CN108434398B

CN108434398B - Traditional Chinese medicine composition for treating lung cancer and preparation method and application thereof

Info

Publication number: CN108434398B
Application number: CN201810444042.2A
Authority: CN
Inventors: 陈涛
Original assignee: China Three Gorges University CTGU
Current assignee: China Three Gorges University CTGU
Priority date: 2018-05-10
Filing date: 2018-05-10
Publication date: 2021-02-02
Anticipated expiration: 2038-05-10
Also published as: CN108434398A

Abstract

The invention discloses a traditional Chinese medicine composition for treating lung cancer, a preparation method and an application thereof, wherein the composition comprises the following components in parts by weight: 15-20 parts of rhizoma paridis, 15-20 parts of oldenlandia diffusa, 15-20 parts of raw oyster, 9-15 parts of rhizoma pinellinae praeparata, 9-15 parts of thunberg fritillary bulb, 9-15 parts of pericarpium trichosanthis, 9-15 parts of hairyvein agrimony, 15-30 parts of semen coicis, 9-15 parts of selfheal, 9-12 parts of curcuma zedoary, 9-12 parts of pericarpium citri reticulatae, 9-12 parts of platycodon grandiflorum, 15-20 parts of Indian iphigenia bulb, 15-20 parts of radix pseudostellariae, 9-15 parts of adenophora tetraphylla, 9-15 parts of radix glehniae. It takes strengthening body resistance to eliminate pathogenic factors and treats both principal and secondary aspect of disease as the therapeutic principle. Through clinical observation, the total effective rate reaches 100%, and the traditional Chinese medicine composition is safe, effective, low in cost, convenient to master and worthy of clinical popularization and application.

Description

Traditional Chinese medicine composition for treating lung cancer and preparation method and application thereof

Technical Field

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating lung cancer and a preparation method and application thereof.

Background

Lung cancer is a malignant disease with cough, hemoptysis, chest pain, fever, and shortness of breath as its main clinical manifestations. The incidence and the fatality rate of the cancer are at the first place of malignant tumors. Histological subtypes of lung cancer mainly include non-small cell lung cancer and small cell lung cancer, accounting for 85% and 15% of lung cancer, respectively. According to literature reports, the incidence of lung cancer is continuously increased in recent years due to pathogenic factors such as smoking, air pollution, indoor environmental pollution and the like. To date, western medicine treatment has been surgical, chemotherapy, targeted medicine, and other therapeutic approaches. However, the total cure rate of lung cancer patients is less than 20 percent in the existing treatment mode, the 5-year survival rate of patients in advanced stage is less than 5 percent, the treatment effect is not ideal, and the life quality of the patients is affected due to the toxic and side effects of the medicine and high cost.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition for treating lung cancer, a preparation method and application thereof.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a traditional Chinese medicine composition for treating lung cancer comprises the following components in parts by weight: 15-20 parts of rhizoma paridis, 15-20 parts of oldenlandia diffusa, 15-20 parts of raw oyster, 9-15 parts of rhizoma pinellinae praeparata, 9-15 parts of thunberg fritillary bulb, 9-15 parts of pericarpium trichosanthis, 9-15 parts of hairyvein agrimony, 15-30 parts of semen coicis, 9-15 parts of selfheal, 9-12 parts of curcuma zedoary, 9-12 parts of pericarpium citri reticulatae, 9-12 parts of platycodon grandiflorum, 15-20 parts of Indian iphigenia bulb, 15-20 parts of radix pseudostellariae, 9-15 parts of adenophora tetraphylla, 9-15 parts of radix glehniae.

In a further preferable scheme, the traditional Chinese medicine composition comprises 15 parts of rhizoma paridis, 15 parts of oldenlandia diffusa, 15 parts of raw oyster, 10 parts of rhizoma pinellinae praeparata, 15 parts of thunberg fritillary bulb, 15 parts of pericarpium trichosanthis, 15 parts of hairyvein agrimony, 30 parts of semen coicis, 15 parts of selfheal, 10 parts of curcuma zedoary, 10 parts of dried orange peel, 10 parts of platycodon grandiflorum, 15 parts of edible tulip, 15 parts of radix pseudostellariae, 15 parts of raw astragalus membranaceus, 15 parts of radix adenophor.

Furthermore, the traditional Chinese medicine composition is prepared into liquid medicine, granules, plaster or other pharmaceutically acceptable dosage forms after extracting effective substances from the raw material components.

The invention also relates to a process for preparing said composition comprising the steps of: mixing all the raw materials in proportion, adding water to submerge the medicinal materials, decocting with slow fire until the water is boiled, decocting for 20 minutes, filtering to obtain medicinal juice, repeating the decocting and filtering processes twice, and mixing the obtained medicinal juice to obtain the decoction of the traditional Chinese medicine composition for treating lung cancer.

The invention also relates to application of the traditional Chinese medicine composition in preparing a medicine for treating lung cancer.

Lung cancer belongs to the category of diseases such as 'lung accumulation', 'lump in the abdomen', 'cough', 'hemoptysis' and 'chest pain' in the traditional Chinese medicine. The disease is located in the lung, but it is not treated because of the deficiency of spleen and stomach, failure of stomach qi, and exhaustion of food qi; in addition, lung-qi and kidney-yin are involved in the generation of both mother and child, and the physiological and pathological relationships of both are closely related to spleen and kidney. The pathogenesis of the disease is complex, most doctors believe that the disease is caused by the combined action of various factors such as internal factors (deficiency of vital qi), external factors (six excesses, pathogenic toxin, seven emotions, internal injury, overeating), and the like, but the focus of each family is different, and the view is not unified. The treatment should be based on the therapeutic principle of strengthening healthy qi to eliminate pathogenic factors and treating both principal and secondary aspect of disease.

The rhizoma paridis used in the invention is the dried rhizome of paris polyphylla which belongs to the genus paris of the family liliaceae. Rhizoma paridis has effects of clearing heat and toxic substances, relieving swelling and pain, cooling liver and arresting convulsion.

The oldenlandia diffusa used in the invention is a whole plant of a plant of the genus erysiphe of the family rubiaceae. The oldenlandia has the efficacies of clearing away heat and toxic material, promoting diuresis and treating stranguria.

