CN108441441A - A kind of preparation method and application of the Leersia Sw endogenetic bacteria with reduction of hexavalent chromium - Google Patents
A kind of preparation method and application of the Leersia Sw endogenetic bacteria with reduction of hexavalent chromium Download PDFInfo
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Abstract
The invention discloses a kind of preparation method and applications of the Leersia Sw endogenetic bacteria with reduction of hexavalent chromium, the strain classification is named as bacillus (Bacillus sp.) Y04, China Committee for Culture Collection of Microorganisms's common micro-organisms center, address were preserved on 06 22nd, 2017：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, postcode：100101, deposit number is CGMCC NO.14266.The present invention is the Leersia Sw endogenetic bacteria separated from the leaf tissue of chromium hyperaccumulative plant Leersia Sw, is identified as bacillus, is named as Y04.Hexavalent chrome reduction can be trivalent chromium by the bacterial strain, and specify the condition of reduction of hexavalent chromium, and strain resource and theoretical foundation are provided for hexavalent chromium polluted microorganism remediation.
Description
Technical field
The present invention relates to a kind of bacterial strain can be used for environmental pollution improvement, it is related specifically to a kind of with reduction of hexavalent chromium
The preparation method and application of Leersia Sw endogenetic bacteria.
Background technology
With the development of state-of-the-art technology, heavy metal chromium is widely used in pharmacy, plating etc. are multi-field.But
At the same time, the pollution problem of the chromium and compound in environment is also increasingly severe.Long Term Contact chromium and chromium compound are easy
Gastrointestinal system, immune system, liver and kidney are had an impact and even induce respiratory system cancer.It is main in various chromium forms
Will in the form of chromate and dichromate ion existing for Cr (VI) be considered as that toxicity is strongest.In the world to the water body of pollution of chromium,
Soil, which is all advocated, uses biological restoration, i.e., by the removal huge sum of money the effects that flocculation of microorganism or plant, uptake and accumulation, enrichment
Belong to ion.The bacillus albus category (Leucobacter sp.) that is detached from the environment of chromium heavy metal pollution, pseudomonas
(pseudomonad), streptomyces (Streptomyces griseus.), bacillus (Bacillus sp.) and heat of dwelling
Pseudomonas (Thermusscotoductus) all has preferable Cr (VI) removal ability.
Super enriching plant have biomass it is big, be 10~500 times of conventional plant to heavy metal adsorption amount, can be heavy metal-polluted
The advantages such as well-grown in soil are contaminated, the application in environmental pollution reparation is increasingly extensive.At the same time, raw in super enriching plant
The research of bacterium also begins to be paid attention to by researcher, as As super enriching plants ciliate desert-grass, Zn super enriching plants Sedum alfredii Hance, Cd are super
Accumulate plant black nightshade, Mn hyperaccumulative plant Phytolacca acinosas etc. studies have reported that.Leersia Sw (Leersia hexandra Swartz) is
The chromium hyperaccumulative plant that Zhang Xuehong etc. has found in Guilin and the tired plant of the chromium ultraproduct that the first is found within Chinese territory
Object, research shows that it has stronger accumulation ability to Cr (III) and Cr (VI).But currently with Leersia Sw endogenetic bacteria pair
There is not been reported for the research that heavy metal Cr (VI) is restored.The present invention is hexavalent chromium polluted micro- life using Leersia Sw as raw material
Object reparation provides new microbial resources.
Invention content
The object of the present invention is to provide a kind of preparation method of Leersia Sw endogenetic bacteria with reduction of hexavalent chromium and
Using the bacterial strain has the function for trivalent chromium by hexavalent chrome reduction.
