CN108432816A - The extracting method and active constituent of common rabdosia leaf active constituent are in application agriculturally - Google Patents
The extracting method and active constituent of common rabdosia leaf active constituent are in application agriculturally Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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Abstract
The present invention provides a kind of extracting methods of common rabdosia leaf active constituent and its extract to prepare the application in inhibiting agriculture disease fungus active medicine, belongs to botanical pesticide technology field.Extracting method provided by the invention includes after crushing common rabdosia leaf, it is mixed with organic solvent, carry out the microwave of four-stage, obtain extract, and the extract has agriculture disease fungus such as Pear black spot bacterium, black rot of pear bacterium, pears anthrax bacteria, Pyricularia oryzae, mould and aspergillus niger etc. certain restraining and sterilizing bacteria ability, can be applied to the protective agents for preparing corresponding germ.Extracting method of the present invention is simple and quick, and raw material sources are extensive, does not have specific demand to instrument, is suitable for large-scale production.
Description
Technical field
The invention belongs to botanical pesticide fields, and in particular to the extracting method and extract of a kind of common rabdosia leaf extract exist
Application in agricultural.
Background technology
Disease be influence agricultural production one of principal element, cause every year the underproduction loss reach total output 10%~
15%, 70% or more is caused by disease fungus among these.For a long time, chemical pesticide be prevent fungal disease it is main very
To unique approach.The chronic administration of chemical pesticide causes serious security risks to human health and ecological environment.It finds
Safely and efficiently fungal disease control method is problem extremely urgent in current agricultural production.Botanical pesticide is thin with plant
The active material or plant tissue that born of the same parents generate are as pesticide, with raw material sources are extensive, toxic side effect is small, pacify to non-target organism
The advantages that complete and environmentally friendly, has become the new trend of global pesticide research and development at present.
Common rabdosia leaf (Isodon amethystoides (Benth) Cy Wu et Hsuan) is planted for Labiatae Rabdosia
Object has antibacterial anti-inflammatory, anti-oxidant, antitumor, the functions such as antithrombotic civil containing there are many effective medicinal ingredients in common rabdosia leaf
" be sore be not sore, three bowls of common rabdosia leaf soup " is spread, so the research to common rabdosia leaf focuses mostly in the treatment of disease, such as in China
Just it is applied to preparation treatment autoimmune disease drug and Chinese patent in patent CN03112870.X
In CN1541643A treatment autoimmune is used to prepare with Cellular immunity suppression and humoral immunity inhibiting effect using it
Disease medicament, but there is no any about the record being applied to common rabdosia leaf in rural activity.
Invention content
In view of this, the purpose of the present invention is to provide a kind of extracting method of common rabdosia leaf active constituent, extract
Active constituent has good control effect to the fungal disease in agricultural production, and solve that currently used chemical pesticide brings is strong
The problem of health, environment and ecology aspect.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
The present invention provides a kind of extracting methods of common rabdosia leaf active constituent, include the following steps：
1) after crushing common rabdosia leaf, according to 1kg：The mass volume ratio of (1~5) L is mixed with organic solvent, and it is micro- to carry out first
Wave extracts；The power of first microwave is 50~200W, and the time of first microwave is 120~180s；
2) the second Microwave Extraction is carried out after step 1) first microwave, the power of second microwave is 45~50W, institute
The time for stating the second microwave is 350~450s；
3) third Microwave Extraction is carried out after step 2) second microwave, the power of the third microwave is 50~100W,
The time of the third microwave is 180~220s；
4) the 4th Microwave Extraction is carried out after step 3) the third microwave, the power of the 4th microwave is 40~50W, institute
The time for stating the 4th microwave is 550~650s, obtains common rabdosia leaf active constituent.
Preferably, the grain size of the step 1) crushing is 170~230 mesh.
Preferably, the step 1) organic solvent includes saturated monohydroxy alcohol class, saturated ketone, esters or chloroform.
Preferably, the temperature of step 1) first microwave is 40~70 DEG C.
