CN108403756A - A kind of pet anti parasitic compound preparation - Google Patents
A kind of pet anti parasitic compound preparation Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A61P33/10—Anthelmintics
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- A61P33/10—Anthelmintics
- A61P33/12—Schistosomicides
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A61K2236/30—Extraction of the material
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The invention discloses a kind of pet anti parasitic compound preparations, belong to veterinary drug preparation preparing technical field.The present invention is using ganoderma lipsiense as raw material, the extract with β carbonic anhydrase inhibitory activity is made, the enzymatic activity can effectively be inhibited, there is extensive killing effect to pet endoparasite, and on pet without influence, coordinate zinc fingers possessed by the heat shock protein in Escherichia coli DnaJ protein liquids, it can be with β carbonic anhydrase competitive bindings Zn2+Achieve the effect that Reverse transcriptase, improve the killing effect to parasite, a variety of alkaloids such as matrine, oxymatrine, the Sophoridine contained in cooperation shrubby sophora extract can be by the nervous system of destruction polypide, cause polypide Antifeedant Effects, play expelling parasite insecticidal effect, reach notable expelling parasite insecticidal effect, and is not likely to produce drug resistance.The present invention solve it is single for the current antiparasitic formulations product structure of animal in the market, to generate drug resistance, and to the poor problem of the control effect of parasite.
Description
Technical field
The invention belongs to veterinary drug preparation preparing technical field, more particularly to a kind of pet anti parasitic compound preparation.
Background technology
With the development of the social economy, people’s lives level is higher and higher, pet industry has also obtained unprecedented development, with
It pet and people’s lives is more and more closer, the prevention of pet parasitic disease is also increasingly valued by people.For a long time with
Come, anti parasitic class chemicals are almost relied on to the control of animal parasitosis.These drugs shortly after use,
Arthropod is just almost bar none to which create different degrees of drug resistance, and that there is also toxicity is big, long-term for chemicals
The disadvantages such as residual in livestock products and environment.Animal parasitosis is the main original for causing domestic and international animal husbandry huge economic losses
One of because.Currently, domestic ectoparasite disease mainly or relies on medical treatment, wherein chemicals are still leading at present anti-
Control means.In recent years, due to a variety of different type parasite mixing senses such as nematode, tapeworm, fluke and various vermins
Dye increases, and causes the therapeutic effect of folk prescription anti-parasite medicine undesirable.Ivermectin is semi-synthetic macrolides wide spectrum
Antiparasitic agent kills effect to internal epizoa especially nematode and vermin with good, but to tapeworm, suction
Worm and protozoan are invalid；Praziquantel is pyrazinoisoquinoline compound, which has special efficacy to snail fever, while also to tapeworm
Adult, larva it is effective, but praziquantel is invalid to nematode and vermin.Therefore, as can working out rational preparation work
Anti-parasitic compound preparation is made in drug by skill, and the pest-resistant spectrum of the two will be made to form perfect complementation, formed to nematode, tapeworm, suction
The effective anti-parasitic compound preparation of a variety of different type parasitic infections such as worm and various vermins.However,
China's veterinary drug clinic does not have ripe anti-parasitic compound preparation also, though developed countries have many compound anti-parasitic systems
Agent, such as ivermectin+Pyrantel compound preparations of Merial companies, praziquantel+Pyrantel+Fei Bantuo compounds of Bayer companies
Preparation, Mile companies praziquantel+Pyrantel compound preparation etc., but do not have ideal anti-parasitic compound preparation yet so far
Listing.