The raw oyster used in the invention is the shell of Ostrea gigas, Ostrea gigas or Ostrea rivularis of Ostreidae. The raw oyster has the functions of calming the liver and suppressing yang; heavy-weight tranquilizing; softening and resolving hard mass; astringing and inducing astringency.

The rhizoma pinelliae praeparata used in the invention is tuber of pinellia ternata of Araceae. Rhizoma Pinelliae Preparata has effects of eliminating dampness and phlegm.

The thunberg fritillary bulb used in the invention is the bulb of the thunberg fritillary bulb which belongs to the functional and functional family of lily. Zhejiang fritillaria has the effects of clearing heat, eliminating phlegm, dissipating stagnation and eliminating carbuncle.

The pericarpium trichosanthis used in the invention is the pericarp of the plants of the Cucurbitaceae, namely the trichosanthes kirilowii Maxim and the trichosanthes kirilowii Maxim. The pericarpium Trichosanthis has effects of clearing lung-heat, eliminating phlegm, promoting qi circulation, relieving chest stuffiness and resolving hard mass.

The agrimony used in the invention is the overground part of the Agrimonia pilosa L of the Rosaceae family. Herba et Gemma Agrimoniae has effects of astringing to stop bleeding, removing toxic substance, treating sore, killing parasite, astringing sweat, and relieving cough.

The coix seed used in the invention is a dry mature seed of the coix seed of the gramineous plant. The coix seed has the effects of inducing diuresis to alleviate edema, excreting dampness, strengthening spleen, removing arthralgia, clearing heat and expelling pus.

The selfheal used by the invention is dry fruit cluster of the selfheal of the labiatae family. The selfheal has the effects of clearing heat and purging fire, improving eyesight, and eliminating stagnation and swelling.

The zedoary is the rhizome of curcuma zedoaria of Zingiberaceae. Rhizoma Curcumae has effects of activating qi-flowing, resolving stagnation, removing blood stasis, and relieving pain.

The pericarpium Citri Tangerinae used in the invention is dry mature pericarp of Rutaceae plant tangerine and its cultivar. The pericarpium Citri Tangerinae has effects of regulating qi-flowing, invigorating spleen, eliminating dampness and eliminating phlegm.

The platycodon grandiflorum used in the invention is a dried root of platycodon grandiflorum of the family of platycodonaceae. Radix Platycodi has effects of dispersing lung qi, relieving sore throat, eliminating phlegm, and expelling pus.

The Pseudobulbus Cremastrae Seu pleiones used in the invention is dry pseudobulb of Rhododendron pulchrum, Pleione bulbocodioides or Pleione yunnanensis of Orchidaceae. The Pseudobulbus Cremastrae Seu pleiones has effects of clearing heat and detoxicating, eliminating phlegm and resolving masses.

The radix pseudostellariae used in the invention is a dried root tuber of a Caryophyllaceae plant, namely, pseudostellaria heterophylla. Radix Pseudostellariae has effects of invigorating qi and spleen, promoting fluid production and moistening lung.

The radix astragali used in the invention is the root of Astragalus membranaceus bge and Astragalus membranaceus bge of Astragalus of Leguminosae. Radix astragali has effects of invigorating qi, consolidating superficial resistance, promoting urination, expelling toxin, expelling pus, healing sore, and promoting granulation.

The Adenophora tetraphylla of the present invention is dry root of Adenophora tetraphylla of the family Campanulaceae. The radix adenophorae has the effects of nourishing yin, clearing away lung-heat, benefiting stomach, promoting fluid production, reducing phlegm and tonifying qi.

The radix glehniae used in the invention is the root of glehnia littoralis which is an umbelliferae plant. Radix Glehniae has effects of nourishing yin, clearing away lung-heat, benefiting stomach and promoting fluid production.

In the formula, raw oysters are selected to have strong hardness softening and resolving power; rhizoma Pinelliae Preparata emphasizes drying dampness and eliminating phlegm with small toxicity, and radix astragali emphasizes strengthening body resistance and expelling toxin, and is more suitable for treating lung cancer.

The traditional Chinese medicine composition has the advantages of accurate formula, exact curative effect, simplicity, convenience, low cost and the like. Radix Adenophorae, radix Glehniae, radix Pseudostellariae, pericarpium Citri Tangerinae, and radix astragali are used for strengthening body resistance, and the rest for purgation. Wherein rhizoma paridis and herba Hedyotidis Diffusae have effects of clearing away heat and toxic materials, Concha Ostreae, rhizoma Pinelliae Preparata, Bulbus Fritillariae Thunbergii, Prunellae Spica for softening and resolving hard mass, pericarpium Citri Tangerinae, radix Platycodi, pericarpium Trichosanthis, Coicis semen, radix Platycodi, Curcumae rhizoma for promoting qi circulation and eliminating phlegm, radix Pseudostellariae, radix astragali, radix Adenophorae, radix Glehniae, and herba et Gemma Agrimoniae for invigorating qi and spleen. The medicines are combined and have synergistic effect, and the effects of clearing heat and removing toxicity, softening hardness and dissipating stagnation, tonifying qi and spleen, nourishing yin and promoting the production of body fluid are achieved together. The technology has the advantages of scientific formula, obvious curative effect, dialectical administration, clear mechanism and the like. Clinical observation shows that the invention has the effect of delaying tumor progression and even reversing tumor progression for lung cancer patients. The traditional Chinese medicines are common traditional Chinese medicines, and the administration mode is simple, so the traditional Chinese medicine is suitable for popularization and application in primary hospitals, particularly in underdeveloped areas.

Drawings

FIG. 1: MTT method for detecting influence of traditional Chinese medicine composition on A549 cell proliferation (note: comparison between groups under same concentration)^#P＜0.05；^##P＜0.01)。

FIG. 2: MTT method for detecting influence of traditional Chinese medicine composition on H1299 cell proliferation (injection: comparison between groups under same concentration)^#P＜0.05；^##P＜0.01)。

FIG. 3: the influence of the traditional Chinese medicine composition on the migration capacity of the human lung cancer cell A549 (the traditional Chinese medicine composition is low: 0.5mg/ml, the traditional Chinese medicine composition is high: 1mg/ml, and a control group is DDP1 ug/ml).

