CN107831261A - The quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation - Google Patents
The quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation Download PDFInfo
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- CN107831261A CN107831261A CN201711332334.9A CN201711332334A CN107831261A CN 107831261 A CN107831261 A CN 107831261A CN 201711332334 A CN201711332334 A CN 201711332334A CN 107831261 A CN107831261 A CN 107831261A
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Abstract
The present invention relates to a kind of quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation, the rhizoma alismatis root bark of tree peony root of herbaceous peony preparation is mainly prepared by the raw material of following parts by weight：19 21 parts of Fructus lycii P.E, 19 21 parts of radix paeoniae alba extraction, 14 16 parts of Cortex Moutan extract, 10 12 parts of Alisma extract；The quality determining method includes：The one or more in the Fructus lycii P.E, radix paeoniae alba extraction and Cortex Moutan extract, Alisma extract are detected using thin-layered chromatography.The quality determining method accurately, comprehensively can be monitored to the quality of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation, and easy to operate, high sensitivity, stability are good, can effectively control the product quality in rhizoma alismatis root bark of tree peony root of herbaceous peony formulation manufacturing processes, ensure product curative effect.
Description
Technical field
The present invention relates to traditional Chinese medicine quality detection, more particularly to the quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation.
Background technology
In recent years, with the development and growth in the living standard of national economy, the rhythm of life of modern is more and more faster, pressure
Power is also increasing, and as child's school work is nervous, young man's life is irregular, working clan's operating pressure is big to wait sub-health state all to hold
Immunity degradation is easily caused, and as China's the elderly's ratio is increased year by year, physical function declines, the old age of hypoimmunity
People is more and more, under this situation, has progressively turned into the body-building means of modern with food disease preventing and treating.
Rhizoma alismatis root bark of tree peony root of herbaceous peony capsule is the health food for having strengthen immunity function, helps solve modern's inferior health
And the problems such as physical function decline, and the quality standard that current rhizoma alismatis root bark of tree peony root of herbaceous peony capsule performs is more difficult accurate, comprehensive right
The quality of rhizoma alismatis root bark of tree peony root of herbaceous peony capsule is monitored.
The content of the invention
Based on this, it is necessary to provide a kind of quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation, the quality determining method energy
Enough accurately, comprehensively the quality of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation is monitored, and easy to operate, high sensitivity, stability are good, energy
Product quality in enough effectively control rhizoma alismatis root bark of tree peony root of herbaceous peony formulation manufacturing processes, ensures product curative effect.
A kind of quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation, the rhizoma alismatis root bark of tree peony root of herbaceous peony preparation is mainly by following parts by weight
Raw material be prepared：Fructus lycii P.E 19-21 parts, radix paeoniae alba extraction 19-21 parts, Cortex Moutan extract 14-16 parts, rhizoma alismatis
Extract 10-12 parts；
The quality determining method includes：Using thin-layered chromatography to the Fructus lycii P.E, radix paeoniae alba extraction and male
One or more in Cortex Moutan extract, Alisma extract are detected.
In one of the embodiments, the method detected to the Fructus lycii P.E comprises the following steps：
The Fructus lycii P.E, extracting in water are taken, gained extract solution is extracted with ethyl acetate, obtains need testing solution；
Fruit of Chinese wolfberry control medicinal material, extracting in water are taken, gained extract solution is extracted with ethyl acetate, obtains control medicinal material solution；
Draw the control medicinal material solution and need testing solution respectively, put on same silica gel thin-layer plate, using volume ratio as
14-16：7-9：The mixed liquor of 1-2 toluene, ethyl acetate and formic acid is solvent expansion, takes out, dries after expansion, in ultraviolet
Inspected under the conditions of lamp.
In one of the embodiments, in need testing solution or the control medicinal material solution preparation process, described plus water extraction
The method taken is：Every gram of Fructus lycii P.E adds water 100mL or fruit of Chinese wolfberry control medicinal material to add water 70mL, is heated to boiling and carries
15-25min is taken, is filtered after cooling, obtains extract solution.
In one of the embodiments, the wavelength of the uviol lamp is 365nm.