The technical solution adopted in the present invention is a kind of preparation method of the Leersia Sw endogenetic bacteria with reduction of hexavalent chromium,
The specific steps are：
(1) Leersia Sw plant is acquired, the appropriate disease-free Leersia Sw blade of health is chosen, distilled water flushing is clean；
(2) blade of Leersia Sw obtained by step (1) aseptically used to 70% alcohol immersion 20s, 2.5% time
Sodium chlorate solution impregnates 1min, then uses aseptic water washing 6 times；
(3) suitable leaf texture obtained by picking step (2), grinding, is inoculated in beef extract-peptone fluid nutrient medium,
37 DEG C, shaken cultivation 2d under the conditions of 120r/min；
(4) the beef extract-peptone solid containing Cr (VI) a concentration of 200mg/L is coated on after diluting step (3) gains
Culture medium, 37 DEG C of culture 2d, the preferable bacterium colony of picking growing way obtain pure bacterial strain through crossing repeatedly；
The Leersia Sw endogenetic bacteria, Classification And Nomenclature：Bacillus (Bacillus sp.) Y04, the bacterial strain in
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 06 22nd, 2017, deposit number is
CGMCC NO.14266, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
The preparation method of the Leersia Sw endogenetic bacteria with reduction of hexavalent chromium, which is characterized in that in step (1)
Used plant is the leaf portion of the chromium hyperaccumulative plant Leersia Sw in Environmental Science and Engineering institute of Guilin University of Technology laboratory
Tissue；
The preparation method of the Leersia Sw endogenetic bacteria with reduction of hexavalent chromium, which is characterized in that in step (3)
Fluid nutrient medium group become：Beef extract 3g/L, peptone 10g/L, NaCl 5g/L, remaining is water, pH 7.2；In step (4)
Solid medium group become：Beef extract 3g/L, peptone 10g/L, NaCl 5g/L, 15~20g/L of agar, remaining is water,
pH 7.2。
The application of the Leersia Sw endogenetic bacteria with reduction of hexavalent chromium, it is characterised in that Leersia Sw endogenetic bacteria exists
Application in reduction of hexavalent chromium.
The application of the Leersia Sw endogenetic bacteria with reduction of hexavalent chromium, which is characterized in that raw in the Leersia Sw
Initial pHs of the bacterium Y04 in reduction of hexavalent chromium is 4.0~6.0.
The application of the Leersia Sw endogenetic bacteria with reduction of hexavalent chromium, which is characterized in that raw in the Leersia Sw
The temperature of bacterium Y04 reduction of hexavalent chromium is 35~37 DEG C.
Description of the drawings
The growing state comparison diagram of Y04 bacterial strains under Fig. 1 differences Cr (VI) concentration
Influence diagrams of the initial pH of Fig. 2 to Y04 bacterial strain reduction of hexavalent chromium
Influence diagram of Fig. 3 temperature to Y04 bacterial strain reduction of hexavalent chromium
Influence diagram of Fig. 4 Cr (VI) initial concentrations to Y04 bacterial strain reduction of hexavalent chromium
Influence diagram of Fig. 5 reaction time to Y04 bacterial strain reduction of hexavalent chromium
Specific implementation mode：
In order to keep the present invention easier to understand, with reference to specific embodiment, the present invention is further explained.It should be understood that this
A little embodiments are merely to illustrate the invention rather than limit the scope of the invention.Unmentioned specific experiment in the following example
Method is usually carried out according to routine experiment method.
1 Leersia Sw chromium of embodiment restores the separation screening of endogenetic bacteria
Acquisition Guilin University of Technology's Environmental Science and Engineering institute phytoremediation laboratory Leersia Sw plant first takes appropriate
The Leersia Sw blade of health after being rinsed with water totally, impregnates 40s, then with 2.5% chlorine under aseptic condition with 70% alcohol
Sour sodium impregnates 2min, finally uses aseptic water washing 6 times, and removal is attached to the disinfectant of material surface.With last under aseptic condition
The sterile water of a time flushing is coated on beef extract-peptone solid plate culture medium, is grown without microorganism after culture, shows surface
Disinfection is thorough.The leaf tissue through surface sterilization in right amount is taken under aseptic condition, and the sodium chloride solution of 1mL 0.9% is added fully to grind
Mill, takes 1mL lapping liquids to be inoculated in 100mL beef extract-peptone fluid nutrient mediums (500mL triangular flasks), 37 DEG C, 120r
min-1Under the conditions of shaken cultivation 2d, by culture solution press 10-2, 10-3, 10-4, 10-5, 10-6It is diluted, takes 20 μ L to use respectively flat
It is respectively 37 DEG C of constant temperature trainings on the beef extract-peptone plating medium of 200mg/L that plate coater, which is coated on containing Cr (VI) concentration,
After supporting 24~48h, according to bacterium colony growing state, the bacterium colony that growing way is preferable, resistance to chromium performance is strong is selected, accesses in tablet containing chromium, adopts
With method of scoring carry out it is multiple isolate and purify, obtain Cr (VI) resistance endophyte pure culture.
