CN108421645A - A kind of medicine bacterium multi-stage separation screening system - Google Patents

Abstract

The invention belongs to microbial testing technology fields, specifically a kind of medicine bacterium multi-stage separation screening system, including babinet, centrifugation module, transmission module and vibration module, babinet is the rectangular parallelepiped structure of build-in cavities, babinet is installed on ground by stabilizer blade, top of the box offers feed inlet, and the bottom of box is provided with discharge port；Centrifugation module is located in the cavity of babinet, and centrifugation module realizes the separation screening of bacterium for being centrifuged step by step to bacterium solution；Transmission module is located at the side of centrifugation module, and transmission module rotates for realizing the differential of centrifugation module；Vibration module is set in centrifugation module, and vibration module is used to avoid the blocking of centrifugation module.The present invention can carry out multistage differential centrifugation to bacterium liquid and realize screening, and centrifugal effect is good, work efficiency is high.

Description

A multi-stage separation and screening system for medical bacteria

technical field

The invention belongs to the technical field of microbiology inspection, and specifically relates to a multistage separation and screening system for medical bacteria.

Background technique

Bacteria isolation refers to the process of isolating specific bacteria from a mixture of bacteria and cells, or a mixture of various bacteria. It is of great significance in clinical medicine, food industry, water quality testing, bioengineering and pharmaceutical industries. The traditional bacterial isolation is carried out in agar medium, the culture conditions are harsh, time-consuming, and the isolation and culture process is easily contaminated by miscellaneous bacteria, so the bacteria isolation and culture based on this method often cannot obtain ideal results. However, the existing microbial separators can only perform one-stage separation, and the separation is not complete, which cannot meet the requirements of microbial separation, experiment requirements, and scientific research.

There is also a technical solution for bacterial separation in the prior art. For example, a Chinese patent with application number 201620075181 discloses a two-stage separation and screening device for medical microorganisms, which includes a frame, universal wheels, a microbial cylinder, Filter cylinder, secondary centrifugal cylinder, primary centrifugal cylinder, mounting plate, rotating shaft, driven pulley, driving pulley, belt, motor, gasket Ⅰ, gasket Ⅱ, centrifugal cylinder cover, inner pad, top cover, handle And the outlet, the microbial cylinder is connected to the top of the rack. The driving pulley is installed on the output shaft of the motor. The driven pulley is fixedly connected to the lower end of the rotating shaft, and the belt is sleeved on the driven pulley and the driving pulley. The upper end of the rotating shaft passes through the microbial cylinder body and the filter cylinder body, and its top end is fixedly connected with the mounting plate. The secondary centrifugal cylinder and the primary centrifugal cylinder are fixedly connected to the mounting plate by screws, the centrifuge cover is clamped on the top of the secondary centrifugal cylinder and the primary centrifugal cylinder, the inner pad is arranged between the microbial cylinder and the top cover, and the filter The cylinder body is set on the rotating shaft, the sealing gasket I is arranged between the microbial cylinder body and the rotating shaft, and the sealing gasket II is arranged between the filter cylinder body and the rotating shaft. Although this technical solution can realize the separation of bacteria through two-stage sieving, the rotational speed of this technical solution is the same when performing two-stage centrifugation. In order to achieve the desired effect, it needs to consume more energy. Utilize, work efficiency is low, makes the use of this invention be limited.

In view of this, the multi-stage separation and screening system for medical bacteria according to the present invention performs centrifugal screening on bacteria by setting multi-stage centrifugation with different rotating speeds, which has good centrifugation effect and high work efficiency.

Contents of the invention

In order to make up for the deficiencies of the prior art, the present invention proposes a multi-stage separation and screening system for medical bacteria. The present invention is mainly used to realize high-efficiency separation and screening of bacteria through multi-stage centrifugation. Through the mutual cooperation of the centrifugal module and the transmission module, the present invention can carry out multi-stage differential speed centrifugation on the bacterial liquid to realize screening, and the centrifugal effect is good.