Invention content
The technical problems to be solved by the invention：For the current antiparasitic formulations product structure list of animal in the market
One, to generate drug resistance, and to the poor problem of the control effect of parasite, provide a kind of pet anti parasitic compound preparation.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of pet anti parasitic compound preparation includes following component according to the mass fraction：6 ~ 15 parts of essence, 4 ~ 10 portions of sucrose, 5 ~
10 parts of auxiliary agents, 20 ~ 40 parts of water, which is characterized in that further include 20 ~ 30 parts of shrubby sophora extracts, 30 ~ 50 parts of compound enzyme inhibitors；
The preparation method of the shrubby sophora extract：It takes kuh-seng to dry, pulverize sieving, takes sieving particle in mass ratio 1：4 ~ 6 are added
Methanol impregnates 2 ~ 3h, and in 50 ~ 60 DEG C of ultrasonic wave extractions, filtering takes filtrate, rotary evaporation, be concentrated under reduced pressure into original volume 20 ~
30%, concentrate is obtained, concentrate in mass ratio 1 is taken：3 ~ 4 are added acetic acid solution mixing, stir, and filtering takes filtrate, is concentrated under reduced pressure,
Medicinal extract is obtained, it is dry, obtain shrubby sophora extract；
The preparation method of the compound enzyme inhibitor, includes the following steps：
（1）Ganoderma lipsiense cleaning, drying are taken, are pulverized and sieved, sieving particle in mass ratio 1 is taken：2 ~ 3 are added water, obtain ganoderma lipsiense
0.3% cellulase of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5%, at 28 ~ 35 DEG C, enzyme is added in liquid
2 ~ 3h is solved, enzymolysis liquid is obtained；
（2）In 28 ~ 35 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, shaking table shaken cultivation 4 ~ 6 days obtains just
Culture solution takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 5 ~ 7 days, obtain zymotic fluid, zymotic fluid is taken to centrifuge,
It takes supernatant to be evaporated under reduced pressure to the 25 ~ 40% of original volume, obtains fermented concentrate；
（3）In mass ratio 1：5 ~ 8 take Escherichia coli DnaJ protein liquids, acetic acid solution mixing, stand, diluted protein solution are obtained, by matter
Measure ratio 2：1 takes diluted protein solution to be uniformly mixed with fermented concentrate to get compound enzyme inhibitor.
The auxiliary agent：In mass ratio 4：1 ~ 2, extracting corn starch, peanut oil mixing are to get auxiliary agent.
The step（2）In seed culture medium：According to the mass fraction, take 20 ~ 30 parts of wheat bran, 6 ~ 15 parts of glucose, 8 ~
16 parts of yeast extracts, 1000 parts of water, the 15 ~ 20min that sterilizes in 121 DEG C is to get fermentation medium.
The step（2）In fermentation medium：According to the mass fraction, 20 ~ 30 portions of potatos, 6 ~ 15 parts of fructose, 3 ~ 8 are taken
Part NaNO3, 4 ~ 8 parts（NH4)2SO4, 0.4 ~ 0.9 part of vitamin B1, 1000 parts of water mixing, sterilize in 121 DEG C 15 ~ 20min to get
Fermentation medium.
Compared with other methods, advantageous effects are the present invention：
The present invention is using ganoderma lipsiense as raw material, and through operations such as crushing, enzymolysis, fermented and cultureds, being made, there is β-carbonic anhydrase to inhibit
Active extract can effectively inhibit the enzymatic activity, and β-carbonic anhydrase is a kind of metalloenzyme, to pathogenic bacteria and parasite
Class metabolism is essential, is catalyzed CO2Aquation, participate in many physiology and pathologic process, including breathing, pH and CO2Stable state,
But without this enzyme in vertebrate and chordate animal body, therefore there can be extensive killing effect to pet endoparasite, and
On pet without influence, coordinate zinc fingers possessed by the heat shock protein in Escherichia coli DnaJ protein liquids, it can be with β-carbonic acid
Acid anhydride enzyme competitive binding Zn2+Achieve the effect that Reverse transcriptase, improve the killing effect to parasite, coordinates and contain in shrubby sophora extract
A variety of alkaloids such as some matrines, oxymatrine, Sophoridine can cause polypide food refusal by the nervous system of destruction polypide
Effect, plays expelling parasite insecticidal effect, reaches notable expelling parasite insecticidal effect, and be not likely to produce drug resistance.
