach growth of transplanted human of nude mice MKN can be significantly inhibited, lower PCNA and EGFR expression in gastric cancer in nude mice tissue, change 256 ascites tumor rat model tumor microenvironments of walker, good therapeutic effect can be played to tumour, the Chinese medicine composition also has the advantages that flavour of a drug number is less, is easy to get convenient for preparation, abundant raw materials, is at low cost in addition.
Description
Technical field
The present invention relates to a kind of Chinese medicine compositions, specifically, being related to a kind of antitumor Chinese medicine composition and its application.
Background technology
Malignant tumour is also known as cancer, it has also become threatens common disease, the frequently-occurring disease of human life, is that China's urban population is dead
Ten serious diseases are because first of.According to the World Health Organization (WHO) report, the incidence of whole world cancer just with annual 3% speed increase,
Death toll is up to ten thousand people more than 600 due to cancer every year.Therefore, reinforce the research for the treatment of malignant tumor, improve existing various hands
The therapeutic effect of section, explores new therapy approach, improves the quality of life of cancer patient, reduces recurrence rate, reduces the death rate, is
The task of top priority of clinical tumor worker at present.
Modern medical therapy method mainly has operation excision, radiotherapy, chemotherapy, immunotherapy, targeted therapy etc.
Deng.Operative treatment is still that most of oncotherapies are preferred, but are only applicable to the tumor patient that clinical stages is early metaphase at present, right
The characteristics of tumor patient that late period has shifted can only use the palliative therapies means such as chemotherapy, radiotherapy, these treatments be
Normal tissue cell also has certain damage, toxicity severe patient patient may be caused to treat while killing tumor cell
It terminates；Immunization method is that oncotherapy opens a new way, but current curative effect and indication are still in groping.
Chinese medicine thinks that malignant tumour is to find expression in local systemic disease, its generation, development is one slow, progressive
Process, be organismic internal environment imbalance after topical manifestations.And think that the generation of tumour and phlegm, the stasis of blood are in close relations.Early in《The legendary god of farming
Book on Chinese herbal medicine warp》In, have the record of " phlegm knot stays drink phlegm addiction in the heart " etc, it is believed that " ordinary person upper, middle and lower have block person, be mostly phlegm ", again
Speech " carcinoma person, non-negative and positive healthy tendency are tied swollen, and being that the five internal organs hemostasis foul smell phlegm is stagnant forms ", " from gas at product, from product at phlegm, phlegm presss from both sides the stasis of blood
Blood, then at nest capsule ".Illustrate that tumour formation is for phlegm and blood stasis.For " phlegm-blood stasis " this cause of disease, the dissolving stasis reducing phlegm and resolving masses of use
Method is traditional one of big method of oncotherapy.But traditional Chinese medicine is more paid attention to transferring and carrying while eliminating local pathogenic factor
The anticancer function of high human body itself, building up healthful vital energy.Therefore, more than one thousand years carrys out Chinese medicine and there has been comparable understanding to tumour, except doing
More careful faces outside disease observation, also has accumulated many treatment prescriptions.Therefore, prescription essence is found and developed from Chinese Traditional Medicine
Refining, curative for effect, safely and effectively new Chinese medicine preparation has important clinic and scientific value for the prevention of tumour.
Chinese patent literature CN200810079458.5, publication date 2009.02.11, disclosing one kind having prevention and controls
Treat dual function treatment malignant tumour drug, the drug by Radix Astragali, Radix Codonopsis, honeysuckle, dandelion, radix bupleuri, radix scutellariae, in vain
Chinese herbaceous peony, acorus graminens soland, trigone, rhizoma arisaematis, the tuber of pinellia, oldenlandia diffusa, Radix Notoginseng mountain, beans root, curcuma zedoary drug composition, to each quasi-cancer cell
Growth have and inhibit and killing is gone out work(effect.Chinese patent literature CN201410430909.0, publication date 2014.11.19, it is public
A kind of Chinese medicine composition for treating malignant tumour is opened, made of the raw material of following weight parts：5-12 parts of root of Chinese trichosanthes, trigone 1-
3 parts, 1-3 parts of curcuma zedoary, 3-5 parts of peach kernel, 3-5 parts of safflower, 1-2 parts of Semen Strychni (processed), 1-3 parts of Eupolyphoge sinensis, 2-5 parts of scorpio, centipede 1-3
Part, 3-5 parts of the tuber of pinellia, 1-2 parts of rhizoma arisaematis, 3-5 parts of frankincense, 3-8 parts of bush, 5-8 parts of the root of bidentate achyranthes, 5-8 parts of hawthorn, 8-12 parts of Radix Codonopsis,
10-20 parts of Radix Astragali, which has the effect of inrigorating qi and promoting blood circulation, dissolving stasis removing toxic substances, reducing phlegm and resolving masses is subsided a swelling, for spleen deficiency and blood stasis phlegm
The treatment of turbid type malignant tumour has many advantages, such as that apparent curative effect, treating both manifestation and root cause of disease, toxic side effect are small.
However, theory of traditional Chinese medical science is of extensive knowledge and profound scholarship, Chinese medicinal formulae medicine compatibility method is numerous, and the compatibility relationship between medicinal material is even more micro-
It is wonderful, thus the cognition that Chinese medicine scholar can have oneself original prescription, and it is subject to compatibility again, to improve curative effect.In addition,
When Chinese medicinal formulae is applied to clinically, also to take into account it and prepare many factors such as difficulty or ease, cost of material.Therefore development good effect,
It is at low cost, convenient for preparation Chinese medicine preparation have broader market prospects.
Invention content
The purpose of the present invention is being directed to deficiency in the prior art, a kind of antitumor Chinese medicine composition is provided.
Another purpose of the present invention is to provide the purposes of the Chinese medicine composition.
Another purpose of the present invention is to provide the preparation method of the Chinese medicine composition.
To realize above-mentioned first purpose, the technical solution adopted by the present invention is that：
A kind of antitumor Chinese medicine composition, is made of the bulk pharmaceutical chemicals of following parts by weight：9-15 parts of trigone, curcuma zedoary 9-15
Part, tuber of pinellia 15-20 parts processed, 15-20 parts of Rhizoma Arisaematis (processed), 15-30 parts of Radix Astragali, 15-30 parts of Rhizoma Atractylodis Macrocephalae.
Preferably, it is made of the bulk pharmaceutical chemicals of following parts by weight：10-12 parts of trigone, 10-12 parts of curcuma zedoary, tuber of pinellia 16-18 processed
Part, 16-18 parts of Rhizoma Arisaematis (processed), 20-25 parts of Radix Astragali, 20-25 parts of Rhizoma Atractylodis Macrocephalae.