The technical solution adopted by the present invention to solve the technical problem is: a multi-stage separation and screening system for medical bacteria according to the present invention, including a box body, a centrifugal module, a transmission module and a vibration module, and the box body is a built-in cavity. Cuboid structure, the box is installed on the ground through the feet, the top of the box is provided with a feed port, and the bottom of the box is provided with a discharge port; the centrifugal module is located in the cavity of the box, and the centrifugal module is used to process the bacterial liquid one by one. stage centrifugal to realize the separation and screening of bacteria; the transmission module is located on one side of the centrifugal module, and the transmission module is used to realize the differential rotation of the centrifugal module; the vibration module is arranged on the centrifugal module, and the vibration module is used to avoid the vibration of the centrifugal module. clogged.

The centrifugal module includes a centrifugal cylinder, a screen and a spring. The top, lower surface and bottom of the box are provided with a circular chute. The centrifugal cylinder is a circular truncated structure, and the inside of the circular pedestal is coaxially provided with a circular truncated through hole. The upper bottom surface of the round platform is smaller than the lower bottom surface, the number of centrifugal cylinders is at least three, and the centrifugal cylinders are placed vertically between them. The cylinders are connected by means of annular protrusions installed in the circular grooves of the lower centrifugal cylinders. Relative rotation can occur between the centrifugal cylinders. The upper end of the uppermost centrifugal cylinder is located in the circular chute of the box, and the bottom The lower end of the centrifugal cylinder is located in the circular chute at the bottom of the box, and the outer surface of the centrifugal cylinder is provided with a toothed structure; the number of the screen is the same as the number of the centrifugal cylinder, and the screen is horizontally installed inside the centrifugal cylinder through a spring , the pore size of the upper sieve is larger than the pore size of the lower sieve. When in use, the rotation of the centrifugal cylinder drives the screen to rotate, and the top centrifugal cylinder rotates at the fastest speed, which can improve the effect of the first stage of centrifugation. The aperture of the screen gradually decreases from top to bottom, and the bacterial liquid is purified by three-stage centrifugation The final bacteria fall to the bottom of the box.

The inner wall of the centrifugal cylinder is also provided with a guide plate, which is obliquely installed on the inner wall of the centrifugal cylinder, and the guide plate is used for the bacteria liquid adhered to the inner wall of the centrifugal cylinder to fall back into the screen below during centrifugation. When the centrifuge cylinder rotates, the bacterial liquid that passes through the screen is thrown onto the inner wall of the centrifugal cylinder, and the bacterial liquid slides down along the inner wall of the centrifugal cylinder. The existence of the guide plate makes the bacterial liquid enter the lower screen along the guide plate , to improve the centrifugal effect.

The transmission module includes bevel gears, transmission shafts, transmission motors and brackets. The number of bevel gears is the same as the number of centrifugal cylinders. The bevel gears are coaxially installed on the vertical transmission shaft. The upper bevel gear is larger than the lower bevel gear, and the bevel gear It meshes with the tooth structure on the outer surface of the centrifugal cylinder; the transmission motor is installed on the feet of the box body through a bracket, and the output shaft of the transmission motor is connected with the transmission shaft. When in use, the transmission motor drives the bevel gear to rotate coaxially, and the rotation of the bevel gear makes the centrifugal cylinders meshed with it rotate synchronously. Due to the different sizes of the bevel gears, the rotational speed of each centrifugal cylinder increases step by step from bottom to top.

The vibration module includes a knocking block, a blocking rod, a stopper and a spring two, the knocking block is installed on the bottom of the screen; the blocking rod is installed on the screen below, and the blocking rod is located above the screen below to block The upper surface of the bar is vertically provided with a slide groove, and a spring two is installed in the slide groove; the stopper is located in the slide groove of the blocking rod, and the spring two is connected with the stopper, and the height of the stopper is higher than the height of the knocking block, and the stopper is The contact surface between the block and the knocking block is an inclined surface, and the contact position between the knocking block and the stopper is arc-shaped. Due to the speed difference between the downward adjacent centrifugal cylinders, the rotational speed of the upper centrifugal cylinder is higher than the rotational speed of the lower centrifugal cylinder, and the knocking block moves to contact with the stopper and collides, and the impact force causes the screen to vibrate, improving the centrifugal force. After being hit by the knocking block, the stopper moves down along the sliding groove of the blocking rod, so that the knocking block passes through the stopper smoothly. After the knocking block is out of contact with the stopper, the stopper is under the elastic force of the second spring Return to the original position and prepare for the next impact.