In one of the embodiments, the method detected to the radix paeoniae alba extraction comprises the following steps：
The radix paeoniae alba extraction is taken, adds ethanol to extract, after gained extract solution is evaporated, residue is dissolved with ethanol, obtains test sample
Solution；
Paeoniflorin reference substance is taken, adds ethanol to dissolve, obtains control medicinal material solution；
Draw the control medicinal material solution and need testing solution respectively, put on same silica gel thin-layer plate, using volume ratio as
39-41:4-6:9-11:0.1-0.5 chloroform, ethyl acetate, the mixed liquor of methanol and formic acid are solvent expansion, expansion
After take out, dry, with chromogenic reagent to clear spot, inspect.
In one of the embodiments, in the need testing solution preparation process, the method for described plus ethanol extraction is：Often
Gram radix paeoniae alba extraction adds ethanol 40mL, is ultrasonically treated 15-25min, filtration, obtains extract solution.
In one of the embodiments, the developer is the vanillin-sulfuric acid solution that mass concentration is 4-6%；It is described aobvious
The method of color is heating.
In one of the embodiments, the method detected to the Cortex Moutan extract comprises the following steps：
The Cortex Moutan extract is taken, adds ethanol to extract, after gained extract solution is evaporated, residue is dissolved with ethanol, is obtained for examination
Product solution；
Paeoniflorin reference substance is taken, adds ethanol to dissolve, obtains control medicinal material solution；
Draw the control medicinal material solution and need testing solution respectively, put on same silica gel thin-layer plate, using volume ratio as
39-41:4-6:9-11:0.1-0.5 chloroform, ethyl acetate, the mixed liquor of methanol and formic acid are solvent expansion, expansion
After take out, dry, with chromogenic reagent to clear spot, inspect.
In one of the embodiments, in the need testing solution preparation process, the method for described plus ethanol extraction is：Often
Gram Cortex Moutan extract adds ethanol 20mL, is ultrasonically treated 25-35min, filtration, obtains the extract solution.
In one of the embodiments, the developer is the vanillin-sulfuric acid solution that mass concentration is 4-6%；It is described aobvious
The method of color is heating.
In one of the embodiments, the method detected to the Alisma extract comprises the following steps：
The Alisma extract is taken, adds methanol to extract, after gained extract solution is evaporated, residue is dissolved with methanol, obtains test sample
Solution；
Alisol B monoacetate is taken, adds methanol to dissolve, obtains control medicinal material solution；
Draw the control medicinal material solution and need testing solution respectively, put on same silica gel thin-layer plate, using volume ratio as
3-5:1-3：The mixed liquor of 0.5-1.5 hexamethylene, ethyl acetate and acetone is solvent expansion, takes out, dries after expansion, with
Chromogenic reagent is inspected to clear spot.
In one of the embodiments, in the need testing solution preparation process, the method for described plus methanol extraction is：Often
Gram Alisma extract adds methanol 20mL, is ultrasonically treated 25-35min, filtration, obtains extract solution.
In one of the embodiments, the developer is the vanillic aldehyde solution that mass concentration is 0.4-0.6%, and solvent is
Volume ratio is 3-5:1 sulfuric acid and the mixed liquor of ethanol；The method of the colour developing is in 100-110 DEG C of heating.
In one of the embodiments, the quality determining method：Also include red to the rhizoma alismatis using thin-layered chromatography
Skin root of herbaceous peony preparation is detected：
The rhizoma alismatis root bark of tree peony root of herbaceous peony preparation is taken, adds methanol to extract, after gained extract solution is evaporated, residue is dissolved with methanol, is obtained
Need testing solution；
Alisol B monoacetate is taken, adds methanol to dissolve, obtains control medicinal material solution；
Draw the control medicinal material solution and need testing solution respectively, put on same silica gel thin-layer plate, using volume ratio as
3-5:1-3：The mixed liquor of 0.5-1.5 hexamethylene, ethyl acetate and acetone is solvent expansion, takes out, dries after expansion, with
Chromogenic reagent is inspected to clear spot.
In one of the embodiments, in the need testing solution preparation process, the method for described plus methanol extraction is：Often
Gram rhizoma alismatis root bark of tree peony root of herbaceous peony preparation adds methanol 8mL, is ultrasonically treated 25-35min, filtration, obtains extract solution.
In one of the embodiments, the developer is the vanillic aldehyde solution that mass concentration is 0.4-0.6%, and solvent is
Volume ratio is 3-5:1 sulfuric acid and alcohol mixeding liquid；The method of the colour developing is in 100-110 DEG C of heating.