FIG. 4: the influence of the traditional Chinese medicine composition on the migration capacity of human lung cancer cells H1299 (the traditional Chinese medicine composition is low in content of 0.5mg/ml, the traditional Chinese medicine composition is high in content of 1mg/ml, and a control group is DDP1 ug/ml).

FIG. 5: the morphological change of the human lung cancer cell A549 after the traditional Chinese medicine composition acts (4 times, 10 times, 20 times and 40 times of magnification under a light microscope).

FIG. 6: the morphological change of human lung cancer cell H1299 (4 times, 10 times, 20 times and 40 times of magnification under a light microscope) after the traditional Chinese medicine composition acts.

Detailed Description

The invention will be further elucidated with reference to the following examples. These examples are to be construed as merely illustrative and not limitative of the remainder of the disclosure. After reading the description of the invention, one skilled in the art can make various changes and modifications to the invention, and such equivalent changes and modifications also fall into the scope of the invention defined by the claims.

Example 1:

a traditional Chinese medicine composition for treating lung cancer comprises the following components in parts by weight: 15 parts of rhizoma paridis, 15 parts of oldenlandia diffusa, 15 parts of raw oyster, 10 parts of rhizoma pinellinae praeparata, 15 parts of thunberg fritillary bulb, 15 parts of snakegourd peel, 15 parts of hairyvein agrimony, 30 parts of coix seed, 15 parts of selfheal, 10 parts of curcuma zedoary, 10 parts of dried orange peel, 10 parts of platycodon grandiflorum, 15 parts of edible tulip, 15 parts of radix pseudostellariae, 20 parts of raw astragalus membranaceus, 15 parts of adenophora tetraphylla and 15 parts of.

Example 2: a traditional Chinese medicine composition for treating lung cancer comprises the following components in parts by weight: 20 parts of rhizoma paridis, 15 parts of oldenlandia diffusa, 15 parts of raw oyster, 10 parts of rhizoma pinellinae praeparata, 15 parts of thunberg fritillary bulb, 15 parts of snakegourd peel, 15 parts of hairyvein agrimony, 30 parts of coix seed, 15 parts of selfheal, 10 parts of curcuma zedoary, 10 parts of dried orange peel, 10 parts of platycodon grandiflorum, 15 parts of edible tulip, 15 parts of radix pseudostellariae, 20 parts of raw astragalus membranaceus, 15 parts of adenophora tetraphylla and 15 parts of.

Example 3: a traditional Chinese medicine composition for treating lung cancer comprises the following components in parts by weight: 15 parts of rhizoma paridis, 15 parts of oldenlandia diffusa, 15 parts of raw oyster, 10 parts of rhizoma pinellinae praeparata, 15 parts of thunberg fritillary bulb, 15 parts of snakegourd peel, 15 parts of hairyvein agrimony, 15 parts of coix seed, 15 parts of selfheal, 10 parts of curcuma zedoary, 10 parts of dried orange peel, 10 parts of platycodon grandiflorum, 15 parts of edible tulip, 15 parts of radix pseudostellariae, 20 parts of raw astragalus membranaceus, 15 parts of adenophora tetraphylla and 15 parts of.

Example 4: a traditional Chinese medicine composition for treating lung cancer comprises the following components in parts by weight: 20 parts of rhizoma paridis, 15 parts of oldenlandia diffusa, 15 parts of raw oyster, 12 parts of rhizoma pinellinae praeparata, 15 parts of thunberg fritillary bulb, 15 parts of snakegourd peel, 15 parts of hairyvein agrimony, 30 parts of coix seed, 15 parts of selfheal, 10 parts of curcuma zedoary, 10 parts of dried orange peel, 10 parts of platycodon grandiflorum, 15 parts of edible tulip, 15 parts of radix pseudostellariae, 15 parts of raw astragalus membranaceus, 15 parts of adenophora tetraphylla and 15 parts of.

Example 5: a traditional Chinese medicine composition for treating lung cancer comprises the following components in parts by weight: 18 parts of rhizoma paridis, 20 parts of oldenlandia diffusa, 20 parts of raw oyster, 15 parts of rhizoma pinellinae praeparata, 9 parts of thunberg fritillary bulb, 9 parts of snakegourd peel, 9 parts of hairyvein agrimony, 20 parts of coix seed, 9 parts of selfheal, 12 parts of curcuma zedoary, 12 parts of dried orange peel, 12 parts of platycodon grandiflorum, 20 parts of edible tulip, 20 parts of radix pseudostellariae, 18 parts of raw astragalus mongholicus, 9 parts of adenophora tetraphylla and 9 parts of.

Clinical data

1. The general data cases are 2016-2017 and 11 months for outpatient service of specialists in central hospitals in Yichang city. The lung cancer is diagnosed in 21 chest CT and pathological sections, 13 men and 8 women have age of 45-80 years, mean age of 55 years, course of disease of 3 months to 10 years, and mean 2 years.

2. Diagnostic criteria

(1) The chronic choking cough and the chronic dry cough can not be cured for a plurality of weeks, or the chronic dry cough and the chronic dry cough can cause repeated hemoptysis, or the chronic chest pain, the shortness of breath and fever with unknown reasons, or the emaciation and the fatigue.

(2) The age is above 40 years, most men with a long history of smoking.

(3) In the case of chronic cough, when the cough nature is changed, localized inflammation of the lung is repeatedly developed.

(4) Cytological examination of sputum shedding is a simple and effective method for early diagnosis of lung cancer.

(5) Chest X-ray examination, CT, bronchoiography, fiberbronchoscopy.

(6) For patients with long-term smoking, chronic cough, family tumor history, and long-term exposure to certain harmful substances.

(7) For the patient diagnosed with pulmonary tuberculosis, the tuberculosis treatment is ineffective or the focus is improved and a new focus appears after the treatment.

