CN108434115A - Anti-fungal infection allicin effervescent tablet and its preparation method and application - Google Patents

Anti-fungal infection allicin effervescent tablet and its preparation method and application Download PDF

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Publication number
CN108434115A
CN108434115A CN201810196522.1A CN201810196522A CN108434115A CN 108434115 A CN108434115 A CN 108434115A CN 201810196522 A CN201810196522 A CN 201810196522A CN 108434115 A CN108434115 A CN 108434115A
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China
Prior art keywords
allicin
fungal infection
effervescent tablet
desired amount
mesh screen
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CN201810196522.1A
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Chinese (zh)
Inventor
陈尚珂
邓丽娟
黄琼
鲁婷
胡小霞
郭丹
杨小刚
李阿萍
艾波
王涛
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XINJIANG AILEXIN PHARMACEUTICAL CO Ltd
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XINJIANG AILEXIN PHARMACEUTICAL CO Ltd
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Priority to CN201810196522.1A priority Critical patent/CN108434115A/en
Publication of CN108434115A publication Critical patent/CN108434115A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
    • A61K31/198—Alpha-aminoacids, e.g. alanine, edetic acids [EDTA]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
    • A61K31/255—Esters, e.g. nitroglycerine, selenocyanates of sulfoxy acids or sulfur analogues thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0002—Galenical forms characterised by the drug release technique; Application systems commanded by energy
    • A61K9/0007—Effervescent
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/2027—Organic macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2095—Tabletting processes; Dosage units made by direct compression of powders or specially processed granules, by eliminating solvents, by melt-extrusion, by injection molding, by 3D printing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/02—Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics

Abstract

The present invention relates to allicin technical fields, it is a kind of anti-fungal infection allicin effervescent tablet and its preparation method and application, the anti-fungal infection allicin effervescent tablet, raw material includes garlic powder or one kind in allicin composition, soda acid disintegrant, filler, binder, lubricant, draws humectant, allicin composition includes alliin and allinase, and soda acid disintegrant includes acid source and alkali source.The present invention is using allicin composition or garlic powder as raw material, it is mixed and made into allicin effervescent tablet with acid source, alkali source and other auxiliary materials, its raw material is pure natural garlic extract, it has no toxic side effect, there is strong killing inhibiting effect to pathomycete and its drug-fast bacteria, both can external application, also oral administration, the used time is also easy to store and transport.Anti-fungal infection allicin effervescent tablet of the present invention has the characteristics that quick, efficient, stable anti-fungal infection, is applied to the therapeutic effect of gynecological infection disease more preferably especially as vagina effervescence.

Description

Anti-fungal infection allicin effervescent tablet and its preparation method and application
Technical field
It is a kind of anti-fungal infection allicin effervescent tablet and preparation method thereof the present invention relates to allicin technical field And application.
Background technology
Fungi is the enkaryotic microorganism that a major class has typical cells core, and fungal infection is more normal in dept. of dermatology See, can cause superficial mycoses and deep mycosis after clinically infecting fungi.Mycotic infection of superficial part be mainly dermatophyte such as Epidermophyton, trichophyta, sporidiole bacteria invade the keratinized tissues such as skin, hair, nail and cause favus of the scalp, tinea pedis, refer to tinea and ringworm of the body Deng；Deep fungal infection refer to by the fungies such as cryptococcus, Aspergillus invade internal organ (such as lung, brain, alimentary canal organ), subcutaneous group It knits, below keratoderma and caused by mucous membrane, severe patient can cause endocarditis, meningitis and septicemia, harmfulness larger.Mesh The antifungal drug of preceding clinical application is mainly azole antibiotic, however drug resistance, natural drug resistance easily occurs very much in such antibiotic Strain is continuously increased, therefore is found the efficient antifungal drug of low toxicity from natural drug and just become more important.
Garlic (Allium sativum L.) is the generally acknowledged medical and edible dual purpose plant of different parts of the world, different nationalities.China Ancient times《Tang materia medica》(Tang),《Compendium of Materia Medica》(bright) record garlic has：Exorcise evil spirits evil, subduing inflammation, only cholera, and evil removing is haunt and plague, solution The effect of pestilence, goes disease due to noxious agents produced by various parasites, malignant sore, snakeworm, malicious small stream.It is anti-a variety of pathogenic thin that recent studies shows that garlic and its extract have Bacterium, fungi, virus and protozoon activity.Effective antibacterial composition of garlic is allicin, by garlic bulb intracellular reactive The catalysis of substance allinase is split conjunction alliin and is formed.Allicin, chemical name：Diallyl thiosulfinates (Diallylthiosulfinate, 2-propene-1-sulfinothioic acid S-2-propenyl ester), slightly soluble Yu Shui, under room temperature in air, it is unstable to meet light, heat or organic solvent, is easily degraded into a variety of organic compounds containing sulfurs.For gram This unstable problem of the anti-infective active ingredient of garlic is taken, our company is according to independent research patented technology：Application No. is 03100420.2 " from alliin production technology is extracted in fresh garlic " and " from fresh garlic extract garlic application No. is 03100419.9 Enzyme production technology " extracts the metastable high-content alliin of chemical property and high vigor allinase raw material from fresh garlic respectively, then Binary drug release preparation is made in the two, reaches the anti-infectious purpose of efficient stable.
Effervescent tablet means containing sodium bicarbonate and organic acid, and gas can be generated and the tablet in effervesce shape by meeting water, had and protected Deposit easy to carry, disintegration rate is fast, rapid-action, and bioavilability is high, it is water-soluble it is for oral administration also can external application, also can be done directly on Local patholoic change position is used for the prevention of gynaecologic vaginal disease.
Invention content
The present invention provides a kind of anti-fungal infection allicin effervescent tablets and its preparation method and application, overcome above-mentioned The deficiency of the prior art, capable of effectively solving existing garlic, antibacterial composition allicin is unstable, it is slow to work, controls in the presence of effectively resisting Treatment effect not enough and only oral administration the problem of.
One of technical scheme of the present invention is realized by following measures：A kind of anti-fungal infection allicin effervesce Piece, it includes one kind in 1 part to 20 parts of garlic powder and allicin composition, 25 parts of soda acid disintegrant that raw material is counted in parts by weight To 50 parts, 25 parts to 45 parts of filler, 1 part to 5 parts of binder, 1 part to 5 parts of lubricant, draw 1 part to 3 parts of humectant, wherein press Parts by weight meter allicin composition includes 0.1 part to 5 parts of alliin, 0.1 part to 5 parts of allinase, and soda acid disintegrant includes acid Source and alkali source.