The identification of anti-Cr (VI) bacterial reduction：After the bacterial strain activation that test tube slant is preserved, one ring of picking, which is inoculated in, to be contained
In the 50mL triangular flasks of 20mL fluid nutrient mediums, 37 DEG C, 120r/min shaken cultivations for 24 hours afterwards be used as seed liquor.Seed liquor is pressed
10% inoculum concentration is inoculated in the fluid nutrient medium (20mL/ of a concentration of 50mg/L of Cr (VI), 100 mg/L and 200mg/L respectively
50mL triangular flasks) in, it is placed in 37 DEG C, shaken cultivation in 120r/min incubators.Every sampling 1mL under aseptic condition for 24 hours,
8000r/min centrifuges 10min, and supernatant is taken to measure Cr (VI) and total Cr concentration.Not to be inoculated with but contain the blank culture of Cr (VI)
Base is as blank control.Y04 bacterial strains are as shown in table 1 to the reduction effect of Cr VI.
Removal rate of the Y04 bacterial strains to chromium under table 1 difference Cr (VI) concentration difference incubation time
Influence of embodiment 2 Cr (VI) concentration to Y04 strain growths
After the bacterial strain activation that test tube slant is preserved, one ring of picking is inoculated in 100 mL triangles of the fluid nutrient medium containing 40mL
Bottle in, 37 DEG C, 120r/min shaken cultivations for 24 hours afterwards be used as seed liquor.Seed liquor is inoculated in Cr respectively by 10% inoculum concentration
(VI) the beef extract-peptone fluid nutrient medium (100mL/250mL triangular flasks) of a concentration of 50mg/L, 100mg/L and 200mg/L
In, it is placed in 120 r/min shaken cultivations on 37 DEG C of constant temperature horizontal shaker.During culture, under separated in time aseptic condition
5mL is sampled, the optical density OD values under 600nm wavelength are measured.As a result as shown in Fig. 1.When Cr (VI) is a concentration of in culture medium
When 50mg/L and 100mg/L, in preceding 6h, the growth tendency of bacterial strain is similar with the blank for being not added with Cr (VI), but the speed of growth has
Slowed down, and is increased as the increase of Cr (VI) concentration slows down degree.After 6h, Fungal biodiversity declines, this may be due to bacterium
There are certain lag phases for response of the strain to Cr (VI) toxicity；Thereafter the adaptation due to bacterial strain to Cr (VI) or antidotal effect, bacterium
Strain continues slowly growth.As Cr (VI) a concentration of 200mg/L in culture medium, the growth of bacterial strain is greatly suppressed, until
After for 24 hours, bacterial strain just starts to grow.
Influences of the 3 initial pH of embodiment to Y04 bacterial strains reduction Cr (VI)
The bacterial strain Y04 that test tube slant preserves is activated through beef extract-peptone solid medium tablets, in 37 DEG C of constant temperature incubations
After being cultivated for 24 hours in case, 2 ring of picking is inoculated into the 250mL triangular flasks of the fluid nutrient medium of beef extract-peptone containing 100mL, 37 DEG C,
120r/min shaken cultivations are used as seed liquor afterwards for 24 hours.
Seed liquor is inoculated in the beef extract-peptone Liquid Culture containing Cr (VI) a concentration of 100mg/L by 15% inoculum concentration
In base (liquid amount 100mL/250mL triangular flasks), after adjusting pH with the sodium hydroxide and hydrochloric acid solution of 2M, eight layers of gauze sealing,
It is placed in 120r/min shaken cultivations on 37 DEG C of constant temperature horizontal shaker.After cultivating 48h, appropriate culture solution is sampled under aseptic condition,
10000r/min centrifuges 10min, by precipitation and separation of the supernatant.Precipitation distilled water solution mixing, measures OD600, supernatant survey
Determine the concentration of the concentration and total Cr of Cr (VI).As a result as shown in Fig. 2.
Influence of 4 temperature of embodiment to Y04 bacterial strains reduction Cr (VI)
The bacterial strain Y04 that test tube slant preserves is activated through beef extract-peptone solid medium tablets, in 37 DEG C of constant temperature incubations
After being cultivated for 24 hours in case, 2 ring of picking is inoculated into the 250mL triangular flasks of the fluid nutrient medium of beef extract-peptone containing 100mL, 37 DEG C,
120r/min shaken cultivations are used as seed liquor afterwards for 24 hours.
Seed liquor is inoculated in by 15% inoculum concentration and (uses the hydroxide of 2M containing Cr (VI) a concentration of 100mg/L, pH for 5.0
Sodium and hydrochloric acid solution are adjusted) beef extract-peptone fluid nutrient medium (liquid amount 100mL/250 mL triangular flasks) in, eight layers of yarn
Cloth seals, and is placed in 120r/min shaken cultivations on the constant temperature horizontal shaker of different temperatures.After cultivating 48h, sampled under aseptic condition
Appropriate culture solution, 10000r/min centrifuge 10min, by precipitation and separation of the supernatant.Precipitation distilled water solution mixing, measures
OD600, the concentration of the concentration and total Cr of supernatant measurement Cr (VI).As a result as shown in Fig. 3.