3. The treatment method adopts the traditional Chinese medicine composition of 15g of paris polyphylla, 15g of oldenlandia diffusa, 15g of raw oyster, 10g of rhizoma pinellinae praeparata, 15g of thunberg fritillary bulb, 15g of snakegourd peel, 15g of hairyvein agrimony, 30g of coix seed, 15g of selfheal, 10g of curcuma zedoary, 10g of dried orange peel, 10g of platycodon grandiflorum, 15g of Indian iphigenia bulb, 15g of radix pseudostellariae, 20g of raw astragalus mongholicus, 15g of. One dose is taken after two days, decocted with water and taken orally 3 times a day.

4. The patients pay attention to develop good living habits during the treatment period, and the patients are prevented from high fat diet, giving up smoking and drinking, being forbidden from being overstrain, avoiding mental stress and eliminating worry. 14 days is a treatment course, and the curative effect is observed after 2 to 3 treatment courses.

5. The therapeutic effect is judged by determining the therapeutic index according to the total symptom score before and after treatment and the formula. The curative effect index (the total score of symptoms before treatment-the total score of symptoms after treatment)/the total score of symptoms before treatment x 100%. Efficacy index < 25% is not effective; improvement is carried out when 26% -50%; 51% -85% of the effect is obvious; > 86% is clinically cured.

6. The treatment results of 21 lung cancer patients are counted, after the traditional Chinese medicine is used for treating over 1 month, 14 patients have better cough, hemoptysis, expectoration and chest distress, 12 patients have better general symptoms such as dry mouth, bitter taste, poor appetite, hypodynamia and the like, 14 patients have improved symptoms, and the effective rate is 66.7%. After the traditional Chinese medicine is used for treating more than 6 months, 16 patients have improved cough, hemoptysis, expectoration and chest distress, 15 patients have improved general symptoms such as dry mouth, bitter taste, poor appetite, hypodynamia and the like, and 16 patients have improved symptoms, and the effective rate is 76.2%. After the traditional Chinese medicine is used for treating more than 1 year, 20 patients have improved cough, hemoptysis, expectoration and chest distress, 21 patients have improved general symptoms such as dry mouth, bitter taste, poor appetite, hypodynamia and the like, and 21 patients have improved symptoms, and the effective rate is 100%. 3 cases are cured (14.3%), 12 cases are obviously effective (57.1%), and 6 cases are improved (28.6%). The total effective rate of the traditional Chinese medicine composition reaches 100 percent.

A comparison of patient condition before and after treatment is shown in Table 1.

TABLE 1

The following are typical cases of the present invention:

case one: a woman in Longbei province, 53 years old, will see a doctor in 2016, 8, 9 days, at any time: cough, yellow phlegm, hemoptysis, dry throat, chest distress, dry mouth, bitter taste, poor appetite, pain in ribs, back and head, unsmooth defecation, mental fatigue, dark red tongue with white fur, and wiry and smooth pulse. Clinical diagnosis of left lower lung hypo-differentiated adenocarcinoma IV (phlegm-heat obstructing lung) in 2016 (6 months and 20 days). The targeted therapy is carried out continuously by using medicaments without operations and chemoradiotherapy. After the traditional Chinese medicine decoction is taken for 14 days, cough and expectoration are relieved, hemoptysis occasionally occurs, chest distress is improved, pain of ribs, back and head still exists, and defecation is unsmooth; after the medicine is continuously taken for 30 days, cough and expectoration are obviously improved, chest distress, short breath and hypodynamia are relieved, pain symptoms are relieved, and spirit is improved. After the medicine is taken for 3 months, cough and expectoration occasionally occur, dry mouth and bitter mouth occasionally occur, pains of ribs, back and head are obviously improved, good appetite is achieved, defecation is normal, and sleep is good. The patient continues taking the medicine for 6 months, and the cough, the expectoration and the hemoptysis do not exist, and the pain of ribs, the back and the head occasionally occurs, so that the general symptoms are better.

CT: in 2016, the examination is carried out in the central hospital of Yichang city in 6 months and 15 days, and CT: double lung metastases, liver right lobe metastases, double lung infections. In 2016, 9 and 22 days, the central hospital checks chest X-ray CT (CT890527), the double lungs have the appearance of multiple mass and nodular density-increasing images, the edges are not smooth, burrs can be seen, the sizes are different, the parts are fused, the larger one is positioned in the right upper lung, the diameter is 4.1cm, and multiple thick-wall cavities are formed. The two lungs were seen to have higher density of lamellae, blurred margins, and mild enlargement of mediastinum and lymph nodes. The liver morphology is normal, the right lobe of the liver can see an oval-like low-density focus with a size of 4.2 x 2.6cm, and the boundary is not clear. The liver right lobe lesion was smaller than the anterior panel (2016.08.16). The node image and the cluster image of the double lung are reduced, and the part is obviously reduced and the density is reduced. The range of the double lung sheet-shaped shadow is obviously reduced compared with that of the front sheet. 1. After the right lung cancer and the double lung metastasis treatment, the lung cancer is obviously better than the former 2, the mediastinal and pulmonary lymph nodes are slightly enlarged 3, and the liver right lobe metastasis tumor is smaller than the former. In 2017, day 1 and day 11, chest CT (CT932505) in the central hospital of Yichang city, and the anterior part (2016-09-22) were reviewed, the right upper lung mass was slightly smaller than the anterior part, the maximum cross-sectional area was 25mm 33mm (lung window), and the nodules and the lamellar image of the other two lungs were approximately similar. The low-density foci of the nodules in the lung are smaller than those in the front, the diameter is 32mm, and the mediastinum and the lung portal enlargement lymph nodes are smaller than those in the front.

Case two: a certain ginger, female, 74 years old, will be diagnosed in 2016, 11, 15 days, at the end of the day: cough weakness, yellow and sticky sputum, hemoptysis, sallow and dark face, dizziness and headache, anorexia, dry mouth, insomnia, frequent micturition, red tongue with white and thick coating, and thready pulse. Clinically, lung cancer (deficiency of both qi and yin) is diagnosed in the lower right. The traditional Chinese medicine water decoction is taken for 3 times a day, and 250ml is taken for 1 time, and after the traditional Chinese medicine water decoction is taken for 30 days, cough is weakened and relieved, and hemoptysis happens occasionally; after the medicine is continuously taken for 3 months, the cough and chest distress is obviously relieved, the dizziness and headache are relieved, the appetite is improved, and the sleep quality is improved; after continuous taking for 6 months, the Chinese medicinal composition occasionally has cough, no hemoptysis, no insomnia, bitter taste and dry mouth symptoms, good appetite and improved spirit. The patient is occasionally coughed and has no other obvious symptoms after continuing taking the medicine for about 1 year.