It is highly preferred that it is made of the bulk pharmaceutical chemicals of following parts by weight：10 parts of trigone, 10 parts of curcuma zedoary, 18 parts of the tuber of pinellia processed, day processed
18 parts of Southern Star, 20 parts of Radix Astragali, 20 parts of Rhizoma Atractylodis Macrocephalae.
As a kind of specific implementation mode of the present invention, the dosage form of the Chinese medicine composition is mixture, granule, capsule
Agent or tablet.
Preferably, the Chinese medicine composition preparation method includes the following steps：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 6- are added
The distilled water of 10 times of amounts, using steam distillation refluxing extraction 4-8 hour, collection volatile oil；By the Radix Astragali of pulverization process, system
Rhizoma arisaematis, the tuber of pinellia processed are added in extraction vessel, add the 6-10 times of distilled water measured of 3 taste medicinal material total weight, stir evenly, and decoct
It boils 2-3 times, each amount of water is 6-10 times of medicinal material total weight, 0.5-1.5 hours each, merges decoction liquor, is concentrated, and is done
It is dry, it weighs, obtains medicinal extract；
B) volatile oil clathrate compound is prepared：It takes and is equivalent to the inclusion essential oil material that 6-10 times of weight of oil of volatilization is measured, be added suitable
Distilled water is measured, saturated aqueous solution is configured at 40-60 DEG C, is stirred continuously down the absolute ethyl alcohol dilution for being slowly added to volatile oil
Liquid stirs 1-3 hour, is positioned over 2-8 DEG C of refrigerator 12-24 hour, takes out and filters, dry with 30-60 DEG C of petroleum ether precipitation
It is dry, it weighs, obtains volatile oil clathrate compound.
To realize above-mentioned second purpose, the technical solution adopted by the present invention is that：
As above any Chinese medicine composition application in preparation of anti-tumor drugs.
As the preference of the present invention, the tumour is gastric cancer, liver cancer, intestinal cancer, cancer of pancreas, gallbladder cancer or oesophagus
Cancer.
To realize above-mentioned third purpose, the technical solution adopted by the present invention is that：
As above the preparation method of any Chinese medicine composition, the preparation method include the following steps：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 6- are added
The distilled water of 10 times of amounts, using steam distillation refluxing extraction 4-8 hour, collection volatile oil；By the Radix Astragali of pulverization process, system
Rhizoma arisaematis, the tuber of pinellia processed are added in extraction vessel, add the 6-10 times of distilled water measured of 3 taste medicinal material total weight, stir evenly, and decoct
It boils 2-3 times, each amount of water is 6-10 times of medicinal material total weight, 0.5-1.5 hours each, merges decoction liquor, is concentrated, and is done
It is dry, it weighs, obtains medicinal extract；
B) volatile oil clathrate compound is prepared：It takes and is equivalent to the inclusion essential oil material that 6-10 times of weight of oil of volatilization is measured, be added suitable
Distilled water is measured, saturated aqueous solution is configured at 40-60 DEG C, is stirred continuously down the absolute ethyl alcohol dilution for being slowly added to volatile oil
Liquid stirs 1-3 hour, is positioned over 2-8 DEG C of refrigerator 12-24 hour, takes out and filters, dry with 30-60 DEG C of petroleum ether precipitation
It is dry, it weighs, obtains volatile oil clathrate compound.
Preferably, the inclusion essential oil material is selected from hydroxypropyl-β-cyclodextrin and/or beta-cyclodextrin.
Herein, the tuber of pinellia processed is the Preparation process product of the tuber of pinellia, preparation method：Take the net tuber of pinellia, size is separated, be soaked in water to
It is interior without the dry heart, take out；Another extracting liquorice is appropriate, adds water to cook secondary, and collecting decoction pours into the lime liquor made of suitable quantity of water,
It stirs evenly, the above-mentioned tuber of pinellia being impregnated with is added, impregnate, stir 1~2 time daily, and keep 12 or more immersion liquid pH value, until section yellow
Uniformly, mouth taste it is micro- take out when having numb feeling in the tongue, clean, dry in the shade or dry to get, wherein the often net tuber of pinellia of 100kg, with Radix Glycyrrhizae 15kg,
Quick lime 10kg.
The Rhizoma Arisaematis (processed) is the Preparation process product of rhizoma arisaematis, preparation method：Net rhizoma arisaematis is taken, is soaked in water respectively by size,
It changes water 2~3 times daily, when such as playing foam, adds alum (per 100kg rhizoma arisaematis, adding alum 2kg) after changing water, after steeping one, then into
Row changes water, until cutting opening is tasted and micro- taken out when having numb feeling in the tongue.Ginger slice, alum are set after adding suitable quantity of water to boil in pot, pours into Tian Nan
Star takes out when boiled altogether to without the dry heart, removes ginger splices, dries in the air to four to sixty percent dry, shaves, dry, wherein per 100kg rhizoma arisaematis, uses
Each 12.5kg of ginger, alum.
The invention has the advantages that：
1, the Chinese medicine composition mechanism of action of the invention is：Trigone, curcuma zedoary are monarch, trigone bitter, flat, it in the kind blood-breaking of work(
The strength of gas, blood-breaking is better than relieving stagnant Qi,《Kaibao Bencao》It carries " main Lao Pi Disorder lumps in the abdomen caking "；It is curcuma zedoary bitter, pungent, warm, disappear with dispersing blood stasis
The benefits of swollen,《Figure is through book on Chinese herbal medicine》It carries " it is the medicine most wanted that modern doctor, which controls all gas of accumulation, ".The tuber of pinellia processed, Rhizoma Arisaematis (processed) are minister, tuber of pinellia taste processed
It is pungent, warm, there is the effect of eliminating dampness and eliminating phlegm, dissolving lump and resolving mass, Rhizoma Arisaematis (processed) is bitterly, warm, has eliminating dampness and eliminating phlegm, the work(of dispersing swelling and dissipating binds
Effect.Radix Astragali, Rhizoma Atractylodis Macrocephalae, which are assistant, to be made, Radix Astragali slightly warm in nature sweet in flavor, return lung, the spleen channel, invigorating qi for strengthening superficies, torr sore myogenic；Rhizoma Atractylodis Macrocephalae bitter but sweet flavor temperature, returns
Spleen, stomach, air making-up and spleen enlivening, eliminate dampness and have diuretic effect,《Pearl sac》The benefits of carrying " dehumidifying QI invigorating and middle yang-tonifying ", playing QI invigorating righting altogether.More than
All medicines share has dispel Yu Xiao Disorder, resolving phlegm and softening hard masses, righting strengthening the spleen and replenishing qi and other effects.Animal experiments show that Chinese traditional medicine composition of the present invention
Object can significantly inhibit nude mice MKN-45 sdenocarcinoma of stomach growth of transplanted human, lower PCNA and EGFR expression in gastric cancer in nude mice tissue, change
Become walker-256 ascites tumor rat model tumor microenvironments, good therapeutic effect can be played to tumour.
