Medical science bacterium multistage separation screening system
Technical Field
The invention belongs to the technical field of microbial detection, and particularly relates to a medical bacteria multistage separation screening system.
Background
The isolation of bacteria refers to a process of separating specific bacteria from a mixture of bacteria and cells, or a mixture of various bacteria, and is very important in clinical medicine, food industry, water quality testing, bioengineering, and pharmaceutical industry. Traditional bacteria isolation is carried out in agar culture medium, the culture condition is harsh and time-consuming, and the isolation culture process is easily polluted by mixed bacteria, so the bacteria isolation culture based on the method can not obtain ideal results. The existing microbial separator can only carry out primary separation, the separation is not thorough, the requirement of microbial separation cannot be met, the experimental requirement cannot be met, and the scientific research requirement cannot be met.
The prior art also discloses a technical scheme for bacteria separation, for example, a chinese patent with application number 201620075181 discloses a medical microorganism two-stage separation screening device, which comprises a frame, a universal wheel, a microorganism cylinder, a filtering cylinder, a second-stage centrifugal cylinder, a first-stage centrifugal cylinder, a mounting plate, a rotating shaft, a driven pulley, a driving pulley, a belt, a motor, a sealing gasket i, a sealing gasket ii, a centrifugal cylinder cover, an inner pad, a top cover, a handle and an outlet, wherein the microorganism cylinder is connected to the top end of the frame. The driving belt pulley is arranged on an output shaft of the motor. The driven pulley fixed connection is at the lower extreme of pivot, and the belt suit is on driven pulley and drive pulley. The upper end of the rotating shaft penetrates through the microorganism cylinder and the top end of the filtering cylinder to be fixedly connected with the mounting plate. The secondary centrifugal cylinder and the primary centrifugal cylinder are fixedly connected onto the mounting plate through screws, the centrifugal cylinder cover is clamped at the top ends of the secondary centrifugal cylinder and the primary centrifugal cylinder, the inner gasket is arranged between the microbial cylinder body and the top cover, the filtering cylinder body is sleeved on the rotating shaft, the sealing gasket I is arranged between the microbial cylinder body and the rotating shaft, and the sealing gasket II is arranged between the filtering cylinder body and the rotating shaft. Although the technical scheme can realize the separation of bacteria through two-stage screening, the rotating speed is the same when the two-stage centrifugation is carried out, more energy sources are consumed for achieving an ideal effect, meanwhile, the vertically arranged filter screen cannot be fully utilized, the working efficiency is low, and the use of the invention is limited.
In view of this, the medical bacteria multistage separation and screening system provided by the invention is used for centrifugally screening bacteria by setting multistage centrifugation with different rotating speeds, and has the advantages of good centrifugation effect and high working efficiency.
Disclosure of Invention
In order to make up the defects of the prior art, the invention provides a medical bacteria multistage separation and screening system which is mainly used for realizing efficient separation and screening of bacteria through multistage centrifugation. According to the invention, through the mutual matching of the centrifugal module and the transmission module, the bacteria liquid can be screened by multi-stage differential centrifugation, and the centrifugation effect is good.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a medical bacteria multistage separation screening system which comprises a box body, a centrifugal module, a transmission module and a vibration module, wherein the box body is of a cuboid structure with a built-in cavity, the box body is arranged on the ground through support legs, a feeding hole is formed in the top of the box body, and a discharging hole is formed in the bottom of the box body; the centrifugal module is positioned in the cavity of the box body and is used for gradually centrifuging the bacterial liquid to realize the separation and screening of bacteria; the transmission module is positioned on one side of the centrifugal module and is used for realizing the differential rotation of the centrifugal module; the vibration module is arranged on the centrifugal module and used for avoiding blockage of the centrifugal module.
The centrifugal module comprises a centrifugal cylinder, a screen and a spring I, wherein annular chutes are formed in the lower surface and the bottom of the top of the box body, the centrifugal cylinder is of a round platform structure, round platform-shaped through holes are coaxially formed in the round platform, the upper bottom surface of the round platform is smaller than the lower bottom surface of the round platform, the number of the centrifugal cylinders is at least three, the centrifugal cylinders are vertically placed, annular protrusions are arranged on the lower bottom surface of the centrifugal cylinder, annular grooves are correspondingly formed in the upper bottom surface of the centrifugal cylinder, the upper centrifugal cylinder is installed in the annular grooves of the lower centrifugal cylinder through the annular protrusions to be connected, the centrifugal cylinders can rotate relatively, the upper end of the uppermost centrifugal cylinder is located in the annular chute of the box body, the lower end of the lowermost centrifugal cylinder is located in the annular chute of; the screen cloth quantity is the same with centrifuge bowl quantity, and the screen cloth passes through spring one horizontal installation inside centrifuge bowl, and the aperture of top screen cloth is greater than the aperture of below screen cloth. When the centrifugal screen is used, the centrifugal cylinder rotates to drive the screen to rotate, the rotating speed of the centrifugal cylinder at the top is fastest, the centrifugal effect of the first-stage centrifugation can be improved, the aperture of the screen mesh is gradually reduced from top to bottom, the bacterial liquid is purified through three-stage centrifugation, and the purified bacteria fall to the bottom of the box body.