CT: the central hospital in Yichang city of 12 months and 20 days in 2016, coronary bypass and re-examination after left upper lung resection: the left lung volume is reduced, and the upper left shows a wedge-shaped slightly high-density image. The right lung has a plurality of nodules, the texture of the double lungs is increased, the double lungs have nodules and strip-cord density-increasing shadows, and the local pleura is hypertrophic and adhesive; the right lower lung is seen as a thin and blurred image. No obvious enlargement of mediastinal lymph nodes was observed. Partial thoracic vertebrae has uneven bone density. In intrasternal fixation. The anterior panel (2016.3.9) absorbs from the right lower lung foci and the left pleural effusion. 8, 8 and 14 days in 2017, and is applied to the routine CT of the two lungs in the central hospital of Yichang city, after the left lung is cut: the double lung has increased texture, the double lung has a cord-like and nodular high-density image, and no obvious swollen lymph node image is seen at mediastinum. The bone density of partial vertebral body and left rib is insufficient. The anterior segment (2017.6.21) is slightly smaller in the left lower lung nodule than in the anterior segment, and slightly absorbed in the right lower lung macula, the remainder being approximately the same.

Case three: su somewhere, man, 65 years old, visit 16 days 8 months in 2016, at the moment: cough, thick expectoration, short breath, asthenia, chest distress, skin pruritus, poor appetite, femur pain, red tongue with little coating, and thready pulse. Clinical diagnosis of upper right squamous cell carcinoma at the 4-month T2N0M0 IIa stage (deficiency of both qi and yin). The traditional Chinese medicine water decoction is taken for 3 times and 1 time, 250ml, and after the decoction is taken for 10 days, cough and expectoration are relieved, and chest distress is improved. After 30 days of administration, occasionally coughing and chest distress, no air short and hypodynamia, skin pruritus improvement and occasional femoral pain. After taking the medicine for 3 months, the patient has no cough, good appetite and no obvious general symptoms. After the medicine is taken for 6 months, the patient can self-state without obvious symptoms.

CT, 2016, 9, 12 months, hospitalization: 1. after the operation of the right upper lung and the operation of the right rib, the right pleural effusion is partially wrapped, 2 chronic bronchitis and emphysema are reduced compared with the prior art, and the old pulmonary tuberculosis of double lungs, the fibroproliferative focus of double lungs, the calcific focus 4 and the thick and adherent pleura on both sides are considered to be accompanied with calcification. Chest CT is checked in the central hospital in 2 month and 17 days in 2017: after the right upper lung resection, the double lung has increased and disordered textures, and the right lung portal structure is less clear, and the inside of the lung portal structure is calcified. The two lungs were seen with multiple nodules and increased density of strips, some with calcification and unclear borders. Hypertrophic and adherent pleura on both sides with calcification. There were more mediastinal lymph nodes, and some were mildly enlarged. The right thoracic cavity was partially covered by a low density liquid zone. Right multiple rib postoperative change. The right pleural effusion was slightly reduced compared to the anterior case (2016-09-12).

Case four: wu Yi, male, 49 years old, in 2017, on 17 months, at the end of the treatment: frequent cough, yellow and sticky sputum, hemoptysis, dry mouth, chest distress and hypodynamia, insomnia, frequent urination, dry stool, fever and night sweat, red tongue with white and thick coating and thready and rapid pulse. Clinical diagnosis left upper lung squamous carcinoma operation for more than 2 years, and postoperative recurrence of pT2N2M1 in IV stage (deficiency of both qi and yin). The traditional Chinese medicine water decoction is taken for 3 times and 1 time, 250ml, and after the traditional Chinese medicine water decoction is taken for 14 days, cough is relieved, hemoptysis occasionally occurs, chest distress is improved, night sweat, insomnia and dry stool are still caused; after the medicine is continuously taken for 30 days, the cough and hemoptysis are obviously relieved, chest distress and hypodynamia are occasionally caused, the sleep quality is better, and the defecation is normal; after continuous taking for 3 months, the cough-relieving and blood-stasis-relieving tea has the advantages of occasional cough, no hemoptysis, no chest distress, hypodynamia, night sweat, good appetite, good night sleep and good spirit. After the medicine is taken for 6 months, the patient has no obvious subjective symptoms.

CT: examination in the central hospital of Yichang city in 3 and 16 days 2017, chest CT (957637): after left lung operation, the tracheal mediastinum is moved to the left, and a small amount of pleural effusion is left. The right lung showed a few small nodule shadows and the lymph nodes under the carina bronchi were slightly enlarged. The right main bronchus is partially narrowed, and the lumen is not smooth. No obvious abnormal enhancement focus is seen in both lungs of the enhanced scan. The shadow of the lobular macula in the right lung is basically absorbed by the anterior segment (2017-01-23). 1. After left lung surgery, a small amount of fluid in the left pleural cavity is associated with pachynsis pleura. Little fibroplasia focus on right lung. 2. The lower lymph node of the carina process is slightly enlarged, the local lumen of the right main bronchus is slightly narrowed 3, and multiple nodules are generated on the right lung. Examination in the central hospital of Yichang city in 2017, 5 and 11 months, chest CT (983212): after left lung operation, the tracheal mediastinum is moved to the left, and a small amount of pleural effusion is left. A few small nodule images appear in the right lung, and small spot-shaped blurred images appear in the right lower lung. The lower lymph node of the carina bronchiorum was slightly enlarged. The lower right lung lobe patch image is slightly larger than the frontal patch (2017.03.16). In 2017, 6 and 8 days, the patient is suitable for chest CT (995705) examination in the central hospital of Yichang city, after left lung operation, the trachea mediastinum moves left, and a small amount of effusion is accumulated in the left chest. A few nodule images appear in the right lung, and small-spot flaky fuzzy images appear in the right lower lung. The lower lymph node of the carina bronchiorum was slightly enlarged. The anterior panels (2017-05-11), are substantially similar. Conventional CT for breast in hospital in center of 8 months and 10 days in 2017 (CT 1025998): after left lung operation, the tracheal mediastinum is moved to the left, and a small amount of pleural effusion is left. A few small nodule images appear in the right lung, and small spot-shaped blurred images appear in the right lower lung. The lower lymph node of the carina bronchiorum was slightly enlarged. The shadow of the lung spot on the lower right side is slightly absorbed compared with the shadow of the front panel (2017-06-08), and the rest is approximately the same. Chest CT is examined in the central hospital of Yichang city of 10 months and 23 days in 2017: after left lung operation, the tracheal mediastinum is moved to the left, and a small amount of pleural effusion is left. The right lung has a few nodules and the right lower lung has a few striae. The lower lymph node of the carina bronchiorum was slightly enlarged. The front panel (2017-08-10), the right lower lung, blurred image, is mostly absorbed, and the rest are approximately the same.