2, Chinese medicine composition flavour of a drug number of the present invention is less, and convenient for preparing, abundant raw materials are easy to get, at low cost.
Specific implementation mode
It elaborates below to specific implementation mode provided by the invention.
The preparation (one) of 1 Chinese medicine composition of the present invention of embodiment
Each raw material medicines in portions by weight matches：9 parts of trigone, 9 parts of curcuma zedoary, 15 parts of the tuber of pinellia processed, 15 parts of Rhizoma Arisaematis (processed), Radix Astragali 15
Part, 15 parts of Rhizoma Atractylodis Macrocephalae.Preparation process is as follows：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 6 are added
The distilled water of times amount collected volatile oil using steam distillation refluxing extraction 8 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the distilled water of the 3 taste medicinal material total weight, 6 times of amounts, stir evenly, and decoct 3 times,
Each amount of water is 6 times of medicinal material total weight, 1.5 hours every time, merges decoction liquor, concentrates, dry, weighs, obtains medicinal extract；
B) volatile oil clathrate compound is prepared：Take the inclusion essential oil material hydroxy propyl-Beta-ring for being equivalent to volatilization 6 times of amounts of weight of oil
Appropriate distilled water is added in dextrin, and saturated aqueous solution is configured at 60 DEG C, is stirred continuously down the anhydrous second for being slowly added to volatile oil
Alcohol dilution stirs 1 hour, is positioned over 8 DEG C of refrigerators 12 hours, takes out and filters, is precipitated with 30 DEG C of petroleum ethers, dry, claims
Weight, obtains volatile oil clathrate compound；
C) in above-mentioned steps a) and b) obtained by medicinal extract, conventional pharmaceutical adjuvants are added in volatile oil clathrate compound, prepare plastic
Wafer, granule, tablet or mixture.
The preparation (two) of 2 Chinese medicine composition of the present invention of embodiment
Each raw material medicines in portions by weight matches：9 parts of trigone, 9 parts of curcuma zedoary, 20 parts of the tuber of pinellia processed, 20 parts of Rhizoma Arisaematis (processed), Radix Astragali 15
Part, 15 parts of Rhizoma Atractylodis Macrocephalae.Preparation process is as follows：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 10 are added
The distilled water of times amount collected volatile oil using steam distillation refluxing extraction 4 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the distilled water of the 3 taste medicinal material total weight, 10 times of amounts, stir evenly, and decoct 2 times,
Each amount of water is 10 times of medicinal material total weight, 0.5 hour every time, merges decoction liquor, concentrates, dry, weighs, is soaked
Cream；
B) volatile oil clathrate compound is prepared：The inclusion essential oil material beta-cyclodextrin for being equivalent to volatilization 10 times of amounts of weight of oil is taken,
Appropriate distilled water is added, saturated aqueous solution is configured at 40 DEG C, be stirred continuously down be slowly added to volatile oil absolute ethyl alcohol it is dilute
It releases liquid, stirs 3 hours, be positioned over 2 DEG C of refrigerators 12 hours, take out and filter, precipitated with 30 DEG C of petroleum ethers, it is dry, it weighs, obtains
To volatile oil clathrate compound；
C) in above-mentioned steps a) and b) obtained by medicinal extract, conventional pharmaceutical adjuvants are added in volatile oil clathrate compound, prepare plastic
Wafer, granule, tablet or mixture.
The preparation (three) of 3 Chinese medicine composition of the present invention of embodiment
Each raw material medicines in portions by weight matches：9 parts of trigone, 9 parts of curcuma zedoary, 15 parts of the tuber of pinellia processed, 15 parts of Rhizoma Arisaematis (processed), Radix Astragali 30
Part, 30 parts of Rhizoma Atractylodis Macrocephalae.Preparation process is as follows：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 8 are added
The distilled water of times amount collected volatile oil using steam distillation refluxing extraction 5 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the distilled water of the 3 taste medicinal material total weight, 8 times of amounts, stir evenly, and decoct 2 times,
Each amount of water is 8 times of medicinal material total weight, 1 hour every time, merges decoction liquor, concentrates, dry, weighs, obtains medicinal extract；
B) volatile oil clathrate compound is prepared：Take the inclusion essential oil material hydroxy propyl-Beta-ring for being equivalent to volatilization 8 times of amounts of weight of oil
Appropriate distilled water is added in dextrin and beta-cyclodextrin, and saturated aqueous solution is configured at 50 DEG C, is stirred continuously down and is slowly added to volatilize
The absolute ethyl alcohol dilution of oil stirs 2 hours, is positioned over 4 DEG C of refrigerators 18 hours, takes out and filters, heavy with 50 DEG C of petroleum ethers
It forms sediment, it is dry, it weighs, obtains volatile oil clathrate compound；
C) in above-mentioned steps a) and b) obtained by medicinal extract, conventional pharmaceutical adjuvants are added in volatile oil clathrate compound, prepare plastic
Wafer, granule, tablet or mixture.
The preparation (four) of 4 Chinese medicine composition of the present invention of embodiment
Each raw material medicines in portions by weight matches：9 parts of trigone, 9 parts of curcuma zedoary, 20 parts of the tuber of pinellia processed, 20 parts of Rhizoma Arisaematis (processed), Radix Astragali 30
Part, 30 parts of Rhizoma Atractylodis Macrocephalae.Preparation process is as follows：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 6 are added
The distilled water of times amount collected volatile oil using steam distillation refluxing extraction 8 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the distilled water of the 3 taste medicinal material total weight, 6 times of amounts, stir evenly, and decoct 3 times,
Each amount of water is 6 times of medicinal material total weight, 1.5 hours every time, merges decoction liquor, concentrates, dry, weighs, obtains medicinal extract；
B) volatile oil clathrate compound is prepared：Take the inclusion essential oil material hydroxy propyl-Beta-ring for being equivalent to volatilization 6 times of amounts of weight of oil
Appropriate distilled water is added in dextrin, and saturated aqueous solution is configured at 60 DEG C, is stirred continuously down the anhydrous second for being slowly added to volatile oil
Alcohol dilution stirs 1 hour, is positioned over 8 DEG C of refrigerators 12 hours, takes out and filters, is precipitated with 30 DEG C of petroleum ethers, dry, claims
Weight, obtains volatile oil clathrate compound；
C) in above-mentioned steps a) and b) obtained by medicinal extract, conventional pharmaceutical adjuvants are added in volatile oil clathrate compound, prepare plastic
Wafer, granule, tablet or mixture.
