CN108426974B - Thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder - Google Patents
Thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder Download PDFInfo
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Abstract
The invention discloses a thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder, and relates to the technical field of medicinal material detection. The method comprises the following steps: preparing a ganoderma lucidum medicinal material solution and a ganoderma lucidum spore powder medicinal material solution, and detecting the ganoderma lucidum medicinal material by thin-layer chromatography: sucking the two solutions, respectively dropping on the same thin layer plate, developing with mixed solution of petroleum ether, ethyl acetate and formic acid as developing agent, taking out, air drying, developing, and inspecting under ultraviolet lamp; and (3) carrying out thin-layer chromatography detection on ganoderma lucidum spore powder: sucking the two solutions, respectively dropping on the same thin layer plate, developing with mixed solution of ethyl acetate, methanol, water and formic acid as developing agent, taking out, air drying, developing, and inspecting under ultraviolet lamp. The method is simple and convenient to operate, the main spots of the ganoderma lucidum and the ganoderma lucidum spore powder are clearly distinguished and cleared, the repeatability is good, the accuracy is high, and the popularization and the use are easy.
Description
Technical Field
The invention relates to the technical field of medicinal material detection, in particular to a thin-layer chromatography identification method of ganoderma lucidum and ganoderma lucidum spore powder.
Background
Ganoderma is dry fruiting body of Ganoderma lucidum or Ganoderma sinensis of Polyporaceae, has mild nature and sweet taste, and has effects of invigorating qi, tranquilizing mind, relieving cough and asthma, and can be used for treating restlessness of heart-mind, insomnia, cardiopalmus, lung deficiency, cough and asthma, asthenia, short breath, and anorexia. Ganoderma lucidum mainly contains triterpenes, ganoderan, nucleosides, steroids, etc. Ganoderma spore powder is dry mature spore of Ganoderma lucidum belonging to Polyporaceae, has effects of invigorating qi, tranquilizing mind, invigorating spleen, and benefiting lung, and can be used for regulating immunity, resisting tumor, regulating blood lipid, reducing blood sugar, and regulating nervous system and cardiovascular system; the main effective components of Ganoderma spore powder are Ganoderma polysaccharides and triterpenes.
In the existing method for identifying ganoderma lucidum thin-layer chromatography in pharmacopoeia of 2015 edition, main spots of the thin-layer chromatography of ganoderma lucidum and ganoderma lucidum spore powder are the same, and the ganoderma lucidum spore powder cannot be distinguished. While the reports of the literature of Pengjiang Li et al, the research on the identification of lucid ganoderma and confused hard-hole lucid ganoderma and the literature of Zhao Rui Shi et al, the research on the high performance liquid chromatography and the thin layer chromatography fingerprint of lucid ganoderma triterpenes are mainly thin layer chromatography methods of lucid ganoderma, no method for the thin layer chromatography comparative analysis of lucid ganoderma and lucid ganoderma spore powder exists, and the main spots of the lucid ganoderma and the lucid ganoderma spore powder are not clearly distinguished. Based on the above, it is particularly necessary to design a thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder, which is simple and convenient to operate, has the advantages of clear and definite spots for main distinguishing of ganoderma lucidum and ganoderma lucidum spore powder, good repeatability, high accuracy and easiness in popularization and use.