Still be provided with the guide board on the centrifuge bowl inner wall, guide board slope is installed on the centrifuge bowl inner wall, and the fungus liquid of adhesion on the centrifuge bowl inner wall falls back in the sieve of below when the guide board is used for the centrifugation. When the centrifugal cylinder rotates, the bacteria liquid part passing through the screen is thrown onto the inner wall of the centrifugal cylinder, the bacteria liquid slides downwards along the inner wall of the centrifugal cylinder, and the bacteria liquid enters the screen below along the guide plate due to the existence of the guide plate, so that the centrifugal effect is improved.
The transmission module comprises bevel gears, transmission shafts, a transmission motor and a bracket, the number of the bevel gears is the same as that of the centrifugal cylinders, the bevel gears are coaxially arranged on the vertical transmission shafts, the bevel gears above the vertical transmission shafts are larger than those below the vertical transmission shafts, and the bevel gears are meshed with a tooth-shaped structure on the outer surface of the centrifugal cylinders; the transmission motor is arranged on the support legs of the box body through a support, and an output shaft of the transmission motor is connected with the transmission shaft. When the centrifugal machine is used, the transmission motor drives the bevel gears to coaxially rotate, the bevel gears rotate to enable the centrifugal cylinders meshed with the bevel gears to synchronously rotate, and the rotating speeds of the centrifugal cylinders are increased step by step from bottom to top due to the fact that the bevel gears are different in size.
The vibration module comprises a knocking block, a blocking rod, a stop block and a second spring, and the knocking block is arranged at the bottom of the screen; the blocking rod is arranged on the lower screen mesh, a sliding groove is vertically formed in the upper surface of the blocking rod, which is positioned above the lower screen mesh, of the blocking rod, and a second spring is arranged in the sliding groove; the stop dog is located the sliding tray of stopping the pole, and spring two is connected with the stop dog, and the stop dog height is higher than the height of strikeing the piece, and the contact surface of stop dog and the piece of strikeing is the inclined plane, and it is arc with the stop dog contact position to strike the piece. Because there is the rotational speed difference between the centrifugal cylinder adjacent downwards, the centrifugal cylinder rotational speed of top is higher than the rotational speed of below centrifugal cylinder, strike the piece and move and take place the striking when contacting with the dog, the impact makes the screen cloth produce the vibration, improve centrifugal effect, the dog receives to strike the piece and strike the back and remove downwards along the sliding tray that blocks the pole, make and strike the piece and pass through the dog smoothly, strike the piece and break away from the contact back with the dog, the dog resumes original position under the spring action of spring two, prepare for the striking of next time.
The box body is provided with a plugging module at the position of the discharge port, a disc-shaped groove is horizontally arranged in the box body at the position of the discharge port, the diameter of the disc-shaped groove is larger than that of the discharge port, and the center of the disc-shaped groove deviates from the discharge port; the plugging module comprises a plugging plate, a rotating shaft and a plugging motor, wherein the plugging plate is located in a disc-shaped groove in the bottom of the box body, a through hole is eccentrically formed in the plugging plate, the plugging plate is rotated through the rotating shaft and the plugging motor, the through hole in the plugging plate is rotated to a position coaxial with the discharge hole, and the collection of bacteria after separation is realized. When the bacteria falling to the bottom of the box body after purification need to be collected, the plugging motor drives the rotating shaft to rotate so as to enable the plugging plate to move, the through hole in the plugging plate is rotated to the position coaxial with the discharge port by the movement of the plugging plate, and the bacteria liquid smoothly flows out through the discharge port.
The invention has the beneficial effects that:
1. According to the medical bacteria multistage separation screening system, the centrifugal module, the transmission module and the vibration module are matched with each other to work, and the bevel gear is used for achieving differential operation of the centrifugal cylinder, so that multistage differential centrifugation is achieved, and the bacteria separation effect and the energy utilization are improved; meanwhile, the vibration module realizes regular knocking on the screen by utilizing the differential rotation of the centrifugal cylinder, so that the blockage of the screen is avoided.
2. According to the medical bacteria multistage separation screening system, the plugging module at the bottom of the box body is provided with the eccentric through hole on the plugging plate, the plugging plate rotates to close and open the discharge hole of the box body, the reliability is high, the sealing performance is good, and the bacterial pollution is avoided.