Experimental study

Experimental study on inhibition effect of traditional Chinese medicine composition on human lung cancer A549 and H1299 cells

The experimental part firstly screens out 17 medicines with corresponding effects and obvious effects as factors through clinical experience medication, and determines the effect of inhibiting the lung cancer by using the composition of the invention for inhibiting the growth and migration of lung cancer cells and inducing the morphological change of the lung cancer cells as indexes.

1. Experimental Material

1.1 Experimental materials and reagents radix Glehniae, radix Adenophorae, radix Pseudostellariae, pericarpium Citri Tangerinae, rhizoma Pinelliae Preparata, radix astragali, rhizoma paridis, herba et Gemma Agrimoniae, Prunellae Spica, herba Hedyotidis Diffusae, Pseudobulbus Cremastrae seu pleiones, radix Platycodi, Concha Ostreae, Coicis semen, Bulbus Fritillariae Thunbergii, Curcumae rhizoma, and pericarpium Trichosanthis are provided by people hospitals in Yichang city. RPMI1640 medium, fetal bovine serum, diabody, phosphate buffered saline PBS, trypsin (Gibco); DMSO (dimethyl sulfoxide, shanghai bio-engineering ltd); sexazole blue (MTT, Sigma, batch No. M2128).

1.2 preservation of human Lung cancer cells A549 and H1299 in the third-class research laboratory

Evaporator RE-52A (Shanghai Yangrong Biochemical apparatus factory); KQ-250B ultrasonic cleaner (Kunshan ultrasonic instruments Co., Ltd.); KDM type thermoregulation electric heating jacket (shandong Juancheng permanent light instrument factory); chinese herbal medicine grinder (China); DK-S26 electric heating constant temperature water bath (Shanghai sperm macro experimental facilities Co., Ltd.); a microsampler (200. mu.L, one each 1000. mu.L); optical microscope, Olympus, japan; the model is as follows: XS-213; full wavelength microplate reader, Thermo corporation, model: mutiskan Spectrum; CO2 constant temperature cell culture chamber, SANYO corporation, japan, model: MCO175

2. Experimental methods

2.1 the Chinese medicinal composition is selected

Selection criteria: the clinical application proves that the traditional Chinese medicine composition is effective in treating lung cancer.

Selecting the following medicines: 15g of radix glehniae, 15g of radix adenophorae, 15g of radix pseudostellariae, 10g of dried orange peel, 10g of rhizoma pinellinae praeparata, 15g of radix astragali, 15g of rhizoma paridis, 15g of hairyvein agrimonia herb and bud, 15g of selfheal, 15g of oldenlandia diffusa, 15g of edible tulip, 10g of platycodon grandiflorum, 10g of raw oyster, 30g of semen coicis, 15g of thunberg fritillary bulb, 10g of rhizoma zedoariae and 15 g.

2.2 the traditional Chinese medicine composition is extracted: putting the traditional Chinese medicine composition into a round-bottom flask, adding water to submerge medicinal materials, soaking for half an hour, putting the round-bottom flask into a heating sleeve, and installing devices such as a condensation pipe and the like for heating. Decocting with slow fire, adjusting to slow fire when water is boiling, decocting for half an hour, filtering to obtain medicinal liquid, repeating above steps for 2 times, and mixing the obtained medicinal liquids. Filtering the obtained medicinal liquid for 1-2 times. Evaporating the filtered medicinal liquid in a water bath kettle at 80 deg.C until the liquid is concentrated to 100ml, freeze drying 50ml, weighing 22.57g, and preparing into medicinal culture solution containing 10% fetal calf serum 450 mg/ml. Before administration, the preparation is diluted with culture medium, filtered and sterilized with 0.22um microporous membrane, and then used.

2.3MTT method for determining influence of A549 and H1299 medicines in cell proliferation experiment

Taking A549 and H1299 cells in logarithmic growth phase, digesting with pancreatin to prepare cell suspension, counting cells and adjusting cell concentration to 4 x 10⁴Per mL; the cell suspension was inoculated into a 96-well plate (100. mu.L/well) using a pipette, and then the 96-well plate was placed at 37 ℃ with 5% CO²The cell culture box is used for culturing and incubating for 24 hours; adding 100 μ L of the medicine into each well, setting a blank group, a normal control group and a Chinese medicinal composition group with different concentrations, setting 5 multiple wells for each concentration, and culturing for 24h and 48h respectively; sucking out the medicine, adding PBS for washing, adding 100ul of MTT solution (MTT: serum-free medium diluted 1:9) into each hole, and placing the culture plate in a cell culture box for further incubation for 4 hours; the absorbance value (OD value) of each well at a wavelength of 570nm was measured with a microplate reader, and the cell growth inhibition (%) was calculated as (control OD value-experimental OD value)/(control OD value-blank OD value) × 100% according to the following formula.