The preparation (five) of 5 Chinese medicine composition of the present invention of embodiment
Each raw material medicines in portions by weight matches：15 parts of trigone, 15 parts of curcuma zedoary, 15 parts of the tuber of pinellia processed, 15 parts of Rhizoma Arisaematis (processed), Radix Astragali 15
Part, 15 parts of Rhizoma Atractylodis Macrocephalae.Preparation process is as follows：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 10 are added
The distilled water of times amount collected volatile oil using steam distillation refluxing extraction 4 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the distilled water of the 3 taste medicinal material total weight, 10 times of amounts, stir evenly, and decoct 2 times,
Each amount of water is 10 times of medicinal material total weight, 0.5 hour every time, merges decoction liquor, concentrates, dry, weighs, is soaked
Cream；
B) volatile oil clathrate compound is prepared：The inclusion essential oil material beta-cyclodextrin for being equivalent to volatilization 10 times of amounts of weight of oil is taken,
Appropriate distilled water is added, saturated aqueous solution is configured at 40 DEG C, be stirred continuously down be slowly added to volatile oil absolute ethyl alcohol it is dilute
It releases liquid, stirs 3 hours, be positioned over 2 DEG C of refrigerators 12 hours, take out and filter, precipitated with 30 DEG C of petroleum ethers, it is dry, it weighs, obtains
To volatile oil clathrate compound；
C) in above-mentioned steps a) and b) obtained by medicinal extract, conventional pharmaceutical adjuvants are added in volatile oil clathrate compound, prepare plastic
Wafer, granule, tablet or mixture.
The preparation (six) of 6 Chinese medicine composition of the present invention of embodiment
Each raw material medicines in portions by weight matches：15 parts of trigone, 15 parts of curcuma zedoary, 20 parts of the tuber of pinellia processed, 20 parts of Rhizoma Arisaematis (processed), Radix Astragali 15
Part, 15 parts of Rhizoma Atractylodis Macrocephalae.Preparation process is as follows：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 8 are added
The distilled water of times amount collected volatile oil using steam distillation refluxing extraction 5 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the distilled water of the 3 taste medicinal material total weight, 8 times of amounts, stir evenly, and decoct 2 times,
Each amount of water is 8 times of medicinal material total weight, 1 hour every time, merges decoction liquor, concentrates, dry, weighs, obtains medicinal extract；
B) volatile oil clathrate compound is prepared：Take the inclusion essential oil material hydroxy propyl-Beta-ring for being equivalent to volatilization 8 times of amounts of weight of oil
Appropriate distilled water is added in dextrin and beta-cyclodextrin, and saturated aqueous solution is configured at 50 DEG C, is stirred continuously down and is slowly added to volatilize
The absolute ethyl alcohol dilution of oil stirs 2 hours, is positioned over 4 DEG C of refrigerators 18 hours, takes out and filters, heavy with 50 DEG C of petroleum ethers
It forms sediment, it is dry, it weighs, obtains volatile oil clathrate compound；
C) in above-mentioned steps a) and b) obtained by medicinal extract, conventional pharmaceutical adjuvants are added in volatile oil clathrate compound, prepare plastic
Wafer, granule, tablet or mixture.
The preparation (seven) of 7 Chinese medicine composition of the present invention of embodiment
Each raw material medicines in portions by weight matches：15 parts of trigone, 15 parts of curcuma zedoary, 20 parts of the tuber of pinellia processed, 20 parts of Rhizoma Arisaematis (processed), Radix Astragali 30
Part, 30 parts of Rhizoma Atractylodis Macrocephalae.Preparation process is as follows：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 6 are added
The distilled water of times amount collected volatile oil using steam distillation refluxing extraction 8 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the distilled water of the 3 taste medicinal material total weight, 6 times of amounts, stir evenly, and decoct 3 times,
Each amount of water is 6 times of medicinal material total weight, 1.5 hours every time, merges decoction liquor, concentrates, dry, weighs, obtains medicinal extract；
B) volatile oil clathrate compound is prepared：Take the inclusion essential oil material hydroxy propyl-Beta-ring for being equivalent to volatilization 6 times of amounts of weight of oil
Appropriate distilled water is added in dextrin, and saturated aqueous solution is configured at 60 DEG C, is stirred continuously down the anhydrous second for being slowly added to volatile oil
Alcohol dilution stirs 1 hour, is positioned over 8 DEG C of refrigerators 12 hours, takes out and filters, is precipitated with 30 DEG C of petroleum ethers, dry, claims
Weight, obtains volatile oil clathrate compound；
C) in above-mentioned steps a) and b) obtained by medicinal extract, conventional pharmaceutical adjuvants are added in volatile oil clathrate compound, prepare plastic
Wafer, granule, tablet or mixture.
The preparation (eight) of 8 Chinese medicine composition of the present invention of embodiment
Each raw material medicines in portions by weight matches：15 parts of trigone, 15 parts of curcuma zedoary, 15 parts of the tuber of pinellia processed, 15 parts of Rhizoma Arisaematis (processed), Radix Astragali 30
Part, 30 parts of Rhizoma Atractylodis Macrocephalae.Preparation process is as follows：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 10 are added
The distilled water of times amount collected volatile oil using steam distillation refluxing extraction 4 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the distilled water of the 3 taste medicinal material total weight, 10 times of amounts, stir evenly, and decoct 2 times,
Each amount of water is 10 times of medicinal material total weight, 0.5 hour every time, merges decoction liquor, concentrates, dry, weighs, is soaked
Cream；
B) volatile oil clathrate compound is prepared：The inclusion essential oil material beta-cyclodextrin for being equivalent to volatilization 10 times of amounts of weight of oil is taken,
Appropriate distilled water is added, saturated aqueous solution is configured at 40 DEG C, be stirred continuously down be slowly added to volatile oil absolute ethyl alcohol it is dilute
It releases liquid, stirs 3 hours, be positioned over 2 DEG C of refrigerators 12 hours, take out and filter, precipitated with 30 DEG C of petroleum ethers, it is dry, it weighs, obtains
To volatile oil clathrate compound；
C) in above-mentioned steps a) and b) obtained by medicinal extract, conventional pharmaceutical adjuvants are added in volatile oil clathrate compound, prepare plastic
Wafer, granule, tablet or mixture.