Here is the further optimization and/or improvements to foregoing invention technical solution：
Above-mentioned acid source is one or more of citric acid, boric acid, tartaric acid, malic acid, and alkali source is sodium bicarbonate, bicarbonate One or more of potassium, sodium carbonate；Or/and filler is one kind in microcrystalline cellulose, dextrin, lactose, mannitol, sucrose More than；Or/and binder be hydroxypropyl methylcellulose aqueous solution, polyvinylpyrrolidone ethanol solution, olefin(e) acid resin aqueous solution, Starch slurry, syrup, one or more of the ethanol solution that concentration of volume percent is 95%；Or/and lubricant is benzoic acid In sodium, enuatrol, sodium chloride, sodium acetate, sldium lauryl sulfate, L-Leu, Macrogol 4000 and Macrogol 6000 A kind of one or more of, magnesium stearate；Or/and it is sodium chloride to draw humectant.
Above-mentioned acid source is citric acid or tartaric acid, and alkali source is sodium bicarbonate, and the mass ratio of acid source and alkali source is 5 to 7：5 to 7；Or/and binder is polyvinylpyrrolidone ethanol solution；Or/and lubricant is in Macrogol 6000 and magnesium stearate More than one.
Above-mentioned to be prepared using full pelletizing press sheet method, full pelletizing press sheet method carries out as steps described below：The first step, The desired amount of allicin composition or garlic powder are weighed, is uniformly mixed, obtains with the desired amount of acid source, filler, binder Softwood material sieving mesh screen point is obtained wet grain material, third step, by wet grain material at 20 DEG C to 40 by softwood material, second step Under the conditions of DEG C, after dry 2h to 5h, sieving mesh screen point obtains dry granular material, the 4th step, by dry granular material and the desired amount of alkali Source, lubricant, draw humectant be uniformly mixed, tabletting obtains anti-fungal infection allicin effervescent tablet；Alternatively, using half granulation pressure Piece method is prepared, and half granulation tabletting carries out as steps described below：The first step, weigh the desired amount of allicin composition or Garlic powder is uniformly mixed with the desired amount of acid source, filler, binder, obtains softwood material, second step, by softwood material mistake Sieve sieves, and obtains wet grain material, and third step after dry 2h to 5h, crosses sieve by wet grain material under the conditions of 20 DEG C to 40 DEG C Screening, obtains dry granular material, the 4th step, by dry granular material and the desired amount of alkali source, lubricant, draws humectant and is uniformly mixed, tabletting, Obtain anti-fungal infection allicin effervescent tablet；Alternatively, being prepared using pelletizing press sheet method is separated, separates pelletizing press sheet and press It is carried out according to following step：The first step weighs the desired amount of allicin composition or garlic powder, with the desired amount of acid source, filling Agent, binder are uniformly mixed, and obtain softwood material one, and the sieving mesh screen point of softwood material one is obtained wet grain material by second step One, third step, by wet grain material one under the conditions of 20 DEG C to 40 DEG C, after dry 2h to 5h, sieving mesh screen point obtains dry granular material One, the 4th step, then the desired amount of allicin composition or garlic powder are weighed, it is mixed with the desired amount of alkali source, filler, binder It closes uniformly, obtains softwood material two, by the sieving mesh screen point of softwood material two, wet grain material two is obtained, by wet grain material two 20 DEG C under the conditions of 40 DEG C, after dry 2h to 5h, sieving mesh screen point obtains dry granular material two, the 5th step, by dry granular material one, dry Grain material two, with the desired amount of lubricant, draw humectant and be uniformly mixed, tabletting obtains anti-fungal infection allicin effervescent tablet.
Above-mentioned garlic powder contains alliin and allinase, wherein alliin weight content is 3% to 99%；Or/and garlic ammonia Sour purity is more than or equal to 50%, and allinase vigor is more than or equal to 1000U/g.
Above-mentioned sieving mesh screen timesharing, sieve are 20 mesh to 40 mesh.
Technical scheme of the present invention second is that being realized by following measures：A kind of anti-fungal infection allicin effervesce Preparation method, carried out using full pelletizing press sheet method, full pelletizing press sheet method carries out as steps described below：The first step weighs The desired amount of allicin composition or garlic powder, are uniformly mixed with the desired amount of acid source, filler, binder, obtain softwood Softwood material sieving mesh screen point is obtained wet grain material, third step, by wet grain material in 20 DEG C to 40 DEG C items by material, second step Under part, after dry 2h to 5h, sieving mesh screen point obtains dry granular material, the 4th step, by dry granular material and the desired amount of alkali source, profit Lubrication prescription, draw humectant be uniformly mixed, tabletting obtains anti-fungal infection allicin effervescent tablet；Alternatively, using half granulation tabletting method It carries out, half granulation tabletting carries out as steps described below：The first step weighs the desired amount of allicin composition or garlic powder, with The desired amount of acid source, filler, binder are uniformly mixed, and obtain softwood material, and second step divides softwood material sieving mesh screen, Wet grain material is obtained, third step, by wet grain material under the conditions of 20 DEG C to 40 DEG C, after dry 2h to 5h, sieving mesh screen point obtains Dry granular material, the 4th step by dry granular material and the desired amount of alkali source, lubricant, are drawn humectant and are uniformly mixed, and tabletting obtains anti-true Bacterium infects allicin effervescent tablet；Alternatively, carried out using pelletizing press sheet method is separated, separate pelletizing press sheet as steps described below into Row：The first step weighs the desired amount of allicin composition or garlic powder, is mixed with the desired amount of acid source, filler, binder Uniformly, softwood material one is obtained, the sieving mesh screen point of softwood material one is obtained wet grain material one, third step will be wet by second step Grain material one is under the conditions of 20 DEG C to 40 DEG C, and after dry 2h to 5h, sieving mesh screen point obtains dry granular material one, the 4th step, then claim The desired amount of allicin composition or garlic powder are taken, is uniformly mixed with the desired amount of alkali source, filler, binder, is obtained soft The sieving mesh screen point of softwood material two is obtained wet grain material two, by wet grain material two in 20 DEG C to 40 DEG C conditions by material material two Under, after dry 2h to 5h, sieving mesh screen point obtains dry granular material two, the 5th step, by dry granular material one, dry granular material two and institute The lubricant of requirement, draw humectant be uniformly mixed, tabletting obtains anti-fungal infection allicin effervescent tablet.