Influence of 5 initial Cr (VI) concentration of embodiment to Y04 bacterial strains reduction Cr (VI)
The bacterial strain Y04 that test tube slant preserves is activated through beef extract-peptone solid medium tablets, in 37 DEG C of constant temperature incubations
After being cultivated for 24 hours in case, 2 ring of picking is inoculated into the 250mL triangular flasks of the fluid nutrient medium of beef extract-peptone containing 100mL, 37 DEG C,
120r/min shaken cultivations are used as seed liquor afterwards for 24 hours.
Seed liquor is inoculated in by 15% inoculum concentration and (uses the sodium hydroxide and salt of 2M containing different Cr (VI) concentration, pH for 5.0
Acid solution is adjusted) beef extract-peptone fluid nutrient medium (liquid amount 100mL/250mL triangular flasks) in, the sealing of eight layers of gauze,
It is placed in 120r/min shaken cultivations on 37 DEG C of constant temperature horizontal shaker.After cultivating 48h, appropriate culture solution is sampled under aseptic condition,
10000r/min centrifuges 10min, by precipitation and separation of the supernatant.Precipitation distilled water solution mixing, measures OD600, supernatant survey
Determine the concentration of the concentration and total Cr of Cr (VI).As a result as shown in Fig. 4.
Influence of 6 reaction time of embodiment to Y04 bacterial strains reduction Cr (VI)
The bacterial strain Y04 that test tube slant preserves is activated through beef extract-peptone solid medium tablets, in 37 DEG C of constant temperature incubations
After being cultivated for 24 hours in case, 2 ring of picking is inoculated into the 250mL triangular flasks of the fluid nutrient medium of beef extract-peptone containing 100mL, 37 DEG C,
120r/min shaken cultivations are used as seed liquor afterwards for 24 hours.
Seed liquor is inoculated in by 15% inoculum concentration and (uses the sodium hydroxide and hydrochloric acid of 2M containing different Cr (VI) concentration, pH for 5
Solution is adjusted) beef extract-peptone fluid nutrient medium (liquid amount 100mL/250mL triangular flasks) in, the sealing of eight layers of gauze is set
In 120r/min shaken cultivations on 37 DEG C of constant temperature horizontal shaker.Appropriate culture is sampled after cultivating different time, under aseptic condition
Liquid, 10000r/min centrifuge 10min, by precipitation and separation of the supernatant.Precipitation distilled water solution mixing, measures OD600, supernatant
Liquid measures the concentration of the concentration and total Cr of Cr (VI).As a result as shown in Fig. 5.
Claims (6)
1. a kind of preparation method of the Leersia Sw endogenetic bacteria with reduction of hexavalent chromium, it is characterised in that the specific steps are：
(1) Leersia Sw plant is acquired, the appropriate disease-free Leersia Sw blade of health is chosen, distilled water flushing is clean；
(2) blade of Leersia Sw obtained by step (1) is aseptically impregnated into 20s, 2.5% sodium hypochlorite with 70% alcohol
Solution impregnates 1min, then uses aseptic water washing 6 times；
(3) suitable leaf texture obtained by picking step (2), grinding, is inoculated in beef extract-peptone fluid nutrient medium, 37
DEG C, shaken cultivation 2d under the conditions of 120r/min；
(4) the beef extract-peptone solid culture containing Cr (VI) a concentration of 200mg/L is coated on after diluting step (3) gains
Base, 37 DEG C of culture 2d, the preferable bacterium colony of picking growing way obtain Leersia Sw endogenetic bacteria through crossing repeatedly；
The Leersia Sw endogenetic bacteria, Classification And Nomenclature：Bacillus (Bacillus sp.) Y04, the bacterial strain is in 2017
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC within 22 days 06 month
NO.14266, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
2. the preparation method of Leersia Sw endogenetic bacteria according to claim 1, which is characterized in that used in step (1)
Plant be Environmental Science and Engineering institute of Guilin University of Technology laboratory chromium hyperaccumulative plant Leersia Sw leaf portion tissue.
3. the preparation method of Leersia Sw endogenetic bacteria according to claim 1, which is characterized in that the liquid in step (3)
Culture medium group becomes：Beef extract 3g/L, peptone 10g/L, NaCl 5g/L, remaining is water, pH 7.2；Solid in step (4)
Culture medium group becomes：Beef extract 3g/L, peptone 10g/L, NaCl 5g/L, 15~20g/L of agar, remaining is water, pH 7.2.