Preferably, the temperature of step 2) second microwave is 35~65 DEG C.
Preferably, the temperature of step 3) the third microwave is 35~65 DEG C.
Preferably, further include rotating the extract after step 4) the 4th microwave, vacuum drying.
The present invention also provides a kind of common rabdosia leaf active constituents extracted using said extracted method.
The present invention also provides a kind of application of above-mentioned common rabdosia leaf active constituent in inhibiting agriculture disease fungus activity.
Preferably, the agriculture disease fungus include Pear black spot bacterium, black rot of pear bacterium, pears anthrax bacteria, Pyricularia oryzae,
Mould and aspergillus niger.
The present invention provides a kind of extracting methods of common rabdosia leaf active constituent and its extract to prepare the agriculture cause of disease of inhibition
Application in fungi activity drug.The active constituent being separated to using multistage microwave―assisted extraction includes mainly common rabdosia leaf first
Element, common rabdosia leaf B prime and common rabdosia leaf C prime, the content in common rabdosia leaf blade reach the 2.23% of dry weight, 1.63% and
0.21%.These active constituents can effectively inhibit the proliferation of disease fungus.
Specific implementation mode
The present invention provides a kind of extracting methods of common rabdosia leaf active constituent, include the following steps：
1) after crushing common rabdosia leaf, according to 1kg：The mass volume ratio of (1~5) L is mixed with organic solvent, and it is micro- to carry out first
Wave extracts；The power of first microwave is 50~200W, and the time of first microwave is 120~180s；
2) the second Microwave Extraction is carried out after step 1) first microwave, the power of second microwave is 45~50W, institute
The time for stating the second microwave is 350~450s；
3) third Microwave Extraction is carried out after step 2) second microwave, the power of the third microwave is 50~100W,
The time of the third microwave is 180~220s；
4) the 4th Microwave Extraction is carried out after step 3) the third microwave, the power of the 4th microwave is 40~50W, institute
The time for stating the 4th microwave is 550~650s, obtains common rabdosia leaf active constituent.
The present invention is when extracting common rabdosia leaf active constituent, after common rabdosia leaf is crushed, according to 1kg：The quality volume of (1~5) L
Than being mixed with organic solvent, the first Microwave Extraction is carried out.In the present invention, the common rabdosia leaf is preferably the leaf of common rabdosia leaf, institute
Leaf is stated preferably by being dried, and the present invention does not have the method for the drying process special restriction, utilizes this field
Conventional drying processing method.The grain size of crushing of the present invention is preferably 170~230 mesh, more preferably 180~220
Mesh, most preferably 200 mesh.The present invention after being pulverized, according to 1kg：The mass volume ratio of (1~5) L is mixed with organic solvent,
The mass volume ratio is preferably 1kg：(2~4) L, more preferably 1kg:3L.
Organic solvent of the present invention includes preferably saturated monohydroxy alcohol class, saturated ketone, esters or chloroform, is had when described
When solvent is saturated monohydroxy alcohol class, the saturated monohydroxy alcohol class includes preferably ethyl alcohol, mass concentration (or the body of the ethyl alcohol
Product concentration) it is preferably 100%；When the organic solvent is saturated ketone, the saturated ketone includes preferably acetone, institute
The mass concentration (or volumetric concentration) for stating acetone is preferably 100%；When the organic solvent is esters, the esters are preferred
Include ethyl acetate, the mass concentration (or volumetric concentration) of the ethyl acetate is preferably 100%；When the organic solvent
For chloroform when, the mass concentration (or volumetric concentration) of the chloroform is preferably 100%.
The time of first microwave of the present invention is preferably 120~180s, more preferably 140~160s, most preferably
It is 150s；In the present invention, when the organic solvent difference, the preferred also not phase of the power of first microwave and temperature
Together, specifically：When the organic solvent is saturated monohydroxy alcohol, the power of first microwave is preferably 200W, and described the
The temperature of one microwave is preferably 70 DEG C；When the organic solvent is saturated ketone, the power of first microwave is preferred
Temperature for 70W, first microwave is preferably 45 DEG C；When the organic solvent is chloroform, the work(of first microwave
Rate is preferably 50W, and the temperature of first microwave is preferably 40 DEG C；When the organic solvent is esters, described first
The power of microwave is preferably 60W, and the temperature of first microwave is preferably 55 DEG C.