3. according to the medical bacteria multistage separation screening system, the vibration module is further arranged on the screen, the knocking block and the stop block are respectively arranged between the upper screen and the lower screen, the upper screen regularly knocks the lower screen by utilizing the differential motion between the screens, so that the screens vibrate, the screens are prevented from being blocked, and the centrifugal effect is improved.
Drawings
the invention will be further explained with reference to the drawings.
FIG. 1 is a front view of the present invention;
3 FIG. 32 3 is 3 a 3 cross 3- 3 sectional 3 view 3 A 3- 3 A 3 of 3 FIG. 31 3 of 3 the 3 present 3 invention 3; 3
FIG. 3 is a cross-sectional view B-B of FIG. 1 of the present invention;
In the figure: the device comprises a box body 1, a centrifugal module 2, a transmission module 3, a cleaning module 4, support legs 11, a centrifugal cylinder 21, a screen 22, a guide plate 23, a bevel gear 31, a transmission shaft 32, a transmission motor 33, a vibration module 4, a knocking block 41, a blocking rod 42, a blocking block 43, a blocking module 5, a blocking plate 51 and a blocking motor 52.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
as shown in fig. 1 to 3, the medical bacteria multistage separation screening system comprises a box body 1, a centrifugal module 2, a transmission module 3 and a vibration module 4, wherein the box body 1 is of a cuboid structure with a built-in cavity, the box body 1 is mounted on the ground through support legs 11, a feed inlet is formed in the top of the box body 1, and a discharge outlet is formed in the bottom of the box body 1; the centrifugal module 2 is positioned in the cavity of the box body 1, and the centrifugal module 2 is used for gradually centrifuging the bacterial liquid to realize separation and screening of bacteria; the transmission module 3 is positioned at one side of the centrifugal module 2, and the transmission module 3 is used for realizing the differential rotation of the centrifugal module 2; the vibration module 4 is arranged on the centrifugal module 2, and the vibration module 4 is used for avoiding the blockage of the centrifugal module 2.
The centrifugal module 2 comprises a centrifugal cylinder 21, a screen 22 and a first spring, annular sliding grooves are formed in the lower surface and the bottom of the top of the box body 1, the centrifugal cylinder 21 is of a circular table structure, circular table type through holes are coaxially formed in the circular table, the upper bottom surface of the circular table is smaller than the lower bottom surface of the circular table, at least three centrifugal cylinders 21 are arranged, the centrifugal cylinders 21 are vertically placed, annular protrusions are arranged on the lower bottom surface of the centrifugal cylinder 21, annular grooves are correspondingly formed in the upper bottom surface of the centrifugal cylinder 21, the upper centrifugal cylinder 21 is installed in the annular grooves of the lower centrifugal cylinder 21 through the annular protrusions to achieve connection, the centrifugal cylinders 21 can rotate relatively, the upper end of the uppermost centrifugal cylinder 21 is located in the annular sliding grooves of the box body 1, the lower end of the lowermost centrifugal cylinder 21 is located in the annular sliding grooves of the bottom of the; the number of the screens 22 is the same as that of the centrifuge tube 21, the screens 22 are horizontally arranged in the centrifuge tube 21 through a spring I, and the aperture of the upper screen 22 is larger than that of the lower screen 22. During the use, centrifuge bowl 21 rotates and drives the screen cloth 22 and rotate, and the top centrifuge bowl 21 rotational speed is the fastest, can be to improving the centrifugal effect of first order, and screen cloth 22 aperture from last down reduces gradually, and the fungus liquid is through three-stage centrifugation purification, and the bacterium after the purification falls to box 1 bottom.
Still be provided with guide plate 23 on the centrifuge bowl 21 inner wall, guide plate 23 slope is installed on centrifuge bowl 21 inner wall, and guide plate 23 is used for the bacterium liquid of adhesion on centrifuge bowl 21 inner wall to fall back in the screen cloth 22 of below when centrifuging. When the centrifuge bowl 21 rotates, the bacteria liquid passing through the screen 22 is thrown onto the inner wall of the centrifuge bowl 21, the bacteria liquid slides down along the inner wall of the centrifuge bowl 21, and the bacteria liquid enters the screen 22 below along the guide plate 23 due to the existence of the guide plate 23, so that the centrifugation effect is improved.
the transmission module 3 comprises bevel gears 31, transmission shafts 32, a transmission motor 33 and a support, the number of the bevel gears 31 is the same as that of the centrifugal cylinders 21, the bevel gears 31 are coaxially arranged on the vertical transmission shafts 32, the bevel gear 31 above is larger than the bevel gear 31 below, and the bevel gears 31 are meshed with the tooth-shaped structure on the outer surface of the centrifugal cylinder 21; the transmission motor 33 is arranged on the support leg 11 of the box body 1 through a bracket, and an output shaft of the transmission motor 33 is connected with the transmission shaft 32. When the centrifugal machine is used, the transmission motor 33 drives the bevel gear 31 to rotate coaxially, the bevel gear 31 rotates to enable the centrifugal cylinders 21 meshed with the bevel gear 31 to rotate synchronously, and the rotating speed of each centrifugal cylinder 21 is increased step by step from bottom to top due to the fact that the bevel gear 31 is different in size.