Here is two further optimization and/or improvements to foregoing invention technical solution：
Above-mentioned garlic powder contains alliin and allinase, wherein alliin weight content is 3% to 99%；Or/and garlic ammonia Sour purity is more than or equal to 50%, and allinase vigor is more than or equal to 1000U/g.
Above-mentioned sieving mesh screen timesharing, sieve are 20 mesh to 40 mesh.
The three of technical scheme of the present invention are realized by following measures：A kind of anti-fungal infection allicin effervesce Application of the piece in preparing anti-fungal infection or/and sterilizing effervescent tablet.
The present invention mixes system using allicin composition or garlic powder as raw material, with acid source, alkali source and other auxiliary materials At allicin effervescent tablet, raw material is pure natural garlic extract, has no toxic side effect, has by force to pathomycete and its drug-fast bacteria Strong killing inhibiting effect, both can external application, also oral administration, the used time is also easy to store and transport.Anti-fungal infection garlic of the present invention is peppery Plain effervescent tablet has the characteristics that quick, efficient, stable anti-fungal infection, is applied to gynecological infection disease especially as vagina effervescence The therapeutic effect of disease is more preferably.
Specific implementation mode
The present invention is not limited by following embodiments, can be determined according to the technique and scheme of the present invention with actual conditions specific Embodiment.It is previously mentioned various chemical reagent and chemical article in the present invention unless otherwise specified, is public in the prior art Know common chemical reagent and chemical article；Percentage in the present invention is mass percent as not having specified otherwise；This hair It is the aqueous solution that solvent is water, for example, hydrochloric acid solution is aqueous hydrochloric acid solution if the solution in bright is without specified otherwise；This Room temperature, room temperature in invention refer generally to 15 DEG C to 25 DEG C of temperature, are commonly defined as 25 DEG C.
With reference to embodiment, the invention will be further described：
Embodiment 1：The anti-fungal infection allicin effervescent tablet, raw material is counted in parts by weight includes garlic powder 1 part to 20 Part or one kind in allicin composition, 25 parts to 50 parts of soda acid disintegrant, 25 parts to 45 parts of filler, 1 part to 5 of binder Part, draws 1 part to 3 parts of humectant at 1 part to 5 parts of lubricant, wherein it includes alliin 0.1 to count allicin composition in parts by weight For part to 5 parts, 0.1 part to 5 parts of allinase, soda acid disintegrant includes acid source and alkali source.
Garlic powder is obtained according to conventionally known technology, i.e. garlic is after dividing valve, decortication, cleaning, sterilizing, slice, drying Or obtain garlic powder after freeze-drying, crushing.
Allicin composition includes alliin and allinase, and alliin is existing commercially available or by known application It number is prepared for 03100420.2 " from fresh garlic extract alliin production technology " patented technology；Allinase is purchased for existing business It buys or is prepared by known " extracting allinase production technology from fresh garlic " patented technology application No. is 03100419.9 It arrives.
Embodiment 2：The anti-fungal infection allicin effervescent tablet, it includes 1 part of garlic powder or 20 that raw material is counted in parts by weight Part or one kind in allicin composition, 25 parts or 50 parts of soda acid disintegrant, 25 parts or 45 parts of filler, 1 part of binder or 5 Part, draws 1 part or 3 parts of humectant at 1 part or 5 parts of lubricant, wherein it includes alliin 0.1 to count allicin composition in parts by weight Part or 5 parts, 0.1 part or 5 parts of allinase, soda acid disintegrant includes acid source and alkali source.
Embodiment 3：As above-described embodiment 1 to the optimization of embodiment 2, acid source is citric acid, boric acid, tartaric acid, apple One or more of acid, alkali source are one or more of sodium bicarbonate, saleratus, sodium carbonate；Or/and filler is crystallite One or more of cellulose, dextrin, lactose, mannitol, sucrose；Or/and binder is hydroxypropyl methylcellulose aqueous solution, gathers Vinylpyrrolidone ethanol solution, olefin(e) acid resin aqueous solution, starch slurry, syrup, concentration of volume percent are molten for 95% ethyl alcohol One or more of liquid；Or/and lubricant is sodium benzoate, enuatrol, sodium chloride, sodium acetate, sldium lauryl sulfate, L- bright One or more of one kind, magnesium stearate in propylhomoserin, Macrogol 4000 and Macrogol 6000；Or/and it is chlorine to draw humectant Change sodium.
Embodiment 4：As above-described embodiment 1 to the optimization of embodiment 3, acid source is citric acid or tartaric acid, and alkali source is carbon The mass ratio of sour hydrogen sodium, acid source and alkali source is 5 to 7：5 to 7；Or/and binder is polyvinylpyrrolidone ethanol solution；Or/ It is one or more of Macrogol 6000 and magnesium stearate with, lubricant.
Acid source is incremented to 7 by 5, and alkali source is decremented to 5 by 7.