The present invention continues the second Microwave Extraction after completing the first microwave.The time of second microwave is preferably
350~450s, more preferably 380~420s, most preferably 400s.In the present invention, when the organic solvent difference, institute
The power for stating the second microwave is preferably also differed with temperature, specifically：It is described when the organic solvent is saturated monohydroxy alcohol
The power of second microwave is preferably 50W, and the temperature of second microwave is preferably 65 DEG C；When the organic solvent is saturation
When ketone, the power of second microwave is preferably 50W, and the temperature of second microwave is preferably 40 DEG C；When described organic
When solvent is chloroform, the power of second microwave is preferably 45W, and the temperature of second microwave is preferably 35 DEG C；When
When the organic solvent is esters, the power of second microwave is preferably 50W, and the temperature of second microwave is preferably
40℃。
The present invention carries out third Microwave Extraction after completing the second microwave.The time of the third microwave is preferably 180~
220s, more preferably 190~210s, most preferably 200s.In the present invention, when the organic solvent difference, described
The power of three microwaves is preferably also differed with temperature, specifically：When the organic solvent is saturated monohydroxy alcohol, the third
The power of microwave is preferably 100W, and the temperature of the third microwave is preferably 65 DEG C；When the organic solvent is saturated ketone
When, the power of the third microwave is preferably 60W, and the temperature of the third microwave is preferably 40 DEG C；When described organic molten
When agent is chloroform, the power of the third microwave is preferably 50W, and the temperature of the third microwave is preferably 35 DEG C；Work as institute
State organic solvent be esters when, the power of the third microwave is preferably 60W, and the temperature of the third microwave is preferably 40
℃。
The present invention carries out the 4th Microwave Extraction, obtains common rabdosia leaf active constituent after completing third microwave.4th microwave
Time be preferably 550~650s, more preferably 580~620s, most preferably 600s.In the present invention, when described organic
When solvent difference, the power of the 4th microwave preferably also differs, specifically：When the organic solvent is saturated monohydroxy alcohol
When, the power of the 4th microwave is preferably 50W；When the organic solvent is saturated ketone, the power of the 4th microwave
Preferably 50W；When the organic solvent is chloroform, the power of the 4th microwave is preferably 40W；When described organic molten
When agent is esters, the power of the 4th microwave is preferably 50W.The temperature when present invention is to four Microwave Extraction does not have
Particular determination.
The present invention further includes preferably rotating the extract after the 4th microwave, vacuum drying.The present invention couple
There is no particular determinations for the revolving and vacuum drying instrument, and the revolving is preferably in the rotatable evaporations of IKARV 10basic
It is carried out on instrument, the heating temperature of the revolving is preferably 60 DEG C, and the vacuum degree of the revolving is preferably 95~98kPa, the rotation
The rotating speed of steaming is preferably 20r/min.The vacuum drying preferably obtains dry bulk in empty DZF-6051 types vacuum drying chamber
Product.
The present invention also provides a kind of common rabdosia leaf active constituents extracted using said extracted method.In the present invention
In, the active constituent includes preferably wangzaozin A, common rabdosia leaf B prime and common rabdosia leaf C prime.