The box body is provided with a plugging module at the position of the discharge port, and the box body is horizontally built with a disc-shaped groove at the position of the discharge port. The diameter of the disc-shaped groove is greater than the diameter of the discharge port, and the center of the disc groove Deviated from the discharge port; the blocking module includes a blocking plate, a rotating shaft and a blocking motor, the blocking plate is located in a disc-shaped groove at the bottom of the box, and a through hole is eccentrically arranged on the blocking plate to block the The plate is rotated by the rotating shaft and the blocking motor, so that the through hole on the blocking plate is rotated to the coaxial position with the discharge port, so as to realize the collection of bacteria after separation. When it is necessary to collect the bacteria that have fallen to the bottom of the box after purification, the plugging motor drives the rotating shaft to rotate to move the plugging plate, and the movement of the plugging plate makes the through hole on the plugging plate rotate to a position coaxial with the discharge port. The bacterial liquid flows out smoothly through the discharge port.

The beneficial effects of the present invention are:

1. A multi-stage separation and screening system for medical bacteria according to the present invention, the centrifugal module, the transmission module and the vibration module cooperate with each other, and the differential speed operation of the centrifugal cylinder is realized by using bevel gears, thereby realizing the multi-stage differential speed Centrifugation improves the effect of bacteria separation and energy utilization; at the same time, the vibration module uses the differential rotation of the centrifugal cylinder to regularly knock on the screen to avoid blockage of the screen.

2. A multi-stage separation and screening system for medical bacteria according to the present invention, the plugging module at the bottom of the box is provided with an eccentric through hole on the plugging plate, and the plugging plate rotates to realize the closure of the box body discharge port. Closed and opened, strong reliability, good sealing, avoiding bacterial contamination.

3. A kind of medical bacterium multistage separation and screening system of the present invention, described sieve is also provided with vibrating module, by respectively setting percussion block and stopper between the sieve adjacent up and down, utilizes between the sieve The differential movement between them realizes the regular knocking of the upper screen to the lower screen, which makes the screen vibrate, prevents the screen from being blocked, and improves the centrifugal effect.

Description of drawings

The present invention will be further described below in conjunction with accompanying drawing.

Fig. 1 is the front view of the present invention;

Fig. 2 is A-A sectional view among Fig. 1 of the present invention;

Fig. 3 is the B-B sectional view among Fig. 1 of the present invention;

In the figure: box 1, centrifugal module 2, transmission module 3, cleaning module 4, foot 11, centrifugal cylinder 21, screen 22, guide plate 23, bevel gear 31, transmission shaft 32, transmission motor 33, vibration module 4, Knocking block 41 , blocking rod 42 , block 43 , blocking module 5 , blocking plate 51 , and blocking motor 52 .

Detailed ways

In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

As shown in Figures 1 to 3, a multi-stage separation and screening system for medical bacteria according to the present invention includes a box body 1, a centrifugal module 2, a transmission module 3 and a vibration module 4, and the box body 1 is a built-in cavity The cuboid structure, the box body 1 is installed on the ground through the feet 11, the top of the box body 1 is provided with a feed port, and the bottom of the box body 1 is provided with a discharge port; the centrifugal module 2 is located in the cavity of the box body 1, and the centrifugal module 2 is used to centrifuge the bacterial liquid step by step to realize the separation and screening of bacteria; the transmission module 3 is located on one side of the centrifugal module 2, and the transmission module 3 is used to realize the differential rotation of the centrifugal module 2; the vibration module 4 is set On the centrifugal module 2 , the vibration module 4 is used to avoid clogging of the centrifugal module 2 .