Embodiment 5：The anti-fungal infection allicin effervescent tablet, full pelletizing press sheet obtain as steps described below：The first step, The desired amount of allicin composition or garlic powder are weighed, is uniformly mixed, obtains with the desired amount of acid source, filler, binder Softwood material；Softwood material sieving mesh screen point is obtained wet grain material by second step；Third walks, by wet grain material at 20 DEG C to 40 Under the conditions of DEG C, after dry 2h to 5h, sieving mesh screen point obtains dry granular material；4th step, by dry granular material and the desired amount of alkali Source, lubricant, draw humectant be uniformly mixed, tabletting obtains anti-fungal infection allicin effervescent tablet；
Half granulation tabletting obtains as steps described below：The first step weighs the desired amount of allicin composition or garlic powder, It is uniformly mixed with the desired amount of acid source, filler, binder, obtains softwood material；Second step, by softwood material sieving mesh screen Point, obtain wet grain material；Third walks, by wet grain material under the conditions of 20 DEG C to 40 DEG C, after dry 2h to 5h, and sieving mesh screen point, Obtain dry granular material；4th step by dry granular material and the desired amount of alkali source, lubricant, is drawn humectant and is uniformly mixed, and tabletting obtains Anti-fungal infection allicin effervescent tablet；
Pelletizing press sheet is separated to obtain as steps described below：The first step weighs the desired amount of allicin composition or garlic Powder is uniformly mixed with the desired amount of acid source, filler, binder, obtains softwood material one, and softwood material is sieved by second step Mesh screen point, obtains wet grain material one, and third step after dry 2h to 5h, crosses sieve by wet grain material under the conditions of 20 DEG C to 40 DEG C Screening, obtains dry granular material one, the 4th step, then weigh the desired amount of allicin composition or garlic powder, with the desired amount of alkali Source, filler, binder are uniformly mixed, and obtain softwood material two, by the sieving mesh screen point of softwood material two, obtain wet grain material Two, by wet grain material two under the conditions of 20 DEG C to 40 DEG C, after dry 2h to 5h, sieving mesh screen point obtains dry granular material two, and the 5th Step, by dry granular material one, dry granular material two, with the desired amount of lubricant, draw humectant and be uniformly mixed, tabletting obtains antimycotic sense Contaminate allicin effervescent tablet.
Embodiment 6：The anti-fungal infection allicin effervescent tablet, full pelletizing press sheet obtain as steps described below：The first step, The desired amount of allicin composition or garlic powder are weighed, is uniformly mixed, obtains with the desired amount of acid source, filler, binder Softwood material；Softwood material sieving mesh screen point is obtained wet grain material by second step；Third walks, by wet grain material at 20 DEG C or 40 Under the conditions of DEG C, after dry 2h or 5h, sieving mesh screen point obtains dry granular material；4th step, by dry granular material and the desired amount of alkali Source, lubricant, draw humectant be uniformly mixed, tabletting obtains anti-fungal infection allicin effervescent tablet；
Half granulation tabletting obtains as steps described below：The first step weighs the desired amount of allicin composition or garlic powder, It is uniformly mixed with the desired amount of acid source, filler, binder, obtains softwood material；Second step, by softwood material sieving mesh screen Point, obtain wet grain material；Third walks, by wet grain material under the conditions of 20 DEG C or 40 DEG C, after dry 2h or 5h, and sieving mesh screen point, Obtain dry granular material；4th step by dry granular material and the desired amount of alkali source, lubricant, is drawn humectant and is uniformly mixed, and tabletting obtains Anti-fungal infection allicin effervescent tablet；
Pelletizing press sheet is separated to obtain as steps described below：The first step weighs the desired amount of allicin composition or garlic Powder is uniformly mixed with the desired amount of acid source, filler, binder, obtains softwood material one, and softwood material is sieved by second step Mesh screen point, obtains wet grain material one, third step, by wet grain material 20 DEG C to or under the conditions of 40 DEG C, after dry 2h or 5h, sieving Mesh screen point, obtains dry granular material one, the 4th step, then weigh the desired amount of allicin composition or garlic powder, and the desired amount of Alkali source, filler, binder are uniformly mixed, and obtain softwood material two, by the sieving mesh screen point of softwood material two, obtain wet grain material Two, by wet grain material two under the conditions of 20 DEG C or 40 DEG C, after dry 2h or 5h, sieving mesh screen point obtains dry granular material two, and the 5th Step, by dry granular material one, dry granular material two, with the desired amount of lubricant, draw humectant and be uniformly mixed, tabletting obtains antimycotic sense Contaminate allicin effervescent tablet.
Embodiment 7：As above-described embodiment 1 to the optimization of embodiment 6, garlic powder contains alliin and allinase, wherein garlic Propylhomoserin weight content is 3% to 99%；Or/and alliin purity is more than or equal to 50%, allinase vigor is more than or equal to 1000U/g.
Embodiment 8：As above-described embodiment 5 to the optimization of embodiment 7, mesh screen timesharing of being sieved, sieve is 20 mesh to 40 mesh.
Embodiment 9：Application of the anti-fungal infection allicin effervescent tablet in anti-fungal infection and sterilization.
Embodiment 10：The anti-fungal infection allicin effervescent tablet, it includes 10 parts of garlic powder, Chinese holly that raw material is counted in parts by weight 20 parts of rafter acid, 26 parts of sodium bicarbonate, 26.5 parts of lactose, 10 parts of microcrystalline cellulose, 2 parts of PVP ethanol solutions, Macrogol 6000 3 Anti-fungal infection garlic is made according to the preparation method of 6 full pelletizing press sheet of embodiment in part, 2 parts of sodium chloride, 0.5 part of magnesium stearate Capsaicin effervescent tablet.
Embodiment 11：The anti-fungal infection allicin effervescent tablet, it includes 20 parts of garlic powder, Chinese holly that raw material is counted in parts by weight It is 20 parts of rafter acid, 26 parts of sodium bicarbonate, 26.5 parts of lactose, 2 parts of PVP ethanol solutions, 3 parts of Macrogol 6000,2 parts of sodium chloride, hard Anti-fungal infection allicin effervescent tablet is made according to the preparation method of 6 half granulation tabletting of embodiment in 0.5 part of fatty acid magnesium.
Embodiment 12：The anti-fungal infection allicin effervescent tablet, it includes 1 part of alliin, garlic that raw material is counted in parts by weight 1 part of enzyme, 20 parts of tartaric acid, 27 parts of sodium bicarbonate, 44 parts of lactose, 2 parts of PVP ethanol solutions, 2 parts of Macrogol 6000, sodium chloride Anti-fungal infection allicin effervesce is made in 2 parts, 1 part of magnesium stearate, the preparation method that pelletizing press sheet is separated according to embodiment 6 Piece.
In the anti-fungal infection allicin effervescent tablet body obtained according to above-described embodiment 10, embodiment 11, embodiment 12, External outer pharmacodynamic experiment.
Laboratory sample
Anti-fungal infection allicin effervescent tablet：Experimental products prepared by the present invention
Positive control drug：Garlicinenteric-coated tablets (commercially available), lot number 30 140103, Xinjiang Tianshan pharmaceuticals industry Products
1 anti-fungal infection allicin effervescent tablet Pharmacodynamics in vitro is tested：Minimum inhibitory concentration (MIC) and minimum bactericidal are dense Spend the measurement of (MFC)
1.1 experiment material
1.1.1 experimental strain
Mycotoruloides, aspergillus, Penicillium, Piedraia, trichophyton, Microsporon, epidermis tinea are chosen in experiment Pseudomonas etc. 9 belongs to 17 kinds of 221 plants of common causative fungies.