The present invention also provides a kind of application of above-mentioned common rabdosia leaf active constituent in inhibiting agriculture disease fungus activity.At this
In invention, the agricultural disease fungus includes Pear black spot bacterium, black rot of pear bacterium, pears anthrax bacteria, Pyricularia oryzae, mould and black
Aspergillus.When Extraction solvent difference in the present invention, there is inhibiting effect to different agriculture disease fungus activity, it is preferred that when
When the Extraction solvent is saturated monohydroxy alcohol, the agricultural disease fungus is black rot of pear bacterium and Pyricularia oryzae；When the extraction
When solvent is saturated ketone, the agricultural disease fungus is pears anthrax bacteria, Penicillium notatum, Pear black spot bacterium and Pyricularia oryzae；Work as institute
State Extraction solvent be chloroform when, it is described agricultural disease fungus be alternaria, black rot of pear bacterium, pears anthrax bacteria, Pyricularia oryzae,
Mould and aspergillus niger；When the Extraction solvent is esters, the agricultural disease fungus is aspergillus niger, black rot of pear bacterium, pears charcoal
Subcutaneous ulcer germ, Penicillium notatum, Pear black spot bacterium and Pyricularia oryzae.Application of the present invention preferably prepares drug, the drug
Dosage form includes preferably missible oil, suspending agent, wettable powder, pulvis, granula, aqua, poison bait, mother liquor or female powder.The present invention couple
There is no particular determinations for the preparation method of the drug, are prepared using the conventional method of this field.It is effective in drug
0.05~13.3mg/ml of content.
The extracting method of common rabdosia leaf active constituent provided by the invention is described in detail with reference to embodiment, but
It is that they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Common rabdosia leaf leaf after drying is crushed on micronizer as after 200 mesh particles, with ethyl alcohol according to 1kg:3L(m/
V solid-liquid ratio mixing), carries out four stage Microwave Extractions, obtains common rabdosia leaf active constituent, detailed process is as shown in table 1：
1. common rabdosia leaf ethanol extract of table extracts flow
Measure to obtain the common rabdosia leaf active constituent that embodiment obtains using HPLC, active component content is as shown in table 2：
2. common rabdosia leaf ethanol extract active ingredient of table and content
Black rot of pear bacterium and Pyricularia oryzae are inoculated on PDA solid mediums, dark culturing 5d at 25 DEG C of constant temperature waits for bacterium
Silk uniformly grows the PDA culture medium that the punching of rear 0.5cm card punch is paved with mycelia, be inoculated in respectively containing 13.3,10,5,2.5,1,
0.25,0.1, on the PDA solid mediums of 1 extract of 0.05mg/mL embodiments.7d is cultivated at 25 DEG C records inhibition zone, it is antibacterial
Rate is as shown in table 3：
3. common rabdosia leaf ethanol extract bacteriostasis rate of table
| 13.3 | 10 | 5 | 2.5 | 1 | 0.5 | 0.25 | 0.1 | 0.05 | |
| Pears wheel line | 100.0% | 100.0% | 66.6% | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% |
| Rice blast | 100.0% | 100.0% | 100.0% | 25.6% | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% |
It is found that common rabdosia leaf ethanol extract in 5mg/mL, has black rot of pear bacterium 66.6% bacteriostasis rate, in 10mg/
When mL, bacteriostasis rate has had reached 100%；In 5mg/mL, there is 100% bacteriostasis rate to Pyricularia oryzae.
Embodiment 2
Common rabdosia leaf leaf after drying is crushed on micronizer as after 200 mesh particles, with acetone according to 1kg:3L(m/
V solid-liquid ratio mixing), carries out four stage Microwave Extractions, detailed process is as shown in table 4：
4. common rabdosia leaf acetone extract of table extracts flow
Pears anthrax bacteria, Penicillium notatum, Pear black spot bacterium and Pyricularia oryzae are inoculated on PDA solid mediums, constant temperature 25
Dark culturing 5d at DEG C, 0.5cm card punch punches the PDA culture medium for being paved with mycelia after mycelia uniformly grows, and is inoculated in respectively
Containing 13.3,10,5,2.5,1,0.25,0.1, on the PDA solid mediums of 2 extract of 0.05mg/mL embodiments.It is cultivated at 25 DEG C
7d records inhibition zone, and bacteriostasis rate is as shown in table 5：
5. common rabdosia leaf acetone extract bacteriostasis rate of table
| 13.3 | 10 | 5 | 2.5 | 1 | 0.5 | 0.25 | 0.1 | 0.05 | |
| Pears anthrax | 100.0% | 100.0% | 100.0% | 66.7% | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% |
| Penicillium notatum | 100.0% | 100.0% | 100.0% | 100.0% | 100.0% | 100.0% | 0.0% | 0.0% | 0.0% |
| Pears blackspot | 67.3% | 40.6% | 23.5% | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% |
| Rice blast | 41.2% | 25.0% | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% |
It is found that common rabdosia leaf acetone extract in 2.5mg/mL, has pears anthrax bacteria 66.7% bacteriostasis rate, in 5mg/
When mL, bacteriostasis rate has had reached 100%；In 0.5mg/mL, just there is 100% bacteriostasis rate to Penicillium notatum；In 13.3mg/mL
When, the bacteriostasis rate to Pear black spot bacterium is 67.3%.