The preparation (nine) of 9 Chinese medicine composition of the present invention of embodiment
Each raw material medicines in portions by weight matches：10 parts of trigone, 10 parts of curcuma zedoary, 18 parts of the tuber of pinellia processed, 18 parts of Rhizoma Arisaematis (processed), Radix Astragali 20
Part, 20 parts of Rhizoma Atractylodis Macrocephalae.Preparation process is as follows：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 taste medicinal material weight 8 are added
The distilled water of times amount collected volatile oil using steam distillation refluxing extraction 5 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the distilled water of the 3 taste medicinal material total weight, 8 times of amounts, stir evenly, and decoct 2 times,
Each amount of water is 8 times of medicinal material total weight, 1 hour every time, merges decoction liquor, concentrates, dry, weighs, obtains medicinal extract；
B) volatile oil clathrate compound is prepared：Take the inclusion essential oil material hydroxy propyl-Beta-ring for being equivalent to volatilization 8 times of amounts of weight of oil
Appropriate distilled water is added in dextrin and beta-cyclodextrin, and saturated aqueous solution is configured at 50 DEG C, is stirred continuously down and is slowly added to volatilize
The absolute ethyl alcohol dilution of oil stirs 2 hours, is positioned over 4 DEG C of refrigerators 18 hours, takes out and filters, heavy with 50 DEG C of petroleum ethers
It forms sediment, it is dry, it weighs, obtains volatile oil clathrate compound；
C) in above-mentioned steps a) and b) obtained by medicinal extract, conventional pharmaceutical adjuvants are added in volatile oil clathrate compound, prepare plastic
Wafer, granule, tablet or mixture.
The influence that embodiment 10 expresses human gastric cancer MKN-45 transplanted tumor in nude mice size and PCNA and EGFR
One, experimental method
1. medicine preparation：
Chinese medicine of the present invention：Preparation process is as follows：A) traditional Chinese medicine extraction：Trigone 100g, curcuma zedoary 100g and Rhizoma Atractylodis Macrocephalae 200g are taken, suitably
It crushes in postposition extraction vessel, the distilled water of 3 taste medicinal material weight, 8 times of amounts is added, it is small using steam distillation refluxing extraction 5
When, collect volatile oil；The Radix Astragali 200g of pulverization process, Rhizoma Arisaematis (processed) 180g, tuber of pinellia 180g processed are added in extraction vessel, then added
The distilled water for entering the 3 taste medicinal material total weight, 8 times of amounts, stirs evenly, and decocts 2 times, each amount of water is the 8 of medicinal material total weight
Times, 1 hour every time, merge decoction liquor, concentrates, it is dry, it weighs, obtains medicinal extract；B) volatile oil clathrate compound is prepared：It takes to be equivalent to and wave
The inclusion essential oil material beta-cyclodextrin of 8 times of amounts of hair oil weight, is added appropriate distilled water, is configured to be saturated at 50 DEG C water-soluble
Liquid, is stirred continuously down the absolute ethyl alcohol dilution for being slowly added to volatile oil, stirs 2 hours, is positioned over 4 DEG C of refrigerators 18 hours, takes
Go out to filter, be precipitated with 50 DEG C of petroleum ethers, it is dry, it weighs, obtains volatile oil clathrate compound；C) gained in above-mentioned steps a) and b)
Medicinal extract, beta-cyclodextrin is added in volatile oil clathrate compound, mixing is dried, crushed into fine powder, and appropriate amount of ethanol is added to pelletize, dry, system
At 1000g.
Compare Chinese medicine one：Preparation process is as follows：A) scorpio 100g, centipede 100g, Rhizoma Arisaematis (processed) 180g, tuber of pinellia 180g processed are taken
It is added in extraction vessel, adds the distilled water of the 4 taste medicinal material total weight, 8 times of amounts, stir evenly, decoct 2 times, each amount of water
It is 8 times of medicinal material total weight, 1 hour every time, merges decoction liquor, concentrate, it is dry, it weighs, obtains medicinal extract；B) in above-mentioned steps
A) beta-cyclodextrin is added in the medicinal extract obtained by, mixing is dried, crushed into fine powder, and appropriate amount of ethanol is added to pelletize, dry, is made
1000g。
Compare Chinese medicine two：Preparation process is as follows：A) traditional Chinese medicine extraction：Rhizoma Atractylodis Macrocephalae 200g is taken, it is appropriate to crush in postposition extraction vessel,
The distilled water of 8 times of amounts of medicinal material weight are added, using steam distillation refluxing extraction 5 hours, collect volatile oil；By pulverization process
Scorpio 100g, centipede 100g, Radix Astragali 200g, Rhizoma Arisaematis (processed) 180g, tuber of pinellia 180g processed be added extraction vessel in, add this 5
The distilled water of 8 times of amounts of taste medicinal material total weight, stirs evenly, and decocts 2 times, each amount of water is 8 times of medicinal material total weight, every time
1 hour, merge decoction liquor, concentrates, it is dry, it weighs, obtains medicinal extract；B) volatile oil clathrate compound is prepared：It takes and is equivalent to volatile oil weight
The inclusion essential oil material beta-cyclodextrin of 8 times of amounts of amount, is added appropriate distilled water, saturated aqueous solution is configured at 50 DEG C, constantly
It is slowly added to the absolute ethyl alcohol dilution of volatile oil under stirring, stirs 2 hours, be positioned over 4 DEG C of refrigerators 18 hours, take out and filter,
It is precipitated with 50 DEG C of petroleum ethers, it is dry, it weighs, obtains volatile oil clathrate compound；C) in above-mentioned steps a) and b) obtained by medicinal extract,
Beta-cyclodextrin is added in volatile oil clathrate compound, mixing is dried, crushed into fine powder, and appropriate amount of ethanol is added to pelletize, dry, is made
1000g。
Compare Chinese medicine three：Preparation process is as follows：A) traditional Chinese medicine extraction：Take Radix Astragali 150g, Radix Codonopsis 150g, honeysuckle 150g, Pu
Public English 30g, radix bupleuri 30g, radix scutellariae 30g, Radix Paeoniae Alba 120g, acorus graminens soland 30g, trigone 90g, rhizoma arisaematis 90g, tuber of pinellia 90g, long-noded pit viper
Tongue grass 300g, Radix Notoginseng 30g, subprostrate sophora 90g, curcuma zedoary 90g are added in extraction vessel, add the 15 taste medicinal material total weight, 8 times of amounts
Distilled water, stir evenly, decoct 2 times, each amount of water is 8 times of medicinal material total weight, 1 hour every time, merges decoction liquor,
Concentration, it is dry, it weighs, obtains medicinal extract；B) beta-cyclodextrin is added in the medicinal extract obtained by above-mentioned steps a), mixing dry, pulverize
At fine powder, appropriate amount of ethanol is added to pelletize, it is dry, 1000g is made.