Specific implementation mode
Auxiliary agent：In mass ratio 4：1 ~ 2, extracting corn starch, peanut oil mixing are to get auxiliary agent.
Seed culture medium：According to the mass fraction, 20 ~ 30 parts of wheat bran, 6 ~ 15 parts of glucose, 8 ~ 16 parts of yeast extracts, 1000 are taken
Part water, the 15 ~ 20min that sterilize in 121 DEG C are to get fermentation medium.
Fermentation medium：According to the mass fraction, 20 ~ 30 portions of potatos, 6 ~ 15 parts of fructose, 3 ~ 8 parts of NaNO are taken3, 4 ~ 8 parts
（NH4)2SO4, 0.4 ~ 0.9 part of vitamin B1, 1000 parts of water mixing, the 15 ~ 20min that sterilize in 121 DEG C are to get fermentation medium.
The preparation method of shrubby sophora extract：Sophora flavescens cleaning is taken, in 60 ~ 75 DEG C of oven dryings, 80 mesh sieve is crushed, takes sieving
Particle in mass ratio 1：4 ~ 6, which are added methanol, impregnates 2 ~ 3h, 40 ~ 60min of ultrasonic wave extraction, mistake under 50 ~ 60 DEG C, 300W power
Filter, takes filter residue, rotary evaporation to be concentrated under reduced pressure into the 20 ~ 30% of original volume, obtain concentrate, takes concentrate in mass ratio 1：3 ~ 4 add
Enter the acetic acid solution that mass fraction is 10% to mix, stir, filtering takes filtrate, is concentrated under reduced pressure, obtains medicinal extract, is done at 60 ~ 75 DEG C
It is dry to water content 5% hereinafter, shrubby sophora extract.
The preparation method of compound enzyme inhibitor, includes the following steps：
（1）It takes ganoderma lipsiense to clean, dry, pulverize 60 mesh sieve in 60 ~ 75 DEG C of drying boxes, and took sieving particle in mass ratio 1：2~
3 are added water, obtain ganoderma lipsiense liquid, and 0.3% cellulose of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5% is added
Enzyme digests 2 ~ 3h, obtains enzymolysis liquid at 28 ~ 35 DEG C；
（2）In 28 ~ 35 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, is vibrated with 160 ~ 230r/min shaking tables
Culture 4 ~ 6 days obtains just culture solution, takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 5 ~ 7 days, must ferment
Liquid is taken zymotic fluid to be centrifuged 10 ~ 15 minutes with 4000 ~ 5000r/min, takes supernatant to be evaporated under reduced pressure to the 25 ~ 40% of original volume, obtain
Fermented concentrate；
（3）In mass ratio 1：5 ~ 8 take Escherichia coli DnaJ protein liquids, the acetic acid solution that mass fraction is 8% mixes, 15 ~ 20 DEG C of perseverances
Temperature stands 30 ~ 50min, obtains diluted protein solution, in mass ratio 2：1 take diluted protein solution to be uniformly mixed with fermented concentrate to get
Compound enzyme inhibitor.
A kind of preparation method of pet anti parasitic compound preparation, includes the following steps：
（1）According to the mass fraction, take 30 ~ 50 parts of compound enzyme inhibitors, 20 ~ 30 parts of shrubby sophora extracts, 6 ~ 15 parts of essence, 4 ~ 10 parts
Sucrose, 5 ~ 10 parts of auxiliary agents, 20 ~ 40 parts of water；
（2）In 40 ~ 65 DEG C of water-bath, shrubby sophora extract, essence, auxiliary agent, water are taken, is mixed in container, is stirred with 250 ~ 300r/min
30 ~ 50min is cooled to 20 ~ 25 DEG C, and complex enzyme inhibitor mixed is added, and ultrasonic wave disperses 10 ~ 15min to get the anti-parasitism of pet
Worm compound preparation.