In order to achieve the purpose, the invention is realized by the following technical scheme: a thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder comprises the following steps:
(1) preparing a ganoderma medicinal material solution: taking 2g of ganoderma lucidum powder, adding 30ml of 95% ethanol, placing in a 100ml conical flask, extracting, filtering, evaporating filtrate in a water bath in an evaporating dish, and dissolving with 2ml of methanol for later use;
(2) preparing a ganoderma lucidum spore powder medicinal material solution: taking 2g of ganoderma spore powder, adding 30ml of 95% ethanol, placing in a 100ml conical flask, extracting, filtering, evaporating filtrate in a water bath in an evaporating dish, and dissolving with 2ml of methanol for later use;
(3) and (3) carrying out thin-layer chromatography detection on ganoderma lucidum: sucking 5ul of each of the two solutions in the steps (1) and (2), respectively dropping the two solutions on the same silica gel G high-efficiency thin-layer plate, taking a mixed solution of petroleum ether, ethyl acetate and formic acid as a developing agent, developing for 6cm, taking out, airing, spraying a 10% sulfuric acid ethanol solution, heating at 105 ℃ until spots are clearly developed, and placing under an ultraviolet lamp for inspection;
(4) and (3) carrying out thin-layer chromatography detection on ganoderma lucidum spore powder: sucking 5ul of each of the two solutions in the steps (1) and (2), respectively dropping on the same silica gel G high-efficiency thin-layer plate, taking a mixed solution of ethyl acetate, methanol, water and formic acid as a developing agent, developing for 6cm, taking out, drying in the air, spraying with a 10% sulfuric acid ethanol solution, heating at 105 ℃ until the spots are clearly developed, and placing under an ultraviolet lamp for inspection.
Preferably, the extraction in the steps (1) and (2) is ultrasonic extraction, and the extraction time is 30 min.
Preferably, the ratio of each component of the developing solvent in the step (3) is petroleum ether: ethyl acetate: formic acid = 1: 3: 0.1.
preferably, the developing solvent in the step (4) comprises the following components in percentage by weight: methanol: water: formic acid = 1: 1: 0.1: 0.1.
preferably, the wavelength of the ultraviolet lamp in the steps (3) and (4) is 365 nm.
The invention has the beneficial effects that: the method is simple and convenient to operate, the main spots of the ganoderma lucidum and the ganoderma lucidum spore powder are clearly distinguished and analyzed, the repeatability is good, and the accuracy is high.
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The invention is described in detail below with reference to the drawings and the detailed description;
FIG. 1 is a thin layer chromatogram of Ganoderma lucidum and Ganoderma lucidum spore powder under ultraviolet light.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Referring to fig. 1, the following technical solutions are adopted in the present embodiment: a thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder comprises the following steps:
(1) preparing a ganoderma medicinal material solution: taking 2g of Ganoderma powder, adding 30ml of 95% ethanol, placing in a 100ml conical flask, ultrasonically extracting for 30min, filtering, evaporating filtrate in evaporating dish with water bath, and dissolving with 2ml of methanol for use;
(2) preparing a ganoderma lucidum spore powder medicinal material solution: taking 2g of Ganoderma spore powder, adding 30ml of 95% ethanol, placing in a 100ml conical flask, ultrasonically extracting for 30min, filtering, evaporating the filtrate in evaporating dish with water bath, and dissolving with 2ml of methanol for use;
(3) and (3) carrying out thin-layer chromatography detection on ganoderma lucidum: sucking 5ul of each of the two solutions, and respectively dropping the two solutions on the same silica gel G high-efficiency thin-layer plate, wherein the weight ratio of petroleum ether: ethyl acetate: developing 6cm with formic acid (1: 3: 0.1) solution as developer, taking out, air drying, spraying 10% sulphuric acid ethanol solution, heating at 105 deg.C until the spots are clearly developed, and inspecting under ultraviolet lamp (365 nm);
(4) and (3) carrying out thin-layer chromatography detection on ganoderma lucidum spore powder: sucking 5ul of each of the 2 solutions, and respectively dropping the solution on the same silica gel G high-efficiency thin-layer plate, adding ethyl acetate: methanol: water: developing with formic acid (1: 1: 0.1: 0.1) solution as developer for 6cm, taking out, air drying, spraying 10% sulfuric acid ethanol solution, heating at 105 deg.C until the color of spots is clear, and inspecting under ultraviolet lamp (365 nm).