4. having the application of the Leersia Sw endogenetic bacteria of reduction of hexavalent chromium, feature according to claim 1-3 any one of them
It is application of the Leersia Sw endogenetic bacteria in reduction of hexavalent chromium.
5. the application of the Leersia Sw endogenetic bacteria according to claim 4 with reduction of hexavalent chromium, which is characterized in that described
Initial pHs of the Leersia Sw endogenetic bacteria Y04 in reduction of hexavalent chromium is 4.0~6.0.
6. the application of the Leersia Sw endogenetic bacteria according to claim 4 with reduction of hexavalent chromium, which is characterized in that described
The temperature of Leersia Sw endogenetic bacteria Y04 reduction of hexavalent chromium is 35~37 DEG C.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110282759A (en) * | 2019-08-15 | 2019-09-27 | 桂林理工大学 | A method of utilizing chromium in Bacillus cercus and Leersia Sw reciprocation purifying water body |
| CN110669706A (en) * | 2019-11-26 | 2020-01-10 | 桂林理工大学 | Leersia hexandra endophytic bacterium capable of reducing hexavalent chromium as well as preparation method and application thereof |
| CN112159786A (en) * | 2020-11-04 | 2021-01-01 | 河北科技大学 | Cr (VI) reducing strain C6, and culture condition and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684453A (en) * | 2009-07-03 | 2010-03-31 | 中南大学 | Bacterial strain for repairing chromium slag contaminated site |
| CN102676423A (en) * | 2012-03-20 | 2012-09-19 | 河南省农业科学院 | Reductive chromium ion bacillus thuringiensis YB-03 and cultivation method and application of reductive chromium ion bacillus thuringiensis YB-03 |
| CN104711212A (en) * | 2015-03-11 | 2015-06-17 | 华中农业大学 | Bacillus thuringiensis L68 with chromium reduction and fixation effects and application |
| CN106676045A (en) * | 2017-02-16 | 2017-05-17 | 武汉科技大学 | Antibiotic-resistant hexavalent chromium reducing bacterium and application thereof |
-
2018
- 2018-03-12 CN CN201810198416.7A patent/CN108441441B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684453A (en) * | 2009-07-03 | 2010-03-31 | 中南大学 | Bacterial strain for repairing chromium slag contaminated site |
| CN102676423A (en) * | 2012-03-20 | 2012-09-19 | 河南省农业科学院 | Reductive chromium ion bacillus thuringiensis YB-03 and cultivation method and application of reductive chromium ion bacillus thuringiensis YB-03 |
| CN104711212A (en) * | 2015-03-11 | 2015-06-17 | 华中农业大学 | Bacillus thuringiensis L68 with chromium reduction and fixation effects and application |
| CN106676045A (en) * | 2017-02-16 | 2017-05-17 | 武汉科技大学 | Antibiotic-resistant hexavalent chromium reducing bacterium and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| NEHA UPADHYAY ET AL.,: "Tolerance and Reduction of Chromium(VI) by Bacillus sp. MNU16 Isolated from Contaminated Coal Mining Soil", 《FRONTIERS IN PLANT SCIENCE》 * |
| 焦仕林等: "蜡样芽孢杆菌还原六价铬效果分析", 《中国公共卫生》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110282759A (en) * | 2019-08-15 | 2019-09-27 | 桂林理工大学 | A method of utilizing chromium in Bacillus cercus and Leersia Sw reciprocation purifying water body |
| CN110282759B (en) * | 2019-08-15 | 2021-12-14 | 桂林理工大学 | Method for purifying chromium in water body by utilizing interaction of bacillus cereus and Leersia hexandra Swartz |
| CN110669706A (en) * | 2019-11-26 | 2020-01-10 | 桂林理工大学 | Leersia hexandra endophytic bacterium capable of reducing hexavalent chromium as well as preparation method and application thereof |
| CN110669706B (en) * | 2019-11-26 | 2022-12-27 | 桂林理工大学 | Leersia hexandra endophytic bacterium capable of reducing hexavalent chromium and preparation method and application thereof |
| CN112159786A (en) * | 2020-11-04 | 2021-01-01 | 河北科技大学 | Cr (VI) reducing strain C6, and culture condition and application thereof |
| CN112159786B (en) * | 2020-11-04 | 2022-03-01 | 河北科技大学 | Cr (VI) reducing strain C6, and culture condition and application thereof |
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