Auxiliary agent：In mass ratio 4：1, extracting corn starch, peanut oil mixing are to get auxiliary agent.
Seed culture medium：According to the mass fraction, 20 parts of wheat bran, 6 parts of glucose, 8 parts of yeast extracts, 1000 parts of water are taken, in 121
DEG C sterilizing 15min to get fermentation medium.
Fermentation medium：According to the mass fraction, 20 portions of potatos, 6 parts of fructose, 3 parts of NaNO are taken3, 4 parts（NH4)2SO4、0.4
Part vitamin B1, 1000 parts of water mixing, the 15min that sterilize in 121 DEG C are to get fermentation medium.
The preparation method of shrubby sophora extract：Sophora flavescens cleaning is taken, in 60 DEG C of oven dryings, 80 mesh sieve is crushed, takes sieving
Grain in mass ratio 1：4, which are added methanol, impregnates 2h, the ultrasonic wave extraction 40min under 50 DEG C, 300W power, and filtering takes filter residue, rotates
Evaporation, is concentrated under reduced pressure into the 20% of original volume, obtains concentrate, take concentrate in mass ratio 1：3 are added the second that mass fraction is 10%
Acid solution mixing, is stirred, and filtering takes filtrate, is concentrated under reduced pressure, obtains medicinal extract, is dried at 60 DEG C to water content 5% hereinafter, obtaining bitter
Conopsea extraction.
The preparation method of compound enzyme inhibitor, includes the following steps：
（1）It takes ganoderma lipsiense to clean, dry, pulverize 60 mesh sieve in 60 DEG C of drying boxes, and took sieving particle in mass ratio 1：2 are added
Water obtains ganoderma lipsiense liquid, and 0.3% cellulase of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5% is added, in
At 28 DEG C, 2h is digested, enzymolysis liquid is obtained；
（2）In 28 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, with 160r/min shaking tables shaken cultivation 4
It, obtains just culture solution, takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 5 days, obtain zymotic fluid, take zymotic fluid
It is centrifuged 10 minutes with 4000r/min, takes supernatant to be evaporated under reduced pressure to the 25% of original volume, obtain fermented concentrate；
（3）In mass ratio 1：5 take Escherichia coli DnaJ protein liquids, the acetic acid solution that mass fraction is 8% mixes, and 15 DEG C of constant temperature are quiet
30min is set, diluted protein solution, in mass ratio 2 are obtained：1 takes diluted protein solution to be uniformly mixed with fermented concentrate presses down to get complex enzyme
Preparation.
A kind of preparation method of pet anti parasitic compound preparation, includes the following steps：
（1）According to the mass fraction, take 30 parts of compound enzyme inhibitors, 20 parts of shrubby sophora extracts, 6 parts of essence, 4 portions of sucrose, 5 parts help
Agent, 20 parts of water；
（2）In 40 DEG C of water-bath, shrubby sophora extract, essence, auxiliary agent, water are taken, is mixed in container, 30min, drop are stirred with 250r/min
Complex enzyme inhibitor mixed is added to 20 DEG C in temperature, and ultrasonic wave disperses 10min to get pet anti parasitic compound preparation.
Auxiliary agent：In mass ratio 4：1.5, extracting corn starch, peanut oil mixing are to get auxiliary agent.
Seed culture medium：According to the mass fraction, 25 parts of wheat bran, 10 parts of glucose, 12 parts of yeast extracts, 1000 parts of water are taken, in
121 DEG C of sterilizing 18min are to get fermentation medium.
Fermentation medium：According to the mass fraction, 25 portions of potatos, 10 parts of fructose, 5 parts of NaNO are taken3, 6 parts（NH4)2SO4、
0.6 part of vitamin B1, 1000 parts of water mixing, the 18min that sterilize in 121 DEG C are to get fermentation medium.