The specific implementation mode mainly aims at the thin-layer chromatography identification of the ganoderma lucidum and the ganoderma lucidum spore powder, can be used for the thin-layer chromatography identification of the ganoderma lucidum and the ganoderma lucidum spore powder in medicinal materials, extracts and traditional Chinese medicine compounds, and mainly distinguishes spots and is clear and clear.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. A thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder is characterized by comprising the following steps:
(1) preparing a ganoderma medicinal material solution: taking 2g of ganoderma lucidum powder, adding 30ml of 95% ethanol, placing in a 100ml conical flask, extracting, filtering, evaporating filtrate in a water bath in an evaporating dish, and dissolving with 2ml of methanol for later use;
(2) preparing a ganoderma lucidum spore powder medicinal material solution: taking 2g of ganoderma spore powder, adding 30ml of 95% ethanol, placing in a 100ml conical flask, extracting, filtering, evaporating filtrate in a water bath in an evaporating dish, and dissolving with 2ml of methanol for later use;
(3) and (3) carrying out thin-layer chromatography detection on ganoderma lucidum: sucking 5ul of each of the two solutions in the steps (1) and (2), respectively dropping the two solutions on the same silica gel G high-efficiency thin-layer plate, taking a mixed solution of petroleum ether, ethyl acetate and formic acid as a developing agent, developing for 6cm, taking out, airing, spraying a 10% sulfuric acid ethanol solution, heating at 105 ℃ until spots are clearly developed, and placing under an ultraviolet lamp for inspection;
(4) and (3) carrying out thin-layer chromatography detection on ganoderma lucidum spore powder: sucking 5ul of each of the two solutions in the steps (1) and (2), respectively dropping the two solutions on the same silica gel G high-efficiency thin-layer plate, taking a mixed solution of ethyl acetate, methanol, water and formic acid as a developing agent, developing for 6cm, taking out, airing, spraying a 10% sulfuric acid ethanol solution, heating at 105 ℃ until spots are clearly developed, and placing under an ultraviolet lamp for inspection;
the developing agent in the step (3) comprises the following components in percentage by weight: ethyl acetate: formic acid = 1: 3: 0.1;
the developing agent in the step (4) comprises the following components in percentage by weight: methanol: water: formic acid = 1: 1: 0.1: 0.1.
2. the thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder according to claim 1, wherein the extraction in the step (1) is ultrasonic extraction, and the extraction time is 30 min.
3. The thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder according to claim 1, wherein the extraction in the step (2) is ultrasonic extraction, and the extraction time is 30 min.
4. The thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder according to claim 1, wherein the wavelength of the ultraviolet lamp in the step (3) is 365 nm.
5. The thin-layer chromatography identification method for ganoderma lucidum and ganoderma lucidum spore powder according to claim 1, wherein the wavelength of the ultraviolet lamp in the step (4) is 365 nm.
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| CN110857938B (en) * | 2018-08-23 | 2022-03-11 | 完美（中国）有限公司 | Method for simultaneously identifying lucid ganoderma and vine of multiflower knotweed in traditional Chinese medicine composition |
| CN110857939B (en) * | 2018-08-23 | 2022-03-18 | 完美（中国）有限公司 | Detection method of traditional Chinese medicine composition with sleep improvement function |
| CN115078622A (en) * | 2022-05-07 | 2022-09-20 | 吉林省现代中药工程研究中心有限公司 | Method for identifying ganoderma lucidum spore powder in ganoderma lucidum spore powder compound product |
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| CA2414912A1 (en) * | 2001-12-20 | 2003-06-20 | University Of Saskatchewan Technologies Inc. | Pleurotus extract and use in treating hypertension |
| CN1261749C (en) * | 2003-12-03 | 2006-06-28 | 北京理工大学 | Method for rapidly and quantitatively determining the triterpenoid content in ganoderma lucidum |
| CN100515434C (en) * | 2005-05-30 | 2009-07-22 | 广州汉方现代中药研究开发有限公司 | Ganoderma lucidum spore oil, ganoderma lucidum spore powder and method for controlling quality of preparation of ganoderma lucidum |
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