Positive control drug：5 FU 5 fluorouracil (fluorouracil, 5-FU) 10ml, 0.25g.
2. preparing inoculating cell suspension：Sterile working, when in vitro culture MKN-45 cell growths are intensive, 0.05% tryptose
Enzyme and 0.02% ethylenediamine tetra-acetic acid (ethylenediaminetet-raacetic acid, EDTA) digest 1~2min, pour out
The washing of 1640 liquid of serum-free is added in digestive juice, and centrifugation is made into inoculating cell with 1640 liquid of serum-free, and a concentration of 2 × 106/L.With
Phase contrast microscope observation is in uniform single cell suspension.
3. inoculating：Sterile working sterilizes skin, takes l ml syringes, pump out 0.4ml cell suspensions, be seeded in
Nude mice right fore root is subcutaneous.Nude mouse subcutaneous transplantation passes on, and takes the nude mouse of 14d or so after passing on, sterile working, from armpit
It is subcutaneous to strip tumor tissues, fibrous capsule is rejected, is cut, well-grown is chosen, in pale red, flesh of fish shape tumor tissue, is cut into 1mm
× 1mm × 1mm fritters are placed in spare in physiological saline.Nude mice claims after quality with 75mg/kg ketamine intraperitoneal injection of anesthesia, edge
Upper abdomen median line cuts about 1cm, and the tumor tissue got ready is implanted into.It is postoperative to observe tumour growth situation day by day, with mouse abdomen
There is accessible mass as tumor formation mark in portion, observes the variations such as mouse general activity and nutritional status.
4. experimental animal is grouped and medication：BABL/c nude mices after inoculation are randomly divided into 6 groups, respectively treatment group, in
One control group of medicine, two control group of Chinese medicine, three control group of Chinese medicine, chemotherapy group and lotus knurl control group, every group each 15, specific pathogen free
Sub-cage rearing is in laminar-flow rack under the conditions of bacterium (specificpathogen free, SPF).Administration is as follows：Treatment group：In inoculation
Next day starts every nude mice and gavages Chinese medicinal granule pure water lysate of the present invention afterwards, gives crude drug 9.2g/kg weights, 1 time/d, even
Continuous gavage 6 weeks.One control group of Chinese medicine：After inoculation next day start every nude mice gavage control one particle pure water lysate of Chinese medicine,
Give crude drug 9.2g/kg weights, 1 time/d, continuous gavage 6 weeks.Two control group of Chinese medicine：Next day starts every nude mice after inoculation
Control two particle pure water lysate of Chinese medicine is gavaged, gives crude drug 9.2g/kg weights, 1 time/d, continuous gavage 6 weeks.Chinese medicine three is right
According to group：After inoculation next day start every nude mice gavage control three particle pure water lysate of Chinese medicine, give crude drug 9.2g/kg constitutions
Amount, 1 time/d, continuous gavage 6 weeks.Chemotherapy group：5-FU, at 7.5mg/ml, is injected intraperitoneally, 1 with normal saline dilution by 60mg/kg
Times/week, continuous use 3 weeks.Lotus knurl control group：Next day starts every nude mice and gavages physiological saline after inoculation, 1 time/d, continuously
Gavage 6 weeks.
5. growth of transplanted human, mouse activity and nutrition condition observation：Mouse activity, feed and nutrition feelings are observed after inoculation
Condition.6 weeks post-tensioning necks put to death nude mice, subcutaneously completely strip tumor tissues from armpit, reject fibrous capsule, in being weighed on electronic balance
Tumor quality.Formula tumour inhibiting rate (%)=(average tumor quality after average tumor quality-medication group treatment after control group treatment)/
Average tumor quality × 100 calculate tumour inhibiting rate after control group treatment.The tumor tissue for completing to observe and weigh quality uses neutral formal
Woods is fixed, paraffin embedding, 5 μm of serial section.
6. transplanting the measurement of tumor tissue PCNA, EGFR：SP methods, Hematoxylin-eosin dyeing, paraffin section de-waxing to water are micro-
Wave repairs antigen.1 drop of primary antibody (mouse anti-human PCNA, EGFR), 4 DEG C of refrigerator overnights is added dropwise in every slice, and PBS is added dropwise after rinsing 3 times
Biotinylation secondary antibody, on every slice plus 1 drop streptavidin-Peroxidase Solution, 3,3'- diamino and connection
Aniline substrate colour developing 10min, dimethylbenzene is transparent, neutral gum closing.PCNA stained positive substances are in brown color, graininess, position
In in stomach cancer cell core, under × 200 oil mirrors, the cell of random counter 200 or more is as analysis radix, count tag PCNA
Index, i.e., labeled cell percentage shared in a cell mass.EGFR positive stainings are located on endochylema and after birth mostly, a
It Wei Yu not be brown yellow granule, score respectively by dye level and positive cell proportion on karyon.(1) dye level：0
Point, non-coloring；1 point, shallow coloring；It is 2 points, deep to colour.(2) positive cell ratio：0 point, non-coloring；1 point, coloring≤1/3；2 points,
1/3<Coloring≤2/3；3 points, coloring>2/3.Two results addeds>3 points are the positive.
Two, experimental result
1. the influence pair the growth of nude mice MKN-45 sdenocarcinomas of stomach
Lotus knurl control group 13d or so after MKN-45 people's sdenocarcinoma of stomach is inoculated in nude mice by subcutaneous, abdomen starts to touch nodular type
Lump gradually increases later；Remaining each group is later than control group and touches lump.Nude mice is put to death after 6 weeks, weighs tumor quality, is calculated
Tumour inhibiting rate, each group transplantable tumor tumor quality and tumour inhibiting rate statistical result are shown in Table 1.The result shows that each administration group treatment posterior tuberosity quality with
Lotus knurl control group compares, the statistically significant (P of difference<0.05)；Treatment group's tumor quality and one control group of Chinese medicine, Chinese medicine two are right
It is respectively compared according to group, three control group of Chinese medicine, the statistically significant (P of difference<0.05), compared with chemotherapy group, difference is without statistics
Learn meaning (P>0.05).