Compared with prior art, the invention has the advantages that：
The quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation of the present invention, by rationally controlling testing conditions, effectively
One or more in Fructus lycii P.E in prescription, radix paeoniae alba extraction and Cortex Moutan extract, Alisma extract are carried out
Thin layer discriminating, further the raw material for preparing rhizoma alismatis root bark of tree peony root of herbaceous peony preparation can be differentiated, carried on the basis of current standard
High, the perfect quality standard of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation, and this method is easy to operate, high sensitivity, stability are good, Neng Gouyou
Product quality in effect control rhizoma alismatis root bark of tree peony root of herbaceous peony formulation manufacturing processes, ensures product curative effect.
Further, the quality determining method also determines to be used as comparison medicine using Alisol B monoacetate by largely testing
Material coordinates certain solvent system to detect rhizoma alismatis root bark of tree peony root of herbaceous peony formulation products, can with reference to the discriminating of above-mentioned raw materials
The comprehensive quality of production standard for improving rhizoma alismatis root bark of tree peony root of herbaceous peony preparation, ensure the quality and curative effect of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation.
Brief description of the drawings
Fig. 1 is indentification by TLC figure (its of the radix paeoniae alba extraction in one embodiment of the invention rhizoma alismatis root bark of tree peony root of herbaceous peony capsule
In, 1：Negative control；2：Paeoniflorin reference substance solution；3：Radix paeoniae alba extraction is 1.；4：Radix paeoniae alba extraction is 2.；5：Radix paeoniae alba extraction
③)；
Fig. 2 is the indentification by TLC figure (wherein, 1 of the Fructus lycii P.E in rhizoma alismatis root bark of tree peony root of herbaceous peony capsule described in Fig. 1：
Negative control；2：Fruit of Chinese wolfberry control medicinal material solution；3：Fructus lycii P.E is 1.；4：Fructus lycii P.E is 2.；5：The fruit of Chinese wolfberry extracts
Thing is 3.)；
Fig. 3 is the indentification by TLC figure (wherein, 1 of the Cortex Moutan extract in rhizoma alismatis root bark of tree peony root of herbaceous peony capsule described in Fig. 1：
Negative control；2：Paeoniflorin reference substance solution；3：Cortex Moutan extract is 1.；4：Cortex Moutan extract is 2.；5：Cortex Moutan extract
③)；
Fig. 4 is the indentification by TLC figure (1 of the Alisma extract in rhizoma alismatis root bark of tree peony root of herbaceous peony capsule described in Fig. 1：It is negative right
According to；2：Alisol B monoacetate reference substance solution；3：Alisma extract is 1.；4：Alisma extract is 2.；5：Alisma extract is 3.)；
Fig. 5 is the indentification by TLC figure (1 of rhizoma alismatis root bark of tree peony root of herbaceous peony capsule described in Fig. 1：Negative control；2：23- acetyl rhizoma alismatis
Alcohol B reference substance solutions；3：Rhizoma alismatis root bark of tree peony root of herbaceous peony capsule 's content is 1.；4：Rhizoma alismatis root bark of tree peony root of herbaceous peony capsule 's content is 2.；5：Rhizoma alismatis is red
Skin root of herbaceous peony capsule 's content is 3.).
Embodiment
The quality determining method of the rhizoma alismatis root bark of tree peony root of herbaceous peony preparation of the present invention is made below in conjunction with specific embodiment further detailed
Thin explanation.
The medicine and reagent that the embodiment of the present invention uses：
Fructus lycii P.E：Guangdong Xin Bao medicine companies Science and Technology Ltd., lot number：Z170901、Z170902、Z170903；
Fruit of Chinese wolfberry control medicinal material：National Institute for Food and Drugs Control, lot number：121072-201410；
Radix paeoniae alba extraction：Guangdong Xin Bao medicine companies Science and Technology Ltd., lot number：Z170901、Z170902、Z170903；
Paeoniflorin reference substance：National Institute for Food and Drugs Control, lot number：110736-201539；
Cortex Moutan extract：Guangdong Xin Bao medicine companies Science and Technology Ltd., lot number：Z170901、Z170902、Z170903；
Alisma extract：Guangdong Xin Bao medicine companies Science and Technology Ltd., lot number：Z170901、Z170902、Z170903；
Alisol B monoacetate：National Institute for Food and Drugs Control, lot number：111846-201504；
Rhizoma alismatis root bark of tree peony root of herbaceous peony capsule：Guangdong Xin Bao medicine companies Science and Technology Ltd., lot number：Z20171001、Z20171002、
Z20171003。
Embodiment 1
A kind of quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony capsule of the present embodiment, the content of the rhizoma alismatis root bark of tree peony root of herbaceous peony capsule
It is made up of following raw materials according according to following parts by weight：20 parts of Fructus lycii P.E, 20 parts of radix paeoniae alba extraction, Cortex Moutan extract 15
Part, 11 parts of Alisma extract, 2 parts of talcum powder, 2 parts of starch.