1.1.2 culture medium
To contain 2% glucose sugar RPMI-1640 fluid nutrient mediums as basic culture medium without any antibiotic.
1.2 experimental method
Referring in particular to U.S. clinical and Laboratory Standard research institute (Clinical and laboratory Standards institute, US, CLSI) promulgate saccharomycete M-27A3 and filamentous fungi M-38P2 standard method carry out Experiment.
1.2.1 prepared by sample solution
Anti-fungal infection allicin effervescent tablet after weighing is put into a certain amount of water, and slice, thin piece is disintegrated rapidly, disintegration Time is less than 5 minutes.
It is pulverized with mortar after positive control drug garlicinenteric-coated tablets are weighed, is added in quantitative 0.1M PBS (PH 7.2) and fills Divide dissolving.Note：Complete enteric coatel tablets need to be disintegrated completely for 60 minutes in 0.1M PBS releases active ingredient, molten to accelerate It solves this experiment and first pulverizes enteric coatel tablets and add 0.1M PBS solutions (simulation enteric liquid).
The concentration of allicin in two samples is measured before experiment, and is adjusted to 1mg/ml as working stock, first uses PBS 32 times of dilution, the as working solution of 32 μ g/ml make 10 concentration of doubling dilution successively, make the drug concentration be：16μg/ml、8μg/ ml、4μg/ml、2μg/ml、1μg/ml、0.5μg/ml、0.25μg/ml、0.125μg/ml、0.06μg/ml、0.03μg/ml。
1.2.2 the measurement of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MFC)
Above-mentioned experimental bacteria is first activated, on the weak formula culture medium (SDA) in husky fort, 30 DEG C are cultivated 48 hours saccharomycete；It is Filamentous On potato dextrose agar (PDA) culture medium, 26 DEG C are cultivated 7 days to 10 days fungi；It is outstanding it to be made into bacterium with sterile saline Liquid, with spectrophotometer than turbid, wavelength 600nm；The bacteria suspension that each bacterium prepared is adjusted by this, with RPMI-1640 culture mediums 50 times are diluted again, takes 0.1ml bacterium solutions, are added in the culture plate of fresh drug coated.After liquid and bacterium solution mixing, 30 DEG C of trainings are placed It supports and is incubated in case.
Quantity of microorganism inoculated：Saccharomycete：0.5×103Cfu/ml to 2.5 × 103cfu/ml；Filamentous fungi：0.4cfu/ml to 5 × 104cfu/ml
Condition of culture：Saccharomycete：35 DEG C 48 hours to 72 hours
Filamentous fungi：30 DEG C 7 days
1.2.3 result judgement method：
(1) minimum inhibitory concentration (MIC)：It is grown to reference with blank control and Vehicle controls pore fungi, bacterium in medicine datum hole Non- growth table is shown with bacteriostasis, and bacterium, which has grown, indicates no bacteriostasis；The lowest concentration of drug that bacterium does not grow is MIC, uses μ G/ml is indicated.
(2) minimum bactericidal concentration (MFC)：By the repressed bacterium solution of medicine datum hole, after being blown and beaten repeatedly with suction nozzle, 0.1ml is taken out It adds in not antibiotic sand fort liquid-based 2ml, mixing is cultivated 7 days at 26 DEG C.If there is bacterium is grown to only bacteriostasis, Without bactericidal effect；If growth table is not shown with bactericidal effect yet, the lowest concentration of drug that bacterium does not grow is MFC, with μ g/ml tables Show.
Experiment sets 3 parallel holes by regulation every time, is made 2 times of meter before and after duplicate methods experiment.To ensure experiment Accuracy and authenticity.
1.3 result
1.3.1 17 kinds 221 plants of anti-fungal infection allicin effervescent tablet and the category of positive control sample garlicinenteric-coated tablets pair 9 are common Pathogenic bacteria minimum inhibitory concentration (MIC) measurement result is summarized in table 1.Table 1 is that anti-fungal infection allicin effervescent tablet resists 9 in vitro Belong to 17 kinds of 221 plants of common pathogen MIC measurement results, wherein A is anti-fungal infection allicin effervescent tablet；B is positive control Medicine allicin enteric coatel tablets.
1.3.2 allicin effervescent tablet and garlicinenteric-coated tablets pair 9 belong to 17 kinds of 221 plants of common pathogen minimum bactericidal concentrations (MFC) measurement result is shown in Table 2.Table 2 is that anti-fungal infection allicin effervescent tablet belongs to 17 kinds of 221 plants of common pathogens to 9 in vitro The measurement result of MFC, wherein A is anti-fungal infection allicin effervescent tablet；B is positive control drug allicin enteric coatel tablets.
It can be seen that the active ingredient garlic of anti-fungal infection allicin effervescent tablet of the present invention according to table 1,2 data of table Capsaicin belongs to 17 kinds of 221 plants of bacterium MIC measurement results to common causative fungi 9：MIC (221) range 0.25 μ g/ml to 4.0 μ g/ml, MIC50=1.0 μ g/ml, MIC90It is the features such as=2.0 μ g/ml, antimicrobial spectrum is relatively wide, antibacterial action is stronger, big with positive control drug The result of allicin enteric coatel tablets is more consistent.Measurement result MFC (221) range (1.0 μ g/ml to 16 μ g/ml) of MFC, MFC50= 4.0 μ g/ml, MFC90=8.0 μ g/ml, MIC and MFC differ about 4 times, it was demonstrated that anti-fungal infection allicin effervescent tablet of the present invention Existing stronger bacteriostasis also has stronger bactericidal effect simultaneously.
2 anti-fungal infection allicin effervescent tablet antifungal ATCC5314 and clinical strains mouse system infection model Protective effect experiment
2.1 material
2.1.1 experimental animal
ICR mouse (cleaning grade) Yangzhou University's comparative medicine center provides (animal quality certification number SCXK (Soviet Union 2012- 0004)), 6 week old, weight 18.0g to 20.0g are random to be grouped；Every group 10, half male and half female, while setting up experimental group, infection Control group and blank control group.