1 each group transplantable tumor tumor quality of table and tumour inhibiting rate
2. PCNA, EGFR are expressed in tumor tissue
PCNA positive expression rates statistical result is shown in Table 2 in each group tumor tissue.The result shows that the PCNA positive tables of each administration group
It is substantially less than lotus knurl control group (P up to rate<0.05), cell Proliferation liveness is decreased obviously after medicine taking prompting；Treatment group
PCNA positive expression rates are respectively compared with one control group of Chinese medicine, two control group of Chinese medicine, three control group of Chinese medicine, and difference has statistics
Meaning (P<0.05), compared with chemotherapy group, no significant difference (P>0.05).
PCNA positive expressions in 2 each group tumor tissue of table
| Group | n | Positive expression rate (%) |
| Lotus knurl control group | 15 | 67.94±18.66 |
| Treatment group | 14 | 10.74±2.59 |
| One control group of Chinese medicine | 15 | 32.76±8.80 |
| Two control group of Chinese medicine | 13 | 29.44±6.26 |
| Three control group of Chinese medicine | 15 | 51.30±10.47 |
| 5-FU | 15 | 10.78±2.45 |
EGFR positive rates statistical result is shown in Table 2 in each group tumor tissue.The result shows that the EGFR positive rates of each administration group are aobvious
It writes and is less than lotus knurl control group (P<0.05)；Treatment group's EGFR positive rates and one control group of Chinese medicine, two control group of Chinese medicine, Chinese medicine three are right
It is respectively compared according to group, the statistically significant (P of difference<0.05), compared with chemotherapy group, no significant difference (P>
0.05)。
EGFR positive expressions in 3 each group tumor tissue of table
| Group | n | It is positive | It is negative | Positive rate (%) |
| Lotus knurl control group | 15 | 13 | 2 | 86.67 |
| Treatment group | 14 | 4 | 10 | 28.57 |
| One control group of Chinese medicine | 15 | 8 | 7 | 53.33 |
| Two control group of Chinese medicine | 13 | 7 | 6 | 53.85 |
| Three control group of Chinese medicine | 15 | 10 | 5 | 66.67 |
| 5-FU | 15 | 4 | 11 | 26.67 |
The above result shows that Chinese medicine composition of the invention can significantly inhibit the life of nude mice MKN-45 sdenocarcinoma of stomach transplantable tumors
It is long, lower PCNA and EGFR expression in gastric cancer in nude mice tissue.
Embodiment 11 plants walker-256 rat stomach the inhibiting effect of tumor
One, experimental method
1. rat stomach plants tumor model foundation
Rat walker-256 ascites tumor models are initially set up, then extracting seroperitoneum with asepsis injector is inoculated in greatly
Armpit is subcutaneous on the right side of mouse, sees that right side armpit subcutaneously grows solid tumor after 7d.Stomach plants tumor：Above-mentioned subcutaneous solid tumor tissue is taken, by it
The tissue block of about 0.5mm × 0.5mm sizes is washed to the greatest extent and be cut into, abdomen is opened after Wistar rat anesthesias, tumor tissue block is inoculated in stomach
Wall placenta percreta, and instill OB biogums and be adhesively fixed.All rat record feeds daily, drinking-water situation, weigh per 3d, observe it
Spirit, the variation of activity situation, fur moisture.
2. experiment packet and intervention
40 rats, 36 are inoculated with tumor mass in stomach, remaining 4 rat not being inoculated with is Normal group, plants tumor mass
Wistar rats be randomly divided into three groups, every group 12：Treatment group：Give Chinese medicinal granule of the present invention (the same embodiment of preparation method
10) pure water lysate (being equivalent to crude drug 55g/kg weights), after modeling next day be administered immediately, daily gavage, successive administration
21d.Chinese medicine control group：It gives control Chinese medicine two particle (the preparation method is the same as that of Example 1 0) pure water lysate and (is equivalent to crude drug
55g/kg weights), after modeling next day be administered immediately, daily gavage, successive administration 21d.Model control group：Give physiology salt
Water about 3mL/ only, after modeling next day be administered immediately, daily gavage, successive administration 21d.Normal group only normal diet daily
Raising.It is raised under the conditions of all same SPF of rat.
3. tissue specimen samples
21d post-tensioning necks put to death all rats, open abdomen, observe intraperitoneal naked eyes visible change, ascites and Organ relative weight situation,
Stripping takes the fresh tumor tissue of each group stomach (it is a piece of not plant tumor rat gastric tissue).The tissue block formalin of taking-up, which is fixed, prepares system
Standby paraffin section (5 μm thick), immunohistochemistry detection.
4. Immunohistochemical Method detects
Stomach is planted CD34, α-Sma, FAP α protein expressions in tumor and gastric tissue sample and is detected using Immunohistochemical Method.
The fibroblast of CD34 (+) is distributed mainly on mucous layer and placenta percreta in alimentary canal, and CD34 is expressed in fibroblast film
On；FAP alpha selectives are expressed in malignant tumour matrix in tumour associated fibroblast cell, and main expression is in film and interstitial；α-
Sma is for identifying the most important marker of tumour associated fibroblast cell, and brown color is presented extremely on cell membrane in main expression
Brownish black is judged to the positive.5 visuals field of every slice, thin piece random acquisition, microscope is taken pictures, using 5 of Image J2x software collections
The positive area in integral optical density value/5 visual field at visual field positive staining position, the average optical density value for calculating 5 visuals field are
For its expression quantity.
Two, experimental result
Rat stomach plants CD34, α-Sma in tumor and gastric tissue, the expression statistical result of FAP α is shown in Table 4.The result shows that
Remaining each group rat CD34 expressions are significantly lower than rats in normal control group (P<0.05), the expression of FAP α and α-SMA are then bright
It is aobvious to be higher than Normal group (P ＜ 0.05)；The CD34 expressions for the treatment of group and Chinese medicine control rats are obviously higher than model
Control rats (P<0.05), the expression of FAP α and α-SMA are then significantly lower than model control group (P ＜ 0.05)；Treatment group rat
CD34 expressions be apparently higher than Chinese medicine control rats (P<0.05), the expression of FAP α and α-SMA are then significantly lower than Chinese medicine
Control group (P ＜ 0.05).