Quality determining method is directed to the Fructus lycii P.E being expected with content, radix paeoniae alba extraction, moutan bark extraction
Thing and Alisma extract are detected, and step is as follows：
(1) radix paeoniae alba extraction detects：This product 0.5g is taken, adds ethanol 20mL, is ultrasonically treated 20 minutes, filtration is evaporated, residue
Ethanol 1mL is added to make dissolving, as need testing solution.Paeoniflorin reference substance separately is taken, adds ethanol that solution of every lmL containing lmg is made, is made
For reference substance solution.Tested according to thin-layered chromatography (general rule 0502), draw each 5uL of above two solution, put respectively in same silicon
On glue G lamellaes, with chloroform-acetate-methanol-formic acid (volume ratio 40：5：10：0.2) it is solvent, expansion, takes
Go out, dry, spray with 5% vanillin-sulfuric acid solution, it is clear to be heated to spot development.As shown in figure 1, in test sample chromatogram, with
On the corresponding position of reference substance chromatogram, show the spot of same color.
(2) Fructus lycii P.E detects：This product 0.5g is taken, adds water 50mL, heating is boiled 20 minutes, let cool, and is filtered, filtrate
Shaken and extracted with ethyl acetate 20mL, divided and take acetic acid ethyl fluid, lmL is concentrated into, as need testing solution.Separately the fruit of Chinese wolfberry is taken to compare
Medicinal material 0.5g, add water 35mL, heating is boiled 15 minutes, let cool, and is filtered, and filtrate is shaken with ethyl acetate 15mL and extracted, and is divided and is taken second
Acetoacetic ester liquid, is concentrated into lmL, as control medicinal material solution.Tested according to thin-layered chromatography (general rule 0502), it is molten to draw above two
Each 10uL of liquid, put respectively on same silica gel g thin-layer plate, making into strips, with toluene-ethyl acetate-formic acid (volume ratio 15：8：
1.5) it is solvent, deploys, takes out, dry, put and inspected under ultraviolet lamp (365nm).As shown in Fig. 2 in test sample chromatogram,
On position corresponding with control medicinal material chromatogram, show the fluorescence spot of same color.
(3) Cortex Moutan extract detects：This product powder lg is taken, adds ethanol 20mL, is ultrasonically treated 30 minutes, filtration is evaporated,
Residue adds ethanol 2mL to make dissolving, as need testing solution.Paeoniflorin reference substance separately is taken, adds ethanol that every lmL is made containing the molten of lmg
Liquid, as reference substance solution.Tested according to thin-layered chromatography (general rule 0502), draw each 5uL of above two solution, put respectively in same
On one silica gel g thin-layer plate, with chloroform-acetate-methanol-formic acid (volume ratio 40：5：10：0.2) it is solvent, exhibition
Open, take out, dry, spray with 5% vanillin-sulfuric acid solution, it is clear to be heated to spot development.As shown in figure 3, in test sample chromatogram,
On position corresponding with reference substance chromatogram, show the spot of same color.
(4) Alisma extract detects：This product powder 1g is taken, adds methanol 20mL, is ultrasonically treated 30 minutes, filtration, filtrate is steamed
Dry, residue adds methanol 2mL to make dissolving, as need testing solution.Alisol B monoacetate reference substance separately is taken, adds methanol to be made often
Solution of the lmL containing 2mg, as reference substance solution.It is each that above two solution is drawn according to thin-layered chromatography (general rule 0502) experiment L
4uL, put respectively on same silica GF254 lamellae, with cyclohexane-ethyl acetate-acetone (volume ratio 4：2：1) it is solvent
Expansion, taking-up are dried, and spray 0.5% vanillin-sulfuric acid-ethanol (volume ratio 4：1) mixed liquor, it is clear to be heated to spot development at 105 DEG C
It is clear.As shown in figure 4, in test sample chromatogram, on position corresponding with reference substance chromatogram, show the spot of same color.