Embodiment 3
Common rabdosia leaf leaf after drying is crushed on micronizer as after 200 mesh particles, with chloroform according to 1kg:3L(m/
V solid-liquid ratio mixing), carries out four stage Microwave Extractions, detailed process is as shown in table 6：
6. common rabdosia leaf chloroform extract of table extracts flow
Aspergillus niger, black rot of pear bacterium, pears anthrax bacteria, Penicillium notatum, Pear black spot bacterium and Pyricularia oryzae are inoculated in PDA to consolidate
On body culture medium, dark culturing 5d at 25 DEG C of constant temperature, 0.5cm card punch punches the PDA for being paved with mycelia after mycelia uniformly grows
Culture medium, be inoculated in respectively containing 13.3,10,5,2.5,1,0.25,0.1, the PDA solids training of 3 extract of 0.05mg/mL embodiments
It supports on base.7d is cultivated at 25 DEG C and records inhibition zone, and bacteriostasis rate is as shown in table 7：
7. common rabdosia leaf chloroform extract bacteriostasis rate of table
It is found that common rabdosia leaf chloroform extract in 0.1mg/mL, has pears anthrax bacteria 62.7% bacteriostasis rate,
When 0.25mg/mL, bacteriostasis rate has had reached 100%；In 0.1mg/mL, just there is 100% bacteriostasis rate to Penicillium notatum；
When 0.1mg/mL, the bacteriostasis rate to Pear black spot bacterium is about 50%, reaches 100% in 13.3mg/mL；In 0.25mg/mL,
100% has been reached to the bacteriostasis rate of rice blast；In 0.05mg/mL, to the bacteriostasis rate of aspergillus niger just more than 50%, 0.25mg/
Reach 100% when mL；In 2.5mg/mL, just reach when just having reached 50%, 10mg/mL to the bacteriostasis rate of pears wheel line
100%.
Embodiment 4
Common rabdosia leaf leaf after drying is crushed on micronizer as after 200 mesh particles, with ethyl acetate according to 1kg:
The solid-liquid ratio of 3L (m/V) mixes, and carries out four stage Microwave Extractions, and detailed process is as shown in table 8：
8. common rabdosia leaf ethyl acetate extract of table extracts flow
Aspergillus niger, black rot of pear bacterium, pears anthrax bacteria, Penicillium notatum, Pear black spot bacterium and Pyricularia oryzae are inoculated in PDA to consolidate
On body culture medium, dark culturing 5d at 25 DEG C of constant temperature, 0.5cm card punch punches the PDA for being paved with mycelia after mycelia uniformly grows
Culture medium, be inoculated in respectively containing 13.3,10,5,2.5,1,0.25,0.1, the PDA solids training of 4 extract of 0.05mg/mL embodiments
It supports on base.7d is cultivated at 25 DEG C and records inhibition zone, and bacteriostasis rate is as shown in table 9：
9. common rabdosia leaf ethyl acetate extract bacteriostasis rate of table
It is found that common rabdosia leaf ethyl acetate extract in 13.3mg/mL, has pears anthrax bacteria 56.3% bacteriostasis rate；
In 5mg/mL, just there is 100% bacteriostasis rate to Penicillium notatum；In 5mg/mL, the bacteriostasis rate to Pear black spot bacterium is 100%；
In 1mg/mL, to the bacteriostasis rate about 50% of rice blast；In 0.05mg/mL, to the bacteriostasis rate of aspergillus niger just more than 50%,
Reach 100% when 0.1mg/mL；In 10mg/mL, 100% has just been reached to the bacteriostasis rate of pears wheel line.