4 each group rat stomach of table plants the expression of CD34, α-Sma, FAP α in tumor and gastric tissue
| Group | Number of samples | CD34 | α-Sma | FAPα |
| Normal group | 4 | 809.52±11.68 | 647.85±20.46 | 620.73±10.29 |
| Model control group | 12 | 598.22±17.36 | 834.49±28.62 | 883.01±18.54 |
| Chinese medicine control group | 12 | 680.15±22.47 | 753.16±19.53 | 771.19±20.66 |
| Treatment group | 12 | 741.40±27.39 | 691.40±11.75 | 704.85±23.87 |
The above result shows that Chinese medicine composition of the invention can change tumor microenvironment, therefore tumour growth can be inhibited.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, under the premise of not departing from the method for the present invention, can also make several improvement and supplement, these are improved and supplement also should be regarded as
Protection scope of the present invention.
Claims (9)
1. a kind of antitumor Chinese medicine composition, which is characterized in that it is made of the bulk pharmaceutical chemicals of following parts by weight：Trigone 9-15
Part, 9-15 parts of curcuma zedoary, tuber of pinellia 15-20 parts processed, 15-20 parts of Rhizoma Arisaematis (processed), 15-30 parts of Radix Astragali, 15-30 parts of Rhizoma Atractylodis Macrocephalae.
2. Chinese medicine composition according to claim 1, which is characterized in that it is made of the bulk pharmaceutical chemicals of following parts by weight：Three
10-12 parts of rib, 10-12 parts of curcuma zedoary, tuber of pinellia 16-18 parts processed, 16-18 parts of Rhizoma Arisaematis (processed), 20-25 parts of Radix Astragali, 20-25 parts of Rhizoma Atractylodis Macrocephalae.
3. Chinese medicine composition according to claim 1, which is characterized in that it is made of the bulk pharmaceutical chemicals of following parts by weight：Three
10 parts of rib, 10 parts of curcuma zedoary, 18 parts of the tuber of pinellia processed, 18 parts of Rhizoma Arisaematis (processed), 20 parts of Radix Astragali, 20 parts of Rhizoma Atractylodis Macrocephalae.
4. according to any Chinese medicine compositions of claim 1-3, which is characterized in that the dosage form of the Chinese medicine composition is
Mixture, granule, capsule or tablet.
5. according to any Chinese medicine compositions of claim 1-3, which is characterized in that the Chinese medicine composition preparation method
Include the following steps：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 6-10 times of taste medicinal material weight are added
The distilled water of amount collected volatile oil using steam distillation refluxing extraction 4-8 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the 6-10 times of distilled water measured of 3 taste medicinal material total weight, stir evenly, and decoct 2-
3 times, each amount of water is 6-10 times of medicinal material total weight, 0.5-1.5 hours each, merges decoction liquor, is concentrated, dry, is claimed
Weight, obtains medicinal extract；
B) volatile oil clathrate compound is prepared：It takes and is equivalent to the inclusion essential oil material that 6-10 times of weight of oil of volatilization is measured, appropriate steam is added
Distilled water is configured to saturated aqueous solution at 40-60 DEG C, is stirred continuously down the absolute ethyl alcohol dilution for being slowly added to volatile oil, stirs
It mixes 1-3 hours, is positioned over 2-8 DEG C of refrigerator 12-24 hours, take out and filter, precipitated with 30-60 DEG C of petroleum ether, it is dry, claim
Weight, obtains volatile oil clathrate compound.
6. any Chinese medicine composition application in preparations of anti-tumor drugs of claim 1-3.
7. the application stated according to claim 6, which is characterized in that the tumour is gastric cancer, liver cancer, intestinal cancer, cancer of pancreas, gall-bladder
Cancer or the cancer of the esophagus.
8. the preparation method of any Chinese medicine compositions of claim 1-3, which is characterized in that the preparation method includes
Following steps：
A) traditional Chinese medicine extraction：Trigone, curcuma zedoary and Rhizoma Atractylodis Macrocephalae are taken, it is appropriate to crush in postposition extraction vessel, 3 6-10 times of taste medicinal material weight are added
The distilled water of amount collected volatile oil using steam distillation refluxing extraction 4-8 hours；By the Radix Astragali of pulverization process, day processed south
Star, the tuber of pinellia processed are added in extraction vessel, add the 6-10 times of distilled water measured of 3 taste medicinal material total weight, stir evenly, and decoct 2-
3 times, each amount of water is 6-10 times of medicinal material total weight, 0.5-1.5 hours each, merges decoction liquor, is concentrated, dry, is claimed
Weight, obtains medicinal extract；
B) volatile oil clathrate compound is prepared：It takes and is equivalent to the inclusion essential oil material that 6-10 times of weight of oil of volatilization is measured, appropriate steam is added
Distilled water is configured to saturated aqueous solution at 40-60 DEG C, is stirred continuously down the absolute ethyl alcohol dilution for being slowly added to volatile oil, stirs
It mixes 1-3 hours, is positioned over 2-8 DEG C of refrigerator 12-24 hours, take out and filter, precipitated with 30-60 DEG C of petroleum ether, it is dry, claim
Weight, obtains volatile oil clathrate compound.
9. the preparation method stated according to claim 8, which is characterized in that the inclusion essential oil material is selected from hydroxy propyl-Beta-
Cyclodextrin and/or beta-cyclodextrin.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710091604.5A CN108452240B (en) | 2017-02-20 | 2017-02-20 | Anti-tumor traditional Chinese medicine composition and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN115364186A (en) * | 2022-07-22 | 2022-11-22 | 中国中医科学院广安门医院 | Traditional Chinese medicine composition for esophageal cancer postoperation and application thereof |
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| CN103948859A (en) * | 2014-04-24 | 2014-07-30 | 上海中医药大学附属岳阳中西医结合医院 | Anti-tumor traditional Chinese medicine compound preparation as well as preparation method and application thereof |
| CN104147540A (en) * | 2014-08-28 | 2014-11-19 | 山东省千佛山医院 | Traditional Chinese medicine composition for treating malignant tumor |
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| CN103948859A (en) * | 2014-04-24 | 2014-07-30 | 上海中医药大学附属岳阳中西医结合医院 | Anti-tumor traditional Chinese medicine compound preparation as well as preparation method and application thereof |
| CN104147540A (en) * | 2014-08-28 | 2014-11-19 | 山东省千佛山医院 | Traditional Chinese medicine composition for treating malignant tumor |
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| CN115364186A (en) * | 2022-07-22 | 2022-11-22 | 中国中医科学院广安门医院 | Traditional Chinese medicine composition for esophageal cancer postoperation and application thereof |
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