2.4 cell migration assay

Drawing transverse lines along the straight ruler on the back of the 6-hole plate by using a Marker pen, enabling the transverse lines to pass through the holes, enabling the interval between the lines to be 0.5cm, and drawing 5 parallel transverse lines in each hole; preparing cell suspension from A549 and H1299 cells in logarithmic growth phase, counting cells, and adjusting cell concentration to 5 × 10⁵Per mL; adding the cell suspension into a 6-well plate according to 2 mL/well, culturing for a proper time, scratching the cell suspension along a transverse line which is perpendicular to the back of the culture plate and is perpendicular to a ruler by using a 10-microliter gun head after the cell is paved in the 6-well plate for about 90% of the density, and slightly washing the cell suspension for 2 times by using PBS (phosphate buffer solution) to remove the scratched cell; serum-free medium was then added to the 6-well plate to set the normal groupThe control group (DDP1 mu g/ml) and the medicine groups with different concentrations are taken under an inverted microscope, pictures of the scratched area of the cells of each group are taken when the time is 0h, the corresponding medicines are added according to different groups, the medicines are continuously cultured in an incubator containing 5% CO2 at 37 ℃ for 24h and 48h, the pictures of the scratched area of the cells are taken under the inverted microscope respectively, and the experiment is repeated for 3 times.

2.5 morphological experiments

Taking human lung cancer cells A549 and H1299 in logarithmic phase, carrying out trypsinization, then carrying out heavy suspension on the cells by using a complete culture medium, inoculating the heavy suspension cells to a disposable cell culture dish, dividing the disposable cell culture dish into a traditional Chinese medicine composition and a normal control group, respectively adding 0.5mg/ml of traditional Chinese medicine composition preparation liquid and an equal amount of culture medium into the cell culture liquid, placing the culture dish into a cell culture box for culturing for 24 hours, and observing morphological changes of the cells under different magnifications by using an inverted microscope.

2.6 statistical processing of data

Statistical analysis was performed using Spss18.0 statistical software. The data are expressed by mean standard deviation (+ -s), two groups are compared, and when the variances are uniform, the LSD method is used for analyzing, and when the variances are not uniform, the Tamhane method is used for analyzing. p <0.05 was considered statistically significant and p <0.01 was considered statistically significant.

3. Results

3.1 the inhibition rate of the extract of the traditional Chinese medicine composition on the proliferation of A549 and H1299 cells

As shown in Table 2 and FIG. 1, the MTT method was used to determine the cell inhibition rate of the extract of Chinese medicinal composition at different concentrations at two time points of 24h and 48 h. The results show that different drug concentrations show different inhibition effects on the growth of human lung cancer cell A549 cells at different time points. Wherein, the inhibition rate is obviously improved along with the increase of the concentration, and compared with a normal group, the difference has obvious statistical significance (P is less than 0.01). And under the same concentration, the inhibition rate of 48h is obviously higher than 24h (P < 0.05). When the concentration of the Chinese medicinal composition extracting solution is 1mg/ml, the inhibition rate of the Chinese medicinal composition extracting solution reaches 84.64% after 48 hours.

As shown in Table 3 and FIG. 2, the MTT method was used to determine the H1299 cell inhibition rate of the extracts of the Chinese medicinal composition with different concentrations at two time points of 24H and 48H. The results show that different drug concentrations show different inhibition effects on the growth of H1299 cells at different time points, and the inhibition rate is increased along with the increase of the concentration, and compared with a normal group, the inhibition rate is remarkably and statistically different (P is less than 0.01). Under the same concentration, the difference between the 48h inhibition rate and the 24h inhibition rate has no obvious statistical significance. When the concentration of the Chinese medicinal composition extracting solution is 0.5mg/ml, the inhibition rate of the Chinese medicinal composition extracting solution reaches 66.86% after 48 hours.

TABLE 2 inhibition ratio of Chinese medicinal composition with different concentrations on the growth of A549 cells in 24h and 48h

Note that in-group comparison, comparison with the OD value of the normal group^*P＜0.05，^**P is less than 0.01; OD value comparison of

same concentration

24h and 48h^#P＜0.05，^##P＜0.01。

TABLE 3 inhibition rate of different concentrations of Chinese medicinal composition on growth of H1299 cells for 24H and 48H

Note that in-group comparison, comparison with the OD value of the normal group^*P＜0.05，^**P is less than 0.01; comparison between groups^#P＜0.05；^##P＜0.01。

3.2 the influence of the extract of the Chinese medicinal composition on the migration ability of the lung cancer cells A549 and H1299

According to the MTT experiment result, the traditional Chinese medicine composition of 0.5mg/ml and 1mg/ml is selected to treat A549 cells and H1299 cells, and the group is divided into a normal group, a low-dosage group of the traditional Chinese medicine composition, a high-dosage group of the traditional Chinese medicine composition and a control group (DDP1 mu g/ml). The change of the scarred areas of the human lung cancer cells A549 and H1299 within 24H and 48H is observed by using an inverted microscope. The results show that: compared with the blank control group, the scratch area of the blank control group has a large amount of cell re-migration coverage, and compared with the blank control group, the scratch area of the low-dose and high-dose groups of the traditional Chinese medicine composition has a small amount of cell re-migration coverage, but the number of the migrated cells is obviously reduced, and the area re-covered by the cells is not obvious, so that the traditional Chinese medicine composition can inhibit the migration of A549 cells and H1299 cells, and the results have significance, and the results are shown in figure 3 and figure 4.

3.4 the influence of the extract of the Chinese medicinal composition on the morphology of the lung cancer cells A549 and H1299

As shown in fig. 5 and fig. 6, the cells in the normal control group are arranged regularly, tightly connected, clear in cell morphology and cell membrane boundary, and the nucleus is circular and located in the center of the cell; after 1mg/ml of the traditional Chinese medicine composition is used for acting, the number of cells is obviously reduced, the cell spacing is increased, the shape is irregular, the internal particles are increased, part of the cells become round and shrink, and the dead cells are dropped off, so that the cells are gradually crushed and die.

4. Discussion of the related Art

The lung cancer has high morbidity and mortality, poor prognosis and easy metastasis and relapse. The existing treatment means comprises surgery and radiotherapy and chemotherapy, and is accompanied by serious adverse reaction and poor prognosis. The traditional Chinese medicine for treating lung cancer is characterized by controlling the disease condition, enabling a patient to live with tumor for a long time, improving the life quality and prolonging the life cycle.