The preparation method of shrubby sophora extract：Sophora flavescens cleaning is taken, in 68 DEG C of oven dryings, 80 mesh sieve is crushed, takes sieving
Grain in mass ratio 1：5, which are added methanol, impregnates 2.5h, the ultrasonic wave extraction 50min under 55 DEG C, 300W power, and filtering takes filter residue, revolves
Turn evaporation, is concentrated under reduced pressure into the 25% of original volume, obtains concentrate, take concentrate in mass ratio 1：3.5 addition mass fractions are 10%
Acetic acid solution mixing, stir, filtering, take filtrate, be concentrated under reduced pressure, obtain medicinal extract, at 68 DEG C dry to water content 5% hereinafter,
Obtain shrubby sophora extract.
The preparation method of compound enzyme inhibitor, includes the following steps：
（1）It takes ganoderma lipsiense to clean, dry, pulverize 60 mesh sieve in 68 DEG C of drying boxes, and took sieving particle in mass ratio 1：2.5 plus
Enter water, obtain ganoderma lipsiense liquid, 0.3% cellulase of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5% is added,
At 32 DEG C, 2.5h is digested, enzymolysis liquid is obtained；
（2）In 32 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, with 200r/min shaking tables shaken cultivation 5
It, obtains just culture solution, takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 6 days, obtain zymotic fluid, take zymotic fluid
It is centrifuged 13 minutes with 4500r/min, takes supernatant to be evaporated under reduced pressure to the 32% of original volume, obtain fermented concentrate；
（3）In mass ratio 1：7 take Escherichia coli DnaJ protein liquids, the acetic acid solution that mass fraction is 8% mixes, and 18 DEG C of constant temperature are quiet
40min is set, diluted protein solution, in mass ratio 2 are obtained：1 takes diluted protein solution to be uniformly mixed with fermented concentrate presses down to get complex enzyme
Preparation.
A kind of preparation method of pet anti parasitic compound preparation, includes the following steps：
（1）According to the mass fraction, take 40 parts of compound enzyme inhibitors, 25 parts of shrubby sophora extracts, 10 parts of essence, 7 portions of sucrose, 8 parts help
Agent, 30 parts of water；
（2）In 55 DEG C of water-bath, shrubby sophora extract, essence, auxiliary agent, water are taken, is mixed in container, 40min, drop are stirred with 280r/min
Complex enzyme inhibitor mixed is added to 23 DEG C in temperature, and ultrasonic wave disperses 13min to get pet anti parasitic compound preparation.
Auxiliary agent：In mass ratio 4：2, extracting corn starch, peanut oil mixing are to get auxiliary agent.
Seed culture medium：According to the mass fraction, 30 parts of wheat bran, 15 parts of glucose, 16 parts of yeast extracts, 1000 parts of water are taken, in
121 DEG C of sterilizing 20min are to get fermentation medium.
Fermentation medium：According to the mass fraction, 30 portions of potatos, 15 parts of fructose, 8 parts of NaNO are taken3, 8 parts（NH4)2SO4、
0.9 part of vitamin B1, 1000 parts of water mixing, the 20min that sterilize in 121 DEG C are to get fermentation medium.
The preparation method of shrubby sophora extract：Sophora flavescens cleaning is taken, in 75 DEG C of oven dryings, 80 mesh sieve is crushed, takes sieving
Grain in mass ratio 1：6, which are added methanol, impregnates 3h, the ultrasonic wave extraction 60min under 60 DEG C, 300W power, and filtering takes filter residue, rotates
Evaporation, is concentrated under reduced pressure into the 30% of original volume, obtains concentrate, take concentrate in mass ratio 1：4 are added the second that mass fraction is 10%
Acid solution mixing, is stirred, and filtering takes filtrate, is concentrated under reduced pressure, obtains medicinal extract, is dried at 75 DEG C to water content 5% hereinafter, obtaining bitter
Conopsea extraction.