The centrifugal module 2 includes a centrifugal cylinder 21, a screen 22 and a spring. The lower surface and the bottom of the top of the box 1 are provided with a circular chute. The centrifugal cylinder 21 is a circular platform structure, and the circular platform is coaxially provided with Round table-shaped through hole, the upper bottom surface of the round table is smaller than the lower bottom surface, the number of centrifugal cylinders 21 is at least three, vertically placed between the centrifugal cylinders 21, the lower bottom surface of the centrifugal cylinders 21 is provided with ring-shaped protrusions, and the upper bottom surface of the centrifugal cylinders 21 is correspondingly provided with Circular groove, the upper centrifugal cylinder 21 is installed in the circular groove of the lower centrifugal cylinder 21 through a circular protrusion to realize connection, relative rotation can occur between the centrifugal cylinders 21, the upper end of the uppermost centrifugal cylinder 21 Located in the annular chute of the box body 1, the lower end of the centrifugal cylinder 21 at the bottom is located in the annular chute at the bottom of the box body 1, and the outer surface of the centrifugal cylinder 21 is provided with a toothed structure; the screen 22 The number is the same as that of the centrifugal cylinder 21, and the screen cloth 22 is horizontally installed inside the centrifugal cylinder 21 by a spring, and the aperture of the upper screen cloth 22 is greater than the aperture of the lower screen cloth 22. When in use, the centrifugal cylinder 21 rotates to drive the screen 22 to rotate, and the top centrifugal cylinder 21 rotates at the fastest speed, which can improve the effect of the first stage of centrifugation. The aperture of the screen 22 gradually decreases from top to bottom, and the bacterial liquid passes through the third stage After centrifugal purification, the purified bacteria fall to the bottom of the casing 1 .

The inner wall of the centrifugal cylinder 21 is also provided with a guide plate 23, the guide plate 23 is installed obliquely on the inner wall of the centrifugal cylinder 21, and the guide plate 23 is used for the bacterium liquid adhering to the inner wall of the centrifugal cylinder 21 to fall back to the screen below when centrifuging Within 22. When the centrifuge cylinder 21 rotates, the bacterial liquid that passes through the screen 22 is thrown onto the inner wall of the centrifugal cylinder 21, and the bacterial liquid slides down along the inner wall of the centrifugal cylinder 21. The existence of the guide plate 23 makes the bacterial liquid follow the guide plate 23 Enter in the screen cloth 22 below, improve centrifugal effect.

The transmission module 3 includes a bevel gear 31, a transmission shaft 32, a transmission motor 33 and a support. The number of the bevel gear 31 is the same as that of the centrifugal cylinder 21. The bevel gear 31 is coaxially installed on the vertical transmission shaft 32. The bevel gear above 31 is larger than the bevel gear 31 below, and the bevel gear 31 meshes with the tooth structure on the outer surface of the centrifugal cylinder 21; the transmission motor 33 is installed on the leg 11 of the box body 1 through a bracket, and the output shaft of the transmission motor 33 and the transmission shaft 32 connected. When in use, the transmission motor 33 drives the bevel gear 31 to rotate coaxially, and the rotation of the bevel gear 31 makes the centrifuge cylinder 21 meshed with it rotate synchronously. Because the bevel gear 31 is different in size, the speed of each centrifuge cylinder 21 increases step by step from bottom to top. .

The vibration module 4 includes a knocking block 41, a blocking rod 42, a block 43 and a spring two, the knocking block 41 is installed on the bottom of the screen 22; the blocking rod 42 is installed on the screen 22 below, Stop bar 42 is positioned at the top of bottom screen cloth 22. Stop bar 42 upper surface is vertically provided with sliding groove, and spring two is installed in the sliding groove; Connected, the height of the block 43 is higher than that of the knocking block 41, the contact surface between the block 43 and the knocking block 41 is an inclined surface, and the contact position between the knocking block 41 and the block 43 is arc-shaped. Because there is a speed difference between the downwardly adjacent centrifugal cylinders 21, the rotational speed of the centrifugal cylinder 21 above is higher than that of the centrifugal cylinder 21 below, and the knocking block 41 moves to a collision with the stopper 43, and the impact force makes the screen mesh 22 generates vibrations to improve the centrifugal effect, and the stopper 43 moves downward along the sliding groove of the blocking rod 42 after being hit by the knocking block 41, so that the knocking block 41 passes through the stopper 43 smoothly, and the knocking block 41 and the stopper 43 After being out of contact, the stopper 43 returns to its original position under the elastic force of the second spring to prepare for the next impact.