Using the quality determining method of embodiment 1 respectively to three batches of (Z170901, Z170902, Z170903) rhizoma alismatis root barks of tree peony
The raw material of root of herbaceous peony capsule is detected, and as Figure 1-4, Rf values are as follows for indentification by TLC figure：
The Rf values of radix paeoniae alba extraction in Fig. 1：1. number:0.4th, 2. number:0.4th, 3. number:0.4；
The Rf values of Fructus lycii P.E in Fig. 2：1. number:0.5th, 2. number:0.5th, 3. number:0.5；
The Rf values of Cortex Moutan extract in Fig. 3：1. number:0.4th, 2. number:0.4th, 3. number:0.4；
The Rf values of Alisma extract in Fig. 4：1. number:0.6th, 2. number:0.6th, 3. number:0.6.
It follows that good separating effect between each test point, Rf values are suitable, and reproducible, can be effectively from raw material
The product quality of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation is controlled, ensures product curative effect.
Embodiment 2
A kind of quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony capsule of the present embodiment, the rhizoma alismatis root bark of tree peony root of herbaceous peony capsule is (with implementation
1 same lot number of example) content be made up of following raw materials according according to following parts by weight：20 parts of Fructus lycii P.E, radix paeoniae alba extraction
20 parts, 15 parts of Cortex Moutan extract, 11 parts of Alisma extract, 2 parts of talcum powder, 2 parts of starch.
Quality determining method is detected for content, and step is as follows：
Content powder 5g is taken, adds methanol 40mL, is ultrasonically treated 30 minutes, filtration, filtrate is evaporated, and residue adds methanol 2mL
Make dissolving, as need testing solution.Alisol B monoacetate reference substance separately is taken, adds methanol that every lm is made, the solution containing 2mg, is made
For reference substance solution.Tested according to thin-layered chromatography (general rule 0502), draw each 4uL of above two solution, put respectively in same silicon
On glue GF254 lamellaes, with cyclohexane-ethyl acetate-acetone (volume ratio 4：2：1) it is solvent, deploys, take out, dry, sprays
0.5% vanillin-sulfuric acid-ethanol (volume ratio 4：1) solution, it is clear to be heated to spot development at 105 DEG C.As shown in figure 5, for examination
In product chromatogram, on position corresponding with reference substance chromatogram, show the spot of same color.
Using the quality determining method of embodiment respectively to three batches of (Z20171001, Z20171002, Z20171003) rhizoma alismatis
The content of root bark of tree peony root of herbaceous peony capsule is detected, and indentification by TLC figure is as shown in figure 5, Rf values are as follows：
The Rf values of rhizoma alismatis root bark of tree peony root of herbaceous peony capsule 's content in Fig. 5：1. number:0.5th, 2. number:0.6th, 3. number:0.6.
As can be seen here, good separating effect between each test point, Rf values are suitable, and reproducible, can be effectively from finished product control
The quality of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation processed, the raw material testing result in integrated embodiment 1, can effectively ensure that product curative effect.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope that this specification is recorded all is considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that come for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
- A kind of 1. quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation, it is characterised in that the rhizoma alismatis root bark of tree peony root of herbaceous peony preparation mainly by The raw material of following parts by weight is prepared：Fructus lycii P.E 19-21 parts, radix paeoniae alba extraction 19-21 parts, Cortex Moutan extract 14-16 parts, Alisma extract 10-12 parts；The quality determining method includes：The Fructus lycii P.E, radix paeoniae alba extraction, moutan bark are carried using thin-layered chromatography The one or more in thing, Alisma extract are taken to be detected.
- 2. the quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation according to claim 1, it is characterised in that to the matrimony vine The method that seed extract is detected comprises the following steps：The Fructus lycii P.E, extracting in water are taken, gained extract solution is extracted with ethyl acetate, obtains need testing solution；Fruit of Chinese wolfberry control medicinal material, extracting in water are taken, gained extract solution is extracted with ethyl acetate, obtains control medicinal material solution；The control medicinal material solution and need testing solution are drawn respectively, are put on same silica gel thin-layer plate, using volume ratio as 14- 16：7-9：The mixed liquor of 1-2 toluene, ethyl acetate and formic acid is solvent expansion, takes out, dries after expansion, in uviol lamp Under the conditions of inspect.