1.1.2 infection bacteria species：China Microbiological bacterium (poison) preservation administration committee, the white beads of medical mycology center preservation Bacterium ATCC5314.Clinical strains：It detaches from blood samples of patients, is presented by Shanghai Huashan Hospital of Fudan University professor Zhang Qiangqiang (August detaches on the 22nd within 2013, and original culture number is 133750)；After identified confirmation, Ministry of Public Health medical microbial bacterium has been included (poison) plants the center strain library preservation of preservation administrative center fungi.Candida albicans clinical strain, strain CMCC (F) C.1V(referred to as C.1V)；First restore virulence in Mice Body before formal experiment, it is spare.
2.2 method
2.2.1 route of infection：Sexual assault of mouse tail vein.
2.2.2 the determination of infection dosage：By the above-mentioned bacterial strain candida albicans ATCC5314 activated and clinical strain CMCC (F) C.1VIt is incubated 48 hours for 30 DEG C after the weak agar medium passage in husky fort, fresh mature bacterium colony is washed down with physiology salt respectively, is passed through After blood counting chamber counts, adjustment concentration to 2.0 × 107Cfu/ml is diluted to the bacteria suspension of 5 gradients, concentration difference successively again It is 2.0 × 107cfu/ml、1.0×107cfu/ml、2.0×106cfu/ml、1.0×106cfu/ml、2.0×105Cfu/ml is standby With.The injection volume of bacterium amount when being attacked as this subinfection tail vein, every mouse is 0.5ml/, measures animal dead day Number, determines in 10 days the dead minimum infection dosage (100%MLD) of mouse 100%, infection dosage when allicin Protection For 1MLD to 2MLD.
2.2.3 experimental drug anti-fungal infection allicin effervescent tablet and positive control drug (garlicinenteric-coated tablets) are to candida albicans The Protection of system infections.
1) preliminary experiment dosage：
A anti-fungal infection allicin effervescent tablets
①30mg/kg②15mg/kg③5mg/kg④1.0mg/kg⑤0.5mg/kg
B positive control drugs-garlicinenteric-coated tablets
①30mg/kg②15mg/kg③5mg/kg④1.0mg/kg⑤0.5mg/kg
2) formal experimental administration dosage：
A anti-fungal infection allicin effervescent tablets
①10mg/kg②7.0mg/kg③4.9mg/kg④3.4mg/kg⑤2.4mg/kg
B positive control drugs-garlicinenteric-coated tablets
①10mg/kg②7.0mg/kg③4.9mg/kg④3.4mg/kg⑤2.4mg/kg
It is to further look at experimental drug anti-fungal infection allicin effervescent tablet and positive control drug garlic in formal experiment For enteric coatel tablets after disposable drug when 96h, each group mouse kidney carries bacterium amount, the method counted using bacterium colony, acquired results Carry out statistical analysis.Therefore every group of experimental animal increases to 14 (7 ♂, 7 ♀), 96h takes 4 (2 ♂, 2 ♀) to be used for bacterium counting experiments, Remaining 10 are still used to observe protective effect of drug.
3) administration route and method：
It is administered in 2 hours to 4 hours after mouse tail vein bacterium is attacked, dosage presses every average mice body weight about 20.0g is calculated, and is dissolved in 0.5mlPBS, disposable gavage；Infection control group and blank control group give PBS as placebo.
2.2.4 observation method：
After administration for 24 hours in dead mouse, observation data must not be included in.When infection control is all dead, each group is counted Surviving animals number, then data statistics is carried out, statistical result data are as shown in table 3, table 4.Table 3 is candida albicans (ATCC SC5314) the average number of days of death of system infections difference bacterium amount ICR mouse, table 4 are candida albicans CMCC (F) C.1VSystem infections The average number of days of death of different bacterium amount ICR mouse.
The determination of 2.3 infection dosages
1.0 × 10 can be determined according to table 3,4 data of table6Cfu/ is only using this bacterium amount as whole death in 10 days 100%MLD, the formal infection dosage for testing candida albicans system infections model are controlled in 1MLD between 2MLD.
2.3.1 preliminary experiment
Anti-fungal infection allicin effervescent tablet and positive control drug garlicinenteric-coated tablets of the present invention are oral to the white beads of mouse Bacterium ATCC5314 and clinical strains candida albicans CMCC (F) C.1VThe preliminary experiment of the Protection of system infections model, data such as table 5, shown in table 6.Table 5 is the protective effect to candida albicans ATCC5314 system infections mouse, and table 6 is to candida albicans CMCC (F)C.1VThe protective effect of system infections mouse.
Can be seen that quantity of microorganism inoculated according to 5 data of table is 1.5 × 106Cfu/ only, infects the practical survival number of days of control group 8.55 ± 1.619 days, blank control group mouse health was without exception during experiment, all survives.P>0.05.
Can be seen that quantity of microorganism inoculated according to 6 data of table is 1.5 × 106Cfu/ only, infects the practical survival number of days of control group 6.65 ± 1.991 days, blank control group mouse health was without exception during experiment, all survives.P>0.05.
2.3.2 formal experiment
It is oral to further look at experimental drug anti-fungal infection allicin effervescent tablet and positive control drug garlicinenteric-coated tablets To mouse candida albicans ATCC5314 and clinical strains candida albicans CMCC (F) C.1VThe Protection of system infections model is just Formula is tested, after disposable experimental drug anti-fungal infection allicin effervescent tablet and positive control drug garlicinenteric-coated tablets when 96h, Each group mouse kidney carries bacterium amount, and the method counted using bacterium colony, acquired results carry out statistical analysis.Therefore every group of experimental animal increases It adding to 14 (7 ♂, 7 ♀), 96h takes 4 (2 ♂, 2 ♀) for bacterium counting experiments, remaining 10 are still used to observe protective effect of drug, The data obtained is as shown in table 7, table 8.Table 7 is that 96 hours kidney candida albicans ATCC5314 carry bacterium amount feelings to each experimental group upon administration Condition, table 8 are each experimental group 96 hours kidney candida albicans CMCC (F) C. upon administration1VCarry bacterium amount situation.