The box body 1 is provided with a plugging module 5 at the position of the discharge port, and the box body 1 is horizontally built with a disc-shaped groove at the position of the discharge port, the diameter of the disc-shaped groove is greater than the diameter of the discharge port, and the disk The center of the groove deviates from the discharge port; the blocking module 5 includes a blocking plate 51, a rotating shaft and a blocking motor 52, and the blocking plate 51 is located in the disc-shaped groove at the bottom of the casing 1, and the blocking plate 51 The upper eccentric is provided with a through hole, and the blocking plate 51 is rotated by the rotating shaft and the blocking motor 52, so that the through hole on the blocking plate 51 is rotated to a position coaxial with the discharge port, so as to realize the collection of bacteria after separation. When it is necessary to collect and purify the bacteria that fall to the bottom of the box body 1, the plugging motor 52 drives the rotating shaft to rotate to make the plugging plate 51 move, and the plugging plate 51 moves so that the through hole on the plugging plate 51 rotates to the discharge port. In the coaxial position, the bacterial liquid flows out smoothly through the discharge port.

The specific workflow is as follows:

When working, the transmission motor 33 drives the bevel gear 31 to rotate coaxially, and the rotation of the bevel gear 31 makes the centrifuge cylinder 21 meshed with it rotate synchronously. Because the bevel gear 31 is different in size, the speed of each centrifuge cylinder 21 increases step by step from bottom to top , the rotation of the centrifuge cylinder 21 drives the rotation of the screen 22, and the rotation speed of the top centrifuge cylinder 21 is the fastest, which can improve the effect of the first-stage centrifugation. The aperture of the screen 22 gradually decreases from top to bottom, and the bacterial liquid is purified by three-stage centrifugation , the purified bacteria fall to the bottom of the box body 1; when the centrifuge cylinder 21 rotates, the bacterial liquid part passing through the screen 22 is thrown onto the inner wall of the centrifuge cylinder 21, and the bacterial liquid slides down along the inner wall of the centrifuge cylinder 21, guiding The presence of the plate 23 makes the bacterial liquid enter the screen 22 below along the guide plate 23, improving the centrifugation effect; due to the rotational speed difference between the downwardly adjacent centrifugal cylinders 21, the rotational speed of the upper centrifugal cylinder 21 is higher than that of the lower centrifugal cylinder 21 speed, the knocking block 41 moves to the contact with the block 43 and impacts, and the impact force causes the screen 22 to vibrate, improving the centrifugal effect, and the block 43 slides along the blocking rod 42 after being hit by the knocking block 41 Groove moves down, and knocking piece 41 is passed through stopper 43 smoothly, and after knocking piece 41 is out of contact with stopper 43, stopper 43 returns to original position under the elastic force of spring two, prepares for next time impact.

When it is necessary to collect and purify the bacteria that fall to the bottom of the box body 1, the plugging motor 52 drives the rotating shaft to rotate to make the plugging plate 51 move, and the plugging plate 51 moves so that the through hole on the plugging plate 51 rotates to the discharge port. In the coaxial position, the bacterial liquid flows out smoothly through the discharge port.

The basic principles, main features and advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Variations and improvements are possible, which fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.

Claims (6)