As seen from the above embodiment, the common rabdosia leaf active constituent extracted using the method for the present invention is to agriculture disease fungus
Have certain restraining and sterilizing bacteria ability, the especially chloroform extract of common rabdosia leaf to aspergillus niger, black rot of pear bacterium, pears anthrax bacteria,
Penicillium notatum, Pear black spot bacterium and Pyricularia oryzae have 100% bacteriostasis rate.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of extracting method of common rabdosia leaf active constituent, includes the following steps：
1) after crushing common rabdosia leaf, according to 1kg：The mass volume ratio of (1~5) L is mixed with organic solvent, is carried out the first microwave and is carried
It takes；The power of first microwave is 50~200W, and the time of first microwave is 120~180s；
2) the second Microwave Extraction is carried out after step 1) first microwave, the power of second microwave is 45~50W, described the
The time of two microwaves is 350~450s；
3) third Microwave Extraction is carried out after step 2) second microwave, the power of the third microwave is 50~100W, described
The time of third microwave is 180~220s；
4) the 4th Microwave Extraction is carried out after step 3) the third microwave, the power of the 4th microwave is 40~50W, described the
The time of four microwaves is 550~650s, obtains common rabdosia leaf active constituent.
2. extracting method according to claim 1, which is characterized in that the grain size of the step 1) crushing is 170~230
Mesh.
3. extracting method according to claim 1, which is characterized in that the step 1) organic solvent includes saturated monohydroxy alcohol
Class, saturated ketone, esters or chloroform.
4. extracting method according to claim 1, which is characterized in that the temperature of step 1) first microwave is 40~70
℃。
5. extracting method according to claim 1, which is characterized in that the temperature of step 2) second microwave is 35~65
℃。
6. extracting method according to claim 1, which is characterized in that the temperature of step 3) the third microwave is 35~65
℃。
7. extracting method according to claim 1, which is characterized in that further including after step 4) the 4th microwave will be described
Obtained concentrate is dried in vacuo by extract concentrated by rotary evaporation.
8. the common rabdosia leaf active constituent extracted using claim 1~7 any one of them extracting method.
9. application of the common rabdosia leaf active constituent according to any one of claims 8 in inhibiting agriculture disease fungus activity.
10. application according to claim 9, which is characterized in that the agricultural disease fungus includes Pear black spot bacterium, pears wheel
Line germ, pears anthrax bacteria, Pyricularia oryzae, mould and aspergillus niger.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110393213A (en) * | 2019-09-04 | 2019-11-01 | 淮北师范大学 | The strawberry biological antimicrobial antistaling agent and preparation method of the extract containing common rabdosia leaf |
| CN110463725A (en) * | 2019-09-04 | 2019-11-19 | 淮北师范大学 | A kind of environment-friendly type coating agent for seed and preparation method |
| CN114903949A (en) * | 2022-06-08 | 2022-08-16 | 宿州市王枣子种植专业合作社 | A semen Ziziphi Spinosae extract for treating pulmonary nodule |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110393213A (en) * | 2019-09-04 | 2019-11-01 | 淮北师范大学 | The strawberry biological antimicrobial antistaling agent and preparation method of the extract containing common rabdosia leaf |
| CN110463725A (en) * | 2019-09-04 | 2019-11-19 | 淮北师范大学 | A kind of environment-friendly type coating agent for seed and preparation method |
| CN114903949A (en) * | 2022-06-08 | 2022-08-16 | 宿州市王枣子种植专业合作社 | A semen Ziziphi Spinosae extract for treating pulmonary nodule |
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