The vibration module 4 comprises a knocking block 41, a blocking rod 42, a stop block 43 and a second spring, wherein the knocking block 41 is arranged at the bottom of the screen 22; the blocking rod 42 is arranged on the lower screen 22, a sliding groove is vertically formed in the upper surface of the blocking rod 42, which is positioned above the lower screen 22, of the blocking rod 42, and a second spring is arranged in the sliding groove; the stop block 43 is positioned in the sliding groove of the stop rod 42, the second spring is connected with the stop block 43, the height of the stop block 43 is higher than that of the knocking block 41, the contact surface of the stop block 43 and the knocking block 41 is an inclined surface, and the contact position of the knocking block 41 and the stop block 43 is in an arc shape. Because the rotating speed difference exists between the downward adjacent centrifugal cylinders 21, the rotating speed of the centrifugal cylinder 21 above is higher than that of the centrifugal cylinder 21 below, the knocking block 41 collides when moving to contact with the stop block 43, the impact force enables the screen 22 to vibrate, the centrifugal effect is improved, the stop block 43 moves downward along the sliding groove of the stop rod 42 after being impacted by the knocking block 41, the knocking block 41 smoothly passes through the stop block 43, and after the knocking block 41 is separated from the stop block 43, the stop block 43 returns to the original position under the elastic force of the spring II to prepare for the next impact.
The box body 1 is provided with a plugging module 5 at the position of the discharge port, a disc-shaped groove is horizontally arranged in the box body 1 at the position of the discharge port, the diameter of the disc-shaped groove is larger than that of the discharge port, and the center of the disc-shaped groove deviates from the discharge port; the plugging module 5 comprises a plugging plate 51, a rotating shaft and a plugging motor 52, wherein the plugging plate 51 is positioned in a disc-shaped groove at the bottom of the box body 1, a through hole is eccentrically arranged on the plugging plate 51, and the plugging plate 51 rotates through the rotating shaft and the plugging motor 52, so that the through hole on the plugging plate 51 rotates to a position coaxial with the discharge hole, and the collection of bacteria after separation is realized. When bacteria falling to the bottom of the box body 1 after purification need to be collected, the plugging motor 52 drives the rotating shaft to rotate so as to enable the plugging plate 51 to move, the plugging plate 51 moves so as to enable the through hole in the plugging plate 51 to rotate to a position coaxial with the discharge port, and the bacteria liquid smoothly flows out through the discharge port.
The specific working process is as follows:
During operation, the transmission motor 33 drives the bevel gear 31 to coaxially rotate, the bevel gear 31 rotates to enable the centrifugal cylinders 21 meshed with the bevel gear 31 to synchronously rotate, the rotating speed of each centrifugal cylinder 21 is increased step by step from bottom to top due to the different sizes of the bevel gear 31, the centrifugal cylinders 21 rotate to drive the screen 22 to rotate, the rotating speed of the uppermost centrifugal cylinder 21 is the fastest, the effect of first-stage centrifugation can be improved, the apertures of the screen 22 are gradually reduced from top to bottom, bacterial liquid is purified through three-stage centrifugation, and the purified bacteria fall to the bottom of the box body 1; when the centrifugal cylinder 21 rotates, the part of the bacterial liquid passing through the screen 22 is thrown onto the inner wall of the centrifugal cylinder 21, the bacterial liquid slides down along the inner wall of the centrifugal cylinder 21, and the bacterial liquid enters the screen 22 below along the guide plate 23 due to the existence of the guide plate 23, so that the centrifugal effect is improved; because the rotating speed difference exists between the downward adjacent centrifugal cylinders 21, the rotating speed of the centrifugal cylinder 21 above is higher than that of the centrifugal cylinder 21 below, the knocking block 41 collides when moving to contact with the stop block 43, the impact force enables the screen 22 to vibrate, the centrifugal effect is improved, the stop block 43 moves downward along the sliding groove of the stop rod 42 after being impacted by the knocking block 41, the knocking block 41 smoothly passes through the stop block 43, and after the knocking block 41 is separated from the stop block 43, the stop block 43 returns to the original position under the elastic force of the spring II to prepare for the next impact.
When bacteria falling to the bottom of the box body 1 after purification need to be collected, the plugging motor 52 drives the rotating shaft to rotate so as to enable the plugging plate 51 to move, the plugging plate 51 moves so as to enable the through hole in the plugging plate 51 to rotate to a position coaxial with the discharge port, and the bacteria liquid smoothly flows out through the discharge port.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.