1. A multi-stage separation and screening system for medical bacteria, characterized in that: it comprises a casing (1), a centrifugal module (2), a transmission module (3) and a vibration module (4), and the casing (1) is a built-in The cuboid structure of the cavity, the box body (1) is installed on the ground through the feet (11), the top of the box body (1) is provided with a feed port, and the bottom of the box body (1) is provided with a discharge port; the centrifugal module (2 ) is located in the cavity of the box body (1), and the centrifugal module (2) is used to centrifuge the bacterial liquid step by step to realize the separation and screening of bacteria; the transmission module (3) is located on one side of the centrifugal module (2), The transmission module (3) is used to realize differential rotation of the centrifugal module (2); the vibration module (4) is arranged on the centrifugal module (2), and the vibration module (4) is used to avoid blockage of the centrifugal module (2). 2. A kind of medical bacteria multistage separation and screening system according to claim 1, characterized in that: the centrifugal module (2) comprises a centrifugal cylinder (21), a screen (22) and a spring one, and the casing (1) The top lower surface and the bottom are provided with a circular chute, the centrifugal cylinder (21) is a circular platform structure, the circular platform is coaxially provided with a circular platform-shaped through hole, the upper bottom surface of the circular platform is smaller than the lower surface, and the centrifugal cylinder (21) The number is at least three, the centrifugal cylinders (21) are vertically placed, the bottom surface of the centrifugal cylinders (21) is provided with an annular protrusion, the upper bottom surface of the centrifugal cylinders (21) is correspondingly provided with an annular groove, and the centrifugal cylinders (21) above are provided with annular grooves. The cylinder (21) is installed in the circular groove of the centrifugal cylinder (21) through the annular protrusion to realize the connection, and relative rotation can occur between the centrifugal cylinders (21), and the upper end of the centrifugal cylinder (21) at the top is located at In the circular chute of the casing (1), the lower end of the lowermost centrifugal cylinder (21) is located in the circular chute at the bottom of the casing (1), and the outer surface of the centrifugal cylinder (21) is provided with toothed Structure; the number of the screen (22) is the same as that of the centrifugal cylinder (21), the screen (22) is horizontally installed inside the centrifugal cylinder (21) by a spring, and the aperture of the upper screen (22) is larger than that of the lower screen ( 22) Aperture. 3. A multi-stage separation and screening system for medical bacteria according to claim 2, characterized in that: a guide plate (23) is also provided on the inner wall of the centrifugal cylinder (21), and the guide plate (23) is installed obliquely on the centrifuge On the inner wall of the cylinder (21), the guide plate (23) is used for centrifugation when the bacteria liquid adhering to the inner wall of the centrifugal cylinder (21) falls back in the screen cloth (22) below. 4. A kind of medical bacteria multi-stage separation and screening system according to claim 2, characterized in that: the transmission module (3) comprises a bevel gear (31), a transmission shaft (32), a transmission motor (33) and a support , the number of bevel gears (31) is the same as the number of centrifugal cylinders (21), the bevel gears (31) are coaxially installed on the vertical transmission shaft (32), and the bevel gears (31) above are larger than the bevel gears (31) below , the bevel gear (31) meshes with the toothed structure on the outer surface of the centrifugal cylinder (21); The shaft is connected with the transmission shaft (32). 5. a kind of medical bacteria multi-stage separation and screening system according to claim 2, is characterized in that: described vibration module (4) comprises knock block (41), blocking bar (42), stopper (43) and Spring two, the knocking block (41) is installed on the bottom of the screen (22); the blocking rod (42) is installed on the screen (22) below, and the blocking rod (42) is located at the bottom of the screen (22). ) is vertically provided with a sliding groove on the upper surface of the upper blocking rod (42), and spring two is installed in the sliding groove; ) is connected, the height of the block (43) is higher than the height of the knocking block (41), the contact surface of the block (43) and the knocking block (41) is an inclined surface, and the knocking block (41) and the block ( 43) The contact position is arc-shaped. 6. A kind of medical bacteria multi-stage separation and screening system according to claim 1, characterized in that: said casing (1) is provided with a plugging module (5) at the position of the outlet, and the casing (1) A disc-shaped groove is built-in horizontally at the position of the discharge port, the diameter of the disc-shaped groove is greater than the diameter of the discharge port, and the center of the disc groove deviates from the discharge port; the plugging module (5) includes a plugging plate ( 51), the rotating shaft and the plugging motor (52), the plugging plate (51) is located in the disc-shaped groove at the bottom of the box body (1), and a through hole is eccentrically arranged on the plugging plate (51) to block the The plate (51) is rotated by the rotating shaft and the plugging motor (52), so that the through hole on the plugging plate (51) is rotated to a position coaxial with the discharge port, so as to realize the collection of bacteria after separation.