The preparation method of compound enzyme inhibitor, includes the following steps：
（1）It takes ganoderma lipsiense to clean, dry, pulverize 60 mesh sieve in 75 DEG C of drying boxes, and took sieving particle in mass ratio 1：3 are added
Water obtains ganoderma lipsiense liquid, and 0.3% cellulase of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5% is added, in
At 35 DEG C, 3h is digested, enzymolysis liquid is obtained；
（2）In 35 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, with 230r/min shaking tables shaken cultivation 6
It, obtains just culture solution, takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 7 days, obtain zymotic fluid, take zymotic fluid
It is centrifuged 15 minutes with 5000r/min, takes supernatant to be evaporated under reduced pressure to the 40% of original volume, obtain fermented concentrate；
（3）In mass ratio 1：8 take Escherichia coli DnaJ protein liquids, the acetic acid solution that mass fraction is 8% mixes, and 20 DEG C of constant temperature are quiet
50min is set, diluted protein solution, in mass ratio 2 are obtained：1 takes diluted protein solution to be uniformly mixed with fermented concentrate presses down to get complex enzyme
Preparation.
A kind of preparation method of pet anti parasitic compound preparation, includes the following steps：
（1）According to the mass fraction, take 50 parts of compound enzyme inhibitors, 30 parts of shrubby sophora extracts, 15 parts of essence, 10 portions of sucrose, 10 parts
Auxiliary agent, 40 parts of water；
（2）In 65 DEG C of water-bath, shrubby sophora extract, essence, auxiliary agent, water are taken, is mixed in container, 50min, drop are stirred with 300r/min
Complex enzyme inhibitor mixed is added to 25 DEG C in temperature, and ultrasonic wave disperses 15min to get pet anti parasitic compound preparation.
Comparative example 1：It is essentially identical with the preparation method of embodiment 3, it has only the difference is that lacking shrubby sophora extract.
Comparative example 2：It is essentially identical with the preparation method of embodiment 3, it has only the difference is that lacking compound enzyme inhibitor.
Comparative example 3：The anti parasitic compound preparation of Yibin City company production.
Anti parasitic compound preparation prepared by embodiment 1,2,3 and comparative example 1,2,3,4 is respectively to suffering from liver rot, mite
The pet dog of worm is tested.Its test result is recorded in table 1.
Table 1：
| Test event | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Kill shortest time/day of liver fluke | 17 | 15 | 13 | 30 | 28 | 25 |
| The shortest time of mite killing/day | 0.6 | 1 | 0.8 | 7 | 6 | 9 |
| Control effect/% | 97 | 95 | 98 | 72 | 69 | 80 |
In conclusion as shown in Table 1, anti parasitic compound preparation of the invention is to dynamic product structure comparatively perfect, and to parasite
Control effect it is preferable, have preferable development prospect.
Claims (4)
1. a kind of pet anti parasitic compound preparation includes following component according to the mass fraction：6 ~ 15 parts of essence, 4 ~ 10 parts of sucrose, 5
~ 10 parts of auxiliary agents, 20 ~ 40 parts of water, which is characterized in that further include 20 ~ 30 parts of shrubby sophora extracts, 30 ~ 50 parts of compound enzyme inhibitors；
The preparation method of the shrubby sophora extract：It takes kuh-seng to dry, pulverize sieving, takes sieving particle in mass ratio 1：4 ~ 6 are added
Methanol impregnates 2 ~ 3h, and in 50 ~ 60 DEG C of ultrasonic wave extractions, filtering takes filtrate, rotary evaporation, be concentrated under reduced pressure into original volume 20 ~
30%, concentrate is obtained, concentrate in mass ratio 1 is taken：3 ~ 4 are added acetic acid solution mixing, stir, and filtering takes filtrate, is concentrated under reduced pressure,
Medicinal extract is obtained, it is dry, obtain shrubby sophora extract；
The preparation method of the compound enzyme inhibitor, includes the following steps：
（1）Ganoderma lipsiense cleaning, drying are taken, are pulverized and sieved, sieving particle in mass ratio 1 is taken：2 ~ 3 are added water, obtain ganoderma lipsiense
0.3% cellulase of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5%, at 28 ~ 35 DEG C, enzyme is added in liquid
2 ~ 3h is solved, enzymolysis liquid is obtained；
（2）In 28 ~ 35 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, shaking table shaken cultivation 4 ~ 6 days obtains just
Culture solution takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 5 ~ 7 days, obtain zymotic fluid, zymotic fluid is taken to centrifuge,
It takes supernatant to be evaporated under reduced pressure to the 25 ~ 40% of original volume, obtains fermented concentrate；
（3）In mass ratio 1：5 ~ 8 take Escherichia coli DnaJ protein liquids, acetic acid solution mixing, stand, diluted protein solution are obtained, by matter
Measure ratio 2：1 takes diluted protein solution to be uniformly mixed with fermented concentrate to get compound enzyme inhibitor.