- 3. the quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation according to claim 2, it is characterised in that the test sample In solution or control medicinal material solution preparation process, the method for the extracting in water is：Every gram of Fructus lycii P.E adds water 100mL or fruit of Chinese wolfberry control medicinal material add water 70mL, are heated to boiling extraction 15-25min, are filtered after cooling, obtain the extract solution.
- 4. the quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation according to claim 1, it is characterised in that to the root of herbaceous peony The method that extract is detected comprises the following steps：The radix paeoniae alba extraction is taken, adds ethanol to extract, after gained extract solution is evaporated, residue is dissolved with ethanol, obtains need testing solution；Paeoniflorin reference substance is taken, adds ethanol to dissolve, obtains control medicinal material solution；The control medicinal material solution and need testing solution are drawn respectively, are put on same silica gel thin-layer plate, using volume ratio as 39- 41:4-6:9-11:0.1-0.5 chloroform, ethyl acetate, the mixed liquor of methanol and formic acid are solvent expansion, after expansion Take out, dry, with chromogenic reagent to clear spot, inspect.
- 5. the quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation according to claim 4, it is characterised in that the test sample In solution preparation process, the method for described plus ethanol extraction is：Every gram of radix paeoniae alba extraction adds ethanol 40mL, is ultrasonically treated 15-25min, filtration, obtains the extract solution.
- 6. the quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation according to claim 1, it is characterised in that to the tree peony The method that bark extract is detected comprises the following steps：The Cortex Moutan extract is taken, adds ethanol to extract, after gained extract solution is evaporated, residue is dissolved with ethanol, and it is molten to obtain test sample Liquid；Paeoniflorin reference substance is taken, adds ethanol to dissolve, obtains control medicinal material solution；The control medicinal material solution and need testing solution are drawn respectively, are put on same silica gel thin-layer plate, using volume ratio as 39- 41:4-6:9-11:0.1-0.5 chloroform, ethyl acetate, the mixed liquor of methanol and formic acid are solvent expansion, after expansion Take out, dry, with chromogenic reagent to clear spot, inspect.
- 7. the quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation according to claim 6, it is characterised in that the test sample In solution preparation process, the method for described plus ethanol extraction is：Every gram of Cortex Moutan extract adds ethanol 20mL, is ultrasonically treated 25-35min, filtration, obtains the extract solution.
- 8. the quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation according to claim 1, it is characterised in that to the rhizoma alismatis The method that extract is detected comprises the following steps：The Alisma extract is taken, adds methanol to extract, after gained extract solution is evaporated, residue is dissolved with methanol, obtains need testing solution；Alisol B monoacetate is taken, adds methanol to dissolve, obtains control medicinal material solution；The control medicinal material solution and need testing solution are drawn respectively, are put on same silica gel thin-layer plate, using volume ratio as 3-5: 1-3：The mixed liquor of 0.5-1.5 hexamethylene, ethyl acetate and acetone is solvent expansion, takes out, dries after expansion, with colour developing Agent develops the color to clear spot, inspects.
- 9. the quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation according to claim 8, it is characterised in that the test sample In solution preparation process, the method for described plus methanol extraction is：Every gram of Alisma extract adds methanol 20mL, is ultrasonically treated 25-35min, filtration, obtains the extract solution.
- 10. the quality determining method of the rhizoma alismatis root bark of tree peony root of herbaceous peony preparation according to claim any one of 1-9, it is characterised in that The quality determining method：Also include detecting the rhizoma alismatis root bark of tree peony root of herbaceous peony preparation using thin-layered chromatography：The rhizoma alismatis root bark of tree peony root of herbaceous peony preparation is taken, adds methanol to extract, after gained extract solution is evaporated, residue is dissolved with methanol, is obtained for examination Product solution；Alisol B monoacetate is taken, adds methanol to dissolve, obtains control medicinal material solution；The control medicinal material solution and need testing solution are drawn respectively, are put on same silica gel thin-layer plate, using volume ratio as 3-5: 1-3：The mixed liquor of 0.5-1.5 hexamethylene, ethyl acetate and acetone is solvent expansion, takes out, dries after expansion, with colour developing Agent develops the color to clear spot, inspects.
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| CN111638300A (en) * | 2020-04-26 | 2020-09-08 | 广东经典名方科技有限公司 | Thin-layer identification method for 16-oxo-alisol A in alisma orientale |
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