According to the etiology analysis of lung cancer, the traditional Chinese medicine considers that the lung cancer is caused by the deficiency of vital qi and the invasion of pathogenic toxin. In the aspect of treatment, all doctors have different summaries, and the traditional Chinese medicine theory and the modern research of traditional Chinese medicine provide a lot of microscopic data for us to become the basis for selecting the prescription. Clinical effective Chinese medicines are selected for experimental research, and 17 Chinese medicines are preliminarily determined to have the effects of clearing away heat and toxic materials, eliminating stagnation and reducing swelling, tonifying vital qi, promoting blood circulation to remove blood stasis and the like.

The clinical research on 21 patients proves that after the traditional Chinese medicine composition is used for treating all patients for 14 days, the body function, the emotional function, the general health condition, the symptoms of loss of appetite, shortness of breath, cough and chest distress are obviously improved, and the effective rate reaches 100%. Wherein, CT in typical cases shows that the lesion is obviously absorbed and shrunk before the lesion, and the lung cancer marker also obviously tends to be normal.

Modern research shows that the occurrence of a plurality of diseases is closely related to the process of apoptosis, so that the apoptosis has important significance in the evaluation of anti-tumor drugs. Apoptosis is a process of cell death actively involved in gene-encoded programs after cells are stimulated by certain signals in certain physiological or pathological conditions. Apoptosis is an active death process caused by the triggering of the death cascade in cells under the regulation and control of related genes in order to keep the stability of the body tissues and keep the balance between the proliferation and the death of the cells. Research shows that many anti-tumor traditional Chinese medicines can finally trigger tumor cell apoptosis to achieve the purpose of treating tumors by interfering the processes of tumor cell growth, metabolism, proliferation and the like, so that the cell apoptosis also becomes a new index for evaluating the curative effect.

The experiment is to use the human lung cancer cells A549 and H1299 to culture in vitro and explore the influence of the traditional Chinese medicine composition on the apoptosis of the lung cancer cells. Human lung cancer cells A549 and H1299 are selected. Stored in the third-level laboratory of this school. Cell culture is performed strictly aseptically.

The in vitro research of different lung cancer cells by different mass concentrations of the traditional Chinese medicine composition is applied to the experimental research. MTT experiment results show that the traditional Chinese medicine can obviously inhibit the growth of lung cancer cells and is dose-dependent. The higher the concentration, the higher the inhibition rate on the cells. When the concentration is 0.5mg/ml, the inhibition rate of H1299 cells for 24H reaches 55.83 percent. When the concentration is 1mg/ml, the 24h inhibition rate of the A549 cells reaches 50 percent. In addition, for cell migration experiments, the traditional Chinese medicine composition can inhibit the migration of A549 cells and H1299 cells when the concentration is 0.5 mg/ml. Morphological observation is the most intuitive method for judging apoptosis. The cell apoptosis can be judged visually and simply by observing the morphological characteristics of the cells under a light mirror. Apoptotic cells will undergo characteristic changes in morphology: firstly, the cells are shrunk, the cell density is increased, then the chromatin is highly condensed due to the disintegration of the nuclear fiber layer and the cytoskeleton, the condensed chromatin can surround the periphery of the nucleus (called edge aggregation) or form a crescent structure, and the condensed nucleus is often cracked into a plurality of large fragments and is dispersed in different parts of the cells; the cells are then lysed into several apoptotic bodies by means of cell membrane invagination or foaming, budding, etc. The cells are observed under the light microscope for fine shrinkage, the chromatin is highly condensed, and then the cells are cracked and killed.

The generation and development of lung cancer are the change process of multiple factors, multiple stages and multiple sites, the generated pathology and molecular mechanism are complex, and the traditional Chinese medicine correspondingly has the characteristics of multiple components, multiple systems and multiple target sites for comprehensive anti-liver tumor effect.

Claims

1. The traditional Chinese medicine composition for treating lung cancer is characterized by being prepared from the following components in parts by weight: 15-20 parts of rhizoma paridis, 15-20 parts of oldenlandia diffusa, 15-20 parts of raw oyster, 9-15 parts of rhizoma pinellinae praeparata, 9-15 parts of thunberg fritillary bulb, 9-15 parts of pericarpium trichosanthis, 9-15 parts of hairyvein agrimony, 15-30 parts of semen coicis, 9-15 parts of selfheal, 9-12 parts of curcuma zedoary, 9-12 parts of pericarpium citri reticulatae, 9-12 parts of platycodon grandiflorum, 15-20 parts of Indian iphigenia bulb, 15-20 parts of radix pseudostellariae, 9-15 parts of adenophora tetraphylla, 9-15 parts of radix glehniae.

2. The traditional Chinese medicine composition according to claim 1, characterized in that: 15 parts of rhizoma paridis, 15 parts of oldenlandia diffusa, 15 parts of raw oyster, 10 parts of rhizoma pinellinae praeparata, 15 parts of thunberg fritillary bulb, 15 parts of snakegourd peel, 15 parts of hairyvein agrimony, 30 parts of coix seed, 15 parts of selfheal, 10 parts of curcuma zedoary, 10 parts of dried orange peel, 10 parts of platycodon grandiflorum, 15 parts of edible tulip, 15 parts of radix pseudostellariae, 15 parts of raw astragalus membranaceus, 15 parts of adenophora tetraphylla and 15 parts of.

3. The traditional Chinese medicine composition according to claim 1 or 2, wherein: the Chinese medicinal composition is prepared into liquid medicine, granules, plaster or other pharmaceutically acceptable dosage forms after extracting effective substances from the raw material components.

4. A process for preparing a Chinese medicinal composition as claimed in claim 1 or 2, comprising the steps of: mixing all the raw materials in proportion, adding water to submerge the medicinal materials, decocting with slow fire until the water is boiled, decocting for 20 minutes, filtering to obtain medicinal juice, repeating the decocting and filtering processes twice, and mixing the obtained medicinal juice to obtain the decoction of the traditional Chinese medicine composition for treating lung cancer.

5. Use of the Chinese medicinal composition of any one of claims 1-3 or prepared by the method of claim 4 in the preparation of a medicament for the treatment of lung cancer.