2. pet anti parasitic compound preparation according to claim 1, which is characterized in that the auxiliary agent：In mass ratio 4：1 ~ 2,
Extracting corn starch, peanut oil mixing are to get auxiliary agent.
3. pet anti parasitic compound preparation according to claim 1, which is characterized in that the step（2）In seed culture
Base：According to the mass fraction, 20 ~ 30 parts of wheat bran, 6 ~ 15 parts of glucose, 8 ~ 16 parts of yeast extracts, 1000 parts of water are taken, in 121 DEG C of sterilizings
15 ~ 20min is to get fermentation medium.
4. pet anti parasitic compound preparation according to claim 1, which is characterized in that the step（2）In fermented and cultured
Base：According to the mass fraction, 20 ~ 30 portions of potatos, 6 ~ 15 parts of fructose, 3 ~ 8 parts of NaNO are taken3, 4 ~ 8 parts（NH4)2SO4, 0.4 ~ 0.9 part
Vitamin B1, 1000 parts of water mixing, the 15 ~ 20min that sterilize in 121 DEG C are to get fermentation medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810568158.7A CN108403756A (en) | 2018-06-05 | 2018-06-05 | A kind of pet anti parasitic compound preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810568158.7A CN108403756A (en) | 2018-06-05 | 2018-06-05 | A kind of pet anti parasitic compound preparation |
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| CN108403756A true CN108403756A (en) | 2018-08-17 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101015598A (en) * | 2007-02-14 | 2007-08-15 | 王东辉 | Externally used pharmaceutical preparation for treating disease caused by acarids |
| CN101389764A (en) * | 2005-08-01 | 2009-03-18 | Abr有限公司 | Carbohydrate compositions from basidiomycete fungi as biocidal agents active against pathogens |
| CN102028644A (en) * | 2009-09-30 | 2011-04-27 | 湖北汇特生物医药技术有限公司 | Bathing article based on medicinal property of related extraction parts of mugwort |
| CN104069217A (en) * | 2014-07-18 | 2014-10-01 | 青岛科维源环保技术有限公司 | Mongolian medicine for pet dog anti-parasitism and preparation method of anaesthetic preparation |
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- 2018-06-05 CN CN201810568158.7A patent/CN108403756A/en not_active Withdrawn
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101389764A (en) * | 2005-08-01 | 2009-03-18 | Abr有限公司 | Carbohydrate compositions from basidiomycete fungi as biocidal agents active against pathogens |
| CN101015598A (en) * | 2007-02-14 | 2007-08-15 | 王东辉 | Externally used pharmaceutical preparation for treating disease caused by acarids |
| CN102028644A (en) * | 2009-09-30 | 2011-04-27 | 湖北汇特生物医药技术有限公司 | Bathing article based on medicinal property of related extraction parts of mugwort |
| CN104069217A (en) * | 2014-07-18 | 2014-10-01 | 青岛科维源环保技术有限公司 | Mongolian medicine for pet dog anti-parasitism and preparation method of anaesthetic preparation |
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| 苏利波，等。: ""苦参抗寄生虫的研究进展"", 《中国病原生物学杂志》 * |
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Application publication date: 20180817 |