2.4 result
ATCC5314 plants of candida albicans is can be seen that according to table 5, table 6, table 7,8 data of table：Anti-fungal infection allicin ED50=5.1142 (4.1125 to 6.3597) mg/kg of effervescent tablet, positive control drug garlicinenteric-coated tablets ED50=5.3999 (4.097 to 7.1171) mg/kg；Candida albicans clinical strain CMCC (F) C.1V：The ED50 of anti-fungal infection allicin effervescent tablet =4.5416 (3.7512 to 5.4987) mg/kg, positive control drug garlicinenteric-coated tablets ED50=5.3243 (4.2316 to 6.6991)mg/kg.96 hours each group Mouse Kidneys carry bacterium amount count results and show between different bacterial strains slightly difference after administration, But the inhibiting rate of bacterium is also correspondingly improved with the increase of drug dose between each dosage group, anti-fungal infection allicin bubble It is essentially identical to the protective effect size of candida albicans system infections mouse model with positive control drug garlicinenteric-coated tablets to rise piece.
Therefore, anti-fungal infection allicin effervescent tablet of the present invention has disintegration time limited short (being less than 5 minutes), works rapidly The characteristics of, and garlicinenteric-coated tablets need to be disintegrated completely for 60 minutes in intestinal juice and release active ingredient, while anti-fungal infection Allicin effervescent tablet can in use, also can external application, such as outside be used for vagina, increase active ingredient allicin and vagina fold The contact area at position, local concentration are high, are more advantageous to its and play therapeutic effect, and garlicinenteric-coated tablets only oral administration.
In conclusion the present invention is using alliin and allinase or garlic powder as raw material, it is former with acid source, alkali source and other auxiliary Material is mixed and made into allicin effervescent tablet, and raw material is pure natural garlic extract, is had no toxic side effect, to pathomycete and its resistance to Medicine bacterium have it is strong kill inhibiting effect, both can external application, also oral administration, used time be also easy to store and transport.The antimycotic sense of the present invention Dye allicin effervescent tablet has the characteristics that quick, efficient, stable anti-fungal infection, is applied to woman especially as vagina effervescence The therapeutic effect of section's infectious disease is more preferably.
The above technical characteristic constitutes the embodiment of the present invention, can basis with stronger adaptability and implementation result Actual needs increases and decreases non-essential technical characteristic, to meet the needs of different situations.
Table 1
Table 2
Table 3
Bacterium amount Experimental animal number Survival number of days Average Survival number of days (X ± SD)
1.0×107 10 4.0 to 8.0 6.5 ± 1.43 (n=10)
0.5×107 10 4.0 to 11.0 7.2 ± 2.29 (n=10)
1.0×106 10 5.0 to 13.0 9.1 ± 2.77 (n=10)
0.5×105 10 6.0 to 15.0 10.2 ± 2.53 (n=10)
1.0×105 10 6.0 to 17.0 11.8 ± 3.49 (n=10)
Table 4
Bacterium amount Experimental animal number Survival number of days Average Survival number of days (X ± SD)
1.0×107 10 2.5 to 9.50 5.4 ± 2.256 (n=10)
0.5×107 10 4.5 to 10.0 7.2 ± 2.216 (n=10)
1.0×106 10 5.5 to 12.0 9.7 ± 2.027 (n=10)
0.5×106 10 7.5 to 26.5 14.4 ± 5.356 (n=10)
1.0×105 10 9.5 to 17.5★ 12.83 ± 3.078 (n=6)
Table 5
Table 6
Table 7
Table 8

Claims (10)

1. a kind of anti-fungal infection allicin effervescent tablet, it is characterised in that raw material count in parts by weight include 1 part of garlic powder extremely 20 parts and one kind in allicin composition, 25 parts to 50 parts of soda acid disintegrant, 25 parts to 45 parts of filler, 1 part of binder To 5 parts, 1 part to 5 parts of lubricant, draw 1 part to 3 parts of humectant, wherein in parts by weight count allicin composition include alliin 0.1 part to 5 parts, 0.1 part to 5 parts of allinase, soda acid disintegrant includes acid source and alkali source.
2. anti-fungal infection allicin effervescent tablet according to claim 1, it is characterised in that acid source is citric acid, boron One or more of acid, tartaric acid, malic acid, alkali source are one or more of sodium bicarbonate, saleratus, sodium carbonate；Or/ It is one or more of microcrystalline cellulose, dextrin, lactose, mannitol, sucrose with, filler；Or/and binder is hydroxypropyl first Cellulose aqueous solution, polyvinylpyrrolidone ethanol solution, olefin(e) acid resin aqueous solution, starch slurry, syrup, concentration of volume percent For 95% one or more of ethanol solution；Or/and lubricant is sodium benzoate, enuatrol, sodium chloride, sodium acetate, bay One or more of one kind, magnesium stearate in alcohol sodium sulphate, L-Leu, Macrogol 4000 and Macrogol 6000；Or/ With, draw humectant be sodium chloride.
3. anti-fungal infection allicin effervescent tablet according to claim 2, it is characterised in that acid source is citric acid or wine Stone acid, alkali source is sodium bicarbonate, and the mass ratio of acid source and alkali source is 5 to 7：5 to 7；Or/and binder is polyvinylpyrrolidine Ketone ethanol solution；Or/and lubricant is one or more of Macrogol 6000 and magnesium stearate.
4. anti-fungal infection allicin effervescent tablet according to claim 1 or 2 or 3, it is characterised in that using full granulation Tabletting method is prepared, and full pelletizing press sheet method carries out as steps described below：The first step weighs the desired amount of allicin group Object or garlic powder are closed, is uniformly mixed with the desired amount of acid source, filler, binder, obtains softwood material, second step, by softwood Material sieving mesh screen point, obtains wet grain material, and third walks, by wet grain material under the conditions of 20 DEG C to 40 DEG C, after dry 2h to 5h, Be sieved mesh screen point, obtains dry granular material, the 4th step, by dry granular material and the desired amount of alkali source, lubricant, draws humectant and mixes Even, tabletting obtains anti-fungal infection allicin effervescent tablet；Alternatively, being prepared using half granulation tabletting method, half granulation pressure Piece carries out as steps described below：The first step weighs the desired amount of allicin composition or garlic powder, with the desired amount of acid source, Filler, binder are uniformly mixed, and obtain softwood material, and softwood material sieving mesh screen point is obtained wet grain material by second step, Third walks, and by wet grain material under the conditions of 20 DEG C to 40 DEG C, after dry 2h to 5h, sieving mesh screen point obtains dry granular material, and the 4th Step by dry granular material and the desired amount of alkali source, lubricant, is drawn humectant and is uniformly mixed, and tabletting obtains anti-fungal infection allicin Effervescent tablet；Alternatively, being prepared using pelletizing press sheet method is separated, separates pelletizing press sheet and carry out as steps described below：The first step, The desired amount of allicin composition or garlic powder are weighed, is uniformly mixed, obtains with the desired amount of acid source, filler, binder The sieving mesh screen point of softwood material one is obtained wet grain material one by softwood material one, second step, and third step exists wet grain material one Under the conditions of 20 DEG C to 40 DEG C, after dry 2h to 5h, sieving mesh screen point obtains dry granular material one, the 4th step, then weigh the desired amount of Allicin composition or garlic powder are uniformly mixed with the desired amount of alkali source, filler, binder, obtain softwood material two, will The sieving mesh screen point of softwood material two, obtains wet grain material two, by wet grain material two under the conditions of 20 DEG C to 40 DEG C, dry 2h to 5h Afterwards, sieving mesh screen point, obtains dry granular material two, the 5th step, by dry granular material one, dry granular material two, with the desired amount of lubricant, Draw humectant to be uniformly mixed, tabletting obtains anti-fungal infection allicin effervescent tablet.
5. anti-fungal infection allicin effervescent tablet according to claim 1 or 2 or 3 or 4, it is characterised in that garlic powder contains There are alliin and allinase, wherein alliin weight content is 3% to 99%；Or/and alliin purity is more than or equal to 50%, garlic Enzyme activity is more than or equal to 1000U/g.
6. anti-fungal infection allicin effervescent tablet according to claim 4 or 5, it is characterised in that sieving mesh screen timesharing, Sieve is 20 mesh to 40 mesh.
7. a kind of preparation method of anti-fungal infection allicin effervescent tablet according to claim 1 or 2 or 3 or 4, special Sign is to carry out using full pelletizing press sheet method, and full pelletizing press sheet method carries out as steps described below：The first step weighs aequum Allicin composition or garlic powder, be uniformly mixed with the desired amount of acid source, filler, binder, obtain softwood material, Softwood material sieving mesh screen point is obtained wet grain material by two steps, and third step is done by wet grain material under the conditions of 20 DEG C to 40 DEG C After dry 2h to 5h, sieving mesh screen point obtains dry granular material, the 4th step, by dry granular material and the desired amount of alkali source, lubricant, draws Humectant is uniformly mixed, and tabletting obtains anti-fungal infection allicin effervescent tablet；Alternatively, being carried out using half granulation tabletting method, half Pelletizing press sheet carries out as steps described below：The first step weighs the desired amount of allicin composition or garlic powder, and the desired amount of Acid source, filler, binder are uniformly mixed, and obtain softwood material, and softwood material sieving mesh screen point is obtained wet grain by second step Material, third step, by wet grain material under the conditions of 20 DEG C to 40 DEG C, after dry 2h to 5h, sieving mesh screen point obtains dry granular object Material, the 4th step by dry granular material and the desired amount of alkali source, lubricant, are drawn humectant and are uniformly mixed, and tabletting obtains anti-fungal infection Allicin effervescent tablet；Alternatively, being carried out using pelletizing press sheet method is separated, separates pelletizing press sheet and carry out as steps described below：The One step weighs the desired amount of allicin composition or garlic powder, is uniformly mixed with the desired amount of acid source, filler, binder, Softwood material one is obtained, the sieving mesh screen point of softwood material one is obtained wet grain material one, third step, by wet grain material by second step One under the conditions of 20 DEG C to 40 DEG C, and after dry 2h to 5h, sieving mesh screen point obtains dry granular material one, the 4th step, then weighs required The allicin composition or garlic powder of amount, are uniformly mixed with the desired amount of alkali source, filler, binder, obtain softwood material Two, by the sieving mesh screen point of softwood material two, wet grain material two is obtained, it is dry by wet grain material two under the conditions of 20 DEG C to 40 DEG C After 2h to 5h, sieving mesh screen point obtains dry granular material two, the 5th step, by dry granular material one, dry granular material two, with it is the desired amount of Lubricant, draw humectant be uniformly mixed, tabletting obtains anti-fungal infection allicin effervescent tablet.
8. the preparation method of anti-fungal infection allicin effervescent tablet according to claim 7, it is characterised in that garlic powder Contain alliin and allinase, wherein alliin weight content is 3% to 99%；Or/and alliin purity is more than or equal to 50%, Allinase vigor is more than or equal to 1000U/g.
9. the preparation method of anti-fungal infection allicin effervescent tablet according to claim 7 or 8, it is characterised in that sieving Mesh screen timesharing, sieve are 20 mesh to 40 mesh.
10. a kind of anti-fungal infection allicin effervescent tablet according to claims 1 or 2 or 3 or 4 or 5 or 6 is anti-in preparation Application in fungal infection or/and sterilization effervescent tablet.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114806993A (en) * 2022-05-27 2022-07-29 山东大学 Bacterium enzyme composition for preventing and treating root-knot nematode and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1201686A (en) * 1998-06-16 1998-12-16 胡小波 Garlic powder capsule
CN101549151A (en) * 2009-02-13 2009-10-07 新疆埃乐欣药业有限公司 Alliin/alliinase dualistic drug-releasing multilayer tablet
CN107094811A (en) * 2017-05-31 2017-08-29 云南省盐业有限公司 A kind of saliferous sterilization effervescent tablet used for aquiculture and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1201686A (en) * 1998-06-16 1998-12-16 胡小波 Garlic powder capsule
CN101549151A (en) * 2009-02-13 2009-10-07 新疆埃乐欣药业有限公司 Alliin/alliinase dualistic drug-releasing multilayer tablet
CN107094811A (en) * 2017-05-31 2017-08-29 云南省盐业有限公司 A kind of saliferous sterilization effervescent tablet used for aquiculture and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114806993A (en) * 2022-05-27 2022-07-29 山东大学 Bacterium enzyme composition for preventing and treating root-knot nematode and preparation method thereof
CN114806993B (en) * 2022-05-27 2023-11-21 山东大学 Bacterial enzyme composition for preventing and controlling root-knot nematode and preparation method thereof

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Application publication date: 20180824